JP2005281174A - Active oxygen eliminating agent - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an active oxygen eliminating agent which is highly safe and excellent in action of eliminating active oxygen. <P>SOLUTION: The active oxygen eliminating agent contains, as the effective component, a water extract and/or an organic solvent extract extracted from Carpinus laxiflora, i.e., a leaf fall tree of the genus Carpinus japonica of Betulaceae. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to an active oxygen scavenger containing a plant extract as an active ingredient, and more particularly to an active oxygen scavenger having an excellent scavenging action against hydrogen peroxide which is deeply involved in skin aging and the like.

In recent years, various effects of active oxygen on a living body have been attracting attention against the background of increasing health orientation. Active oxygen is an oxygen that is significantly more susceptible to chemical reaction than oxygen in the normal ground state ( ³ O ₂ , triplet). Specifically, superoxide anion radical (· O ₂ ⁻ ), excess oxygen Reactive oxygen such as hydrogen oxide (H ₂ O ₂ ), hydroxyl radical (.OH), hydroperoxyl radical (.OOH), singlet oxygen ( ¹ O ₂ ), etc. are collectively called active oxygen. . This active oxygen is known to be generated through exposure to ultraviolet rays, leukocyte sterilization mechanism, energy metabolism process in living cells, stress and the like.

  Active oxygen is involved in the bactericidal action in the living body and plays an important role in defense of the living body, but also has an undesirable effect on the living body. For example, it is well known that skin aging such as skin wrinkles, dullness, loss of texture, loss of elasticity, pigmentation (stains, freckles, etc.) is caused by ultraviolet rays. It is said that oxygen is involved. Specifically, when a living body is exposed to ultraviolet rays, catalase in the skin, an enzyme that decomposes hydrogen peroxide, is easily deactivated, and a large amount of excess from leukocytes that infiltrate the inflamed tissues due to ultraviolet rays. It is presumed that hydrogen peroxide is released and hydrogen peroxide accumulates in the skin exposed to ultraviolet rays, which accelerates skin aging.

Hydrogen peroxide is generated not only for the above-mentioned causes but also as a result of SOD (superoxide dismutase) erasing the superoxide anion radical (.O ₂ ⁻ ) in the living body. This hydrogen peroxide is a long-lived active oxygen species, and is known to be converted into the most harmful hydroxyl radical among active oxygen by the catalytic action of monovalent copper ions and divalent iron ions. ing. It has been clarified that hydroxyl radicals cause denaturation, decomposition and polymerization of skin extracellular matrix components such as collagen, elastin and hyaluronic acid. Therefore, if hydrogen peroxide generated in the skin is left unattended, hydroxyl radicals are generated, and as a result, skin aging is promoted. In addition, hydrogen peroxide has been confirmed to be a major obstacle to dermal fibroblasts that produce skin constituents and has a strong DNA damaging effect.

In addition to promoting skin aging as described above, active oxygen induces cancer by damaging nucleic acids, proteins, lipids, etc., and various diseases such as cataract, arteriosclerosis, Alzheimer's disease and asthma It has become clear that it is involved in the onset or worsening.
As described above, the active oxygen generated more than necessary in the living body causes various harmful effects on the living body. Therefore, erasing excess active oxygen in the living body prevents or improves the adverse effects related to the active oxygen. Is very important above.
Furthermore, active oxygen has an undesirable effect not only on living organisms but also on various products. For example, various components contained in foods and drinks, cosmetics, pharmaceuticals and the like are oxidized to promote the deterioration of their quality, the generation of off-flavors, the occurrence of discoloration and fading.
Therefore, many active oxygen scavengers have been reported in the past in order to eliminate such various problems caused by active oxygen. In recent years, from the viewpoint of safety, studies have been actively conducted to obtain a substance having an active oxygen scavenging action from a natural product (for example, Patent Documents 1 to 3).

