JP2007010584A - Method and device for evaluating amount of urea in object, method and device for evaluating amounts of urea and moisture in object, program, and computer-readable recording medium - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a method of evaluating amounts of urea and moisture in an object capable of evaluating the amounts of the urea and the moisture in the object in vivo. <P>SOLUTION: This method of evaluating the amounts of the urea and the moisture in the object includes a step for irradiating the object with a near infrared ray containing at least one wavelength within 1850 nm or more and less than 1950 nm of range, and at least one wavelength within 1950 nm or more and less than 2000 nm of range (S2), a step for obtaining a spectral data of the near infrared ray reflected from the object (S4), and a step for evaluating the amount of the urea and the amount of the moisture in the object, using the spectral data of the near infrared ray. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a method for evaluating the amount of urea in a subject, a method for evaluating the amount of urea and moisture in a subject, a program, a computer-readable recording medium, a device for evaluating the amount of urea in a subject, and urea and The present invention relates to an apparatus for evaluating the amount of moisture.

  In recent years, in the cosmetics industry, it has been desired to immediately provide cosmetics suitable for the user's skin, nails and the like from many cosmetics at stores and the like.

  For this purpose, before applying cosmetics to the user's skin, nails, etc., the amount of moisture and / or sebum in the user's skin, the amount of moisture in the user's nails, Appropriate cosmetics for the user's skin, nails, etc. based on the quantitative measurement and the amount of moisture and / or sebum in the measured user's skin and the measured amount of moisture in the user's nails It is possible to provide.

  Thus, before applying cosmetics to the user's skin, nails, etc., as a method for immediately and quantitatively measuring the amount of water and / or sebum in the user's skin by a simple method, for example, The method of measuring the quantity of the water | moisture content and / or sebum in a user's skin using near-infrared spectroscopy as disclosed by patent document 1 and nonpatent literature 1 is mentioned.

  Moreover, as a method of measuring the amount of water in the user's nail immediately and quantitatively by a simple method, for example, using near infrared spectroscopy as disclosed in Patent Document 2, A method for measuring the amount of water in the nail.

  On the other hand, after applying cosmetics to the user's skin, nails, etc., the suitability of the cosmetics to the user's skin, nails, etc. is immediately and quantitatively evaluated by a simple method, and applied to the user's skin, nails, etc. It is also conceivable to provide cosmetics to the user based on the evaluation of the suitability of the cosmetics. In this case as well, after applying cosmetics to the user's skin, nails, etc., a method for immediately and quantitatively evaluating the suitability of the cosmetics to the user's skin, nails, etc. by a simple method is required.

  For example, for cosmetics containing urea, urea contained in cosmetics may penetrate into human skin and have a therapeutic effect for treating rough skin and roughness of human skin and a moisturizing effect for maintaining moisture in human skin. Are known. More specifically, urea has a stratum corneum moisture retention action and a protein denaturation action on the skin, depending on the urea concentration. Therefore, when cosmetics containing urea are applied to human skin, the permeability of urea to the skin is simplified as a guideline for evaluating the therapeutic and moisturizing effects of urea that has penetrated the skin on which cosmetics containing urea are applied. It is desirable to evaluate immediately and quantitatively by a simple method.

  Here, as the quantitative analysis method for urea, the urea in the active substance and product such as high performance liquid chromatography (HPLC) using internal standard method, Kjeldahl nitrogen determination method, etc. are measured in vitro. A method is mentioned. However, these methods are methods for measuring urea in the active ingredient and product in vitro, and using these methods to evaluate the amount of urea that has penetrated human skin in vivo. I can't.

Moreover, the method of using an infrared absorption spectrum is also mentioned as a qualitative analysis method regarding urea. However, since human skin contains a large amount of moisture, in the infrared absorption spectrum of human skin to which cosmetics containing urea are applied, the vicinity of 3200-3600 cm ⁻¹ due to moisture contained in the skin is present. A broad peak is detected. For this reason, the peak due to urea that has penetrated into the skin is covered with a broad peak in the vicinity of 3200-3600 cm ⁻¹ due to moisture contained in the skin. As a result, it is not possible to evaluate in vivo the amount of urea that has penetrated human skin using infrared absorption spectra.

Therefore, a method for evaluating the amount of urea penetrating into human skin in vivo is desired.
JP 2002-90298 A JP 2003-344278 A Published technique of technique number 2004-506411 ("Method and apparatus for measuring the amount of moisture and sebum in the skin, program, and computer-readable program medium")

  An object of the present invention is to provide a method for evaluating the amount of urea in a subject and an apparatus for evaluating the amount of urea in a subject, which can evaluate the amount of urea in a subject in vivo.

  In addition, the present invention provides a method for evaluating the amount of urea and moisture in a subject and a device for evaluating the amount of urea and moisture in a subject, which can evaluate the amount of urea and moisture in a subject in vivo. With the goal.

  Furthermore, the present invention provides a program that allows a computer to execute an in vivo evaluation of the amount of urea or urea and water in a subject, and a computer-readable recording medium on which the program is recorded. The purpose is to provide.

The invention according to claim 1, in the method for evaluating the amount of urea in the object, irradiating the object with near infrared light including at least one wavelength in the range of 1950 nm to 2000 nm, the near light reflected from the object Obtaining infrared spectral data, and evaluating the amount of urea in the object using the infrared spectral data.

  According to the first aspect of the present invention, it is possible to provide a method for evaluating the amount of urea in a subject, in which the amount of urea in the subject can be evaluated in vivo.

  The invention according to claim 2 is the method for evaluating the amount of urea in the subject according to claim 1, characterized in that the subject is the skin of a human eyelid.

  According to the second aspect of the present invention, it is possible to provide a method for evaluating the amount of urea in a subject, in which the amount of urea in the skin of a human eyelid can be evaluated in vivo.

  According to a third aspect of the present invention, there is provided a method for evaluating the amount of urea and moisture in a subject, wherein near infrared rays including at least one wavelength in a range from 1850 nm to 1950 nm and at least one wavelength in a range from 1950 nm to 2000 nm are used. Irradiating the object, obtaining the near-infrared spectrum data reflected from the object, and using the near-infrared spectrum data to evaluate the amount of urea and moisture in the object. It is characterized by.

  According to the third aspect of the present invention, it is possible to provide a method for evaluating the amount of urea and moisture in the subject, which can evaluate the amount of urea and moisture in the subject in vivo.

  The invention according to claim 4 is the method for evaluating the amount of urea and water in the subject according to claim 3, wherein the subject is skin of human eyelids.

  According to the invention described in claim 4, it is possible to provide a method for evaluating the amount of urea and water in a subject, which can evaluate in vivo the amount of urea and water in the skin of a human eyelid.

  The invention according to claim 5, in the program, irradiating an object with near infrared light including at least one wavelength in a range of 1950 nm to 2000 nm, obtaining spectral data of the near infrared light reflected from the object, And using the near-infrared spectrum data to cause the computer to execute a step of evaluating the amount of urea in the object.

  According to the fifth aspect of the present invention, it is possible to provide a program capable of causing a computer to evaluate the amount of urea in a subject in vivo.

