JP2005239694A - Anti-stress composition - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To obtain an inexpensive anti-stress composition capable of effectively relaxing the symptoms of mind and body in the case under a stressed state and also safe and easily available. <P>SOLUTION: This anti-stress composition consists of materials containing tea polyphenols. Also, the anti-stress composition is used for relaxing the symptoms of mind and body in the treatment of renal diseases. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

  The present invention relates to an anti-stress composition comprising a material containing tea polyphenols.

  Stress refers to strain that occurs in the mind and body when various external stimuli work as a burden. Stress-causing elements (stressors) include physicochemical things such as cold, noise, chemicals, biological things such as hunger, infection, overwork, lack of sleep, social tension, anxiety, fear, excitement, etc. Typical ones. In today's complex society, people are exposed to various types of stressors and it is difficult to avoid them in daily life.

  When the living body is continuously exposed to stressors, that is, under continuous stress, heart rate or blood pressure increases, sleeplessness, fatigue, joint pain, headache, stiff shoulders, eye strain, loss of appetite, constipation, etc. Symptoms appear. Further exposure to stressors affects various organs throughout the body, resulting in severe psychosomatic disorders, peptic ulcers, heart disease, cerebrovascular disorders, hypertension, hyperlipidemia, and the like.

  Currently, drugs used for the purpose of alleviating psychosomatic symptoms in the state of stress or for the purpose of disease prevention are mainly benzodiazepine anxiolytics or sleeping drugs. Although these are relatively safe, they are addictive and have side effects, so it is not preferable to use them frequently. In addition, if the use is interrupted after continuous use, a recoil phenomenon may occur and the symptoms may even worsen. Therefore, it is difficult to say that it is an ideal anti-stress agent.

Moreover, as an anti-stress composition using a food material or a food additive, β-carotene (for example, see Patent Document 1), L-theanine (for example, see Patent Document 2), nucleic acid (for example, see Patent Document 3) ), Imidazole compounds (for example, see Patent Document 4), astaxanthin (for example, see Patent Document 5), sour milk (for example, see Patent Document 6), gluten (for example, see Patent Document 7), plants belonging to the family Oysteraceae A solvent extract (for example, see Patent Document 8), a royal jelly (for example, see Patent Document 9), and the like are known.
JP-A-6-65068 Japanese Patent Laid-Open No. 6-100442 JP 7-215879 A Japanese Patent Laid-Open No. 9-20660 JP-A-9-124470 Japanese Patent Laid-Open No. 10-45610 Japanese Patent Laid-Open No. 11-49697 JP 2000-136143 A JP 2001-213792 A

  However, the above composition is not suitable for actual use because the effect is not necessarily sufficiently exhibited, the effect is not stable, the cost is high, and the availability is problematic. Moreover, since this composition temporarily relieves slight stress, it was difficult to say that it was an ideal anti-stress agent.

  Accordingly, an object of the present invention is to provide an inexpensive anti-stress composition that can effectively relieve psychosomatic symptoms in a stress state and is safe and easily available.

That is, the present invention
(1) an anti-stress composition comprising a material containing tea polyphenols,
(2) The anti-stress composition according to (1) above for alleviating psychosomatic symptoms in the treatment of kidney disease,
(3) The antistress composition according to (1) or (2), which is a food or a medicine, and (4) the antistress composition according to (3), which is either a tablet or a capsule.

  According to the present invention, it is possible to provide an inexpensive anti-stress composition that can effectively relieve mental and physical reactions when in a stress state and is safe and highly available.

  The anti-stress composition of this invention contains the material containing tea polyphenols.

  Examples of the tea polyphenols in the present invention include flavonoids such as flavones, flavonols, flavanones, isoflavones, anthocyanins, flavanols, other non-flavonoids, derivatives and polymers thereof.

  The tea in the present invention is not particularly limited, but botanically includes green tea that is non-fermented tea produced from leaves of camellia, oolong tea that is semi-fermented tea, black tea that is fermented tea, and the like. Among them, it is preferable to use green tea which is non-fermented tea from the viewpoint of active ingredients.

