JP2008156314A - Atp production promotor - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a new ATP (adenosine triphosphate) production promoter having excellent ATP production promoting effect and stably keeping the effect. <P>SOLUTION: The ATP production promotor comprises colloid of at least one metal selected from the platinum group as an active ingredient. Preferably, the ATP production promotor comprises platinum as the metal. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to a novel ATP production promoter that can be blended in an external preparation for skin such as cosmetics.

  ATP is involved in many energy metabolisms as a biological energy transmitter, and plays an important role in the acquisition and use of energy. Organelle mitochondria play a major role in oxidative phosphorylation, which synthesizes ATP using the energy of substance oxidation. If the ability to synthesize ATP can be increased, it is considered that the energy source can be increased and the vital activity ability of the living body can be increased.

  Conventionally, a muscle cell ATP production promoter containing yeast extract as an active ingredient has been provided for the purpose of improving skin sagging and firmness (Patent Document 1). In addition, for the purpose of activating epidermal cells by promoting ATP production of epidermal cells, an ATP production promoter containing glycogen as an active ingredient has been proposed (Patent Document 2). Thus, various searches for agents that promote ATP production have been conducted and used. However, many of the conventionally proposed ATP production promoters are easily affected by the external environment such as the liquidity and temperature change of the medium, and there are cases where chemical stability may be a problem, and an ATP production effect is exhibited. It is considered that the process itself often changes in the process, and it is desired to provide a new ATP production promoter having improved such points.

  On the other hand, platinum group metal colloids have been confirmed to have an antioxidant effect, a whitening effect, an atopy improving effect, etc., and are conventionally blended in pharmaceuticals, cosmetics, and beverages, but still have no relation to ATP production. It was not confirmed.

JP 2001-10947 A JP 2003-321373 A

  This invention makes it a subject to provide the novel ATP production promoter which has the outstanding ATP production promotion effect and can maintain the effect stably.

  In order to solve the above-mentioned problems, the ATP production promoter of the present invention contains at least one colloid of a metal species selected from the platinum group as an active ingredient. As a preferred embodiment of the present invention, an ATP production promoter containing platinum colloid as an active ingredient is provided. From another point of view, according to the present invention, there is provided a method for promoting ATP production by supplying a colloid of at least one metal selected from the platinum group (except for a human therapeutic method).

  ADVANTAGE OF THE INVENTION According to this invention, while having the outstanding ATP promotion effect, the novel ATP production promoter which can maintain the effect stably can be provided.

Hereinafter, the present invention will be described in detail. In the present specification, “to” is used to mean that the numerical values described before and after it are included as a lower limit value and an upper limit value.
The ATP production promoter of the present invention comprises at least one metal colloid selected from the platinum group as an active ingredient. Platinum group metal colloids are excellent in chemical stability and are not easily affected by the external environment. Furthermore, since the mechanism of the ATP production promoting effect is mainly a catalytic reaction of a platinum group metal colloid, the essence of the agent does not change during the process of promoting ATP production (theoretically, the effect is permanent). ), An excellent ATP production promoting effect can be expected for a long time. Since the promotion of ATP production leads to an improvement in the metabolic function of cells, the ATP production promoter of the present invention is useful for application to cosmetics, health foods and beverages for the purpose of anti-fatigue and slimming.

  The platinum group metal is a generic name for transition metals composed of ruthenium, osmium, rhodium, iridium, palladium, and platinum. Of these, rhodium and platinum are preferable, and platinum is most preferable. The platinum group metal colloid means a state in which these platinum group metal particles are dispersed in a liquid phase. It may be a kind of colloid selected from platinum group metals, or a colloid of two or more kinds of metals. Among these, a colloid of a single metal species is preferable, and a platinum colloid or a rhodium colloid is preferable.

  The particle size of the platinum group metal particles is not particularly limited, but is preferably a nano unit. Specifically, the particle size is preferably 1 to 5 nm, more preferably 1 to 3 nm, and even more preferably 1.5 to 2.5 nm. The particle size distribution of the particles is preferably narrow. Specifically, the particle size of 90% by number or more of colloidal particles preferably falls within the range of 0.1 to 10 nm, and falls within the range of 1 to 3 nm. Is more preferable. The average particle size of the colloidal particles can be determined by observing with a transmission electron microscope.

