JP2003267857A - Skin care preparation and composition of skin care preparation - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a skin care preparation having excellent prophylaxis and amelioration of pigmentation, bleaching and/or skin beautifying effects improving transparency of the skin or cell activating effects on prophylaxis and amelioration of wrinkles and flabbiness and effects of SOD (superoxide dismutase)-like actions. <P>SOLUTION: The skin care preparation comprises an extract from cultured cells of Lithospermum officinale L. var. erythrorhizon Maxim. as a bleaching and/or skin beautifying ingredient, a cell activating ingredient or an ingredient of SOD (superoxide dismutase)-like actions. <P>COPYRIGHT: (C)2003,JPO

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to an external preparation for skin containing an extract of cultured cells of purple purple, more specifically,
By containing an extract of cultured cells of purple, whitening and / or preventing and improving pigmentation and improving the transparency of skin
Further, the present invention relates to a skin external preparation excellent in a skin-beautifying effect, a cell activating effect for preventing and improving wrinkles and tarmi and a SOD (super oxide dismutase) -like effect.

[0002]

2. Description of the Related Art Conventionally, external skin preparations such as emulsions, creams, lotions, packs, detergents, dispersions, ointments, liquid preparations, aerosols, patches and poultices have a predetermined medicinal effect. The medicinal component is added for the purpose. For example, ascorbic acid, placenta extract, glutathione, in order to prevent or ameliorate skin darkening caused by sunburn, stains caused by pigmentation, freckles, etc.
Whitening ingredients such as hydroquinone and skin beautifying ingredients have been added to the skin external preparation.

Further, in order to prevent or improve wrinkles and tarmi of the skin caused by aging, exposure to ultraviolet rays, firmness and deterioration of elasticity, cell activating agents such as vitamin A, soybean extract and seaweed extract are used. , SOD-like agents such as vitamin E, rutin, and ginkgo biloba extract were added.

[0004]

However, with these medicinal components, the desired medicinal effects may not be obtained due to insufficient effects, or alteration in the formulation, etc. Was desired.

[0005]

[Means for Solving the Problems] As a result of intensive investigations by the present inventors on components that can be used as the medicinal components of external preparations for skin such as cosmetics, the extract of cultured purple cells of Murasaki suppresses melanin production. Action, cell activation action, S
It has been found that it has an OD-like action and is excellent as a whitening and / or skin beautifying component and a preventive and ameliorating component such as wrinkles and tarmi.

Furthermore, the present inventors have combined the extract of cultured purple cells with other medicinal ingredients such as a whitening agent, an antioxidant, an anti-inflammatory agent, a cell activator, and an anti-ultraviolet agent to give skin. The present invention has been completed by finding that a superior effect can be obtained as an external preparation composition.

That is, the present invention provides an external preparation for skin containing an extract of cultured purple cells of Murasaki as a whitening and / or skin beautifying component, as a cell activating component or as an SOD-like acting substance.

The present invention further comprises the following components (A) and (B) (A) an extract of cultured cells of Murasaki (B) a whitening agent, an antioxidant, an anti-inflammatory agent, a cell activator, and an anti-UV agent. It is intended to provide a skin external preparation composition containing one or more medicinal components selected from the group consisting of:

[0009]

BEST MODE FOR CARRYING OUT THE INVENTION The skin external preparation and the skin external preparation composition used in the skin external preparation composition of the present invention include leaves, roots and stems from purple perennial belonging to the genus Murata (Boraginaceae). It is obtained by removing a part of the tissue, etc., culturing it by a conventional method, and then extracting the cultured cells or culture solution.

As an example of the raw material purple, there is, for example, lithospermum erythrone.
orhizon) and the like, and the production area thereof is not particularly limited.
The root of this plant is called purple root, and it is known that the extract in China has anti-inflammatory, anti-edema and anti-tumor effects.

The method for culturing tissue from purple squirrel and culturing it is not particularly limited, but a preferable method is as follows.
For example, the following method may be mentioned. That is, first, a part of the tissue of purple, for example, callus was aseptically obtained from the cotyledon, and from this, a strain in which the p-O-β-D-glucosylbenzoic acid (PHBOG) content was maintained high was obtained. select.

Next, this strain is subcultured every 14 days to grow cells, and the subcultured cells are further cultured in a caffeic acid derivative production-inducing medium, for example, a liquid medium of M-9, to give naphthoquinone. Examples thereof include a method of obtaining a cell line that does not exhibit the original purple color and has a high caffeic acid derivative content.

The preparation of the extract from the purple cultivated cells obtained as described above is not particularly limited. For example, it can be obtained by drying the culture cells or the culture solution and then extracting the extract with a suitable extraction solvent. To be

Examples of the extraction solvent used for the extraction of the purple cultivated cells include water, lower monohydric alcohols (methyl alcohol, ethyl alcohol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, etc.), liquid Polyhydric alcohols (glycerin, propylene glycol, 1,3-butylene glycol, etc.), lower alkyl esters (ethyl acetate, etc.), hydrocarbons (benzene, hexane, pentane, etc.), ketones (acetone, methyl ethyl ketone, etc.), ethers ( Diethyl ether, tetrahydrofuran, dipropyl ether, etc.), acetonitrile, etc., and these can be used alone or in combination of two or more.