  The material containing tea polyphenols used in the present invention is not particularly limited as long as it contains tea polyphenols. For example, tea leaves, tea extracts, tea polyphenols themselves are isolated and purified, or What was synthesize | combined etc. is mentioned, These can use 1 type (s) or 2 or more types.

  Examples of tea leaves include green tea leaves, oolong tea leaves, black tea leaves, and the like. When using tea leaves, the tea leaves may be used as they are, or may be used after being pulverized.

  Examples of the tea extract include tea extracts, tea leaves or tea leaves obtained by extracting tea leaves or ground tea leaves with water, hot water, monohydric alcohols such as methanol, ethanol, propanol or polyhydric alcohols such as glycerin. It is obtained by fractionating the ground tea leaves by adding a solvent such as ethyl acetate or acetone to a fraction extracted using a monohydric alcohol such as water, hot water, methanol, ethanol or propanol or a polyhydric alcohol such as glycerin. Tea extract and the like.

  Preferably, from the viewpoint of the active ingredient, ethyl acetate or acetone is added to a fraction obtained by extracting tea leaves or crushed tea leaves with a monohydric alcohol such as water, hot water, methanol, ethanol or propanol or a polyhydric alcohol such as glycerin. A tea extract obtained by fractionation by adding a solvent such as, more preferably, tea from which caffeine has been removed from the tea extract by column chromatography from the viewpoint of improving the flavor and further improving the anti-stress effect. It is an extract.

  Examples of tea polyphenols include the same as those described above. The isolation / purification or synthesis method may be a method known in the art.

  The content of tea polyphenols in the material is not particularly limited, but from the viewpoint of further improving the anti-stress effect, it is preferably 10% by weight or more in terms of dry matter, more preferably 30% by weight or more, 70% by weight or more is particularly preferable. The content can be measured by the method described in the following examples.

  The content of tea polyphenols in the anti-stress composition of the present invention is preferably 0.01 to 100% by weight, more preferably 0.2 to 70% by weight.

In the anti-stress composition of the present invention, in addition to the material containing tea polyphenols, various nutritional components and the like may be strengthened within a range not inhibiting the desired effect of the present invention. Nutritional ingredients that can be strengthened include vitamins such as vitamin A, vitamin B ₁ , vitamin B ₂ , vitamin B ₆ , vitamin B ₁₂ , vitamin C, vitamin D, vitamin E, niacin (nicotinic acid), pantothenic acid, folic acid; Essential amino acids such as lysine, threonine and tryptophan; minerals such as calcium, magnesium, iron, zinc and copper; α-linolenic acid, EPA, DHA, evening primrose oil, octacosanol, casein phosphopeptide (CPP), casein calcium peptide ( CCP), water-soluble dietary fiber, insoluble dietary fiber, oligosaccharides and other substances that contribute to human health; other useful substances approved as foods and food additives, etc. One or more can be used.

  The anti-stress composition of the present invention is derived from natural ingredients such as tea that is drunk everyday, and has almost no problem in safety to the human body.

  The antistress composition of the present invention contains commercially available tea polyphenols such as sunphenon (manufactured by Taiyo Kagaku), theafuran (manufactured by ITO EN), sun woolon (manufactured by Suntory), polyphenon (manufactured by Tokyo Food Techno). Materials can also be used.

  The anti-stress composition of the present invention can effectively relieve mental and physical symptoms when in a stress state. More specifically, psychosomatic symptoms due to physical stress and mental stress can be alleviated.

  For example, as stressors in the treatment of diseases, there are rapid environmental changes, various restrictions, physical and chemical stressors such as drugs, anxiety about diseases, anxiety about side effects of drugs, anxiety due to changes in body image such as body shape and physical condition, hospitalization Social stressors such as delays in work and schooling due to anger and anxiety about friendship. These stressors put the patient in stress and cause various psychosomatic symptoms. Psychosomatic symptoms include increased heart rate or blood pressure, insomnia, fatigue, joint pain, headache, stiff shoulders, eye strain, loss of appetite, and constipation.

