JP2005029556A - Skin aging-preventing agent - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To obtain a skin aging-preventing agent capable of inhibiting the activities of elastase and hyaluronidase, recovering/maintaining the tension and elasticity of skin, and further having an anti-allergy effect, a safe and highly effective hyaluronidase and elastase inhibitor, and further a cosmetic composition and a food composition blended with the same. <P>SOLUTION: The hyaluronidase and elastase inhibitor contains pipsissewa (Chimaphila umbellata, L), and the cosmetic composition and food composition are obtained by blending the same. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

The present invention is a composition comprising a food composition, a cosmetic composition, a pharmaceutical composition, and the like containing, as an active ingredient, Chimophila umbellata (L.) WPCBarton, which is a plant belonging to the genus Chimaphila Pursh. And relates to a skin anti-aging agent.
The present invention also relates to an elastase inhibitor and a hyaluronidase inhibitor containing, as an active ingredient, chimaphila umbellata (L.) WPCBarton, which is a plant belonging to the genus Chimaphila Pursh. Further, it is a composition comprising a food composition, a cosmetic composition, a pharmaceutical composition and the like containing the inhibitor, and relates to a skin aging inhibitor, and has an inhibitory effect on giant pea (Chimaphila umbellata (L.) WPCBarton). The present invention relates to a composition used and a method of using the composition in its application field.
Furthermore, the present invention relates to a method for producing an elastase inhibitor and a hyaluronidase inhibitor, which contains chimaphila umbellata (L.) WPCBarton as an active ingredient.

  Typical changes in human skin aging are wrinkles and tarmi, which is one of the major skin problems for older people and women. However, at present, there are few effective treatments for skin wrinkles and tarmi, and various studies on aging have been conducted in the aging society. The formation of wrinkles and tarmi on human skin is thought to be caused by a decrease in the elasticity of the skin, and factors such as aging, drying, oxidation, and the effects of sunlight (ultraviolet rays) are related. A typical histological phenomenon is caused by changes in extracellular matrix components such as elastin, collagen, and glycosaminoglycan in the dermis, particularly changes in elastin. In other words, the decrease in skin elasticity that occurs during the formation of wrinkles and tarmi is considered to be due to the elastase produced and secreted from dermal fibroblasts being involved in the modification of the three-dimensional structure caused by elastin injury. However, preventing the degeneration of elastin that maintains the elasticity and firmness of the skin prevents the formation of wrinkles and tarmi, that is, prevents the aging of the skin.

  On the other hand, dry skin, rough skin, firmness, decreased elasticity, and increased spots and wrinkles are closely related to the degradation of hyaluronic acid. Hyaluronic acid is a type of mucopolysaccharide that is abundant in tissues such as skin, ligaments, joint fluid, and vitreous body of the eye. For example, in the skin and oral cavity, it protects cells, transports nutrients, retains tissue moisture, and softens. It plays an important role in maintaining sexuality, as well as maintaining tissue structure and function as a joint fluid and maintaining lubricity. Since the amount of living hyaluronic acid in the skin, oral cavity, and joints decreases due to aging or pathological conditions, inhibiting hyaluronidase, a hyaluronic acid hydrolase, suppresses the degradation of hyaluronic acid and maintains the amount of living hyaluronic acid Contribute to.

Furthermore, hyaluronidase inhibitors can be used as anti-inflammatory and anti-allergic agents caused by hyaluronidase. This is because hyaluronidase is activated during inflammation, destroys the matrix of connective tissue, infiltrates inflammatory tissues, enhances vascular permeability, and mediates the process of histamine release from mast cells in type I allergy. It is highly likely that
Regarding elastase activity inhibition, skin aging can be prevented if excessive action of elastase can be suppressed. However, conventionally reported elastase inhibitors are not always satisfactory in terms of stability, effect, and feeling of use. As for the inhibition of hyaluronidase activity, various searches have been made for raw materials having anti-inflammatory or anti-allergic activity, but safe and promising materials have not yet been obtained. It is a natural product from the aspect of safety to human skin, a substance that has been eaten by humans for many years and has been guaranteed in terms of safety, has a strong hyaluronidase inhibitory action, and also has other effects on the skin. It is desired.