JP-A-2-264727 Japanese Patent Laid-Open No. 3-221588 JP-A-4-202138

However, conventional active oxygen scavengers obtained from natural products have no problem in terms of safety, but are not always satisfactory in terms of active oxygen scavenging action.
Therefore, an object of the present invention is to provide an active oxygen scavenger that has an excellent scavenging effect on active oxygen and has high safety.

In order to solve the above-mentioned problems, the present inventor has conducted extensive studies on extracts of many natural products. It has been found that there is an oxygen scavenging action, particularly an excellent scavenging action on hydrogen peroxide, and based on this finding, the present invention has been completed.
That is, the present invention is an active oxygen scavenger characterized by containing, as an active ingredient, water and / or an organic solvent extract from red pine (Carpinus laxiflora) which is a deciduous tree of the genus Kumashide.

The active oxygen scavenger of the present invention is different from many conventionally known active oxygen scavengers which mainly have a function of scavenging superoxide anions and / or hydroxyl radicals among active oxygens. It has the feature that the erasing action is very strong. Therefore, the active oxygen scavenger of the present invention can effectively prevent and improve aging of skin (including hair) in which hydrogen peroxide is particularly involved.
In addition, some of the conventional active oxygen scavengers are oxidized by reacting with active oxygen, and as a result, themselves are converted into causative substances that cause damage to the living body. The active oxygen scavenger of the present invention erases the energy of active oxygen in the form of light emission, so this risk is low, and it is different from conventional similar products in terms of safety to the living body. It is a highly useful active oxygen scavenger.
Thus, the active oxygen scavenger of the present invention not only has a high scavenging ability against active oxygen, particularly hydrogen peroxide, but also has excellent safety, so that it is intended for humans and non-human animals. It is suitable for prevention and treatment of various diseases or prevention of deterioration of components contained therein as an antioxidant, for example, by blending in cosmetics, foods and drinks, and pharmaceuticals.

As described above, the present invention is an active oxygen scavenger characterized by containing, as an active ingredient, water and / or an organic solvent extract from red pine (Carpinus laxiflora), which is a deciduous tree of the genus Anemoneaceae. .
The extract from red pepper which is an active ingredient of the present invention means an extract obtained using red pepper as an extraction raw material, and is obtained by drying the extract, a diluted or concentrated liquid of the extract, and the extract. The dried product or any of these crudely purified products or purified products is included in the extract from red peach.

Akashide used as an extraction raw material is a deciduous Takagi belonging to the genus Anemoneaceae, which is distributed in the mountains of Hokkaido (south), Honshu, Shikoku, and Kyushu (Kirishimayama) and is easily available from these areas. is there.
The part of red peach used as an extraction raw material is not particularly limited, and examples thereof include bark, root bark, leaves, fruit spikes, and branches. Use of bark, root bark, and leaves is preferable because the active oxygen scavenging activity of the extract is high.

An extract of red pepper having active oxygen scavenging action can be easily obtained by a method generally used for plant extraction. That is, an extract of red pepper can be obtained by drying red pepper, which is the raw material for extraction, as it is or after pulverization and subjecting it to extraction with an extraction solvent. Akaside may be dried in the sun or using a commonly used dryer.
In addition, the details of the substance (group) having an active oxygen scavenging action contained in the extract of red pepper are unknown. From the results of Examples described later, it is presumed that a component specific to the extract of Akaside that is not included in birch trees other than Akashide is involved in the active oxygen scavenging action of the present invention.

  As the extraction solvent, water (including hot water) or an organic solvent is used. Examples of the organic solvent include lower aliphatic alcohols such as methanol and ethanol, halogenated hydrocarbons such as methylene chloride and chloroform, ethers such as dimethyl ether, diethyl ether and methyl ethyl ether, ethyl acetate, propyl acetate and butyl acetate. Esters, lower aliphatic ketones such as acetone, and hydrocarbons such as hexane, heptane and benzene. As the extraction solvent, water (including hot water), a hydrophilic organic solvent, or a mixture thereof is preferably used at room temperature or below the boiling point of the solvent. Specific examples thereof include water, lower aliphatic alcohol, And lower aliphatic alcohols. These can be used alone or as a mixture of two or more thereof. Specific examples of suitable lower aliphatic alcohols include methanol, ethanol, propanol, 1,3-butylene glycol, glycerin, propylene glycol and the like.

