JP2011001348A - Formulation for prevention or alleviation of alcohol drunkenness or hangover - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an agent for preventing or alleviating alcohol drunkenness or hangover which is highly safe, can be easily produced, and can be utilized for foods and drinks such as health foods or health-promoting foods (food with predetermined health use and food with nutrient function claims), drugs, or quasi-drugs and to provide a formulation containing the same.SOLUTION: The formulation for preventing or alleviating alcohol drunkenness or hangover includes as an active ingredient an extract obtained by extracting Glycyrrhiza with an organic solvent, an oil and fat, or a mixture thereof. A nonaqueous Glycyrrhiza solvent extract obtained by bringing an extract obtained by contact of Glycyrrhiza with a water-soluble organic solvent into further contact with an oil and fat is particularly desirable.

Description

  The present invention relates to a composition for preventing drunkenness or hangover, which can be used for foods and drinks such as health foods and health functional foods (food for specified health use, nutritional functional foods), pharmaceuticals or quasi drugs.

  Intoxication is caused by ethanol (ethyl alcohol) contained in alcohol, which is caused by the suppression of brain function, and drunkness caused by the action of acetaldehyde, an intermediate product of alcohol metabolism in the body. There are two types.

  Drunkness due to the action of ethanol is due to the occurrence of paralysis (suppression) of the brain depending on the amount of ethanol ingested by drinking. Since cerebral palsy begins with paralysis of high-level functions of the cerebrum, judgment, concentration, deterrence, etc. are first reduced. As a result, the low-level function of the brain (a function called so-called instinct) becomes a surface layer, resulting in a light excitement state, a so-called drunken state that makes you feel upset and feel better. Furthermore, if the cerebral palsy progresses, it may affect motor nerves and, in some cases, acute alcoholism. In addition, acetaldehyde generated when ethanol is decomposed in the body is a toxic substance as described later. When this accumulates in the blood, it can lead to conditions such as increased heart rate, vomiting, and flushing of the skin. This acetaldehyde-induced state is a symptom different from the former ethanol-induced drunkenness, and when it becomes excessive, it becomes a state called so-called sickness.

  In addition, acetaldehyde is a causative agent of hangover. When ethanol is ingested, it is decomposed into acetaldehyde by alcohol dehydrogenase in the body, and acetaldehyde is further decomposed into acetic acid by acetaldehyde dehydrogenase, and finally decomposed into water and carbon dioxide. And discharged. This acetaldehyde, which is an intermediate metabolite of ethanol, is extremely toxic, and the symptoms caused by its toxicity appear on the next day after drinking. In other words, the cause of hangover is not caused by ethanol itself, but by the residual of acetaldehyde, an intermediate metabolite in the body. Therefore, since the causative substance is different between ethanol-induced drunk and acetaldehyde-induced drunk, it is considered that substances and methods suitable for improving the symptoms are also different.

  Moderate drinking has the effect of increasing appetite and relieving stress, but excessive drinking not only shows symptoms of drunkness and hangover such as flushing of the face, headache, sleepiness and nausea, but also causes liver damage, pancreatitis, etc. Causes harmful effects. Several methods have been proposed for suppressing these symptoms. That is, a substance that lowers the alcohol (ethanol) concentration in blood (Patent Documents 1 to 3), a substance that reduces the toxicity of acetaldehyde (Patent Document 4), and the like are disclosed.

  Licorice is a plant belonging to the genus Glycyrrhiza, which is used for food and medicine (herbal medicine). The main varieties are Glycyrrhiza. Glabra, G. G. uralensis, G. Examples include inflators (G. inflata). All varieties contain glycyrrhizin (glycyrrhizic acid) which is a hydrophilic component.

  Among the extracts obtained from licorice, licorice non-aqueous solvent extract containing a large amount of licorice flavonoids and having a very small amount of glycyrrhizin has been found to be useful for the prevention and treatment of multiple risk factor syndrome. (Patent Document 5). Further, in recent research, it has been found that this licorice non-aqueous solvent extract has PPARγ ligand activity and that its active ingredient is a flavonoid (Patent Document 6). On the other hand, compounds contained in licorice non-aqueous solvent extracts, such as licorice hydrophobic flavonoids, are hardly soluble in water, and are easily solidified with organic solvent extracts, and the coloration progresses quickly. It is difficult to use because of the nature of significant changes. In order to solve this problem, it has been proposed to dissolve in medium-chain fatty acid triglycerides and stabilize the preparation (Patent Document 7), but the types and uses of extraction solvents for obtaining licorice non-aqueous solvent extracts There has been no detailed study of the relevance.

