JP2008248043A - Allergen inactivator and allergen inactivating product comprising the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an allergen inactivator inactivating allergens by strongly capturing allergen proteins and to provide a cosmetic or an allergen inactivating product prepared by formulating the allergen inactivator. <P>SOLUTION: The allergen inactivator strongly capturing the allergen proteins and thereby inactivating the allergens can be provided by including one or two or more of silica having ≤150 μm particle size, particulate zinc oxide and zeolite as the allergen inactivator and a humectant having a high moisture retaining ability used in combination therewith. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

The present invention relates to a powder material that inactivates an allergen, and further relates to a cosmetic or an allergen inactivation product that contains the powder material.

At present, it is said that there are more than 20 million patients with hay fever all over Japan, and about one out of every five Japanese people is said to have hay fever. The background of the increase in the number of patients is thought to be an increase in the amount of pollen, which is an allergen, and changes in the living environment.

The background of the increase in the amount of pollen scattering can be attributed to post-war Japanese plantations. The cedar planted from 1958 to 1947 accounted for 47% of the total cedar plantation. It is thought that the amount of scattering increased because cedar actively produced pollen after about 30 years.

One way to avoid scattered pollen is to avoid direct contact with the use of a mask or glasses, but it is virtually impossible to avoid contact completely, so detoxification of the pollen itself is most desirable for hay fever. It seems to be a solution.

Conventionally, various techniques for inactivating pollen allergens have been proposed. For example, pollen allergen inactivation spray (see Patent Document 1), filter that adsorbs and inactivates pollen allergen (see Patent Document 2), maintaining pollen allergen in the presence of heat, alkali, acid or protease A method for inactivating pollen allergens (see Patent Documents 3 and 4), a house dust treatment agent containing a koji extract (see Patent Document 5), a ginkgo leaf part, a yellow ginger leaf part, a tea leaf part, Lemon balm leaves and stems, cabbage rose flowers and buds, and allergen inactivator including peanut astringent skin (see Patent Document 6), tea extract, hydroxyapatite, epicatechin, epigallocatechin, epicatechin Anti-allele containing gallate, epigallocatechin gallate, gallic acid and ester compound of gallic acid and alcohol having 1 to 4 carbon atoms Gen composition (see Patent Document 7), organic solvent such as alcohol, polyphenols such as tannic acid, hydroxyapatite, cationic surfactant (particularly a compound having a guanidino group and having a surface active ability or a salt thereof) An antiallergen composition (see Patent Document 8) and the like have been proposed.

In recent years, the mite breeding has been promoted in exchange for the comfort of the indoor environment, and along with the indoor mite breeding, the Dermatophagoides
farinae), Dermatophagoides
allergic diseases in which mites belonging to the genus Lepidoptera, such as Pteronyssinus, are allergens. These mites are considered to contribute to type I allergic diseases such as allergic asthma, rhinitis, conjunctivitis, and atopic dermatitis.

As a countermeasure against allergic diseases in which mites are allergens, it is conceivable to eliminate mites, which are allergens, from the living environment. However, even if the ticks are exterminated, mite allergens cannot be fundamentally eliminated due to the release of powerful allergens from the mite carcasses and mite feces into the living environment. It is difficult to solve. Therefore, there is a need for a technique that fundamentally eliminates mite allergens (such as mites, mite carcasses and feces). Conventionally, as a technique for fundamentally eliminating mite allergens, a technique in which a porous adsorbent impregnated with a rosemary extract is sprayed indoors and sucked with a vacuum cleaner or the like after several hours (see Patent Document 9), etc. Has been proposed.
JP 2002-128659 A JP 2000-5553 A JP 2003-180865 A JP 2004-89673 A JP 2002-128680 A JP 2006-143700 A Japanese Patent Laid-Open No. 06-279273 JP 2000-264837 A Japanese Patent Laid-Open No. 06-256128

An object of the present invention is to find a substance that inactivates pollen allergens and / or mite allergens from a highly safe powder material that can be used in cosmetics, and provides an allergen inactivating agent using the same. It is to provide a cosmetic or allergen inactivating product containing the same.

In order to solve the above problems, the present inventors have intensively studied and found an allergen inactivating effect in silica, fine particle zinc oxide and zeolite having an average particle size of 150 μm or less, and completed the present invention. The allergen as an inactivation target of the allergen inactivating agent is a pollen allergen and / or a mite allergen.