  Water that can be used as the extraction solvent includes pure water, tap water, well water, mineral spring water, mineral water, hot spring water, spring water, fresh water, and the like, as well as those obtained by subjecting these waters to various treatments. Examples of the treatment include purification, heating, sterilization, sterilization, filtration, ion exchange, adjustment of osmotic pressure, buffering, and the like. Therefore, the water that can be used as the extraction solvent in the present invention includes purified water, hot water, ion-exchanged water, physiological saline, phosphate buffer, phosphate buffered saline, and the like.

  When using the liquid mixture of 2 or more types of polar solvents as an extraction solvent, the mixing ratio can be adjusted suitably. For example, when a mixed liquid of water and a lower aliphatic alcohol is used, the mixing ratio of water and the lower aliphatic alcohol can be freely changed, but in particular 7: 3 to 2: 8 (mass ratio). ) Is preferred.

The extraction process of red pepper is not particularly limited and can be performed according to a conventional method, and any apparatus can be used at room temperature or under reflux heating.
For example, red pepper as an extraction raw material is put into a container filled with the extraction solvent, and the soluble components are eluted with occasional stirring. At this time, the extraction conditions can be appropriately adjusted according to the part of the extraction raw material and the like, but the amount of the extraction solvent is usually 20 to 50 times (mass ratio) of the extraction raw material, and the extraction time is usually heat. In the case of water, it takes about 1 hour at 85 to 90 ° C., in the case of room temperature water, it takes about 24 hours, and in the case of organic solvents such as lower aliphatic alcohols and hydrous lower aliphatic alcohols, it takes 2 to 4 days at room temperature.

After elution of soluble components by the extraction process, the processed liquid is filtered to remove the extraction residue, thereby obtaining an Akaside extract. The obtained extract can be a diluted or concentrated solution of the extract, or a dried product of the extract. Furthermore, in order to obtain these crudely purified products or purified products, Treatments such as dilution, concentration, drying, and purification may be performed according to conventional methods.
The obtained extract can be used as it is as the active oxygen scavenger of the present invention, but its concentrated solution or dried product is preferable in terms of handling. In obtaining the dried extract, a carrier such as dextrin or cyclodextrin may be added to improve the hygroscopicity.

  If necessary, the obtained extract may be purified for the purpose of improving the active oxygen scavenging activity, decoloring, deodorizing, etc., as long as the active oxygen scavenging activity is not lowered. Specific examples of the purification method include activated carbon treatment, adsorption resin treatment, ion exchange resin treatment, and the like.

  The extract from red peach can be formulated and used according to a conventional method. In the case of formulating, a pharmaceutically acceptable carrier such as dextrin and cyclodextrin, and other optional auxiliaries can be added to facilitate storage and handling. The extract from red pepper can be made into any dosage form such as powder, granule, tablet, etc. by formulation.

  The active oxygen scavenger of this invention can be mix | blended with arbitrary cosmetics. The types of the cosmetics are not particularly limited, and specific examples thereof include ointments, creams, emulsions, lotions, packs, foundations, eye shadows, mascaras, nail polish, lipsticks, bath salts, hair arts, hair liquids, hair lotions. , Hair spray, hair conditioner, hair treatment, soap, body shampoo, shampoo, rinse, perfume and the like. As long as the active oxygen scavenging action is not hindered, various main ingredients and auxiliaries usually used in the production of cosmetics and other optional components can be blended in the cosmetic.

  The amount of the active oxygen scavenger of the present invention in the cosmetic can be appropriately adjusted depending on the type of cosmetic and the activity of the active oxygen scavenging action of the red pepper extract. It is about 0.0001 to 10% by mass in terms of the red pepper extract relative to the entire cosmetic.