  According to a sixth aspect of the present invention, in the program, the step of irradiating the object with near infrared light including at least one wavelength in the range of 1850 nm to less than 1950 nm and at least one wavelength in the range of 1950 nm to 2000 nm, reflected from the object Obtaining the near-infrared spectrum data, and using the near-infrared spectrum data to evaluate the amount of urea and the amount of water in the object.

  According to the sixth aspect of the present invention, it is possible to provide a program that allows a computer to execute in vivo evaluation of the amount of urea and water in a subject.

  The invention according to claim 7 is characterized in that the program according to claim 5 or 6 is recorded in a computer-readable recording medium.

  According to invention of Claim 7, the computer-readable recording medium with which the program which can make a computer perform the evaluation of the quantity of urea in the object or the quantity of urea and water in vivo is recorded Can be provided.

The invention according to claim 8 is an apparatus for evaluating the amount of urea in a target, wherein the target is irradiated with near infrared rays including at least one wavelength in the range of 1950 nm to 2000 nm, and the near reflected from the target Means for obtaining infrared spectrum data, and means for evaluating the amount of urea in the object using the near-infrared spectrum data.

  According to the eighth aspect of the present invention, it is possible to provide an apparatus for evaluating the amount of urea in a subject, which can evaluate the amount of urea in the subject in vivo.

  The invention according to claim 9 is an apparatus for evaluating the amount of urea and moisture in a subject, and includes a near infrared ray including at least one wavelength in the range of 1850 nm to 1950 nm and at least one wavelength in the range of 1950 nm to 2000 nm. Means for irradiating the object, means for obtaining the near-infrared spectrum data reflected from the object, and means for evaluating the amount of urea and water in the object using the near-infrared spectrum data It is characterized by.

  According to the ninth aspect of the present invention, it is possible to provide an apparatus for evaluating the amount of urea and moisture in the subject, which can evaluate the amount of urea and moisture in the subject in vivo.

  According to the present invention, it is possible to provide a method for evaluating the amount of urea in a subject and an apparatus for evaluating the amount of urea in a subject, which can evaluate the amount of urea in the subject in vivo.

  In addition, according to the present invention, there are provided a method for evaluating the amount of urea and moisture in a subject and an apparatus for evaluating the amount of urea and moisture in a subject, which can evaluate the amount of urea and moisture in a subject in vivo. can do.

  Furthermore, according to the present invention, a program capable of causing a computer to evaluate the amount of urea or urea and water in a subject in vivo, and a computer-readable record in which the program is recorded A medium can be provided.

  Next, embodiments of the present invention will be described with reference to the drawings.

  The method for evaluating the amount of urea in a target according to the first embodiment of the present invention includes irradiating a target with near infrared light including at least one wavelength in the range of 1950 nm to 2000 nm, and the near infrared light reflected from the target. Obtaining spectral data, and using infrared spectral data to assess the amount of urea in the subject.

  According to the method for evaluating the amount of urea in a subject according to the first embodiment of the present invention, the amount of urea in the subject can be evaluated in vivo.

  A method for evaluating the amount of urea and moisture in a subject according to the second embodiment of the present invention includes a near infrared ray including at least one wavelength in the range of 1850 nm to 1950 nm and at least one wavelength in the range of 1950 nm to 2000 nm. Irradiating the object, obtaining near-infrared spectral data reflected from the object, and using the near-infrared spectral data to evaluate the amount of urea and the amount of moisture in the object.

  According to the method for evaluating the amount of urea and moisture in the subject according to the second embodiment of the present invention, the amount of urea and moisture in the subject can be evaluated in vivo. In particular, both the amount of urea and moisture in the subject can be assessed in vivo at once.

  An apparatus for evaluating the amount of urea in a target according to the third embodiment of the present invention is a means for irradiating a target with near-infrared rays including at least one wavelength in the range of 1950 nm to 2000 nm. Means for obtaining spectral data, as well as means for evaluating the amount of urea in the object using near infrared spectral data.

  According to the apparatus for evaluating the amount of urea in the subject according to the third embodiment of the present invention, the amount of urea in the subject can be evaluated in vivo.

  An apparatus for evaluating the amount of urea and moisture in a subject according to the fourth embodiment of the present invention includes a near infrared ray including at least one wavelength in the range of 1850 nm to 1950 nm and at least one wavelength in the range of 1950 nm to 2000 nm. Means for irradiating the object, means for obtaining near-infrared spectrum data reflected from the object, and means for evaluating the amount of urea and water in the object using the near-infrared spectrum data.

  According to the apparatus for evaluating the amount of urea and moisture in the subject according to the fourth embodiment of the present invention, the amount of urea and moisture in the subject can be evaluated in vivo. In particular, both the amount of urea and moisture in the subject can be assessed in vivo at once.

  The program according to the fifth embodiment of the present invention includes a step of irradiating an object with near infrared rays including at least one wavelength in a range of 1950 nm to 2000 nm, obtaining near infrared spectrum data reflected from the object, and Using the infrared spectral data, the computer is caused to perform a step of evaluating the amount of urea in the subject.

  According to the program according to the fifth embodiment of the present invention, it is possible to cause a computer to evaluate the amount of urea in a subject in vivo.

  The program according to the sixth embodiment of the present invention includes a step of irradiating the object with near infrared light including at least one wavelength in the range of 1850 nm to less than 1950 nm and at least one wavelength in the range of 1950 nm to 2000 nm, reflected from the object. Further, the computer is caused to execute a step of obtaining near-infrared spectrum data and a step of evaluating the amount of urea and the amount of moisture in the object using the near-infrared spectrum data.

  According to the sixth embodiment of the present invention, it is possible to cause a computer to evaluate the amount of urea and water in a subject in vivo. In particular, it is possible to have the computer perform an in vivo assessment of both the amount of urea and water in the subject at once.

  The computer-readable recording medium according to the seventh embodiment of the present invention records the program according to the fifth or sixth embodiment of the present invention.

  According to the computer-readable recording medium according to the seventh embodiment of the present invention, it is possible to cause the computer to evaluate in vivo the amount of urea or the amount of urea and water in the subject.

Here, at least one wavelength in the range from 1950 nm to 2000 nm is a wavelength absorbed by the urea molecule. More specifically, at least one wavelength in the range from 1950 nm to 2000 nm corresponds to the combined sound of the NH bond stretching vibration and the NH bending vibration in the urea molecule (NH ₂ CONH ₂ ).

In addition, at least one wavelength in the range from 1850 nm to less than 1950 nm is a wavelength that is absorbed by water molecules. More specifically, at least one wavelength in the range from 1850 nm to less than 1950 nm corresponds to the combined sound of OH bond stretching vibration and OH bond bending vibration in water molecules (H ₂ O).

  The range of the near-infrared wavelength including at least one wavelength in the range from 1950 nm to 2000 nm (and at least one wavelength in the range from 1850 nm to less than 1950 nm) is not particularly limited. For example, the wavelength is from 1800 nm to 2050 nm It may be a range.