  The main component of the liquid phase in which the platinum group metal particles are dispersed is preferably water. An organic solvent may be contained as long as the colloidal state is not destabilized. Examples of the organic solvent include alcohols such as methyl alcohol and ethyl alcohol; polyhydric alcohols such as glycerin, diglycerin, 1,3-butylene glycol, and dipropylene glycol.

  Various methods for preparing metal colloids or metal particles are known (for example, JP-B-57-43125, JP-A-59-120249, JP-A-9-225317, JP-A-10-176207). JP, 2001-79382, JP, 2001-122723, etc.). For example, as a method for producing (metal colloid or metal particles), a chemical method called a precipitation method or a metal salt reduction reaction method, or a physical method called a combustion method is known. For the ATP production promoter of the present invention, a metal colloid prepared by any method may be used, but from the viewpoint of ease of production and quality, a metal colloid prepared by a metal salt reduction reaction method is used. Alternatively, it is preferable to prepare and use the metal particles obtained by the above method as a colloid.

  In the metal salt reduction reaction method, for example, an aqueous solution or organic solvent solution of a water-soluble or organic solvent-soluble metal salt or metal complex is prepared, and after adding a water-soluble polymer to this solution, the pH is adjusted as necessary. The metal particles can be obtained by reduction by heating under reflux in an inert atmosphere. The type of the water-soluble or organic solvent-soluble salt of the metal is not particularly limited. For example, acetate, chloride, sulfate, nitrate, sulfonate, or phosphate can be used. It may be used.

  The type of water-soluble polymer used in the metal salt reduction reaction method is not particularly limited. For example, polyvinylpyrrolidone, polyvinyl alcohol, polyacrylate, cyclodextrin, amylopectin, or methylcellulose can be used, and two of these can be used. A combination of the above may also be used. Preferably, polyvinylpyrrolidone and polyacrylate can be used, and more preferably, polyacrylate can be used. Further, various surfactants such as anionic, cationic, amphoteric or nonionic surfactants can be used instead of or together with the water-soluble polymer. When the reduction is performed using alcohol or glycol, ethyl alcohol, n-propyl alcohol, n-butyl alcohol, n-amyl alcohol, ethylene glycol, or the like is used. These water-soluble polymers and surfactants are coordinated to platinum group metals after the formation of metal particles in the liquid phase, improving the solvophilicity of the metals, and in colloidal state as protective colloid agents. Contributes to stabilization.

  The ATP production promoter of the present invention is preferably blended in a skin external preparation such as an external medicine or cosmetic. When preparing the skin external preparation, a conventionally known external pharmaceutical additive or skin cosmetic additive may be blended together with the ATP production promoter of the present invention. The preferred range of the amount of the ATP production promoter of the present invention in the external preparation for skin varies depending on the dosage form of the external preparation, the purpose of use, etc., but in general, the concentration of the platinum group metal in the final composition, Preferably they are 0.01 nmol / L-50 micromol / L, More preferably, they are 1 nmol / L-1 micromol / L. Within this range, the colloid can be blended stably, and an excellent ATP production promoting effect can be exhibited.

  Additives added to the skin external preparation as necessary include water (purified water, hot spring water, deep water, etc.), alcohols, oils, which are commonly used in skin cosmetics and pharmaceutical preparations for external use. Surfactant, metal soap, gelling agent, powder, alcohol, water-soluble polymer, film forming agent, resin, UV protection agent, inclusion compound, antibacterial agent, fragrance, deodorant, salt, pH adjuster , Fresheners, animal / microbe-derived extracts, plant extracts, blood circulation promoters, astringents, antiseborrheic agents, whitening agents, anti-inflammatory agents, active oxygen scavengers, cell activators, moisturizers, chelating agents, keratins Examples include solubilizers, enzymes, hormones, and vitamins. The external preparation for skin can be prepared according to a conventional method, and the blending amount of the additive can also be determined according to a conventional method as long as the effects of the present invention are not impaired.

  The form of the external preparation for skin is not limited, and forms belonging to skin cosmetics such as emulsions, creams, skin lotions, cosmetic liquids, packs, face wash, makeup cosmetics, shampoos, hair treatments, hair styling agents, It may be in any form relating to hair cosmetics such as hair nourishing agents and hair restorers; and forms of external pharmaceuticals such as dispersions, ointments, aerosols, patches, poultices, liniments and the like.