  For example, in the treatment of renal diseases, stressors such as diet restriction, pain at the time of puncture of a dialysis needle, and restraint over several hours during dialysis are known. These stressors put the patient in stress and manifest psychosomatic symptoms such as anxiety, insomnia, loss of appetite, constipation, headache, joint pain, skin roughness, and increased blood pressure. The anti-stress composition of the present invention can alleviate these mental and physical symptoms.

  Another stressor is smoking. Smoking causes symptoms such as an increase in the amount of reactive oxygen species in the blood due to stress on the smoker or the person around the smoker. It is known that when such a stress state continues for a long period of time, the risk of ischemic heart disease, stroke, lung cancer, etc. increases. In addition, the effects of passive smoking during pregnancy or breastfeeding on the fetus or child are also problematic. The anti-stress composition of the present invention can also alleviate this symptom.

  Moreover, as a composition of this invention, a foodstuff or a pharmaceutical is preferable from a viewpoint that it is suitable for daily use.

  The food in the present invention includes not only a food containing a material containing tea polyphenols but also a food additive containing a material containing tea polyphenols.

  Examples of foods included in the present invention include solid foods such as dried foods and supplements, and liquid foods such as soft drinks, mineral water, taste drinks, and alcoholic drinks. Examples of the solid food include, but are not limited to, kneaded products, processed soybean products, seasonings, mousses, jelly, frozen desserts, strawberries, chocolate, gum, crackers, cakes, breads and the like. The liquid food is not particularly limited, but teas such as green tea, oolong tea, black tea, herbal tea, concentrated fruit juice, concentrated reduced juice, straight juice, fruit mixed juice, fruit juice with fruit juice, beverage with fruit juice , Fruit / vegetable mixed juice, vegetable juice, carbonated drink, soft drink, milk drink, sake, beer, wine, cocktail, shochu, whiskey, etc.

  Moreover, as a pharmaceutical included by this invention, if the material containing tea polyphenols is included, it will not specifically limit. For example, the form may be any of a solution, suspension, powder, solid molding, etc., and the dosage form is a tablet, capsule, powder, granule, drink, injection, patch, Suppositories, inhalants and the like can be mentioned.

  The production method of the composition of the present invention is not particularly limited, for example, a production method in which a material containing tea polyphenols and other raw materials are powder-mixed, a material containing tea polyphenols in a solvent, and other materials A general method for producing foods or pharmaceuticals can be applied, such as a method for dissolving raw materials into a mixed solution, a method for freeze-drying the mixed solution, or a method for spray drying.

  For example, a material containing tea polyphenols is mixed with an existing food by a conventional method so that the content of tea polyphenols in the food of the present invention after production is preferably within the above-mentioned preferred content range. The food of the invention can be manufactured. In addition, the material containing tea polyphenols, for example, organic or inorganic carriers, excipients, binders, stabilizers and the like suitable for known oral administration, preferably as described above for food production Thus, the pharmaceutical product of the present invention can be produced by mixing materials containing tea polyphenols by a conventional method so that the content range of tea polyphenols is within the range.

  The dose of the composition of the present invention for obtaining the desired effect of the present invention is usually preferably 50 to 2000 mg / day, more preferably 200 to 1000 mg / day as tea polyphenols. However, since there are individual differences (such as the type and degree of stress state, age, etc.), the dose in the present invention is not limited to such a range, and individual doses can be obtained so as to obtain the desired effect of the present invention. Specifically, the dose may be set as appropriate.

  The administration method of the composition of the present invention is not particularly limited, but is preferably oral administration, intravenous administration, rectal administration, more preferably oral administration, and may be administered within the above-mentioned preferred range.

  The present invention will be described below with reference to examples and test examples, but the scope of the present invention is not limited thereto.

Example 1
1 L of deionized water was added to 100 g of green tea leaves cut to 30 Mesh Pass or less, and tea polyphenols were extracted at 80 ° C. for 1 hour. After cooling to room temperature, the extracted residue was removed with filter paper, the filtrate was concentrated under reduced pressure, and freeze-dried to obtain 20 g of green tea extract (Invention product A). The content of tea polyphenols in the obtained green tea extract was measured by a tannin colorimetric method and found to be 35% by weight.

