JP2006111560A - Ceramide synthesis promoter - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To obtain a ceramide synthesis promoter comprising one or more kinds selected from extracts of Oryza sativa L., Pueraria lobata ohwii, Prunus armeniaca L. var. ansu Maximowicz, Lonicera japonica Thunberg, Saxifraga stolonifera, Rubus suavissimus Shugan Lee, Apocynum venetum L., Crataegus cuneata, Rosa roxburghii Traff. f. normalis Rehd. et Wils, Acanthopanax senticosus Harms, Zizyphus jujuba var. inermis Rehd, Perilla frutescens crispa, Coptis japonica, Asarum sieboldii, Scutellaria baicalensis Georgi, Phellodendron amurense, Morus alba, Paeonia suffruticosa Andrews Paeonia, Paeonia lactiflora Pallas, Citrus unshiu Mercov, Sapindus mukorossi Gaertn, Syzygium aromaticum, Lilium candidum, Glycine max Merrill and Tremella fuciformis B., and a composition that comprises the ceramide synthesis promoter and has preventing or ameliorating effect on chapped skin and dry skin. <P>SOLUTION: The substance comprising one or more kinds selected from extracts of Oryza sativa L., Pueraria lobata ohwii, Prunus armeniaca L. var. ansu Maximowicz, Lonicera japonica Thunberg, Saxifraga stolonifera, Rubus suavissimus Shugan Lee, Apocynum venetum L., Crataegus cuneata, Rosa roxburghii Traff. f. normalis Rehd. et Wils, Acanthopanax senticosus Harms, Zizyphus jujuba var. inermis Rehd, Perilla frutescens crispa, Coptis japonica, Asarum sieboldii, Scutellaria baicalensis Georgi, Phellodendron amurense, Morus alba, Paeonia suffruticosa Andrews Paeonia, Paeonia lactiflora Pallas, Citrus unshiu Mercov, Sapindus mukorossi Gaertn, Syzygium aromaticum, Lilium candidum, Glycine max Merrill and Tremella fuciformis B., has excellent promoting effect on ceramide synthesis. The composition comprising their extracts is safe and has excellent ameliorating effect on chapped skin and dry skin. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention includes rice, kudzu, apricot, honeysuckle, snow flake, tencha, rafma, hawthorn, izayoibara, ezokogi, jujube, perilla, auren, saishin, koganebana, kihada, mulberry, button, peonies, chimpi, mukuroji, clove, lily, The present invention relates to a ceramide synthesis promoter characterized by containing one or more selected from soybean and white jellyfish extracts, and particularly to a composition that exhibits effectiveness in preventing rough skin and dry skin.

  Ceramide is the main component of lamellar granules produced and secreted in the upper spiny layer and granule layer when cells move from the basal layer of the skin to the stratum corneum. Forms keratinocyte lipids that play an important role. Causes of rough skin include dryness, contact with irritants, and skin irritation due to ultraviolet rays. It is said that promoting the production of ceramide is effective in restoring the barrier function that protects the skin from dryness and irritants. It has been confirmed that seven types of ceramide are present in human skin, and it is ideal that all kinds of ceramides are supplemented at a ratio of existing in the stratum corneum.

Conventionally, ceramide has been blended into various skin external preparations as a cosmetic that is effective in preventing and improving rough skin and dry skin, but supplements all types of ceramide present in human skin at an appropriate ratio. That was technically difficult. Therefore, it is considered important to promote ceramide synthesis in vivo. Patent Documents 1 and 2 have been reported as ceramide synthesis accelerators. Rice, Scraps, Apricots, Honeysuckle, Tencha, Tencha, Rafuma, Hawthorn, Izayoi Rose, Ezocogi, Jujube, Perilla, Ouren, Saishin, Scarlet, Yellowfin, Mulberry, Button, Peonies, Chimpi, Mukuroji, Chouji, Lily, Soybean, and Soybean The ceramide synthesis promoting effect of the extract of white jellyfish has not been reported.
JP-A-8-217658 JP 2001-220345 A

  From the above, a ceramide synthesis accelerator excellent in the effect of improving rough skin and dry skin is desired.

  Under these circumstances, as a result of intensive research and investigation, the present inventors have found that rice, kudzu, apricot, honeysuckle, saxifrage, tencha, rafma, hawthorn, izayoibara, ezokogi, jujube, perilla, ouren, saishin, koganebana, kihada, quail , Button, peonies, chimpi, mugwort, clove, lily, soybean, and white jellyfish extracts are found to promote ceramide synthesis, and further to a composition containing one or more selected from these extracts The present inventors have found the effect of preventing and improving rough skin and dry skin, and have completed the present invention.

  The rice used in the present invention is the fruit of Oryza sativa L belonging to the family Gramineae, and brown rice or rice bran can be used.

  Kudu used in the present invention belongs to the leguminous family and has the scientific name: Pueraria lobata ohwi. It is wild from eastern Asia, India, and the tropics of the Malay Peninsula to some temperate zones. Is a perennial. You can use the root part (herbal medicine name: kakkon), etc., excluding the pericarp. Moreover, you may use the related plant which distribute | circulates as a kudzu root.