The extraction method of the purple cultivated cells is not particularly limited, but an example of a preferable extraction method is ethyl alcohol having a water content of 0 to 100 vol% or 1,
A method in which 3-butylene glycol is used at room temperature or after heating and extraction is performed for 1 to 5 days and then filtered, and the obtained filtrate is allowed to stand for another week to be aged and then filtered again can be mentioned. .

The thus-obtained cultured cell extract of purple violet has a slightly different property from the extract obtained directly from the plant purple violet. For example, the extract directly extracted from the purple root exhibits a purple color derived from naphthoquinone,
Cultured cell extract of purple roots is yellow to dark brown.

The extract of the purple cultured cells (component (A)) obtained as described above is used as a whitening and / or skin beautifying component or cell activating component, SOD-like acting component, according to a conventional method and in a conventional manner. A skin external preparation can be obtained by blending it with various forms of bases used for the skin external preparation and formulating it.

The content of the component (A) in the external preparation for skin is preferably 0.00001 to 5% by mass (hereinafter simply referred to as "%") as a dry solid content based on the entire external preparation for skin. It is more preferably 0.0001 to 2%. When the content is within this range, the cultured cell extract can be blended stably, and high drug effect can be exhibited. Further, when the extract is used, the concentration of the extract is not limited as long as the content of the dry solid matter as the solute is within the above range.

Further, the component (A) is further combined with another medicinal component to give a skin external preparation composition.
You can give a better effect

In the present invention, the other medicinal component (component (B)) used in combination with the component (A) is selected from a whitening agent, an antioxidant, an anti-inflammatory agent, a cell activator, and an ultraviolet protective agent. It is a thing. Specific examples of the medicinal agents include those shown below. Here, the "derivative" includes a formable ester or salt.

(Whitening agent) As a whitening agent, vitamin C and its derivative (L-ascorbyl dipalmitate,
L-ascorbic acid alkyl ester such as tetraisopalmitic acid L-ascorbyl, L-ascorbic acid phosphoric acid ester, L-ascorbic acid sulfuric acid ester, etc.), placenta extract, glabridin, glabrene, liquiritin,
Isoliquiritin and licorice extract containing these, Yokuinin (pearl barley) extract, Scutellaria baicalensis (Ougon) extract, seaweed extract (kelp, mackerel, wakame, hijiki, hibamatata, shimeji, torokurombu, kajime, tsurame, chigaiso, hondarawa, Brown algae such as giant kelp; red algae such as agar beetle, giant cucumber, giraffe, tsunomata, horse mackerel, Usbanori, Asakusanori, Matsunori, Tosakamatsu, Funori, gono-ori, kaimensou, igisu, ego-nori , Green algae such as kawaranori, marimo, cynomolgus, scorpionfish, futojuzumo, tamajuzumo, human aegusa, amidoro, etc .; cyanobacteria such as spirulina), sempukuka extract, grape extract, wheat extract, tomato Distillate, carotenoids (carotene, lycopene, etc.), agarose, oligosaccharides, hydroquinone and derivatives thereof, cysteine and derivatives thereof, asparagus extract, bistorta officinalis extract, R. multiflora (rose fruit) extract, Siberian ginseng extract,
Pea extract, chamomile extract, cabbage extract, orange extract, raspberry extract, kiwi extract,
Clara extract, coffee extract, sesame oil, sesame oil, gokahi extract, rice extract, rice bran extract,
Saishin extract, hawthorn extract, sunpens extract (Kawaraketsumei) extract, peony extract, white lily extract, mulberry (Souakuhi) extract, Touki extract, beech extract, beech bud extract, blackcurrant extract,
Hop extract, Maikai squid (Maikai, Hermanus) extract, Mokka (Bokeh) extract, Yukinoshita extract, Tea extract (Oolong tea, black tea, green tea, etc.), Reishi extract, Microbial fermentation metabolite, Soybean extract, Examples include molasses extract and Rakan fruit extract. (In the parentheses, other names of plants, names of crude drugs, etc. are shown.)

Of these whitening agents, particularly preferred are vitamin C and its derivatives, licorice extract, adlay (Yokuinin) extract, wheat extract, mulberry (sophoraceae) extract, seaweed extract, tea extract. Things can be mentioned.

