JP2005532799A5 - - Google Patents
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- JP2005532799A5 JP2005532799A5 JP2004517153A JP2004517153A JP2005532799A5 JP 2005532799 A5 JP2005532799 A5 JP 2005532799A5 JP 2004517153 A JP2004517153 A JP 2004517153A JP 2004517153 A JP2004517153 A JP 2004517153A JP 2005532799 A5 JP2005532799 A5 JP 2005532799A5
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- magnetic
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- 239000000126 substance Substances 0.000 claims description 79
- 239000006249 magnetic particle Substances 0.000 claims description 78
- 239000000696 magnetic material Substances 0.000 claims description 27
- 239000002245 particle Substances 0.000 claims description 23
- 239000011159 matrix material Substances 0.000 claims description 21
- 230000005291 magnetic Effects 0.000 claims description 16
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 16
- 230000027455 binding Effects 0.000 claims description 14
- 239000007791 liquid phase Substances 0.000 claims description 13
- 238000006116 polymerization reaction Methods 0.000 claims description 12
- 239000000839 emulsion Substances 0.000 claims description 11
- 229920000642 polymer Polymers 0.000 claims description 11
- 125000000524 functional group Chemical group 0.000 claims description 10
- 229910052751 metal Inorganic materials 0.000 claims description 10
- 239000002184 metal Substances 0.000 claims description 10
- 239000000178 monomer Substances 0.000 claims description 10
- 108020004707 nucleic acids Proteins 0.000 claims description 8
- 150000007523 nucleic acids Chemical class 0.000 claims description 8
- 108090000623 proteins and genes Proteins 0.000 claims description 8
- 102000004169 proteins and genes Human genes 0.000 claims description 8
- 239000000377 silicon dioxide Substances 0.000 claims description 8
- 108091007650 binding proteins Proteins 0.000 claims description 6
- 102000024070 binding proteins Human genes 0.000 claims description 6
- 239000002902 ferrimagnetic material Substances 0.000 claims description 6
- 229910000460 iron oxide Inorganic materials 0.000 claims description 6
- 239000012071 phase Substances 0.000 claims description 6
- UQSXHKLRYXJYBZ-UHFFFAOYSA-N iron oxide Chemical compound [Fe]=O UQSXHKLRYXJYBZ-UHFFFAOYSA-N 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 5
- 244000005700 microbiome Species 0.000 claims description 5
- SZVJSHCCFOBDDC-UHFFFAOYSA-N Iron(II,III) oxide Chemical compound O=[Fe]O[Fe]O[Fe]=O SZVJSHCCFOBDDC-UHFFFAOYSA-N 0.000 claims description 4
- 102000004965 antibodies Human genes 0.000 claims description 4
- 108090001123 antibodies Proteins 0.000 claims description 4
- 238000010494 dissociation reaction Methods 0.000 claims description 4
- 230000005593 dissociations Effects 0.000 claims description 4
- 239000003446 ligand Substances 0.000 claims description 4
- PXHVJJICTQNCMI-UHFFFAOYSA-N nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 claims description 4
- 150000002894 organic compounds Chemical class 0.000 claims description 4
- 108090001008 Avidin Proteins 0.000 claims description 3