  In the above cosmetics, together with the active oxygen scavenger of the present invention, those usable as components include various astringents, bactericides / antibacterial agents, ultraviolet absorbers, whitening agents, moisturizers, cell activators, anti-inflammatory / Examples include antiallergic agents, antioxidant / active oxygen scavengers, fats and oils, waxes, hydrocarbons, fatty acids, alcohols, esters, surfactants, fragrances and the like. In addition, when these structural components are used in combination with the red pepper extract, a synergistic action between the red pepper extract and the combined constituents may cause an excellent use effect that is more than normally expected.

  Examples of the astringent include citric acid or a salt thereof, tartaric acid or a salt thereof, lactic acid or a salt thereof, aluminum chloride, potassium aluminum sulfate, allantoinchlorohydroxyaluminum, allantoindihydroxyaluminum, zinc paraphenolsulfonate, zinc sulfate, diu extract, Agetsu Extract, Hamamelis extract, Gennoshoco extract, Tea catechins, Proanthocyanidins, Guillain extract, Odrianthus extract, Hypericum extract, Dianthus extract, Cornflower extract, Horsetail extract, Kizuta extract, Cucumber extract, Marronie extract, Salvia extract, Melissa extract, Tamarind Husk extract Etc.

  Examples of the bactericidal and antibacterial agents include benzoic acid, sodium benzoate, paraoxybenzoic acid ester, distearylmethylammonium chloride, benzethonium chloride, alkyldiaminoethylglycine chloride solution, chlorhexidine hydrochloride, orthophenylphenol, photosensitizer 101, photosensitizer No. 201, salicylic acid, sodium salicylate, sorbic acid, halocarban, resorcin, parachlorophenol, phenoxyethanol, bisabolol, hinokitiol, menthol, chitosan, chitosan degradation product, scallop extract, cucumber extract, enmiso extract, loquat extract, walrus extract, hop extract, Yucca extract, aloe extract, cinnamon bark extract, gadget extract, oil-soluble licorice extract (licorice hydrophobic flavonoids, grabrizine, glabrene, lycocar Emissions A), and the like.

  Examples of the ultraviolet absorber include β-isopropylfuranone derivatives, urocanic acid, ethyl urocanate, oxybenzone, oxybenzenesulfonic acid, tetrahydroxybenzophenone, dihydroxydimethoxybenzophenone, dihydroxybenzophenone, cinoxalate, methyl diisopropylcinnamate, methoxycinnamic acid Octyl, glyceryl paraaminobenzoate, amyl paradimethylaminobenzoate, octyl paradimethylbenzoate, octyl paraaminobenzoate, paraaminobenzoic acid, ethyl paraaminobenzoate, butylmethoxydibenzoylmethane, titanium oxide, β-carotene, γ-oryzanol , Rice bran extract, aloe extract, chamomile extract, kogomegusa extract, hawthorn extract, henna extract, teuchigurumi extract Horse chestnut extract, ginkgo leaf extract, oil-soluble licorice extract and the like.

  Examples of the whitening agent include ascorbic acid and derivatives thereof, sulfur, placental hydrolyzate, ellagic acid and derivatives thereof, kojic acid and derivatives thereof, glucosamine and derivatives thereof, azelaic acid and derivatives thereof, arbutin and derivatives thereof, hydroxycinnamic Acids and derivatives thereof, glutathione, arnica extract, urgonum extract, senkyu extract, scorpion extract, psycho extract, boufu extract, hamboufu extract, garlic mycelium culture and extract thereof, gymnema extract, linden extract, peach leaf extract, age extract , Kujin extract, scallop extract, toki extract, yakuinin extract, oyster leaf extract, daiou extract, button pi extract, hamamelis extract, marronnier extract, hypericum extract, licorice extract, oil-soluble licorice extract Licorice hydrophobic flavones, glabridin, glabrene include licochalcone A) or the like.

