JP2003301288A - Electrolytically reduced water containing colloid and manufacturing method therefor - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a stable electrolytically reduced water containing colloids, which has strong antioxidant properties, is utilized for treating or preventing cancer or diabetes, and prevents bacteria from proliferating, and to provide a manufacturing method therefor. <P>SOLUTION: The electrolytically reduced water containing the colloids includes the colloids of a hydrogen absorbing metal as a carrier of active hydrogen. The method for manufacturing the electrolytically reduced water containing the colloids comprises a step of adding the colloids of the hydrogen absorbing metal to an aqueous electrolytic solution, a step of introducing the solution to a cathode chamber and an anode chamber which are separated by a diaphragm, a step of electrolyzing the solution, and a step of taking out the electrolytically reduced water formed in the cathode chamber. Alternatively, the method for manufacturing the electrolytically reduced water containing the colloids comprises a step of introducing the aqueous electrolytic solution to the cathode chamber and the anode chamber, a step of electrolyzing the solution, a step of taking out the cathode water formed in the cathode chamber, and a step of adding the colloids of the hydrogen absorbing metal to the cathode water. <P>COPYRIGHT: (C)2004,JPO

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to an active oxygen scavenging action and a free radical scavenging action.
It is called "antioxidant". ) Large and stable colloid-containing electrolytically reduced water and a method for producing the same.

[0002]

2. Description of the Related Art When water in which an electrolyte such as NaOH is dissolved is introduced into a cathode chamber and an anode chamber separated by a diaphragm and electrolyzed, active hydrogen, which is highly reactive atomic hydrogen, is generated in the cathode chamber. To do. The water rich in active hydrogen generated in the cathode chamber is generally called electrolyzed reduced water (cathode water). Electrolyzed reduced water is useful for the prevention and treatment of various diseases, suppresses the progress of aging, and is used in fields other than medicine. However, it is expected to be used in many fields such as food preservation and semiconductor cleaning. Such an effect of the electrolytically reduced water is due to the antioxidative action caused by the reducing property (antioxidant property) of the active hydrogen in the electrolytically reduced water. Active oxygen means that oxygen molecule has one electron
Pieces containing (O ₂ ·-), hydroxyl group (· OH),
It refers to hydrogen peroxide (H ₂ O ₂ ), singlet oxygen ( ¹ O ₂ ), etc. The active oxygen eliminates the active oxygen by the following mechanism, and is called a reducing action and an antioxidant action.

The action of eliminating active oxygen is represented by the following reaction formula. O ₂ · − + 2H · → H ₂ O ₂ + e ⁻ · OH + H · → H ₂ O H ₂ O ₂ + 2H · → 2H ₂ O The free radical scavenging action is represented by the following reaction formula.

R. + H..fwdarw.RH Although the electrolytic reduced water is expected to have such an effect, the electrolytic reduced water having a strong antioxidant action could not be produced by the conventional technique. Therefore, when it is used for treating cancer or diabetes, for example, the effect cannot be obtained unless a large amount of electrolytically reduced water is drunk for a long period of time. In addition, active hydrogen itself is extremely unstable, and immediately becomes hydrogen ions and hydrogen gas. Therefore, the time during which active hydrogen exists as a simple substance is extremely short, and the antioxidant action of electrolytically reduced water is unstable.

[0005]

SUMMARY OF THE INVENTION It is an object of the present invention to provide a colloid-containing electrolytically reduced water having a strong antioxidative action and stable, and a method for producing the same.

[0006]

The colloid-containing electrolytic reduced water of the present invention is characterized in that the electrolytic reduced water contains a hydrogen storage metal colloid as a carrier of active hydrogen. As the hydrogen storage metal colloid, a platinum colloid, a palladium colloid or a vanadium colloid is preferable.

In the method for producing colloid-containing electrolytically reduced water of the present invention, a step of adding a hydrogen storage metal colloid to an aqueous solution containing an electrolyte and introducing the aqueous solution into each of a cathode chamber and an anode chamber separated by a diaphragm. Step, the cathode is immersed in the cathode chamber, while the anode is immersed in the anode chamber, by energizing between the cathode and the anode, the step of electrolyzing the aqueous solution, electrolytic reduced water obtained in the cathode chamber And the process of taking out.