  The present invention provides an anti-aging agent for skin that inhibits the activity of elastase and hyaluronidase, prevents skin aging by restoring and maintaining the elasticity and elasticity of the skin, and further has anti-inflammatory and anti-allergic effects. The purpose is to do.

  As a result of intensive studies to solve the above problems, the present inventors can provide an extremely effective and effective method for preventing skin aging, and a plant belonging to the genus Chimaphila Pursh. Thus, a method for preventing skin aging including a method for inhibiting elastase and hyaluronidase has been found to be safe and efficient by using, for example, Chimaphila umbellata (L.) WPCBarton. The present invention has been completed which is extremely effective in the cosmetics field, pharmaceutical field, food field and the like.

The present invention is a plant belonging to the genus Chimaphila Pursh, for example, a food composition, a cosmetic composition, and a pharmaceutical composition containing, as an active ingredient, an extract of Chimaphila umbellata (L.) WPCBarton. And the like.
In addition, the present invention is a plant belonging to the genus Chimaphila Pursh, for example, skin aging prevention containing elastase inhibitor, hyaluronidase inhibitor, etc., which comprises chimophila umbellata (L.) WPCBarton as an active ingredient Provide the agent. The present invention also relates to a composition containing the inhibitor, including a food composition, a cosmetic composition, a pharmaceutical composition, and the like, and a composition utilizing the inhibitory effect of chimaphila umbellata (L.) WPCBarton and use thereof Provide usage in the field. For example, a skin anti-aging agent utilizing the inhibitory effect, a cosmetic composition containing the skin anti-aging agent, and an anti-aging method utilizing the skin anti-aging effect are also provided.

Furthermore, the present invention provides a method for producing an elastase inhibitor and a hyaluronidase inhibitor containing chimaphila umbellata (L.) WPCBarton as an active ingredient.
The prevention of skin aging according to the present invention includes at least the effect of preventing the formation of skin wrinkles and talmi, which are symbolic changes in appearance accompanying aging, and preventing the skin from being reduced in firmness and gloss. As a method to prevent skin aging, it contains an elastase inhibitor and hyaluronidase inhibitor, protects the skin from dryness, moisturizes to soften the hardened stratum corneum, and prevents stratum corneum stratification. Agents, cell activation components and turnover control components for reduced epidermal function, barrier function recovery agents for reduced barrier function, cell activators to prevent elastin and collagen producing fibroblasts, collagen Collagen synthesis promoters and collagenase activity inhibitors that suppress the activity of the degrading enzyme collagenase, blood circulation promoters that promote blood circulation, and around the basement membrane to prevent flattening of the dermal papillary layer due to aging Examples thereof include a method using a protective agent for the matrix component.

Examples of the plant belonging to the genus Chimaphila Pursh of the present invention include giant scorpion and sage.
The giant crape of the present invention is (PIPSISSEWA) Chimaphila umbellata (L.) WPCBarton, which is a perennial herb that grows naturally in a dry forest near the seashore. It originates in temperate zones such as North America, Europe and Siberia, and is distributed north of Ibaraki Prefecture in Japan. The giant buckwheat of the present invention has long been used as a fragrance for herbal tea and candy in the United States, and is a highly safe plant because it has been used for many years on humans.
Any of the whole grass or leaves, stems, roots, seeds and the like can be used for the giant grasshopper of the present invention. The Euglena can be used as it is, or, for example, after drying, it can be pulverized and used as a powder.