(Antioxidant) As an antioxidant, vitamin E and its derivative (dl-α (β, γ)-
Tocopherol, dl-α-tocopherol acetate, nicotinic acid-dl-α-tocopherol, linoleic acid-dl
-Α-tocopherol, tocopherols such as dl-α-tocopherol succinate and its derivatives, ubiquinones and the like, vitamin A and its derivatives (retinol palmitate, retinol acetate and its retinol and its derivatives,
Dehydroretinal and other retinals and their derivatives), carotenoids (carotene, lycopene, etc.), quercetin, dibutylhydroxytoluene, butylhydroxyanisole, vitamin B and its derivatives (thiamine hydrochloride, thiamine sulfate, riboflavin, riboflavin acetate, pyridoxine hydrochloride) , Pyridoxine dioctanoate, flavin adenine dinucleotide, cyanocobalamin, folic acid, nicotinic acid such as nicotinamide, benzyl nicotinate, choline, etc., vitamin C and its derivatives (L-ascorbyl dipalmitate and tetraisopalmitin Acid L-ascorbic acid alkyl ester such as L-ascorbyl, L-ascorbic acid phosphoric acid ester,
L-ascorbic acid sulfate, etc.), vitamin D and its derivatives (ergocalciferol, cholecalciferol, dihydroxystanal, etc.), rutin, thiotaurine, taurine, hydroquinone and its derivatives, histidine, catechin and its derivatives, glabridin, glabrene. , Liquiritin, isoliquiritin and licorice extract containing them, glutathione and its derivatives, gallic acid and its derivatives, cucumber extract, cayx extract, gentian (gentian) extract, genus ginger extract, cholesterol and its derivatives, hawthorn extract Substance, peony extract, superoxide dismutase, ginkgo extract, Scutellaria baicalensis (Sweet) extract, carrot extract, squid
Hermanus) extract, Sampengs (Kawara Tsutsumei) extract, Tormentilla extract, Parsley extract, Grape extract, Button (Panpi) extract, Mannitol, Mokka (Bokeh) extract, Melissa extract, Yashajitsu (Yasa) extract , Yukinoshita extract, rosemary (mannen wax) extract, lettuce extract, tea extract (oolong tea, black tea, green tea, etc.), microbial fermentation metabolites, seaweed extract, Reishi extract, eggshell membrane extract, placenta extract Thing, an extract of Rakan fruit, and the like. (In the parentheses, other names of plants, names of crude drugs, etc. are shown.)

Among these antioxidants, particularly preferred are vitamin E and its derivatives, vitamin C and its derivatives, rutin, yashajitsu extract, yukinoshita extract, mite squid extract, superoxide dismutase, ginkgo extract. , Glutathione and its derivatives, histidine, mannitol, and carotenoids.

(Anti-inflammatory agent) Anti-inflammatory agents include glycyrrhizic acid and its derivatives, glycyrrhetinic acid and its derivatives, vitamin B and its derivatives (thiamine hydrochloride, thiamine sulfate, riboflavin, riboflavin acetate, pyridoxine hydrochloride, pyridoxine). Dioctanoate, flavin adenine dinucleotide, cyanocobalamin, folic acid, nicotinic acid amide, nicotinic acid such as benzyl nicotinate, choline, etc.), aloe extract, acitaba extract, altea extract, arnica extract, sulfur and Derivatives thereof, nettle extract, Chinese mackerel extract, turmeric extract, yellowfin extract, Hypericum perforatum extract, chamomile extract, comfrey extract, honeysuckle (Kinginka)
Extract, watercress extract, salvia (sage) extract,
Dioscorea japonica extract, perilla extract, birch extract, elderberry extract, cattail (Phyllum esculentum) extract, mukurodi extract, eucalyptus extract, mugwort extract, astragalus extract, chondroitin sulfate and its derivatives, zinc oxide Etc. (In the parentheses, other names of plants, names of crude drugs, etc. are shown.)

Among these anti-inflammatory agents, glycyrrhizic acid and its derivatives, glycyrrhetinic acid and its derivatives, vitamins B and their derivatives are particularly preferable.

(Cell activating agent) Cell activating agents include vitamin A and its derivatives (retinol palmitate, retinol acetate and other retinol and its derivatives, dehydroretinal and other retinal and its derivatives),
Carotenoids (carotene, lycopene, etc.), vitamin B and its derivatives (thiamine hydrochloride, thiamine sulfate, riboflavin, riboflavin acetate, pyridoxine hydrochloride, pyridoxine dioctanoate, flavin adenine dinucleotide, cyanocobalamin, folic acid, nicotinic acid amide, Nicotinic acids such as benzyl nicotinate, cholines, etc.), vitamin C and its derivatives (dipalmitic acid L-
L-ascorbic acid alkyl ester such as ascorbyl or tetraisopalmitic acid L-ascorbyl, L-ascorbic acid phosphoric acid ester, L-ascorbic acid sulfuric acid ester, etc.), ribonucleic acid and its salt, deoxyribonucleic acid and its salt, α- and γ-linolenic acid, xanthine and its derivatives (caffeine, etc.), almond extract, asparagus extract, amino acid and its derivatives (serine, glutamic acid, theanine, hydroxyproline, pyrrolidonecarboxylic acid, etc.), apricot (kyonin) extract ,, Ginkgo biloba extract, docosahexaenoic acid and its derivatives, eicosapentaenoic acid and its derivatives, Yellowfin (Oak)
Extract, barley (bakuga) extract, malt root extract, kiwi extract, cucumber extract, citric acid, lactic acid, malic acid, succinic acid, shiitake extract, horsetail extract, assembly extract, soybean extract, Jujube extract
Centella asiatica extract, capsicum extract, quince extract, tomato extract, garlic extract, carrot extract, hinokitiol, bud extract, grape seed oil, beech extract, beech bud extract, peach extract, eucalyptus Extract, lily extract, lettuce extract, lemon extract, rosemary (mannen wax) extract, animal-derived extract (smolt extract such as squid, shell extract, shell meat extract, fish meat extract, chicken cob extract Products, silk proteins and their degradation products, placenta extracts, serum deproteinization extracts, royal jelly, lactoferrin or its degradation products), yeast extracts, microbial fermentation metabolites (derived from lactic acid bacteria, bifidobacteria, etc.), Reishi extract Etc. (In the parentheses,
Other names of plants, names of crude drugs, etc. are listed. )

