JP2010011866A - Wet treatment system, decomposition method of slightly soluble protein, jellyfish-decomposing composition and jellyfish decomposition method using the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a wet treatment system quickly and easily decomposing or the like the subjects such as waste, including slightly soluble proteins such as jellyfish protein, by consuming physicochemical energies such as pressure, chemicals, high temperature and the like as little as possible, and easy in relocation, maintenance management and the like. <P>SOLUTION: The wet treatment system (jellyfish decomposing system) comprises at least a first treatment vessel (decomposing vessel 1 and partition vessel 2) receiving subjects to be treated (jellyfish protein) and processing them in contact with a flown-in liquid material (jellyfish decomposing composition), and a first liquid material transfer means (siphon tube 3) which transfers the liquid material in the decomposing vessel 1 outside when the liquid material flowed in the decomposing vessel 1 reaches a predetermined amount, and preferably has a second treatment vessel (circulation and heating vessel 5) receiving and processing the liquid material transferred outside with the siphon tube 3, and a second liquid transfer means (piping) for transferring the liquid material in the circulation and heating vessel 5 into the decomposition vessel 1. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention can quickly and easily decompose an object to be treated such as waste containing a hardly soluble protein such as a jellyfish protein without consuming physicochemical energy such as pressure, chemicals, and high heat as much as possible. The present invention relates to a wet processing apparatus, a method for decomposing hardly soluble proteins such as jellyfish protein, a composition for decomposing jellyfish, and a method for decomposing jellyfish.

  Jellyfish floating in seawater often occur mainly during warm periods, causing various damage. For example, Echizen jellyfish, which occur in large quantities in the seas near Japan, appear on the coasts of various regions along the ocean current, and in recent years have caused tremendous damage to fisheries such as coastal net fishing. In addition, in coastal plant facilities such as power plants and steelworks that require a large amount of cooling water, seawater intake is provided to use seawater as cooling water, but the jellyfish block the intake. The damage caused by this, that is, the intake water is restricted or stopped and the operation is hindered.

  Conventionally, for the purpose of preventing the jellyfish from entering the intake port, the jellyfish is forcibly moved using air bubbling, a water flow propeller, a ship, etc. and controlled to the intake port in order to prevent the jellyfish from entering the intake port. The first measures to install a net and the second measures to dispose of jellyfish by using a rotary dust remover, pump, etc. have been taken.

  However, in the case of the first countermeasure, jellyfish may enter from the gaps in the control net. In addition, since the jellyfish is discharged while alive, there is a risk of further occurrence of jellyfish. In addition, because fouling organisms such as blue mussels adhere to the control net, it is necessary to clean and expand the control net. During the work, the control net cannot be used, and the control net Drying requires a lot of labor, and it is also necessary to secure a place, and there is a problem that maintenance and management of the control net requires a great deal of cost.

  On the other hand, in the case of the second countermeasure, there is a problem that it is necessary to dispose of the jellyfish that has been landed, which requires a great deal of labor and cost. Specifically, when the jellyfish is sun-dried and disposed of after dehydration and desolubilization, the amount of the jellyfish disposed of is easily affected by the weather, and the sun-drying requires several days. The large amount of landed jellyfish requires a vast place to dry in the sun at once, and there is a problem that it has a great impact on the surrounding environment, such as the generation of rot odors. Further, when the jellyfish is incinerated, there is a problem that it is extremely difficult to incinerate the jellyfish itself because the jellyfish individual is about 95-98% by mass of water. Further, when the jellyfish is decomposed physicochemically using pressure or chemicals (see Patent Documents 1 to 5), it requires a great deal of heat and electric energy for the decomposition treatment, and the equipment of the processing apparatus There is a problem that the cost, construction cost, maintenance cost, etc. become large. On the other hand, when the jellyfish is chemically decomposed with acid or alkali, there is a problem that neutralization is required after the decomposition treatment. On the other hand, when the jellyfish is biologically decomposed using microorganisms or the like, there are the following problems.

  That is, for example, by utilizing a jellyfish degrading enzyme secreted by bacteria belonging to the genus Vibrio or Bacillus, collagen fibers that are proteins forming the jellyfish body are decomposed to remove water in the jellyfish body and reduce the volume. A method is known (see Patent Documents 6 to 9). However, in this case, it takes about one day to decompose the jellyfish, cannot be rapidly decomposed, and some bacteria of the genus Vibrio and Bacillus have pathogenicity. There are problems such as the generation of off-flavors.

  Therefore, there is no effective countermeasure against damage caused by the jellyfish in the seaside plant facility, and the jellyfish is quickly and easily decomposed without consuming physicochemical energy such as pressure, chemicals, and high heat as much as possible. The development of related technology that can do this is desired, but at present.

JP 2001-300505 A JP 2000-5738 A Japanese Patent Laid-Open No. 11-244833 JP 2000-145196 A JP 2001-198566 A JP 2003-53303 A JP-A-11-179327 JP 2001-95564 A JP 2002-136852 A

  An object of the present invention is to solve the conventional problems and achieve the following objects. That is, the present invention quickly and easily decomposes an object to be treated such as waste containing a hardly soluble protein such as jellyfish protein without consuming physicochemical energy such as pressure, chemicals, and high heat as much as possible. It is easy to relocate and maintain, etc. Wet treatment equipment suitable as a jellyfish decomposing equipment, etc., and poorly soluble proteins such as jellyfish protein, physicochemical energy such as pressure, chemicals, high heat etc. as much as possible Provided are a method for degrading a hardly soluble protein that can be rapidly and easily degraded without consumption, a composition for degrading jellyfish suitable for efficient degrading jellyfish, and an efficient degrading method for jellyfish using the same. For the purpose.