  The apricot used in the present invention belongs to the family Rosaceae, such as dried apricot (Prunus armenica L), apricot (Prunus armenica L. var. Ans Maximowicz) or other related plant seeds (herbal medicine name: apricot kernel) Can be used.

  The honeysuckle used in the present invention belongs to the family Lonicera, and is a honeysuckle (scientific name: Lonicera Japanpica Thinberg) or other related genus plant, and is a vine plant that grows naturally in Japan, China and the like. It is possible to use things that are picked and chopped with branches, or leaves that are picked and dried (herbal medicine name: Shinobu) or dried flower parts (herbal medicine name: gold and silver flowers).

  The saxifrage used in the present invention belongs to the family Saxifragaceae and has the scientific name: Saxifraga stolonifera (L.) Meerb, which is a perennial herb distributed in Japan, China, and the like. Whole grass can be used.

  The tencha used in the present invention belongs to the family Rosaceae and has the scientific name: Rubus suavissimus Shugan Lee, which is produced only in the mountainous area of southwestern China, and is also called open stomach tea, and is used as an appetite booster, expectorant and cough. The dried leaves (herbal medicine name: tea) can be used.

  Raffma (Rafu hemp, scientific name: Apocynum venetum L.) used in the present invention is a shrub of the Oleander family. It is mainly distributed in the northwestern part of China, and it is possible to use parts of plants such as leaves and stems or dried whole plants.

  The hawthorn used in the present invention belongs to the family Rosaceae and is a hawthorn (Scientific name: Crataegus cuneata Sieb. Et Zucc) or a related plant thereof, and is a deciduous shrub distributed in various provinces of China and Japan. What dried the fruit part (namely, herbal medicine: Yamako) can be used.

  The Izayoi rose used in the present invention belongs to the family Rosaceae and has a scientific name: Rosa roxburghii Traffic. f. normalis Rehd. et Wils. The dried fruit (herbal medicine name: pear) can be used.

  Ezoukogi used in the present invention belongs to the family Uridaceae, and has the scientific name: Acanthopanax senticosus Harms, and is distributed in Hokkaido, Saita, the Korean Peninsula, northern China, and Amur. What dried the root bark (namely, crude drug name: five skins) etc. can be used.

  The jujube used in the present invention belongs to the family Oleaceae, and is a jujube (scientific name: Zizyhus jujuba var. Inermis Rehd) or a related plant thereof, and is a deciduous tree that originates from North America to western Europe. The dried fruit (herbal medicine name: Daegu) can be used.

  The perilla used in the present invention belongs to the family Lamiaceae, and is perilla (Perilla Fruitscens var Acta) or a related plant thereof, and its origin is China. Those obtained by drying leaves (name of crude drugs: Soyo), those obtained by drying seeds (name of crude drugs: Shiso) can be used.

  The auren used in the present invention belongs to the family Ranunculaceae, and is an oren (scientific name: Coptis japonica) or other related plant, and is a forest floor plant distributed from the southwestern Hokkaido to Honshu and Shikoku, with dried rhizomes (name of crude drugs : Henren) etc.

  The saicin used in the present invention is Keirinsaicin (scientific name: Asiasarum heterotropides F.) or Usbasaicin (scientific name: Asiasarum sieboldi F.), which is obtained by drying rhizomes and roots, or rooted whole plants (herbal medicines). Name: Spicy) can be used.

  Scarabae used in the present invention belongs to the family Lamiaceae and has the scientific name: Scutellaria baicalensis Georgi. It is a perennial herb grown in Siberia from northern China and in Gunma and Nara prefectures in Japan. Dry roots (herbal medicine name: yellow rice) can be used. Moreover, you may use the same genus plant distribute | circulated as yellow rice.

  The yellowfin used in the present invention belongs to the citrus family and is yellowfin (Scientific name: Phellodendron amurense Ruprecht) or other related plants, and is a deciduous tree widely distributed in the Amur River, northern China and the Japanese archipelago. The dried bark excluding the pericard (herbal medicine name: jaundice) can be used.

  The mulberry used in the present invention belongs to the mulberry family and is a mulberry (scientific name: Morus alba L.) or other related plant, and is a deciduous tree native to the Amur River, China. What dried the root bark (namely, crude drug name: mulberry skin) etc. can be used.

  The button used in the present invention belongs to the button family, and has the scientific name: Paeonia suffruticosa Andrews Paeonia, which is a deciduous shrub native to northwestern China. What dried the root bark (namely, herbal medicine: peony skin) etc. can be used.

  The peony used in the present invention belongs to the button family, and is a peony (scientific name: Paeonia lactiflora Pallas) or a related plant thereof, and is a perennial herb distributed in Northeast China, East Siberia, and the Korean Peninsula. The dried roots (herbal medicine name: glaze) can be used.

  The chimney used in the present invention belongs to the citrus family, and can be used in the citrus unshiu marcov (scientific name: Citrus unshiu Markov) or a related plant thereof, in which fruit skin is dried (name of crude drug: cheng).

  Mukuroji used in the present invention belongs to the family Mukurodiaceae and has the scientific name: Sapindus mukorossi Gaertn, and is a deciduous tree distributed in Japan, Taiwan, China, India and Nepal. It is possible to use a dried fruit skin (name of crude drug: life-extending skin).