Of these cell activating agents, particularly preferred are vitamin A and its derivatives, and vitamin C.
And its derivatives, vitamin B and its derivatives, citric acid, malic acid, pyrrolidonecarboxylic acid, chicken cob extract, serum deproteinization extract, yeast extract, microbial fermentation metabolites (derived from lactic acid bacteria, bifidobacteria, etc.), Reishi extract Things can be mentioned.

(Ultraviolet ray preventive agent) As an ultraviolet ray preventive agent, 2-ethylhexyl paramethoxycinnamate,
4-tert-butyl-4′-methoxydibenzoylmethane, oxybenzone and its derivatives (2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-)
Methoxybenzophenone-5-sulfonic acid, 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid sodium salt, etc.), titanium oxide, fine particle titanium oxide, zinc oxide, fine particle zinc oxide and the like. If the inorganic powder such as titanium oxide or zinc oxide is a fine particle, a higher effect is exhibited.

Among these UV inhibitors, particularly preferable are 2-ethylhexyl paramethoxycinnamate, titanium oxide, fine particle titanium oxide, zinc oxide and fine particle zinc oxide.

In the composition for external preparation for skin of the present invention, the content of the component (A) may be the above-mentioned level, and the content of the medicinal component of the component (B) varies depending on the kind of the medicinal agent. The following ranges are preferred. When the content is within these ranges, it is higher when it is combined with the extract of the cultured cell of Murasaki, which is the component (A), without affecting the preparation and stability of the component (A) in the preparation with time. A whitening and / or beautiful skin effect can be obtained, and an anti-aging effect can be exerted.

That is, the content of the whitening agent in the external preparation for skin of the present invention is preferably 0.00001 to 10%, more preferably 0.0001 to 5 with respect to the entire external preparation composition for skin. % Range. When a plant extract or the like as a whitening agent is used as it is, the dry solid content may be within this range. Within this range, a skin external preparation composition can be obtained in which more excellent whitening and / or skin beautifying effects are exhibited and the usability is good.

The content of the antioxidant in the skin external preparation composition of the present invention is based on the total skin external preparation composition.
Preferably 0.00001 to 5%, more preferably 0.
It is in the range of 0001 to 3%. When a plant extract or the like as an antioxidant is used as an extract as it is, the dry solid content may be within this range. Within this range, an excellent anti-oxidant effect is exhibited, and a skin external preparation composition having an excellent whitening / skinning effect and a preventive / improving effect against wrinkles, tarmi and the like can be obtained.

Further, the content of the anti-inflammatory agent in the skin external preparation composition of the present invention is preferably 0.00001 to 5%, more preferably 0.0001 to 3%, based on the total skin external preparation composition. % Range. When a plant extract or the like as an anti-inflammatory agent is used as an extract as it is, the dry solid content may be within this range. Within this range, a skin external preparation composition having an excellent anti-inflammatory effect and an excellent whitening / skinning effect and an effect of preventing and improving wrinkles, tarmi and the like can be obtained.

Furthermore, the content of the cell activating agent in the external preparation for skin of the present invention is preferably 0.00001 to 5%, more preferably 0.0001 to 5% with respect to the entire external preparation composition for skin. It is in the range of 3%. When a plant extract or the like as a cell activator is used as it is as an extract, the dry solid content may be within this range. Within this range, a skin external preparation composition exhibiting an excellent effect of improving rough skin and exhibiting a more effective whitening / skinning effect and an effect of preventing and improving wrinkles, tarmi and the like can be obtained.

Furthermore, the content of the ultraviolet protective agent in the skin external preparation composition of the present invention is preferably 0.001 to 30%, more preferably 0.01 to 30%, based on the total skin external preparation composition. It is in the range of 25%. Within this range, a skin external preparation composition that exhibits an excellent anti-ultraviolet effect and exhibits a more effective whitening / skinning effect and a prophylactic / improving effect against wrinkles, tarmi and the like can be obtained.