  In addition, functional health foods that are effective in preventing and preventing excessive intoxication, hangover, and hangover associated with alcohol drinking have been reported, comprising young powder near buckwheat and licorice or licorice extract as essential ingredients ( Patent Document 8). Furthermore, a health drink that effectively prevents and prevents drunkness, hangover, and hangover associated with drinking, which is composed of a glycoside of quercetin, a divalent metal ion and a licorice extract as essential components, has been reported ( Patent Document 9). In these two reports, it is described that glycyrrhizin in the licorice extract works as an active ingredient, and in Patent Document 9, the licorice extract alone promotes the metabolism of alcohol (ethanol). Is also not enough.

JP-A-5-170659 JP-A-6-256201 JP-A-11-228428 JP-A-6-40901 WO02 / 047699 WO03 / 037316 Japanese Patent No. 2794433 Japanese Patent No. 2996313 JP-A-6-14746

  An object of the present invention is to provide a drunk or hangover prevention / relief agent and a composition containing the same, which are highly safe and can be easily produced.

  Surprisingly, the present inventors have found that an extract obtained by contacting licorice with a non-aqueous solvent not only has an effect of preventing or reducing hangover, but also has an effect of preventing and reducing hangover. Thus, the present invention has been completed.

  That is, the present invention is a composition for preventing or reducing hangover or hangover containing a non-aqueous solvent extract of licorice as an active ingredient. In a more preferred embodiment, a non-aqueous solvent extract of licorice is obtained by contacting another extract of non-aqueous solvent with an extract obtained by contacting licorice with a first non-aqueous solvent. It is said composition that is.

  According to the present invention, it can be used for foods and drinks such as health foods and health function foods (special health foods, nutritive function foods), medicines or quasi drugs, for preventing or reducing hangovers or hangovers. A composition is provided.

  Hereinafter, embodiments of the present invention will be described in detail.

  The composition for preventing or reducing hangover or hangover of the present invention is a composition containing a non-aqueous solvent extract of licorice as an active ingredient.

  The non-aqueous solvent extract of licorice which is an active ingredient of the composition of the present invention can be obtained from licorice. The licorice material used as a raw material of the composition of the present invention is not limited as long as it is a plant belonging to the genus Glycyrrhiza, Glycyrrhiza. Glabra, G. et al. G. uralensis, G. Examples include inflators (G. inflata). Among them, G. has the widest distribution and rich food experience. Grubra licorice is preferred.

  The method for obtaining the non-aqueous solvent extract of licorice from licorice is not particularly limited. For example, licorice or a powder thereof can be obtained by extraction with a non-aqueous solvent. Alternatively, a hydrophilic component in licorice can be extracted and removed in advance using water or an aqueous alkaline solution, and then extracted by contacting the licorice residue or a dried licorice residue with a nonaqueous solvent. .

  The non-aqueous solvent used in the present invention is not particularly limited as long as it is a solvent other than water, and an organic solvent, an oil or fat, or a mixed solvent thereof can be preferably used. Although it does not restrict | limit especially as an organic solvent, The safe thing which can be used for manufacture, processing, etc. of a pharmaceutical, food, a food additive, etc. is preferable, for example, acetone, C1-C4 monohydric alcohol, glycerol, fatty acid ester, Examples include diethyl ether, cyclohexane, propylene glycol and the like, and at least two of these solvents may be used in combination. Among these, examples of a solvent that can be suitably used include fatty acid esters such as ethyl acetate, monohydric alcohols having 1 to 4 carbon atoms, acetone, hexane, heptane, diethyl ether, cyclohexane, propylene glycol, and glycerin. Among these, water-soluble organic solvents such as monohydric alcohols having 1 to 4 carbon atoms, acetone, propylene glycol, and glycerin are preferable, and monohydric alcohols having 1 to 4 carbon atoms and acetone are more preferable among the water-soluble organic solvents. In particular, ethanol is preferred. The fats and oils are not particularly limited, and safe ones that can be used for the production and processing of pharmaceuticals, foods, food additives, etc. can be used. As described later, polyhydric alcohol derivatives such as polyhydric alcohol fatty acid esters are used. What is contained is preferable, and as the polyhydric alcohol fatty acid ester, a glycerin fatty acid ester containing a medium-chain fatty acid triglyceride or a glycerin fatty acid ester containing a fatty acid partial glyceride is more preferable. From such a point of view, as the fats and oils, medium chain fatty acid triglycerides, diglycerides and those containing them are particularly preferable.