  The object is the near-infrared intensity data having at least one wavelength in the range from 1950 nm to 2000 nm in the spectral data (and the near-infrared intensity data having at least one wavelength in the range from 1850 nm to less than 1950 nm). If it can obtain, it will not specifically limit. The intensity of near-infrared light having at least one wavelength in the range of 1950 nm to 2000 nm (and the intensity of near-infrared light having at least one wavelength in the range of 1850 nm to less than 1950 nm) is absorbed by urea (and moisture). Depending on, it has a value greater than or equal to 0 (can also include a value of 0).

  However, in the method for assessing the amount of urea (and moisture) in a subject, the subject is preferably human eyelid skin. In this case, the amount of urea (and moisture) in the human eyelid skin can be evaluated in vivo. Human eyelid skin has a thick stratum corneum with a thickness of about 300 μm to 1 mm. In the stratum corneum of human skin, the amount of water is small compared to the epidermis of human skin and the like (usually the water content is in the range of 20-30% to 70-80%). For this reason, in the infrared spectrum reflected from the object, the near-infrared absorption peak having at least one wavelength in the range from 1950 nm to 2000 nm has the near-infrared wavelength having at least one wavelength in the range from 1950 nm to 2000 nm. Separated from the absorption peak of. That is, the intensity of near-infrared light having at least one wavelength in the range of 1950 nm to 2000 nm (and the intensity of near-infrared light having at least one wavelength in the range of 1850 nm to less than 1950 nm) can be easily obtained.

  Urea in the subject may be present on the surface of the subject or may be present inside the surface of the subject. Further, urea in the subject may be originally included in the subject, and a material containing urea may be applied to the subject. The use of the material containing urea is not particularly limited, and may be, for example, a cosmetic product or a pharmaceutical product. Moreover, the form of the material containing urea is not particularly limited, and may be, for example, a liquid such as an aqueous solution or a cream. The urea content (%) in the material containing urea is not particularly limited as long as the intensity of near infrared rays having at least one wavelength in the range of 1950 nm to 2000 nm can be obtained.

  For example, a cosmetic or pharmaceutical product containing urea may be applied to the skin of a human eyelid. When a cosmetic or pharmaceutical product containing urea is applied to the skin of a human eyelid and the amount of urea that has penetrated into the skin of the human eyelid is evaluated, the urea-containing cosmetic or pharmaceutical product is preferably a cream containing urea. It is. Since human skin contains sebum, urea contained in the cream can be efficiently penetrated into the skin by using a cream containing oil. In order to sufficiently penetrate urea into the skin of a human eyelid, after applying a cosmetic or pharmaceutical product containing urea to the skin of a human eyelid, the surface of the skin to which the cosmetic or pharmaceutical product containing urea has been applied is applied. Alternatively, it may be covered with a resin film. Thereafter, the resin film is removed from the surface of the skin, and if necessary, cosmetics or pharmaceuticals remaining on the surface of the skin are sufficiently wiped with paper or the like. The amount of urea that has permeated the surface of the skin is evaluated using the skin of the human eyelid infiltrated with urea as described above.

  In the first or second embodiment of the present invention, in the near infrared ray reflected from the object, the near infrared ray (and 1850 nm) including at least one wavelength in the range of 1950 nm to 2000 nm among the near infrared rays irradiated to the object. The near infrared ray having at least one wavelength in the range of less than 1950 nm is absorbed by urea (and moisture) in the object. That is, the near-infrared spectrum data reflected from the object is a near-infrared object including at least one wavelength in the range from 1950 nm to 2000 nm (and a near-infrared object having at least one wavelength in the range from 1850 nm to less than 1950 nm). Including information on absorption by urea (and moisture). The degree of absorption (absorbance) of near infrared rays depends on the amount of urea (and moisture) in the object. Near-infrared spectral data reflected from the object includes information regarding the amount of urea (and moisture) in the object.

  The near-infrared spectrum data reflected from the object may be the reflection intensity or reflectance of the near-infrared reflected from the sample with respect to the near-infrared wavelength. The near-infrared spectrum data reflected from the object is the reciprocal or reflection of the reflection intensity of the near-infrared reflected from the sample with respect to the near-infrared wavelength in order to express the degree of absorption by urea (and moisture) in the object. The reciprocal of the rate may be (the near infrared reflection intensity or the reciprocal of the reflectivity corresponds to the degree of absorption by urea (and moisture) in the object).

In the stage of evaluating the amount of urea (and moisture) in the subject, the obtained near-infrared spectrum data is used. Here, the obtained near-infrared spectrum data is processed as necessary. For example, when the near-infrared reflectance R (λ) reflected from the sample with respect to the near-infrared wavelength λ is obtained as the near-infrared spectrum data, the reflectance of the near-infrared reflected from the sample with respect to the near-infrared wavelength λ. The logarithm log (1 / R (λ)) of the reciprocal of R (λ) may be calculated. Note that the logarithm of the reciprocal of the reflectance of near-infrared is known to be an amount related to the absorption characteristics of near-infrared from the Kubelka-Munk theory. In addition, for the spectrum of the logarithm log (1 / R (λ)) of the reciprocal of the near-infrared reflectivity for the near-infrared wavelength λ, in order to correct the baseline offset and slope to zero, the near-infrared reflectivity A double differential d ² log (1 / R (λ)) / dλ ² of the near infrared wavelength λ of the inverse logarithm log (1 / R (λ)) may be calculated. Alternatively, with respect to the spectrum of the logarithm log (1 / R (λ)) of the reciprocal of the near-infrared reflectance with respect to the near-infrared wavelength λ, a known MSC (Multiplicative Scatterer is used to correct the baseline offset and slope to zero. (Correction, multiple scattering correction) may be used. Note that there is a strong correlation between the obtained near-infrared spectrum data (logarithm of the reciprocal of the reflectance of the near-infrared ray) and near-infrared spectrum data to which the second-order differentiation by wavelength and / or MSC processing is applied. It is known that there is near infrared spectrum data obtained by applying a second derivative by wavelength and / or MSC processing instead of the obtained near infrared spectrum data (logarithm of the reciprocal of the reflectance of near infrared rays). Can be used.

  In the near-infrared spectrum data or the processed near-infrared spectrum data obtained in this way, the near-infrared light including at least one wavelength in the range of 1950 nm to 2000 nm (and at least one wavelength in the range of 1850 nm to less than 1950 nm) The value (corresponding peak height, area, etc. in the spectrum) that depends on the degree of absorption by urea (and moisture) in the subject, ie the amount of urea (and moisture) in the subject To do. Therefore, the relative value of the amount of urea (and moisture) in the object can be obtained from the obtained near-infrared spectrum data or processed near-infrared spectrum data. Then, using the relative value of the amount of urea (and water) in the obtained object, the amount of urea (and water) in the object can be evaluated.