In the present invention, these whitening agents, antioxidants, anti-inflammatory agents, cell activators, and UV inhibitors can be used alone or in combination of two or more.

The skin external preparation and the skin external preparation composition of the present invention are prepared by using the essential component (A) or the component (A) and the component (B) in a usual skin external preparation according to a conventional method. It can be prepared by combining the known optional components described above with various forms.

The optional components that can be used include
Water, alcohol, oils, surfactants, thickeners, powders, chelating agents, pH adjusters, various medicinal agents, extracts derived from plants and animals, microorganisms, cosmetics such as fragrances, quasi-drugs, and external medicines. Various components usually used in the above can be appropriately added within a range that does not impair the effects of the present invention. Of these, specific examples are shown below.

The alcohol can be added for the purpose of dissolving, cooling, antiseptic, moisturizing, etc. as long as it does not overlap with the essential components. Monohydric alcohols such as ethyl alcohol, glycerin and 1,3-butylene glycol can be added. And other polyhydric alcohols can be used.

The oil agent can be used regardless of its origin and properties as it improves usability and usability.
For example, liquid paraffin, squalane, triglyceride oil, ester oil, waxes, fatty acids, higher alcohols, silicone oil, fluorinated oil, various waxes and the like.

The surfactant is used for emulsification and solubilization of oils and the like, and anionic, cationic, nonionic and amphoteric surfactants can be used.

As the thickener, it is possible to use carboxyvinyl polymer, carrageenan, agar, xanthan gum, dextrin fatty acid ester, organically modified clay mineral, etc. regardless of whether they are derived from a chemically synthesized product or a natural product. or,
These components can be used not only for adjusting the viscosity of the system, but also for gelling, moisturizing, film formation and the like.

The powder is added regardless of the shape, the size of the particles, the presence or absence of porosity, the crystal structure, etc. in order to improve usability and usability. This powder may be compounded or surface treated, such as talc, mica, sericite,
Inorganic powders such as silicic acid anhydride, organic powders such as nylon powder, fish scale foil, pearl pigments such as bismuth oxychloride, inorganic pigments such as iron oxide, carbon black, ultramarine, tar pigments and their lakes, natural pigments, etc. It is used according to the application.

In order to prevent the quality deterioration of the components in the system, EDT
A chelating agent such as A and a pH adjusting agent with a buffer such as lactic acid-sodium lactate can also be used.

Examples of the medicinal agents include synthetic products, those derived from animals and plants, and microorganisms. For example, as antibacterial agents and bactericides, for the purpose of preventing and improving acne, benzoic acid, sodium benzoate, paraoxybenzoic acid ester, parachlorometacresol, benzalkonium chloride, phenoxyethanol, isopropylmethylphenol, etc. are used. To be By blending these, pigmentation due to bacterial skin inflammation such as acne is suppressed,
Further high whitening and / or beautiful skin effect can be exhibited.

Examples of moisturizing agents include proteins or their derivatives or hydrolysates and their salts (collagen, elastin, keratin, etc.), mucopolysaccharides and their derivatives (hyaluronic acid, etc.), saccharides (sorbitol, erythritol, trehalose, inositol). ,glucose,
Xylitol, sucrose and its derivatives, dextrin and its derivatives, honey, etc.), D-panthenol and its derivatives, glycolipids, ceramides, licorice extract, avocado extract, hot spring water, Astragalus membranaceus extract, Astragalus membranaceus extract Substance, Ononis extract, oats extract, quince seed extract, gardenia extract, kumazasa extract, grapefruit extract, burdock extract,
Cactus extract, Sophoraceae extract, Zygo extract, Spirea extract, Ginger extract, Ginger extract, Peppermint (peppermint) extract, Zenia mallow (Pleurotus cornucopiae) extract, Prunus chinensis extract, Prickly pear (thyme) Extract, camellia extract, Astragalus extract, Dokudami extract, peppermint extract, hamamelis extract, rose extract, cypress extract, sunflower extract, coltsfoot extract, butchers bloom extract, prune extract, loofah extract Substance, Bodaiju extract, pine extract, quince extract, horse chestnut extract, mucin, cornflower extract, lime extract, lavender extract, apple extract, soybean and egg-derived phospholipids, urea, Rakan fruit extract, Examples include seaweed extracts.

Furthermore, as a moisturizing (emollient) agent by sealing the skin surface, jojoba oil, macadamia nut oil, olive oil, apricot kernel oil, persic oil, safflower oil, sunflower oil, avocado oil, medhome oil, camellia oil, almond oil. , Sesame oil, sesame oil, borage (borage) oil, cocoa butter, shea butter and the like. By blending these moisturizers, higher whitening and / or skin beautifying effects can be exhibited, and transparent skin can be realized. (In the parentheses, other names of plants, names of crude drugs, etc. are shown.)

As the blood circulation promoter, capsicum tincture, γ-oryzanol and the like are used for the purpose of promoting melanin excretion by promoting blood flow in the skin, and lipase, papain and the like are used as enzymes. By blending these, higher whitening and / or skin beautifying effect can be exhibited.