Further, the method for producing colloid-containing electrolytically reduced water of the present invention comprises the steps of introducing an aqueous solution containing an electrolyte into each of the cathode chamber and the anode chamber separated by a diaphragm, and immersing the cathode in the cathode chamber to form an anode. In a state where the anode is immersed in the chamber, a step of electrolyzing the aqueous solution by energizing between the cathode and the anode, a step of extracting the cathode water obtained in the cathode chamber, and a colloid of a hydrogen storage metal in the cathode water. Is added.

Platinum colloid is hexachloroplatinic acid (H ₂
PtCl ₆ ) reduces the palladium colloid by reducing palladium chloride (PdCl ₂ ), and the vanadium colloid changes by vanadium chloride (VC).
It is preferably obtained by reducing l ₂ ).

[0010]

BEST MODE FOR CARRYING OUT THE INVENTION (Colloid-containing electrolytic reduced water) The colloid-containing electrolytic reduced water of the present invention is characterized in that the electrolytic reduced water contains a hydrogen storage metal colloid as a carrier of active hydrogen. Active hydrogen in electrolytically reduced water is unstable as a simple substance, and immediately becomes hydrogen ions and hydrogen gas, and loses its antioxidant effect. However, when the electrolyzed reduced water contains a hydrogen storage metal colloid, the active hydrogen is taken into the hydrogen storage metal colloid and stably exists in the active hydrogen state. Also, because active hydrogen is the smallest atom,
It is known to be adsorbed and stored on almost all metals and metal colloids. Therefore, the hydrogen storage metal colloid functions as a carrier of active hydrogen, and the electrolytic reduction water can be stably maintained in a high antioxidative state. In addition, the nanometer-scale hydrogen storage metal colloid has a large proportion of surface atoms compared to metal pieces and powdery metal, and therefore has a large active hydrogen adsorption and storage capacity, and forms a uniform dispersion and precipitates. The tendency is small, so it is suitable for drinking. In addition, when used in a colloidal state, a large amount of metal can be blended, and the concentration can be easily controlled as compared with the case where the metal is dissolved and used.

The hydrogen storage metal colloid of the present invention is a so-called dispersed colloid in which fine particles of the hydrogen storage metal are dispersed in water. The hydrogen storage metal is a metal that easily absorbs and stores hydrogen between lattices of metal crystals to form a metal hydride. Hydrogen storage metals include platinum, palladium, vanadium, magnesium, zirconium, and the like, as well as hydrogen storage alloys. As a hydrogen storage alloy, Mg ₂ N
i, LaNi ₅ , TiFe and the like. As the hydrogen storage metal, platinum, palladium or vanadium is preferable because it can easily store active hydrogen in the electrolytically reduced water and can stably retain the active hydrogen.

Size of fine metal particles dispersed as a hydrogen storage metal colloid (particle diameter or side length)
Is preferably 0.3 nm to 1 μm in terms of a large proportion of surface atoms, good antioxidative effect and dispersibility, and 0.3
nm to 3 nm is more preferable.

The colloid-containing electrolytically reduced water contains chlorine (C
It is preferable that the content of l ₂ ) and hypochlorous acid (HClO) is small. This is because chlorine and hypochlorous acid have a harmful effect on the living body and reduce the useful physiological effect of electrolytically reduced water containing colloid. Specifically, chlorine and hypochlorous acid are preferably less than 0.1 mg / l, more preferably 0.08 mg / l or less, and particularly preferably 0.0
It is 1 mg / l or less.

Electrolytically reduced water easily absorbs active hydrogen,
Na, K, and C can be stably held in the state of active hydrogen.
Colloids of a, Mg, Fe, Ag, Cu or the like, or those containing these ions, platinum ion, palladium ion or vanadium ion are preferable. The concentration of the metal ion or metal colloid in the electrolytically reduced water of the present invention is, for example, 1 mg / l to 500 mg / l, preferably 5 mg / l.
mg / l to 200 mg / l, more preferably 10
It is mg / l to 100 mg / l.

The content of other inorganic substances, heavy metals and general organic chemical substances in the colloid-containing electrolytically reduced water preferably satisfies the standards for tap water in Japan. For example, cadmium 0.01 mg / l or less, mercury 0.0005 mg / l or less, benzene 0.01 mg / l
It is preferably 1 or less, and total trihalomethane is 0.1 mg / l or less.