  The clove used in the present invention belongs to the Myrtaceae family and has the scientific name: Syzygium aromaticum Merrill et Perry, which is a deciduous tree distributed in Japan, Taiwan, China, India, Nepal, and dried florets (herbal medicine name: nickname) Etc. can be used.

  The lily used in the present invention belongs to the genus Lily of the family Lilyaceae. For example, Madonna Lily (scientific name: Lilium candidum) or Hakatayuri (scientific name: Lilium brownii FE), Himeyuri (scientific name: Lilium controller S.) Lilium lancifolium T.), dried lily bulb (scientific name: Lilium auratum L.) bulbs (herbal medicine name: lily) and the like can be used.

  The soybean used in the present invention belongs to the leguminous family and has the scientific name: Glycine max Merrill, and is an annual plant native to China. It has been used since ancient times for its gastrointestinal preparation and the effect of spreading congestion. Isoflavones such as Daidzin extracted from seeds or seeds can be used.

  The white jellyfish used in the present invention (scientific name: Tremella fuciformis B.) is a mushroom belonging to the family Asteraceae, and can use fruit bodies, fungus bodies, and the like.

  Rice, crumbs, apricots, honeysuckle, marsh, tencha, rafma, hawthorn, izayoibara, ezokogi, jujube, perilla, lauren, saishin, koganebana, kihada, mulberry, button, peonies, chimpi, mukuroji, choji, lily, soybean The extract of white jellyfish is not particularly limited, and for example, it may be extracted by heating, or may be extracted at normal temperature or low temperature. Examples of the solvent to be extracted include water, lower alcohols (methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, etc.), liquid polyhydric alcohols (1,3-butylene glycol, propylene glycol). , Glycerin, etc.), ketones (acetone, methyl ethyl ketone, etc.), acetonitrile, esters (ethyl acetate, butyl acetate, etc.), hydrocarbons (hexane, heptane, liquid paraffin, etc.), ethers (ethyl ether, tetrahydrofuran, propyl ether) Etc.). Preferred are polar solvents such as water, lower alcohols and liquid polyhydric alcohols, and particularly preferred are water, ethanol, 1,3-butylene glycol and propylene glycol. These solvents may be used alone or in combination of two or more.

  The extract may be used as it is, or may be used after concentration, dilution, filtration, decolorization with activated carbon, deodorization, or the like, if necessary. Further, the extracted solution may be used as a dried product after being concentrated and dried, spray dried, freeze dried, etc., or may be used after concentrating or isolating the active ingredient by performing column purification or the like. good.

  Rice, crumbs, apricots, honeysuckle, marsh, tencha, rafma, hawthorn, izayoibara, ezokogi, jujube, perilla, lauren, saishin, koganebana, kihada, mulberry, button, peonies, chimpi, mukuroji, choji, lily, soybean In order to improve rough skin, a ceramide synthesis promoter characterized by containing one or two or more selected from the extract of white jellyfish is usually administered systemically or locally for internal or external use. The The dose varies depending on age, body weight, symptoms, therapeutic effect, administration method, treatment time, etc., but is usually 1 mg to 1 g per adult, preferably 20 mg to 200 mg once to several times a day. Is done. Since the dosage varies depending on various conditions, an amount smaller than the above-mentioned administration range may be sufficient, or it may be necessary to administer beyond the range.

  As the ceramide synthesis promoter for oral administration of the present invention, it can be used for any of foods, quasi drugs and pharmaceuticals, tablets, pills, powders, granules, capsules, emulsions, solutions Agents, suspensions, syrups, elixirs and the like. The above extract may be used as it is, and the excipient, extender, binder, wetting agent, disintegrant, surfactant, lubricant, dispersant, buffer, as long as the effect of the extract is not impaired. Agents, fragrances, preservatives, solubilizers, solvents and the like can be used. Specifically, lactose, sucrose, sorbit, mannitol, starch, precipitated calcium carbonate, heavy magnesium oxide, talc, calcium stearate, magnesium stearate, cellulose or derivatives thereof, amylopectin, polyvinyl alcohol, gelatin, surface activity Agents, water, physiological saline, ethanol, glycerin, propylene glycol, cacao butter, lauric fat, petrolatum, paraffin, higher alcohol and the like.

  The ceramide synthesis promoter for external use of the present invention can be used in any of cosmetics, quasi drugs and pharmaceuticals. Examples of the dosage form thereof include lotions, creams, emulsions, gels, and aerosols. , Essences, packs, cleaning agents, bath preparations, foundations, dusting powders, lipsticks, ointments, poultices, and the like. The above-mentioned extract may be used as it is, and the components used for the external preparation are within the range not impairing the effect of the extract, oils, waxes, hydrocarbons, fatty acids, alcohols, esters, interfaces Components such as activators, metal soaps, pH adjusters, preservatives, fragrances, moisturizers, powders, ultraviolet absorbers, thickeners, dyes, antioxidants, whitening agents, chelating agents, and the like can also be blended.