The allergen inactivating agent of the present invention is a cause of allergic symptoms such as hay fever, asthma and atopic dermatitis caused by pollen allergen and / or mite allergen by inactivating pollen allergen and / or mite allergen. Can be prevented, treated or ameliorated.

In the present invention, it has been made possible by using one or more of silica, fine particle zinc oxide and zeolite having an average particle size of 150 μm or less as an allergen inactivating agent and using a moisturizing agent having a high water retaining ability.

“Silica” used in the present invention is silicon dioxide (SiO 2), and is an oxide of silicon, also called silica or silicic acid, and is important as one of the substances forming the crust. Various crystal phases (crystal polymorphs) exist depending on the pressure and temperature conditions. The crystal is a covalent bond crystal and has a structure in which a regular tetrahedral structure centered on a silicon atom is innumerably connected via an oxygen atom. It is applied as a paint inside the bulb so as not to be too bright and is the main component of inorganic glass. And it is used also as a base of powder cosmetics. When the average particle size is larger than 150 μm, the allergen-inactivating effect is obtained to some extent, but when it is formulated, it may feel rough and feel a feeling of use.

The “zinc oxide” used in the present invention is zinc oxide (ZnO) and is also called zinc white. Industrially, it is obtained by heating and vaporizing metallic zinc and burning it with air, or baking zinc sulfate or zinc nitrate. It is important as a white pigment because it has fine particles and is inferior in covering power than lead white but is not toxic and does not turn black with hydrogen sulfide. Other raw materials for pharmaceuticals such as zinc flower ointment and zinc flower starch, and cosmetics.

“Zeolite” used in the present invention is a general term for aluminosilicates having fine pores in crystals. The Japanese name is called fuseki. Used as molecular sieve, ion exchange material, catalyst, adsorbent. At present, zeolites with various properties are artificially synthesized and are industrially important substances.

The present invention can sufficiently exhibit its function even in the presence of water alone or in an environment where water is present. If the powder surface is in a wet state, the effect of capturing allergens is further increased, and the allergen inactivating action is exhibited.

The “humectant” in the present invention is a polyhydric alcohol such as glycerin, 1,3-butylene glycol, 1,2-pentanediol, diglycerin, a natural polymer such as hyaluronic acid, alginic acid, carrageenan, agar, xanthan gum, or carboxyl. Synthetic polymers such as vinyl polymers can be used. Further, the blending amount of the “humectant” in the present invention is not particularly limited as long as it has the effect of the present invention, but in the case of a polyhydric alcohol, it is preferably 0.1 to 20.0% by weight. In the case of a synthetic polymer such as a molecule or a carboxyl vinyl polymer, 0.001 to 0.1% by weight is preferable.

The allergen inactivating agent of the present invention inactivates pollen allergens that cause type I allergic diseases through inactivation of pollen allergens of silica, fine particles of zinc oxide and zeolite having an average particle size of 150 μm or less, such as cedar pollen It is possible to prevent, treat or ameliorate hay fever caused by pollen allergens, and inactivate mite allergens that cause type I allergic diseases through the mite allergen inactivating action of the powder of the present invention. Can prevent, treat or ameliorate type I allergic diseases such as allergic asthma, rhinitis, conjunctivitis, and atopic dermatitis.

The pollen allergen is not particularly limited as long as it is a pollen having an action of causing type I allergic disease, and examples thereof include cedar pollen, cypress pollen, ragweed pollen, camodia pollen, etc. The activator is particularly effective against cedar pollen. The mite allergen is not particularly limited as long as it is a mite having an effect of causing type I allergic disease. For example, mite allergen (Dermatophagoides)
farinae), Dermatophagoides
mite belonging to the genus Lepidoptera, such as Pteronyssinus), feces and dead bodies of these mites.

The blending amount of silica, fine particle zinc oxide and zeolite having an average particle size of 150 μm or less in the cosmetics, house sprays, filters and the like of the present invention is not particularly limited, but is 0.001 to 20 based on the total amount. 0 weight% is preferable and it is more preferable that it is 0.01 to 5.0 weight%. When the blending amount is less than 0.001% by weight, the effect of the present invention may not be sufficiently obtained. On the other hand, when the blending amount exceeds 20.0% by weight, the improvement corresponding to the increase is not recognized. Because there are cases.

