JP2010018558A - Cosmetic composition containing biosurfactant - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a skin preparation for external use containing a hardly dissolvable substance such as a ceramide, CoQ10, carotenoids, etc., being stable, having a high freedom and suitable for the skin preparation for external use, and further a cosmetic composition, quasi-drug composition, medical supply composition, hygienic material composition and medicinal composition containing the above cosmetic composition, and also a cosmetic, quasi-drug, medical material, hygienic material and medicine, containing the above composition. <P>SOLUTION: This cosmetic composition is characterized by containing a biosurfactant and the hardly dissolvable substance, especially the ceramide, coenzyme Q10 and carotenoids in order to provide the skin preparation for external use, containing the hardly dissolvable substance such as the ceramide, CoQ10, etc., being stable and having the high freedom. As the biosurfactant, the biosurfactant in which a sugar alcohol forms a glycoside bond with a hydroxy group at position 1 of a mannose skeleton can be utilized. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention relates to a cosmetic composition containing a sparingly soluble active ingredient using a biosurfactant as a solubilizer, and a cosmetic, quasi drug, wherein the sparingly soluble active ingredient is ceramide, coenzyme Q10, carotenoid, It relates to medical supplies, hygiene supplies, and pharmaceuticals. More specifically, the biosurfactant includes mannosyl erythritol lipid (MEL) or mannosyl alditol lipid (MAL) containing alditols other than erythritol (MAL includes mannosyl mannitol lipid (MML), mannosyl sorbitol lipid (MSL), mannosyl arabitol lipid ( MAraL) and mannosyl ribitol lipid (MRL)).

  Individual aging and the various diseases that accompany this are closely related to the aging of all dividing cells (decrease in division rate and cell function). For example, the skin is composed of epidermal cells, fibroblasts, and extracellular matrices such as elastin and collagen that support these extracellular skin structures. In young skin, moisture retention, flexibility, and elasticity are ensured by maintaining the constancy of the interaction between these skin tissues, and the skin has a firm and glossy appearance and is kept fresh. However, aging, ultraviolet rays, drying, stress, etc. cause deterioration of the function of the extracellular matrix and fibroblasts in particular. As a result, the skin's flexibility and moisturizing function are reduced, and the skin loses its tension and gloss and becomes rough. Aging symptoms such as wrinkles and dullness occur.

  Searching for an activator and an anti-aging agent has been carried out for the purpose of preventing or suppressing aging at the cellular level. For example, connective tissue hydrolysates (Patent Document 1), thymus / spleen-derived water-soluble protein (Patent Document 2), bovine placenta extract (Patent Document 3) and the like are known as animal-derived activators. Examples of plant-derived activators include sesame seeds, sayaks, capsicum, toki, dokudami, bakumondo (patent document 4), almonds, dandelion, elderberry, cucumber, assembly, perch, carrots, carrots, hops, mokuge, and yokoinin (patents) Document 5, Patent Document 6), extracts of Ginger family Turmeric (Patent Document 7), Hanayoriaceae Hanayasuri (Patent Document 8) and the like are known. Some of these are used as activating agents and anti-aging agents in quasi-drugs and cosmetics, but no activating agents or anti-aging agents that exhibit satisfactory effects have been obtained.

  In addition, studies have been made to prevent or cure rough skin. Rough skin refers to dry skin in which exfoliation of keratinocytes is generally observed. Such rough skin is caused by elution of interstitial lipids such as cholesterol, ceramide, fatty acids, etc., and formation of a stratum corneum permeation barrier due to degeneration of keratinocytes caused by ultraviolet rays, detergents, etc., and disruption of proliferation / keratinization balance of epidermal cells Etc. For the purpose of preventing or healing this rough skin, studies have been made on supplying a keratinocyte lipid component or a synthetic keratinocyte lipid similar thereto.

  This stratum corneum intercellular lipid is a lamellar granule that is biosynthesized by cells in the spinous layer and the granule layer, and is released between the cells just below the stratum corneum and extends, taking a lamellar (lamellar) structure. It has spread to. The lamellar granule is composed of glucosylceramide, cholesterol, ceramide, phospholipid, etc., but the horny layer intercellular lipid hardly contains glucosylceramide. That is, glucosylceramide in lamellar granules is hydrolyzed by β-glucocerebrosidase and converted to ceramide. As a result of this ceramide having a lamellar structure, formation of a stratum corneum permeation barrier is improved as a stratum corneum intercellular lipid. Therefore, it is considered to have a barrier function for preventing rough skin. It has been reported that ceramide supplementation is effective for rough skin caused by a cleaning agent, and shows a high effect in improving rough skin (Non-patent Document 1).

As indicated above, ceramide is an important component of the skin and is said to contribute greatly to the skin barrier function. For this reason, it is useful to improve the skin barrier function of skin whose skin barrier function has decreased due to the decrease of ceramide. In addition, the incorporation of ceramide into an external preparation for skin has been studied. However, ceramides have low compatibility with water and oily components, and there are difficulties in formulation such as the amount is limited to a very small amount and crystals are precipitated during storage. did. For this reason, in the external preparation for skin, it can be said that the action of ceramide is not used effectively. As an example of a stable formulation of ceramide, for example, a method of emulsifying using a polyglycerol fatty acid ester (see, for example, Patent Documents 9 and 10) is known. According to this method, ceramide can surely be emulsified and blended stably, but there is a drawback that the state of the finished preparation is limited to a cream state. It can be said that the development of a formulation technology that can be applied to a dosage form that has a good spread like a milky lotion or essence and is excellent in usability has been desired.

As is well known, coenzyme Q10 is widely used in supplements as an anti-aging agent because of its cell activation and antioxidant effects. However, since coenzyme Q10 is prepared in a powder form, it is necessary to uniformly mix and disperse the coenzyme Q10 powder in cosmetics. However, coenzyme Q10 is poorly water-soluble and highly crystalline. It is difficult to obtain a stable aqueous dispersion due to its properties. Therefore, a technique of emulsifying a mixed oil obtained by dissolving Coenzyme Q10 in an oil agent is used. In Patent Documents 11 and 12 mentioned above, liquid paraffin, silicone oil, and the like are used as oil agents for dissolving coenzyme Q10. However, since these oil agents do not have water-absorbing properties themselves, sufficient moisture retention due to oil components is expected. There was a problem that it was difficult to obtain the rough skin improvement effect by coenzyme Q10 satisfactorily.

On the other hand, carotenoids are known to exhibit various physiological actions such as antioxidant action, immunostimulatory action, and anticarcinogenic action in vivo. For this reason, carotenoids are widely used as components of pharmaceuticals, health supplements, and cosmetics, and various base materials for formulating carotenoids as ingredients of foods and cosmetics have been developed.

For example, Patent Document 13 discloses a method for preparing an aqueous solution of a fat-soluble substance by stirring and mixing a fat-soluble substance such as vitamin A, an emulsifier, a sugar alcohol such as sorbitol, and water, followed by high-pressure treatment. It is disclosed. Patent Document 14 discloses a method for producing a skin cosmetic base material by preparing an aqueous liposome solution using an oil-soluble component, phospholipids, polyhydric alcohol and the like, and then freeze-drying the aqueous liposome solution. Is disclosed.

By the way, carotenoids having no vitamin A efficacy (for example, lutein, zeaxanthin, astaxanthin, canthaxanthin, lycopene) may have high physiological effects on the skin, such as skin aging, keratinization, and reduction of wrinkles. Are known. However, since these carotenoids are not substantially dissolved in water, when these carotenoids are blended in cosmetics mainly composed of water, they are not dispersed in water unless a large amount of surfactant is used. Is usually difficult to manufacture. Moreover, even if an aqueous dispersion can be produced, stability and homogeneity are low, such as precipitation occurring over time.

  On the other hand, a biosurfactant is an amphipathic lipid produced outside the cell by microorganisms. In recent years, there has been a lot of interest in these new microorganism-derived materials against the backdrop of increasing production of plant resources due to the development of biotechnology and lowering costs of downstream processes due to advances in separation technology. Mannosyl erythritol lipid (MEL), a kind of biosurfactant, is a natural surfactant produced by yeast, and various physiological actions have been reported (Non-patent Document 1). Recently, mannosyl mannitol lipid (MML) in which erythritol replaces mannitol has been found (Patent Document 15).

MEL includes 4-O-β-D-mannopyranosyl-meso-erythritol structure and 1-O-β-D-mannopyranosyl-meso represented by the following formula (1) as optical isomers of erythritol having a sugar skeleton. -An erythritol structure (the following formula (2)) exists.

In addition to the surface active action, these MELs are used as anti-inflammatory agents and anti-allergic agents (Patent Document 16), as a hair nourishing / hair restorer (Patent Document 17), and have antibacterial action. (Patent Document 18) and surface tension reducing action (Patent Document 19) are known. Moreover, the effect (patent document 20) which MEL acts as a substitute of ceramide and improves rough skin is reported.

  On the other hand, recently, interest in natural materials, such as natural emulsifiers, has increased due to increased safety concerns, and development of emulsified compositions using natural emulsifiers such as lecithin and saponin has been actively carried out (Patent Document 21). ), (Patent Document 22), (Patent Document 23). However, the emulsifying ability of natural emulsifiers is not so strong, and it has been difficult to stably emulsify hardly soluble substances. Compositions using these materials are not satisfactory because crystals tend to precipitate over time, resulting in poor appearance and poor feel.

A technique for obtaining a stable and highly flexible external skin preparation containing ceramide, coenzyme Q10, and carotenoids by coexistence of MEL, ceramide, coenzyme Q10 (hereinafter also referred to as CoQ10) and carotenoids is completely known. There wasn't. In addition, it has not been known at all that MEL, ceramide, CoQ10, and carotenoids form a stable vesicle dispersion. It is not known at all that such a vesicle dispersion system can be applied not only to a cream system but also to a system having a remarkable fluidity such as a lotion and an emulsion system.
JP-A-62-84024 Japanese Patent Laid-Open No. 63-188867 Japanese Patent Laid-Open No. 03-141299 Japanese Patent Laid-Open No. 10-45615 Japanese Patent Laid-Open No. 10-036279 Japanese Patent Laid-Open No. 10-36279 JP 2004-75632 A JP 2005-89375 A JP 2002-114631 A JP 2004-196666 A JP 2004-107262 A JP 2004-99564 A JP 2000-212066 A JP 2005-179313 A JP 2005-104837 A JP 2005-68015 A JP 2003-261424 A JP-A-57-145896 JP-A-61-205450 WO2007 / 060956 JP 62-175414 A JP 5-25535 JP-A-1-176442 Journal of Bioscience and Bioengineering, 94,187 (2002)

The present invention has been made under such circumstances, and provides an external preparation for skin containing a hardly soluble substance such as ceramide, CoQ10, and carotenoids, which is suitable as an external preparation for skin and has a high degree of freedom. It is in. Further, a cosmetic composition containing the cosmetic composition, a quasi-drug composition, a medical product composition, a hygiene product composition, a pharmaceutical composition, and a cosmetic, quasi-drug, medical product, hygiene containing the composition. It is to provide supplies and medicines.