The redox potential of the colloid-containing electrolytically reduced water is 12 ° C to 1 because it has a large ability to occlude active hydrogen.
At 4 ° C, it is preferably -5 to -1000 mV, more preferably -20 to -1000 mV, and particularly preferably -50 mV to -1000 mV. The redox potential can be measured by a redox potential meter (manufactured by Toa Denpa Kogyo).

The pH of the colloid-containing electrolytically reduced water is 12 ° C. or higher in that it is drunk and the stability of active hydrogen is increased.
7-11 is preferable at 14 degreeC, More preferably, it is 8
~ 11. The pH adjustment can use a buffer such as sodium phosphate or acidic water obtained at the anode in electrolysis.

Active hydrogen in the electrolytically reduced water containing colloid is
In terms of securing sufficient antioxidative effect and exerting anticancer (anticancer) effect, antibacterial effect, or oxidative stress suppressing effect,
0.01 μM to 10 μM (μM at 12 ° C. to 14 ° C.)
Is preferably μmol / l), more preferably 0.1 μM to 10 μM, particularly preferably 1 μM to 10 μM.
It is M. The active hydrogen present in the colloid-containing electrolytically reduced water was quantified by using a coloring reaction in which a sodium salt of 3,5-dibromo-4-nitrosobenzenesulfonic acid (DBNBS) reacts with active hydrogen to produce a DBNBS azo compound. Can be done. That is, since the DBNBS azo compound has an absorption peak at a wavelength of 450 nm,
The amount of active hydrogen can be calculated from the absorbance.

(Method for producing colloid-containing electrolytic reduced water) The method for producing colloid-containing electrolytic reduced water according to the present invention comprises a step of adding a hydrogen storage metal colloid to an aqueous electrolyte solution, and a cathode chamber and an anode chamber separated by a diaphragm. Of the aqueous solution, the step of electrolyzing the aqueous solution, and the step of taking out the electrolytically reduced water obtained in the cathode chamber. Further, the method for producing colloid-containing electrolytic reduced water of the present invention, a step of introducing an electrolyte aqueous solution into the cathode chamber and the anode chamber, a step of electrolyzing the aqueous solution, a step of extracting the cathode water obtained in the cathode chamber, And a step of adding a hydrogen storage metal colloid to the cathode water.

FIG. 1 is a conceptual diagram of an electrolytic cell 10 used in the present invention. The electrolytic cell 10 includes a cathode chamber 12 including a cathode 11.
And an anode chamber 14 including an anode 13. The cathode chamber 12 and the anode chamber 14 are separated by a diaphragm 15. A cathode water extraction pipe 16 for extracting cathode water (electrolytically reduced water) obtained by electrolysis is connected to the cathode chamber 12, and anode water (acidic water) obtained by electrolysis is connected to the anode chamber 14.
An anode water drain pipe 17 for taking out is connected. Cathode chamber 1
A water supply pipe 18 is connected to each of the anode 2 and the anode chamber 14.

Before electrolysis, an aqueous solution containing an electrolyte is introduced into each of the cathode chamber and the anode chamber through a water supply pipe. The electrolyte refers to a substance that exhibits ion conductivity when dissolved in water, and examples thereof include NaOH and NaCl.

As water for dissolving the electrolyte, a specific resistance of 10
While × can be used 10 ⁴ Ω · cm or more pure water, it is preferable to use ultrapure water which is the resistivity 100 × 10 ⁴ Ω · cm or more. Pure water can be produced by an ion-exchange desalting method using a hydrogen-type strongly acidic cation exchange resin and a hydroxide-type strongly basic exchange resin. Ultrapure water can be produced by a mixed bed polisher in which a strongly acidic cation exchange resin and a strongly basic anion exchange resin are mixed.

In the step of electrolyzing an aqueous solution containing an electrolyte, cathode water (electrolytically reduced water) is generated in the cathode chamber and anode water (acidic water) is generated in the anode chamber. When the aqueous solution of the electrolyte is electrolyzed, hydrogen ions (H ⁺ ) gather in the cathode chamber, and the hydrogen ions combine with the electrons (e ⁻ ) supplied from the cathode,
It becomes active hydrogen (atomic hydrogen H.). Since active hydrogen has high reactivity, two active hydrogens combine to form hydrogen gas (H ₂ ) depending on the conditions of electrolysis. The reason why the electrolytically-reduced water has the effect of suppressing the growth of cancer and the effect of suppressing metastasis is that active hydrogen in the electrolytically-reduced water exerts an antioxidant effect. Therefore, in order to enhance the antioxidant effect, it is necessary to dissolve a large amount in water in a state before becoming hydrogen gas, that is, in a state of active hydrogen.