  Examples of the moisturizer include serine, glycine, threonine, alanine, collagen, hydrolyzed collagen, vitronectin, fibronectin, keratin, elastin, royal jelly, chondroitin sulfate heparin, glycerophosphate lipid, sphingophospholipid, sphingoglycolipid, linoleic acid or its Esters, eicosapentaenoic acid or esters thereof, pectin, algae colloid, bifidobacteria fermentation product, lactic acid fermentation product, yeast extract, litchi mycelium culture or extract thereof, wheat germ oil, avocado oil, rice germ oil, Jojoba oil, soybean phospholipid, γ-oryzanol, bellows oyster extract, Yokuinin extract, sage extract, taisou extract, diatom extract, burdock extract, maroonie extract, mannen wax extract, arnica Kiss, wheat bran, rice bran, and the like.

  Examples of the cell activator include placental extract, riboflavin or derivative thereof, pyridoxine or derivative thereof, nicotinic acid or derivative thereof, pantothenic acid or derivative thereof, α-tocopherol or derivative thereof, arnica extract, carrot extract, ginseng extract, sorghum Extract, loofah extract (saponin), sicon extract, white birch extract, agate extract, button pea extract, peonies extract, mugwort extract, safflower extract, ashitaba extract, loquat leaf extract, hyacinth extract, saxifrage extract, japonica extract, salvia extract, garlic extract And mannen wax extract.

  Examples of the anti-inflammatory and antiallergic agents include azulene, allantoin, aminocaproic acid, indomethacin, lysozyme chloride, epsilon aminocaproic acid, oxybenzone, glycyrrhizic acid or a derivative thereof, glycyrrhetinic acid or a derivative thereof, photosensitive element 301, photosensitive element 401, hydrochloric acid Diphenhydramine, tranexamic acid or derivatives thereof, adenosine phosphate, estradiol, estrone, ethinyl estradiol, cortisone, hydrocortisone, prednisolone, progesterone, corticosterone, arnica extract, inchinko extract, sanshishi extract, deer extract, licorice extract, toki extract, Artemisia extract, Walnut extract, Gentian extract, Psycho extract, Senkyu extract, Bow-fu extract, Achillea millefolium Extract, Coptis extract, mint extract, horse chestnut extract, scutellaria root extract, and the like.

  Examples of the antioxidant / active oxygen scavenger include dibutylhydroxytoluene, butylhydroxyanisole, propyl gallate, baicalin, baicalein, superoxide dismutase, catalase, melissa extract, age extract, loquat leaf extract, hop extract, peony extract. , Sage extract, quina extract, chamomile extract, eucalyptus extract, perilla extract, ginkgo biloba extract, thyme extract, cardamom extract, caraway extract, nutmeg extract, mace extract, laurel extract, clove extract, turmeric extract, willow tree extract and the like.

  The above oils include soybean oil, linseed oil, tung oil, sesame oil, nutka oil, cottonseed oil, rapeseed oil, safflower oil, corn oil, olive oil, camellia oil, almond oil, castor oil, peanut oil, cacao oil, molasses Coconut oil, palm kernel oil, beef tallow, mink oil, egg yolk oil, jojoba oil, evening primrose oil, horse oil and the like.

  Examples of the waxes include carnauba wax, candelilla wax, beeswax, white beeswax, whale wax, serax, lanolins and the like.

  Examples of the hydrocarbons include liquid paraffin, petrolatum, microlistin wax, ceresin, squalane, and polyethylene powder.

  Examples of the fatty acids include stearic acid, linoleic acid, lauric acid, myristic acid, palmitic acid, hebenic acid, lanolinic acid, oleic acid, undecylenic acid, and isostearic acid.