As a result of diligent efforts to achieve the above-mentioned object, the present inventors have sought a topical preparation containing a sparingly soluble substance such as ceramide and CoQ10 having a high degree of freedom and a biosurfactant, in particular, alditol other than MEL and erythritol. Contains MAL (MAL includes mannosyl mannitol lipid (MML), mannosyl sorbitol lipid (MSL), mannosyl arabitol lipid (MAraL), mannosyl ribitol lipid (MRL)), including triacyl MEL and triacyl MAL As a result, the present invention has been completed. That is, the present invention includes the following inventions.
(1)
A cosmetic composition comprising a biosurfactant and a hardly soluble substance.
(2)
2. The cosmetic composition according to 1, wherein the biosurfactant has a mannose skeleton.
(3)
3. The cosmetic composition according to 1 or 2, wherein the biosurfactant is a glycoside bond of a sugar alcohol to the hydroxyl group at the 1-position of the mannose skeleton.
(4)
The biosurfactant having a mannose skeleton was selected from the group consisting of mannosyl erythritol lipid (MEL), mannosyl mannitol lipid (MML), mannosyl sorbitol lipid (MSL), mannosyl arabitol lipid (MAraL) and mannosyl ribitol lipid (MRL) 3. The cosmetic composition according to 3, wherein the cosmetic composition is one or more compounds.
(5)
MEL is mannosyl erythritol lipid A (MEL-A), mannosyl erythritol lipid B (MEL-B), mannosyl erythritol lipid C (MEL-C), mannosyl erythritol lipid D (MEL-D), a triacyl derivative of MEL-A, 5. The cosmetic composition according to 4, wherein the cosmetic composition is at least one compound selected from the group consisting of a triacyl form of MEL-B, a triacyl form of MEL-C, and a triacyl form of MEL-D.
(6)
The cosmetic composition according to any one of 1 to 5, wherein the biosurfactant contains a saturated fatty acid and / or an unsaturated fatty acid.
(7)
The cosmetic composition according to any one of 1 to 6, wherein the biosurfactant forms a lamellar structure and / or a liposome.
(8)
8. The cosmetic composition according to 7, wherein a coexisting active ingredient is embedded in a lamellar structure formed by a biosurfactant and / or a liposome.
(9)
The cosmetic composition according to any one of 1 to 8, wherein the hardly soluble substance is ceramide.
(10)
The cosmetic composition according to any one of 1 to 8, wherein the hardly soluble substance is coenzyme Q10.
(11)
The cosmetic composition according to any one of 1 to 8, wherein the hardly soluble substance is a carotenoid.
(12)
The cosmetic composition according to any one of 1 to 11, which is used as a cosmetic, a quasi-drug, a medical product, a hygiene product, or a pharmaceutical product.

According to the cosmetic composition containing the biosurfactant of the present invention, a stable and highly flexible external preparation for skin containing a hardly soluble substance such as ceramide, CoQ10, carotenoids, etc. can be provided. Cosmetics, quasi-drugs, medical supplies, hygiene supplies Can provide pharmaceuticals. In addition, since biosurfactant is a biologically derived natural surfactant, it is excellent in safety, and the cosmetic composition according to the present invention has an effect that it can sufficiently withstand long-term use. Furthermore, since biosurfactants can be produced by culturing microorganisms, raw material costs are low, and mass production is possible. Therefore, the cosmetic composition according to the present invention has an effect that it can be provided at a low price. The biosurfactant may be used as a premix product.

  A cosmetic composition containing biosurfactant has both cleansing properties, moisturizing effect, collagen production promoting effect, cell activation effect, anti-aging effect, hair growth and skin roughening effect, vesicle formation and liquid crystal form performance, etc. It is also useful as a cosmetic ingredient.

(Biosurfactant)
“Biosurfactant” is a general term for substances having surface-active ability and emulsifying ability produced by living organisms, and not only exhibits excellent surface activity and high biodegradability, but also has various physiological functions. Therefore, there is a possibility of developing different behaviors and functions from synthetic surfactants. Biosurfactants can be produced in large quantities by culturing microorganisms and may be used as premixed products.

  A “premix product” is a product obtained by adding a dispersing agent or the like in addition to a functional material, or diluting with a solvent so that it can be easily used during cosmetic production.

  Biosurfactants include mannosyl erythritol lipid (MEL), mannosyl alditol lipid (MAL) (for MAL, mannosyl mannitol lipid (MML), mannosyl sorbitol lipid (MSL), mannosyl arabitol lipid (MAraL), mannosyl ribitol lipid (MAL). MRL)), trehalose lipids, rhamnolipids, sophorolipids, surfactins, spiculsporic acid, emalzan and the like, and any of them can be used as the activator according to the present invention. Among these, it is preferable to use a biosurfactant that forms a lamellar structure, and MEL and MAL are particularly preferable. These biosurfactants may be either a mixture of fermented products, a purified product or a lamellar structure.

(MEL)
MEL is classified into four types, MEL-A, MEL-B, MEL-C, and MEL-D, based on the presence or absence of acetyl groups at the 4-position and 6-position of mannose. Formula (3) shows the structure of MEL-A. In formula (3), R1 and R2 represent a hydrocarbon group. That is, MEL-A is a compound having an alkanoyl group having 6 to 20 carbon atoms at the 2nd and 3rd positions of mannose and the acetyl group at the 4th and 6th positions of mannose in formula (3). In MEL-B, mannose 4-position acetyl group (CH ₃ CO) in formula (3) is H, and MEL-C is mannose 6-position acetyl group (CH ₃ CO) in formula (3). H and MEL-D is H in the acetyl group (CH ₃ CO) at the 4-position and 6-position of mannose in the formula (3).

[R ¹ and R ² are the same or different and each represents a hydrogen atom or an alkyl group having 1 to 18 carbon atoms, preferably 2 to 16 carbon atoms, more preferably 4 to 12 carbon atoms having a straight chain or a branched chain, or An alkenyl group, alkadienyl group or alkatrienyl group having a straight chain or branched chain having 2 to 18 carbon atoms, preferably 2 to 16 carbon atoms, more preferably 2 to 14 carbon atoms. ]

According to the formula (3), MEL is a saturated or unsaturated straight chain having 1 to 19, preferably 1 to 17, more preferably 1 to 15 carbon atoms at the 2nd and 3rd positions of mannose. Alternatively, it is a compound having a branched alkanoyl group and the mannose 4th and 6th positions are acetyl groups (one of the mannose 4th and 6th acetyl groups, or both are hydroxyl groups). It is good.) The total number of carbon atoms of the 2nd and 3rd alkanoyl groups is preferably 10 to 38, more preferably 14 to 30.

Preferable groups for R ¹ and R ² include C _{7 to} C ₁₁ alkyl groups such as CH ₃ (CH ₂ ) ₆ , CH ₃ (CH ₂ ) ₈ , and CH ₃ (CH ₂ ) ₁₀ . MEL-A of R ^1, R ² is an alkyl group of C ₇ -C ₁₁ Formula (3), myristic acid, palmitic acid, stearic acid, oleic acid or an ester (mono alkyl ester, mono -, di - , Tri-glyceride, or fats and oils containing these saturated or monounsaturated fatty acids) and the like can be obtained by adding them to the medium. Similarly, MEL-A of the formula (3) in which R ¹ and R ² are an alkenyl group, an alkadienyl group or an alkatrienyl group is a linoleic acid, linolenic acid, arachidonic acid having two or more double bonds as a raw material. It can be obtained by adding raw materials such as esters (monoalkyl esters, mono-, di-, tri-glycerides, oils and fats containing these highly unsaturated fatty acids, etc.) to the medium. Preferred alkanoyl groups represented by R ¹ and R ² include C _{7 to} C ₁₁ alkenyl groups such as C ₇ H ₁₄ , C ₉ H ₁₈ , and C ₁₁ H ₂₂ . Preferable alkadienyl groups represented by R ¹ and R ² include C _{7 to} C ₁₁ alkadienyl groups such as C ₇ H ₁₂ , C ₉ H ₁₆ , and C ₁₁ H ₂₀ . Preferable alkatrienyl groups represented by R ¹ and R ² include C _{7 to} C ₁₁ alkatrienyl groups such as C ₇ H ₁₀ , C ₉ H ₁₄ , and C ₁₁ H ₁₈ .

  The structure of MAL other than MEL is shown in Formula (4) (wherein either one or both of the acetyl groups at the 4th and 6th positions of mannose may be hydroxyl groups). Mannitol, arabitol, ribitol, and sorbitol are added as sugar alcohols (alditol) other than erythritol (n = 4: mannitol, sorbitol, n = 2: arabitol, ribitol). According to the formula (4), MAL is a saturated or unsaturated straight chain having 2 to 20 carbon atoms, preferably 4 to 18 carbon atoms, more preferably 6 to 14 carbon atoms at the 2nd and 3rd positions of mannose. Alternatively, it is a compound having a branched alkanoyl group and the mannose 4th and 6th positions are acetyl groups (wherein either one of the mannose 4th and 6th acetyl groups, or both are hydroxyl groups) It may be.)

[R ¹ and R ² are the same or different and each represents a hydrogen atom or an alkyl group having 1 to 18 carbon atoms, preferably 2 to 16 carbon atoms, more preferably 4 to 12 carbon atoms having a straight chain or a branched chain, or An alkenyl group, alkadienyl group or alkatrienyl group having a straight chain or branched chain having 2 to 18 carbon atoms, preferably 2 to 16 carbon atoms, more preferably 2 to 14 carbon atoms. ]

(Triacyl derivative)
The biosurfactant used in the present invention may be MEL triacyl and MAL triacyl other than MEL. The triacyl biosurfactant is a biosurfactant having higher hydrophobicity than MEL or MAL other than MEL. For example, it also exists in the culture solution of MEL-producing bacteria, and when it is obtained in a large amount, it can be produced by reacting MEL with various vegetable oils using enzymes.

  The triacyl form of MEL, that is, triacyl mannosyl erythritol lipid (sometimes referred to as triacyl MEL) has a structure represented by the formula (5).

  In the formula (5), R1, R2 and R3 each independently represent a hydrocarbon group or an oxygen atom-containing hydrocarbon group, and one or both of the hydroxyl groups at the 4-position and 6-position of mannose are acetylated. It may be substituted with a group. The hydrocarbon group may have only a saturated bond or may have an unsaturated bond. When it has an unsaturated bond, it may have a plurality of double bonds. The carbon chain may be linear or branched. In the case of an oxygen atom-containing hydrocarbon group, the number and position of oxygen atoms contained are not limited.

  In the formula (5), R1 and R2 preferably have 1 to 18 carbon atoms, and have an ester bond with the hydroxyl groups at the 2- and 3-positions of mannose as the aliphatic acyl group (RCO-), An acetyl group may be ester-bonded to the hydroxyl group. R3 is preferably an aliphatic acyl group having 2 to 20 carbon atoms, and has an ester bond with the primary hydroxyl group of erythritol as an aliphatic acyl group (RCO-).

  Triacyl MEL has a structure in which a fatty acid ester is added and has high hydrophobicity, and therefore is superior as an emollient in that it can be easily combined with various oil components as compared with conventional MEL.

  These biosurfactants may be used alone, or two or more biosurfactants can be used in combination.