  In addition, the shift of at least one wavelength in the range from 1850 nm to less than 1950 nm in the obtained near-infrared spectrum data or processed near-infrared spectrum data depends on the degree of freedom of moisture in the object. Specifically, when there are many free water molecules having a high degree of freedom in the object, the vibration of the free water molecules is not suppressed, so that the frequency of the water molecules increases as a whole. Therefore, at least one wavelength in the range from 1850 nm to less than 1950 nm is shortened. On the other hand, when there are many bound water molecules having a low degree of freedom in the object, the vibration of the bound water molecules is suppressed, so that the frequency of the water molecules is reduced as a whole. Therefore, at least one wavelength in the range from 1850 nm to less than 1950 nm is long. Therefore, by calculating the shift of at least one wavelength in the range of 1850 nm or more and less than 1950 nm in the obtained near-infrared spectrum data or processed near-infrared spectrum data, the degree of freedom of moisture in the object can be evaluated. it can.

  Evaluation of the amount of urea (and moisture) in the subject includes measurement of the amount of urea (and moisture) in the subject (calculation of relative values), comparison and correlation of the amount of urea and moisture in the subject, urea (and Including diagnosis of the subject regarding the amount of water). A single assessment of the amount of urea (and moisture) in the subject may be performed, and multiple assessments of the amount of urea (and moisture) in the subject may be performed over time. In the latter case, the change over time in the amount of urea (and moisture) in the subject can be evaluated. For example, the relative value of the amount of urea that has penetrated into human shark skin and the amount of water that has been retained in human shark skin can be obtained, and urea that has penetrated into human shark skin and retained in human shark skin The time change (correlation) of the amount of the obtained water can be evaluated. It is also possible to diagnose the degree of urea penetration and moisture retention in human shark skin. In addition, the results of evaluation of the amount of urea (and moisture) in the resulting subject were developed in the development of cosmetics or pharmaceuticals containing urea, and the effects of cosmetics or pharmaceuticals containing urea on human eyelid skin in vivo. It can be used to confirm with.

  In addition, a single evaluation of the degree of freedom of moisture in the subject may be performed, and a plurality of evaluations of the degree of freedom of moisture in the subject may be performed over time. In the latter case, it is possible to evaluate the change over time in the degree of freedom of moisture in the subject. For example, in addition to the time variation of the amount of urea permeated into human shark skin and the amount of water retained in human shark skin, the time variation of the degree of freedom of moisture retained in human shark skin is evaluated. be able to.

  In the apparatus for evaluating the amount of urea (and moisture) in the object, the object is irradiated with near infrared rays including at least one wavelength in the range from 1950 nm to 2000 nm (and at least one wavelength in the range from 1850 nm to less than 1950 nm). The means to perform is not particularly limited as long as the object can be irradiated with near infrared rays including at least one wavelength in the range of 1950 nm to 2000 nm (and at least one wavelength in the range of 1850 nm to less than 1950 nm). The means for irradiating near infrared rays including at least one wavelength in the range from 1950 nm to 2000 nm (and at least one wavelength in the range from 1850 nm to less than 1950 nm) is, for example, at least one wavelength in the range from 1950 nm to 2000 nm (And at least one wavelength in the range of 1850 nm or more and less than 1950 nm), a light source that emits near infrared rays, and a member such as an optical fiber that can guide the near infrared rays emitted from the light source and irradiate the target Have. The light source may be a near-infrared source that emits near-infrared light including both at least one wavelength in the range of 1950 nm to 2000 nm and at least one wavelength in the range of 1850 nm to less than 1950 nm. The light source includes a near infrared source that emits a near infrared ray including at least one wavelength in a range of 1950 nm to 2000 nm, and a near infrared source that emits a near infrared ray including at least one wavelength in a range of 1850 nm to less than 1950 nm. It may be.

  For example, to assess the amount of urea (and moisture) in human shark skin, at least one wavelength in the range of 1950 nm to 2000 nm up to the depth of the stratum corneum thickness (30 μm) in human shark skin (And at least one wavelength in the range from 1850 nm to less than 1950 nm) including a light source that emits near infrared rays and an optical fiber that can emit near infrared rays emitted from the light source may be used.

  The means for obtaining the near infrared spectrum data reflected from the object is not particularly limited as long as the near infrared spectrum data reflected from the object can be obtained. The means for obtaining near-infrared spectrum data reflected from the object is, for example, a spectroscope that divides the received near-infrared light, receives at least part of the near-infrared light reflected from the object, and is reflected from the object. A member such as an optical fiber capable of guiding at least a part of the light to the spectroscope, and a means capable of acquiring the wavelength of the dispersed near-infrared light and the intensity of the dispersed near-infrared light. Here, the spectroscope that splits the received near-infrared light can be used to analyze the received near-infrared light such as a diffraction grating, an interference filter, a spectroscopic means such as AOTF (acousto-optic dispersion spectroscopy), and Fourier transform spectroscopy. Including means for spectroscopic analysis.

  Furthermore, if the means for evaluating the amount of urea and the amount of water in the target using near infrared spectrum data can evaluate the amount of urea and the amount of water in the target using near infrared spectral data, There is no particular limitation. The means for evaluating the amount of urea and the amount of water in the object using near infrared spectrum data can evaluate the amount of urea and the amount of water in the object using, for example, near infrared spectrum data. It may be a computer.

  Further, the computer-readable recording medium is not particularly limited as long as the program is recorded. The computer-readable recording medium may be, for example, a magnetic recording medium such as a magnetic disk and an optical recording medium such as an optical disk, on which a computer can read a program.

  FIG. 1 is a diagram illustrating an example of an apparatus for evaluating the amount of urea (and moisture) in a subject according to the present invention.

  The apparatus for evaluating the amount of urea (and moisture) in the subject according to the present invention illustrated in FIG. 1 includes a light source 10, a fiber tip 20, a light projecting optical fiber 30, a light receiving optical fiber 40, a spectral detection unit 50, and a micro. A computer 60 is included. The light projecting optical fiber 30 is connected to the light source 10, and the light receiving optical fiber 40 is connected to the spectroscopic detection unit 50. The spectroscopic detection unit 50 is further connected to the microcomputer 60. The light projecting optical fiber 30 and the light receiving optical fiber 40 penetrate the fiber tip 20 on the opposite side of the light source 10 and the spectral detection unit 50, respectively.

  The light source 10 is a near-infrared source capable of emitting near-infrared rays including a wavelength in the range of 1950 nm to 2000 nm and a wavelength in the range of 1850 nm to less than 1950 nm, preferably a continuous range of wavelengths from 1850 nm to 2050 nm. It is a single light source that emits near infrared rays including Specifically, a lamp such as a tungsten halogen lamp or a tungsten lamp that emits near infrared rays including a continuous wavelength can be used as the light source 10.

  Near-infrared rays emitted from the light source 10 are applied to the skin 100 of the subject's eyelid to which urea has been applied by applying cosmetics containing urea from the fiber tip 20 through the light projecting fiber 30. Near-infrared rays irradiated on the skin 100 of the subject are diffusely reflected toward the fiber tip 20. Part of the near infrared light diffusely reflected by the skin 100 of the subject's eyelid is incident on the light receiving fiber 40. In FIG. 1, the fiber tip 20 and the subject's eyelid skin 100 are separated from each other, but when using the device according to the present invention, the fiber tip 20 is actually attached to the subject's eyelid skin 100. Can also be contacted.

