JP2009057312A - Antioxidant and cosmetic containing the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an antioxidant having a high antioxidation effect and a cosmetic containing the antioxidant, exhibiting the potent preventing and improving effects of skin roughness, also having less irritation to the skin and excellent in safety. <P>SOLUTION: This antioxidant contains an extract obtained by extracting from at least one of a plant selected from Phyllanthus emblica, Sapindus trifoliatus and Acacia concinna with electrolytically reduced water. The cosmetic containing the antioxidant is also provided. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to an anti-oxidant that is hypoallergenic to the skin and has a high anti-oxidant effect and rough skin prevention / improving effect, as well as makeup such as basic cosmetics, make-up cosmetics, and hair cosmetics containing the antioxidants. Regarding fees.

Recent studies have shown that active oxygen is generated when the skin is exposed to ultraviolet light. Active oxygen peroxidizes sebum and damages skin cells. And it reduces the function of the cell, causing the inhibition of normal keratinization and the production of extracellular matrix such as collagen and hyaluronic acid. Furthermore, it promotes the degradation and crosslinking of extracellular matrices such as collagen and hyaluronic acid, resulting in the skin becoming inflexible and elastic, causing wrinkles, tarmi and rough skin.
In order to prevent and improve skin aging due to such oxidation, various antioxidants have been searched for and studied. Examples of such antioxidants include tocopherol (vitamin E), ascorbic acid (vitamin C), butylhydroxyanisole (BHA), butylhydroxytoluene (BHT), sesamol dimer, asteraceae Examples thereof include plant extracts and extracts of the plant belonging to the genus Sarogase.

  As described above, various antioxidants have been developed so far, but synthetic antioxidants have safety problems, the amount of addition is limited, and natural antioxidants are effective. Therefore, safer and more effective antioxidants and cosmetics containing the same are desired.

As a result of diligent research to solve the above-mentioned problems, the present inventor has found a strong antioxidant effect on extracts extracted from a solvent containing electroreduced water from Ammarok, Sapinporia trifoliatus and Acacia Consina, Furthermore, because it has been confirmed to be highly safe, it contains an antioxidant with excellent efficacy and safety, and a cosmetic that exhibits an excellent effect in preventing and improving the symptoms of skin aging. Found to provide.
Furthermore, the cosmetics used in this specification include quasi-drugs in addition to cosmetics.

Regarding the above-mentioned three kinds of plants used in the present invention, with respect to Ammarok, it is characterized in that ethanol tincture from leaves, skins and fruits is used for the effectiveness in the skin and the past results in cosmetics. Cosmetics (Japanese Translation of PCT International Publication No. 02-504638), lipid peroxide production inhibitor comprising an extract of water, alcohol and the like from fruits (JP 04-069343), an antioxidant containing an extract from fruits and roots, A composition containing the antioxidant (Japanese Patent Laid-Open No. 2002-249772), an external preparation for skin containing an extract from pericarp, leaves and the like, and an active oxygen scavenger (Japanese Patent Laid-Open No. 2003) -081749).
With regard to sapin moth strobiolius and deer silkworm, there is a combined use with mulberry extract (Japanese Patent Laid-Open No. 2005-239715).
However, an extract obtained by mixing three or more of the above-mentioned three kinds of plants with two or more kinds and extracted with electroreduced water exhibits a stronger antioxidant effect than conventional extracts, and is prominent in preventing and improving rough skin even in humans. It has not been known at all to show the effect, and will be revealed for the first time with the present invention.

JP 2001-139944 A JP-A-11-180886 Japanese Patent Laid-Open No. 10-182413 Japanese National Patent Publication No. 02-504638 Japanese Patent Laid-Open No. 04-069343 JP 2002-249772 A JP 2003-081749 A JP 2005-239715 A

  As described above, the present invention has a strong antioxidant effect as compared with conventional extracts of amarok, sapinporia trifoliatus and / or acacia concina extracted with water, alcohol, organic solvent, etc., and A highly safe anti-oxidant containing electrolytically reduced water extract as an active ingredient and the anti-oxidant added to cosmetics to prevent and improve wrinkles, tarmi and rough skin, Can be provided.