  The amount of the extract used in the present invention is 0.0001% by weight or more, preferably 0.001 to 50% by weight, in terms of solid matter, based on the ceramide synthesis accelerator of the present invention. If it is less than 0.0001% by weight, a sufficient effect is hardly expected. When it exceeds 50% by weight, the effect is hardly increased and it is uneconomical. In addition, the addition method may be added in advance or during the production, and may be appropriately selected in consideration of workability.

  Rice, crumbs, apricots, honeysuckle, marsh, tencha, rafma, hawthorn, izayoibara, ezokogi, jujube, perilla, lauren, saishin, koganebana, kihada, mulberry, button, peonies, chimpi, mukuroji, choji, lily, soybean And the extract of white jellyfish was excellent in ceramide synthesis promoting effect. In addition, a skin external preparation containing one or more of these extracts was safe, rough skin, and excellent in improving dry skin.

  Next, in order to describe the present invention in detail, examples of producing the extract used in the present invention, formulation examples of the present invention, and experimental examples will be given as examples, but the present invention is not limited thereto. The part of the amount shown in the examples indicates part by weight, and% indicates% by weight.

Production Example 1 Hot water extract of rice 2 L of purified water was added to 100 g of rice bran, extracted at 95-100 ° C. for 2 hours, filtered, and the filtrate was concentrated and freeze-dried to obtain a hot water extract of rice. 3.5 g was obtained.

Production Example 2 Rice Ethanol Extract 2L of ethanol was added to 100 g of rice bran and extracted at room temperature for 7 days, followed by filtration. The filtrate was concentrated to dryness to obtain 3.7 g of rice ethanol extract.

Production Example 3 Hot Water Extract of Scratch 2 L of purified water was added to 100 g of a dried product of Scrap leaves and extracted at 95-100 ° C. for 2 hours, followed by filtration. 2.7 g of water extract was obtained.

Production Example 4 Ethanol extract of kudzu 2 L of ethanol was added to 100 g of kudzu root and extracted at room temperature for 7 days, followed by filtration. The filtrate was concentrated to dryness to obtain 4.2 g of kudzu ethanol extract.

Production Example 5 Extract of apricot 50% 1,3-butylene glycol aqueous solution To 20 g of dried apricot leaves, 200 mL of purified water and 200 mL of 1,3-butylene glycol were added, extracted at room temperature for 7 days, filtered, and apricot. 370 g of 50% 1,3-butylene glycol extract was obtained.

Production Example 6 Apricot ethanol extract 1 L of ethanol was added to 100 g of apricot kernel, extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to dryness to obtain 5.5 g of apricot ethanol extract.

Production Example 7 Hot water extract of honeysuckle 2 L of purified water was added to 100 g of Shinobi winter, extracted at 95-100 ° C. for 2 hours, filtered, the filtrate was concentrated, freeze-dried, and 2 hot water extract of honeysuckle was obtained. .9 g was obtained.

Production Example 8 Honeysuckle ethanol extract 1 L of ethanol was added to 100 g of gold and silver flowers, extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to dryness to obtain 5.8 g of honeysuckle ethanol extract.

Production Example 9 Hot Water Extract of Yukinoshita 800 ml of purified water was added to 25 g of a dried whole plant of Yukinoshita, extracted at 95-100 ° C. for 2 hours, filtered, and the filtrate was concentrated and freeze-dried. 3.2 g of hot water extract was obtained.

Production Example 10 Tencha Ethanol Extract 1 L of ethanol was added to 100 g of dried tencha plant, extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to dryness to obtain 4.7 g of tencha ethanol extract. Obtained.

Production Example 11 Raffa Ethanol Extract 1 L of ethanol was added to 100 g of dried Raffa leaves and stems, extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to dryness to obtain Raffa ethanol extract. 5 g was obtained.

Production Example 12 Hawthorn 50% 1,3-butylene glycol aqueous solution extract 20 ml of Yamashiko was added 200 mL of purified water and 200 mL of 1,3-butylene glycol, extracted for 7 days at room temperature, filtered, 330 g of 3-butylene glycol extract was obtained.

Production Example 13 Hot Water Extract of Isaiyobara Add 800 mL of purified water to 25 g of dried leaves and stems of Isaiyobara, extract at 95-100 ° C. for 2 hours, filter, concentrate the filtrate, freeze-dry, 3.0 g of a hot water extract was obtained.

Production Example 14 Isaiyobara Ethanol Extract 1 L of ethanol was added to 100 g of pear and extracted at room temperature for 7 days, followed by filtration. The filtrate was concentrated to dryness to obtain 6.7 g of Isaiyobara ethanol extract.

Production Example 15 Hot water extract of Ekokogi 800 mL of purified water was added to 20 g of dried dried Ezogi root, extracted at 95-100 ° C. for 2 hours, filtered, and the filtrate was concentrated, freeze-dried and heated 3.7g of water extract was obtained.

Production Example 16 Hot water extract of jujube 800 ml of purified water was added to 20 g of dried jujube leaves, extracted at 95-100 ° C. for 2 hours, filtered, and the filtrate was concentrated, freeze-dried, 3.3 g of water extract was obtained.

Production Example 17 Ethanol Extract of Jujube 1 L of ethanol was added to 100 g of jujube and extracted at room temperature for 7 days, followed by filtration. The filtrate was concentrated to dryness to obtain 5.9 g of jujube ethanol extract.