  Examples of the alcohols include lauryl alcohol, cetyl alcohol, stearyl alcohol, lanolin alcohol, hydrogenated lanolin alcohol, oleyl alcohol, hexadecyl alcohol, 2-octyldodecanol, glycerin, sorbitol, propylene glycol, 1,3-butylene glycol, Examples include ethylene glycol or a polymer thereof, glucose, sucrose, cholesterol, phytosterol, and cetostearyl alcohol.

  Examples of the esters include decyl oleate, butyl stearate, myristyl myristate, hexyl laurate, isopropyl palmitate, isopropyl myristate, octyldodecyl myristate, hexyl decyl dimethyloctanoate, propylene glycol dioleate, diethyl phthalate, Examples include propylene glycol monostearate, ethylene glycol monostearate, glyceryl monostearate, glyceryl trimyristate, lanolin acetate, cetyl lactate, and the like.

  Examples of the surfactant include an anionic surfactant, a cationic surfactant, an amphoteric surfactant, and a nonionic surfactant.

  Examples of the fragrance include menthol, carvone, eugenol, anethole, peppermint oil, spearmint oil, peppermint oil, eucalyptus oil, anise oil, and other oily fragrances from various animals and plants.

  The active oxygen scavenger of this invention can also be mix | blended with arbitrary food-drinks (a nutritional supplement is included). In this case, it is appropriate to adjust the blending amount so that the daily intake of the red pepper extract is about 1-1000 mg per day in consideration of the general intake amount of the food and drink to be added.

  Although the food and drink which can mix | blend the active oxygen scavenger of this invention is not specifically limited, As the specific example, drinks, such as a soft drink, a carbonated drink, a nutritive drink, a fruit drink, and a lactic acid drink (Concentrated undiluted solution of these drinks and Including ice cream, ice sherbet, shaved ice, etc .; noodles such as buckwheat, udon, harusame, gyoza skin, cucumber skin, Chinese noodles, instant noodles; rice cake, chewing gum, candy, gum, chocolate , Biscuits such as tablet confectionery, snack confectionery, confectionery such as jelly, jam, cream, baked confectionery, fishery products such as kamaboko, ham, sausage, processed livestock products; dairy products such as processed milk, fermented milk; salad oil, tempura oil, Margarine, mayonnaise, shortening, whipped cream, dressing and other fats and oils and processed foods; sauces, sauces and other seasonings Soup, stew, salad, prepared foods, pickles, and the like.

  Furthermore, the active oxygen scavenger of the present invention can be blended in any pharmaceutical (including various supplements, health foods, and quasi drugs). The compounding amount in that case is not particularly limited, and may be appropriately determined in consideration of the dosage form, patient age, body weight, administration route, and the like. The kind of said pharmaceutical is not limited, A tablet, a capsule, a granule, an injection, an ointment, a liquid agent, a dentifrice, a bad breath prevention agent, a hair restorer, a hair dye, etc. can be selected widely. The active oxygen scavenger of the present invention can be applied not only to humans but also to animals other than humans.

  Hereinafter, the present invention will be described in detail with reference to production examples and test examples, but the present invention is not limited thereto.

(Production Example 1)
2 g each of bark, root bark, and leaf crude product of red pine (Carpinus laxiflora) was put into 100 ml of extraction solvent (hot water at 85 to 90 ° C.), and extraction was performed for 1 hour with occasional stirring. Thereafter, each extraction-treated product was filtered, and each filtrate was freeze-dried to obtain an Akaside extract.
The yield of each extract ((freeze-dried material amount / crushed material amount) × 100, the same as in Production Example 2) was 11.1% for bark, 15.5% for root bark, and 26.1% for leaf. there were. For each test described below, a solution prepared by dissolving the obtained red pepper extract in purified water to a final concentration of 0.1% (w / w) was used.

(Production Example 2)
2 g each of bark, root bark, and leaf crude of red pine (Carpinus laxiflora) were put into 80 ml of extraction solvent (room temperature water), and extracted for 24 hours with occasional stirring. Thereafter, each extraction-treated product was filtered, and each filtrate was freeze-dried to obtain an Akaside extract.
The yield of each extract was 9.6% for bark, 12.3% for root bark, and 15.0% for leaf. In the test described below, a solution prepared by dissolving the obtained red pepper extract in purified water to a final concentration of 0.1% (w / w) was used.