The three types of plants used in the present invention will be described.
Ammarok (also known as Amla) is a small fruit tree native to Southeast Asia with the scientific name Phyllanthus emblica, distributed from Pakistan to Indonesia. In this plant, fruit is used as an edible or mordant as a tonic, bark is used as a dye, and a liquid decocted with leaves is used for washing eyes.
Sapin ヅ strihoriatus (aka Rita) is a tree whose scientific name is Sapindus trifoliatus and is distributed from India to China and Southeast Asia. This plant is said to have an effect of preventing hyperemia and pain when the eyes are washed with a liquid decocted as a tonic, hemostatic and expectorant skin.
Acacia concina (also known as Sikakai) is a tree whose scientific name is Acacia concinna and is distributed in India and the like.
Electrolytically reduced water is water with high osmotic pressure having reducibility that is generated on the cathode side of the electrolytic cell by electrolyzing water. There are cases where it has been drunk for application to artificial dialysis or for the improvement of atopic dermatitis, but there is no knowledge of application to the skin.

  The part used for extraction is a part of the plant body such as leaves, stems, bark, flowers, fruits, roots or the whole plant, and preferably, fruits are used.

Preparation of the extract was carried out by removing the foreign material, if necessary, after it had been removed or crushed as needed, and then minced or pulverized as necessary. It can carry out by making it contact with electrolytic reduction water according to conventional methods, such as a distribution method.
The ratio of each plant body and the extraction solvent is not particularly limited, but it is preferable to extract with 5 to 50 times the amount of solvent relative to the weight of the plant body. In addition, when the above two types or three types of plants are mixed and extracted, the mixing ratio is not particularly limited, but it is preferable to mix them in equal amounts.

  The extraction solvent is electrolytic reduced water, but water, alcohols (for example, lower alcohols such as methanol, absolute ethanol, ethanol, or polyhydric alcohols such as propylene glycol and 1,3-butylene glycol) or ketones such as acetone. Further, esters such as ethyl ether, dioxane, acetonitrile, and ethyl acetate, and organic solvents such as xylene, benzene, and chloroform can be used alone or in combination of two or more kinds with electrolytically reduced water. It can also be used in the state where each solvent extract is combined.

  Incidentally, the production method is not particularly limited, but in the case of the immersion method, it is usually sufficient to be in the range of the boiling point from the melting point of the solvent at room temperature and normal pressure, and after extraction, filtration or ion exchange resin is used, Adsorption, decolorization, and purification may be used to form a solution, paste, gel, or powder. If necessary, further purification treatments such as deodorization and decolorization may be added as long as the effect is not affected. As a purification treatment means such as deodorization and decolorization, an activated carbon column or the like may be used. The usual means to be applied can be arbitrarily selected.

Further, the pH of the extract is not particularly limited, but it is preferably mainly in the range of 2 to 8, and citric acid, sodium citrate, sodium hydroxide, hydrochloric acid, phosphoric acid, etc. are used as a pH adjuster. Also good.
Furthermore, if necessary, it may be used as an appropriate concentration by concentration under reduced pressure or the like, and in some cases, it can be pulverized according to a conventional method such as spray drying or freeze drying.

  The cosmetics containing the extract of Ammarok, Sapinporia trichoriatus, Acacia concina of the present invention specifically refers to skin cosmetics and hair cosmetics, more specifically lotions, emulsions, Cream, oil, ointment, pack, lip, lipstick, foundation, eyeliner, blusher, mascara, eye shadow, nail polish / pedicure, nail coating remover, shaving agent, shampoo, rinse, hair treatment, Hair tonics, hair sprays, hair creams, hair lotions, hair straighteners, hair growers, permanent liquids, hair dyes, hand soaps, body soaps, toothpastes, mouth washes, facial cleansers, soaps, etc. It is not limited.

  The amount of the extract of ammarok, sapin coconut trifoliatus and acacia concina blended in the cosmetics of the present invention is not particularly limited, but when used alone or in combination, generally it is a dry solid relative to the total amount of the cosmetics. The total amount is 0.0001 to 10.0% by weight, preferably 0.0005 to 5.0% by weight, particularly preferably 0.001 to 3.0% by weight.