  Extraction of licorice with a non-aqueous solvent can be carried out according to a general method, and is not particularly limited. The extraction temperature is not particularly limited, and can be suitably carried out in the range from the solidification temperature to the boiling point of the system, generally from -20 to 100 ° C, usually from 1 to 80 ° C, preferably from 20 to 60 ° C. The extraction operation is performed, for example, for 0.1 hour or more, preferably 0. The extraction may be performed for 2 hours or more, more preferably 0.5 hours or more. Usually, the extraction time per time is preferably about 1 to 10 hours. The upper limit is not particularly limited and is about one day, but may be performed for a longer time. The extraction may be performed once or a plurality of times as necessary, and the solvents to be used may be appropriately combined. The pressure at the time of extraction is not particularly limited. Normal pressure to pressurized state (1 to several atmospheres) can be used, but reduced pressure can be used if desired. It can also be carried out under reflux and in a slightly pressurized state.

  In the present invention, the non-aqueous solvent extract of licorice may use the obtained extract as it is, but further use a crudely purified or purified product by a purification process such as column treatment, deodorization treatment, decolorization treatment, etc. May be. The non-aqueous solvent extract of licorice contains a large amount of licorice polyphenol, which is a hydrophobic component of licorice. Furthermore, many licorice polyphenols have an antioxidant action and are characterized by having a prenylflavonoid group in the structure of the compound. The content of the licorice polyphenol having a prenylflavonoid group contained in the licorice non-aqueous solvent extract of the present invention is usually about 0.01 to 100% by weight, and 0.1 to 99% by weight. The thing containing 1 to 80 weight% is more preferable, The thing containing 3 to 50 weight% is further more preferable.

  In the composition for preventing / reducing hangover or hangover according to the present invention, the licorice polyphenol includes grabrene, grabridine, grabrol, 3′-hydroxy-4′-O-methylgrabridine, 4′-O-methylglabridine, Hispa. It preferably contains at least one compound selected from the group consisting of glabrizine B. The compound is a licorice, preferably G. Grubra seed licorice can be obtained by extracting with an organic solvent such as ethanol, hexane, acetone, etc., and the extract may be used as it is, but it is further purified by column treatment, deodorization treatment, decolorization treatment, etc. Alternatively, a purified product may be used. Of course, the compound may be any of those derived from other natural sources such as plants, and those that are chemically synthesized or biosynthesized by cultured cells. Moreover, although the refined | purified thing can be used for this compound, as long as it does not contain an unsuitable impurity as food / beverage products, a pharmaceutical, a quasi-drug, etc., it can also use the roughly refined | purified thing.

  Among these compounds, at least one selected from the group consisting of grabridine and comprising grabrene, grabrol, 3′-hydroxy-4′-O-methyl grabridine, 4′-O-methyl grabridine, Hispa grabridin B It is preferable to contain a compound. Furthermore, it contains at least one compound selected from the group consisting of glabrizine and glabrene, and also consisting of glabrol, 3′-hydroxy-4′-O-methyl glabridine, 4′-O-methyl glabridine, and Hispa grab lysine B More preferably. In particular, it is most preferable to contain all of grabrene, grabridine, grabrol, 3'-hydroxy-4'-O-methyl grabridine, 4'-O-methyl grabridine, and hispagrabridine B.

  Here, glabridin, 3′-hydroxy-4′-O-methylglabridin, 4′-O-methylglabridin, 4′-O-methylglabridin, Hispaglabridin B is a flavonoid classified into isoflavan, and is a compound represented by the following general formula (1).

[Gravidine is R1 = H, R2 = OH; 3′-hydroxy-4′-O-methylgrabridine is R1 = OH, R2 = OCH ₃ ; 4′-O-methylgrabridine is R1 = H, R2 = OCH ₃ ; In Hispagrabridine B, R1 to R2 are —CH═CH—C (CH ₃ ) ₂ —O— to form a six-membered ring. ]
Glabrene is a flavonoid classified into isoflav-3-ene and is a compound represented by the following general formula (2).

  Glabrol is a flavonoid classified as flavanone, and is a compound represented by the following general formula (3).

  Gravuren, glabrizine, glabrol, 3′-hydroxy-4′-O-methyl glabrizine, 4′-O-methyl glabrizine, and Hispa glabrizine B are components that are specifically contained in Glycyrrhiza glabra among licorice It is almost not included in other varieties. However, G. It may be included in hybrids of grabra and other varieties. The compound can be detected and quantified separately from other flavonoid components by HPLC analysis using a reverse phase column such as ODS.

  It is preferable to use the said compound in the state melt | dissolved in fats and oils. In that case, the purified or synthesized compound may be directly dissolved in oil or fat, but as described later, licorice is extracted with a water-soluble organic solvent to obtain a licorice extract containing the compound, A method of contacting and mixing oils and fats with the extract is simple and preferable.