As a result of the present inventors diligently studying in order to solve the above-mentioned problems, when a specific contact treatment is performed on an object to be treated such as a hardly soluble protein (particularly jellyfish protein) using a liquid material. The inventors have found that decomposition can be performed very efficiently, and have been completed. Means for solving the above-described problems are as follows. That is,
<1> A first processing container that contains an object to be processed and is subjected to contact processing with the flowing liquid material, and the first processing container when the liquid material flowing into the first processing container reaches a predetermined amount It is a wet processing apparatus characterized by having at least a first liquid material transfer means for transferring the liquid material inside.
In the wet processing apparatus, the first processing container accommodates the object to be processed and performs contact processing with the flowing liquid material. At this time, the to-be-processed object is contact-treated with the liquid material in a state where it is subjected to a fluid pressure by the liquid material flowing into the first processing container. When the liquid material contains a component such as an enzyme that decomposes the object to be treated, the object to be treated is decomposed or the like during the contact treatment. The first liquid material transfer means transfers the liquid material that has flowed into the first processing container and has reached a predetermined amount to the outside. At this time, since the liquid material disappears in the first processing container, the object to be processed falls by its own weight, collides with the bottom surface of the first processing container, and is damaged by an impact or the like at that time. Equalize into small pieces. And if this is repeated as needed, the said to-be-processed target object will be decomposed | disassembled completely or to a desired grade.
<2> A second processing container for storing the liquid material transferred to the outside by the first liquid material transferring means, and a second processing container for transferring the liquid material in the second processing container into the first processing container. It is a wet processing apparatus as described in said <1> which further has a 2nd liquid substance transfer means.
In the wet processing apparatus, the second processing container accommodates the liquid material transferred to the outside by the first liquid material transfer means. The second liquid material transfer means transfers the liquid material in the second processing container into the first processing container. As a result, the liquid object contained in the object to be processed is decomposed or the like and flows into the first process container, and the object to be processed existing in the first process container by the liquid object. Is contact-treated again. The liquid material after the contact treatment contains the object to be treated at a higher concentration.
<3> The wet processing apparatus according to any one of <1> to <2>, wherein the object to be processed is a hardly degradable protein. In the wet processing apparatus, the first processing container contains the hardly decomposable protein and contacts with the inflowing liquid material. At this time, the hardly decomposable protein is contact-treated with the liquid material in a state of receiving a fluid pressure by the liquid material flowing into the first processing container. When the liquid material contains a component such as an enzyme that degrades the hardly decomposable protein, the hardly degradable protein is decomposed during the contact treatment. The first liquid material transfer means transfers the liquid material that has flowed into the first processing container and has reached a predetermined amount to the outside. At this time, since the liquid substance disappears in the first processing container, the hardly decomposable protein falls by its own weight, collides with the bottom surface of the first processing container, and is damaged by an impact or the like at that time. Equalize into small pieces. And if this is repeated as needed, the said hardly decomposable protein will be decomposed | disassembled completely or to a desired grade.
<4> The wet processing apparatus according to <3>, wherein the hardly degradable protein is a jellyfish protein. In the wet processing apparatus, the first processing container accommodates the jellyfish protein and makes a contact treatment with the flowing liquid material. At this time, the jellyfish protein is contact-treated with the liquid material in a state of receiving a fluid pressure by the liquid material flowing into the first processing container. If the liquid material contains a component such as an enzyme that decomposes the jellyfish protein, the jellyfish protein is decomposed during the contact treatment. The first liquid material transfer means transfers the liquid material that has flowed into the first processing container and has reached a predetermined amount to the outside. At this time, since the liquid substance does not exist in the first processing container, the jellyfish protein falls by its own weight, collides with the bottom surface of the first processing container, and is damaged by an impact or the like at that time. Small pieces. And if this is repeated as needed, the said jellyfish protein will be decomposed | disassembled completely or to a desired grade.
<5> The wet processing apparatus according to any one of <1> to <4>, wherein the first processing container moves the object to be processed upward and causes the liquid material to flow from below. . In the wet processing apparatus, the first processing container accommodates the object to be processed and performs contact processing with the liquid material flowing from the lower part. At this time, the object to be processed is contact-treated so as to be pushed upward by the liquid material flowing into the inside from the lower part of the first processing container. That is, the object to be processed is about to fall in the direction of gravity, while the liquid material comes into contact with the object to be processed that is about to fall. For this reason, due to the synergistic effect of gravity added to the object to be treated and the fluid pressure of the liquid material, the contact treatment is effectively performed, and the object to be treated is completely or to a desired level. It is decomposed efficiently.
<6> The first processing container has a liquid distribution inlet through which the liquid material flows and a liquid distribution outlet through which the liquid material flows out,
<1> to <5>, comprising an inner tank that accommodates an object to be treated and prevents the object to be treated from blocking at least one of the liquid flow inlet and the liquid flow outlet. It is a wet processing apparatus.
In the wet processing apparatus, even when the object to be processed having a diameter larger than the diameter of the liquid flow inlet and the liquid flow outlet in the first processing container is processed, the object to be processed is While it has a diameter larger than the diameter of the liquid flow inlet and the liquid flow outlet, it is trapped in the inner tank. As a result, the processing object does not cause a problem such as interruption of processing due to the liquid flow inlet and the liquid flow outlet in the first processing container being blocked, and the processing efficiency is excellent.
<7> The wet processing apparatus according to <6>, wherein the inner tank is a casing having a mesh having a diameter smaller than the diameter of the liquid distribution inlet and the liquid distribution outlet. In the wet processing apparatus, even when the object to be processed having a diameter larger than the diameter of the liquid flow inlet and the liquid flow outlet in the first processing container is processed, the object to be processed is While having a diameter larger than the diameters of the liquid flow inlet and the liquid flow outlet, the mesh of the frame cannot be passed through, so that it is trapped in the case. As a result, the processing object does not cause a problem such as interruption of processing due to the liquid flow inlet and the liquid flow outlet in the first processing container being blocked, and the processing efficiency is excellent.
<8> The wet processing apparatus according to any one of <1> to <7>, wherein the contact treatment is at least one of a decomposition treatment and an extraction treatment. In the wet processing apparatus, the decomposition treatment as the contact treatment is performed by appropriately selecting the type of the liquid material, the component to be added to the liquid material, and the like according to the type and purpose of the object to be treated. Alternatively, an extraction process is performed.
<9> The wet processing apparatus according to any one of <1> to <8>, wherein the liquid material includes at least one of a decomposing enzyme of the target object and an extraction solvent. In the wet processing apparatus, since the liquid material contains the decomposing enzyme and / or extraction solvent of the object to be processed, the decomposition processing and / or extraction processing as the contact processing is performed.
<10> The wet processing apparatus according to any one of <1> to <9>, wherein the first liquid material transfer unit transfers the liquid material in the first processing container based on a siphon principle. In the wet processing apparatus, the first liquid material transfer means transfers the liquid material in the first processing container according to the principle of the siphon, and uses mechanical and powerful means such as a pump. In addition, the liquid material can be transferred easily and efficiently at low cost.
<11> The wet processing apparatus according to any one of <1> to <10>, wherein the second processing container circulates while the liquid material transferred by the first liquid material transfer unit is heated inside. is there. In the wet processing apparatus, since the second processing container circulates while heating the liquid material inside, the small piece of the object to be processed contained in the liquid material is further decomposed or the like. And uniformly dissolved or dispersed in the liquid.
<12> A jellyfish decomposition apparatus, wherein the object to be treated is a jellyfish protein, and the liquid material includes at least one of a jellyfish protein degrading enzyme and a microorganism that produces the degrading enzyme. It is the wet processing apparatus in any one. In the jellyfish decomposing apparatus as the wet processing apparatus, the first processing container accommodates the jellyfish protein and makes a contact treatment with the flowing liquid substance. At this time, the jellyfish protein is contact-treated with the liquid material in a state of receiving a fluid pressure by the liquid material flowing into the first processing container. And since the said liquid substance contains the jellyfish protein degrading enzyme, the said jellyfish protein is decomposed | disassembled efficiently in the said contact process. The first liquid material transfer means transfers the liquid material that has flowed into the first processing container and has reached a predetermined amount to the outside. At this time, since the liquid substance does not exist in the first processing container, the jellyfish protein falls by its own weight, collides with the bottom surface of the first processing container, and is damaged by an impact or the like at that time. Small pieces. And if this is repeated as needed, the said jellyfish protein will be decomposed | disassembled completely or to a desired grade.
<13> The wet processing apparatus according to <12>, wherein the jellyfish protein degrading enzyme is a protease produced by actinomycetes. In the wet processing apparatus, the jellyfish degrading enzyme is a protease produced by the actinomycetes, so that the jellyfish protein is degraded very efficiently.
<14> The wet processing apparatus according to <13>, wherein the actinomycete is selected from Streptomyces actinomycetes.
<15> The wet processing apparatus according to <14>, wherein Streptomyces genus actinomycetes is selected from sp.
<16> The SP-type actinomycetes is a wet processing apparatus according to the above <15>, selected from 99-GP-2D-5 strain (FERM P-19336) and a derivative strain thereof.
<17> The wet processing apparatus according to any one of <1> to <16>, which is portable. Since the wet processing apparatus can be moved, the processing can be performed by moving to a desired place only when necessary. In particular, when the wet processing apparatus is the jellyfish decomposing apparatus or the like, the jellyfish that is the object to be processed does not always occur, it occurs seasonally, and it may occur suddenly. Since the wet processing apparatus is portable, it is advantageous in terms of installation location and the like.
<18> A jellyfish-decomposing composition comprising a protease produced by actinomycetes. Since the jellyfish decomposing composition contains proteases produced by actinomycetes, the jellyfish protein of the jellyfish is decomposed very efficiently when the jellyfish decomposing treatment is performed using the jellyfish decomposing composition. .
<19> The jellyfish-decomposing composition according to <18>, wherein the actinomycetes is selected from Streptomyces actinomycetes.
<20> The jellyfish-decomposing composition according to <19>, wherein Streptomyces genus actinomycetes is selected from sp. Actinomycetes.
<21> The jellyfish-decomposing composition according to <20>, wherein the SP species actinomycetes are selected from a 99-GP-2D-5 strain (FERM P-19336) and a derivative strain thereof.
<22> A method for decomposing a jellyfish, comprising subjecting the jellyfish to a jellyfish decomposing composition according to any one of <18> to <21>. In the method for decomposing jellyfish, since the specific composition for decomposing jellyfish is used, the jellyfish is efficiently decomposed.
<23> A contact treatment step in which a liquid material containing a degradable protein degrading enzyme is introduced from the lower part of the first treatment container into the first treatment container to be contact-treated, A heating and circulating step of transferring the liquid material that has reached a predetermined amount in the first processing container to the second processing container and circulating while heating in the second processing container; and the second processing A method for degrading a hardly decomposable protein, comprising a liquid material circulation step of transferring and circulating a liquid material in a container into the first processing container.
In the method for degrading a hardly degradable protein, in the contact treatment step, the hardly degradable protein contained in the first treatment container is allowed to flow into the first treatment container. The contact treatment is performed with the liquid containing the decomposing enzyme. In the heating and circulation step, the liquid material whose volume has increased and reached a predetermined amount by flowing into the first processing container is transferred to the second processing container and heated there. Circulated. As a result, the hardly degradable protein and the small pieces thereof contained in the liquid are decomposed more completely and uniformly. Then, when these steps are repeated as necessary, the hardly degradable protein is completely decomposed to a desired level.
<24> The method for degrading a hardly degradable protein according to <17>, wherein the hardly degradable protein is at least one selected from perchloric acid soluble protein (PSP), abnormal prion protein, and jellyfish protein. . In the degradation method of the hardly degradable protein, at least one selected from the perchloric acid soluble protein (PSP), the abnormal prion protein, and the jellyfish protein is efficiently degraded.
<25> The method for degrading a hardly degradable protein according to any one of <23> to <24>, wherein the liquid substance containing a degrading enzyme of the hardly degradable protein contains a protease produced by actinomycetes. In the method for degrading the hardly degradable protein, since the liquid material contains a protease produced by actinomycetes, the hardly degradable protein is extremely efficiently degraded.
<26> The method for degrading a hardly degradable protein according to <25>, wherein the actinomycete is selected from Streptomyces actinomycetes.
<27> The method for degrading a hardly degradable protein according to <26>, wherein the Streptomyces genus actinomycetes is selected from sp.
<28> The method for degrading a hardly degradable protein according to <27>, wherein the SP species actinomycetes are selected from the 99-GP-2D-5 strain (FERM P-19336) and a derivative strain thereof.