  In the method for extracting Euglena serrata according to the present invention, the above-mentioned Euglena can be extracted under conditions that do not lose the elastase inhibitory effect and / or the hyaluronidase inhibitory effect. For example, the extraction solvent includes water, lower alcohols such as methyl alcohol and ethyl alcohol, alcohols such as propylene glycol and polyhydric alcohols such as 1,3-butylene glycol, ketones such as acetone, diethyl ether, dioxane, Examples thereof include esters such as acetonitrile and ethyl acetate, xylene, benzene and chloroform. These solvents are extracted alone or using two or more mixed solvents. Moreover, when extracting again from an extraction residue, you may use not only the same solvent but another solvent. The ratio between the persica and the solvent of the present invention is not particularly limited, but it is 1 to 1,000 parts by weight of the solvent, particularly 5 to 100 in terms of extraction operation and efficiency, with respect to 1 part by weight of the persian grass. Part by weight is preferred. The extraction temperature is preferably in the range of room temperature to the boiling point of the solvent under normal pressure. As the extraction operation method, a general method can be employed. For example, an immersion method or a countercurrent extraction method may be used, and stirring may be used in combination as necessary. A solid can be separated from the extraction mixture to obtain an extract. The extract and the concentrate which are the Euglena extract of the present invention obtained by the above-mentioned process can be obtained. Furthermore, if necessary, a treatment such as partitioning or purification by various chromatography or decolorization with activated carbon may be added. As a deodorizing operation, an adsorption method using activated carbon, a steam distillation method, a deodorizing method by concentrating an aqueous solution, a supercritical fluid extraction method using carbon dioxide, a method of partitioning with an organic solvent, or the like can be employed. In addition, drying processes such as spray drying and freeze-drying, normal pressure, reduced pressure, concentration processes such as membranes, dilution, etc., may be arbitrarily adjusted as necessary according to the use of the extract of the perennial extract. Can do.

  The elastase inhibitor containing the Euglena extract of the present invention as an active ingredient only needs to contain at least the above-mentioned extract of Euphorbia, has the intended elastase inhibitory effect, and is a wrinkle caused by elastin injury due to elastase activity. It prevents, prevents or reduces skin elasticity such as talmi, skin aging, etc., and improves the commercial value of a cosmetic composition, for example. The elastase inhibitor of the present invention can be formulated into a solution or a powder form of the extract of Euphorbia or the most effective in the form of granules or paste according to the properties and processes of the product to be added. Can be processed into a dosage form that can be expected. In addition, the elastase inhibitor of the present invention may be in the form of a mixture of the above-mentioned extract of pertussis and other components. As such a component, one that does not impair the effect of the extract of persica and has a favorable effect on the processing and function of food composition, cosmetic composition, pharmaceutical composition, etc. should be used. Can do. Further, the Euglena spruce extract, which is an active ingredient of the elastase inhibitor of the present invention, may be used by calculating the addition amount and concentration from the intended effect and usage method.

  The hyaluronidase inhibitor containing the perennial extract of the present invention as an active ingredient only needs to contain at least the above-described extract of pertussis and has the intended hyaluronidase inhibitory effect, and skin aging and anti-inflammation caused by hyaluronidase activity Are prevented, prevented or reduced, and for example, the commercial value as a cosmetic composition is improved. The hyaluronidase inhibitor of the present invention can be formulated as a solution or a powder form of the extract of Psyllium or the most effective in the form of granules or paste according to the properties and processes of the product to be added. Can be processed into a dosage form that can be expected. In addition, the hyaluronidase inhibitor of the present invention may be in the form of a mixture of the above-mentioned extract of pertussis and other components. As such a component, one that does not impair the effect of the extract of persica and has a favorable effect on the processing and function of food composition, cosmetic composition, pharmaceutical composition, etc. should be used. Can do. Furthermore, the extract of Euglena serrata, which is an active ingredient of the hyaluronidase inhibitor of the present invention, may be used by calculating the addition amount and concentration from the intended effect and usage method.