  By the way, in the present invention, it is important to increase the active ingredient content in the licorice non-aqueous solvent extract and to keep the content of water-soluble impurities such as glycyrrhizin low. Glycyrrhizin is also used as a medicine, but it has a blood pressure-elevating effect, myocardial damage, and wateremia (Harders, H. & Rausch-Stroomann, JG, Munch. Med. Wschr. 95, 580 (1953)), low Potassiumemia, decreased plasma renin activity, pseudoaldosteronism, relaxed limb paralysis (Conn, JW, Rovner, DR & Cohen, EL, J. Am. Med. Assoc. 205, 492 (1968)), urine It has side effects such as decreased aldosterone excretion, edema, headache, lethargy (above, Epstein, MT, et al., Brit. Med. J., 1, 488 (1977)), and 150 times sweeter than sucrose. There is.

  The incorporation of glycyrrhizin inhibits various effects of the licorice non-aqueous solvent extract, and is disadvantageous for use as a food because of the above-mentioned side effects and strong sweetness. In order to increase the active ingredient content in the resulting licorice non-aqueous solvent extract and to keep the water-soluble impurity content such as glycyrrhizin low, it is preferable to reduce the water content coexisting during extraction with the non-aqueous solvent. For that purpose, it is generally important to use licorice that is as dry as possible and to use a non-aqueous solvent that contains as little water as possible. However, licorice is, of course, a plant, and in order to obtain licorice as dry as possible, it is not always sufficient to carry out sundrying or the like that is usually performed, and it is necessary to use a dryer or the like. This is a major difficulty in production on an industrial scale. In addition, even if a non-aqueous solvent that does not contain water is used for the first time, it is difficult to completely prevent the mixing of moisture from the licorice used and the working environment. As recovered. If this is recycled as it is, an extraction solvent with a high water content will be used, so the non-aqueous solvent should be disposable, or a special and expensive purification facility for dehydration will be required. This leads to an increase in manufacturing costs.

  Even when a non-aqueous solvent containing water is used, in order to obtain the above-mentioned high-quality licorice non-aqueous solvent extract, the licorice form has a high proportion of the skin area in the total surface area, usually 30% Above, preferably 50% or more, more preferably 70% or more, particularly 80% or more, and particularly preferably 90% or more. In that case, when the water-soluble organic solvent as described above, preferably a monohydric alcohol having 2 to 4 carbon atoms, more preferably ethanol is used as the non-aqueous solvent, the effect is exhibited to the maximum. The water content of the non-aqueous solvent used is usually about 30% by volume or less, preferably about 20% by volume or less, more preferably about 10% by volume or less, especially about 8% by volume or less. The lower limit is not particularly limited, but is usually about 3% by volume, preferably about 4% by volume from the viewpoint of practicality.

  By the above preferred method, a high-quality licorice non-aqueous solvent extract having a high active ingredient content and a low water-soluble impurity content can be suitably obtained. The glycyrrhizin content in the resulting extract is preferably 0.5% by weight or less, more preferably 0.2% by weight or less, still more preferably less than 0.01% by weight or less than the detection limit (substantially free of glycyrrhizin). ) Can be minimized. In addition, high-quality extracts can be suitably obtained even when using inexpensive water-containing water-soluble organic solvents as extraction solvents without specially drying licorice, thus simplifying the manufacturing process and reducing costs So you can expect a huge advantage.

  The extraction method using the water-soluble organic solvent in this case is not particularly limited, and the extraction method as described above can be used as it is.

  However, the licorice non-aqueous solvent extract obtained by using a water-soluble organic solvent as an extraction solvent, in particular, hardly dissolves in water and general oils, and is easily solidified and colored with the water-soluble organic solvent extract. Since it is known that it is unstable, it is necessary to formulate it in a state that is easy to use and stable. In addition, since an organic solvent is used for extraction, there are many problems to be improved such as high production cost and large environmental burden.

  In order to solve the problem, two kinds of solvents are used as the non-aqueous solvent used for extraction, and the extract obtained by bringing licorice into contact with the water-soluble organic solvent that is the first non-aqueous solvent is further separated. It is preferable to perform the two-stage extraction of contacting the second non-aqueous solvent.

  At this time, fats and oils are preferable as the second non-aqueous solvent used for the second-stage extraction. In particular, by using a specific oil-soluble polyhydric alcohol fatty acid ester as described later as an oil or fat, the above-mentioned object can be suitably achieved, and an oil or fat composition that can be used for any application as it is can be obtained.

  The fat used as the second non-aqueous solvent in the two-stage extraction is preferably one containing a polyhydric alcohol derivative, and further an oil-soluble polyhydric alcohol fatty acid ester is used as the polyhydric alcohol derivative. Oils and fats containing 10% by weight or more are preferred. In particular, when the oil-soluble polyhydric alcohol fatty acid ester content in the fat is 10% by weight or more, the effect of extracting the licorice non-aqueous solvent extract is sufficiently exhibited. The oil-soluble polyhydric alcohol fatty acid ester used in the present invention is not particularly limited as long as it is a fatty acid ester of an alcohol having two or more hydroxyl groups in the same molecule. For example, glycerin, polyglycerin, sugar, sugar alcohol, Examples include fatty acid esters of polyhydric alcohols such as polysorbate.