Production Example 18 Perilla ethanol extract 1L of ethanol was added to 50 g of dried perilla leaves, extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to dryness to obtain 9.8 g of perilla ethanol extract. It was.

Production Example 19 Hot Water Extract of Aureen 2 L of purified water was added to 100 g of Yellow Ren, extracted at 95-100 ° C. for 2 hours, filtered, and the filtrate was concentrated and lyophilized to obtain a hot water extract of Aureen. 4.5 g was obtained.

Production Example 20 Hot Water Extract of Saisin 2 L of purified water was added to 100 g of the leaves of Usuba Saisin, extracted at 95-100 ° C. for 2 hours, filtered, the filtrate was concentrated, freeze-dried, and hot water of Saisin 4.0 g of extract was obtained.

Production Example 21 Ethylene extract of saicin 1 L of ethanol was added to 50 g of fine spices, extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to dryness to obtain 7.2 g of ethanol extract of saicin.

Production Example 22 Extract of 50% 1,3-butylene glycol aqueous solution of Koganebana 200 mL of purified water and 200 mL of 1,3-butylene glycol were added to 20 g of dried leaves and stems of Koganebana, extracted for 7 days at room temperature, and then filtered. 320 g of 50% 1,3-butylene glycol extract of Koganebana was obtained.

Production Example 23 Hot Water Extract of Koganebana Add 2 L of purified water to 100 g of yellow rice, extract at 95-100 ° C. for 2 hours, filter, concentrate the filtrate, freeze-dry and extract hot water extract of Koganebana 4.1g was obtained.

Production Example 24 Yellow Hot Water Extract of Yellowfin Addition of 2 L of purified water to 100 g of dried yellowfin root, extracted at 95-100 ° C. for 2 hours, filtered, concentrated, lyophilized and freeze-dried. 3.9 g of water extract was obtained.

Production Example 25 Yellow extract of yellowfin 1 lL of ethanol was added to 100 g of jaundice and extracted at room temperature for 7 days, followed by filtration. The filtrate was concentrated to dryness to obtain 7.6 g of yellowfin ethanol extract.

Production Example 26 Mulberry Hot Water Extract 2 L of purified water was added to 100 g of dried maggot root, extracted at 95-100 ° C. for 2 hours, filtered, the filtrate was concentrated, lyophilized and mulberry heat 4.0 g of water extract was obtained.

Production Example 27 Mulberry ethanol extract 1 L of ethanol was added to 100 g of mulberry white skin, extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to dryness to obtain 5.1 g of mulberry ethanol extract.

Production Example 28 Hot Water Extract of Button 2 L of purified water was added to 100 g of dried button leaf, extracted at 95-100 ° C. for 2 hours, filtered, and the filtrate was concentrated and freeze-dried to heat the button. 3.5 g of water extract was obtained.

Production Example 29 Extract from 50% 1,3-butylene glycol aqueous solution of button Add 200 mL of purified water and 200 mL of 1,3-butylene glycol to 20 g of peony skin, extract at room temperature for 7 days, filter, and filter 50% 1 of button , 3-butylene glycol extract was obtained.

Production Example 30 Peonies hot water extract 2 L of purified water was added to 100 g of glaze, extracted at 95-100 ° C. for 2 hours, filtered, and the filtrate was concentrated and freeze-dried to obtain 3 peonies hot water extract. .9 g was obtained.

Production Example 31 Hot Water Extract of Chinpi Add 2 L of purified water to 100 g of dried leaves and stems of Citrus unshiu, extract at 95-100 ° C. for 2 hours, filter, concentrate the filtrate and freeze-dry it. 3.1 g of hot water extract of chimpi was obtained.

Production Example 32 Ethylic extract of Chinpi 1 L of ethanol was added to 100 g of skin and extracted at room temperature for 7 days, followed by filtration. The filtrate was concentrated to dryness to obtain 4.5 g of Chinpi ethanol extract.

Production Example 33 Ethanol Extract of Mukuroji 1 L of ethanol was added to 100 g of dried mukuroji peel and extracted at room temperature for 7 days, followed by filtration. The filtrate was concentrated to dryness to obtain 4.4 g of an extract of mukuroji. .

Production Example 34 Hot Water Extract of Clove Add 2 L of purified water to 100 g of clove, extract at 95-100 ° C. for 2 hours, filter, concentrate the filtrate and freeze-dry to obtain 5 hot water extract of clove. 0.2 g was obtained.

Production Example 35 Lily 50% 1,3-butylene glycol aqueous solution extract To 20 g of dried Madonna lily bulbs, 200 mL of purified water and 200 mL of 1,3-butylene glycol were added, extracted at room temperature for 7 days, filtered, 320 g of a lily 50% 1,3-butylene glycol extract was obtained.

Production Example 36 Ethanol Extract of Soybean 1 L of ethanol was added to 100 g of dried soybean leaves, extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to dryness to obtain 4.2 g of soybean ethanol extract. It was.