  The cosmetic composition of the present invention is a cosmetic composition (including quasi-drugs) containing the giant megakosa of the present invention as an active ingredient. Furthermore, it is a cosmetic composition that has Euphorbia as an active ingredient and exhibits an elastase inhibitory effect and / or a hyaluronidase inhibitory effect. The dosage form of the cosmetic composition of the present invention is arbitrary, and cosmetics such as capsules, granules, powders, solids, liquids, gels, bubbles, emulsions, creams, ointments, sheets, etc. It can be used in combination with quasi drugs and cosmetics for skin and hair. Specifically, for example, external pharmaceutical preparations, lotions, creams, ointments, emulsions, lotions, oils, packs, and other basic cosmetics, facial cleansers and skin cleansing agents, massage agents, cleansing agents, aftershave lotions, pre-shows Hair lotion, shaving cream, shampoo, rinse, hair treatment, hair conditioner, perm, hair nick, hair dye, hair growth / hair restoration, etc., foundation, lipstick, blusher, eye shadow, eyeliner, mascara It can be applied to various products such as make-up cosmetics, perfumes, bath preparations, other dentifrices, mouth fresheners, deodorants / preservatives, sanitary cotton, and wet tissues.

  The method for producing the cosmetic composition of the present invention includes oils and fats such as vegetable oils, waxes such as lanolin and beeswax, plant extracts such as button pi, yellow aloe vera and senna, hydrocarbons, fatty acids, higher alcohols, esters, Various surfactants, pigments, fragrances, vitamins, animal extracts, UV absorbers, antioxidants, antiseptics, bactericides, and other ingredients that are used as normal cosmetic ingredients are appropriately blended and manufactured. The effect can also be enhanced by using in combination with other plant extracts generally known as elastase inhibitors and / or hyaluronidase inhibitors. It is preferable to determine the blending amount of the giant buckwheat of the present invention in consideration of its dosage form, etc., but generally 0.001 to 50% by weight, preferably 0.01 to 20% by weight based on the total amount It should be about.

  The food composition of the present invention is a food composition containing the giant megakosa of the present invention as an active ingredient. Further, the present invention provides a food composition that has Euphorbia as an active ingredient and exhibits an elastase inhibitory effect and / or a hyaluronidase inhibitory effect. Various kinds of processed foods can be mentioned, and foods that can be eaten as they are are preferred. For example, various foods and drinks such as liquid food, solid food, powder, extract extract, paste, and dried food are included. As typical examples, as various beverages, vegetable beverages, fruit beverages, milk beverages, alcoholic beverages, etc., as various confectionery items, cookies, biscuits, chocolate, strawberries, chewing gum, candy, jelly, etc., as various dairy products, Yogurt, cheese and the like can be exemplified as various frozen desserts, ice cream and the like, and various livestock products can be exemplified by ham and sausage. Furthermore, various seasonings, soup, curry, noodles, breads, pickles, cereals, tofu, sprinkles, drinks and the like can be exemplified. However, the above examples are not intended to limit the elastase inhibitory effect and / or the form of the hyaluronidase inhibitor of the present invention.

  The method for producing a food composition of the present invention can be performed by any known method. As the form of the food containing the persica as the active ingredient of the present invention, in addition to the normal food form, tablet food such as capsules, concentrated liquid food, natural liquid food, semi-digested nutrition food, ingredient nutrition food, drink nutrition food Oral enteral nutritional foods, functional foods and the like. The food composition of the present invention further includes glucose, fructose, sucrose, maltose, sorbitol, stevia extract, licorice extract, corn syrup, lactose, citric acid, tartaric acid, malic acid, succinic acid, lactic acid, L-ascorbine Acid, dl-α-tocophenol, sodium erythorbate, glycerin, propylene glycol, glycerin fatty acid ester, polyglycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester, propylene glycol fatty acid ester, gum arabic, carrageenan, casein, gelatin, Pectin, agar, vitamin Bs, calcium pantothenate, amino acids, calcium salts, pigments, fragrances, preservatives, etc. can be prepared by appropriately blending those used as usual food ingredients, and more generally Elasta By peptidase inhibitory effect and / or hyaluronidase inhibitory effect in combination such as other plant extracts known, it is also possible to enhance the effect. It is preferable to determine the blending amount of the giant buckwheat of the present invention in consideration of its dosage form, etc., but generally 0.001 to 50% by weight, preferably 0.01 to 20% by weight based on the total amount It should be about.