- 230000005293 ferrimagnetic Effects 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 229920000272 Oligonucleotide Polymers 0.000 claims description 2
- 241000700605 Viruses Species 0.000 claims description 2
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 2
- 229960002685 biotin Drugs 0.000 claims description 2
- 235000020958 biotin Nutrition 0.000 claims description 2
- 239000011616 biotin Substances 0.000 claims description 2
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 claims description 2
- 229910052793 cadmium Inorganic materials 0.000 claims description 2
- 239000002738 chelating agent Substances 0.000 claims description 2
- 229910052804 chromium Inorganic materials 0.000 claims description 2
- VYZAMTAEIAYCRO-UHFFFAOYSA-N chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 claims description 2
- 239000011651 chromium Substances 0.000 claims description 2
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 claims description 2
- 229910052803 cobalt Inorganic materials 0.000 claims description 2
- 239000010941 cobalt Substances 0.000 claims description 2
- 238000006482 condensation reaction Methods 0.000 claims description 2
- 239000000356 contaminant Substances 0.000 claims description 2
- 229910052802 copper Inorganic materials 0.000 claims description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 2
- 239000010949 copper Substances 0.000 claims description 2
- FYYHWMGAXLPEAU-UHFFFAOYSA-N magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims description 2
- 229910052749 magnesium Inorganic materials 0.000 claims description 2
- 239000011777 magnesium Substances 0.000 claims description 2
- PWHULOQIROXLJO-UHFFFAOYSA-N manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 claims description 2
- 229910052748 manganese Inorganic materials 0.000 claims description 2
- 239000011572 manganese Substances 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- 230000000813 microbial Effects 0.000 claims description 2
- 229910052759 nickel Inorganic materials 0.000 claims description 2
- 239000007764 o/w emulsion Substances 0.000 claims description 2
- 238000007348 radical reaction Methods 0.000 claims description 2
- 238000000527 sonication Methods 0.000 claims description 2
- 229910052723 transition metal Inorganic materials 0.000 claims description 2
- 150000003624 transition metals Chemical class 0.000 claims description 2
- 229910052720 vanadium Inorganic materials 0.000 claims description 2
- LEONUFNNVUYDNQ-UHFFFAOYSA-N vanadium(0) Chemical compound [V] LEONUFNNVUYDNQ-UHFFFAOYSA-N 0.000 claims description 2
- HCHKCACWOHOZIP-UHFFFAOYSA-N zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 2
- 229910052725 zinc Inorganic materials 0.000 claims description 2
- 239000011701 zinc Substances 0.000 claims description 2
- 230000002209 hydrophobic Effects 0.000 claims 3
- 229910044991 metal oxide Inorganic materials 0.000 claims 3
- 150000004706 metal oxides Chemical class 0.000 claims 3
- 230000002085 persistent Effects 0.000 claims 3
- 229920000620 organic polymer Polymers 0.000 claims 2
- CWYNVVGOOAEACU-UHFFFAOYSA-N fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 claims 1