  Preferred as the polyhydric alcohol fatty acid ester used in the present invention is a medium-chain fatty acid triglyceride or a glycerin fatty acid ester mainly composed of a medium-chain fatty acid triglyceride. The content of medium-chain fatty acid triglycerides in the fat used as the second non-aqueous solvent is preferably about 50% by weight or more, more preferably about 70% by weight or more. The medium-chain fatty acid triglyceride here is not particularly limited as long as the fatty acid having about 6 to 12 carbon atoms is a constituent fatty acid, but the fatty acid has about 8 to 10 carbon atoms. The ratio is preferably about 50% by weight or more, more preferably about 70% by weight or more. Further, a medium-chain fatty acid triglyceride having a specific gravity at about 20 ° C. of about 0.94 to 0.96 and a viscosity at about 20 ° C. of about 23 to 28 cP is more preferable. Moreover, as the medium chain fatty acid triglyceride, any of naturally derived ones and those prepared by transesterification can be used.

  Further, the polyhydric alcohol fatty acid ester used in the present invention may be a fatty acid partial glyceride or a glycerin fatty acid ester mainly composed of a fatty acid partial glyceride. In that case, the content of the partial glyceride in the fat used as the second non-aqueous solvent is preferably about 50% by weight or more, more preferably about 70% by weight or more. The partial glycerides here are diglycerides (1,2-diacylglycerol, 1,3-diacylglycerol) or monoglycerides (1-monoacylglycerol, 2-monoacylglycerol), and any of them may be used. A mixture of the two may be used, and the constituent fatty acids are not particularly limited, but diglycerides containing unsaturated fatty acids and / or medium chain fatty acids as constituent fatty acids are preferred from the viewpoint of processability. In addition, as the partial glyceride, any of those derived from nature, those prepared by transesterification, and the like can be used.

  Furthermore, the fats and oils used in the present invention may be a mixture of the above-mentioned medium chain fatty acid triglycerides and partial glycerides.

  In the present invention, a method for obtaining a composition obtained by extracting licorice in two stages using two kinds of non-aqueous solvents as described above is not particularly limited, but preferably obtained by the first extraction with a water-soluble organic solvent. The obtained licorice organic solvent extract can be obtained by dissolving in an oil or fat as the second non-aqueous solvent. In this case, the licorice organic solvent extract may be in the form of an extract or from which the solvent is removed. The dissolution of the licorice organic solvent extract in the second non-aqueous solvent can be carried out by ordinary operations such as stirring or mixing, but after dissolving the licorice organic solvent extract in fats and oils by operations such as stirring or mixing, filtration is performed. Alternatively, it is desirable to remove impurities insoluble in fats and oils by operations such as centrifugation. In order to increase the extraction efficiency, it is preferable that the mixture is heated to 30 to 100 ° C., more preferably 40 to 90 ° C., and preferably mixed and stirred. In order to prevent deterioration due to heating, mixing is performed under reduced pressure or in a nitrogen stream. More preferably, stirring is performed. The mixing and stirring time is not particularly limited, but is preferably 1 hour or longer, more preferably 1 to 5 hours, and further preferably 1 to 3 hours. Or the method of WO03 / 84556 can also be used conveniently. For example, it is possible to extract from licorice raw material directly with water-soluble organic solvent and oil, but after mixing oil and fat into the extract obtained by extracting licorice with water-soluble organic solvent, remove water-soluble organic solvent Preferably, after mixing the licorice raw material with ethanol and then mixing the licorice raw material with ethanol, the licorice non-aqueous solvent extract obtained by removing unnecessary components is mixed with fats and oils, preferably medium-chain fatty acid triglycerides. Then, it can be obtained by removing ethanol. Furthermore, in this invention, you may add fats and oils with respect to the mixture of these licorice non-aqueous solvent extracts and fats and oils, and may adjust the composition ratio of a licorice non-aqueous solvent extract and fats and oils.

  In general, a hydrophobic licorice non-aqueous solvent extract is unstable in a powder state, and its stability is not improved even when dissolved in an organic solvent such as ethanol. However, the stability can be improved by dissolving the extract in fats and oils by the preferred method described above. Moreover, since a licorice non-aqueous extract is sparingly water-soluble, it can also be expected that its absorbability is improved by using it in a state dissolved in fats and oils. In that case, the properties of the extract are preferably liquid or slurry.

