JP2009102256A - Method for producing extract material of cultured mycelium and device for culturing mycelium - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide technologies for the production of the extract material of cultured mycelium, obtaining the extract material of cultured mycelium in a shorter period of time, and also applicable for various plant fibrous raw materials. <P>SOLUTION: This extract of cultured material is obtained by inoculating the mycelium of a Basidiomycete on a solid medium containing a plant fibrous raw material, holding it under an environment for growing the mycelium after agitating or while agitating the solid medium inoculated with the mycelium by imparting the repeated movement of falling down and elevation and obtaining the extract of the cultured material from the resultant cultured material. The production of the extract of the cultured mycelium can be performed suitably by a device for culturing the mycelium, equipped with a culturing container having a putting-in port of the solid culturing medium and the mycelium of the Basidiomycete, a lid for hermetically closing the putting-in port and a ventilation hole for passing through air, and a rotating means for rotating the culturing container by a horizontal or an inclined rotary shaft. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to a method for producing a mycelium culture extract and a mycelium culture apparatus, and more specifically, a method for culturing mycelia of basidiomycetes such as shiitake mushrooms and mannen mushrooms using a solid medium and obtaining the culture extract. And a culture apparatus.

  The mycelium of basidiomycetes can be roughly classified into liquid culture methods and solid culture methods. The liquid culture methods include short culture periods, easy maintenance of pure culture conditions, etc. There are advantages. In contrast, the solid culture method inoculates mycelium on a solid medium containing plant fiber raw materials such as bagasse, wheat bran, rice bran, etc., and stops the culture before the formation of fruit bodies to obtain the mycelium. However, this solid culture method can cultivate mycelium that has grown closer to natural conditions, and the solid medium used also has plant fiber. There are unique advantages in that components derived from raw materials are included and can be extracted together.

  Production of a basidiomycete mycelium culture extract using a solid medium generally comprises the following steps. That is, (i) culture of the original mycelium (so-called inoculum), (ii) inoculation of the inoculum into the solid medium, (iii) culture on the solid medium, and aging of the medium, (iv) the aged medium It is the process of extracting from.

  Of these, culturing on a solid medium and ripening of the medium usually require a long period of about 4 months and occupy the majority of the whole process. This is because mycelium growth on a solid medium is: (i) mycelial elongation on the surface of the solid medium, (ii) mycelial infiltration into the medium, (iii) spreading throughout the medium, (iv) mycelia This is because it is necessary to go through many stages such as body film formation and maturation (the state immediately before the occurrence of fruit bodies and mushrooms), and each stage progresses slowly. In addition, for the aging of the medium, it is necessary to take steps of (v) decomposition and consumption of the medium components by the mycelium at the elongation and infiltration destination, and (vi) moderate equilibration of these. Through these steps, the pH of the medium decreases and at the same time the water-soluble components increase, and the aging of the culture medium is achieved. This has led to an increase in the manufacturing period, which has been one of the causes of increased costs. In addition, since these ripenings are sequentially performed from a limited site where the mycelium is inoculated, a difference in the ripening level depending on the site tends to occur in the whole culture medium, which causes problems in production and quality control. Furthermore, prolonged culture days have been a very disadvantageous factor in the industry, such as increasing the risk of contamination during that time.

As a method for shortening the culture period on a solid medium, Patent Document 1 described below describes a method of culturing shiitake bacteria using a culture bag made of a plastic film of a specific material and ripening in a shorter period of time than before. Has been. Patent Document 2 below describes a method of cultivating and harvesting shiitake mushrooms in a shorter period of time than conventional methods by culturing inoculum by sequentially performing liquid culture and solid culture. Further, in Patent Document 3 below, in the solid culture of Ganoderma mycelium, Ganoderma mycelium is added to the whole medium by using a solid medium made of rice husk instead of the whole medium based on bagasse. It is described that it can be propagated in a short period of 30 to 45 days for breeding.
Japanese Patent Laid-Open No. 08-172906 JP 08-228593 A Japanese Patent Application Laid-Open No. 07-79738

  However, the methods of Patent Document 1 and Patent Document 2 are aimed at obtaining fruit bodies of basidiomycetes in any case, and are not techniques that contribute to shortening the culture period of mycelium. Moreover, according to the method of the above-mentioned patent document 3, since it is limited to the solid medium using rice husk as a raw material, the mycelium culture extraction including the component derived from the plant fiber raw material of the solid medium used together with the mycelium component In obtaining a product, there was a problem that it could not be applied to other plant fiber raw materials such as bagasse.