(Biosurfactant production method)
The method for producing the biosurfactant is not particularly limited, and a fermentation method using microorganisms may be arbitrarily selected. For example, culture production of MEL (MEL-A, MEL-B, MEL-C) can be produced by Pseudozyma antarctica NBRC 10736 according to a conventional method, and Pseudozyma antarctica, Pseudozyma sp., Etc. can be used as microorganisms. . It is a well-known fact that an MEL mixture can be easily obtained with any microorganism. The MEL mixture can be purified using silica gel column chromatography to isolate MEL-A, MEL-B and MEL-C. Moreover, Pseudozyma antarctica and Pseudozyma tsukubaensis, Kurzmanomycessp are known as bacteria producing MEL-B, and these bacteria may be used. Pseudozyma hubeiensis and Pseudozyma graminicola are known as bacteria that produce MEL-C, and these bacteria may be used. The microorganism having the ability to produce MEL is not particularly limited, and can be appropriately used depending on the purpose.

  Fermentation media for producing biosurfactants are generally composed of N sources such as yeast extract and peptone, C sources such as glucose and fructose, and inorganic salts such as sodium nitrate, dipotassium hydrogen phosphate and magnesium sulfate heptahydrate. A medium with a typical composition can be used, including oils and fats such as olive oil, soybean oil, sunflower oil, corn oil, canola oil and coconut oil, and water-insoluble substrates such as hydrocarbons such as liquid paraffin and tetradecane. These can be used alone or in combination of two or more.

  Fermentation conditions such as pH and temperature, culture time, and the like can be arbitrarily set, and the culture solution after fermentation can be used as it is as the biosurfactant of the present invention. In addition, it is possible to appropriately add any operation such as filtration, centrifugation, extraction, purification, sterilization and the like to the culture solution after fermentation, and the obtained extract can be diluted, concentrated and dried. .

  As oils and fats used as raw materials, vegetable oils and fats are preferable. Vegetable fats and oils are not particularly limited, and can be appropriately selected according to the purpose. For example, soybean oil, rapeseed oil, corn oil, peanut oil, cottonseed oil, safflower oil, sesame oil, olive oil, palm oil, etc. Among them, soybean oil and olive oil are biosurfactants (especially MEL) production efficiency (production amount) , Production rate, and yield) are particularly preferable. These may be used alone or in combination of two or more.

  There is no restriction | limiting in particular as an inorganic nitrogen source, Although it can select suitably according to the objective, For example, ammonium nitrate, urea, sodium nitrate, ammonium chloride, ammonium sulfate, etc. are mentioned.

  There are no particular limitations on the method for recovering and purifying the biosurfactant, and it can be appropriately selected according to the purpose. For example, it can be recovered by centrifuging the culture solution to recover the oil, and extracting and concentrating with an organic solvent such as ethyl acetate.

  Examples of the extraction solvent include water, alcohols (for example, lower alcohols such as methanol, absolute ethanol, and ethanol, or polyhydric alcohols such as propylene glycol and 1,3-butylene glycol), ketones such as acetone, diethyl ether, and dioxane. , Esters such as acetonitrile and ethyl acetate, and organic solvents such as xylene, benzene, and chloroform can be used alone or in any combination of two or more kinds, and each solvent extract is combined. Can also be used.

  There are no particular limitations on the extraction method, but usually it may be in the range of the boiling point of the solvent from room temperature to normal pressure. After extraction, it is filtered or adsorbed, decolored and purified using an ion exchange resin to form a solution. , Paste, gel, and powder. In many cases, it can be used as it is, but if necessary, further purification treatment such as deodorization and decolorization may be added as long as the effect is not affected. As a purification treatment means such as deodorization and decolorization, an activated carbon column or the like may be used, and a normal means generally applied depending on the extracted substance may be arbitrarily selected. If necessary, a high-purity biosurfactant can be obtained by purification using a silica gel column.

(Triacyl production method)
A method for obtaining a biosurfactant triacyl form will be described by taking a method for producing a MEL triacyl form as an example, but the biosurfactant triacyl form used in the present invention is not limited to a triacyl MEL.

  For example, in order to obtain a triacyl MEL, a triacyl MEL fraction can be purified from a culture solution produced by fermenting a microorganism as described above. In order to obtain a large amount, MEL is dissolved in an organic solvent, a fatty acid derivative such as vegetable oil is added, and esterification or transesterification is performed in the presence of a hydrolase.

  The fatty acid introduced into the erythritol part of MEL may be a long-chain hydrocarbon monovalent carboxylic acid. Further, it may be a saturated fatty acid or an unsaturated fatty acid. In the case of an unsaturated fatty acid, it may have a plurality of double bonds. The carbon chain may be linear or branched. Furthermore, a fatty acid derivative that is a derivative of a fatty acid may be used in the present invention, or a mixture of a fatty acid and a fatty acid derivative may be used in the present invention. The fatty acid or fatty acid derivative introduced into the erythritol part of MEL is preferably derived from oils, higher fatty acids, and synthetic esters.

  The “oils” may be vegetable oils, animal oils, mineral oils, and hardened oils thereof. Specifically, avocado oil, olive oil, sesame oil, camellia oil, evening primrose oil, turtle oil, macadamian nut oil, corn oil, mink oil, rapeseed oil, egg yolk oil, persic oil, wheat germ oil, sasanqua oil, castor oil , Linseed oil, safflower oil, cottonseed oil, eno oil, soybean oil, peanut oil, tea seed oil, kaya oil, rice bran oil, kiri oil, jojoba oil, cacao butter, palm oil, horse oil, palm oil, palm kernel oil Animal and vegetable oils such as beef tallow, sheep fat, pork tallow, lanolin, whale wax, beeswax, carnauba wax, molasses, candelilla wax, squalane and their hardened oils. Examples thereof include mineral oil such as liquid paraffin and petrolatum, and synthetic triglycerin such as glycerin tripalmitate. Preferably avocado oil, olive oil, sesame oil, camellia oil, evening primrose oil, turtle oil, macadamia nut oil, corn oil, mink oil, rapeseed oil, egg yolk oil, persic oil, wheat germ oil, southern oil, castor oil, flaxseed oil , Safflower oil, cottonseed oil, eno oil, soybean oil, peanut oil, tea seed oil, kaya oil, rice bran oil, and more preferably olive oil and soybean oil.

  Examples of the “higher fatty acid” include caproic acid, caprylic acid, capric acid, lauric acid, myristic acid, palmitic acid, oleic acid, linoleic acid, linolenic acid, stearic acid, behenic acid, 12-hydroxystearic acid, isostearic acid, Examples include undecic acid, tolic acid, eicosapentaenoic acid, docosahexaenoic acid, and the like. Preferred are lauric acid, myristic acid, palmitic acid, oleic acid, linoleic acid, linolenic acid, stearic acid and undecylenic acid, and more preferred are oleic acid, linoleic acid and undecylenic acid.

  Examples of the “synthetic ester” include methyl caproate, methyl caprylate, methyl caprate, methyl laurate, methyl myristate, methyl palmitate, methyl oleate, methyl linoleate, methyl linolenate, methyl stearate, undecine. Methyl acid, ethyl caproate, ethyl caprylate, ethyl caprate, ethyl laurate, ethyl myristate, ethyl palmitate, ethyl oleate, ethyl linoleate, ethyl linolenate, ethyl stearate, ethyl undecinate, vinyl caproate , Vinyl caprylate, vinyl caprate, vinyl laurate, vinyl myristate, vinyl palmitate, vinyl oleate, vinyl linoleate, vinyl linolenate, vinyl stearate, vinyl undecinate, cetyl octanoate , Octyldodecyl myristate, isopropyl myristate, myristyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, Oren'i acid decyl dimethyl octanoate, cetyl lactate, myristyl lactate, and the like. Preferred are methyl laurate, methyl myristate, methyl palmitate, methyl oleate, methyl linoleate, methyl linolenate, methyl stearate, methyl undecylate, more preferably methyl oleate, methyl linoleate, and methyl undecylate. .

  Triacyl MEL can be produced by dissolving MEL in an organic solvent and reacting as described above. The organic solvent is not limited as long as it can solubilize MEL. What is necessary is just to be able to solubilize a part even if not all can be solubilized. The organic solvent may be a mixture of a plurality of organic solvents. Specifically, for example, methanol, ethanol, propanol, butanol, acetone, propanone, butanone, pentan-2-one, 1,2-ethanediol, 2,3-butanediol, dioxane, acetonitrile, 2-methyl-butane -2-ol, tertiary butanol, 2-methylpropanol, 4-hydroxy-2-methylpentanone, tetrahydrofuran, hexane, DMF, DMSO, pyridine, methyl ethyl ketone and the like. Acetone, tetrahydrofuran, tertiary butanol, acetonitrile and dioxane are preferable, and acetone is more preferable.

  Examples of hydrolases include lipases, proteases, and esterases. It is preferable to use at least one selected from these, and a plurality of hydrolases may be used. Lipase and esterase are preferable, and lipase is more preferable.

  Specifically, for example, MEL purified from a culture solution of MEL-producing microorganisms is dissolved in an organic solvent (for example, acetone), and commercially available lipase (for example, Novozyme 435 (manufactured by Novozymes) etc.) and vegetable oils and fats are dissolved therein. Added.

  In the case of this production method, the reaction temperature is 10 to 100 ° C., preferably 20 to 50 ° C., more preferably 25 to 40 ° C., and stirring may be performed for 1 to 7 days. Further, molecular sieves may be added to the reaction solution. By this manufacturing method, MEL added as a material becomes a triacyl body almost quantitatively.

  The purification of triacyl MEL can be performed according to the above-described purification of MEL.

(Method for producing MEL having saturated fatty acid side chain)
MEL having a saturated side chain can be produced in accordance with the above-described method for producing biosurfactant. At that time, as the fats and oils used as a raw material, it is carried out by adding one or more water-insoluble substrates such as olive oil, saturated fatty acids or esters thereof, hydrocarbons such as liquid paraffin and tetradecane.
The MEL having a saturated side chain can be purified according to the above-described MEL purification.

(Manufacturing method of MAL such as MML)
The production of MAL having alditol other than erythritol, such as MML, can be carried out according to the above-described method for producing biosurfactant. In that case, as a raw material, it carries out by adding alditol, such as mannitol, arabitol, ribitol, sorbitol, alone or in combination.
Purification of MAL having an alditol other than erythritol such as MML can be performed according to the above-described purification of MEL.

(Method for obtaining liposome)
Biosurfactants may be dissolved in oil-soluble bases or oil-soluble ingredients and blended into cosmetics, and are preferably blended in the form of liposomes in cosmetics, particularly water-based cosmetics such as lotions and moisturizing liquids. . Biosurfactants blended in liposomes are preferable because they are fused with skin cells to improve absorbability. As a method for preparing the liposome, any known preparation method may be adopted, and it is not particularly limited. Examples thereof include an ethanol injection method and a bangham method.

For example, the preparation of the MEL-B liposome solution by the ethanol injection method may be performed as follows. That is, 10 mg of MEL-B is dissolved in 0.5 ml of ethanol, 1 ml of distilled water previously warmed to around 70 ° C. is added and lightly shaken, and then the remaining ethanol is distilled off with a rotary evaporator. This may be sonicated for about 5 minutes using a water bath type ultrasonic device (W-220R, Honda Electronics Co., Ltd.) and then diluted with distilled water.