- 239000003302 ferromagnetic material Substances 0.000 claims 1
- 239000007762 w/o emulsion Substances 0.000 claims 1
- 235000013980 iron oxide Nutrition 0.000 description 5
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 241000219430 Betula pendula Species 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- VBMVTYDPPZVILR-UHFFFAOYSA-N iron(2+);oxygen(2-) Chemical class [O-2].[Fe+2] VBMVTYDPPZVILR-UHFFFAOYSA-N 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
Description
【特許請求の範囲】
【請求項１】
磁性材料およびマトリクス材料を含み、前記磁性材料は磁場への曝露後に磁場の不存在下において液相で懸濁した場合に粒子が凝集塊を形成する程度に磁気の残留性があり、かつ前記マトリクス材料は液相の存在下で粒子の解離を促進する官能基を含む表面を有する、標的物質を結合することができる磁性粒子であって、前記磁性材料は鉄酸化物を含むフェリ磁性材料であり、前記マトリクス材料はシリカベースのポリマーを含み、及び前記官能基は親水性である、磁性粒子。
【請求項２】
鉄酸化物を含むフェリ磁性材料が、任意にその第一鉄のすべてまたは一部が、カドミウム、クロム、コバルト、銅、マグネシウム、マンガン、ニッケル、バナジウム、および／または亜鉛から選択された二価遷移金属で置換されている、請求項１に記載の磁性粒子。
【請求項３】
鉄酸化物を含むフェリ磁性材料がフェリ磁性磁鉄鉱を含む、請求項１に記載の磁性粒子。
【請求項４】
長さまたは直径が、０．１ないし５０００μｍの範囲内である、前記の請求項のいずれかに記載の磁性粒子。
【請求項５】
実質的に球形である、前記の請求項のいずれかに記載の磁性粒子。
【請求項６】
マトリクス材料が標的物質を結合するための親和性物質をさらに含む、前記の請求項のいずれかに記載の磁性粒子。
【請求項７】
標的物質が核酸である、前記の請求項のいずれかに記載の磁性粒子。
【請求項８】
親和性物質が、細胞、タンパク質、ウイルス、またはプリオンを結合することができる、請求項６に記載の磁性粒子。
【請求項９】
親和性物質が、抗体、結合タンパク質、抗体または結合タンパク質の断片、またはリガンドを含む、請求項８に記載の磁性粒子。
【請求項１０】
親和性物質が、ビオチニル化された標的物質への結合のためのアビジンを含む結合タンパク質を含むか、または親和性物質がビオチンを含み、かつ標的物質がアビジン化された、請求項９に記載の磁性粒子。
【請求項１１】
親和性物質が、標的物質に特異的なオリゴヌクレオチドまたは金属キレートを含むリガンドを含む、請求項９に記載の磁性粒子。
【請求項１２】
細胞またはタンパク質が微生物起源である、請求項８から１１のいずれかに記載の磁性粒子。
【請求項１３】
標的物質が金属を含み、かつ親和性物質が金属に対するキレート剤を含む、請求項６に記載の磁性粒子。
【請求項１４】
磁化されていない磁性材料を提供すること、および磁性粒子を形成するようにマトリクス材料を提供することを含み、前記磁性材料は磁場への曝露後に磁場の不存在下において液相で懸濁した場合に粒子が凝集塊を形成する程度に磁気の残留性があり、かつ前記マトリクス材料は液相の存在下で粒子の解離を促進する官能基を含む表面を有する、標的物質を結合することができる磁性粒子の調製のための方法であって、前記磁性材料は鉄酸化物を含むフェリ磁性材料であり、前記マトリクス材料はシリカベースのポリマーを含み、及び前記官能基は親水性である、磁性粒子の調製のための方法。
【請求項１５】
マトリクス材料が予備成型されて提供され、磁性材料に添加される、請求項１４に記載の方法。
【請求項１６】
シリカベースのポリマーが、磁化されていない磁性材料の存在下でのモノマーの重合によって提供される、請求項１４に記載の方法。
【請求項１７】
重合の段階が逐次縮合および／またはラジカル反応を含む、請求項１６に記載の方法。
【請求項１８】
重合の段階がエマルジョン中で起こり、磁化されていない磁性材料がエマルジョンの不連続相に存在する、請求項１６から１７のいずれかに記載の方法。
【請求項１９】
重合の段階がエマルジョンの不連続相で起こる、請求項１８に記載の方法。
【請求項２０】
モノマーが重合の前にエマルジョンの連続相に存在する、請求項１８または請求項１９に記載の方法。
【請求項２１】エマルジョンが水中油型エマルジョンである、請求項２０に記載の方法。
【請求項２２】
重合の段階が溶液中で起こる、請求項１６から１７のいずれかに記載の方法。
【請求項２３】
磁性材料が、長さまたは直径が１００ないし３００ｎｍの範囲内である粒子を含む、請求項１４から２２のいずれかに記載の方法。
【請求項２４】
磁性粒子が請求項１から１３のいずれかで定義される通りである、請求項１４から２３のいずれかに記載の方法。
【請求項２５】
標的物質を、そのような標的物質を含む試料から分離するために用いられる、請求項１から１３のいずれかに記載の、または請求項１４から２４のいずれかに記載の方法によって入手可能な、磁性粒子。
【請求項２６】
細胞、微生物、またはタンパク質を含む標的物質を、そのような標的物質を含む試料から分離するために用いられる、請求項１から６、または８から１２のいずれかに記載の磁性粒子。
【請求項２７】
金属を含む標的物質を、そのような標的物質を含む試料から分離するために用いられる、請求項１から６、または１３のいずれかに記載の磁性粒子。
【請求項２８】
有機化合物を含む標的物質を、そのような標的物質を含む試料から分離するために用いられる、請求項１から６のいずれかに記載の磁性粒子。
【請求項２９】
核酸を含む標的物質を、そのような標的物質を含む試料から分離するために用いられる、請求項１から７のいずれかに記載の磁性粒子。
【請求項３０】
標的物質が試料から単離される、請求項２５から２９のいずれかに記載の磁性粒子。
【請求項３１】
標的物質が試料から除去される、請求項２５から２９のいずれかに記載の磁性粒子。
【請求項３２】
セルソーティング装置に用いられる、請求項１から６、または８から１２のいずれかに記載の磁性粒子。
【請求項３３】
（ａ）磁性材料およびマトリクス材料を含み、前記磁性材料は磁場への曝露後に磁場の不存在下において液相で懸濁した場合に粒子が凝集塊を形成する程度に磁気の残留性がある、標的物質を結合する磁性粒子であって、前記磁性材料は鉄酸化物を含むフェリ磁性材料であり、前記マトリクス材料はシリカベースのポリマーを含み、及び前記官能基は親水性である、磁性粒子を提供すること；
（ｂ）標的物質を含む試料を含む液相を提供すること；
（ｃ）標的物質を粒子に結合させるように試料を磁性粒子と共に分散し、磁性粒子を破壊に供して粒子を解離させること；および