In addition to the above essential components, the cosmetic and home spray of the present invention are aqueous components, oily components, plant extracts, animal extracts, powders, excipients, surfactants, oils, alcohols, pH adjusters, preservatives. It is prepared by mixing antioxidants, thickeners, pigments, fragrances and the like as necessary and blending them appropriately. The dosage form of the cosmetic preparation and external preparation for skin of the present invention is not particularly limited, and various dosage forms such as lotion, milky lotion, cream, pack, powder, spray, ointment, dispersion, cleaning agent, and liquid are used. However, it is not limited to the examples given here.

Hereinafter, the present invention will be described with reference to examples. Note that the present invention is not limited to the embodiments.

"Test method and evaluation method"
[Test Example 1] Cedar pollen allergen inactivation test (1) Cedar pollen allergen inactivation reaction 2 mg of each sample is added to a 96-well microplate. 120 μL each of a 2 ng / mL antigen solution in which an antigen (cedar pollen antigen: Cry j 1, manufactured by Seikagaku Corporation) was dissolved in a PBS solution containing 0.1% bovine serum albumin was added to the microplate at room temperature for 2 hours. Shake. After shaking, a mite allergen solution is collected from each well of the microplate into a microtube and centrifuged at 3,000 rpm for 5 minutes. 120 μL of the supernatant after centrifugation was collected, and the cedar pollen allergen concentration (ng / mL) present in the solution was measured by a sandwich ELISA method shown below.

(2) Quantification of cedar pollen allergen concentration (sandwich ELISA method)
100 μL of a 10 μg / mL coating solution (product name: anti-Cry j 1 monoclonal antibody 013, manufactured by Seikagaku Corporation) was added to each well of the ELISA plate and allowed to stand at room temperature for 2 hours. Thereafter, the coating solution was removed, 250 μL of 0.1% bovine serum albumin-containing PBS solution was added, and the mixture was allowed to stand at 4 ° C. overnight. After standing overnight, the PBS solution containing 0.1% bovine serum albumin was removed, 100 μL of the cedar pollen allergen solution shaken for 2 hours was added, and the mixture was shaken at room temperature for 2 hours.

As a standard, Japanese cedar pollen allergen (product name: purified pollen antigen Cry j 1, manufactured by Seikagaku Corporation) was dissolved in PBS solution containing 0.1% bovine serum albumin, 4 ng / mL, 2 ng / mL, 1 ng / mL, Standard solutions for calibration curves of 0.5 ng / mL and 0.25 ng / mL were prepared. 100 μL of standard solution for calibration curve at each concentration was added to the ELISA plate and shaken at room temperature for 2 hours. After shaking, the ELISA plate was washed 3 times with 300 μL of a PBS solution containing 0.05% Tween 20, and then diluted 1000 times to a cedar pollen allergen monoclonal antibody (product name: horseradish peroxidase-labeled anti-Cry j 1 monoclonal antibody 053). 100 μL of Seikagaku Corporation) was added and shaken at room temperature for 2 hours. The ELISA plate was washed three times with 300 μL of PBS solution containing 0.05% Tween 20, and then 100 μL of 0.5 mg / mL substrate solution was added to cause color development at room temperature, followed by reaction for 3 to 5 minutes, and then 2N
The reaction was stopped by adding 100 μL of sulfuric acid, and the absorbance at 490 nm was measured with a microplate reader within 20 minutes.

Using the calibration curve obtained from the absorbance of the standard solution for the calibration curve, the cedar pollen allergen concentration in the sample-added cedar pollen allergen solution and the cedar pollen allergen concentration in the sample-free cedar pollen allergen solution were quantified. The cedar pollen allergen inactivation rate (%) was calculated based on the following formula using the quantitative results.

Using the calibration curve obtained from the absorbance of the standard solution for the calibration curve, the cedar pollen allergen concentration in the sample-added cedar pollen allergen solution and the cedar pollen allergen concentration in the sample-free cedar pollen allergen solution were quantified. The cedar pollen allergen inactivation rate (%) was calculated based on the following formula using the quantitative results.
Cedar pollen allergen inactivation rate (%) = (B−A) / B × 100
In the formula, A represents “the cedar pollen allergen concentration in the sample-added cedar pollen allergen solution (ng / mL)”, and B represents “the cedar pollen allergen concentration in the sample-free cedar pollen allergen solution (ng / mL)”. ".