  The method using the enzyme inhibitory effect having the elastase inhibitory effect and / or the hyaluronidase inhibitory effect comprising the giant red snapper as an active ingredient is a composition and method containing the giant red snapper, for example, a composition containing a protein or a cell. , Food compositions, cosmetic compositions, and pharmaceutical compositions, and any method that exhibits the enzyme inhibitory effect is not limited thereto. The above-mentioned giant megasas which is an active ingredient of the present invention has hyaluronidase and elastase inhibitory activity, and thus is excellent in the effect of retaining skin elasticity and elasticity. In addition, the superior hyaluronidase inhibitory action is effective in preventing and treating inflammation and allergies caused by hyaluronidase. The field of application can be used in various external preparations including animal preparations, and specifically, ampoules, capsules, pills, tablets, powders, granules, solids, liquids Gels or foams 1) Pharmaceuticals, 2) Quasi-drugs, 3) Topical or systemic skin cosmetics (eg lotions, emulsions, creams, ointments, lotions, oils, packs, etc.) Basic cosmetics, facial cleansers and skin cleansers, massage agents, cleansing agents, depilators, depilators, shaving treatments, after shave lotion, pre-show broth, shaving cream, etc.) 4) Applicable to scalp and hair Medicinal or / and cosmetic preparations (for example, shampoos, rinses, treatments, permanents, hair dyes, hair styling, hair stylings, hair growth / hair growth 5) Bathing agents used in bath water, 6) In addition, deodorizing / deodorizing agents, sanitary products, sanitary cotton, wet tissue, and the like. Moreover, the utilization to a general food composition is also possible.

The above-mentioned Euglena as an active ingredient of the present invention has hyaluronidase and elastase inhibitory activity, so that it is excellent in the effect of retaining skin elasticity and elasticity, and is safe from plants. In addition, it has an excellent inhibitory action on hyaluronidase, and is effective in preventing and treating inflammation and allergies caused by hyaluronidase, and can be applied to food compositions, pharmaceutical compositions, and various cosmetic compositions in the cosmetic field.
In addition, the plant belonging to the genus Umegasausaceae to which the Euphorbia genus of the present invention belongs has superior elastase inhibitory action and the like, and has a higher skin aging prevention effect than the plants of the genus Ichyakusaceae to which Ichiakuso belongs.
EXAMPLES Hereinafter, although an Example demonstrates this invention concretely, this invention is not limited by a following example.

  Purified water (1,000 ml) was added to 100 g of persian grass and extracted by immersion for 30 minutes at room temperature, followed by suction filtration to obtain an extract of Scotsweed. This was concentrated to dryness under reduced pressure, and heated and dried at 60 ° C. for 12 hours or longer to obtain 11.5 g of a powder of the extract of persica.

  After adding 1,000 ml of 50% ethyl alcohol to 100 g of the whole grass, it was subjected to immersion extraction at room temperature for 1 hour, followed by suction filtration to obtain an extract of Euphorbia. This was concentrated to dryness under reduced pressure, and heated and dried at 60 ° C. for 12 hours or longer to obtain 12.1 g of Cyprus extract.

  After adding 1,000 ml of 50% ethyl alcohol to 100 g of persian leaves, it was subjected to immersion extraction at room temperature for 4 hours, followed by suction filtration to obtain an extract of persica. This was concentrated to dryness under reduced pressure, and heated and dried at 60 ° C. for 12 hours or longer to obtain 13.4 g of a powder of the extract of Cyprus persica.