  FIG. 2 is a diagram for explaining an example of the arrangement of light projecting fibers and light receiving fibers at the fiber tip. As illustrated in FIG. 2, on the surface of the fiber tip 20 on the skin 100 side of the subject's eyelid, around the end surface 45 of one light receiving optical fiber 40, there are eight light projecting optical fibers 30. The end surfaces 35 are uniformly arranged in all directions. By arranging the end face 35 of the light projecting optical fiber evenly on the four sides of the end face 45 of the light receiving optical fiber 40, the near-infrared light from the light source 10 can be evenly applied to the skin 100 of the subject's eyelid. The diameter of the end face 35 of the light projecting optical fiber 30 and the diameter of the end face 45 of the light receiving optical fiber are, for example, 300 μm. The length of the light projecting optical fiber 30 is, for example, 1.2 m. By using such an apparatus, the amount of urea and moisture in the skin 100 of the subject's eyelid can be measured.

  As illustrated in FIG. 1 again, a part of the near infrared rays diffusely reflected on the surface of the skin 100 of the subject's eyelid is sent to the spectroscopic detection unit 50 through the light receiving optical fiber 30. Near-infrared light incident through the light-receiving optical fiber 30 is split by a spectroscope provided inside the spectroscopic detection unit 50 and similarly split by a near-infrared detector provided inside the spectroscopic detection unit 50. Near-infrared intensity is detected. The spectroscopic detection unit 50 can detect and detect near infrared rays including wavelengths in the range of 1800 nm to 2050 nm. For the spectroscope used in the spectroscopic detection unit 50, for example, a diffraction grating can be used. As a detector used in the spectroscopic detection unit 50, a detector using a photodiode having a normal temperature InGaAs or the like can be used.

  Next, the near-infrared diffuse reflection spectrum (measured value of wavelength and intensity) detected by the spectroscopic detection unit 50 is converted into an electric signal corresponding to the intensity of the diffuse reflection spectrum by the detector, and the electric signal is amplified. And output to the microcomputer 60 as diffuse reflection spectrum data of near infrared rays. In the microcomputer 60, the relative values of the amounts of urea and water in the skin 100 of the subject's eyelid are calculated using the obtained near-infrared diffuse reflection spectrum data. In this way, the amount of urea and moisture in the skin 100 of the subject's eyelid can be evaluated.

  FIG. 3 is a flowchart illustrating an example of a method for evaluating the amount of urea (and moisture) in a subject according to the present invention.

  The method for evaluating the amount of urea (and moisture) in an object according to the present invention illustrated in FIG. 3 includes the step of applying urea to the object (S1), and the step of irradiating the object with near infrared rays in a predetermined wavelength range (S2). ), Receiving the near-infrared light reflected from the object (S3), obtaining the near-infrared spectrum by dispersing the received near-infrared light (S4), and urea (and moisture) of the object from the obtained spectrum data A step of calculating the amount (S5), and a step of outputting the amount of urea (and moisture) in the obtained object (S6). In the following, it is assumed that the subject is the skin of a human eyelid.

  First, in the step (S1) of applying urea to a subject, a cosmetic or pharmaceutical product containing urea is applied to the skin of a human eyelid as the subject. The skin of the eyelids to which the cosmetics or medicines containing urea are applied may be covered with a plastic film so that the urea contained in the cosmetics or medicines sufficiently penetrates the skin of the human eyelids. In this case, after a predetermined time has elapsed, the plastic film is removed from the skin of the eyelids, and the cosmetics or pharmaceuticals remaining on the surface of the eyelid skin are thoroughly wiped with paper.

  Next, in the step (S2) of irradiating the object with near infrared rays in a predetermined wavelength range, the amount of urea (and moisture) in the object according to the present invention as exemplified in FIGS. Skin of the eyelid which permeated urea from cosmetics or pharmaceuticals with near infrared rays including at least one wavelength in the range of 1950 nm to 2000 nm (and at least one wavelength in the range of 1850 nm to less than 1950 nm) using the apparatus to be evaluated Irradiate. For example, near infrared rays including wavelengths in the range from 1800 nm to 2050 nm emitted from the light source 10 are transmitted from the end face 35 of the projecting optical fiber to the skin 100 of the eyelid through the projecting optical fiber 30. Irradiate.

  Next, in the step of receiving near infrared rays reflected from the object (S3), at least one wavelength in the range from 1950 nm to 2000 nm diffusely reflected from the skin of the eyelid (and at least one in the range from 1850 nm to less than 1950 nm). Near-infrared light including two wavelengths. For example, near infrared rays including a wavelength in the range of 1800 nm to 2050 nm diffusely reflected from the skin 100 of the eyelid are received by the end face 45 of the light receiving optical fiber at the fiber tip 20. Near-infrared light received at the end face 45 of the light receiving optical fiber is guided to the spectroscopic detection unit 50 through the light receiving optical fiber 40.

  Next, in the step of obtaining a near-infrared spectrum by splitting the received near-infrared light (S4), the received near-infrared light is spectrally separated by a spectroscope such as a diffraction grating included in the spectral detection unit 50, and the near-infrared spectrum is obtained. Is detected by a detector included in the spectroscopic detection unit 50. The near-infrared wavelength spectrally separated by the spectroscope and the near-infrared intensity value detected by the detector constitute the diffuse reflectance spectrum data of the near-infrared diffusely reflected from the skin of the eyelid. The near-infrared wavelength and intensity data constituting the near-infrared diffuse reflection spectrum data is transmitted to the microcomputer 60 as an electrical signal.

Next, in the step (S5) of calculating the amount of urea (and moisture) in the object from the obtained spectrum data, the microcomputer 60 converts the near-infrared diffuse reflection spectrum data diffusely reflected from the skin of the eyelid. The following processing is performed. The near-infrared reflectance R (λ) with respect to the near-infrared wavelength λ is calculated by normalizing the near-infrared intensity distribution in the near-infrared diffuse reflection spectrum data diffusely reflected from the skin of the eyelid. Next, in order to represent the near-infrared absorption as a peak and logarithmically, a logarithm log (1 / R (λ)) of the reciprocal of the near-infrared reflectance with respect to the near-infrared wavelength λ is calculated. Further, in order to correct the offset and slope of the baseline in the logarithm log (1 / R (λ)) of the reciprocal of the near-infrared reflectance with respect to the near-infrared wavelength λ, the logarithm of the reciprocal of the near-infrared reflectance is corrected to zero. Log (1 / R (λ)) is differentiated twice with the near infrared wavelength λ. Thereby, the difference etc. by the difference in the object regarding the scattering characteristic of near infrared rays can be reduced. In this way, a double differential d ² log (1 / R (λ)) / dλ ² of the logarithm of the reciprocal of the near-infrared reflectance with respect to the near-infrared wavelength λ is obtained. Here, the value of the above-mentioned second derivative at a wavelength in the range from 1950 nm to 2000 nm (and a wavelength in the range from 1850 nm to less than 1950 nm) is the relative value of the amount of urea that has penetrated into the skin of the eyelid (and the skin of the eyelid). Relative value of the amount of water held in the

  Finally, in the step of outputting the amount of urea (and moisture) in the obtained subject (S6), the relative value of the amount of urea that has penetrated into the obtained eyelid skin (and the amount of moisture retained in the eyelid skin) The relative value of the quantity is output to an output device such as a monitor of the microcomputer 60. In this way, from the relative value of the amount of urea that permeated into the obtained eyelid skin (and the relative value of the amount of water retained in the eyelid skin), the urea (and moisture) content in human eyelid skin The amount can be evaluated.