  The cosmetics of the present invention are oily raw materials, surfactants, moisturizers, thickener polymers, as long as they do not impair the effects of the present invention, in addition to the extract of Amarok, Sapinporia trifoliatus, Acacia concina. A film polymer, an ultraviolet absorber, an antioxidant, an antibacterial agent, a sequestering agent, a powder component, a physiologically active component, and the like can be blended and produced in any amount alone or in combination.

  Examples of the physiologically active component include ascorbic acid derivatives, hydroquinone derivatives, Alanium salvifolium (Linn. F.) Wang extract, Pertophorum dashirachis (Miq.) Kurz ex Baker And Pithecellobium tenu crib extract and the like.

  The present invention will be described more specifically with reference to the following examples, test examples and formulation examples, but the present invention is not limited thereto.

Example 1
20 g of each dried product of each of the fruits of Ammarok, Sapin ヅ trifoliatus and Acacia Concina was extracted with 600 ml of electrolytic reduced water at 5 ° C. for 18 hours. The obtained extract was filtered to obtain 491.3 g of a brown extract (evaporation residue: 1.92%).
Example 2
20 g of each dried product of each of the fruits of Ammarok, Sapinporia tricholiatus and Acacia Concina was extracted with 600 ml of electrolytic reduced water containing 50% 1,3-butylene glycol at room temperature for 1 week. The obtained extract was filtered to obtain 521.8 g of a brown extract (evaporation residue: 2.81%).
Comparative Example 1
20 g of each dried product of each of the fruits of Ammarok, Sapin ヅ trifoliatus and Acacia Concina was extracted with 600 ml of purified water containing 50% 1,3-butylene glycol at room temperature for 1 week. The obtained extract was filtered to obtain 491.4 g of a brown extract (evaporation residue: 3.02%).

Test example 1
Evaluation of free radical scavenging activity Using 1,1-diphenyl-2-picrylhydrazyl (DPPH), which is a stable organic radical, the mixed electroreduced water extract of the amarok fruit, sapin strawberry triphoria fruit, and acacia consina fruit of the present invention The free radical scavenging action of was investigated.
〔Test method〕
3 ml of 0.5 mM 1,1-diphenyl-2-picryhydrozyl (DPPH) solution (50% ethanol-0.1 M acetate buffer (pH 5.5)) was added to 3 mL of Ammarok fruit and Sapin ヅ trifoliatus fruit of Example 2. -Acacia Consina fruit mixed electrolytic reduced water extract, Ammarok fruit of Comparative Example 1-Sapin ヅ strihoriatus fruit-Acacia Concina fruit mixed water extract or 0.3 ml of electrolytic reduced water were added at 37 ° C for 10 minutes. Absorbance at 517 nm was measured after incubation. As a positive control, a case where a 0.1% α-tocopherol solution was added instead of the extract of Example 1 and a case where a sample was not added as a blank were also carried out.
The relative value of the absorbance of each sample with respect to the absorbance of the blank was determined and used as the radical residual rate (%).

〔result〕
The results are shown in Table 1.

From the results shown in Table 1, the mixed electroreduced water extract of Ammarok fruit, Sapin ヅ strihoriatus fruit, and Acacia concina fruits of the present invention is compared with the extract of Ammarok fruit, Sapin ヅ strihoriatus fruit, and Acacia concina fruits mixed water. It can be seen that it has a high radical scavenging effect. Further, it can be seen that this effect is not due to the electrolytically reduced water itself, but to the antioxidant component extracted by the electrolytically reduced water.
In addition, since α-tocopherol used as a positive control also markedly erased radicals, it can be said that the test system is normal.

Cosmetics shown in Formulation Examples 1 to 11 were prepared by a conventional method.