The device used for electrolysis is preferably a device in which electrolysis cells are connected in series or a device in which electrolytically reduced water repeatedly passes through the electrolysis cell in order to increase the concentration of active hydrogen. An apparatus (TI-7000S manufactured by Nippon Trim) in which two electrolytic cells are connected in series to FIG. 2 (a), FIG. 2 (b)
A device in which three electrolytic cells are connected in series to each other (TI
-7000 3S), FIG. 2C shows a device (circulation electrolytic reduction device) in which electrolytically reduced water is circulated and passes through three electrolytic cells many times.

For example, in the device shown in FIG. 2A in which two electrolysis cells are connected in series, the cathode water (electrolytically reduced water) obtained by electrolysis in the first electrolysis cell 1 is first electrolyzed. It is introduced from the cathode chamber 24 of the cell 1 into the cathode chamber 29 of the second electrolytic cell 2 and is further electrolyzed. Therefore, cathode water (electrolytically reduced water) having a high concentration of active hydrogen can be produced. The same applies to an apparatus in which three or more electrolytic cells are connected in series. Also in the device shown in FIG. 2C in which the cathode water (electrolytically reduced water) is allowed to pass through the three electrolytic cells many times, the cathode chambers of the electrolytic cells are connected to each other and the anode chambers are connected to each other. However, after passing through the third electrolyzer 3, it is circulated many times in one system by adjusting the cock provided in the pipe without being drained immediately. High cathode water (electrolytically reduced water) can be produced. FIG. 2 (c) shows an example in which three electrolysis cells are connected, but when two electrolysis cells are connected, 4
The same applies when two or more electrolytic cells are connected. After electrolysis, take out cathode water (electrolytically reduced water) from the cathode chamber.

In the method for producing electrolytically reduced water containing colloid of the present invention, the hydrogen storage metal colloid can be added to the aqueous electrolyte solution before electrolysis. It can also be added to the cathode water after electrolysis. When the hydrogen storage metal colloid is added to the electrolyte aqueous solution before electrolysis, active hydrogen having a short life generated by electrolysis can be immediately incorporated into the hydrogen storage metal colloid and stabilized. Further, the work efficiency can be improved by adding a colloid of a hydrogen storage metal to the cathode water after electrolysis.

Among the hydrogen storage metal colloids, platinum colloids can form uniform and fine colloids, and active hydrogen (H.) can be stably retained on the surface of the platinum colloids. ₂ PtCl ₆ ) is preferably produced. For example, a method of applying an external flame of Bunsen burner to the surface of the aqueous solution of hexachloroplatinic acid for reduction and a method of reducing the aqueous solution of hexachloroplatinic acid with hydrazine or ethanol are preferable. Alternatively, the platinum colloid can be produced by a method of blowing an arc between two platinum wires immersed in pure water.

Among the hydrogen storage metal colloids, the palladium colloid is palladium chloride (PdCl ₂ ) because it can store a large amount of hydrogen in the palladium colloid.
Is preferably produced by a method of reducing. For example, a method of reducing an aqueous solution of palladium chloride with sodium formate and sodium carbonate and a method of blowing (bubbling) hydrogen gas (H ₂ ) are preferable.

Among the hydrogen storage metal colloids, the vanadium colloid is preferably produced by a method of reducing vanadium chloride (VCl ₂ ) because it can store a large amount of hydrogen in the vanadium colloid. For example, a method of reducing an aqueous solution of vanadium chloride with sodium formate and sodium carbonate and a method of blowing (bubbling) hydrogen gas (H ₂ ) are preferable.

The hydrogen storage metal colloid is preferably an aqueous dispersion from the viewpoint of enhancing the compatibility. The amount of hydrogen storage metal colloid added is 0.01 to the total electrolyte aqueous solution.
1000 ppm is preferable, 0.1-500 ppm is more preferable, 1-300 ppm is particularly preferable. If the amount of the hydrogen storage metal colloid added is less than 0.01 ppm, the amount of active hydrogen that can be stored is small, and a sufficient antioxidant effect cannot be obtained. On the other hand, if the amount of the hydrogen storage metal colloid added exceeds 1000 ppm, it becomes difficult to stably retain the fine colloid.