  When the non-aqueous solvent extract of licorice obtained by the above method is used as the composition for preventing or reducing hangover or hangover of the present invention, the form thereof is not particularly limited. For example, hard capsule, soft capsule, tablet, powder In the form of syrups, drinks, etc., it can be used for foods and drinks such as health foods and health functional foods (foods for specified health use, nutritional functional foods), pharmaceuticals or quasi drugs. Oils and fats in which at least one compound selected from the group consisting of grabrene, grabridine, grabrol, 3′-hydroxy-4′-O-methyl grabridine, 4′-O-methyl grabridine, and Hispa grabridin B is dissolved Can be used alone for cooking or for soft capsule preparations. Furthermore, since the mixture of licorice non-aqueous solvent extract and fats and oils obtained by the above preferred method can be freely mixed with oily objects, the physical properties are adjusted by mixing with other fats and oils according to the purpose. It is possible. In this case, it is preferable that the other fats and oils are foods or pharmaceuticals, and the type and use amount thereof are determined in consideration of various conditions such as physical properties and use temperature range required for each product. By adjusting the amount, characteristics such as consistency and melting point can be controlled. For example, vegetable oils such as corn oil, rapeseed oil, Haieru rapeseed oil, soybean oil, olive oil, safflower oil, cottonseed oil, sunflower oil, rice bran oil, palm oil, palm kernel oil, fish oil, beef tallow, pork fat, milk fat, egg yolk oil An oil composition can be obtained by using animal oils such as these, oils and fats that have been subjected to fractionation, hydrogenation, transesterification, etc., or mixed oils thereof. The oil / fat composition thus obtained can be used as liquid oil / fat such as salad oil and frying oil, plastic oil / fat such as margarine and shortening, water-in-oil emulsion, and oil-in-water emulsion. In addition, as fat and oil-containing compositions for eating and drinking produced using these as raw materials, confectionery such as chewing gum, chocolate, candy, jelly, biscuits and crackers, frozen confectionery such as ice cream and ice confectionery, milk beverage, soft drink, Beverages such as energy drinks and beauty drinks, noodles such as udon, Chinese noodles, spaghetti, instant noodles, kneaded products such as strawberries, bamboo rings, and half pieces, seasonings such as dressings, mayonnaise, sauces, bread, ham, soup, various retort foods Various frozen foods are exemplified and can be used for pet food and livestock feed.

  Furthermore, for the purpose of fortification, various vitamins such as vitamins A, D, and E may be added and used together. Various salts as flavoring agents, various flavorings, milk-related substances such as whole milk powder, You may add skim milk powder, fermented milk, milk fat, etc., and may use together. Further, as raw materials other than the above, all of the antioxidants, colorants and the like used in ordinary water-in-oil emulsions and oil-in-water emulsions can be used.

  The composition for preventing or reducing hangover or hangover of the present invention may contain a carrier acceptable for food or drink or medicine, in addition to the non-aqueous solvent extract of licorice. The pharmaceutical carrier can be any inert, organic or inorganic material suitable for oral, enteral, transdermal, subcutaneous, or parenteral administration, including but not limited to water, gelatin, gum arabic, lactose , Microcrystalline cellulose, starch, sodium starch glycolate, calcium hydrogen phosphate, magnesium stearate, talc, colloidal silicon dioxide, and the like. Such compositions may also contain other pharmacologically active pharmaceutical ingredients, and conventional additives such as stabilizers, wetting agents, emulsifying agents, flavoring agents, buffering agents and the like.

  In the present invention, to confirm the effects of prevention and mitigation of hangover and hangover, animals (mouse, rat, etc.) are used to give ethanol and test samples, and to observe behavior and measure ethanol concentration and acetaldehyde concentration in blood. It is possible by The sample may be fed into food or drinking water, or may be forcibly orally administered using a sonde or the like. The effect of prevention of hangover or hangover can be confirmed by devising the administration timing of the test sample. It is also possible to conduct human testing by volunteers.

  The composition of the present invention has an excellent effect of preventing or reducing not only hangover caused by ethanol but also symptom of hangover due to residual acetaldehyde. That is, the composition of the present invention can be used as an agent for preventing or reducing hangover or hangover. The method of ingesting the composition for preventing or reducing hangover or hangover of the present invention (preventative or reducing agent for hangover or hangover) is not particularly limited, but several hours before drinking such as before drinking, during drinking, or after drinking It is effective to take it for several hours after drinking, and it is more preferable to take it at a time of 0 to 2 hours before drinking. The intake route is not particularly limited, but oral administration is simple and preferable. Or, regardless of alcohol consumption, taking it on a daily basis can also help prevent or reduce hangovers and hangovers, and also help prevent alcoholism, liver damage, pancreatitis, etc. Can be expected. The amount of intake is not particularly limited, but in terms of the amount of licorice non-aqueous solvent extract, an amount in the range of 30 to 500 mg / dose or 30 to 500 mg / day is preferable for adults, 50 mg to 300 mg / dose or 50 to 300 mg An amount in the range of / day is more preferred, and an amount in the range of 80 mg to 200 mg / dose or 80 to 200 mg / day is even more preferred.