<Measurement of elastase inhibitory activity>
The elastase inhibitory action was evaluated using porcine pancreatic elastase (manufactured by Wako Pure Chemical Industries) and the synthetic substrate succinyl-L-alanyl-L-alanyl-L-alanine-p-nitroanilide (manufactured by Nacalai Tesque). The substrate was adjusted to 0.1 M with dimethyl sulfoxide, and a 1 mM solution diluted 100-fold with 0.2 M Tris-HCl buffer (pH 8.8) was used at the time of use. The enzyme was adjusted to 0.05 unit / ml with 0.2 M Tris-HCl buffer (pH 8.8). 100 μl of substrate was reacted with 50 μl of enzyme and 50 μl of sample at 37 ° C. for 30 minutes, and then the absorbance was measured at 405 nm. Samples obtained in Examples 2 and 3 were prepared with a 0.2M Tris-HCl buffer (pH 8.8) to a concentration of 0.1% and used. The same enzyme reaction and absorbance measurement as described above were performed by adding a buffer solution instead of the sample solution. Further, in each case, the same operation and measurement were performed by adding a buffer solution without adding an enzyme. The inhibition rate was calculated by the following formula 1, and the above measurement results are shown in Table 1. As controls, yew extract, which is known to have inhibitory activity against elastase (produced by the following preparation method), loquat leaf extract (commercial product), hamamelis extract (commercial product), peonies extract (commercial product) ) Was used in the test.
For Pyrola japonica klenze ex Alef, add 1,000 ml of 50% ethanol to 100 g of whole plant, soak and extract at room temperature for 1 hour, and then suction filter to obtain the extract of Ichyakus It was. This was concentrated to dryness under reduced pressure, and dried by heating at 60 ° C. for 12 hours or more to obtain a powder of Ichiyaku extract.

この結果、本発明のオオウメガサソウ抽出物は、ビワ葉抽出物、ハマメリス抽出物、シャクヤク抽出物と比較してエラスターゼ活性を有意に阻害する作用を有することが示された。また、本発明のオオウメガサソウが属するイチヤクソウ科ウメガサソウ属の植物は、イチヤクソウが属するイチヤクソウ科イチヤクソウ属の植物と比較して、エラスターゼ活性を有意に阻害する作用を有することが示された。特にオオウメガサソウの葉からの抽出物は、他の部位に比べエラスターゼ活性を有意に阻害する作用を有することが示された。よって、本発明のオオウメガサソウは、皮膚のハリ、弾力を回復・維持することにより皮膚の老化を防止する効果を奏することが示唆された。

As a result, it was shown that the Euglena spruce extract of the present invention has an action of significantly inhibiting elastase activity as compared with the loquat leaf extract, the hamamelis extract, and the peony extract. Further, it was shown that the plant belonging to the genus Umegasausae belonging to the genus Euphorbia belongs to the present invention has an action of significantly inhibiting elastase activity as compared with the plants belonging to the genus Ichasaceae belonging to the genus Ichaxaceae. In particular, it was shown that the extract from the leaves of Euphorbia has an effect of significantly inhibiting elastase activity compared to other sites. Therefore, it was suggested that the giant grasshopper of the present invention has an effect of preventing skin aging by restoring and maintaining the elasticity and elasticity of the skin.