(Production Example 3)
2 g each of bark, root bark, and leaf crushed material of red pine (Carpinus laxiflora) were put into 60 ml of an extraction solvent (50% (w / w) ethanol) and extracted at room temperature for 3 days with occasional stirring. Thereafter, each extraction-treated product was filtered, and each filtrate was dried under reduced pressure at 40 ° C. to obtain an Akaside extract.
The yield of each extract ((the amount of dried solid substance / crushed substance) × 100) was 12.5% for bark, 16.0% for root bark, and 33.3% for leaves. In the test described below, a solution obtained by dissolving the obtained red pepper extract in 50% (w / w) ethanol to a final concentration of 0.1% (w / w) was used.

(Test Example 1)
Evaluation of the hydrogen peroxide scavenging action of each red pepper extract obtained in Production Examples 1 to 3 is performed when active oxygen scavenging substance, receptor substance (also called mediator) and active oxygen are reacted. This was carried out by applying the phenomenon that active oxygen energy is converted and released into visible light energy according to the action intensity of the erasing substance, and the active oxygen is eliminated (Phytochemistry, Vol.39, No.1, 225-229, 1995, J Biolumin. And Chemilumin., Vol. 11, No. 3, 131-136, 1996, Japan Food Science, Vol. 38, No. 8, 18-21, 1999).
As receptor substances, it is already known that carbonates, in particular potassium hydrogen carbonate, are excellent (see Japan Food Science, Vol 38, No. 12, 13-20, 1999). Are widely distributed molecules. In addition, it has been clarified that each organ of a living body including a skin itself has a function as a receptor substance (Japan Food Science, Vol 39, No. 3, 17-24, No. 4). , 17-24, 2000). Therefore, it is considered that a substance having a strong active oxygen scavenging action can effectively erase active oxygen species generated in the body in cooperation with these receptor substances when applied to a living body. Yes.
Specifically, the hydrogen peroxide scavenging action was evaluated by extracting 0.2 ml of a 3% (w / w) aqueous hydrogen peroxide solution into a test tube and 0.1% (w / w) red pine bark obtained in Production Example 1. 0.5 ml of the product solution and 0.3 ml of 2 mol potassium hydrogen carbonate aqueous solution were sequentially dispensed, and immediately, using a luminescence sensor PSN AB2200 (trade name, manufactured by Ato Inc.), the amount of light emitted for 10 minutes in 10 second increments. The total number of photons was measured over time. The higher this value, the stronger the action of converting the energy of hydrogen peroxide into light energy, that is, the hydrogen peroxide erasing action.
Furthermore, in order to compare the intensity of the hydrogen peroxide scavenging action by tree species, five types of representative trees belonging to the same birch family as Akashide, namely, Sowashiba (Kanobiaceae) and Kumashide (Kanshide) Genus), Yashabushi (birchaceae alder genus), Yamanoki (birchaceae alder genus), and birch (birchaceae birch genus) are prepared in the same manner as in Production Example 1, and the luminescence amount of each extract solution. Was measured as described above. Each extract solution was measured three times, and the measured values were summarized as a total light emission amount for 10 minutes, and the results were expressed as average values ± standard deviation and relative values. The measurement results are shown in Table 1.

(Test Example 2)
In Test Example 1, instead of the 0.1% (w / w) Akashide bark extract solution obtained in Production Example 1, the 0.1% (w / w) Akashide bark extract solution obtained in Production Example 2 was used. In the same manner as in Test Example 1, except that the bark extract solution of the control tree prepared in the same manner as in Production Example 2 was used instead of the bark extract solution in the same manner as in Production Example 1. Then, the amount of luminescence of each extract solution was measured. The measurement results are shown in Table 2.