  Further, in the near-infrared diffuse reflection spectrum data that can be obtained in the step (S4) of spectrally analyzing the received near-infrared light to obtain a near-infrared spectrum, from the shift in the wavelength of absorption by moisture in the range from 1850 nm to less than 1950 nm, It is also possible to evaluate the degree of freedom of water molecules held on the skin of a human eyelid.

FIG. 4 is a flowchart illustrating an example of a program according to the present invention. The program according to the present invention illustrated in FIG. 4 is similar to steps S2 to S6 described above, the step of irradiating the object with near-infrared rays (T1), the step of receiving near-infrared light reflected from the object (T2), and the light reception The near-infrared spectrum (reflectance R (λ) with respect to wavelength) to obtain a near-infrared spectrum (T3), and reflectivity R (λ) with respect to wavelength from d ² log (1 / R (λ)) / dλ ² (T4), a step (T5) of calculating a relative value of the amount of urea (and moisture) from d ² log (1 / R (λ)) / dλ ² , urea (and The computer executes the step (T6) of outputting the relative value of the amount of water. Note that the step of outputting the shift of the wavelength of absorption by moisture in the range of 1850 nm or more and less than 1950 nm in the near-infrared diffuse reflection spectrum data may be executed by a computer.

  The program according to the present invention as exemplified in FIG. 4 is recorded on a computer-readable recording medium. Again, as illustrated in FIG. 1, the program according to the present invention as illustrated in FIG. 4 is recorded in a recording medium 70, for example. The recording medium 70 is, for example, a magnetic recording medium such as a magnetic disk or an optical recording medium such as an optical disk.

  Next, embodiments of the present invention will be described with reference to the drawings.

  In the present embodiment, the amount of urea and water in the object illustrated in FIG. 3 is evaluated using the apparatus for evaluating the amount of urea and water in the object illustrated in FIGS. 1 and 2 and the program illustrated in FIG. According to the method, after applying cosmetics containing urea to human shark skin, the time variation of the amount of urea penetrated into the human shark skin, the time variation of the amount of water retained in the human shark skin, human The time change of the degree of freedom of water molecules held in the skin of the cocoon was evaluated. In addition, as a control group, without applying urea-containing cosmetics to human eyelid skin, the time change in the amount of urea in human eyelid skin, the time change in the amount of water in human eyelid skin, The time change of the degree of freedom of water molecules in the shark skin was evaluated.

  The apparatus for evaluating the amount of urea and water in the object used in this example is an apparatus for evaluating the amount of urea and water in the object as illustrated in FIGS. 1 and 2. As a light source to which the light projecting optical fiber and the light receiving optical fiber illustrated in FIGS. 1 and 2 are connected, Spectron Tech Co. capable of emitting near infrared rays including wavelengths in the range of 1250 nm to 2250 nm. Ltd .. A Fiber Probe (diffuse reflection type probe) manufactured by (Korea) was used. Moreover, the FT-NIR spectrometer VIR-9500 made from JASCO was used as the spectroscopic detection unit illustrated in FIG. This apparatus can obtain a near-infrared spectrum in a wavelength range from 1250 nm to 2250 nm and a wavelength interval (resolution) of about 1 nm. Further, a commercially available personal computer was used as the microcomputer illustrated in FIG. As shown in FIGS. 1 and 2, Spectron Tech Co. as a light source to which a light projecting optical fiber and a light receiving optical fiber are connected. Ltd .. (Korea) Fiber Probe connected to JASCO's FT-NIR spectrometer VIR-9500 as a spectroscopic detection unit, and JASCO's FT-NIR spectrometer VIR-9500 as a spectroscopic detection unit Connected to a commercially available computer as a computer.

  The subject is the skin of the foot heels of five men and women in their 20s to 50s. 0.5 g of urea-containing cream “Ferzea (registered trademark) HA20 cream” (manufactured by Shiseido Co., Ltd.) was applied to the skin of one heel (30 mm × 30 mm region) of the feet of five people. After the cream containing urea was applied to the skin of wrinkles, the area of the skin of wrinkles to which the cream containing urea was applied was covered with a transparent plastic film. At the same time, as the subject group, the skin of the other eyelid on the feet of five people was covered with a transparent plastic film. Two hours after the area of the skin of the eyelid to which the cream containing urea was applied was covered with a transparent plastic film, the transparent plastic film covering the eyelids of both feet was removed, and the area of the eyelid to which the cream containing urea was applied was removed. The urea-containing cream remaining on the surface area of the skin was wiped off sufficiently (to the extent that the presence of the urea-containing cream could not be visually confirmed). Then, the time change of the amount of urea in the skin of the heel of both feet, the time change of the amount of water in the skin of the heel, and the time change of the degree of freedom of water molecules in the skin of the heel were measured. Each measurement was performed at 1, 2, 4, and 24 hours after wiping off the urea-containing cream. Before applying the above cream containing urea to the skin of the heel, the amount of urea in the skin of the heel of both feet, the amount of water in the skin of the heel of both feet, and the degree of freedom of water molecules in the skin of the heel of both feet are measured in advance. I kept it.

  Here, as shown in FIG. 3, the skin of the heels of both feet is irradiated with near infrared light including a wavelength ranging from 1250 nm to 2250 nm, and a part of the near infrared light diffusely reflected from the skin of the heels of both feet is received. The near-infrared light received was split and the wavelength and intensity of the split light were detected. From the detected near-infrared wavelength and intensity data, the detected near-infrared wavelength λ and reflectance R (λ) data were calculated. From the data of the near-infrared wavelength λ and the reflectance R (λ), the relationship between the near-infrared wavelength λ and the logarithm log (1 / R (λ)) of the reciprocal of the reflectance (in this embodiment, the near-infrared Will be referred to as the spectrum).

  FIG. 5 is a diagram showing the time change of the near-infrared spectrum obtained in the example of the present invention, and (a) shows the time change of the near-infrared spectrum for the skin of the eyelid to which the urea-containing cream is applied. (B) is a figure which shows the time change of the near-infrared spectrum regarding the skin of the eyelid which did not apply | coat the cream containing urea. 5A and 5B, the horizontal axis represents the near-infrared wavelength (nm) (1250 nm to 2250 nm), and the vertical axis represents log (1 / reflectance R) (arbitrary unit). 5 (a) and 5 (b) show the measurement results before and after applying urea-containing cream on the skin of one eyelid out of five. As shown in FIG. 5 (b), the near-infrared spectrum of the skin of the wrinkles that had not been applied with the urea cream was almost unchanged before and after the urea cream was applied to the other wrinkles. On the other hand, as shown in FIG. 5 (a), the near-infrared spectrum related to the skin of the wrinkle to which the urea-containing cream is applied is based on the near-infrared spectrum before the urea-containing cream is applied to the wrinkle. After the cream containing urea is applied to the candy, the log (1 / R) value increases after 1 hour, and the log (1 / R) value decreases after 4 hours, and the cream containing urea is applied to the candy. It was confirmed to approach the near-infrared spectrum in the previous.