<Measurement of hyaluronidase inhibitory activity>
Evaluation of the hyaluronidase inhibitory action was performed by the following method.
Add 200 μl of sample to 100 μl of hyaluronidase (type IV-S from Bovine testis, manufactured by Sigma) solution (4,500 units / ml; dissolved in 0.1 M acetate buffer (pH 4.0)) and leave at 37 ° C. for 20 minutes. did. Next, 200 μl of enzyme activator (Compound 48/80, Sigma) solution (0.5 mg / ml; dissolved in 0.1 M acetate buffer (pH 4.0)) was added, and the mixture was allowed to stand at 37 ° C. for 20 minutes. 500 μl of a substrate potassium hyaluronate (from rooster comb, manufactured by Wako Pure Chemical Industries, Ltd.) solution (0.8 mg / ml; dissolved in 0.1 M acetic acid buffer (pH 4.0)) was added and left at 37 ° C. for 40 minutes. did. Next, 200 μl of 0.4N sodium hydroxide solution was added to stop the reaction, 0.2 ml of 0.8M boric acid solution was added, and the mixture was heated in boiling water for 3 minutes. After cooling to room temperature, 6 ml of 1% p-dimethylaminobenzaldehyde acetic acid solution was added, and the mixture was allowed to stand at 37 ° C. for 20 minutes, and then the absorbance at 585 nm was measured. The sample obtained in Example 2 was adjusted to a concentration of 0.2% with 0.1 M acetate buffer (pH 4.0). The same enzyme reaction and absorbance measurement as described above were performed by adding a buffer solution instead of the sample solution. Further, in each case, the same operation and measurement were performed by adding a buffer solution without adding an enzyme. The inhibition rate was calculated by the following formula 2, and the above measurement results are shown in Table 2.

この結果、本発明のオオウメガサソウ抽出物は、オウゴン抽出物、ラカンカ抽出物、緑茶抽出物と比較して、ヒアルロニダーゼ活性を有意に阻害する作用を有することが示され、皮膚のハリ、弾力を回復・維持することにより皮膚の老化を防止し、さらに抗炎症、抗アレルギー作用を有することが示唆された。

As a result, it was shown that the Euglena spp. Extract of the present invention has an action of significantly inhibiting hyaluronidase activity compared with the extract of Ogon, Lacanca, and green tea, and restores the elasticity and elasticity of the skin.・ It was suggested that it prevents skin aging by maintaining it, and also has anti-inflammatory and anti-allergic effects.

製法：精製水にオレイルアルコール、オオウメガサソウ抽出物を室温にて溶解し水相とした。エチルアルコールに香料、グリセリン、ポリオキシエチレン（２０）ソルビタンモノラウリン酸エステルを溶解し、先の水相に混合した。
１０名のパネルを対象に、上記化粧水を１回/日、２ヶ月間使用したところ、内７名が乾燥肌、肌荒れが改善され、皮膚のハリや弾力を回復した。
また、本実施例のオオウメガサソウ抽出物(実施例２)の代わりに、実施例１又は実施例３のオオウメガサソウ抽出物を使用した以外は同一の方法で製造した各化粧水について、本実施例とはパネル対象者を変更し、本実施例と同一方法でパネル試験を行った結果、本実施例のパネル試験結果と同様に乾燥肌、肌荒れが改善され、皮膚のハリや弾力回復の効果を奏した。 (Lotion)

Production method: Oleyl alcohol and Euphorbia extract were dissolved in purified water at room temperature to obtain an aqueous phase. A perfume, glycerin, and polyoxyethylene (20) sorbitan monolaurate ester were dissolved in ethyl alcohol and mixed with the previous aqueous phase.
When the above lotion was used once a day for 2 months for 10 panelists, 7 of them improved dry skin and rough skin and recovered skin firmness and elasticity.
In addition, each lotion produced in the same manner as in Example 1 or Example 3 except that the perennial extract of Example 1 or Example 3 was used in place of the extract of Example 2 (Example 2). As a result of changing the panel subject and performing the panel test in the same manner as in this example, dry skin and rough skin were improved as in the panel test result of this example, and the effect of restoring skin elasticity and elasticity was improved. I played.