Formulation Example 1 Cream The following component A and separately the following component B are heated and dissolved at 75 ° C. to obtain A solution and B solution, respectively. Liquid B was added to liquid A, emulsified, cooled to 50 ° C. with stirring, component C was added, and a cream was prepared.
Component A
Jojoba oil 3.0% by weight
Squalane 2.0% by weight
Methyl polysiloxane 0.5% by weight
Stearyl alcohol 0.5% by weight
Cetyl alcohol 0.5% by weight
Tri (capryl / capric acid) glyceryl 12.5% by weight
Glyceryl monostearate 5.0% by weight
Diglyceryl monostearate 1.5% by weight
Decaglyceryl monostearate 3.0% by weight
Propyl paraoxybenzoate 0.1% by weight
Component B
Xanthan gum 0.1% by weight
Mixed electroreduced water extract 10.0% by weight of Ammarok fruit, Sapin strawberry trifoliatus fruit and Acacia concina fruit of Example 1
Glycerin 1.0% by weight
1,3-butylene glycol 5.0% by weight
Methyl paraoxybenzoate 0.2% by weight
Purified water 62.0% by weight
Component C
Fragrance 0.1% by weight

Formulation Example 2 Toner lotion The following component A was mixed and dissolved to prepare a solution A. Separately, the following component B was mixed and dissolved to prepare a solution B, and the solution A and the solution B were mixed uniformly to prepare a lotion.
Component A
Polyoxyethylene sorbitan laurate 1.2% by weight
Ethyl alcohol 5.0% by weight
Methyl paraoxybenzoate 0.2% by weight
Fragrance 0.1% by weight
Component B
Quinceaide extract 8.0 wt%
Glycerin 3.0% by weight
1,3-butylene glycol 5.0% by weight
Example 2: Amarok fruit, sapin strawberry trihorias fruit, acacia concina fruit mixed electrolytic reduced water extract 10.0% by weight
75.5% by weight of purified water

Formulation Example 3 Emulsion The following component A and separately component B are heated and dissolved at 75 ° C. to form A and B solutions, respectively. In addition, an emulsion was prepared.
Component A
Jojoba oil 1.0% by weight
Squalane 2.0% by weight
Behenyl alcohol 1.0% by weight
Tri (capryl / capric acid) glyceryl 2.0% by weight
Tetraglycerin condensed silinoleic acid 0.1% by weight
Propylene glycol monooleate 0.5% by weight
1.0% by weight of glyceryl monostearate
Monoglycerin hexaglycerin 1.0% by weight
Monomyristic acid decaglycerin 0.5% by weight
Propyl paraoxybenzoate 0.1% by weight
Component B
Quince essence extract 5.0% by weight
Jojoba leaf extract 2.0% by weight
Example 2: Amarok fruit, sapin strawberry trihorias fruit, acacia concina fruit mixed electrolytic reduced water extract 10.0% by weight
1,3-butylene glycol 3.0% by weight
78.7% by weight of purified water
Component C
Fragrance 0.1% by weight

Formulation Example 4 Soap was produced by mixing the following ingredients according to a conventional method for producing soap.
Ingredients soap base 53.2% by weight
Scroll 19.4% by weight
Jojoba oil 0.25% by weight
Mixed electroreduced water extract 10.0% by weight of the amarok fruit, sapin strawberry trihoriatus fruit and acacia concina fruit of Example 1
Concentrated glycerin 6.5% by weight
Hydroxyethanediphosphonic acid 0.15% by weight
Normal water 18.0% by weight

Formulation Example 5 Cleansing Gel The following components A and B are heated and dissolved at 70 ° C. to prepare A solution and B solution, respectively, and B solution is added to A solution and stirred until uniform. Cooling to 50 ° C. with stirring, component C was added to prepare a cleansing gel.
Component A
Hexaglyceryl monomyristate 20.0% by weight
Liquid paraffin 58.8% by weight
P-Hydroxybenzoate 0.3% by weight
Component B
Mixed electroreduced water extract 10.0% by weight of the amarok fruit, sapin strawberry trihoriatus fruit and acacia concina fruit of Example 1
Concentrated glycerin 5.9% by weight
Sorbitol 5.0% by weight
Purified water 9.4% by weight
Component C
Fragrance 0.1% by weight