Example 1 86 ml of ultrapure water was placed in a closed container and placed in a water bath for 6 minutes.
The temperature was raised to 0 ° C. 99% ethanol as reducing agent 10
ml, and a surfactant (Tw manufactured by Wako Pure Chemical Industries, Ltd.
een80) 100 μl was added, and while continuing stirring, 7
The temperature was raised to 0 ° C., 106 mg of hexachloroplatinic acid was added, 0.2 g of NaHCO ₃ was dissolved in ultrapure water, and 4 ml was added.
Was mixed as a buffer. The temperature was maintained at 70 ° C., and when hexachloroplatinic acid was reduced to platinum and turned black, heating and stirring were stopped. After allowing to stand for 12 hours, add 500 ml of ultrapure water and cut off the molecular weight to 100.
Ultrafiltration was performed using a No. 00 filter membrane, and washing and concentration were performed.

After ultrafiltration, 50 ml of ultrapure water was added. When observed with a transmission electron microscope, as shown in FIG.
Many fine particles of 1 nm to 5 nm were recognized. It was confirmed by electron microscopic analysis that these fine particles were platinum colloids. In addition, high performance liquid chromatography (HPL
When measured in C), there was an absorption peak at a wavelength of 220 nm. Hexachloroplatinic acid ions have no absorption at 220 nm, but when hexachloroplatinic acid is reduced to platinum colloid, it absorbs at 220 nm. Therefore, the fine particles shown in FIG. 6 are platinum colloids. It could be confirmed.

An electrolyte (NaOH) was dissolved in ultrapure water to obtain a 5 mM NaOH aqueous solution. This aqueous solution 100
The platinum colloid described above was added to 0 ml so as to be 2 ppm. The cathode water shown in FIG. 2 (c) was placed in an apparatus (circulation electrolytic reduction apparatus) that allowed it to pass through three electrolysis cells many times, and electrolyzed at 0.13 A / cm ² for 60 minutes to remove it from the cathode chamber. The colloid-containing electrolytically reduced water was taken out.

The active oxygen scavenging ability of the produced colloid-containing electrolytically reduced water and ultrapure water as a control was measured by the Fenton reaction method. The Fenton reaction method is a method of measuring .OH in active oxygen, generating .OH using hydrogen peroxide with iron as a catalyst, and exciting .luminum, which is a luminescent reagent, with .OH to generate luminescence generated at that time. This is a method of utilizing the decrease in active oxygen. A specific operation method is as follows: 0.1 M Tris-HCl buffer (pH 7.8), 2.5 μM luminol, 5 μM in a cell of a high-sensitivity chemiluminescence measuring device (CLD-110) manufactured by Tohoku Electronics Sangyo Co., Ltd. DTPA (diethylethylene)
mine-N, N, N ', N ", N'"-pentaac
5 μM FeSO ₄ , 50 μM
After adding hydrogen peroxide of 2 to a final concentration, a test sample was added to adjust the total amount to 2 ml, and the change in luminescence intensity was measured. The measurement result is shown in FIG. As is apparent from the measurement results, the emission intensity of the control ultrapure water is 6.2 × 10 ⁵ , whereas the emission intensity of the colloid-containing electrolytically reduced water of this example is 1.5 × 10 ⁵ . Yes, 75% active oxygen scavenging ability was observed (Fig. 3).

Further, in order to investigate the stability of the active oxygen scavenging ability, the change with time was measured. The measurement result is shown in FIG. 7. As is clear from the results of FIG. 7, the active oxygen scavenging ability was maintained at the same level even after 21 days (FIG. 7), and therefore, the stable active oxygen scavenging ability can be exerted over a long period of time. all right.

Example 2 An electrolyte (NaOH) was dissolved in ultrapure water to obtain a 5 mM NaOH aqueous solution. Using this device (TI-8000 manufactured by Nippon Trim) consisting of one electrolytic bath,
Electrolysis was carried out at 0.13 A / cm ² , and cathode water was taken out from the cathode chamber.