(Test Example 3)
In Test Example 1, instead of the 0.1% (w / w) red pepper bark extract solution obtained in Production Example 1, the 0.1% (w / w) red pepper root bark extract solution obtained in Production Example 2 And in place of the bark extract solution of the control tree prepared in the same manner as in Production Example 1, the same method as in Test Example 1 was used except that the bark extract solution of the control tree prepared in the same manner as in Production Example 2 was used. Thus, the amount of luminescence of each extract solution was measured. The measurement results are shown in Table 3.

(Test Example 4)
In Test Example 1, instead of the 0.1% (w / w) Akashide bark extract solution obtained in Production Example 1, the 0.1% (w / w) Akashide leaf extract solution obtained in Production Example 3 was used. And in the same manner as in Test Example 1, except that the control tree leaf extract solution prepared in the same manner as in Production Example 3 was used instead of the bark extract solution in the same manner as in Production Example 1. The amount of luminescence of each extract solution was measured. The measurement results are shown in Table 4.

(Evaluation)
As is clear from the results of Test Examples 1 to 4 above, the red pepper extract exhibits a very high value for the total amount of luminescence, and this birch species has a strong hydrogen peroxide scavenging action in the birch tree. It was confirmed that From this, it is presumed that a component specific to the extract of Akaside that is not contained in birch trees other than Akaside is involved in the hydrogen peroxide scavenging action.

(Test Example 5)
Further, for comparison purposes, after adjusting the concentration of a commercially available plant extract, which is supposed to have a hydrogen peroxide scavenging action, to 0.1% (w / w), The amount of luminescence of the extract solution was measured. The commercially available plant extracts used for the measurement are Hamelis extract (Hamamelis extract BG-J, manufactured by Maruzen Pharmaceutical Co., Ltd., trade name), Green tea extract A (Green tea extract, manufactured by Maruzen Pharmaceutical Co., Ltd., trade name), Buttonpi extract ( Buttonpi extract, Maruzen Pharmaceutical Co., Ltd., trade name), Rosemary extract (Rosemary Extract BG, Maruzen Pharmaceutical Co., trade name), Ogon extract (Ogon Extract BG, Maruzen Pharmaceutical Co., trade name), green tea Extract B (green tea liquid, manufactured by Ichimaru Falcos, trade name), olive fruit extract (manoliva, manufactured by Sepic (French), trade name). Each extract solution was measured three times, and the measurement result was expressed as an average value ± standard deviation. The measurement results are shown in Table 5.

(Evaluation)
As is clear from the results of Test Example 5 above, the Hamelis extract shows the highest value for the total amount of luminescence, and among the commercially available plant extracts, it has a very strong hydrogen peroxide scavenging action. It was revealed.

(Test Example 6)
Aqueside bark hot water extract solution obtained in Production Example 1, Akaside bark water extract solution obtained in Production Example 2, Akaside root bark water extract solution, and 50% of Akashide leaves obtained in Production Example 3 The measured value of the total amount of luminescence of the (w / w) ethanol extract solution was compared with the measured value of the Hamelis extract solution that showed the strongest hydrogen peroxide scavenging action in Test Example 5. The results are shown in Table 6.

(Evaluation)
As is clear from the results of Test Example 6 above, the four kinds of red peanut extracts all showed clearly higher values for the total amount of luminescence than the commercially available Hamelis extract. From this result, it was confirmed that the red pepper extract had a much superior hydrogen peroxide scavenging action than the existing plant extract.

Claims (5)

  An active oxygen scavenger characterized by containing water and / or an organic solvent extract from a deciduous tree, Carpinus laxiflora, as an active ingredient.   2. The active oxygen scavenger according to claim 1, wherein the active oxygen is hydrogen peroxide.   A cosmetic comprising the active oxygen scavenger according to claim 1 or 2.   A food or drink comprising the active oxygen scavenger according to claim 1 or 2. A pharmaceutical product comprising the active oxygen scavenger according to claim 1 or 2.