  According to the present invention, conventional problems can be solved, and the object to be treated such as waste containing a hardly soluble protein such as jellyfish protein is consumed as much as possible by physicochemical energy such as pressure, chemicals and high heat. Can be quickly and easily decomposed, and can be easily transferred, maintained, etc., and is suitable for a wet processing apparatus suitable as a jellyfish decomposing apparatus, etc., and a hardly soluble protein such as a jellyfish protein, A method for degrading a hardly soluble protein that can be rapidly and easily decomposed without consuming physicochemical energy such as high heat as much as possible, a jellyfish decomposing composition suitable for efficient decomposing jellyfish, and efficiency using the same An effective method of jellyfish decomposition.

FIG. 1 is a schematic explanatory view showing an embodiment of a jellyfish decomposition apparatus which is an aspect of the wet processing apparatus of the present invention.

(Wet processing equipment)
The wet processing apparatus of the present invention includes at least a first processing container and a first liquid material transfer means, and preferably includes a second processing container and a second liquid material transfer means. In addition, other means appropriately selected as necessary are provided.

-First processing vessel-
The first processing container is not particularly limited as long as it is a container that accommodates an object to be processed and can be contacted with an inflowing liquid material, and can be appropriately selected according to the purpose. For example, there may be provided a liquid distribution inlet through which the liquid material flows in and a liquid distribution outlet through which the liquid material flows out, so that the object to be processed is moved upward. It is preferable that the liquid material can be introduced from the lower part, and specifically, the liquid substance having the liquid flow inlet at the lower part is preferable.
In the case of this preferred embodiment, the object to be treated is contact-treated so as to be pushed upward by the liquid material flowing into the liquid flow inlet provided in the lower portion of the first processing container. . That is, the object to be treated is about to fall in the direction of gravity, while the liquid material is directed upward from the liquid flow inlet provided at the lower part of the first processing container. Therefore, the target object to be dropped is brought into contact with the object to be pushed up. For this reason, the contact treatment can be effectively performed by the synergistic effect of the gravity added to the object to be treated and the fluid pressure of the liquid material, and the object to be treated can be completely or desired. It can be efficiently decomposed to the extent.

  There is no restriction | limiting in particular about the material of the said 1st processing container, a shape, a structure, a magnitude | size, a color, etc., According to the objective, it can select suitably. As the material, for example, a material excellent in mechanical strength, durability, chemical resistance and the like is preferable, and can be appropriately selected from known resin materials, metal materials, ceramic materials, and the like. As said shape, a cylindrical shape etc. are mentioned suitably, for example. The structure may be a single tank structure or a composite tank structure of two or more tanks, but the target object to be accommodated in the first processing container is the liquid flow inlet and From the viewpoint of preventing the liquid distribution outlet from being blocked, the composite tank structure is preferable, the object to be processed is accommodated, and at least one of the liquid distribution inlet and the liquid distribution outlet is provided. A composite tank structure including an inner tank that prevents the object to be processed from being blocked is preferable. The inner tank is not particularly limited and may be appropriately selected according to the purpose. For example, a casing having a mesh having a diameter smaller than the diameter of the liquid flow inlet and the liquid flow outlet. Are preferable. The size can be appropriately selected according to the size of the object to be processed, the processing amount, and the like. There is no restriction | limiting in particular as said color, According to the objective, it can select suitably, It is preferable that it is transparent at the point which can see the process condition visually.