（ｄ）磁場を粒子に加え、かつ粒子を液相から分離することによって、粒子を試料から単離すること
：を含む、標的物質を、標的物質を含む試料から分離するための方法であって、前記破壊はピペッティング、攪拌、ボルテックスおよび／または振盪、超音波処理またはＵＶ破壊から選ばれる機械的な破壊を含む、
標的物質を、標的物質を含む試料から分離するための方法。
【請求項３４】
磁性粒子が請求項１から１３のいずれかで定義される通りであるかまたは、請求項１４から２４のいずれかで定義される方法によって得られる、請求項３３に記載の方法。
【請求項３５】
磁性粒子が請求項１から６、または８から１２のいずれかで定義される通りであり、標的物質が細胞、微生物、またはタンパク質を含む、請求項３３から３４のいずれかに記載の方法。
【請求項３６】
磁性粒子は請求項１から６、または１３のいずれかで詳述した通りであり、標的物質が金属を含む、請求項３３から３４のいずれかに記載の方法。
【請求項３７】
磁性粒子は請求項１から６のいずれかで定義した通りであり、標的物質が有機化合物を含む、請求項３３から３４のいずれかに記載の方法。
【請求項３８】
磁性粒子は請求項１から７のいずれかで定義した通りであり、標的物質が核酸を含む、請求項３３から３４のいずれかに記載の方法。
【請求項３９】
試料が未分画の核酸を含む、請求項３８に記載の方法。
【請求項４０】
標的物質が試料から単離される、請求項３３から３９のいずれかに記載の方法。
【請求項４１】
標的物質が試料から除去される夾雑物である、請求項３３から３９のいずれかに記載の方法。
[Claims]
[Claim 1]
Includes a magnetic material and a matrix material, wherein the magnetic material is particles when suspended in the liquid phase is the remaining Tomesei magnetic enough to form clumps in the absence of a magnetic field after exposure to a magnetic field, and wherein The matrix material is a magnetic particle capable of binding a target substance having a surface containing a functional group that promotes dissociation of the particle in the presence of a liquid phase, and the magnetic material is a ferrimagnetic material containing iron oxide. Magnetic particles wherein the matrix material comprises a silica-based polymer and the functional groups are hydrophilic .
[Claim 2 ]
Iron oxides including ferrimagnetic material, all or a portion of any in its first iron, cadmium, chromium, cobalt, copper, magnesium, manganese, nickel, vanadium, and / or divalent selected from zinc The magnetic particle according to claim 1 , which is substituted with a transition metal.
[Claim 3 ]
Ferrimagnetic material containing iron oxide containing ferrimagnetic magnetite, magnetic particles according to claim 1.
[Claim 4 ]
Magnetic particle according to any of the preceding claims, wherein the length or diameter is in the range of 0.1 to 5000 µm.
[Claim 5 ]
Magnetic particle according to any of the preceding claims, which is substantially spherical.
[Claim 6 ]
Magnetic particle according to any of the preceding claims, wherein the matrix material further comprises an affinity substance for binding the target substance.
[Claim 7 ]
The magnetic particle according to claim 1, wherein the target substance is a nucleic acid.
[Claim 8 ]
The magnetic particle according to claim 6 , wherein the affinity substance is capable of binding a cell, a protein, a virus, or a prion.
[Claim 9 ]
The magnetic particle according to claim 8 , wherein the affinity substance includes an antibody, a binding protein, an antibody or a fragment of the binding protein, or a ligand.
[Claim 10 ]
Affinity substance comprises or binding proteins comprising avidin for binding to biotinylated target substance, or affinity substance comprises biotin, and the target substance is avidin reduction, according to claim 9 Magnetic particles.
11. The method of claim 11
The magnetic particle according to claim 9 , wherein the affinity substance includes a ligand containing an oligonucleotide or metal chelate specific to the target substance.