Next, from the logarithm log (1 / R (λ)) of the reciprocal of the wavelength λ of the near infrared ray and the logarithm of the reciprocal of the reflectivity with respect to the wavelength λ of the near infrared ray d ² log (1 / R ( λ)) / dλ ^2, and is related to the double differential d ² log (1 / R (λ)) / dλ ² of the logarithm of the reciprocal of the wavelength λ and the reflectance of the near-infrared (in this example, This will be referred to as an infrared double differential spectrum).

FIG. 6 is a diagram showing the time change of the near-infrared second-order derivative spectrum obtained in the example of the present invention. FIG. 6 (a) is the near-infrared second-order derivative relating to the skin of salmon coated with urea-containing cream. It is a figure which shows the time change of a spectrum, (b) is a figure which shows the time change of the near-infrared second derivative spectrum regarding the skin of the eyelid which did not apply | coat the cream containing urea. 6A and 6B, the horizontal axis represents the near-infrared wavelength λ (nm) (1850 nm to 2050 nm), and the vertical axis represents d ² log (1 / reflectance R) / dλ ² (arbitrary). Unit). 6 (a) and 6 (b) show the measurement results of the skin of one eyelid out of 5 before and after application of urea-containing cream, after 1, and 24 hours. As shown in FIG. 6 (b), the near-infrared second-order derivative spectrum of the skin of the wrinkles that had not been applied with the urea-containing cream hardly changed before and after the urea-containing cream was applied to the other wrinkles. On the other hand, as shown in FIG. 6 (a), the near-infrared second-order derivative spectrum of the skin of the wrinkle applied with the cream containing urea is the second-order derivative of the near-infrared before applying the cream containing urea to the wrinkle. Based on the spectrum, one hour after applying the urea-containing cream on the candy, the value of d ² log (1 / R)) / dλ ² in the range from 1850 nm to less than 1950 nm and d ² in the range from 1950 nm to 2000 nm. the value of log (1 / R)) / dλ 2 is the absolute value becomes larger as a negative. Here, when the value of d ² log (1 / R)) / dλ ² is negative and the absolute value is large, the function of log (1 / R) with respect to λ is convex upward. This corresponds to the fact that the convex portion is not sharp. Since log (1 / R) corresponds to near-infrared absorption, the value of d ² log (1 / R)) / dλ ² is negative and the absolute value is increased to 1850 nm. This means that the absorption in the range below 1950 nm and the absorption in the range from 1950 nm to 2000 nm increase. This also means that one hour after the urea cream was applied to the cocoon, urea penetrated the cocoon skin and the moisture was retained in the cocoon skin by the penetration of urea into the cocoon skin. . At 4 hours after the urea cream was applied to the candy, the value of d ² log (1 / R)) / dλ ² in the range from 1850 nm to 1950 nm was 2 The value of d ² log (1 / R)) / dλ ² in the range from 1950 nm to 2000 nm is close to the second derivative spectrum. It was the same. This means that in 4 hours after the cream containing urea is applied to the eyelids, the urea that has penetrated the eyelid skin is retained in the eyelid skin, but the moisture retained in the eyelid skin is reduced. means. Further, the near-infrared second-order differential spectrum of 24 hours after the urea-containing cream was applied to the cocoon was close to the near-infrared near-infrared second-derivative spectrum before the urea-containing cream was applied to the candy. Furthermore, in one hour after the urea-containing cream is applied to the candy, the wavelength corresponding to the minimum value of d ² log (1 / R)) / dλ ² in the range of 1850 nm or more and less than 1950 nm is applied to the candy cream. Before and after applying the urea-containing cream to the wrinkle, it shifted to a shorter wavelength compared to after 2 hours. Compared to before applying the urea cream to the candy, one hour after applying the urea cream to the candy, the percentage of free water molecules in the skin increases, and the urea cream is applied to the candy. After 2 hours, the ratio of free water molecules present in the skin decreases, which means that the ratio of free water molecules before the urea-containing cream is applied to the bag is approached.

As shown in FIG. 6 (a), the value of d ² log (1 / R)) / dλ ² in the range of 1950 nm to 2000 nm and the range of 1850 nm to less than 1950 nm before and after applying the cream containing urea to the skin of wrinkles Measuring the amount of urea permeated into the skin of the eyelid and the amount of water retained by the urea permeated through the skin by measuring the change in the value of d ² log (1 / R)) / dλ ² at It was confirmed that it was possible. Further, after applying the urea-containing cream to the skin of the ^{heel, d 2 log (1 / R} ) at 2000nm the range above 1950 nm) / d [lambda] ² values and ^d in the range of less than 1950 nm or 1850nm 2 log (1 / R )) / by measuring the time variation of the d [lambda] ² values, it was confirmed that it is possible to evaluate the time variation of urea and the amount of moisture in the skin of the heel. Furthermore, to evaluate the degree of freedom of water molecules in the skin of wrinkles by measuring the shift of the wavelength corresponding to the minimum value of d ² log (1 / R)) / dλ ² in the range from 1850 nm to less than 1950 nm It was confirmed that

FIG. 7 is a diagram showing the change over time in the amount of urea in the skin of the eyelid obtained in the example of the present invention. In FIG. 7, the horizontal axis is the time (hours) after applying the urea-containing cream (including before applying the urea-containing cream), and the vertical axis is d ² log (1 in the range from 1950 nm to 2000 nm). / R)) / dλ ² minimum of 5 people average. Further, the solid line is the minimum value of d ² log (1 / R)) / dλ ² relating to the skin of the eyelids to which the cream containing urea is applied, and the dotted line is d ² relating to the skin of the eyelids not applied with the cream containing urea. log (1 / R)) is a minimum value of / d [lambda] ^2. Compared with before applying the urea-containing cream, one hour after applying the urea-containing cream, the minimum value of d ² log (1 / R)) / dλ ² in the range from 1950 nm to 2000 nm is negative. With that, the absolute value increased. That is, one hour after applying the cream containing urea, the absorption by urea in the skin of the cocoon increased, indicating that urea penetrated into the skin of the cocoon. Also, the minimum value of ^{d 2 log (1 / R)} ) / dλ 2 in 2 hours after applying the urea-containing cream, ^d in 1 hour after the application of the urea-containing cream 2 log (1 / R)) / dλ is the same as the ^second minimum value, the 4 hours after applying the urea-containing cream minimum value of ^{d 2 log (1 / R)} ) / dλ 2 decreased. This is because urea that has penetrated into the skin of the cocoon is retained for 1 to 2 hours after the application of the cream containing urea, and urea that has penetrated into the skin of the cocoon after 4 hours of application of the cream containing urea. Indicates a decrease.