  Accordingly, an object of the present invention is a technique for producing a mycelium culture extract, a technique capable of obtaining a mycelium culture extract in a shorter period of time, and applicable to various plant fiber raw materials. Is to provide.

  The present inventors have found that the subsequent culture period can be remarkably shortened by a simple treatment in which a mycelium of basidiomycetes is inoculated into a solid medium and then stirred in a culture container such as a tank. It came to complete.

  That is, in the method for producing a mycelium culture extract of the present invention, a mycelium of basidiomycetes is inoculated into a solid medium containing a plant fiber raw material, and the solid medium inoculated with the mycelium is subjected to repeated movements of falling and rising. After stirring by applying to the solid medium, or while stirring, it is maintained in a culture environment where the mycelium grows, and a culture extract is obtained from the obtained culture.

  According to the method for producing the mycelium culture extract of the present invention, the inoculated site of the basidiomycete can be spread throughout the solid medium. ) Elongation of mycelium on the surface of the solid medium can be performed from multiple directions instead of one direction, (ii) Infiltration of mycelia into the medium can be performed simultaneously, and (iii) The spread of hyphae can be achieved quickly. As a result, the culture period can be shortened to about half that of the prior art by efficiently reducing the pH of the medium and increasing the water-soluble component (solid content in the extract). Furthermore, since the aging of the medium proceeds uniformly throughout, it is very convenient in terms of production management and quality control in terms of grasping the culture state or ease of predicting the quality of the final product.

  In the method for producing a mycelium culture extract of the present invention, a solid medium inoculated with the mycelium is placed in a culture container, and the culture container is rotated to impart a repeated movement of falling and rising to the solid medium. It is preferable. According to this, the repeated motion of dropping and rising can be efficiently imparted to the solid medium, and basidiomycete attachment sites can be spread throughout the solid medium.

  In the method for producing a mycelium culture extract of the present invention, it is preferable that the plant fiber raw material is prepared from a crustacean plant.

  Further, the plant fiber raw material is prepared from one or more selected from bagasse, corn stover, wheat bran, rice bran, rice straw, rice husk, straw, bear cocoon, and bamboo. preferable.

  Furthermore, it is preferable that the basidiomycete is selected from mannen mushrooms, bukuro, kofukisarokoshikake, kawara mushrooms, shiitake mushrooms, hinoki mushrooms, mai mushrooms, enoki mushrooms, shimeji mushrooms, yamabushi mushrooms, and agaricus.

  On the other hand, the mycelium culturing apparatus of the present invention is a culture container having an inlet for introducing a solid medium and mycelia of basidiomycetes, a lid for sealing the inlet, and a vent for circulating air. And a rotating means for rotating the culture vessel by a horizontal or inclined rotating shaft.

  According to the mycelium culturing apparatus of the present invention, since the mycelium of the solid medium and the basidiomycetous fungus is introduced and cultured, and the rotating means for rotating the same, the mycelium culture extract It can be used suitably for a manufacturing method, and its scale-up is also easy.

  In the mycelium culturing apparatus of the present invention, the rotating means is provided on the support table so as to support the culture container so that the culture container can be removed and rotated. It is preferable that a plurality of rotating rollers to be supported and a drive device that rotates at least one rotating roller that is in rolling contact with the side surface of the culture vessel.

  According to this, since the plurality of rotating rollers support the culture container in which the solid medium and basidiomycetous mycelium are charged, the culture container can be easily removed from the rotating means, and is sterilized, cultured, and extracted. Convenient to move to the place provided for.

  In the mycelium culture device of the present invention, the culture vessel is preferably a substantially cylindrical culture vessel. According to this, it is suitable for transmission of the rotational force via the rotating roller.

  Moreover, it is preferable that a desorption nozzle is provided on the outside air side of the vent hole of the culture vessel, and a tubular vent tube is further attached to the desorption nozzle. According to this, aeration for the growth of basidiomycete can be appropriately performed.

  Furthermore, the culture vessel is preferably a culture vessel that can be autoclaved. According to this, since the solid medium can be sterilized after being put into the culture container, contamination of germs can be prevented, and it is not necessary to perform the operation of putting into the culture container aseptically. Also convenient.

  According to the method for producing a mycelium culture extract of the present invention, a basidiomycete mycelium culture extract can be obtained in a shorter period of time as compared with the conventional production method. In addition, since the aging of the medium proceeds uniformly throughout, it becomes easy to grasp the culture state and predict the quality of the final product.