[Claim 12 ]
The magnetic particle according to any one of claims 8 to 11 , wherein the cell or protein is of microbial origin.
[Claim 13 ]
The magnetic particle according to claim 6 , wherein the target substance contains a metal and the affinity substance contains a chelating agent for the metal.
14.
Providing a non-magnetized magnetic material and providing a matrix material to form magnetic particles, wherein the magnetic material is suspended in a liquid phase in the absence of a magnetic field after exposure to the magnetic field the particles has magnetic residual Tomesei enough to form agglomerates, and said matrix material has a surface comprising functional groups that promote dissociation of the particles in the presence of a liquid phase, to bind the target substance A method for the preparation of magnetic particles , wherein the magnetic material is a ferrimagnetic material comprising iron oxide, the matrix material comprises a silica-based polymer, and the functional groups are hydrophilic. Method for the preparation of particles .
[Claim 15 ]
The method of claim 14 , wherein the matrix material is provided preformed and added to the magnetic material.
16.
Silica-based polymer is provided by the polymerization of monomers in the presence of a magnetic material not magnetized, the method according to claim 14.
[Claim 17 ]
The process according to claim 16 , wherein the stage of polymerization comprises sequential condensation and / or radical reaction.
[Claim 18 ]
18. A method according to any of claims 16 to 17 , wherein the polymerization step occurs in the emulsion and the unmagnetized magnetic material is present in the discontinuous phase of the emulsion.
[Claim 19 ]
19. A process according to claim 18 , wherein the polymerization stage occurs in the discontinuous phase of the emulsion.
[ 20 ]
20. A process according to claim 18 or claim 19 , wherein the monomer is present in the continuous phase of the emulsion prior to polymerization.
21. The method of claim 20 , wherein the emulsion is an oil-in-water emulsion.
[Claim 22 ]
18. A method according to any of claims 16 to 17 , wherein the polymerization step occurs in solution.
23.
23. A method according to any of claims 14 to 22 , wherein the magnetic material comprises particles having a length or diameter in the range of 100 to 300 nm.
24.
24. A method according to any of claims 14 to 23 , wherein the magnetic particles are as defined in any of claims 1 to 13 .
25.
A target substance is used to separate from a sample containing such target substance, obtainable by a method according to any of claims 1 to 13 or according to any of claims 14 to 24 , Magnetic particles.
26.
The magnetic particle according to any one of claims 1 to 6 or 8 to 12 , which is used for separating a target substance containing cells, microorganisms, or proteins from a sample containing such a target substance.
27.
A target substance containing a metal is used to separate from a sample containing such a target substance, magnetic particles according to any of claims 1 6 or 13,.
28.
The target material containing an organic compound, is used to separate from a sample containing such a target substance, magnetic particles according to any of claims 1 to 6.
29.
The magnetic particle according to any one of claims 1 to 7 , which is used for separating a target substance containing a nucleic acid from a sample containing such a target substance.
30.
The magnetic particle according to any one of claims 25 to 29 , wherein the target substance is isolated from the sample.
[Claim 31 ]
The magnetic particle according to any one of claims 25 to 29 , wherein the target substance is removed from the sample.
32.
The magnetic particles according to any one of claims 1 to 6 and 8 to 12 , which are used in a cell sorting apparatus.
33.
(A) comprise a magnetic material and a matrix material, wherein the magnetic material has a magnetic residual Tomesei to the extent that particles form aggregates when suspended in a liquid phase in the absence of a magnetic field after exposure to the magnetic field A magnetic particle that binds a target substance , wherein the magnetic material is a ferrimagnetic material including iron oxide, the matrix material includes a silica-based polymer, and the functional group is hydrophilic. Providing;
(B) providing a liquid phase containing a sample containing the target substance;
(C) Dispersing the sample with the magnetic particles to bind the target substance to the particles, subjecting the magnetic particles to break up to dissociate the particles; and (d) applying a magnetic field to the particles and separating the particles from the liquid phase. Isolating the particles from the sample by: separating the target substance from the sample containing the target substance , wherein the disruption comprises pipetting, stirring, vortexing and / or shaking, ultra Including mechanical destruction selected from sonication or UV destruction,
A method for separating a target substance from a sample containing the target substance .
34.