-First liquid material transfer means-
As the first liquid material transfer means, as long as the liquid material flowing into the first processing container reaches a predetermined amount, the liquid material in the first processing container can be transferred to the outside. There is no limitation, and it can be selected as appropriate according to the purpose. For example, it is preferable that one end of which is connected to the liquid flow outlet and the liquid in the first processing vessel can be transferred according to the principle of siphon. The principle of the siphon when the amount of the liquid material connected to the liquid flow outlet provided at the lower part of the first processing container and flowing into the first processing container reaches a predetermined amount Therefore, it is particularly preferable that the liquid material in the first processing container can be transferred to the outside from the liquid flow outlet.
As a specific example of such a first liquid material transfer means, a tubular structure having both ends open, the one end of which is provided at the liquid flow outlet provided at the lower part of the first processing container. Connected so as not to flow out, bends substantially vertically and upward in the vicinity of the one end, and in the first processing container at any height position lower than the maximum height of the liquid material accommodated therein, Preferably, the end is bent in the direction in which gravity is applied and in the direction facing the second processing container. In the case of such a first liquid material transfer means, the principle of the siphon is used as compared with the case where a pump or the like is used, so that driving energy is not required, the structure is simple, and maintenance is not required. It is advantageous in that the liquid material and the object to be treated can be contacted until the liquid material reaches a predetermined amount in the first processing container.

-Second processing vessel-
The second processing container is not particularly limited as long as it is a container capable of storing the liquid material transferred to the outside by the first liquid material transfer means, and its material, shape, structure, and size are not limited. The color and the like can be appropriately selected according to the purpose.
As said material, the thing excellent in mechanical strength, heat resistance, chemical-resistance, etc. is preferable, for example, resin, ceramics, such as glass, a metal, etc. are mentioned. As said shape thru | or structure, the shape like a well-known water tank etc. are mentioned, for example. The size can be appropriately selected according to the amount of processing. As said color, transparency is mentioned suitably, for example.

  There is no restriction | limiting in particular as an installation place of said 2nd processing container, Although it can select suitably according to the objective, For example, it accommodates in said 1st processing container by said 1st liquid substance transfer means. From the viewpoint of efficiently transferring the liquid material that has been disposed into the second processing container, it is preferably at a lower position than the first processing container, and an open end in the first liquid material transporting means It is more preferable that the portion is at a position facing the inside of the second processing container.

There is no restriction | limiting in particular as said process in said 2nd processing container, According to the objective, it can select suitably, For example, a mixing process, heat processing, etc. are mentioned suitably.
When a mixing process is performed as the process, the liquid material can be made into a uniform solution or dispersion, and the processing efficiency when the object to be processed is further contacted with the liquid material is excellent. This is advantageous.
The mixing treatment can be suitably performed using a mixing unit. For example, the liquid material in the second processing container is agitated or the liquid material in the second processing container is stirred. It can be performed by circulating using a pump or the like.
When heat treatment is performed as the treatment, it is advantageous in that the object to be treated can be sufficiently dissolved in the liquid material.
In addition, the said heat processing can be performed by installing a heater etc. in said 2nd processing container, for example. The temperature of the heat treatment is not particularly limited and may be appropriately selected depending on the purpose. For example, the object to be treated is a jellyfish, and the liquid material is the jellyfish decomposition composition of the present invention. When it contains, 45 degreeC or more is preferable and 50-70 degreeC is more preferable. In this case, it is advantageous in that the jellyfish can be efficiently decomposed in a short time.
When mixing treatment and heat treatment are performed as the treatment, the liquid material transferred by the first liquid material transfer means can be circulated while being heated inside, and the object to be treated is the liquid material. It is advantageous in that it can be sufficiently dissolved therein to make the liquid into a uniform solution or dispersion.

-Second liquid material transfer means-
The second liquid material transfer means is not particularly limited as long as the liquid material in the second processing container can be transferred into the first processing container, and is appropriately selected according to the purpose. For example, one end is disposed in the second processing container in a state of being immersed in the liquid material in the second processing container, and the other end is connected to the liquid flow inlet in the first processing container. Preferable examples include connected pipes that can pump the liquid material in the second processing container by a pump or the like and transfer it to the first processing container.

There is no restriction | limiting in particular as said pump, For example, it can select suitably from well-known things, such as a plunger pump and a diaphragm pump. Among these, a pump that can be sterilized or sterilized with steam, ozone, or the like in the pump is preferable.
The material, diameter, length, and the like of the pipe are not particularly limited and can be appropriately selected according to the purpose. Examples of the material include resin, metal, and the like. Considering durability against sterilization or sterilization treatment using ozone or ozone, it is preferable to use stainless steel.

The pipe may be provided with a branch pipe. For example, (1) one end is connected to a supply device or generator for ozone or steam for sterilizing or sterilizing the inside of the pipe, and the other end is connected to the pipe. (1) a branch pipe for finally taking out the liquid material, one end of which is connected to the pipe and the other end opened through a cleaning means such as activated carbon. (3) a third branch pipe having one end connected to the pipe and the other end arranged to allow fluid flow in the mixing means in the second processing vessel; (4) one end And a fourth branch pipe in which the other end functions as a drain.
The branch pipe may or may not have a valve, but preferably has at least one valve. The valve may be a manual valve or an electromagnetic valve. When the branch pipe has the valve, the liquid material is transferred (circulated) between the first processing container and the second processing container, sterilization or sterilization processing in the pipe, This is advantageous in that operations such as taking out the liquid material can be performed efficiently.
The method for connecting the branch pipe to the pipe is not particularly limited and can be appropriately selected according to the purpose. For example, any one of welding, connection using a ferrule, a coupler, etc. It may be.

-Other means-
The other means is not particularly limited and may be appropriately selected depending on the purpose. For example, the first processing container, the first liquid material transfer means, the second processing container, A fixing means (for example, a frame, a box, etc.) for integrally fixing the second liquid material transfer means, a moving means (for example, a wheel, etc.) for making the wet processing apparatus movable; Control means (for example, a computer) for controlling the driving of the heating means and the mixing means in the second processing container, the pump in the second liquid material transfer means, and the liquid material after the contact treatment are disposed of outside. In this case, a disposal means for processing to an extent satisfying environmental standards (for example, standards such as COD and BOD) may be used.
In addition, when the wet processing apparatus has the fixing means, the handling property is excellent, and when the wet processing apparatus has the moving means, the wet processing apparatus is placed in a desired place only at a desired timing. It is preferable in that the wet processing apparatus has the control means, and it is preferable in that the processing efficiency is excellent.
There is no restriction | limiting in particular as said discarding means, According to the objective, it can select suitably, For example, an ozone generator etc. are mentioned. The ozone generated by the ozone generator may be designed to be transferred directly into the second processing container and dissolved, or the ozone may be converted using a bubble generator such as an aerator. It may be designed so that it can be transferred into the second processing vessel and dissolved.