Formulation Example 6 Packing agent A phase, B phase and C phase were each uniformly dissolved, B phase was added to A phase to solubilize, then C phase was added and uniformly dissolved to prepare a packing agent.
Phase A dipropylene glycol 5.0% by weight
Polyoxyethylene hydrogenated castor oil 5.0% by weight
Phase B olive oil 5.0% by weight
Tocophenol acetate 0.2% by weight
P-Hydroxybenzoate 0.2% by weight
Phase C sodium bisulfite 0.03% by weight
Polyvinyl alcohol 13.0% by weight
Mixed electroreduced water extract 10.0% by weight of the amarok fruit, sapin strawberry trihoriatus fruit and acacia concina fruit of Example 1
Jojoba leaf extract 1.0% by weight
Ethanol 7.0% by weight
Purified water 62.77% by weight

Formulation Example 7 Emulsion-type foundation A powder part A in which the following component A is sufficiently mixed and ground is used, component B is liquid B, and component C is liquid C. After the liquid C is heated and stirred, A is added and subjected to a homomixer treatment, and the liquid B which has been heated and mixed is further added to perform the homomixer treatment. While stirring, the mixture was cooled to 50 ° C., component D was added, and the mixture was further cooled to room temperature to prepare an emulsified foundation.
Component A
Titanium dioxide 10.3% by weight
Sericite 5.4 wt%
Kaolin 3.0% by weight
Yellow iron oxide 0.7% by weight
Bengala 0.4% by weight
Black iron oxide 0.2% by weight
Component B
Decamethylcyclopentasiloxane 11.5% by weight
Liquid paraffin 8.5% by weight
Component C
Sorbitan sesquioleate 3.0% by weight
1.5% by weight of an electroreduced water extract of the mixture of Ammarok fruit, Sapin strawberry trifoliatus fruit and Acacia concina fruit of Example 1
1,3-butylene glycol 5.0% by weight
P-Hydroxybenzoate 0.2% by weight
Purified water 50.1% by weight
Component D
Fragrance 0.2% by weight

Formulation Example 8 Solid Foundation The following component A was uniformly mixed with a blender, component B was added thereto, and kneaded well to prepare a solid foundation.
Component A
Talc 42.4% by weight
Kaolin 15.5% by weight
Sericite 10.0% by weight
Zinc flower 7.0% by weight
Titanium dioxide 3.8% by weight
2.9% by weight of yellow iron oxide
Black iron oxide 0.2% by weight
Component B
Squalane 8.0 wt%
Isostearic acid 4.0% by weight
Polyoxyethylene sorbitan monooleate 3.0% by weight
Isocetyl octoate 2.0% by weight
1.0% by weight of an electroreduced water extract of the mixture of Ammarok fruit, Sapin strawberry trifoliatus fruit and Acacia concina fruit of Example 1
Paraoxybenzoate 0.1% by weight
Fragrance 0.1% by weight

Formulation Example 9 Hair-Tonic After component B was added to component A below and dissolved by stirring, component C was added and further stirred to prepare a hair-tonic.
Component A
Ethanol 50.0% by weight
Component B
Glycerin 3.0% by weight
L-menthol 0.1% by weight
Assembly extract 2.0% by weight
Example 2: Amarok fruit, sapin strawberry trihorias fruit, acacia concina fruit mixed electrolytic reduced water extract 10.0% by weight
Fragrance 0.2% by weight
Component C
Ginseng extract 3.0% by weight
Purified water 36.7% by weight

Formulation Example 10 Shampoo
The following components were heated and mixed uniformly to prepare a shampoo.
Component N-coconut oil fatty acid glutamic acid triethanolamine 25.0% by weight
Lauric acid diethylethanolamide 5.0% by weight
Potassium myristate 5.0% by weight
2.0% by weight of ethylene glycol distearate
Polyethylene glycol 400 15.0% by weight
Jojoba oil 1.0% by weight
Example 2: Amarok fruit, sapin strawberry trihorias fruit, acacia concina fruit mixed electrolytic reduced water extract 10.0% by weight
Chloroxylenol 0.1% by weight
Vitamin E 0.1% by weight
P-Hydroxybenzoate 0.2% by weight
Fragrance 0.3% by weight
Purified water 43.3% by weight