The compounding form of the skin external preparation and the skin external preparation composition of the present invention thus obtained is not particularly limited, and examples thereof include emulsions, creams, lotions, beauty essences, packs, detergents, makeup cosmetics, and dispersions. It may be a cosmetic in the form of a liquid, an ointment, a liquid agent, an aerosol, a patch, or the like, or an external drug or the like.

[0051]

EXAMPLES The present invention will be described in more detail with reference to Reference Examples, Test Examples and Examples, but the present invention is not limited thereto.

Reference Example 1 Lithospermum eryth
(orrhizon) -derived cultured cell extract production: Rinsmeier-Skoog (LS) agar solid medium is sterilized in advance with an antiformin solution having an effective chlorine concentration of 2% or a 70 vol% ethanol solution. The 5 to 10 mm square tissue piece of the cotyledon of Murasaki was placed on the plate and statically cultured at 25 ° C. in the dark to obtain a callus of Murasaki. A large number of cultured cells were obtained by repeating this subculture operation. For this cultured cell, p-O-β
The content of -D-glucosylbenzoic acid (PHBOG) was measured, and the M-18TOM strain was obtained as a cell having PHBOG of 2 mg / g (fresh weight) or more.

Next, the M-18T obtained by the above operation
1 g of callus of OM strain (fresh weight) was added to a liquid medium of LS (1 μmol / L indoleacetic acid and 10 μmol / L kinetin as plant hormone, 30 g / L as carbon source).
The mixture was transferred to an Erlenmeyer flask containing 20 mL of sucrose (containing sucrose), and the flask was cultivated on a rotary shaker under the conditions of an amplitude of 25 mm and 100 rpm and subcultured every 14 days to accelerate the growth rate of callus.

In the subculture process of this liquid culture, when the cells were cultured in a liquid medium of M-9 on a trial basis, there were flasks containing cultured cells that produced shikonin and flasks containing cultured cells that did not produce shikonin. The produced cultured cells were referred to as M-18TOM strain, and the non-produced cultured cells were referred to as WM18 strain. Furthermore, when the M-18TOM strain was subcultured, there were cultured cell lines that did not produce shikonin.
This was separated and the TomK2 strain (PHBOG content 2.2 m
g / g (fresh weight)). The TomK2 strain cultured cells were transferred to a culture tank containing 150 L of M-9 liquid medium, and cultured with aeration and stirring at 25 ° C. for 21 days. After culturing,
The cultured cells and the culture solution were separated by filtration, and the obtained cultured cells were air-dried at 60 ° C.

Lithospermum thus obtained
Lithospermum erythrorhizon cultured cells (TomK2 strain) dried product 100 g, purified water, 50
Each 1 L of a vol% ethyl alcohol solution and ethyl alcohol was added, extraction was performed at room temperature for 3 days, and then filtration was performed to obtain an extract of purple cultivated cells. The dry solids of these extracts are shown in Table 1.

[0056]

[Table 1]

Reference Example 2 Preparation of Yokuinin extract: Yokuinin (JP) 10 g
To this, 100 mL of 70 vol% hydrous ethyl alcohol was added, and extraction was performed at room temperature for 3 days. Then, the extract was filtered to obtain Yokuinin extract. At this time, the dry solid content of Yokuinin extract was 0.8%.

Test Example 1 Suppression of melanin production by cell culture and cell viability test:
Mouse-derived B16 melanoma cultured cells were used. Two
An appropriate amount of 10% FBS-containing MEM medium was placed on a single 6-well plate, B16 melanoma cells were seeded, and left standing at 37 ° C. in a carbon dioxide concentration of 5 vol%. On the next day, the final concentration of each extract of the cultured cells of the purple purple obtained in Reference Example 1 was reduced to 0.
(Control), 15, 50, 150, and 1000 μg / mL of the sample preparation solution were added and mixed.

On the 5th day of culture, the medium was exchanged and the sample preparation solution was added again. The next day, the medium was removed, and one dish was washed with phosphate buffer and then collected, and B1
The whiteness of 6 melanoma cultured cells was evaluated according to the following criteria. In addition, as a comparison, the same test was also conducted and evaluated for the Yokuinin extract (obtained in Reference Example 2), which is already known to have a melanin production inhibitory action.

[0060]   (Judgment standard)     Judgment         +: Clearly white with respect to the control.         ±: slightly whiter than the control.         -: The same black color as the control.

Furthermore, regarding the remaining one plate,
After fixing the cells with formalin, 1% crystal violet solution was added and stained. The cell viability for each sample concentration was measured with a monoserator (Olympus). The above results are shown in Table 2.

(Result)

[Table 2]

As is clear from the results of Table 2, the extract of the purple cultured cells obtained in Reference Example 1 of the present invention has a high ability to suppress melanin production and has low toxicity to the cultured cells of B16 melanoma. Was confirmed. Therefore, by applying this to the skin, the cultured cell extract of Murasaki exerts an extremely excellent melanin production inhibitory effect, effectively suppresses skin blackening due to sunburn, stains, freckles, etc., and whitens and It can be expected to obtain a beautiful skin effect.