Example 2
16 g of oolong tea extract (present invention product B) was obtained in the same manner as in Example 1 except that oolong tea leaves were used and extraction was performed at 40 ° C. The content of tea polyphenols in the obtained oolong tea extract was measured in the same manner as in Example 1. As a result, it was 17% by weight.

Example 3
22 g of green tea extract (present invention product C) was obtained in the same manner as in Example 1 except that 50% aqueous ethanol was used as an extraction solvent and extraction was performed at 60 ° C. The content of tea polyphenols in the obtained oolong tea extract was measured in the same manner as in Example 1. As a result, it was 33% by weight.

Example 4
1 L of deionized water was added to 100 g of green tea leaves cut to 30 Mesh Pass or less, and extraction was performed at 80 ° C. for 1 hour. After cooling to room temperature, the extracted residue was removed with filter paper, and the filtrate was concentrated under reduced pressure. The filtrate concentrated under reduced pressure was partitioned by adding 1 L of ethyl acetate to obtain an ethyl acetate fraction. This fraction was concentrated under reduced pressure to distill off ethyl acetate, and then dissolved in deionized water and lyophilized to obtain 7 g of green tea extract (present invention D). The content of tea polyphenols in the obtained green tea extract was measured in the same manner as in Example 1. As a result, it was 82% by weight.

Example 5
1 L of deionized water was added to 100 g of green tea leaves cut to 30 Mesh Pass or less, and extraction was performed at 80 ° C. for 1 hour. After cooling to room temperature, the extracted residue was removed with filter paper, and the filtrate was concentrated under reduced pressure. The filtrate concentrated under reduced pressure was partitioned by adding 1 L of ethyl acetate to obtain an ethyl acetate fraction. This fraction was concentrated under reduced pressure to distill off ethyl acetate and then packed into a column of cyclodextrin polymer (prepared by epichlorohydrin method) equilibrated with water. This fraction was eluted with water and then with ethanol, and the obtained fractions were collected, concentrated under reduced pressure, and lyophilized to obtain 6 g of decaffeinated green tea extract (present product E). The content of tea polyphenols in the obtained green tea extract was measured in the same manner as in Example 1. As a result, it was 85% by weight.

Example 6 Preparation of tablets containing tea polyphenols Tablets having the following composition of 250 mg per tablet were prepared according to a conventional method.
Green tea extract of Example 5 40.0% by weight
Lactose 33.5% by weight
Crystalline cellulose 20.4% by weight
Sucrose ester 3.1% by weight
Orange flavor 3.0% by weight

Example 7 Preparation of tea polyphenols-containing candy According to a conventional method, a granulated sugar aqueous solution was prepared by heating to 110 ° C. while dissolving granulated sugar in 200 g of water. Next, the green tea extract and syrup that were obtained in Example 5 were added to 100 g of water, heated to 145 ° C. with stirring, then turned off, 50 wt% tartaric acid was added, and the mixture was further mixed with granulated sugar aqueous solution. It cooled to 75-80 degreeC, and shape | molded with the forming roller, and the candy of the following composition was prepared.
520g of granulated sugar
Minamata 230g
25 g of green tea extract of Example 5
Orange fragrance 1g
50g% tartaric acid 10g
300 g of water

Example 8 Preparation of Tea Polyphenols-Containing Beverage In accordance with a conventional method, the following raw materials other than 50 wt% citric acid solution and orange flavor were dissolved in water while stirring. Subsequently, the pH of the solution was adjusted to pH 3.1 using a 50 wt% citric acid solution, the fragrance was added after the temperature was raised to 95 ° C., and the total amount was adjusted to 1 liter with water. The obtained solution was filled into 100 ml bottles and cooled to prepare a beverage having the following formulation.
Fructose glucose liquid sugar 120g
1/5 concentrated orange juice 10g
1/5 clear lemon juice 4g
0.5 g of sodium citrate
Green tea extract of Example 5 0.5g
50% citric acid solution pH adjustment orange fragrance 0.1g
Appropriate amount of water 1 liter