For example, the preparation of the MEL-B liposome solution by the Bangham method may be performed as follows. That is, 10 mg of MEL-B may be dissolved in 1 ml of chloroform, and the solvent may be distilled off with a rotary evaporator to form a thin film. What is necessary is just to add 1 ml of distilled water there and sonicate for about 5 minutes using a water bath type ultrasonic device (W-220R, Honda Electronics Co., Ltd.).

(Method for producing cosmetic composition containing biosurfactant)
The addition amount of the biosurfactant in the cosmetic composition may be added as long as the biosurfactant does not impair the action of stabilizing the hardly soluble substance, and is usually preferably 0.001 to 50% by mass, and 0.01 to 25% by mass. % Is more preferable, 0.05 to 15% by mass is further preferable, and 0.01 to 10% by mass is particularly preferable. Here, the use form of the biosurfactant added to the cosmetic composition is arbitrary. For example, the biosurfactant may be used as it is as an extract from the culture solution, or after being purified, purified or suspended in water, or dissolved in an organic solvent such as ethanol.

Known ceramides can be used as the ceramides, and any of natural ceramides, synthetic ceramides, and pseudoceramides (synthetic pseudoceramides) can be used.

There are ceramide types 1 to 7 because of their structure, and any of these can be used in the external preparation for skin of the present invention. Among these, ceramide 2 and / or ceramide 3 can be exemplified as a more preferable one, and ceramide 2 is particularly preferable.

Such ceramides are already commercially available as raw materials for external preparations for skin, and such commercial products can be purchased and used in the present invention. Examples of commercially available ceramides as cosmetic ingredients include "Ceramide 1" (Ceramide 1), "Ceramide 2" (Ceramide 2), "Ceramide III" (Ceramide 3), and "Ceramide IIIA" manufactured by Cosmo Farm. (Ceramide 3), “Ceramide IIIB” (Ceramide 3), “Ceramide VI” (Ceramide 6) and “Ceramide TTI-001” (Ceramide 2) manufactured by Takasago International Corporation. Such an ingredient acts as an active ingredient, has an action of improving the skin barrier function and increasing skin water retention, and at the same time, an action of improving the stability of vesicles in the dosage form of the external preparation for skin of the present invention. Have

In order to develop such an action, the lower limit is preferably 0.0005% by mass, more preferably 0.001% by mass, and still more preferably 0.05%, based on the total amount of the external preparation for skin. % By mass. As an upper limit, Preferably, it is 15 mass%, More preferably, it is 10 mass%, More preferably, it is 5 mass%. If the amount is too small, there may be cases where the improvement of the skin barrier function, the skin water retention or the stability of the vesicles cannot be expressed, and if the amount is too large, it will not fit in the vesicles and will precipitate as insoluble matter. This is because there are cases where it occurs. Moreover, considering the blending of ceramide into a skin external preparation from a different viewpoint, ceramide is a highly crystalline substance, and in an ordinary emulsification system, the upper limit of the blending is 0.05% by mass. It is said that the upper limit of the liquid crystal emulsion system of polyglycerin fatty acid ester and polyhydric alcohol which can contain the most ceramide is 0.3% by mass.

CoQ10 is a benzoquinone derivative having an isoprenoid chain composed of 10 isoprene units as a side chain, and is widely distributed in nature. In animals and plants, it is involved in the electron transport system of oxidative phosphorylation in mitochondria.

CoQ10 used in the invention is not particularly limited as long as it can be ingested by a living body, for example, a mammal, especially a human. Examples of the CoQ10 include commonly used industrial synthetic products, cell extracts such as yeast extract obtained by fermentation, sardines, pigs, cows, chickens, broccoli, eggplant, garlic, cabbage, etc. Extracts from animals and plants are included, and each can be used alone or in admixture of two or more. Preferably, it is better to use a yeast extract obtained by fermentation from the viewpoint of product image or production efficiency. In addition, since there are those having an isoprenoid chain consisting of 1 to 12 isoprene units as side chains (CoQ1 to 12) in nature, the CoQ10 composition of the present invention inhibits the expression of the desired effect of the present invention. Unless otherwise specified, analogs having different lengths of their isoprenoid chains may be included.

Coenzyme Q10 is widely used in supplements as an anti-aging agent because of cell activation and antioxidant effects.

In order to express such an effect, the content of CoQ10 is preferably 0.0005% by mass to 15% by mass, more preferably 0.001% by mass to 10% by mass with respect to the total amount of the external preparation for skin. More preferably, it is 0.05 mass%-5 mass%.

Carotenoids are naturally occurring yellow to red terpenoid pigments that can be found in plants, algae, and bacteria. Examples of carotenoids include hydrocarbons (carotenes) and oxidized alcohol derivatives thereof (xanthophylls). As these, actinioelisrol, astaxanthin, bixin, canthaxanthin, capsanthin, capsorbin, β-8′-apo-carotenal (apocarotenal), β-12′-apo′-carotenal, α-carotene, β-carotene, “ Carotenes "(mixtures of [alpha]-and [beta] -carotenes), [gamma] -carotene, [beta] -cryptoxanthin, lutein, lycopene, biolerithrin, zeaxanthin, and esters of those containing hydroxyl or carboxyl. Many of the carotenoids occur naturally in the form of cis and trans isomers, but the composites are often racemic mixtures. Carotenes are generally extracted from plant materials. For example, lutein extracted from marigold petals is widely used as a feed ingredient for poultry, and colors poultry skin and fat and eggs produced by poultry. Many of the carotenes are also produced synthetically. Most commercially available β-carotene is produced synthetically.

Examples of suitable carotenoids include actinioerythrol, astaxanthin, bixin, canthaxanthin, capsanthin, β-8′-apo-carotenal (apocarotenal), β-12′-apo′-carotenal, α-carotene, β -Containing carotene, "carotene" (mixture of α- and β-carotene), γ-carotene, β-cryptoxanthin, lutein (xanthophyll), lycopene, biorelythrin, zeaxanthin, and those containing hydroxyl- or carboxyl- Examples include esters.
In particular, astaxanthin has an antioxidant effect, an anti-inflammatory effect, an anti-skin aging effect, and a whitening effect. Therefore, it has been requested to be added to foods, cosmetics, raw materials for pharmaceuticals and processed products thereof. Considered and implemented.

In addition, astaxanthins (including astaxanthin and esters thereof) are widely distributed in the animal and plant kingdoms in nature, and are mainly used as coloring agents for farmed fish and chickens. Astaxanthin is known to have an antioxidant effect, an anti-inflammatory effect (Patent Document 1), an anti-skin aging effect (Patent Document 2), and a whitening effect (Non-Patent Document 1). Because of these effects, astaxanthin has been studied and implemented in the past for foods, cosmetics, raw materials for pharmaceuticals and processed products thereof.
As described above, astaxanthin is widely distributed in the animal and plant kingdoms in nature and can be obtained as a natural extract extracted from them, but its chemical structure is already known, so organic chemical synthesis It is also obtained by.

The carotenoid content in the cosmetic composition containing a carotenoid such as astaxanthin of the present invention is preferably 0.1 to 10% by mass, more preferably 0.5 to 5% by mass, and still more preferably 0.2 to 2% by mass. It is.

(Method of using cosmetic composition containing biosurfactant)
The form of the cosmetic composition containing the biosurfactant is not particularly limited. Therefore, it may take any state such as solid, liquid, paste, jelly, and powder. In order to form such a state, it can be solidified using, for example, a gelling agent, or can be dispersed using a liquid. Moreover, a solvent can be added to make a solution, or it can be spray-dried to form a powder.

The cosmetic composition containing the biosurfactant of the present invention has an excellent moisturizing effect and skin roughening preventing effect, etc., to maintain a smooth surface by imparting appropriate moisture to the skin surface, and vesicle formation / liquid crystal type performance. It has been confirmed. That is, the cosmetic composition containing the biosurfactant of the present invention can retain moisture of the skin for a long time. An object of the present invention is to provide a preparation for external application to the skin containing a hardly soluble substance such as ceramide, CoQ10 and carotenoids which is stable and highly flexible. Therefore, the cosmetic composition containing the biosurfactant of the present invention can be used very effectively when it is necessary to improve rough skin, improve keratin or protect the skin.

For example, a cosmetic composition containing the biosurfactant of the present invention can be used as a cosmetic. For example, cosmetic soap, shampoo, facial cleanser, rinse, eye cream, eye shadow, cream / milky lotion, lotion, perfume, funny, cosmetic oil, hair cosmetic, hair dye, perfume, powder, pack, cleansing cream, shaving Cream, shaving lotion, suntan oil, sunscreen oil, sunscreen lotion, sunscreen lotion, suntan cream, sunscreen cream, foundation, powdered perfume, blusher, mascara, eyebrow, nail cream, nail enamel, beauty nail enamel removal It can be used as a liquid, hair wash, bath cosmetic, lipstick, lip balm, eyeliner, toothpaste, deodorant agent, eau de cologne, hair nourishing agent and hair restorer. The cosmetic composition containing the biosurfactant of the present invention can also be used as an ointment or a poultice.

Various components such as biosurfactant, whitening agent, anti-aging agent, moisturizer, ultraviolet absorber, antibacterial agent, and the like should be further added to the cosmetic composition containing the biosurfactant of the present invention. Can do.

(Whitening agent)
The whitening agent may be a melanin production inhibitor and / or a tyrosinase activity inhibitor.

For example, L-ascorbic acid and derivatives thereof, hydroquinone and derivatives thereof, lucinol, edetic acid and derivatives thereof, and placenta extract, t-AMCHA, acerola extract, age extract, ellagic acid or derivatives thereof, fire retardant Extract, chamomile extract, chamomile flower extract / (urea), kiwi extract, glutathione, tocotrienol, ferulic acid, raspberry ketone, lucinol, walnut extract, pyridoxine dipalmitate, sulfur, kojic acid or its derivatives, glucosamine or its derivatives, hydroxykehi Acid or its derivatives, glutathione, arnica extract, urgonum extract, scorpion extract, psycho extract, bowfish extract, garlic mycelium culture or extract thereof, linden extract, peach leaf extract, age Examples include tsusu extract, cucumber extract, gills extract, toki extract, oysterin extract, oyster leaf extract, diaper extract, button pi extract, hamamelis extract, maronier extract, hypericum extract, and oil-soluble licorice extract.

These whitening agents can be used alone or in combination of two or more.

(Anti-aging agent)
The anti-aging agent may be an anti-inflammatory agent and / or an antioxidant.