製法：精製水にオオウメガサソウ抽出物を溶解した（水相）。エチルアルコールにそれ以外のものを溶解した（アルコール相）。アルコール相に水相を添加し、濾紙などで濾過した。
１０名のパネルを対象に、上記ヘアリキッドを１回/日、３ヶ月間使用したところ、内６名の頭皮のかゆみが改善された。
また、本実施例のオオウメガサソウ抽出物(実施例１)の代わりに、実施例２又は実施例３のオオウメガサソウ抽出物を使用した以外は同一の方法で製造した各ヘアリキッドについて、本実施例とはパネル対象者を変更し、本実施例と同一方法でパネル試験を行った結果、本実施例のパネル試験結果と同様に頭皮のかゆみ改善効果を奏した。 (Hair liquid)

Production method: Persian extract was dissolved in purified water (aqueous phase). Others were dissolved in ethyl alcohol (alcohol phase). The aqueous phase was added to the alcohol phase and filtered with filter paper or the like.
When the above-mentioned hair liquid was used once per day for 3 months for 10 panels, itching of the scalp of 6 persons improved.
Moreover, about each hair liquid manufactured by the same method except using the perennial extract of Example 2 or Example 3 instead of the perennial extract of Example (Example 1), this Example As a result of changing the panel subject and performing the panel test in the same manner as in this example, the effect of improving scalp itchiness was obtained in the same manner as the panel test result in this example.

製法：砂糖、水飴、水を混合し、温度１５０℃で煮詰めた。煮詰後１２０℃まで冷却し、香料、クエン酸、オオウメガサソウ抽出物を加え混合し、成形した。
風邪をひいて喉の痛みを生じている４名のパネルを対象に、上記キャンディを飲食したとこを、内３名が喉の痛みが無くなるか、軽減された。
また、本実施例のオオウメガサソウ抽出物(実施例１)の代わりに、実施例２又は実施例３のオオウメガサソウ抽出物を使用した以外は同一の方法で製造した各キャンディーについて、本実施例とはパネル対象者を変更し、本実施例と同一方法でパネル試験を行った結果、本実施例のパネル試験結果と同様に喉の痛みが無くなるか、軽減する効果を奏した。 (candy)

Production method: Sugar, starch syrup, and water were mixed and boiled at a temperature of 150 ° C. After boiling, the mixture was cooled to 120 ° C., added with fragrance, citric acid and persica extract, mixed and molded.
Of the four panelists who suffered from a cold and caused sore throat, eating and drinking the above candy, three of them disappeared or were alleviated.
In addition, each candy produced by the same method as in Example 2 or 3 except that it was used in place of the perennial extract of Example (Example 1). Changed the panel subject, and as a result of performing the panel test by the same method as the present example, as in the panel test result of the present example, there was an effect of eliminating or reducing the sore throat.

Claims (10)

    Skin aging inhibitor containing as an active ingredient a plant belonging to the genus Chimaphila Pursh.   The skin aging inhibitor according to claim 1, wherein the plant to which Chimaphila Pursh belongs is Chimaphila umbellata (L.) W.P.C.Barton.   An elastase inhibitor containing chimaphila umbellata (L.) W.P.C.Barton as an active ingredient.   A hyaluronidase inhibitor containing chimaphila umbellata (L.) W.P.C.Barton as an active ingredient.   A cosmetic composition comprising an elastase inhibitor and / or a hyaluronidase inhibitor, which comprises chimaphila umbellata (L.) W.P.C.Barton as an active ingredient.   A food composition containing chimaphila umbellata (L.) W.P.C.Barton as an active ingredient.   A food composition comprising an elastase inhibitor and / or a hyaluronidase inhibitor, which comprises chimaphila umbellata (L.) W.P.C.Barton as an active ingredient.   A method for producing an elastase inhibitor and / or a hyaluronidase inhibitor, which comprises chimaphila umbellata (L.) W.P.C.Barton as an active ingredient.   A composition using an elastase inhibitory effect and / or a hyaluronidase inhibitory effect comprising chimaphila umbellata (L.) W.P.C.Barton as an active ingredient.   A method using elastase inhibitory effect and / or hyaluronidase inhibitory effect, which contains chimaphila umbellata (L.) W.P.C.Barton as an active ingredient