Test Example 1: Anti-stress action test As an anti-stress action test, 36 accounting clerks using a computer for 6 hours or more per day were allowed to ingest 6 tablets per day for 2 weeks. After that, we conducted a questionnaire survey on various symptoms. The results of evaluating the anti-stress effect of the product of the present invention are shown in Table 1. In Table 1, for the symptoms before and after the test, the number of persons who felt the corresponding symptoms is shown.

  From the above results, 60% or more of all items felt improvement in the symptoms complained before taking the product of the present invention, and the anti-stress action of the product of the present invention became clear.

Test Example 2: Test in Renal Disease Patient 51 dialysis patients were allowed to take 6 tablets of Example 6 per day for 4 months. Hematological properties before and after the test were confirmed (see Table 2), and a questionnaire survey was conducted for various symptoms (see Table 3) to evaluate the antistress action of the present invention. As hematological properties, creatinine concentration, β-microglobulin concentration and ratio of creatinine to methylguanidine (MG / Cr ratio) were confirmed. In Table 3, the symptom before and after the test indicates the number of people who felt the symptom. For reference, the normal value of each evaluation item is creatine: male 0.7 to 1.2 mg / dl, female 0.5 to 0.9 mg / dl, and β-microglobulin: 0.5 to 1.5 mg / dl. dl.

  From the above results, improvement of psychosomatic symptoms, creatinine concentration and hepatic evaluation of dialysis patients, ratio of creatinine and methylguanidine, β-microglobulin concentration that is an indicator of pain, etc. Thus, the anti-stress action of the present invention was clarified.

Test example 3 Test in smoking rats In order to fix the rat on its back and introduce cigarette smoke into the lungs through the airway, an introduction tube was inserted into the airway of the rat, and a blood collection catheter was placed in the vein so that blood could be collected from the vein. Inserted into.

  A cigarette containing 1.2 mg of nicotine per fire is lit, smoke is introduced into the lungs with a pump, and the active oxygen species in the blood 3 hours after introduction is analyzed by chemiluminescence analyzer (CLA-SP2, Tohoku Electronics Industry Co., Ltd.) ) And counting chemiluminescence per 10 seconds. The results are shown in Table 4.

  In the test group, the non-administration group (control) of the product of the present invention and the product D of the present invention obtained in Example 4 were orally administered at 10 mg / kg body weight one hour before the introduction of tobacco smoke. The test group was divided into three test groups, and the group administered the same amount once a day for one week.

  From the above results, the reactive oxygen species serving as an index of the stress state caused by smoking decreased, and the anti-stress action of the present invention became clear.

  In order to elucidate this mechanism, the activity of active oxygen scavenging enzymes (CuZnSOD, MnSOD and catalase) in the rat lung was measured using SDS-PAGE after the test, and no tobacco smoke was introduced. It was found that the activity of the reactive oxygen scavenging enzyme was remarkably reduced in the rats in which tobacco was forcibly introduced and the composition of the present invention was not administered as compared with the rats. However, the activity in the single administration group was slightly decreased, and the activity in the continuous administration group for one week was almost the same as that of the rat not introduced with tobacco smoke. From this, it was speculated that the reduction of smoking stress according to the present invention was induced by suppressing the decrease in the activity of the active oxygen removing enzyme in the lung.

  According to the present invention, an inexpensive anti-stress composition that is effective in alleviating mental and physical symptoms when in a stress state, taking into account the increase in stress in modern society and its impact on living bodies, and being safe and readily available Can be provided.

Claims (4)

  An anti-stress composition comprising a material containing tea polyphenols.   The anti-stress composition of Claim 1 for relieving the psychosomatic symptom in the treatment of a renal disease.   The anti-stress composition according to claim 1 or 2, which is a food or a medicine.   The antistress composition according to claim 3, which is a tablet or a capsule.