Production Example 37 Soybean hot water extract 2 L of purified water was added to 100 g of soybean seeds, extracted at 95-100 ° C. for 2 hours, filtered, and the filtrate was concentrated and freeze-dried to obtain a hot water extract of soybeans. 5.0g was obtained

Manufacture example 38 Hot water extract of white jellyfish 4 L of purified water was added to 20 g of dried fruit body of white jellyfish, extracted at 95-100 ° C. for 2 hours, filtered, and the filtrate was concentrated, lyophilized and dried. 1.5 g of a hot water extract was obtained.

Formulation Example 1 Cream 1
Formulation Formulation 1. Waste ethanol extract (Production Example 4) 1.0 part Squalane 5.5
3. Olive oil 3.0
4). Stearic acid 2.0
5. Beeswax 2.0
6). Octyldodecyl myristate 3.5
7). Polyoxyethylene cetyl ether (20E.O.) 3.0
8). Behenyl alcohol 1.5
9. Glycerol monostearate2.5
10. Fragrance 0.1
11.1,3-butylene glycol 8.5
12 Ethyl paraoxybenzoate 0.05
13. Methyl paraoxybenzoate 0.2
14 Purified water 67.15
[Manufacturing method] Components 2 to 9 are heated and dissolved and mixed, and kept at 70 ° C to obtain an oil phase. Ingredients 1 and 11 to 14 are dissolved by heating and mixed, and kept at 75 ° C. to obtain an aqueous phase. The aqueous phase is added to the oil phase to emulsify, and the mixture is cooled while stirring. The component 10 is added at 45 ° C, and further cooled to 30 ° C to obtain a product.

Formulation Example 2 Cream 2
Formulation Formulation 1. 1.0 parts of hot water extract of white jellyfish (Production Example 38) Perilla ethanol extract (Production Example 18) 1.0
3. Squalane 5.5
4). Olive oil 3.0
5. Stearic acid 2.0
6). Beeswax 2.0
7). Octyldodecyl myristate 3.5
8). Polyoxyethylene cetyl ether (20E.O.) 3.0
9. Behenyl alcohol 1.5
10. Glycerol monostearate2.5
11. Fragrance 0.1
12.1,3-Butylene glycol 8.5
13. Ethyl paraoxybenzoate 0.05
14 Methyl paraoxybenzoate 0.2
15. Purified water 66.15
[Manufacturing method] Components 3 to 10 are dissolved by heating and mixed, and kept at 70 ° C to obtain an oil phase. Ingredients 1 and 2 and ingredients 12-15 are dissolved by heating and mixed, and kept at 75 ° C. to form an aqueous phase. The aqueous phase is added to the oil phase to emulsify, and the mixture is cooled while stirring.

Formulation Example 3 Cream 3
In Formulation Example 1, cream 3 was obtained by replacing the ethanol extract of kudzu with the hot water extract of auren (Production Example 19).

Comparative Example 1 Conventional Cream A conventional cream was prepared by replacing the ethanol extract of kudzu with purified water in Formulation Example 1.

Formulation Example 4 Lotion 1
Formulation Formulation 1. Yellowfin ethanol extract (Production Example 25) 0.1 part 2.1,3-butylene glycol 8.0
3. Glycerin 2.0
4). Xanthan gum 0.02
5. Citric acid 0.01
6). Sodium citrate 0.1
7). Ethanol 5.0
8). Methyl paraoxybenzoate 0.1
9. Polyoxyethylene hydrogenated castor oil (40E.O.) 0.1
10. Fragrance 0.1
11. Purified water 84.47
[Manufacturing method] Components 2-6 and 11 and components 1 and 7-10 are uniformly dissolved, mixed and filtered to obtain a product.

Formulation Example 5 Lotion 2
In Formulation Example 4, the lotion 2 was obtained by replacing yellowfin ethanol extract with apricot ethanol extract (Production Example 6).

Formulation Example 6 Lotion 3
In Formulation Example 4, lotion 3 was obtained by replacing yellowfin ethanol extract with honeysuckle ethanol extract (Production Example 8).

Formulation Example 7 Lotion 4
In Formulation Example 4, lotion 4 was obtained by replacing the ethanol extract of yellowfin with an ethanol extract of Rahuma (Production Example 11).

Formulation Example 8 Lotion 5
In Formulation Example 4, the lotion 5 was obtained by replacing the ethanol extract of yellowfin with an ethanol extract of Izayoi Rose (Production Example 14).

Formulation Example 9 Lotion 6
In Formulation Example 4, lotion 6 was obtained by replacing the ethanol extract of yellowfin with an ethanol extract of Saicin (Production Example 21).

Formulation Example 10 Lotion 7
In Formulation Example 4, the lotion 7 was obtained by replacing the yellow extract of yellowfin with an ethanol extract of soybean (Production Example 36).

Comparative Example 2 Conventional Lotion A conventional lotion was obtained by replacing the ethanol extract of yellowfin with purified water in Formulation Example 4.