-Object to be treated-
In the wet processing apparatus of the present invention, the object to be processed is not particularly limited and can be appropriately selected according to the type and purpose of the contact treatment. For example, the object to be decomposed, the object to be extracted An object, a to-be-plated process target, a to-be-cultured process target, etc. are mentioned.
Examples of the object to be decomposed include those containing a hardly soluble protein, soot, waste water, organic waste liquid, industrial waste, and the like. Examples of the hardly degradable protein include perchloric acid soluble protein (PSP), abnormal prion protein, jellyfish protein and the like. One of these may be used alone, or two or more may be used as objects to be processed. Among these, the wet processing apparatus of the present invention preferably uses the jellyfish protein as the object to be processed, and the wet processing apparatus in this case functions as a “jellyfish decomposing apparatus”.
In addition, when the jellyfish protein is used as the object to be treated, the jellyfish protein having the jellyfish protein is not particularly limited and can be appropriately selected from known ones. For example, water jellyfish, bizen jellyfish, moon jellyfish, Echizen jellyfish, Sakasa jellyfish, Kitayu jellyfish, Andon jellyfish, red jellyfish, and the like. In addition, examples of the component of the jellyfish protein include collagen. The jellyfish includes not only adult jellyfish but also life history in the state of Efila, strobila, planula, polyp, etc.
Examples of the object to be extracted include animals such as plants, insects and fish, and small pieces thereof.
Examples of the object to be plated include metal structures such as a metal substrate.
Suitable examples of the culture target include microorganisms, and specifically include actinomycetes, molds, bacteria, yeasts, and the like.

-Contact treatment-
The contact treatment of the object to be treated with the liquid material is not particularly limited and can be appropriately selected according to the type, purpose, etc. of the object to be treated. For example, decomposition treatment, extraction treatment, plating Treatment, medium contact treatment (culture treatment), and the like. These contact treatments may be used alone or in combination of two or more. Among these, at least one of a decomposition process and an extraction process is preferable.
The conditions such as the time and temperature of the contact treatment are not particularly limited and can be appropriately selected according to the purpose. Note that the time for the contact treatment between the object to be treated and the liquid material in the first processing container appropriately adjusts the flow rate of the liquid material flowing from the liquid flow inlet in the first processing container. Thus, specifically, it can be carried out by appropriately adjusting the transfer speed of the liquid material by the second liquid material transfer means.

-Liquid material-
There is no restriction | limiting in particular as said liquid substance, According to the objective, it can select suitably, For example, what contains the degradation enzyme of the said to-be-processed object, an extraction solvent, a plating agent, a culture medium etc. is mentioned. The liquid material may be oily or aqueous, but is preferably aqueous and is preferably an aqueous solution from the viewpoint of disposal.

  When the object to be treated is the hardly soluble protein, the liquid material preferably contains at least one of a degrading enzyme of the hardly soluble protein and a microorganism that produces the degrading enzyme. When the object to be treated is the jellyfish protein, the liquid material preferably contains at least one of the jellyfish protein degrading enzyme and the microorganism producing the degrading enzyme. In the former case, the wet processing apparatus functions as a “hardly soluble protein decomposing apparatus”, and in the latter case, the wet processing apparatus functions as a “jellyfish decomposing apparatus”.

The hardly soluble protein degrading enzyme and the jellyfish protein degrading enzyme are not particularly limited as long as they are capable of degrading the hardly soluble protein and the jellyfish protein, respectively. Although it can select, the protease which actinomycetes produces is mentioned suitably.
In addition, the microorganism that produces the degrading enzyme of at least one of the hardly soluble protein and the jellyfish protein is not particularly limited and may be appropriately selected depending on the intended purpose. It is done.

As the actinomycetes, for example, those selected from Streptomyces actinomycetes are preferable.
As the Streptomyces genus actinomycetes, for example, those selected from sp. In addition, the said Streptomyces sp (Streptomyces sp.) Exists abundantly in soil, and pathogenicity is not confirmed.
As the SP species actinomycetes, for example, those having the following A to C, preferably A to D bacteriological properties are suitably exemplified, such as 99-GP-2D-5 strain (FERM P-19336) and More preferred are those selected from the derived strains.
A. Morphological properties (1) A relatively long wavy aerial hyphae is elongated from a branched basic hyphae and rarely has a hook or loop shape.
(2) The mature spore chain links 10 to 50 oval spore, and the size of the spore is about 0.6 to 0.7 × 0.8 to 1.0 micron.
(3) The spore surface is smooth.
(4) No roticular branch, mycelial bundle, spore and motility spore are observed.
B. The cell wall composition contains LL-type 2,6-diaminopimelic acid.
C. 90% or more, preferably 95% or more homology to Streptomyces actinomycetes due to a partial base sequence (400 to 500 bp) of 16S ribosomal RNA gene.
D. Growth state in various media (1) On the growth of yeast / malt agar medium (ISP-medium 2, cultured at 27 ° C.) and light yellow [2ea, LtWheat], grayish white [1 dc, Putty] to bright olive ash [ 1 1/2 ge, Lt Olive Gray] slightly aerial mycelia were formed, and no soluble pigment was observed.
(2) Oatmeal agar medium (ISP-medium 3, 27 ° C. culture), colorless to light yellow [1 1/2 ca, Cream], white aerial mycelia grow slightly, soluble pigment is observed I can't.
(3) On a starch / inorganic salt agar medium (ISP-medium 4, 27 ° C. culture) and colorless growth, white aerial mycelia are slightly formed and no soluble pigment is observed.
(4) Glycerin / asparagine agar medium (ISP-medium 5, 27 ° C. culture), growth is colorless, aerial hyphae do not grow, and no soluble pigment is observed.
(5) On a sucrose / nitrate agar medium (27 ° C. culture), colorless growth, white aerial mycelia are slightly formed and no soluble pigment is observed.

  Examples of the derived strain of Streptomyces sp. 99-GP-2D-5 (FERM P-19336) include progeny of Streptomyces sp. 99-GP-2D-5 and Streptomyces sp. 99-GP-2D. -5 strains which are artificial or natural mutants and have a protease-producing ability capable of degrading a hardly degradable protein. The Streptomyces sp. 99-GP-2D-5 strain, like other Streptomyces strains, is susceptible to changes in its properties, for example, by artificial mutation means using ultraviolet rays, X-rays, drugs, and the like. Can be mutated.

The “jellyfish degrading composition” of the present invention is not particularly limited as long as it contains the “jellyfish protein degrading enzyme”, and the above-mentioned Streptomyces sp. 99-GP-2D-5 strain (FERM P-19336), etc. The culture solution of actinomycetes itself may be used, or the culture solution may be treated (crudely purified product), or the culture solution may be purified (purified product). Furthermore, if necessary, other components may be added or mixed as appropriate.
The content of the jellyfish degrading enzyme in the “jellyfish decomposing composition” of the present invention may be 100% by mass or less, and if it is less than 100% by mass, the enzyme Examples of the components other than those include a culture solution component, water, seawater, and the like.

The culture of the microorganism producing at least one of the degrading protein degrading enzyme and the jellyfish protein degrading enzyme is not particularly limited and may be appropriately selected depending on the intended purpose. For example, a known actinomycete A culture medium can be used suitably, FG culture medium mentioned later can be used especially suitably, and it can carry out suitably on aerobic conditions.
The culture solution obtained as a result of culturing the microorganism contains the degrading enzyme (degrading enzyme of the hardly soluble protein or degrading enzyme of jellyfish protein). It can be suitably used as a “composition”.

  The method for purifying the “persistent protein degrading enzyme” to the “jellyfish protein degrading enzyme” from the culture solution is not particularly limited and may be appropriately selected depending on the intended purpose. For example, ammonium sulfate or ethanol precipitation , Acid extraction, dialysis, anion or cation exchange chromatography, phosphocellulose chromatography, hydrophobic interaction chromatography, affinity chromatography, hydroxyapatite chromatography, lectin chromatography, high performance liquid chromatography, etc. It is done. In the case of the affinity chromatography, the column used there may be, for example, a column to which an antibody such as a monoclonal antibody against “degrading enzyme of jellyfish protein” to “degrading enzyme of refractory protein” is bound, or “jellyfish protein Columns in which a normal peptide tag is added to “degrading enzyme of” or “degrading enzyme of hardly degradable protein” and a substance having affinity for this peptide tag is bound.