  EXAMPLES Hereinafter, the present invention will be described more specifically with reference to examples, but the present invention is not limited to these examples.

  49.25 L of ethanol was added to 9.85 kg of Afghan licorice (G. glabra), extracted twice at 45 ° C. for 2 hours, and then concentrated to obtain 4.4 L of a concentrated solution. Of this, 3 L was further concentrated, treated with activated carbon, and further concentrated to obtain 81.2 g of an ethanol solution containing a licorice non-aqueous solvent extract.

  629.2 g of ethanol solution (containing 125.8 g of licorice non-aqueous solvent extract) and medium chain fatty acid triglyceride (MCT) (actor M-2, manufactured by Riken Vitamin Co., Ltd., fatty acid composition C8: C10 = 99: 1) 187.6 g was mixed and stirred for about 1 hour while being kept at about 80 ° C., and then ethanol was removed by concentration under reduced pressure. Insoluble matter was separated by suction filtration, and 45.7 g of MCT was further added to the filtrate after filtration to obtain 297.0 g of MCT solution. 1 g of the obtained MCT solution was dissolved in methanol for HPLC to make a total amount of 100 ml, and this solution was used as a sample for HPLC analysis under the following conditions. As a result, grabrene, grabridine, grabrol, 4 g It turned out that 6.6 mg, 30.8 mg, 12.6 mg, and 3.7 mg of '-O-methyl glabridine are contained, respectively. The content of the licorice non-aqueous solvent extract in the MCT solution was about 30% by weight.

(HPLC analysis conditions)
As the analytical column, J'sphere ODS-H80, 4.6 × 250 mm (YMC) was used at a column temperature of 40 ° C. In the mobile phase, the acetonitrile ratio with respect to the 20 mM phosphoric acid aqueous solution is kept constant at 35% from the start of analysis to 20 min, and is increased at a constant ratio so that it becomes 70% after 75 min after 20 min, and 80% from 75 min to 80 min. The flow rate was 1 ml / min under a constant gradient condition. The injection volume was 20 μl, and the detection wavelength was 254 nm. The retention time of each compound is 40.0 min for grabrene, 50.2 min for grabridine, 58.3 min for grabrol, and 66.8 min for 4′-O-methyl grabridine.

  SD male rats (Charles River) were purchased at 6 weeks of age and acclimated for about 1 week before being used for experiments at 7 weeks of age. Twenty rats were divided into 4 groups of 5 rats so that the body weights were equal. The normal group was administered 3 ml / kg of MCT and 8 ml / kg of distilled water. The control group received 3 ml / kg of MCT and 8 ml / kg of 37.5% ethanol. To the sample low dose administration group, 1 ml / kg of MCT solution containing the licorice non-aqueous solvent extract obtained in Example 1 and 8 ml / kg of 37.5% ethanol were administered. The MCT solution containing the licorice non-aqueous solvent extract obtained in Example 1 was administered to the sample high dose administration group at 3 ml / kg and 37.5% ethanol at 8 ml / kg. The MCT solution (sample) containing MCT or licorice non-aqueous solvent extract was administered 30 minutes before administration of distilled water or ethanol. The state of drunkenness was evaluated based on the state of spontaneous movement of rats between 5 and 10 minutes after ethanol administration.

  As a result, as shown in Table 1, all individuals in the normal group not taking ethanol did not move, but in the control group, an increase in active locomotor activity considered to be the effect of ethanol was significantly observed. In contrast, the amount of exercise decreased by administering an MCT solution containing a licorice non-aqueous solvent extract, and a tendency to suppress the amount of exercise was observed even at a dose of 1 ml / kg. There was a significant difference. This is considered to be a result of the MCT solution containing the licorice non-aqueous solvent extract suppressing the excited state due to ethanol. That is, it was confirmed that the MCT solution containing the licorice non-aqueous solvent extract has an effect of suppressing the drunk state caused by ethanol.

  About the human efficacy of the MCT solution containing the licorice non-aqueous solvent extract, the MCT solution containing the licorice non-aqueous solvent extract obtained in Example 1 for 16 subjects randomly selected for age and sex, The degree of intoxication and hangover when attending a banquet ingesting 300 mg before drinking, and the intoxication when having a banquet without drinking MCT solution containing licorice non-aqueous solvent extract one week later We evaluated by comparing the degree of hangover. At both banquets, the amount of alcoholic beverages was the same, and the following items with subjective symptoms were answered, and the total number of people was counted. The results are shown below.