Formulation Example 11 Latex 1
Formulation Formulation 1. Apricot ethanol extract (Production Example 6) 0.5 part
2. Squalane 5.0
3. Olive oil 5.0
4). Jojoba oil 5.0
5. Cetanol 1.5
6). Glycerol monostearate 2.0
7). Polyoxyethylene cetyl ether (20E.O.) 3.0
8). Polyoxyethylene sorbitan monooleate (20E.O.) 2.0
9. Fragrance
0.1
10. Propylene glycol
1.0
11. Glycerin
2.0
12 Methyl paraoxybenzoate
0.2
13. Purified water 72.7
[Manufacturing method] Components 2 to 8 are dissolved by heating and mixed, and kept at 70 ° C to obtain an oil phase. Ingredients 1 and 10-13 are dissolved by heating and mixed, and kept at 75 ° C. to form an aqueous phase. The aqueous phase is added to the oil phase to emulsify, and the mixture is cooled while stirring.

Formulation Example 12 Emulsion 2
In Prescription Example 11, 0.5 parts of apricot ethanol extract was replaced with 0.25 parts of honeysuckle ethanol extract (Production Example 8) and 0.25 parts of jujube ethanol extract (Production Example 17). 2.

Formulation Example 13 Gel agent Formulation Formulation amount 1. 1. Ethanol extract of saicin (Production Example 21) 1.0 part Ethanol 5.0
3. Methyl paraoxybenzoate 0.1
4). Polyoxyethylene hydrogenated castor oil (60 EO) 0.1
5. Fragrance 0.05
6.1,3-Butylene glycol 5.0
7). Glycerin 5.0
8). Xanthan gum 0.1
9. Carboxyvinyl polymer 0.2
10. Potassium hydroxide 0.2
11. Purified water 83.25
[Manufacturing method] Components 2 to 5 and components 1 and 6 to 11 are uniformly dissolved and mixed to obtain a product.

Formulation Example 14 Pack Formulation Formulation 1. Rice ethanol extract (Production Example 2) 0.1 part Mulberry ethanol extract (Production Example 27) 0.1
3. Polyvinyl alcohol 12.0
4). Ethanol 5.0
5.1,3-Butylene glycol 8.0
6). Methyl paraoxybenzoate 0.2
7). Polyoxyethylene hydrogenated castor oil (20 EO) 0.5
8). Citric acid 0.1
9. Sodium citrate 0.3
10. Fragrance 0.05
11. Purified water 73.65
[Production Method] Components 1 to 11 are uniformly dissolved to obtain a product.

Formulation Example 15 Foundation Formulation Formulation amount 1. Tencha ethanol extract (Production Example 10) 0.5 part Isaiyoibara ethanol extract (Production Example 14) 0.5
3. Stearic acid 2.4
4). Polyoxyethylene sorbitan monostearate (20EO) 1.0
5. Polyoxyethylene cetyl ether (20E.O.) 2.0
6). Cetanol 1.0
7). Liquid lanolin 2.0
8). Liquid paraffin 3.0
9. Isopropyl myristate 6.5
10. Sodium carboxymethylcellulose 0.1
11. Bentonite 0.5
12 Propylene glycol 4.0
13. Triethanolamine 1.1
14 Methyl paraoxybenzoate 0.2
15. Titanium dioxide 8.0
16. Talc 4.0
17. Bengala 1.0
18. Yellow iron oxide 2.0
19. Fragrance 0.1
20. Purified water 60.1
[Manufacturing method] Components 3 to 8 are dissolved by heating and kept at 80 ° C to form an oil phase. Swell component 10 well in component 20, then add components 1, 2 and 12-14 and mix uniformly. To this, components 15 to 18 pulverized and mixed with a pulverizer are added, and the mixture is stirred with a homomixer and kept at 75 ° C. to obtain an aqueous phase. The oily phase is added to the aqueous phase with stirring, cooled, and component 19 is added at 45 ° C, and cooled to 30 ° C with stirring to give a product.

Formulation Example 16 Ointment Formulation Formulation 1. Raffma ethanol extract (Production Example 11) 0.01 part 50% 1,3-butylene glycol extract of button (Production Example 29) 0.5
3. Polyoxyethylene cetyl ether (30E.O.) 2.0
4). Glycerol monostearate 10.0
5. Liquid paraffin 5.0
6). Cetanol 6.0
7). Methyl paraoxybenzoate 0.1
8). Propylene glycol 10.0
9. Purified water 66.39
[Production Method] Components 3 to 6 are dissolved by heating and mixed, and kept at 70 ° C. to obtain an oil phase. Ingredients 1, 2 and 7-9 are dissolved by heating and mixed, and kept at 75 ° C. to form an aqueous phase. The aqueous phase is added to the oil phase to emulsify, and the mixture is cooled to 30 ° C. with stirring to obtain a product.

Formulation Example 17 Beverage Formulation Formulation 1. 1. Hot water extract of Koganebana (Production Example 23) 1.0 part Stevia 0.05
3. Malic acid 5.0
4). Fragrance 0.1
5. Water 93.85
[Production Method] Components 2 and 3 are dissolved in a small amount of water. Components 1 and 4, 5 are then added and mixed.

Formulation Example 18 Tablet Formulation Formulation 1. Mukuroji ethanol extract (Production Example 33) 10.0 parts Corn starch 10.0
3. Purified white sugar 20.0
4). Carboxymethylcellulose 10.0
5. Microcrystalline cellulose 35.0
6). Polyvinylpyrrolidone 5.0
7). Talc 10.0
[Production Method] Components 1 to 5 were mixed, and then an aqueous solution of Component 6 was added as a binder and granulated by a conventional method. This was mixed with ingredient 7 as a lubricant and then tableted into 100 mg tablets.