  Examples of the carbon source of the medium include glucose, potato starch, dextrin and the like. These may be used individually by 1 type and may use 2 or more types together. Examples of the inorganic nitrogen source and the organic nitrogen source in the medium include yeast extract, tryptose, corn stee liquor, soy flour, meat extract, tomato puree and the like. These may be used individually by 1 type and may use 2 or more types together. The medium may be supplemented with inorganic salts such as sodium chloride, calcium carbonate, magnesium sulfate, copper sulfate, iron sulfate, zinc sulfate, manganese chloride, cobalt chloride, and phosphate as required, and amino acids. Organic substances such as vitamins and nucleic acids may be added.

  The culture method is not particularly limited and may be appropriately selected depending on the intended purpose. For example, the liquid culture method is preferable, and the shaking culture method is more preferable. The culture temperature is preferably about 20 ° C. to 40 ° C., and the culture pH is preferably weakly acidic to alkaline. In the case of the liquid culture, when the culture is normally performed for 4 to 6 days, the above-mentioned degrading enzyme (protease) is produced and accumulated in the culture solution, but the cultivation is stopped when the production and accumulation amount reaches the maximum. In the present invention, the culture solution (protease-containing product) obtained by separating the bacterial cells and the culture solution is used in the present invention as a “jellyfish-decomposing composition”, “degrading enzyme of jellyfish protein”, “refractory protein” It can be suitably used as a “degrading enzyme”.

The optimum pH of the degrading enzyme of the hardly degradable protein and the degrading enzyme of the jellyfish protein is about 9 to 12, and the optimum temperature is about 60 to 70 ° C.
Protease is preferable among the hardly degradable protein degrading enzyme and the jellyfish protein degrading enzyme, and more preferably a protease containing YDLVGGGDAYYIG as an N-terminal amino acid sequence.

  If the above-mentioned wet processing apparatus is used to decompose the hardly decomposable protein, the method of degrading the hardly decomposable protein of the present invention, which will be described later, has been carried out, and the jellyfish (jellyfish protein) is decomposed. If it carries out, it will have implemented the decomposition | disassembly method of the following jellyfish of this invention.

(Jellyfish decomposition method)
The method for decomposing jellyfish of the present invention includes at least the contact treatment of the above-mentioned “composition for decomposing jellyfish” of the present invention with respect to the jellyfish as described above, and further, other treatments appropriately selected as necessary. including.
Examples of the other processing include waste liquid processing.
The method for decomposing the jellyfish can be appropriately carried out using a known apparatus. For example, the jellyfish is contained in a container, the jellyfish decomposing composition is added to the container, and the jellyfish is decomposed. Can be carried out by contact-treating the jellyfish-decomposing composition with the jellyfish-decomposing composition, but it can be particularly suitably carried out by using the above-described wet processing apparatus of the present invention.

(Method of degrading persistent proteins)
The method for degrading a hardly decomposable protein according to the present invention is the above-described hardly degradable protein degrading enzyme from the lower part of the first treating container with respect to the above hardly resistant protein contained in the first treating container. A liquid treatment containing the above-mentioned contact treatment step in which the above-mentioned contact treatment is performed, and the liquid material reaching a predetermined amount in the first treatment container is transferred to the second treatment container, and the second treatment container A heating and circulating step of circulating while heating in the processing vessel; and a liquid material circulating step of transferring and circulating the liquid in the second processing vessel into the first processing vessel described above, and further necessary Other processes appropriately selected according to the above are included.
The other treatment is not particularly limited and may be appropriately selected depending on the intended purpose. For example, the liquid material removal treatment, waste liquid treatment, sterilization or sterilization treatment of the first treatment container, and washing Processing.
In addition, it is preferable that the liquid material contains the above-described degradable protein degrading enzyme, and the method for degrading the hardly degradable protein is preferably performed using the above-described wet processing apparatus of the present invention. Can do.

  Examples of the present invention will be described below, but the present invention is not limited to these examples. This example is an example in which the “wet treatment apparatus” of the present invention is used as a jellyfish decomposing apparatus, and the “jellyfish decomposing composition” of the present invention is used as the liquid substance. The jellyfish decomposition performed using the apparatus corresponds to the “degradable protein decomposition method” and the “jellyfish decomposition method” of the present invention.

  FIG. 1 is a schematic explanatory view showing an embodiment of the wet processing apparatus of the present invention. The wet processing apparatus shown in FIG. 1 includes a decomposition tank 1 as the first processing container and the first liquid. It has a siphon tube 3 as a material transfer means, a circulation / warming tank 5 as the second processing container, and a pipe as the second liquid material transfer means.

Here, the decomposition tank 1 is a cylindrical tank, is made of a transparent resin, and is disposed in an upright state. The decomposition tank 1 in a standing state has an upper end opened. In the lower part near the bottom surface of the decomposition tank 1, there are provided a liquid distribution inlet through which the liquid material flows into the interior and a liquid distribution outlet through which the liquid material flows out. In addition, a flow meter (not shown) is provided in the vicinity of the liquid distribution inlet so that the flow rate of the liquid material flowing into the decomposition tank 1 can be measured.
Inside the decomposition tank 1, a partition tank 2 as the casing having a mesh smaller than the diameter of the liquid flow inlet and the liquid flow outlet is disposed.

  Here, the siphon tube 3 is a tubular structure (pipe) having openings at both ends, and is made of a transparent resin. One end of the siphon tube 3 is connected to the liquid flow outlet in the decomposition tank 1 so that the liquid material cannot flow out, and is bent substantially vertically and upward in the vicinity of the one end. Further, the siphon tube 3 is disposed in the decomposition tank 1 at an arbitrary height position lower than the maximum height of the liquid substance accommodated therein, and the other end thereof is in a direction in which gravity is applied and is connected to the circulation / heating tank 5. It is bent toward the opposite direction. The siphon tube 3 can transfer the liquid material in the decomposition tank 1 to the outside from the liquid distribution outlet according to the principle of siphon when the liquid material flowing into the decomposition tank 1 reaches a predetermined amount.

  Here, the circulation / heating tank 5 is a rectangular parallelepiped water tank and is made of a transparent resin. The circulation / warming tank 5 is positioned below the decomposition tank 1, and the opening end of the siphon tube 3 is disposed at a position facing the inside of the circulation / heating tank 5. The circulation / heating tank 5 includes a heating tube 8. A heater 9 is disposed at the center in the vertical direction of the heating tube 8.

  The pipe as the second liquid material transfer means is a pipe for transferring the liquid material in the circulation / warming tank 5 into the decomposition tank 1, and is made of stainless steel here, and one end is circulated / heated. In the state immersed in the liquid in the tank 5, it is arranged in the circulation / heating tank 5, and the other end is connected to the liquid flow inlet in the decomposition tank 1. In the middle of the pipe, a liquid feed pump 4 as the pump is connected, and when the liquid feed pump 4 is driven, the liquid material in the circulation / heating tank 5 is pumped up, and the inside of the decomposition tank 1 It is transferred to.