  On the other hand, the mycelium culturing apparatus of the present invention can be suitably used in the method for producing the mycelium culture extract of the present invention, and its scale-up is easy. In addition, when a tank-like and autoclavable container is used as the culture container, it is possible to fill the medium and inoculate the inoculum with good workability, so the risk of contamination is expected to be reduced. it can.

  The method for producing a mycelium culture extract of the present invention comprises inoculating a mycelium of basidiomycetes on a solid medium containing a plant fiber raw material, and subjecting the solid medium inoculated with the mycelium to repeated movements of falling and rising. After stirring by applying to the medium, or while stirring, the culture is maintained in a culture environment where the mycelium grows, and a culture extract is obtained from the obtained culture.

  There are no particular restrictions on the basidiomycetes used in the present invention, for example, medicinal mushrooms such as mannen mushrooms, bukuryoku, kofukisarokoshikake, kawara mushrooms, shiitake mushrooms, hinoki mushrooms, mai mushrooms, enoki mushrooms, shimeji mushrooms, yamabushi mushrooms, agaricus, etc. There are various kinds of edible rice cakes. These basidiomycetes can be cultured in advance by an appropriate method such as liquid culture or solid culture, and used as mycelium (inoculum for inoculation).

  As the plant fiber raw material used in the present invention, one prepared from a plant containing lignin is preferably used. As the lignin-containing plant, scallops such as bagasse (squeezed sugar cane), corn stover, wheat bran, rice bran, rice straw, and straw are preferably used. In addition, bear cucumber, bamboo, rice husk etc. can be used. Particularly preferably, a culture medium containing at least one selected from bagasse, bear stems and corn stalks and rice bran is used. Moreover, you may add and mix yeast extract, dry yeast, chlorella, spirulina, corn meal, okara, etc. as another nutrient component as needed.

  As a solid culture medium used for this invention, what mix | blended the said plant fiber raw materials 80-90% and other nutrient components 20-10%, and prepared so that a water | moisture content may be 60-80% should be used. preferable. Moreover, in order to prevent contamination of germs in the solid medium, it is preferable that the paste has been subjected to high-pressure steam sterilization according to a conventional method. Sterilization may be performed by autoclaving the whole culture container after filling the culture medium with the solid medium, or sterilizing the sterilized solid medium in a clean room or the like in a culture container that has been previously sterilized. May be filled.

  In the method for producing a mycelium culture extract of the present invention, the mycelium of the basidiomycete is inoculated into a solid medium containing the plant fiber raw material. Specifically, for example, when culturing in a tank-like culture container, the mycelium is added to the tank by a method suitable for maintaining sterility after filling and sterilization of the solid medium are completed. At that time, if a mycelium cultured in liquid is used as an inoculum, it should be added aseptically into the tank using a vent provided in the tank in a state dispersed in the culture solution. Can do. Moreover, when using mycelium solid-cultured as an inoculum, it can be carried out by putting an appropriate amount directly into a tank in a clean room.

  In the method for producing a mycelium culture extract of the present invention, the solid medium inoculated with the mycelium is agitated by applying repeated movement of falling and rising to the solid medium.

  Stirring can be performed, for example, by putting the solid medium inoculated with the mycelium of basidiomycetes as described above into a tank-like culture container and rotating it, and transmitting the rotational force to the solid medium. it can. In this case, in order to spread the basidiomycete attachment site throughout the solid medium efficiently, the ratio of the solid medium to the culture vessel volume is preferably 5 to 80%, and preferably 10 to 50%. More preferred. In addition, as for the amount of solid medium filled into the culture vessel, it is only necessary to secure the space required for the stirring, so that the culture can be easily scaled up only by changing the volume of the culture vessel.

  The culture vessel is preferably made of stainless steel, but it is possible to keep a hermetic state during the culture period and to have a ventilating pipe or the like so that appropriate ventilation can be performed. preferable. On the other hand, as long as these conditions are satisfied, inexpensive equipment such as a drum can be used.

  There is no particular limitation on the method of stirring by applying a repeated motion of falling and rising to the solid medium, and when rotating a tank-like culture vessel, using a dedicated turntable or the like, it is about 5-30 rpm. What is necessary is just to rotate by rotation speed. In addition to the method using rotation, for example, a method such as a method of moving up and down by a forklift or the like in which a drum clipper is mounted on a drum-like culture vessel can be used. Furthermore, it is also possible to insert a stirring rod aseptically and stir from the inside. When stirring, a baffle plate (so-called baffle plate) can be attached to the inner wall of the tank-like culture vessel to increase the stirring efficiency.