  Next, experimental examples will be given to explain the effects of the present invention in detail.

Experimental Example 1 Ceramide Synthesis Promotion Test 1 × 10 ⁵ Pam212 cells, a mouse keratinocyte-derived cell line, were seeded in a 6 cm dish and cultured for 4 days. Thereafter, the sample was added to a final concentration of 10 μg / mL as a solid, and the culture was further continued for 24 hours. Next, the cells were washed three times with PBS (−), collected by a rubber policeman, and lyophilized. Lipids were extracted from cells with 1 mL of chloroform: methanol (2: 1), and the amount of ceramide in the cells was determined by the fluorescence method of Kisic et al. (Kisic A. and Rapport MM, Journal of Lipid Research, 15, 179-180. (1974)). That is, the lipid was hydrolyzed with 0.15 mL of 3N hydrochloric acid at 100 ° C. for 2 hours, dried in a desiccator, removed with hydrochloric acid, and then added with 2 mL of ethyl acetate and 1.25 mL of 0.1 M acetate buffer (pH 3.7). Stir well. Next, 0.25 mL of a fluorescamine solution (fluorescamine 7 mg / 25 mL acetone) was added, stirred well, then centrifuged, and the fluorescence intensity of the ethyl acetate layer was measured at Ex 410 nm and Em 490 nm. The amount of ceramide synthesis with no sample added was defined as the control, and the value of the amount of ceramide synthesized when the sample was added when the control was 100 was defined as the ceramide synthesis acceleration rate.

  The results of these experiments are shown in Table 1. As a result, rice, kudzu, apricot, honeysuckle, snow flakes, tencha, rafma, hawthorn, izayoi rose, elephant kogi, jujube, perilla, auren, saishin, koganebana, kihada, mulberry, button, peonies, chimpi, mukuroji, clove, clove, lily, soybean And the extract of white jellyfish showed an excellent ceramide synthesis promoting effect compared with the control.

  Rice, Kudzu, Apricot, Honeysuckle, Snowflake, Tencha, Rafuma, Hawthorn, Izayoi Rose, Ezocogi, Jujube, Perilla, Ouren, Saishin, Koganebana, Yellowfin, Mulberry, Button, Peonies, Chimpi, Mukuroji, Clove, Lily, Soybean When a mixture of two or more selected from the extracts was subjected to the same ceramide synthesis promotion test, it showed an excellent ceramide synthesis promotion effect.

Experiment 2 Use test 1
Using the skin lotions of Formulation Examples 4 to 10 and the conventional skin lotion of Comparative Example 2, a 1 month use test was conducted on 30 women (18 to 50 years old) who suffer from rough skin and dry skin. After use, the improvement effect of rough skin and dry skin was determined by questionnaire.

  The test results are shown in Table 2. As a result, the lotion obtained in Formulation Examples 4 to 10 was superior to the conventional lotion obtained in Comparative Example 2 in the effect of preventing and improving rough skin and dry skin. During the test period, there was no skin problem and there was no problem with safety.

  When other use lotions containing one or more selected from the extracts shown in Production Example 1 to Production Example 38 were used, they were safe and had excellent rough skin and dry skin. showed that.

Experiment 3 Use test 2
Using the creams of Formulation Examples 1 to 3 and the conventional cream of Comparative Example 1, a use test for 2 months was conducted on 30 women (18 to 50 years old) who suffer from rough skin and dry skin. After use, the improvement effect of rough skin and dry skin was determined by questionnaire.

  The test results are shown in Table 3. As a result, the creams obtained in Formulation Examples 1 to 3 were superior to the conventional cream obtained in Comparative Example 1 in the effect of preventing and improving rough skin and dry skin. During the test period, there was no skin problem and there was no problem with safety. There was also no problem with the deterioration of the prescription ingredients.

  When a usage test was conducted on Formulation Examples 11 to 18 as well, it was safe and exhibited excellent rough skin and a preventive and improving action on dry skin.

Rice, crumbs, apricots, honeysuckle, marsh, tencha, rafma, hawthorn, izayoibara, ezokogi, jujube, perilla, lauren, saishin, koganebana, kihada, mulberry, button, peonies, chimpi, mukuroji, choji, lily, soybean One or two or more selected from the extract of white jellyfish can be used as an excellent ceramide synthesis accelerator. In addition, it is possible to provide a composition that is safe, rough, and excellent in improving dry skin.

Claims (2)

Rice, Kudzu, Apricot, Honeysuckle, Snowflake, Tencha, Rafuma, Hawthorn, Izayoi Rose, Ezocogi, Jujube, Perilla, Ouren, Saishin, Koganebana, Yellowfin, Mulberry, Button, Peonies, Chimpi, Mukuroji, Clove, Lily, Soybean A ceramide synthesis promoter characterized by containing one or more selected from extracts. A composition excellent in the effect of preventing or improving rough skin and dry skin, comprising the ceramide synthesis accelerator according to claim 1.