  Moreover, the ATP production promoter of this invention can also be mix | blended with foodstuffs (a drink is included). The food is not particularly limited, and examples include salmon, chewing gum, health food, and beverages.

EXAMPLES Hereinafter, although an Example demonstrates this invention further more concretely, the scope of the present invention is not limited to the following Example.
[Example 1: Preparation of ATP production promoter]
Poly (sodium polyacrylate) (manufactured by Aldrich, 0.31 g) was placed in a 100 mL two-necked eggplant type flask connected with an Allen cooler and a three-way cock, and dissolved in 23 mL of distilled water. After stirring this solution for 10 minutes, a 1.66 × 10 ⁻² mol / L solution (2 mL) in which chloroplatinic acid (H ₂ PtCl ₆ .6H ₂ O, manufactured by Wako Pure Chemical Industries, Ltd.) was dissolved in distilled water was added. In addition, the mixture was further stirred for 30 minutes. The reaction system was purged with nitrogen, 25 mL of special grade ethyl alcohol was added, and the mixture was refluxed at 100 ° C. for 2 hours while maintaining a nitrogen atmosphere. The UV-visible light absorption spectrum of the reaction solution was measured to confirm the disappearance of the platinum ion peak and the saturation of the peak due to the scattering characteristic of the metal solid, and the reduction reaction was completed. The organic solvent was distilled off under reduced pressure to prepare an ATP production promoter as platinum colloid (average particle size 2.4 ± 0.7 nm). The platinum concentration of the obtained ATP production promoter was 1 mmol / L.

[Example 2: Evaluation of ability to promote ATP production]
Human neonatal fibroblast NB1RGB was cultured in DMEM with 1% FBS. At that time, the prepared ATP production promoter (namely, 1 mmol / l platinum colloid) was adjusted so that the concentration per unit volume of the medium was 0, 0.001 vol / vol%, 0.01 vol / vol%. It has been added to the medium. After 5 days of culture, the cells were collected and treated with platinum colloids of various concentrations. The cells were taken to a fixed cell count microplate, and 100 μL of ATP measurement reagent (manufactured by Toyo B-Net Co., Ltd.) was added and stirred for 10 minutes. After the reaction, luminescence was measured using a luminometer, and the amount of ATP produced by the cells was obtained from a calibration curve using a separately prepared ATP solution. The ATP production promotion rate was calculated from the ratio (ATP production amount of cells added with platinum colloid / ATP production amount of cells not added with platinum colloid × 100) compared with the ATP production amount of cells not added with platinum colloid. The results are shown in Table 1 below.

[Example 3: lotion]
A lotion containing the ATP production promoter of the present invention was prepared by the following method.
A. The following components (3) to (5), (12) and (13) are mixed and dissolved.
B. The following components (1), (2), (6) to (11) and (14) are mixed and dissolved.
C. A and B were mixed and uniformed to obtain a skin lotion.

(Ingredient) (%)
(1) Glycerin 5.0
(2) 1,3-butylene glycol 6.5
(3) Polyoxyethylene (20E.O.) sorbitan 1.2
Monolaurate (4) Ethyl alcohol 12.0
(5) Ascorbic acid
Palmitic acid ester * 1 0.1
(6) Sodium ascorbate phosphate * 2 0.5
(7) Ascorbic acid glucoside * 3 2.0
(8) Lactic acid 0.05
(9) Sodium lactate 0.1
(10) ATP production promoter (1 mmol / L platinum colloid) 0.05
(11) Collagen 1.0
(12) Preservative Appropriate amount (13) Fragrance Appropriate amount (14) Purified water Remaining amount * 1 Wako Pure Chemicals * 2 Sigma Co. * 3 Hayashibara Biochemical Research Institute

  The skin lotion prepared in Example 3 is stable without discoloration, odor and precipitation, has a fresh moisturizing feeling when applied to the skin, and has the effect of making the skin healthy by applying continuously. It was.