For example, anti-inflammatory agents and antioxidants are used as anti-aging agents. Anti-inflammatory agents include glycyrrhizic acid and its salts, glycyrrhetinic acid and its salts, isopropylaminocaproic acid and its salts, allantoin, lysozyme chloride, guaiazulene, salicylic acid. Examples of methyl, γ-oryzanol, and antioxidants include carotenoids such as α-carotene, β-carotene, γ-carotene, lycopene, cryptoxanthine, lutein, zeaxanthin, isozeaxanthin, rhodoxanthine, capsanthin, and crocetin; 1,4-diazacyclo Octane, 2,5-dimethylfuran, 2-methylfuran, 2,5-diphenylfuran, 1,3-diphenylisobenzofuran, α-tocopherol, β-tocopherol, γ-tocopherol, d-tocopherol, histidine, tryptophan, Thionine, alanine or alkyl esters thereof; dibutylhydroxytoluene, butylhydroxyanisole, ascorbic acid, tannic acid, epicatechin, epicacatechin, epicatechin gallate, tannins such as rucaine, flavonoids such as rutin, and other gallic acids Propyl, astaxanthin, carotene, tocopherol, ascorbic acid, tetrasodium edetate, erythorbic acid, tocopherol acetate, retinol acetate, dibitylhydroxytoluene, ascorbyl stearate, ascorbyl palmitate, methylsilanol dioletocopherol / silicic acid mixture, nicotine Benzyl acid, photosensitizer 401, aspartic acid, adenosine triphosphate 2Na, aminobutyric acid, fennel extract, Dutch carassier , Caffeine, chlorella extract, saffron extract, ginger extract, soy extract, tiso extract, folic acid, retinol, retinol palmitate, inositol, turmeric extract, oryzanol, carotene, carrot extract, wheat ointment extract, senkyu extract, chimney extract, Toki extract, Toki root extract, Dodami extract, Tocopherol, Tocopherol nicotinate, Button extract, Ergocalciferol, Pyridoxine dicaprylate, Batyl alcohol, Glycyrrhetinyl stearate, Glycyrrhizic acid, Glycyrrhetinic acid, Diphenhydramine hydrochloride, Lysozyme chloride, Amino caproic acid, Ganoderma extract , Yokuinin, Merirot extract, Button extract, Toki extract, Toki root extract, Senkyu extract, Gennosho Co-extract, Allantoin, Arnica extract, Arnica flower extract, Ogon extract, Ouren extract, Odorikosou extract, Catfish ear extract, Chamomile extract, Calamine, Kawara mugwort extract, Licorice extract, Guaiazulene, Gardenia extract, Kumazasa extract, 2K glycyrrhizic acid, Stearyl glycyrrhetinate , Gentian extract, black tea extract, comfrey extract, comfrey leaf extract, tocopherol acetate, methyl salicylate, zinc oxide, shikon extract, purple root extract, perilla extract, perilla leaf extract, periwinkle extract, peonies extract, honeysuckle extract, sage extract, seiyou Kizuta extract, Elderberry extract, Achillea millefolium extract, Assembly extract, Sakuhakuhi extract, Tokinsenka extract, Pyridoxine HCl Loquat leaf extract, Tilia cordata extract, peach leaf-fruit extract, cornflower extract, saxifrage extract, mugwort extract, lettuce extract, Roman chamomile extract, burnet extract and Kakaroru, polyamines and the like.

These anti-aging agents can be used individually by 1 type or in combination of 2 or more types.

(Humectant)
The moisturizing agent may be a hydrophilic moisturizing agent and / or a lipophilic moisturizing agent. As the hydrophilic moisturizing agent, amino acids, peptides, proteins, higher alcohols and derivatives thereof, and as the lipophilic moisturizing agent, phospholipid, sugar. Lipids and steroids may be used.

  As moisturizer, sodium pyridonecarboxylate, glycol, glycerin, glucose, maltose, maltitol, sucrose, fructose, xylitol, sorbitol, maltotriose, threitol, erythritol, amylolytic sugar-reducing alcohol, sorbitol, polyhydric alcohols Is ethylene glycol, 1,4-butylene glycol, diglycerin, triglycerin, tetraglycerin, 1,3-butylene glycol, propylene glycol, dipropylene glycol, polyethylene glycol, 1,3-propanediol, other serine, glycine, Threonine, alanine, collagen, hydrolyzed collagen, hydronectin, fibronectin, keratin, elastin, royal jelly, chondroitin sulfate hepa Glycerophospholipid, glyceroglycolipid, sphingophospholipid, sphingoglycolipid, linoleic acid or its esters, eicosapentaenoic acid or its esters, pectin, bifidobacteria fermentation product, lactic acid fermentation product, yeast extract, litchi mycelium Culture or extract thereof, wheat germ oil, avocado oil, rice germ oil, jojoba oil, soybean phospholipid, γ-oryzanol, bellows oyster extract, yokuinin extract, siamese extract, typhoid extract, diatomaceous earth extract, kidachi aloe extract, burdock extract, mannenrou Extract, Arnica extract, wheat bran, pyrrolidone carboxylic acid, tomato extract, camellia oil, soybean phospholipid, hyaluronic acid, threonine, ammonium glycolate, methylsilanol alginate, yocuinine, toki extract, toki root extract , Soybean extract, asparagus extract, DNA-Na, PCA-Na, RNA-Na, ashitaba extract, aspartic acid, amatya extract, alanine, arginine, alginate sodium, altea extract, aloe vera extract, oyster extract, barley extract, oyster leaf, Hydrolyzed keratin, hydrolyzed collagen, hydrolyzed conchiolin, hydrolyzed eggshell membrane, hydrolyzed egg white, hydrolyzed silk, hydrolyzed soy protein, brown algae extract, karin extract, raspberry extract, xylitol, chitosan, cucumber extract, cucumber fruit extract, Guava confectionery extract, quince seed extract, glycine, glycerin, glucose, grapefruit extract, grapefruit fruit extract, clematis extract, burdock extract, rice fermented liquor, chondroitin sulfate Na, fish colageno, sanza Extract, Shiitake extract, Giant extract, Glycerin, Cystine, Cysteine, Horsetail extract, Mallow extract, Serine, Sorbitol, Soy protein, Tomato extract, Lactic acid Na, Lactobacillus, Soybean fermented extract, Urea supine / Novara extract / Almond koji, Corn oil, Honey, hyaluronic acid Na, fukuno extract, betaine, loofah extract, maltitol, maltose, mannitol, lily extract, lactoferrin, lysine, apple extract, lotus extract, royal jelly, lanolin fatty acid polyethylene glycol 1000, lanolin fatty acid isopropyl, isopropyl myristate, myristic acid Isobutyl, hexyldecanol, myristyl lactate, lanolin fatty acid, tricaprylglyceryl, oleyl alcohol, octyldodecyl oleate Decyl oleate, reduced lanolin, octyldodecanol, almond oil, avocado oil, olive oil, oleic acid, orange rough oil, cocoa butter, carrot extract, sesame oil, sasanqua oil, safflower oil, dihydrocholesterol, squalane, cholesteryl stearate , Ceramide 2, evening primrose oil, sunflower oil, sunflower oil, cefnide 3, sunflower seed oil hybrid sunflower oil, phytosphingosine, grape seed oil, jojoba oil, jojoba seed oil, mineral oil, mink oil, macadamia nut oil, meadow foam oil, Examples include yuri oil, eucalyptus leaf oil, lanolin, linoleic acid, rosehip oil, petrolatum, and polyglutamic acid.

These humectants can be used alone or in combination of two or more.

(UV absorber)
As UV absorbers, use benzoic acid UV absorbers, anthranilic acid UV absorbers, salicylic acid UV absorbers, cinnamic acid UV absorbers, triazine UV absorbers, benzophenone UV absorbers, etc. Also good.

For example, as an ultraviolet absorber, amyl benzoate, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylate, benzyl cinnamate, paramethoxycinnamate 2 -Ethoxyethyl, octyl paramethoxycinnamate, glyceryl mono-2-ethyl hexanoate diparamethoxycinnamate, isopropyl paramethoxycinnamate, diisopropyl diisopropyl cinnamate ester, urocanic acid, ethyl urocanate, hydroxymethoxy Benzophenone, hydroxymethoxybenzophenonesulfonic acid and its salts, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone disulfonic acid Sodium, dihydroxybenzophenone, tetrahydroxybenzophenone, 4-tert-butyl-4′-methoxy-dibenzoylmethane, 2,4,6-trianilino-p- (carbo-2′-ethylhexyl-1′-oxy) -1, 3,5-triazine, 2- (2-hydroxy-5-methylphenyl) benzotriazole, 2-ethylhexyl paradimethylaminobenzoate, ethyl paraaminobenzoate, topylmethoxydipenzoylmethane, oxybenzone-1, ethyl guaiazulenesulfonate Examples of zinc oxide, sinoxate, and anthranilic acid-based ultraviolet absorbers include homomenthyl-N-acetylanthranylate.

These ultraviolet absorbers can be used alone or in combination of two or more.

(Antimicrobial agent)
The antibacterial agent may be an organic antibacterial agent and / or an inorganic antibacterial agent.

For example, as an antibacterial agent, buckwheat extract, halocarban, chlorophenesin, lysozyme chloride, alkyldiaminoethylglycine hydrochloride, isopropylmethylphenol, thymol, hexachlorophene, berberine, thioxolone, salicylic acid and their derivatives, benzoic acid, sodium benzoate , Paraoxybenzoic acid ester, parachlormetacresol, benzalkonium chloride, phenoxyethanol, isopropylmethylphenol, carboxylic acid, sorbic acid, potassium sorbate, hexachlorophene, chlorhexidine chloride, trichlorocarbanilide, photosensitizer, bis (2-pyridylthio) -1-oxide) zinc, thianthol, hinokitiol, triclosan, trichlorohydroxydiphenyl ether, chlorhexidine gluconate Phenoxyethanol, resorcin, azulene, salicylic acid, zinc pyrithione, sodium mononitroguayacol, fennel extract, pepper extract, cetylpyridinium chloride, etc. benzethonium chloride and undecylenic acid derivatives.

These antibacterial agents can be used alone or in combination of two or more.

In the cosmetic composition containing the biosurfactant of the present invention, various components other than the biosurfactant, whitening agent, anti-aging agent, moisturizer, ultraviolet absorber, and antibacterial agent should be further added depending on the purpose of use. Can do.

For example, in a cosmetic composition containing a biosurfactant, the biosurfactant is highly hydrophobic, so it is dissolved in a nonionic surfactant, a lower alcohol, a polyhydric alcohol, or a natural oil such as olive oil, squalane, or a fatty acid. May be.

  Nonionic surfactants include, for example, sorbitan fatty acid esters (for example, sorbitan monooleate, sorbitan monoisostearate, sorbitan monolaurate, sorbitan monopalmitate, sorbitan monostearate, sorbitan sesquioleate, sorbitan trioleate). Glycerol, penta-2-ethylhexyl diglycerol sorbitan, tetra-2-ethylhexyl diglycerol sorbitan, etc.); glycerin polyglycerin fatty acids (eg mono cottonseed oil fatty acid glycerin, monoerucic acid glycerin, sesquioleate glycerin, glyceryl monostearate) , Α, α′-oleic acid pyroglutamate glycerin, monostearate glycerin malate, etc.); propylene glycol fatty acid esters (for example, monos Propylene glycol tea acrylate, etc.); hardened castor oil derivative; glycerin alkyl ether and the like.