34. A method according to claim 33 , wherein the magnetic particles are as defined in any of claims 1 to 13 or obtained by a method as defined in any of claims 14 to 24 .
35.
35. A method according to any of claims 33 to 34 , wherein the magnetic particles are as defined in any of claims 1 to 6 or 8 to 12 , and the target substance comprises cells, microorganisms or proteins.
36.
35. A method according to any of claims 33 to 34 , wherein the magnetic particles are as detailed in any of claims 1 to 6 or 13 , and the target substance comprises a metal.
37.
35. A method according to any of claims 33 to 34 , wherein the magnetic particles are as defined in any of claims 1 to 6 and the target substance comprises an organic compound.
38.
35. A method according to any of claims 33 to 34 , wherein the magnetic particles are as defined in any of claims 1 to 7 and the target substance comprises a nucleic acid.
39.
40. The method of claim 38 , wherein the sample comprises unfractionated nucleic acid.
40.
40. A method according to any of claims 33 to 39 , wherein the target substance is isolated from the sample.
41.
40. The method according to any one of claims 33 to 39 , wherein the target substance is a contaminant to be removed from the sample.
試料を磁性粒子と共に分散する段階は、好ましくは粒子を解離させるために磁性粒子を破壊に供することを含む。破壊は機械的破壊、音波式破壊、またはＵＶ破壊を含む。機械的破壊は、粒子を解離させるようなピペッティング、攪拌、ボルテックスおよび／または振盪を含む。音波式破壊は、超音波法およびＵＶ破壊を含む。標的物質の粒子への結合を最大化するように、試料が磁性粒子と可能な限り完全に分散することが重要である。 Dispersing the sample with the magnetic particles preferably includes subjecting the magnetic particles to breakage to dissociate the particles. Destruction includes mechanical destruction, sonic destruction, or UV destruction. Mechanical disruption includes pipetting, agitation, vortexing and / or shaking to dissociate the particles. Sonic destruction includes ultrasonic methods and UV destruction. It is important that the sample is dispersed as completely as possible with the magnetic particles so as to maximize the binding of the target substance to the particles.
Claims (55)
（ｂ）標的物質を含む試料を含む液相を提供すること；
（ｃ）標的物質を粒子に結合させるように試料を磁性粒子と共に分散すること；および
（ｄ）磁場を粒子に加え、かつ粒子を液相から分離することによって、粒子を試料から単離すること
：を含む、標的物質を、標的物質を含む試料から分離するための方法。 (A) providing magnetic particles that bind a target substance, comprising a magnetic material and a matrix material, wherein the magnetic material is persistent upon exposure to a magnetic field;
(B) providing a liquid phase containing a sample containing the target substance;
(C) dispersing the sample with the magnetic particles so as to bind the target substance to the particles; and (d) isolating the particles from the sample by applying a magnetic field to the particles and separating the particles from the liquid phase. A method for separating a target substance from a sample containing the target substance.
The method according to any one of claims 45 to 53, wherein the target substance is a contaminant to be removed from the sample.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0215185.0A GB0215185D0 (en) | 2002-07-01 | 2002-07-01 | Binding a target substance |
| PCT/IB2003/002994 WO2004003231A2 (en) | 2002-07-01 | 2003-07-01 | Remanent magnetic paricles capable of binding a target substance, their production and uses thereof |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2005532799A JP2005532799A (en) | 2005-11-04 |
| JP2005532799A5 true JP2005532799A5 (en) | 2009-02-26 |
| JP4324101B2 JP4324101B2 (en) | 2009-09-02 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2004517153A Expired - Lifetime JP4324101B2 (en) | 2002-07-01 | 2003-07-01 | Binding of target substance |
Country Status (6)
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| US (3) | US20060188876A1 (en) |
| EP (1) | EP1520041B1 (en) |
| JP (1) | JP4324101B2 (en) |
| CA (1) | CA2490962C (en) |
| GB (1) | GB0215185D0 (en) |
| WO (1) | WO2004003231A2 (en) |
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2002
- 2002-07-01 GB GBGB0215185.0A patent/GB0215185D0/en not_active Ceased
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- 2003-07-01 JP JP2004517153A patent/JP4324101B2/en not_active Expired - Lifetime
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2012
- 2012-10-02 US US13/633,626 patent/US20130089909A1/en not_active Abandoned
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