A first branch pipe, a second branch pipe, a third branch pipe, and a fourth branch pipe are connected to the pipe. The connection of these branch pipes is screwed.
Here, the first branch pipe is made of stainless steel, one end is connected to an ozone supply device that supplies ozone for sterilizing or sterilizing the inside of the pipe, the other end is connected to the pipe, and the valve And a backflow prevention valve 12 capable of preventing the backflow of ozone.
Here, the second branch pipe is made of stainless steel and is a branch pipe for finally taking out the liquid material, one end of which is connected to the pipe and the other end via the activated carbon treatment cylinder 15. It is open and has a gate valve 13.
Here, the third branch pipe is made of stainless steel, one end is connected to the pipe, and the other end is arranged to allow fluid to flow in the heating pipe 8 in the circulation / warming tank 5. A valve 7 is provided.
Here, the fourth branch pipe is made of stainless steel, one end is connected to the pipe, the other end functions as a drain, and includes a gate valve 6.

  In the wet processing apparatus shown in FIG. 1, each apparatus element is integrally fixed by a frame or the like (not shown), and a movable wheel is provided at the lower part, which is designed to be portable.

  In the wet processing apparatus shown in FIG. 1, contact processing of the object to be processed is performed. For example, when the wet processing apparatus is a jellyfish decomposition apparatus, in this case, the object to be processed is described. Becomes a “jellyfish (jellyfish protein)”, and the wet processing apparatus becomes a “jellyfish decomposition apparatus”.

  The jellyfish landed from the sea is first put into a partition tank 2 arranged in the decomposition tank 1. At this time, the jellyfish may be charged as it is, or may be charged after being crushed in advance and fragmented from the viewpoint of improving the decomposition processing speed.

The liquid used here is water containing the jellyfish decomposing composition (concentration: 0.003 mass%) and flows into the jellyfish contained in the partition tank 2 from the liquid flow inlet. To be contacted.
As the jellyfish decomposition composition, here, a strain deposited under the name of “Streptomyces sp. 99-GP-2D-5” at the Patent Organism Depositary of the National Institute of Advanced Industrial Science and Technology (Accession number) A culture medium containing an enzyme (jellyfish proteolytic enzyme) produced by FERM P-19336).

  Specifically, the culture solution containing the enzyme (jellyfish proteolytic enzyme) was prepared as follows. That is, the Streptomyces sp. 99-GP-2D-5 strain was cultured using an FG medium having the following composition. After culturing, the cells are removed by centrifugation to obtain a culture supernatant, and then the culture supernatant is saturated with 80% ammonium sulfate, and the ammonium sulfate saturated precipitate obtained by centrifugation is added with 0.05M phosphate buffer (pH 6. The crudely purified product of the enzyme obtained by dialysis against 8) was used as the “composition for jellyfish decomposition”.

<Composition of FG medium (pH 7.0)>
Fish extract (manufactured by Kyokuto Pharmaceutical Co., Ltd.) ... 1.0g
Yeast extract (Difco) ... 1.0g
Glucose (made by Wako Pure Chemical Industries) ... 2.0g
Magnesium sulfate heptahydrate (Wako Pure Chemical Industries) 0.05g
Calcium carbonate (manufactured by Wako Pure Chemical Industries, Ltd.) ... 0.32g
Deionized water ... 100ml

Here, the physicochemical properties of the enzyme (jellyfish proteolytic enzyme) contained in the jellyfish decomposing composition were analyzed as follows. That is, when the molecular weight of the enzyme (jellyfish proteolytic enzyme) was measured by mass spectrometry using a mass spectrum analyzer (manufactured by JEOL Ltd.), it was 19,327.
Further, when the N-terminal amino acid sequence was examined by the Edman degradation method using HP G1005A Protein Sequencing System (manufactured by Hewlett-Packard), it was found that YDLVGGDAYYIG was contained.

  In the “jellyfish decomposing apparatus” which is an embodiment of the wet processing apparatus shown in FIG. 1, the jellyfish decomposing composition previously stored in the circulation / heating tank 5 is connected to the gate valve 14 in the pipe “ The gate valve 6, the gate valve 7, the gate valve 11, and the gate valve 13 are set to “closed”, the liquid feed pump 4 provided in the pipe is driven to pump up, and transferred into the decomposition tank 1. . Then, the jellyfish decomposing composition flows into the decomposing tank 1 from the liquid flow inlet, contacts the jellyfish accommodated in the partition tank 2, and the jellyfish is contact-treated by the jellyfish decomposing composition. . At this time, the jellyfish is contact-treated so as to be pushed upward by the jellyfish decomposing composition flowing into the decomposition tank 1 from the bottom. That is, the jellyfish is about to fall in the direction of gravity, whereas the jellyfish decomposing composition comes into contact with the jellyfish about to fall. Therefore, due to the synergistic effect of gravity added to the jellyfish and the fluid pressure of the jellyfish decomposing composition, the contact treatment is effectively performed, and the jellyfish (the jellyfish protein which is the hardly degradable protein) is obtained. ) Is efficiently decomposed.

  Part of the jellyfish decomposition composition flowing into the decomposition tank 1 flows out into the siphon pipe 3 from the liquid flow outlet provided at the lower part of the decomposition tank 1. Then, along with an increase in the amount of liquid of the jellyfish decomposition composition flowing into the decomposition tank 1 (increase in the water level), it is connected to the liquid distribution outlet in the decomposition tank 1 and extends upward from the vicinity of the liquid distribution outlet. In the siphon tube 3 that is provided and curves downward near the upper part of the decomposition tank 1, the liquid amount of the jellyfish decomposition composition increases (the water level increases) at the same rate. When the liquid amount (water level) of the jellyfish decomposition composition in the siphon tube 3 reaches the curved portion of the siphon tube 3, the jellyfish decomposition composition in the decomposition tank 1 is converted into the liquid state according to the principle of siphon. The material is continuously transferred from the distribution outlet to the outside through the siphon tube 3, and continuously transferred from the tip 17 of the siphon tube 3 into the circulation / heating tank 5. At this time, in the partition tank 2, the jellyfish falls by its own weight as the jellyfish decomposition composition is reduced, and when all the jellyfish decomposition composition is transferred to the outside, the partition tank 2 It collides with the bottom surface of the plate and breaks into small pieces due to the impact and the like.

  The composition for decomposing jellyfish transferred to the circulation / warming tank 5 is pumped up again by driving the liquid feed pump 4 provided on the pipe, and transferred to the decomposition tank 1 again. Thereby, the second contact process is performed in the decomposition tank 1. At this time, the gate valve 14 in the piping is “open”, and the gate valve 6, the gate valve 7, the gate valve 11 and the gate valve 13 are “closed”. In addition, if the liquid feed pump 4 provided in the said piping is continuously driven, the re-transfer process in the decomposition tank 1 and the recontact process in the decomposition tank 1 can be repeated continuously. By performing this contact treatment a plurality of times, the jellyfish accommodated in the partition tank 2 are completely decomposed and dissolved in the jellyfish decomposing composition.