  After completion of the stirring for allowing the basidiomycete attachment site to spread throughout the solid medium, the basidiomycete is maintained and cultured in a culture environment in which the mycelium of the basidiomycete grows. For example, the cells are cultured for about 50 days in a culture room whose temperature is controlled at 18 to 25 ° C. During the culture period, the culture vessel is allowed to stand or cultured with appropriate rotation. Moreover, you may culture | cultivate suitably aeration. In this way, a mycelium culture in which mycelium is prevalent can be obtained.

After completion of the culture, a culture extract is obtained from the obtained culture. At that time, the mycelium is preferably self-digested and extracted using an enzyme present in the mycelium. As a preferable example of the method, the mycelium culture after culturing is crushed, a small amount of water is added as necessary, the mixture is treated at 30 to 60 ° C. for 3 to 6 hours, and the mycelium enzyme reaction is advanced. Let yourself digest. Subsequently, this crushed material is infiltrated with warm water or hot water of 50 ° C. or higher, and an active ingredient is extracted. The extraction can also be carried out under a high pressure such as 120 ° C. under a vapor pressure of 1.2 kg / cm ² , for example.

  In addition, the culture | cultivation after culture | cultivation may crush in a culture container, without taking out a culture medium from a culture container, and may transfer to an extraction process as it is.

  The extracted suspension thus obtained is filtered or centrifuged, and the filtrate or supernatant is collected to obtain a mycelium containing a degradation product of a medium, a mycelium metabolite, a mycelium cell degradation product, and the like. A culture extract can be obtained. The mycelium culture extract obtained by the production method of the present invention contains components equivalent to those obtained by the conventional production method, as shown in the following examples.

  On the other hand, the mycelium culturing apparatus of the present invention is a culture container having an inlet for introducing a solid medium and mycelia of basidiomycetes, a lid for sealing the inlet, and a vent for circulating air. And rotating means for rotating the culture vessel with a horizontal or inclined rotating shaft.

  Hereinafter, the mycelium culture apparatus of the present invention will be specifically described with reference to the drawings.

  1 and 2 show an example of the mycelium culture device of the present invention.

  This mycelium culture apparatus 10 includes an inlet 21 for introducing a solid medium and mycelium of basidiomycetes, a lid 22 for sealing the inlet 21, and a vent hole 23 for circulating air. A substantially cylindrical culture vessel 20 is provided. Also, a support base 30 that supports the culture vessel 20 so as to be removable and rotatable, a plurality of rotating rollers 40 that are provided on the support base 30 and that are in contact with the side surfaces of the culture vessel 20 to support the culture vessel 20; And a driving device 50 that rotates at least one of the rotating rollers that are in rolling contact with the side surface of the culture vessel. The support base 30, the plurality of rotating rollers 40, and the driving device 50 constitute a rotating means 60 that rotates the culture vessel 20 as a whole.

  Specifically, the support base 30 includes a lower surface plate 31 and an upper surface plate 33 supported on the lower surface plate 31 via support legs 32 and inclined with respect to a horizontal plane. Is provided with a roller bearing 40a, on which a roller shaft 40b with a rotating roller 40 is rotatably supported. A first pulley 45 is provided on at least one end of the roller shaft 40b, and rotational driving force is transmitted to the second pulley 46 provided on the driving device 50 side via a belt 70. It is configured as follows. Then, when the rotational driving force by the driving device 50 is applied to the roller shaft 40b, the rotational driving force is transmitted to the side surface of the substantially cylindrical culture vessel 20 in contact with the rotational roller 40, and the rotational center A is pivoted. Thus, the culture vessel 20 is rotated in a state substantially parallel to the roller shaft 40b.

  Further, a roller bearing 41a is disposed on the bottom surface side of the culture vessel 20 of the upper surface plate 33 (on the side of the vent hole 23 and the desorption nozzle 24), and a rotating roller is provided on the roller shaft 41b erected on the roller bearing 41a. 41 is rotatably supported. The rotating roller 41 is in contact with the bottom surface of the culture vessel 20 and rotatably supports the bottom surface of the culture vessel 20 so that the culture vessel 20 is not displaced along the inclined upper surface plate 33.

  The culture vessel 20 is provided with a desorption nozzle 24 on the outside air side of the vent hole 23, and a tubular vent tube 25 is further attached to the desorption nozzle.