As shown in Tables 2 and 3, it is clear that ingestion of an MCT solution containing a licorice non-aqueous solvent extract not only relieves the symptoms of hangover but also relieves the symptoms of hangover. became. In particular, the composition of the present invention showed a strong relaxation effect against hangover.

Production of Soft Capsule The MCT solution containing the licorice non-aqueous solvent extract of Example 1 was pressed into a gelatin film using a rotary type soft capsule production device to obtain a soft capsule having an internal volume of 350 mg.

Production of margarine 80 parts by weight of hardened cottonseed oil (trade name: Snowlight, Kaneka Co., Ltd.), 20 parts by weight of MCT solution containing the licorice non-aqueous solvent extract of Example 1, unsalted butter (Yotsuba Dairy Co., Ltd.) Manufactured) 0.2 parts by weight of glycerin monofatty acid ester (trade name: Emergy MS, manufactured by Riken Vitamin Co., Ltd.) and 0.2 parts by weight of lecithin are added to 10 parts by weight, and the oil phase is dissolved by heating at 60 ° C. Prepared.

  While stirring, 15.1 parts by weight of water was added to 84.9 parts by weight of the prepared oil phase, emulsified for 20 minutes, and then cooled and kneaded with a combinator to prepare margarine.

Preparation of concentrated milk After heating 10 parts by weight of MCT solution containing the licorice non-aqueous solvent extract of Example 1 to 70 ° C., 0.1 part by weight of lecithin and 0.1 part by weight of polyglycerin fatty acid ester were sequentially dissolved. An oil phase was prepared.

  An aqueous phase was prepared by dissolving 25 parts by weight of skim milk powder, 0.1 part by weight of glycerin fatty acid ester and 0.1 part by weight of sucrose fatty acid ester in 64.6 parts by weight of water at 60 ° C.

  The prepared aqueous phase and oil phase were pre-emulsified, and then sterilized with a UHT sterilizer at 145 ° C. for 4 seconds. Next, after vacuum cooling, the mixture was homogenized with a homogenizer at a pressure of 10 MPa, and further cooled to 10 ° C. to obtain concentrated milk for processing.

Production of mayonnaise 10 parts by weight of brewed vinegar, 1 part by weight of salt, 0.6 parts by weight of sugar, 0.2 parts by weight of mustard powder and 0.2 parts by weight of sodium glutamate are added to the mixer at 15-20 ° C. The aqueous phase was prepared by stirring and mixing. Then, 10 parts by weight of egg yolk was added to 68 parts by weight of rice white squeezed oil and 10 parts by weight of the MCT solution containing the licorice non-aqueous solvent extract of Example 1, and an emulsion (10 to 15 ° C.) obtained by stirring and emulsifying While adding little by little, the mixture was stirred at 15 to 20 ° C. and pre-emulsified. Subsequently, final emulsification was performed using a colloid mill to obtain mayonnaise.

Claims (13)

A composition for preventing or reducing hangover or hangover containing a non-aqueous solvent extract of licorice as an active ingredient. The composition according to claim 1, wherein the non-aqueous solvent is an organic solvent, an oil or fat, or a mixed solvent thereof. The composition according to claim 1 or 2, wherein the composition is liquid or slurry. The composition of any one of claims 1 to 3, wherein the glycyrrhizin content in the non-aqueous solvent extract of licorice is 0.5% by weight or less. The non-aqueous solvent extract of licorice is obtained by bringing a second non-aqueous solvent into contact with an extract obtained by bringing licorice into contact with a first non-aqueous solvent. The composition of any one of -4. The composition according to claim 5, wherein the first non-aqueous solvent is an organic solvent. The composition according to claim 6, wherein the organic solvent is at least one selected from the group consisting of monohydric alcohols having 1 to 4 carbon atoms, acetone, heptane, hexane, glycerin, fatty acid ester, diethyl ether, cyclohexane and propylene glycol. object. The composition according to any one of claims 5 to 7, wherein the second non-aqueous solvent is an oil or fat. The composition according to claim 8, wherein the fat or oil contains a polyhydric alcohol fatty acid ester. The composition according to claim 9, wherein the polyhydric alcohol fatty acid ester is a glycerin fatty acid ester. The composition according to claim 8, wherein the fat or oil contains 50% by weight or more of medium chain fatty acid triglycerides. The composition according to claim 8, wherein the fat or oil contains 50% by weight or more of a fatty acid partial glyceride. The composition according to any one of claims 1 to 12, which is for use in medicine.