Formulation Example 11 Bath Agent A bath agent was prepared in the usual manner using the following components.
Ingredient dry sodium sulfate 40.0% by weight
Sodium bicarbonate 57.5% by weight
Olive oil 0.2% by weight
0.1% by weight of an electrolytically reduced water extract of the mixture of Ammarok fruit, Sapin strawberry trifoliatus fruit and Acacia concina fruit of Example 1
Light anhydrous silicic acid 0.3% by weight
Fragrance 1.7% by weight
Yellow No. 202 (1) 0.2% by weight

Comparative Formulation Example 1 Lotion Toothpaste Amaroc fruit and sapin koji of Comparative Example 1 containing only 10.0 wt. A skin lotion was prepared in place of 10.0% by weight of the mixed water extract of Strifoliatus fruit and Acacia concina fruit.

Control Example Skin Lotion A lotion was prepared by changing only 10.0% by weight of the mixed electroreduced water extract of Ammarok fruit, sapin ヅ trifoliatus fruit, and acacia consina fruit of Example 2 in Formulation Example 2 to purified water. .

Test Example 2 Skin Roughness Improvement Test For each lotion of Formulation Example 1, Comparative Formulation Example 2 and Control Example, the degree of improvement against skin roughness was confirmed by a monitor test.
〔Test method〕
Appropriate amount of each cosmetic was applied once a day in the morning and evening for 15 weeks with 15 males and females 20- to 40-year-old who had consented to the document as subjects, and the inner part of the left and right upper arms as a test zone. This state was visually determined based on the following criteria.

[Criteria]
Effective: Clear improvement in rough skin was observed compared to control example Somewhat effective: Improvement in rough skin was observed compared to control example No effect: Almost the same level as in control example

The results are shown in Table 2.

  As is apparent from Table 2, the cosmetic (formulation example 2) containing the electrolytically reduced water extract of the mixture of ammarok, mkuroji, and acacia concina as compared with Comparative Formulation Example 1 has an excellent effect of improving rough skin. Admitted.

Test Example 3 Skin irritation [Sample]
Product of the present invention: A mixed electroreduced water extract of Ammarok fruit, Sapin strawberry trifoliatus fruit, and Acacia concina fruit of Example 2 in JP JP hydrophilic petrolatum so as to be 10% by weight.
Blank: JP Hydrophilic petrolatum [Test method].
The sebum was removed from the upper arm inner part of 10 adult men and women who had given consent in writing with ethanol to make a test section. A filter paper in which 0.2 g of each sample was immersed in the fin chamber was placed, and an occlusion patch was applied to the test area for 24 hours. Thereafter, the fin chamber was removed, and after 1 hour, 24 hours, and 48 hours after removal, the degree of erythema / edema at the patch site was visually determined based on the following “skin irritation criteria by dreze method”.
[Criteria]
(Erythema)
Score 0: No erythema Score 1: Very mild erythema Score 2: Obvious erythema Score 3: Moderate to strong erythema Score 4: Light crust formation in strong crimson erythema

(edema)
Score 0: No edema Score 1: Very mild edema Score 2: Clear edema (clearly distinguishable from surroundings)
Score 3: moderate edema (swelling of 1 mm or more)
Score 4: Strong edema (and spread around)

The results are shown in Table 3.

As is apparent from the results in Table 3, it can be seen that the extract has no irritation to the skin and is extremely safe.

Claims (4)

  An antioxidant comprising, as an active ingredient, an extract extracted from at least one kind of plant selected from Amarylok (Phyllanthus emblica), Sapindu trifoliatus, and Acacia concinna (Acacia concinna).   An antioxidant containing as an active ingredient an extract extracted from three kinds of plants, Ammaroc (Phyllanthus emblica), Sapindu trifoliatus and Acacia concinna.   An antioxidant comprising as an active ingredient an extract extracted from the fruits of three kinds of plants, Ammaroc (Phyllanthus emblica), Sapins trifoliatus (Sapindus trifoliatus) and Acacia concinna (Acacia concinna).   A cosmetic comprising a total of 0.0001 to 10.0 wt% of the antioxidant according to any one of claims 1 to 2 in terms of dry solid content.