It was confirmed that the silica, fine particle zinc oxide and zeolite having an average particle size of 150 μm or less have a stronger cedar pollen allergen inactivating effect than hydroxyapatite shown in Patent Document 7 and Patent Document 8.

[Test Example 2] Mite allergen inactivation test 2 mg of each sample is added to a 96-well microplate. 0.05% Tween20 containing PBS solution with antigen (purified mite antigen Der
100 μL of a 400 ng / mL antigen solution in which fII) is dissolved is added to the microplate and shaken at room temperature for 2 hours. After shaking, 70 μL of mite allergen solution is collected from each well of the microplate into a microtube and centrifuged at 3000 rpm for 5 minutes. 50 μL of the supernatant after centrifugation was collected, and the mite allergen concentration (ng / ml) present in the solution was measured by the sandwich ELISA method shown below.

(2) Determination of mite allergen concentration (sandwich ELISA method)
50 μL of 2 μg / mL coating solution (product name: anti-Derf II monoclonal antibody 15E11, manufactured by Seikagaku Corporation) was added to each well of the ELISA plate and allowed to stand at room temperature for 2 hours. Thereafter, the coating solution was removed, washed 3 times with 300 μL of PBS, 200 μL of 1% bovine serum albumin-containing PBS solution was added, and the mixture was allowed to stand at room temperature for 1 hour. After standing for 1 hour, each well was washed 3 times with 300 μL of PBS containing 0.05% Tween 20, 50 μL of standard solution and test solution were added, and the plate was allowed to stand at room temperature for 2 hours. 0.05%
PBS containing Tween 20
Each well was washed three times with 300 μL, 50 μL of 250 pg / mL horseradish peroxidase-labeled anti-Der f II monoclonal antibody 13A4p was added, and the mixture was allowed to stand at room temperature for 2 hours. 0.05%
PBS containing Tween 20
After washing each well 3 times with 300 μL, 4 mg of o-phenylenediamine and 3% hydrogen peroxide are added at a ratio of 20 μL to 10 mL of 0.1 M citric acid and phosphate buffer (pH 5.0). Add 100 μL of substrate solution to each well and let stand for about 15 minutes.
100 μL of sulfuric acid was added per well to stop the enzyme reaction. Within 30 minutes after the addition of sulfuric acid, the absorbance of A490-650 was measured with a plate reader.

Using the calibration curve obtained from the absorbance of the standard curve standard solution, the mite allergen concentration in the sample-added mite allergen solution and the mite allergen concentration in the sample-free mite allergen solution were quantified. The mite allergen inactivation rate (%) was calculated based on the following formula using the quantitative result.
Mite allergen inactivation rate (%) = (B−A) / B × 100
In the formula, A represents “the mite allergen concentration in the sample-added mite allergen solution (ng / mL)”, and B represents “the mite allergen concentration in the sample-free mite allergen solution (ng / mL)”.
The results of the above test are shown in Table 2.

It was confirmed that silica, fine particle zinc oxide and zeolite having an average particle size of 150 μm or less have a stronger mite allergen inactivating action than hydroxyapatite shown in Patent Documents 7 and 8.

[Mite allergen inactivation test]
Sprays were prepared according to the formulations shown in Table 3, and each was sprayed on a non-woven sheet and left for 10 minutes. A 300 mm × 300 mm plastic box was prepared, 50 mm holes were made on both sides, and the dried sheets were installed by spraying the liquids of Examples 1 to 5 and Comparative Examples 1 and 2 on one side. About 10 mL of a 100 ng / mL mite allergen aqueous solution was sprayed into the box, and after standing for 1 hour, the surface on which the sheet was installed was sucked with a vacuum cleaner equipped with a membrane filter in the middle of the hose. The membrane filter was immersed in 5 mL of PBS and stirred well, and then the mite allergen concentration in the solution was measured by a sandwich ELISA method.

The results of evaluation based on the criteria shown in Table 4 are shown in Table 5.