[Example 4: Preparation of emulsion]
An emulsion containing the ATP production promoter of the present invention was prepared by the following method.
(Manufacturing method)
A. The following components (12) to (14) are heated and mixed and maintained at 70 ° C.
B. The following components (1) to (9) are heated and mixed and maintained at 70 ° C.
C. A is added to B, mixed and uniformly emulsified.
D. After cooling C, the following components (10) and (11) are added and mixed uniformly.
E. The following component (15) was added to D, sufficiently stirred, and the following components (16) to (18) were further added and mixed uniformly to obtain an emulsion.

(Ingredient) (%)
(1) Polyoxyethylene (10E.O.) sorbitan 1.0
Monostearate (2) Polyoxyethylene (60EO) Sorbit 0.5
Tetraoleate (3) Glyceryl monostearate 1.0
(4) Stearic acid 0.5
(5) Behenyl alcohol 0.5
(6) Squalane 8.0
(7) Retinol palmitate * 1 0.0002
(8) Ethyl alcohol 5.0
(9) Daylily extract * 2 0.01
(10) Dipotassium glycyrrhizinate * 3 0.1
(11) ATP production promoter (1 μmol / L platinum colloid) 0.2
(12) Purified water remaining amount (13) Preservative 0.1
(14) Carboxyvinyl polymer 0.2
(15) Sodium hydroxide 0.1
(16) Hyaluronic acid 0.1
(17) Zinc oxide 3.0
(18) Fragrance Appropriate amount * 1 Made by Nippon Roche * 2 Made by Maruzen Pharmaceutical * 3 Made by Maruzen Pharmaceutical

  The emulsion prepared in Example 4 is stable without discoloration and odor, has a high smooth emollient effect when applied to the skin, and has an excellent effect of suppressing or improving wrinkle formation by continuous application. It was.

[Example 5: Cream]
A cream containing the ATP production promoter of the present invention was prepared by the following method.
(Manufacturing method)
A. The following components (1) to (10) are heated and mixed and maintained at 70 ° C.
B. The following components (11) to (16) and (19) to (21) are heated and mixed and maintained at 70 ° C.
C. A is added to B, mixed and uniformly emulsified.
D. After cooling C, the following components (17) and (18) were added and mixed uniformly to obtain a cream.

(Ingredient) (%)
(1) Cetostearyl alcohol 3.0
(2) Glycerin fatty acid ester 2.0
(3) Polyoxyethylene monooleate (20) sorbitan 1.0
(4) Sorbitan monostearate 1.0
(5) N-stearoyl-N-methyltaurine sodium 0.5
(6) Vaseline 5.0
(7) Methylpolysiloxane (100 mm ² / s) 3.0
(8) Glyceryl tri-2-ethylhexanoate 20.0
(9) dl-α-tocopherol * 1 1.0
(10) Ellagic acid * 2 0.05
(11) Lactic acid (50% aqueous solution) 1.0
(12) Dipropylene glycol 10.0
(13) Arbutin * 3 3.0
(14) Sodium citrate 0.5
(15) ATP production promoter (1 μmol / L platinum colloid) 0.5
(16) L-ascorbic acid
Phosphate ester magnesium salt * 4 0.1
(17) Titanium oxide 0.1
(18) Perfume appropriate amount (19) Disodium edetate 0.03
(20) Preservative Appropriate amount (21) Remaining amount of purified water * 1 Eisai * 2 Sigma * 3 Wako Pure Chemical * 4 Wako Pure Chemical

  The cream prepared in Example 5 is stable without discoloration, odor, separation, etc., and has a smooth moisturizing feeling when applied to the skin, and has the effect of suppressing or improving blots and dullness when applied continuously. There was something.

[Example 6: Beverage]
A beverage blended with the ATP production promoter of the present invention was prepared by the following method.
(Manufacturing method)
A stable beverage was obtained by mixing and dissolving the following components (1) to (4).
(Ingredient) (%)
(1) ATP production promoter (1 μmol / L platinum colloid) 0.1
(2) Sodium ascorbate 0.1
(3) Citric acid 0.2
(4) Water remaining

  The ATP production promoter of the present invention has an effect of improving the metabolic function of cells and can be blended in cosmetics, health foods and beverages for the purpose of anti-fatigue and slimming.

Claims (2)

An ATP production promoter comprising, as an active ingredient, a colloid of at least one metal selected from the platinum group. The ATP production promoter according to claim 1, wherein the at least one metal is platinum.