  Further, in the circulation / heating tank 5, if necessary, the jellyfish decomposition composition transferred by the siphon tube 3 is allowed to pass through the heating tube 8 and circulate to be heated or reacted. it can. That is, the jellyfish decomposing composition is uniformly mixed by passing through the heating tube 8 and circulating in the circulation / heating tank 5, and is also mixed into the jellyfish decomposing composition by the heater 9. By heating to the optimum temperature of the enzyme contained (if the temperature of the jellyfish decomposition composition has already reached the optimum temperature of the enzyme contained therein, it is necessary to operate the heater 9 The jellyfish degrading solution as a solution in which the jellyfish protein is uniformly dissolved as a result of complete and efficient degradation of undegraded jellyfish fragments (jellyfish protein) contained in the jellyfish decomposing composition A composition is prepared. In order to allow the jellyfish decomposition composition in the circulation / heating tank 5 to pass through the heating pipe 8 and circulate in the circulation / heating tank 5, the gate valve 7 in the pipe is "open". The gate valve 6, the gate valve 13, and the gate valve 14 are set to “closed”, and the liquid feed pump 4 provided in the pipe is driven. Further, at this time, all of the jellyfish decomposition composition in the circulation / heating tank 5 may be circulated in the circulation / heating tank 5 through the heating pipe 8, or the circulation / heating tank. Even if a part of the composition for decomposing jellyfish in the warm bath 5 is passed through the heating tube 8 and circulated in the circulation / heating bath 5, the remaining portion may be transferred into the decomposition bath 1. Good.

  After the jellyfish decomposition process in the decomposition tank 1 is completed, the jellyfish decomposition composition (containing the decomposed jellyfish protein) which is a decomposition treatment liquid may be disposed of or temporarily collected. Thereafter, useful proteins such as collagen, lipids and the like may be further extracted and discarded.

In the case of disposal, it is necessary to purify until reaching the water quality standard that can be discharged to the outside. In this case, the gate valve 11 is “opened”, the gate valve 6, the gate valve 7, the gate valve 13 and the gate valve 14 are “closed”, and the ozone generated from the ozone generator 10 is allowed to pass through the backflow prevention valve 12. The jellyfish decomposition composition (containing the decomposed jellyfish protein) after the decomposition treatment, which is introduced into the circulation / heating tank 5 and accommodated in the circulation / heating tank 5, is treated with ozone, By oxidative decomposition, the water quality can be purified to a level that can be discharged to the outside.
After the ozone treatment, the organic matter in the composition for decomposing jellyfish after decomposition (containing the decomposed jellyfish protein) has reached the water quality standard that can be discharged, and then the gate valve 13 is opened. By leaving the gate valve 6 and the gate valve 14 "closed" and driving the liquid feed pump 4 to pass through the activated carbon treatment cylinder 15, it can be discharged to the outside as a treatment liquid 18 that can be discharged into the environment. .

  In the case of recovering useful proteins such as collagen and useful substances such as lipids from the jellyfish decomposing composition (containing decomposed jellyfish protein), the ozone treatment is not performed, and the gate valve 6 is not used. The decomposition treatment liquid 16 is obtained by setting the gate valve 7, the gate valve 13, and the gate valve 14 to “closed” and driving the liquid feed pump 4.

  The jellyfish was decomposed by the above-described jellyfish decomposition apparatus using various treatment liquids shown in Table 1, and the change over time in weight loss (%) of the jellyfish was measured. The results are shown in Table 1. The weight loss of the moon jellyfish is a value obtained by dividing the weight of the jellyfish before treatment (wet weight) by the weight of the residue after treatment (wet weight) divided by the weight of jellyfish before treatment (wet weight) and multiplying by 100. The jellyfish weight here is a value measured without drying the isolated jellyfish individual. The numbers in Table 1 indicate (average value ± standard deviation).

  As is clear from the results in Table 1, in each case where the jellyfish decomposition composition of the present invention was not used, the jellyfish decomposition was not complete even after 24 hours. When this was used, especially when it was heated to 50 ° C., the moon jellyfish could be completely and easily decomposed in about 1 hour.

The above-described wet processing apparatus (jellyfish decomposition apparatus) according to the embodiment of the present invention has the following features. That is, (1) No equipment such as a stirring device or a temperature control device is required in the decomposition tank 1. (2) Since no stirring device is required in the decomposition tank 1, the size of the device can be easily increased. (3) When the liquid material (processing liquid) in the decomposition tank 1 reaches the same height as the upper curved portion of the siphon tube 3, the liquid material (processing liquid) is continuously circulated by the principle of siphon. Since it is transferred to the heating tank 5, special equipment such as a pump for transfer is unnecessary, and problems such as overflow of the liquid material in the decomposition tank 1 do not occur. (4) Since a stirrer is unnecessary, it is safe and can be operated with one pump, so that maintenance management is extremely easy, and operation is possible at low cost. (5) A jellyfish (jellyfish protein) or the like can be continuously decomposed. (6) Since the circulation / heating tank 5 can be used as a waste liquid treatment tank, the number of tanks is small, it can be designed at low cost, and maintenance is easy. (7) Although the jellyfish is semi-transparent and difficult to see even in a transparent liquid, the jellyfish solid is exposed at the bottom of the decomposition tank 1 when the liquid is transferred from the siphon tube 3 to the outside. It is easy to determine the degree of jellyfish decomposition. (8) When the liquid material is transferred from the siphon tube 3 to the outside, the jellyfish solids fall to the bottom of the decomposition tank 1 and are stacked together. As a result, the lower jellyfish are crushed. When the jellyfish are physically crushed and the number of fine pieces increases, there is an advantage that the surface area of the jellyfish to be decomposed increases and the processing becomes efficient.
Furthermore, according to the method for degrading a hardly degradable protein and the method for degrading jellyfish according to the present invention, the jellyfish protein, which is the hardly degradable protein, can be completely decomposed efficiently in a short time at a low cost.

The wet processing apparatus of the present invention can be suitably used in various fields. For example, the persistent protein decomposing apparatus, the jellyfish (jellyfish protein) decomposing apparatus, the extracting apparatus, the plating apparatus, and the microorganism culture apparatus. , Etc. can be suitably used.
The composition for decomposing jellyfish of the present invention can be suitably used for the decomposition process of the jellyfish.
The method for degrading a hardly degradable protein of the present invention can be suitably used for degrading the aforementioned hardly degradable protein such as jellyfish protein.
The method for decomposing jellyfish protein of the present invention can be suitably used for decomposing various jellyfish.

DESCRIPTION OF SYMBOLS 1 ... Decomposition tank 2 ... Partition tank 3 ... Siphon tube 4 ... Liquid feed pump 5 ... Circulation / warming tank 6 ... Gate valve 7 ... -Gate valve 8 ... Heating tube 9 ... Heater 10 ... Ozone generator 11 ... Gate valve 12 ... Check valve 13 ... Gate valve 14 ... Gate valve 15 ... Activated carbon treatment tube 16 ... Jellyfish decomposition treatment water 17 ... Siphon tube passage treatment liquid outlet 18 ... Activated carbon treatment tube passage clean waste water

Claims (4)

A composition for decomposing jellyfish, comprising a protease produced by actinomycetes. The composition for jellyfish decomposition according to claim 1, wherein the actinomycete is selected from Streptomyces actinomycetes. The composition for jellyfish degradation according to claim 2, wherein the Streptomyces genus actinomycetes is selected from sp. The jellyfish decomposing composition according to claim 3, wherein the SP species actinomycetes are selected from 99-GP-2D-5 strain (FERM P-19336) and its derived strain.