  By putting the solid medium and the mycelium of basidiomycetes into the culture container of the mycelium culture device configured in this way and applying rotation, the centrifugal force and the weight of the medium filled in the culture container interact with each other. Thus, repeated movements of falling and rising occur, and stirring is performed. After stirring in this manner, the culture vessel 20 is removed from the support base 30 and placed in a culture room in an environment suitable for culture to perform culture. When culturing, air or the like sterilized by a filter can be fed from an aeration tube attached to the vent hole as necessary. In addition, you may culture | cultivate, stirring regularly while putting the culture container 20 on the support stand 30. FIG.

  In the mycelium culture apparatus of the present invention, the material of the culture vessel is not particularly limited, but is preferably a material that is durable to repeated autoclaving treatment such as stainless steel.

  The mycelium culture apparatus of the present invention can be suitably used in the above-described method for producing the mycelium culture extract of the present invention, and scale-up can be performed only by changing the capacity of the culture vessel.

  EXAMPLES Hereinafter, the present invention will be specifically described with reference to examples, but these examples do not limit the scope of the present invention.

<Example 1>
Mix 90% bagasse and 10% defatted rice bran, adjust the water content to 70%, create a solid medium, fill 48kg into a 200 liter stainless steel tank, and then autoclave according to conventional methods did. This was inoculated with mannen mycelium. And using the rotation apparatus which has the structure similar to the rotation means of the mycelium culture apparatus shown in the said FIG. 1, it rotated for 30 rpm and 30 minutes, and stirred the culture medium and the mycelium in a tank. The tank was transferred to a culture room adjusted to 25 ° C. and left to stand for 51 days in the culture room, and then the medium in which mycelium was infested was crushed to a size of a thumb with a crusher. The crushed medium was treated at 40 ° C. for 6 hours to promote autolysis, then packed in an extraction tank, and extracted for 16 hours while circulating hot water at 60 ° C.

<Test Example 1>
The properties of the extract obtained in Example 1 were compared with the properties of the extract obtained by the conventional method. In the “conventional method” to be compared in this example, the following methods are used as the steps of “medium filling / sterilization” and “inoculation of inoculum” in each example including the following examples. It was.
[Conventional method]
-“Medium filling / sterilization” step: 800 g of solid medium per bag is packed in a polypropylene bag and sterilized at high temperature under pressure.
Step of “inoculation with inoculum”: The mycelium is injected from the inoculation port provided above the solid medium after filling and sterilization.

  In the conventional method, a culture period of about 3 months (97 days in Table 1) was required to obtain an extract with a low pH and a high solid content (extract with high maturity). According to the method 1, an extract with equivalent properties could be obtained in about half the culture period (51 days). Moreover, in the conventional method, it was confirmed that the pH of the extract obtained in the same culture period (55 days) as in the method of Example 1 was high and the solid content% was low. From these results, it became clear that according to the present invention, a mycelium culture extract having a high maturation degree can be obtained in about half the conventional culture period.

<Example 2>
Mannen koji mycelium was cultured in the same manner as in Example 1, and the same treatment was performed to obtain an extract. The obtained extract was filtered through a cartridge filter, further sterilized by filtration through a membrane filter, concentrated, and freeze-dried to obtain a brown powder. The results of analyzing the components of this powder and comparing it with the powder obtained from the conventional culture were as follows.

  As apparent from Table 2, the powder obtained by the method of Example 2 (culture period 50 days) has a chemical composition equivalent to that obtained by the conventional method (culture period 3 months). It was judged. Therefore, it is the same as the culture extract (powder) obtained by the conventional method in which an increase in blood glucose level (Patent Publication No. 2005-213211) and an antihypertensive action (Patent Publication No. 2006-265179) are found. It was suggested to have an effect.

<Example 3>
Mix 90% bagasse and 10% defatted rice bran, adjust the water content to 80%, make a solid medium, fill 7.2kg into a 60 liter stainless steel tank, and then use high pressure steam as usual Sterilized. This is inoculated with mannen mycelia and rotated at 30 rpm for 30 minutes using a rotating device having the same structure as the rotating means of the mycelium culture device shown in FIG. Was stirred. The tank was transferred to a culture room adjusted to 25 ° C. and left to stand for 48 days in the culture room. Appropriate amounts were collected from the upper, central and lower parts of the medium in which mycelia were infested. Extracts and powders were prepared, and the properties and components were compared for each.