  Examples of the POE-based hydrophilic nonionic surfactant include POE-sorbitan fatty acid esters (for example, POE-sorbitan monooleate, POE-sorbitan monostearate, POE-sorbitan monooleate, POE-sorbitan tetraoleate). POE sorbite fatty acid esters (eg, POE-sorbite monolaurate, POE-sorbite monooleate, POE-sorbite pentaoleate, POE-sorbite monostearate, etc.); POE-glycerin fatty acid esters (eg, POE-glycerol monostearate, POE-glycerin monoisostearate, POE-monooleate such as POE-glycerol triisostearate, etc.); POE-fatty acid esters (for example, POE-distearate) POE-alkyl ethers (for example, POE-lauryl ether, POE-oleyl ether, POE-stearyl ether, POE-behenyl ether, POE-2-octyldodecyl ether), POE-monodiolate, ethylene glycol distearate, etc. POE-cholestanol ether, etc.); Pluronic type (for example, Pluronic, etc.); POE • POP-alkyl ethers (for example, POE • POP-cetyl ether, POE • POP-2-decyltetradecyl ether, POE • POP−) Monobutyl ether, POE / POP-hydrogenated lanolin, POE / POP-glycerin ether, etc.); tetra-POE / tetra-POP-ethylenediamine condensates (for example, Tetronic, etc.); POE-castor oil-cured castor Oil derivatives (eg POE-castor oil, POE-hardened castor oil, POE-hardened castor oil monoisostearate, POE-hardened castor oil triisostearate, POE-hardened castor oil monopyroglutamic acid monoisostearic acid diester, POE -Hardened castor oil maleic acid, etc.); POE-beeswax lanolin derivatives (eg POE-sorbite beeswax etc.); alkanolamides (eg coconut oil fatty acid diethanolamide, lauric acid monoethanolamide, fatty acid isopropanolamide etc.); POE- Examples thereof include propylene glycol fatty acid ester; POE-alkylamine; POE-fatty acid amide; sucrose fatty acid ester; alkylethoxydimethylamine oxide; trioleyl phosphate.

  Examples of the lower alcohol include ethanol, propanol, isopropanol, isobutyl alcohol, t-butyl alcohol and the like.

  Examples of the polyhydric alcohol include divalent alcohols (for example, ethylene glycol, propylene glycol, trimethylene glycol, 1,2-butylene glycol, 1,3-butylene glycol, tetramethylene glycol, 2,3-butylene glycol, Pentamethylene glycol, 2-butene-1,4-diol, hexylene glycol, octylene glycol, etc.); trivalent alcohol (eg, glycerin, trimethylolpropane, etc.); tetravalent alcohol (eg, 1, 2, 6) Pentaerythritol such as hexanetriol, etc .; pentavalent alcohol (eg, xylitol, etc.); hexavalent alcohol (eg, sorbitol, mannitol, etc.); polyhydric alcohol polymer (eg, diethylene glycol, dipropylene glycol, Tylene glycol, polypropylene glycol, tetraethylene glycol, diglycerin, polyethylene glycol, triglycerin, tetraglycerin, polyglycerin, etc.); divalent alcohol alkyl ethers (for example, ethylene glycol monomethyl ether, ethylene glycol monoethyl ether, ethylene glycol) Monobutyl ether, ethylene glycol monophenyl ether, ethylene glycol monohexyl ether, ethylene glycol mono 2-methylhexyl ether, ethylene glycol isoamyl ether, ethylene glycol benzyl ether, ethylene glycol isopropyl ether, ethylene glycol dimethyl ether, ethylene glycol diethyl ether, ethylene glycol Dibutyl Dihydric alcohol alkyl ethers (for example, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, diethylene glycol monobutyl ether, diethylene glycol dimethyl ether, diethylene glycol diethyl ether, diethylene glycol butyl ether, diethylene glycol methyl ethyl ether, triethylene glycol monomethyl ether, triethylene glycol) Monoethyl ether, propylene glycol monomethyl ether, propylene glycol monoethyl ether, propylene glycol monobutyl ether, propylene glycol isopropyl ether, dipropylene glycol methyl ether, dipropylene glycol ethyl ether, dipropylene glycol Dibutyl alcohol ether ester (for example, ethylene glycol monomethyl ether acetate, ethylene glycol monoethyl ether acetate, ethylene glycol monobutyl ether acetate, ethylene glycol monophenyl ether acetate, ethylene glycol diazebate, ethylene glycol disuccine) , Diethylene glycol monoethyl ether acetate, diethylene glycol monobutyl ether acetate, propylene glycol monomethyl ether acetate, propylene glycol monoethyl ether acetate, propylene glycol monopropyl ether acetate, propylene glycol monophenyl ether acetate, etc.); glycerin monoalkyl ether (example) Sugar alcohol (eg, sorbitol, maltitol, maltotriose, mannitol, sucrose, erythritol, glucose, fructose, starch-degrading sugar, maltose, xylitol, starch degradation) Sugar-reduced alcohol, etc.); Glysolid; Tetrahydrofurfuryl alcohol; POE-Tetrahydrofurfuryl alcohol; POP-Butyl ether; POP / POE-Butyl ether; Tripolyoxypropylene glycerin ether; POP-glycerin ether; POP-glycerin ether phosphate POP · POE-pentane erythritol ether, polyglycerin and the like.

  Oils include avocado oil, olive oil, sesame oil, camellia oil, evening primrose oil, turtle oil, macadamian nut oil, corn oil, mink oil, rapeseed oil, egg yolk oil, persic oil, wheat germ oil, sasanca oil, castor oil , Linseed oil, safflower oil, cottonseed oil, eno oil, soybean oil, peanut oil, tea seed oil, kaya oil, rice bran oil, kiri oil, jojoba oil, cacao butter, palm oil, horse oil, palm oil, palm kernel oil Animal and vegetable oils such as beef tallow, sheep fat, pork tallow, lanolin, whale wax, beeswax, carnauba wax, molasses, candelilla wax, squalane and their hardened oils. There are mineral oils such as liquid paraffin and petrolatum, and synthetic triglycerins such as glycerin tripalmitate.

  Examples of higher fatty acids include lauric acid, myristic acid, palmitic acid, oleic acid, linoleic acid, linolenic acid, stearic acid, behenic acid, 12-hydroxystearic acid, isostearic acid, undecylic acid, tallic acid, eicosapentaenoic acid, docosahexaene. There are acids. Examples of higher alcohols include lauryl alcohol, cetyl alcohol, stearyl alcohol, behenyl alcohol, myristyl alcohol, oleyl alcohol, cetostearyl alcohol, jojoba alcohol, lanolin alcohol, batyl alcohol, 2-decyltetrathececinol, cholesterol, phytosterol, and isostearyl. There is alcohol. Synthetic esters include, for example, cetyl octanoate, octyldodecyl myristate, isopropyl myristate, myristyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, decyl orenate, dimethyl octanoate, cetyl lactate, myristyl lactate, etc. There is. Examples of silicone include linear polysiloxanes such as dimethylpolysiloxane and methylphenylpolysiloxane, cyclic polysiloxanes such as decamethylcyclopolysiloxane, and three-dimensional network structures such as silicone resins.

  As described above, the cosmetic composition containing the biosurfactant and the hardly soluble substance according to the present invention is preferably combined with a quasi-drug, a medical product, a hygiene product, and a pharmaceutical product.

  When the cosmetic composition comprising the biosurfactant and the hardly soluble substance according to the present invention is implemented in the form of a quasi-drug, medical product, hygiene product, or pharmaceutical product, the dosage form is not limited, and the ampule , Capsules, powders, granules, pills, tablets, solids, solutions, gels, bubbles, emulsions, creams, ointments, sheets, mousses, bath preparations, and the like.

  Specifically, quasi-drugs, medical supplies, sanitary products, and pharmaceuticals include basic cosmetics such as internal and external pharmaceutical preparations, lotions, emulsions, creams, ointments, lotions, oils, packs, and facial cleansing agents. And skin cleansers, shampoos, rinses, hair treatments, hair creams, pomades, hair sprays, hair styling agents, permanents, hair nickings, hair dyes, hair growth and hair restorations, foundations, white powder, white hair, lipstick, Makeup cosmetics such as blusher, eyeshadow, eyeliner, mascara, eyebrows, eyelashes, cosmetics for finishing such as nail care, perfumes, bath preparations, toothpastes, mouth fresheners, gargles, liquid odors -Examples include deodorants, sanitary products, sanitary cotton, and wet tissue. More specifically, cosmetic soap, shampoo, facial cleanser, rinse, eye cream, eye shadow, cream / milky lotion, lotion, perfume, funny, cosmetic oil, hair cosmetics, hair dye, paste perfume, powder, pack, Cleansing cream, shaving cream, shave lotion, suntan oil, sunscreen oil, sunscreen lotion, sunscreen lotion, sunscreen cream, sunscreen cream, foundation, powdered perfume, blusher, mascara, eyebrow, nail cream, beauty nail enamel , Nail enamel remover, hair wash, cosmetics for bath, lipstick, lip balm, eyeliner, toothpaste, deodorant agent, cologne, hair nourishing agent and hair restorer. The cosmetic composition containing the biosurfactant of the present invention can also be used as an ointment or a poultice.

  The cosmetic composition characterized by containing the biosurfactant and the hardly soluble substance according to the present invention is suitably applied to humans. However, as long as each effect can be expected, the cosmetic composition is applied to animals other than humans. It can also be applied to.

  A cosmetic composition comprising the biosurfactant according to the present invention and a hardly soluble substance is included in the scope of the quasi-drug, medical product, hygiene product, pharmaceutical product, as long as the effects of the present invention are not impaired as required. Ingredients and additives used in the above can be used in combination.

  Examples of waxes include beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax, montan wax, shellac wax, and the like.

  Examples of mineral oils include liquid paraffin, petrolatum, paraffin, ozokelide, ceresin, microcristan wax, polyethylene powder, squalene, squalane, and pristane.

For example, fatty acids include lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, tall oil,
Examples include natural fatty acids such as lanolin fatty acid, and synthetic fatty acids such as isononanoic acid, caproic acid, 2-ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2-ethylhexanoic acid, and isopentanoic acid.

  For example, as alcohols, natural alcohols such as ethanol, isopropylanol, lauryl alcohol, cetanol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, phytosterol, synthetic alcohols such as 2-hexyldecanol, isostearyl alcohol, 2-octyldodecanol, etc. , Ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol, polypropylene glycol, 1,3-butylene Glycol, glycerin Batyl alcohol, pentaerythritol, sorbitol, mannitol, glucose, polyhydric alcohols such as sucrose and the like.

  For example, esters include isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyldodecyl myristate, hexyl decyl dimethyloctanoate, cetyl lactate, lactic acid Examples include myristyl, diethyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate, and the like.

  For example, examples of the metal soap include aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, and zinc undecylate.

  For example, gum and water-soluble polymer compounds include gum arabic, benzoin gum, danmar gum, guaiac oil, Irish moss, Karaya gum, tragacanth gum, carob gum, quinseed, agar, casein, dextrin, gelatin, pectin, starch, carrageenan, Carboxyalkylchitin, chitosan, hydroxyalkylchitin, low molecular chitosan, chitosan salt, sulfated chitin, phosphorylated chitin, alginic acid and its salt, hyaluronic acid and its salt, chondroitin sulfate, heparin, ethylcellulose, methylcellulose, carboxymethylcellulose, carboxyethylcellulose , Sodium carboxyethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, nitrocellulose, crystalline cellulose Scan, polyvinyl alcohol, polyvinyl methyl ether, polyvinyl pyrrolidone, polyvinyl methacrylate, polyacrylic acid salts, polyalkylene oxide or crosslinked polymer thereof such as polyethylene oxide or polypropylene oxide, carboxyvinyl polymers, such as polyethyleneimine.