Reference Example 3 Production of Soybean Extract: 70 vol% to 10 g of soybean seeds
Add 100 mL of water-containing ethyl alcohol and stir at room temperature for 3
After extraction for a day, it was filtered to obtain a soybean extract. At this time, the dry solid content of the soybean extract was 0.5%.

Test Example 2 Cell activation test by cell culture: Human newborn-derived fibroblasts NB1RGB were used. An appropriate amount of medium was collected in a 24-well plate, seeded with fibroblasts NB1RGB, 37
It was allowed to stand at 0 ° C. in a carbon dioxide concentration of 5 vol%. next day,
Each of the extracts obtained in Reference Example 1 was mixed with a sample preparation solution so that the final concentrations were 0 (control), 1, 10, and 100 μg / mL, respectively. The medium of this mixture was exchanged on the 4th day of culture, and the sample preparation solution was added again. On the next day, the medium was removed, the cells were washed with a phosphate buffer and then collected, and the number of fibroblasts NB1RGB grown in each sample preparation was evaluated as a cell growth rate compared with a control. The number of cells is
Counting was performed using a hemocytometer.

For comparison, the same test was carried out on a soybean extract (obtained in Reference Example 3) which has been known to have a cell activating effect. The results are shown in Table 3.

(Result)

[Table 3]

As is clear from the results shown in Table 3, Reference Example 1
It was confirmed that the extract of the cultured cells of Murasaki obtained in 1) had a high cell activation ability to human newborn-derived fibroblasts NB1RGB. Therefore, by applying the extract as a cell activating component to the skin, it is expected to exert an extremely excellent anti-aging effect and effectively improve wrinkles, slackening, etc. of the skin caused by aging, UV exposure, etc. it can.

Reference Example 4 Production of Scutellariae Radix Extract: To 10 g of the whole plant of Scutellaria barbata, 100 mL of ethyl alcohol having a water content of 70 vol% was added,
Extraction was carried out at room temperature for 3 days and then filtered to obtain a sardine extract. At this time, the dry solid content of the Scutellaria extract is 0.
It was 5%.

Test Example 3 Superoxide scavenging effect measurement test: Each extract of the purple cultivated cell obtained in Reference Example 1 and augon extract which has been known to have a superoxide scavenging effect according to the following measuring method. Thing (obtained in Reference Example 4;
A comparative product) was examined for the superoxide scavenging effect. In addition, superoxide dismutase (SO
D) is an in vivo antioxidant enzyme having an action of eliminating superoxide, and the SOD-like action means an action of eliminating superoxide contained in a substance other than SOD. Therefore, the SOD-like action of various samples is measured by, for example, the scavenging rate of superoxide obtained by the following formula (I) and the like, and it can be judged that the higher the value, the better the SOD-like action.

(Measurement method) 0.05 mol / L Sodium carbonate buffer solution (pH 10.2) 2.4 mL was added to the substrate solution [3.0 mmol / L xanthine (0.05 mo)
1 / L dissolved in sodium carbonate buffer)] 0.1 mL,
3.0 mmol / L EDTA 0.1 mL, 0.15%
(W / v) bovine serum albumin 0.1 mL, 0.75
mmol / L Nitroblue tetrazolium 0.1mL
And 0.1 mL of each test sample of 0.3 mg / mL were mixed and left at 25 ° C. for 10 minutes. Then, the enzyme solution [xanthine oxidase solution (about 0.04un with purified water
(diluted to its / mL)] to start the reaction by adding 0.1 mL, and after incubating at 25 ° C. for 20 minutes, 6 mmo
The reaction was stopped by adding 0.1 mL of 1 / L cupric chloride.
Then, the absorbance (A) at 560 nm was measured.

For the control, the absorbance (B) of the sample to which purified water was added instead of the test sample, and for the blank of each sample,
After the reaction was stopped by adding 0.1 mL of 6 mmol / L cupric chloride, the absorbance (C) of the sample to which 0.1 mL of xanthine oxidase was added was measured, and from the following formula (I),
The superoxide scavenging rate was calculated. The results are shown in Table 4.
Shown in.

[0073]

[Equation 1]

(Result)

[Table 4]

As is clear from the results shown in Table 4, Reference Example 1
It was confirmed that each extract of the cultured cells of Murasaki obtained in step 1 showed a high superoxide scavenging activity and was extremely effective in scavenging active oxygen.

Example 1 Cream: A cream was produced using the formulation shown in Table 5 and the following production method. About the obtained cream, the whitening and skin-improving effects obtained when the 50% vol. Ethyl alcohol extract of purple cell culture and a whitening agent, an antioxidant, an anti-inflammatory agent, a cell activating agent, and an anti-UV agent are used in combination by the test method described below. It was confirmed. The results are shown in Table 6.