The platinum colloid prepared in Example 1 was used in a concentration of 0.02 ppm, 0.2 ppm and 2 pp.
After adding to the cathode water so that it became m, the active oxygen scavenging ability was measured. The active oxygen scavenging ability was measured by the Fenton reaction method, hydrogen peroxide method and HX-XOD method. In any method, measurement was performed using a high-sensitivity chemiluminescence measuring device CLD-110 manufactured by Tohoku Electronics Industrial Co., Ltd.

In the principle and operation method of the Fenton reaction method
This is as described above. In addition, the hydrogen peroxide method
H out of active oxygen₂O₂Is a method of measuring
FeSO of DTPA from the reaction solution of Enton's reaction_FourMixed liquid
Using the removed reaction solution,₂O ₂From Luminor
It measures light.

The HX-XOD method uses O ₂ · − among active oxygen.
Is a method for measuring O ₂ · − by using HX (hypoxanthine) as a substrate and XOD (xanthine oxidase).
Is generated, O ₂ · − excites CLA (manufactured by Wako Pure Chemical Industries, Ltd.), which is a luminescent reagent, and the decrease in active oxygen is observed by utilizing the luminescence generated at that time. A specific operation method is as follows: 0.2 μM phosphate buffer (pH 7.8), 1 μM CLA, 5 U / m in a cell of CLD-110.
After adding 1 L of XOD and 10 μM of HX to a final concentration, a test sample was added, and the total amount was adjusted to 2 ml, and then the change in luminescence intensity was measured. The measurement result by the Fenton reaction method is shown in FIG. 4 (a), the measurement result by the hydrogen peroxide method is shown in FIG. 4 (b), and the measurement result by the HX-XOD method is shown in FIG. 4 (c).

As is clear from the measurement results, the active oxygen scavenging ability of about 65 to 90% was recognized by adding about 2 ppm of platinum colloid. Further, when autoclave treatment was carried out to examine the stability of the active oxygen scavenging ability, no significant change was observed in the active oxygen scavenging ability between the single treatment and the double treatment by the autoclave (data not shown). Therefore, it was found that the active oxygen scavenging ability is stable.

Example 3 DCFH-DA (dichlorofluorescein diacetate), which reacts with active oxygen and emits fluorescence, as to how the active oxygen concentration in cells is affected by the colloid-containing electrolytically reduced water of the present invention. In vitro
It was evaluated by. As the cells, HT1080 cells (highly metastatic human fibrosarcoma cell line) obtained from Cell Bank (ATCC (USA)) were used.

An apparatus in which an electrolyte (NaOH) was dissolved in ultrapure water to obtain a 5 mM NaOH aqueous solution, and then cathode water shown in FIG. 2 (c) was allowed to pass through three electrolytic cells many times. (Circulation electrolytic reduction device), 0.13 A / cm ²
At 60 ° C. for 60 minutes to obtain cathode water from the cathode chamber. 0.5 p of the platinum colloid produced in Example 1 was added to this cathode water.
pm was added. In the MEM medium containing 10% fetal bovine serum using the obtained colloid-containing electrolytically reduced water,
After culturing HT1080 cells for 1 hour, the fluorescent reagent DCF
H-DA was added, and the fluorescence intensity was measured with a confocal laser biological microscope (Molecular Dynamic).
As a control, ultrapure water alone or ultrapure water containing 0.5 ppm of the platinum colloid produced in Example 1 was used. The measurement results are shown in FIG.

As is clear from the measurement results, the fluorescence intensity of ultrapure water was 255, and platinum colloid was added to ultrapure water in an amount of 0.1%.
The fluorescence intensity was 246, which did not change much even when 5 ppm was added, but the fluorescence intensity was 124 in electrolytic reduced water containing 0.5 ppm of platinum colloid, which was 4 in the case of ultrapure water.
It fell to 9%. Therefore, it was found that the electrolytically reduced water containing the platinum colloid reduced the intracellular active oxygen concentration and changed it to the reduced state.

The embodiments and examples disclosed this time must be considered as illustrative in all points and not restrictive. The scope of the present invention is shown not by the above description but by the claims, and is intended to include meanings equivalent to the claims and all modifications within the scope.

[0045]

EFFECTS OF THE INVENTION According to the present invention, it is possible to provide a stable colloid-containing electrolytically reduced water having a strong antioxidant effect and a method for producing the same.

Since the electrolytically reduced water containing the hydrogen storage metal colloid of the present invention has a strong antioxidative effect, it can be used for the treatment and prevention of cancer and diabetes, and the growth of bacteria can be suppressed. It can also be used for the purpose of protecting living bodies such as cells from oxidative stress.