  From Tables 3 and 4, there was no difference in the degree of maturation and the obtained culture extract (powder) component depending on the part of the culture medium. In the method of the present invention, unlike the conventional method, the mycelium and the medium are agitated in advance so that the inoculated site of the basidiomycete spreads uniformly throughout the solid medium, and the growth of the mycelium and the decomposition / equilibrium of the medium components This was thought to be due to the fact that the chemicalization proceeds simultaneously regardless of the site.

<Example 4>
Mix 90% bagasse and 10% defatted rice bran, adjust the water content to 80%, make a solid medium, fill 7.2kg into a 60 liter stainless steel tank, and then use high pressure steam as usual Sterilized. This was inoculated with mycelia of shiitake mushrooms and rotated at 30 rpm for 30 minutes using a rotating device having the same structure as the rotating means of the mycelium culture device shown in FIG. Stir. The tank was transferred to a culture chamber adjusted to 21 ° C., and allowed to stand for 51 days in the culture chamber. After the mycelium had spread in the medium, a culture extract was obtained in the same manner as in Example 1 and Example 2. . The properties (ripening level) of the obtained extract were similar to those obtained by culturing for 4 months by the conventional method. The component composition of the culture extract (powder) was also the same as that obtained by the conventional method.

  Therefore, according to the present invention, it has been clarified that the effect of shortening the culture / maturation period can be obtained when producing the mycelium culture extract of shiitake mushroom as well as the mannen moth.

It is a perspective view showing the mycelium culture apparatus of one embodiment of the present invention. FIG. 2 is a perspective view illustrating a mycelium culture apparatus according to an embodiment of the present invention from a direction different from that in FIG.

Explanation of symbols

DESCRIPTION OF SYMBOLS 10 Mycelium culture apparatus 20 Culture container 21 Input port 22 Lid 23 Vent hole 24 Desorption nozzle 25 Vent pipe 30 Support base 31 Bottom plate 32 Support leg 33 Upper plate 40, 41 Rotating roller 40a, 41a Roller bearing 40b, 41b Roller shaft 45 First pulley 46 Second pulley 50 Driving device
60 Rotating means 70 Belt

Claims (10)

  A solid medium containing plant fiber raw material is inoculated with basidiomycetes mycelium, and the solid medium inoculated with the mycelium is stirred by applying repeated movement of falling and rising to the solid medium, or stirred. On the other hand, a method for producing a mycelium culture extract, wherein the mycelium is maintained in a culture environment where the mycelium grows, and a culture extract is obtained from the obtained culture.   The method for producing a mycelium culture extract according to claim 1, wherein the solid culture medium inoculated with the mycelium is placed in a culture container, and the culture container is rotated to impart repeated movement of falling and rising to the solid culture medium.   The method for producing a mycelium culture extract according to claim 1 or 2, wherein the plant fiber raw material is prepared from a plant of the genus Aceae.   The plant fiber raw material is prepared from one or more selected from bagasse, corn stover, wheat bran, rice bran, rice straw, rice husk, straw, bear cocoon, and bamboo. 4. The method for producing a mycelium culture extract according to any one of 3 above.   The basidiomycete is selected from Mannen mushrooms, Bukuryo, Kofukisarokoshikake, Kawara mushrooms, Shiitake mushrooms, Hira mushrooms, Mai mushrooms, Enoki mushrooms, Shimeji mushrooms, Yamabushi mushrooms, and Agaricus. The manufacturing method of the mycelium culture extract as described in one.   A culture container having an inlet for charging a solid medium and mycelium of basidiomycetes, a lid for sealing the inlet, and a vent hole for circulating air, and rotating the culture container horizontally or inclined A mycelium culture device characterized by comprising a rotating means for rotating the shaft.   The rotating means includes a support base that detachably and rotatably supports the culture vessel, a plurality of rotary rollers that are provided on the support base and that are in contact with a side surface of the culture vessel and support the culture vessel; The mycelium culture device according to claim 6, comprising: a driving device that rotates at least one of the rotating rollers that are in rolling contact with a side surface of the container.   The mycelium culture apparatus according to claim 6 or 7, wherein the culture container is a substantially cylindrical culture container.   The mycelium according to any one of claims 6 to 8, wherein a desorption nozzle is provided on the outside air side of the vent of the culture container, and a tubular vent tube is further attached to the desorption nozzle. Culture device.   The mycelium culture apparatus according to any one of claims 6 to 9, wherein the culture container is a culture container capable of being subjected to autoclaving treatment.

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