  For example, as the surfactant, anionic surfactant (carboxylate, sulfonate, sulfate ester salt, phosphate ester salt), cationic surfactant (amine salt, quaternary ammonium salt), amphoteric surfactant ( Carboxylic acid type amphoteric surfactant, sulfate ester type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate ester type amphoteric surfactant), nonionic surfactant (ether type nonionic surfactant, ether ester) Type nonionic surfactant, ester type nonionic surfactant, block polymer type nonionic surfactant, nitrogen-containing type nonionic surfactant), other surfactants (natural surfactants, protein hydrolysates) Derivatives, polymer surfactants, surfactants containing titanium and silicon, fluorocarbon surfactants, and the like.

  For example, vitamins such as retinol, retinal (vitamin A1), dehydroretinal (vitamin A2), carotene, lycopene (provitamin A), vitamin B group, thiamine hydrochloride, thiamine sulfate (vitamin B1) ), Riboflavin (vitamin B2), pyridoxine (vitamin B6), cyanocobalamin (vitamin B12), folic acid, nicotinic acids, pantothenic acids, biotins, choline, inositols, vitamin C, ascorbic acid and its derivatives, vitamin D In the group, ergocalciferol (vitamin D2), cholecalciferol (vitamin D3), dihydrotaxosterol, in vitamin E group, tocopherol and its derivatives, ubiquinones, in vitamin K group, phytonadione (vitamin K1), Naquinone (vitamin K2), menadione (vitamin K3), menadiol (vitamin K4) and the like.

  For example, amino acids include valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid, hydroxylysine , Arginine, ornithine, histidine, and the like, and sulfates, phosphates, nitrates, citrates, and amino acid derivatives such as pyrrolidone carboxylic acid.

  For example, as an extract or extract of an animal or plant, herbal medicine, Asenyaku (Asen Yaku), Ashitaba, Acerola, Altea, Arnica, Avocado, Achacha (Amacha), Aloe, Aloe Vera, Nettle, Ginkgo (Ginkgo leaf, Ginkgo), Fennel (cage), turmeric (red gold), usba saishin (spicy), Japanese apricot (salmon plum), Japanese white beetle, walrus, green rose (fruit), hikikoshi (life-extending grass), Japanese oak (yellow cocoon), Koganebana (Ogon), Yamazakura ( Cherry bark), yellowfin, yellow orchid, ginseng, carrot, helicopter, licorice, holland garashi, orange, himehagi (distant), eel-brush (summer hay), tsurugokudami (what head-knot), enju (槐花）, mugwort (guy leaf), scallops (莪 朮), kudu (kazune), kanoko Cormorant (Valgarine), Chamomile, Kikarasuuri (Gurone), Kawara-momogi (Cervus chinensis), Licorice (licorice), Fukidan Popo (Cavity Winter Flower, Clover Winter Leaves), Raspberry, Kiwi Fruit, Japanese Clover (Kikkyo), Chrysanthemum (Chrysanthemum Flower) ), Catalpa (fruit), Citrus fruit (fruit), Tachibana (tachibana skin), Cucumber, Udo or Shishido (fruitful, solitary), apricot, wolfberry (bone crust, coconut, coconut leaf) , Clara, bittersweet, Kumazasa, grapefruit fruit, Nikkei (Kei-Kai), Keigai (Kai-Gai), Ebisugua (Keiko), Maruba morning glory or morning glory (Ken cow), safflower (Safflower), Gobaishi (Pyper), Confrey , Copaiba, gardenia, gentian, honoki (shock), hinata kozuchi (cow knee), goshuyu (burdock), burdock, kosen Garbage, rice, rice bran, wheat, Mishima Saiko (Saiko), saffron, savonso, hawthorn (Yamazako), salamander, salvia, sanchinin ginseng (37 ginseng), shiitake (shiitake), Jiou (geo yellow), Sikunshi (messenger), Murasaki (purple root), perilla (purple leaves, purple soko), oysters (柿蒂), peonies (glaze), psyllium (car forerunner, car front grass), ginger (ginger), Shobu (Japanese cypress), Japanese horned potato (Sadako), Shimotsuke, Birch, Honeysuckle (Gin silver flower, Shinobu), Western ivy, Achillea millefolium, Elderberry, Redwood, Elderberry (Bone), Zeniaoi, River Kyu ), Assembly (this medicine), mulberry (mulberry white skin, mulberry leaf), jujube (daegu), soybean, taranoki, chiketsu carrot (bamboo ginseng), Eggplant (Welcome mother), Warmoko (earth), Dokudami (10 medicines), Fuyumushinatsusatake (Cycium caterpillar), Pepper, Japanese physalis (Torone), Tachikakuso, Ryokucha (green tea), Kocha (tea), Choji (chome) ), Citrus unshiu (Chen bark), camellia, camellia, capsicum (Sanban), Touki (Tochigi), red bellflower, Daidai (Orange bark), Warmoko (dianthus), corn (Southern fur), Tochu (Tochu, Tochu) Leaf), tomato, Nanten (Southern fruit), garlic (large sun), barley (malt), hakusen (white birch), janohi (barley winter), parsley, grasshopper, mint (light load), hamamelis, rose, loquat Leaves (bamboo leaves), Matsuhodo (bamboo leopard), grapes or their leaves, loofah, bodaiju, buttons (peony skin), hops, maikai (my buds), pine Leaves, Marronnier, Mannenrou, Mukuroji, Melissa, Merirot, Bokeh (Kiso), Palms, Peach (Momojin, Peach), Hagi (Giraffe), Binroju (Mariko), Mejijiki (Masame), Cornflower, Yukinoshita ), Bayberry (Yellow Plum), Yashabushi (Yagari), pearl barley (Yokuinin), mugwort, sagebrush, lavender, apple fruit, garlic mushroom (reishi), lemon fruit, forsythia (ream), forsythia, ginkgo biloba Hasidorokoro (roth root), chicken tosaka, bovine / human placenta extract, pig / cow stomach, duodenum, or intestinal extract or its degradation product, water-soluble collagen, water-soluble collagen derivative, collagen hydrolyzate, elastin , Elastin hydrolyzate, water-soluble elastin derivative, silk protein, silk protein Hydrolyzate, and the like bovine hemocyte protein hydrolyzate.

  Examples of microbial culture metabolites include yeast extract, zinc-containing yeast extract, germanium-containing yeast extract, selenium-containing yeast extract, magnesium-containing yeast extract, rice fermentation extract, Euglena extract, and lactic acid fermented milk powder. .

  For example, examples of the α-hydroxy acid include glycolic acid, citric acid, malic acid, tartaric acid, and lactic acid.

  For example, inorganic pigments include anhydrous silicic acid, magnesium silicate, talc, kaolin, bentonite, mica, titanium mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, calcium carbonate, magnesium carbonate, yellow oxide Examples include iron, bengara, black iron oxide, gunjo, chromium oxide, chromium hydroxide, carbon black, and calamine.

  For example, astringents include lactic acid, tartaric acid, succinic acid, citric acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc p-phenolsulfonate, potassium aluminum sulfate, resorcin, ferric chloride, tannic acid Etc.

  Examples of the bactericidal / disinfectant include acrinol, sulfur, benzalkonium chloride, benzethonium chloride, methyl rosaniline chloride, cresol, calcium gluconate, chlorhexidine gluconate, sulfamine, mercurochrome, lactoferrin or a hydrolyzate thereof.

  For example, selenium disulfide, alkylisoquinolinium bromide, zinc pyrithione, biphenamine, thianthol, castari tincture, ginger tincture, pepper tincture, quinine hydrochloride, strong ammonia water, potassium bromate, sodium bromate And thioglycolic acid.

  For example, as perfumes, natural animal perfumes such as musk, civet, castorium, ambergris, anise essential oil, angelica essential oil, Iran essential oil, Iris essential oil, fennel essential oil, orange essential oil, cananga essential oil, caraway essential oil, cardamom essential oil, guayakwood essential oil , Cumin essential oil, black letter essential oil, cinnamon essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, coriandel essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil, jasmine essential oil, gingergrass essential oil, cedar essential oil, spearmint essential oil, western peppermint essential oil , Otsuka perfume essential oil, tuberose essential oil, clove essential oil, orange flower essential oil, winter green essential oil, trout balsam essential oil, buttery essential oil, rose essential oil, palmarosa essential oil, persimmon essential oil, hiba essential oil, sandalwood essential oil, petitgren essential oil, bay essential oil, vetiva essential oil, bell Vegetable fragrances such as Mott essential oil, Peruvian balsam essential oil, Boad Rose essential oil, melamine essential oil, mandarin essential oil, eucalyptus essential oil, lime essential oil, lavender essential oil, linaloe essential oil, lemongrass essential oil, lemon essential oil, rosemary essential oil, Japanese seed mint essential oil, Other synthetic fragrances can be mentioned.

  For example, as a dye / colorant, a red cabbage dye, a red rice dye, an akane dye, an anato dye, an squid dye, a turmeric dye, an enju dye, a krill dye, an amber dye, a caramel, a gold, a silver, a gardenia dye, a corn dye, Examples include onion pigment, tamarind pigment, spirulina pigment, buckwheat whole plant pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple potato pigment, purple yam pigment, lac pigment and rutin.

  Examples of sweeteners include sugar, sweet tea, fructose, arabinose, galactose, xylose, mannose, maltose, honey, glucose, miraculin, monelin and the like.

  For example, examples of the nutrient fortifier include shell calcined calcium, cyanocolabamine, yeast, wheat germ, soybean germ, egg yolk powder, hemicellulose, heme iron and the like.

  Others, hormones, sequestering agents, pH adjusters, chelating agents, antiseptic / antibacterial agents, refreshing agents, stabilizers, emulsifiers, animal / plant proteins and their degradation products, animal / plant polysaccharides and their Degradation product, animal / plant glycoprotein and its degradation product, blood flow promoter, anti-inflammatory agent / antiallergic agent, cell activator, keratolytic agent, wound treatment agent, foam enhancer, thickener, oral preparation, Deodorizing / deodorizing agents, bittering agents, seasonings, enzymes and the like.

The cosmetic composition containing the biosurfactant of the present invention can stably solubilize poorly soluble substances such as ceramide, coenzyme Q10 and carotenoids. The amount used is determined within a range where the effects of ceramide, coenzyme Q10, carotenoids and the like are sufficiently exerted.

  It should be noted that the specific embodiments and the following examples made in the section of the best mode for carrying out the invention are intended to clarify the technical contents of the present invention, and to such specific examples. It is not to be construed as limiting in any way whatsoever, and those skilled in the art can implement the invention within the spirit of the invention and the scope of the appended claims.

  The following examples further illustrate the present invention in detail but are not to be construed to limit the scope thereof.