[Brief description of drawings]

FIG. 1 is a schematic diagram showing an electrolytic cell used for electrolysis of water.

FIG. 2 is a schematic diagram showing an electrolysis cell in which the efficiency of electrolysis of water is enhanced. (A) is a device (TI-7000S manufactured by Nippon Trim) in which two electrolysis cells are connected in series, and (b) is , A device in which three electrolytic cells are connected in series (TI-70 manufactured by Nippon Trim
00 3S), and (c) shows a device (circulation electrolytic reduction device) in which electrolytic reduced water is circulated and passes through three electrolytic cells many times.

FIG. 3 is a diagram showing the ability of electrolytic reduced water containing platinum colloid to eliminate active oxygen.

FIG. 4 is a diagram showing a change in active oxygen scavenging ability with a change in concentration of platinum colloid, (a) shows a measurement result by a Fenton reaction method, (b) shows a measurement result by a hydrogen peroxide method, Moreover, (c) shows the measurement result by the HX-XOD method.

FIG. 5 is a diagram showing the influence of intracellular active oxygen by electrolytically reduced water containing platinum colloid.

FIG. 6 is a diagram showing a surface state of a platinum colloid by a transmission electron microscope.

FIG. 7 is a diagram showing changes over time in the active oxygen scavenging ability.

[Explanation of symbols]

1 1st electrolysis tank, 2 2nd electrolysis tank, 3 3rd electrolysis tank, 10 electrolysis layer, 11 cathode, 12 cathode chamber, 13
Anode, 14 Anode chamber, 15 diaphragm.

─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. ⁷ Identification code FI theme code (reference) A61P 3/10 A61P 35/00 35/00 43/00 111 43/00 111 C25B 9/00 R (72) Inventor Itsudo Otsubo 1-8-34 Oyodochu, Kita-ku, Osaka City F-term in Japan Trim Co., Ltd. (reference) 4C086 AA01 AA02 HA07 HA10 HA12 MA02 MA04 MA17 NA14 ZB26 ZC02 ZC35 4C087 AA01 AA02 BA01 CA01 MA02 MA17 NA14 ZB26 ZC02 ZC35 4K021 AB25 BA02 BA16 BC01 BC09 DB01 DB07 DC15

Claims (7)

[Claims] 1. Colloid-containing electrolytic reduced water, which is electrolytically reduced water obtained by electrolyzing an aqueous electrolyte solution and contains a colloid of a hydrogen storage metal as a carrier of active hydrogen. 2. The electrolytically reduced water containing colloid according to claim 1, wherein the hydrogen storage metal colloid is a platinum colloid, a palladium colloid or a vanadium colloid. 3. A step of adding a hydrogen storage metal colloid to an aqueous solution containing an electrolyte, a step of introducing the aqueous solution into each of a cathode chamber and an anode chamber separated by a diaphragm, and immersing a cathode in the cathode chamber. The step of electrolyzing the aqueous solution by energizing between the cathode and the anode while the anode is immersed in the anode chamber, and the step of extracting the electrolytically reduced water obtained in the cathode chamber, And a method for producing electrolytically reduced water containing colloid. 4. A step of introducing an aqueous solution containing an electrolyte into each of a cathode chamber and an anode chamber separated by a diaphragm; and a step of immersing the cathode in the cathode chamber and immersing the anode in the anode chamber. By energizing between the anode and the anode, a step of electrolyzing the aqueous solution, a step of extracting cathode water obtained in the cathode chamber, a step of adding a hydrogen storage metal colloid to the cathode water, And a method for producing electrolytically reduced water containing colloid. 5. The method of producing electrolytically reduced water containing colloid according to claim 3, wherein the hydrogen storage metal colloid is a platinum colloid obtained by reducing hexachloroplatinic acid (H ₂ PtCl ₆ ). 6. The hydrogen storage metal colloid is a chloride chloride.
Radium (PdCl ₂) Is obtained by reducing
The roller according to claim 3 or 4, which is a palladium colloid.
Id-containing electrolytic reduced water manufacturing method. 7. The method for producing electrolytically reduced water containing colloid according to claim 3, wherein the hydrogen storage metal colloid is a vanadium colloid obtained by reducing vanadium chloride (VCl ₂ ).