FIG. 8 is a diagram showing the change over time in the amount of water in the skin of the eyelid obtained in the example of the present invention. In FIG. 8, the horizontal axis represents the time (hours) after applying the urea-containing cream (including before applying the urea-containing cream), and the vertical axis represents d ² log (1 in the range of 1850 nm or more and less than 1950 nm). / R)) / dλ ² minimum of 5 people average. Further, the solid line is the minimum value of d ² log (1 / R)) / dλ ² relating to the skin of the eyelids to which the cream containing urea is applied, and the dotted line is d ² relating to the skin of the eyelids not applied with the cream containing urea. log (1 / R)) is a minimum value of / d [lambda] ^2. Compared with before applying the cream containing urea, 1 hour and 2 hours after applying the cream containing urea, the minimum value of d ² log (1 / R)) / dλ ² in the range from 1850 nm to less than 1950 nm is As well as being negative, its absolute value increased. That is, in one hour after the application of the cream containing urea, the absorption due to moisture in the skin of the eyelids increased, indicating that moisture was retained in the skin of the eyelids by the application of urea. Further, the minimum value of d ² log (1 / R)) / dλ ² decreased 4 hours after the urea-containing cream was applied. This is because 1 to 2 hours after applying the cream containing urea, the moisture permeates the skin of the wrinkle by urea that has penetrated into the skin of the wrinkle, and 4 hours after applying the cream containing urea, It shows that the water | moisture content hold | maintained decreased.

FIG. 9 is a diagram for explaining the change over time in the degree of freedom of moisture in the skin of the eyelid obtained in the example of the present invention. In FIG. 8, the horizontal axis represents the time (hours) after applying the urea-containing cream (including before applying the urea-containing cream), and the vertical axis represents d ² log (1 in the range of 1850 nm or more and less than 1950 nm). / R)) / dλ average over five wavelengths corresponding to the minimum value of ² is (nm). The solid line is the wavelength corresponding to the minimum value of d ² log (1 / R)) / dλ ² for the skin of the cocoon to which the cream containing urea is applied, and the dotted line is the wavelength of the cocoon that has not been applied with the cream containing urea. This is the wavelength corresponding to the minimum value of d ² log (1 / R)) / dλ ^{2 for} the skin. Compared to before applying the cream containing urea, the wavelength corresponding to the minimum value of d ² log (1 / R)) / dλ ² is 1 to 4 hours after applying the cream containing urea. Shifted to. On the other hand, at 24 hours after applying the cream containing urea, the wavelength corresponding to the minimum value of d ² log (1 / R)) / dλ ² is d ² log (1 / R) before applying the cream containing urea. ) closer to the corresponding wavelength to the minimum value of / dλ ^2. This is because urea penetrated into the shark's skin increases the proportion of free water molecules in the shark's skin, and then decreases the proportion of free water molecules in the shark's skin. It shows approaching the proportion of free water molecules before application.

  Although the embodiments and examples of the present invention have been specifically described above, the present invention is not limited to these embodiments and examples, and these embodiments and examples of the present invention are not limited thereto. Can be changed or modified without departing from the spirit and scope of the present invention.

It is a figure explaining the example of the apparatus which evaluates the quantity of urea (and water | moisture content) in the object by this invention. It is a figure explaining an example to arrangement | positioning of the fiber for light projection and the fiber for light reception in a fiber front-end | tip part. 6 is a flowchart illustrating an example of a method for evaluating the amount of urea (and moisture) in a subject according to the present invention. It is a flowchart explaining the example of the program by this invention. It is a figure which shows the time change of the near-infrared spectrum obtained in the Example of this invention, and is a figure which shows the time change of the near-infrared spectrum regarding the skin of the eyelid which apply | coated the cream containing urea. It is a figure which shows the time change of the near-infrared spectrum obtained in the Example of this invention, and is a figure which shows the time change of the near-infrared spectrum regarding the skin of the eyelid which did not apply | coat the cream containing urea. It is a figure which shows the time change of the 2nd derivative spectrum of the near infrared ray obtained in the Example of this invention, and is a figure which shows the time change of the 2nd derivative spectrum of the near infrared ray regarding the skin of the eyelid which apply | coated the cream containing urea. . It is a figure which shows the time change of the 2nd derivative spectrum of the near infrared ray obtained in the Example of this invention, and is a figure which shows the time change of the 2nd derivative spectrum of the near infrared ray regarding the skin of the eyelid which did not apply | coat the cream containing urea. It is. It is a figure which shows the time change of the urea amount in the skin of the eyelid obtained in the Example of this invention. It is a figure which shows the time change of the moisture content in the skin of the eyelid obtained in the Example of this invention. It is a figure explaining the time change of the freedom degree of the water | moisture content in the skin of the eyelid obtained in the Example of this invention.

Explanation of symbols

DESCRIPTION OF SYMBOLS 10 Light source 20 Fiber front-end | tip part 30 Optical fiber for light emission 35 End surface of the optical fiber for light emission 40 Optical fiber for light reception 45 End surface of the optical fiber for light reception 50 Spectroscopic detection unit 60 Microcomputer 70 Recording medium 100 Skin of the eyelid

Claims (9)

Irradiating an object with near infrared rays including at least one wavelength in a range of 1950 nm to 2000 nm,
Obtaining the amount of urea in the object comprising: obtaining the near-infrared spectral data reflected from the object; and using the infrared spectral data to evaluate the amount of urea in the object. How to evaluate.   2. The method for assessing the amount of urea in a subject according to claim 1, wherein the subject is human eyelid skin. Irradiating the object with near infrared light including at least one wavelength in the range of 1850 nm to 1950 nm and at least one wavelength in the range of 1950 nm to 2000 nm;
Obtaining the near-infrared spectral data reflected from the object, and using the near-infrared spectral data to evaluate the amount of urea and the amount of moisture in the object. To evaluate the amount of urea and moisture in   4. The method for assessing the amount of urea and water in a subject according to claim 3, wherein the subject is human eyelid skin. Irradiating an object with near infrared rays including at least one wavelength in a range of 1950 nm to 2000 nm,
A program for causing a computer to execute the steps of obtaining the near-infrared spectrum data reflected from the object and evaluating the amount of urea in the object using the near-infrared spectrum data. Irradiating the object with near infrared rays including at least one wavelength in the range of 1850 nm or more and less than 1950 nm and at least one wavelength in the range of 1950 nm or more and 2000 nm or less;
Obtaining the near-infrared spectrum data reflected from the object; and causing the computer to execute a step of evaluating the amount of urea and the amount of moisture in the object using the near-infrared spectrum data. Program to do.   A computer-readable recording medium on which the program according to claim 5 or 6 is recorded. Means for irradiating an object with near infrared rays including at least one wavelength in the range of 1950 nm to 2000 nm;
Means for obtaining near infrared spectrum data reflected from the object, and means for evaluating the amount of urea in the object using the near infrared spectrum data; Device to evaluate. Means for irradiating an object with near infrared rays including at least one wavelength in the range of 1850 nm or more and less than 1950 nm and at least one wavelength in the range of 1950 nm or more and 2000 nm or less;
Means for obtaining the near-infrared spectrum data reflected from the object, and means for evaluating the amount of urea and the amount of water in the object using the near-infrared spectrum data For evaluating the amount of urea and moisture in the plant.

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