Example 1 Production of MEL-A and Triacyl MEL-A Inoculum culture was performed by inoculating P. antarctica NBRC 10736 into a seed medium (20 ml / 500 ml Sakaguchi flask × 4). Cultured overnight at 30 ° C. The obtained culture broth was used as an inoculum. The composition of the seed medium was 4% Glucose, 0.3% NaNO ₃ , 0.02% MgSO ₄ .7H ₂ O, 0.02% KH ₂ PO ₄ , and 0.1% yeast extract. For the culture, 75 ml of the above inoculum was inoculated into 1.5 L (5 L-jar) of the production medium and cultured using 5 L-jar under the conditions of 30 ° C., 300 rpm (stirring rotation), 0.5 L / min (Air). The composition of the production medium was 3% soybean oil, 0.02% MgSO4 · 7H ₂ O, 0.02% KH ₂ PO ₄ and 0.1% yeast extract. 250 ml of the culture solution was centrifuged (6500 rpm, 30 min), the supernatant was removed, and the precipitate (bacteria) was collected. 50 ml of ethyl acetate was added to the precipitate, and after sufficient stirring, the mixture was centrifuged (8500 rpm, 30 min), separated into a precipitate and a supernatant, and the supernatant was concentrated with an evaporator. Using silica gel, chloroform: acetone was eluted with 100: 0 300 ml, 90:10 150 ml, 50:50 600 ml, 30:70 200 ml, 0: 100 200 ml to obtain MEL-A and triacyl MEL-A fractions. .

Example 2 Preparation of MEL-B and triacyl MEL-B
0.2 ml of P. tsukubaensis NBRC1940 was inoculated into a 20 ml seed medium / 500 ml Sakaguchi flask and cultured overnight at 26 ° C. and 180 rpm to obtain a seed fungus. The composition of the seed medium was 4% Glucose, 0.3% NaNO ₃ , 0.02% MgSO 4 · 7H ₂ O, 0.02% KH ₂ PO ₄ , and 0.1% yeast extract. 20 ml of the inoculum was inoculated into 2 L of production medium / 5 L Jar and cultured at 26 ° C., 300 rpm (1/4 VVM, 0.5 L air / min) for 8 days. The culture was centrifuged at 7,900 rpm for 60 min at 4 ° C. to separate the cells (including MEL-B) and the supernatant. 80 ml of ethyl acetate was added to each bacterial cell fraction, and the mixture was stirred up and down so that the bacterial cells were sufficiently suspended, and then centrifuged at 4,900 rpm for 30 minutes at 4 ° C. An equal amount of saturated saline was added to the obtained supernatant and stirred to obtain an ethyl acetate layer. An appropriate amount of anhydrous sodium sulfate was added to the ethyl acetate layer and the mixture was allowed to settle for 30 minutes and then evaporated to obtain a crude MEL-B product. The obtained MEL-B crude purified product was eluted with hexane: acetone using a silica gel column to obtain MEL-B and triacyl MEL-B fraction purified products.

Example 3 Preparation of MEL-C and triacyl MEL-C
0.2 ml of P. hubeiensis frozen stock was inoculated into a 20 ml seed medium / 500 ml Sakaguchi flask and cultured overnight at 26 ° C. and 180 rpm to form a seed fungus. The composition of the seed medium was 4% Glucose, 0.3% NaNO ₃ , 0.02% MgSO 4 · 7H ₂ O, 0.02% KH ₂ PO ₄ , and 0.1% yeast extract. 20 ml of the inoculum was inoculated into 2 L of production medium / 5 L Jar and cultured at 26 ° C., 300 rpm (1/4 VVM, 0.5 L air / min) for 8 days. The culture was centrifuged at 7,900 rpm for 60 min at 4 ° C. to separate the cells (including MEL-C) and the supernatant. 80 ml of ethyl acetate was added to each bacterial cell fraction, and the mixture was stirred up and down so that the bacterial cells were sufficiently suspended, and then centrifuged at 4,900 rpm for 30 minutes at 4 ° C. An equal amount of saturated saline was added to the obtained supernatant and stirred to obtain an ethyl acetate layer. An appropriate amount of anhydrous sodium sulfate was added to the ethyl acetate layer and the mixture was allowed to settle for 30 minutes and then evaporated to obtain a crude MEL-B product. The obtained MEL-C crude product was eluted with hexane: acetone using a silica gel column to obtain MEL-C and triacyl MEL-C fraction purified products.

Example 4
In the production of MEL in Example 1, soybean oil was used as a raw material for production. Instead, olive oil was used and cultured in the same manner as in Example 1 to isolate MEL-A, MEL-B, and MEL-C. Purify. The MEL fraction obtained at this time is called MEL-A (OL), MEL-B (OL), and MEL-C (OL) in order to distinguish from the MEL of Example 1.

Example 5 Mannosyl Mannitol Lipid (MML) Production Pseudozymaparantarctica JCM 11752 strain was cultured. That is, Pseudozyma parantarctica JCM 11752 strain preserved in a preservation medium (malt extract 3 g / L, yeast extract 3 g / L, peptone 5 g / L glucose 10 g / L, agar 30 g / L) In a test tube containing 2 mL of liquid medium having a composition of glucose 20 g / L, yeast extract 1 g / L, sodium nitrate 3 g / L, potassium dihydrogen phosphate 0.3 g / L, and magnesium sulfate 0.3 g / L. One platinum loop was inoculated and cultured with shaking at 30 ° C. for 1 day. Subsequently, the obtained bacterial cell culture solution was mixed with olive oil 50 g / L as a vegetable oil, mannitol 100 g / L as a sugar alcohol, yeast extract 1 g / L, sodium nitrate 3 g / L, potassium dihydrogen phosphate 0.3 g / L. And a Sakaguchi flask containing 30 mL of a liquid medium having a composition of magnesium sulfate 0.3 g / L, and cultured at 34 ° C. for 7 days. The above culture broth was collected, the mannosyl alditol lipids in the broth were extracted with ethyl acetate, purified by silica gel chromatography, and the structure of the product was analyzed by ¹³ C NMR and ¹ H NMR.

Example 6 Production of mannosyl arabitol lipid (MAraL) MAraL was produced by culturing in the same manner as in Example 5 except that culture in Example 5 was carried out in a medium containing 100 g / L of arabitol as a sugar alcohol.

Example 7 Production of Mannosyl Sorbitol Lipid (MSL) MSL was produced by culturing in the same manner as in Example 5 except that the culture of Example 5 was carried out in a medium containing 100 g / L of sorbitol as a sugar alcohol.

Example 8 Production of mannosyl ribitol lipid (MRL) MRL was produced by culturing in the same manner as in Example 5 except that cultivation of Example 5 was carried out in a medium containing 100 g / L of ribitol as a sugar alcohol.

Example 9 Formulation Test and Performance Test Using the biosurfactants obtained in Examples 1 to 8 with the raw materials and blending ratios shown in Tables 1, 2, 3, and 4, respectively, a cosmetic composition was prepared as a prototype. .

Table 5 shows a comparative example in which a cosmetic composition to which no biosurfactant is added is produced as a comparative example. The formulations in Tables 1, 2, 3, 4, and 5 are expressed in mass%, and “-” means no formulation.

Specifically, in both the Examples and Comparative Examples, first, each of the A phase raw materials and the B phase raw materials are separately mixed, and the A phase is heated to 70 ° C. and dissolved and mixed. The above solution was added and heated to 50 ° C. to emulsify with a homomixer to prepare a cosmetic composition.

<Feeling of familiarity with skin>
The panel (evaluator) was allowed to use 5 people, the feeling of use was scored as follows, and the total was obtained as an evaluation score. Based on this, the following criteria were used.
《Points》
2 points: Refreshed at the time of application, and feels familiar after application.
1 point: There is stickiness at the time of application, and there is a feeling of familiarity after application.
0 point: There is a sticky feeling both during coating and after coating.
<Criteria>
◎: Sum 10 to 9 points ... Very good ○: Sum 8 to 7 points… Good △: Sum 6 to 5 points… Good ×: Sum 4 or less… Bad <Stability over time>
Stability over time The stability over time was determined visually for those stored at 50 ° C. for 1 month, and was determined according to the following criteria.
◎: No change in appearance ○: Some change in appearance, but no oil separation, water separation, precipitation, etc. Δ: Some change in appearance, oil separation, water separation, precipitation Things that happened
×: Appearance changed significantly and oil separation, water separation, precipitation, etc. occurred

(Stability test results)
After returning to 20 ° C. after completion of the storage test, Formulation Examples 1 to 23 to which MEL was added dispersed uniformly after shaking, and it was confirmed that there was no deterioration in quality after storage at 50 ° C. Moreover, Comparative Examples 1-3 was isolate | separated and precipitation of one part crystal | crystallization was also recognized. From this, it was clarified that the external preparation for skin of the present invention can stably contain poorly soluble substances such as ceramide, coenzyme Q10, and astaxanthin.

(Useability test results)
As a result of comparing the usability of Formulation Examples 1 to 23 to which MEL was added and Comparative Examples 1 to 3, it became clear that the familiarity to the skin was improved by adding MEL.

It is confirmed that the cosmetic composition containing the biosurfactant of the present invention can stably solubilize poorly soluble substances such as ramid, coenzyme Q10, and carotenoids at any mixing ratio shown in Tables 1, 2, 3, and 4. It was done.

The cosmetic composition characterized by containing the biosurfactant of the present invention and a hardly soluble substance can provide a skin preparation for skin containing a hardly soluble substance such as ceramide, CoQ10 and carotenoids, which is stable and highly flexible. . Further, a cosmetic composition containing the cosmetic composition, a quasi-drug composition, a medical product composition, a hygiene product composition, a pharmaceutical composition, and a cosmetic, quasi-drug, medical product, hygiene containing the composition. It is to provide supplies and medicines. Furthermore, it is expected that the raw material cost will be low, mass production is possible, it can withstand long-term use sufficiently, and biosurfactant has a biodegradability and will contribute greatly to the industry. Is done.

Claims (12)

  A cosmetic composition comprising a biosurfactant and a hardly soluble substance.   The cosmetic composition according to claim 1, wherein the biosurfactant has a mannose skeleton.   The cosmetic composition according to claim 1 or 2, wherein the biosurfactant has a sugar alcohol linked to a glycoside bond to the hydroxyl group at the 1-position of the mannose skeleton. The biosurfactant having a mannose skeleton was selected from the group consisting of mannosyl erythritol lipid (MEL), mannosyl mannitol lipid (MML), mannosyl sorbitol lipid (MSL), mannosyl arabitol lipid (MAraL) and mannosyl ribitol lipid (MRL) 4. Cosmetic composition according to claim 3, characterized in that it is one or more compounds. MEL is mannosyl erythritol lipid A (MEL-A), mannosyl erythritol lipid B (MEL-B), mannosyl erythritol lipid C (MEL-C), mannosyl erythritol lipid D (MEL-D), a triacyl derivative of MEL-A, The cosmetic composition according to claim 4, wherein the cosmetic composition is at least one compound selected from the group consisting of a triacyl form of MEL-B, a triacyl form of MEL-C, and a triacyl form of MEL-D. The cosmetic composition according to any one of claims 1 to 5, wherein the biosurfactant contains a saturated fatty acid and / or an unsaturated fatty acid. The cosmetic composition according to any one of claims 1 to 6, wherein the biosurfactant forms a lamellar structure and / or a liposome. The cosmetic composition according to claim 7, wherein a coexisting active ingredient is embedded in a lamellar structure and / or liposome formed by a biosurfactant. The cosmetic composition according to any one of claims 1 to 8, wherein the hardly soluble substance is ceramide. The cosmetic composition according to any one of claims 1 to 8, wherein the hardly soluble substance is coenzyme Q10. The cosmetic composition according to any one of claims 1 to 8, wherein the hardly soluble substance is a carotenoid. The cosmetic composition according to any one of claims 1 to 11, which is used as a cosmetic, a quasi-drug, a medical product, a hygiene product, or a pharmaceutical product.