Compared with the sheet (Comparative Example 1) sprayed with the spray not containing the allergen inactivating agent, the allergen amount of the sheet containing the allergen inactivating agent (Comparative Example 2, Examples 1 to 6) decreased. In addition, even when compared with hydroxyapatite (Comparative Example 2), the powders of Examples (Examples 1 to 6) of the present invention showed a significant decrease in allergen. Furthermore, compared with Example 3 which does not contain a humectant, Example 1, Example 2 and Examples 4 to 6 containing a humectant showed a significant decrease in allergen. This result indicates that when the powder of the present invention and a humectant are used in combination, the allergen inactivating effect is synergistically enhanced.

The formulation example of this invention is given to the following.

<Formulation example 1> lotion (component name) (mass%)
Silica (Silicia 350) 0.5
Fine zinc oxide 0.1
Glycerin 5.0
Polyoxyethylene sorbitan monolaurate (20E.0) 1.5
Sodium hyaluronate 0.1
Agar 0.001
Ethanol 8.0
Triethyl citrate 2.0
Preservative / Antioxidant
Purified water balance

<Formulation example 2> Emulsion (component name) (mass%)
Silica (Erogil 200) 0.0001
Zeolite 0.01
Squalane 8.0
Vaseline 2.0
Beeslow 0.5
Sorbitan sesquioleate 0.8
Polyoxyethylene oleyl ether (20E.0) 1.2
Carboxyvinyl polymer 0.2
Carrageenan 0.01
Glycerin 1.5
Potassium hydroxide 0.1
Ethanol 7.0
Glycerin 2.0
Perfume Appropriate amount of preservative / antioxidant Appropriate amount of purified water

<Prescription Example 3> Ointment (component name) (mass%)
Silica (Silicia 350) 1.0
Fine zinc oxide 0.5
Tocopherol acetate 0.5
Octyl paradimethylaminobenzoate 4.0
Butylmethoxybenzoylmethane 4.0
Stearyl alcohol 18.0
Owl 20.0
Glycerin monostearate 0.3
Vaseline 33.0
Perfume Appropriate amount of preservative / antioxidant Appropriate amount of purified water

<Formulation example 4> Gel <Ingredient name> (wt%)
Silica (Erogil 200) 5.0
Zeolite 5.0
Ethanol 10.0
Pectin 1.0
Xanthan gum 0.2
Alginic acid 0.01
1,3-BG 5.0
Diglycerin 0.5
KOH proper amount Sodium chloride 0.02
Purified water balance

<Prescription Example 5> Powdery Foundation
<Ingredient name> (wt%)
Silica (Spar microbead silica C-30 10.0
Fine zinc oxide 5.0
Talc 5.0
Mica 35.0
Kaolin 5.0
Titanium oxide 10.0
Mica titanium 3.0
Zinc stearate 1.0
Nylon powder 10.0
Squalane 6.0
Lanolin acetate 1.0
Sorbitan monooleate 1.0
1,3-butylene glycol 2.0
Preservatives and antioxidants Appropriate amount Color pigment Appropriate amount

<Prescription Example 6> Spray silica for houses (Silycia 350) 2.0
1,3-butylene glycol 5.0
1,2-pentanediol 2.0
Polyoxyethylene sorbitan monolaurate (20E.0) 1.5
Ethanol 15.0
Preservative / Antioxidant
Purified water balance

The present invention can prevent itching and inflammation caused by allergens, directly capture allergens that cause allergies, and prevent, treat or ameliorate the causes of allergic symptoms such as hay fever, asthma and atopic dermatitis. Therefore, it can be widely applied to products for inactivating allergens such as indoor mist / spray, fiber, non-woven fabric, paper, and filter.

Claims (6)

An allergen deactivator comprising silica and / or zinc oxide and / or zeolite and a humectant as active ingredients. 2. The allergen inactivating agent according to claim 1, wherein the average particle size of silica is 150 [mu] m or less. 2. The allergen according to claim 1, wherein the humectant is glycerin, 1,3-butylene glycol, 1,2-pentanediol, diglycerin, hyaluronic acid, alginic acid, carrageenan, agar, xanthan gum, carboxyl vinyl polymer. Inactivator. 2. The allergen inactivating agent according to claim 1, wherein the allergen is a pollen allergen and / or a mite allergen. A cosmetic comprising the allergen inactivating agent according to claim 1. An allergen inactivating spray comprising the allergen inactivating agent according to claim 1.