(Method)

[Table 5]

(Production Method) A. Components (1)-(6) and (11) are mixed and heated to 70 ° C. B. Ingredient (13) is heated and kept at 70 ° C. C. "B." is added to "A.", and components (7) to (1
After mixing 0) and (12), the mixture was cooled to obtain a cream.

(Test Method) For each test cream product, 15 women aged 27 to 54 were used as a panel, and an appropriate amount of the test cream was applied to the face twice daily in the morning and at night for 12 weeks. The whitening and skin beautifying effects of the application were evaluated according to the following criteria.

[0080]   ( Evaluation criteria )       Rating: Content         Effective: The dullness of the skin is less noticeable.       Slightly effective: The skin's dullness has become less noticeable.         Invalid: No change from before use.

(Result)

[Table 6]

As shown in the results of Table 6, the cream of the product of the present invention containing the 50% ethyl alcohol extract of cultured cells of Murasaki, when applied to the skin, causes the generation of "skin" on the skin. It is clear that it can prevent, improve, and make beautiful skin, but further, 50% ethyl alcohol extract of purple cell culture and whitening agents, antioxidants, anti-inflammatory agents, cell activators, UV protection By applying to the skin the products 2 to 7 of the present invention formulated in combination with the agent, it was more excellent than the case of applying the external preparation containing the 50% ethyl alcohol extract of the purple cultivated cells alone. It was confirmed that the appearance of "skin" on the skin was synergistically exhibited and the improvement effect was exhibited, resulting in beautiful skin.

Example 2 Cream: A cream was produced according to the formulation shown in Table 7 and the following production method. About the obtained cream, by the test method described below, the wrinkle-improving effect was confirmed when the purified water extract of purple cell culture and a whitening agent, an antioxidant, an anti-inflammatory agent, a cell activating agent, and an anti-UV agent were used in combination. . The results are shown in Table 8.

(Method)

[Table 7]

(Production Method) A. Components (1) to (6), (8) to (10) and (1
Mix 2) to 13 and heat to maintain 70 ° C. B. Part of component (15) is heated and kept at 70 ° C. C. After adding "B." to "A." and mixing the component (7), the mixture was cooled and the components (11) and (14) were added to obtain a cream.

(Test Method) For each test cream product, 15 women aged 27 to 54 were used as a panel, and an appropriate amount of the test cream was applied to the face twice daily in the morning and at night for 12 weeks. The effect of improving wrinkles by coating was evaluated according to the following criteria.

[0087]   ( Evaluation criteria )       Rating: Content         Effective: Wrinkles on the skin are less noticeable.       Slightly effective: Wrinkles on the skin became less noticeable.         Invalid: No change from before use.

(Result)

[Table 8]

As shown in the results of Table 8, the cream of the present invention containing the extract of the cultured cells of Murasaki can prevent and improve wrinkles by applying these creams to the skin, which is beautiful. It is obvious that the skin is used, and further, the present invention product in which the purple cell culture extract and a moisturizing agent, an antioxidant, an anti-inflammatory agent, a cell activator, and a UV inhibitor are used in combination is applied to the skin. Thus, it was confirmed that, compared with the case of applying the external preparation containing only the purple cell culture extract alone, synergistically excellent effects of preventing and improving wrinkles were obtained, and beautiful skin was obtained.

Example 3 Lotion: A lotion was manufactured by the following formulation and manufacturing method.

(Method) Component mass% 1. Glycerin 10.0 2. 1,3-butylene glycol 6.0 3. Murasaki culture cell ethyl alcohol extract ^{* 3} 1.0 4. Citric acid 0.1 5. Sodium citrate 0.3 6. Purified water Remaining amount 7. Polyoxyethylene hydrogenated castor oil (60 EO) 0.5 8. Ethyl alcohol 8.0 9. Preservative proper amount 10. Fragrance suitable amount * 3: produced in Reference Example 1

(Production Method) A. Components (1) to (6) are mixed and dissolved. B. Components (7) to (10) are mixed and dissolved. C. "A." and "B." were mixed and made uniform to obtain a lotion.

Example 4 Lotion: A lotion was manufactured by the following formulation and manufacturing method.

(Method) Component mass% 1. Glycerin 10.0 2. 1,3-butylene glycol 6.0 3. 0.5 50 vol% ethyl alcohol extract of purple cell culture ^{* 2} 4. Seaweed extract 0.1 5. Citric acid 0.1 6. Sodium citrate 0.3 7. Purified water Remaining amount 8. Polyoxyethylene hydrogenated castor oil (60 EO) 0.5 9. Ethyl alcohol 8.0 10. Preservative proper amount 11. Perfume 0.1 * 2: produced in Reference Example 1

(Production Method) A. Components (1) to (7) are mixed and dissolved. B. Components (8) to (11) are mixed and dissolved. C. "A." and "B." were mixed and made uniform to obtain a lotion.

Example 5 Emulsion: An emulsion was produced by the following formulation and production method.

(Method) Component mass% 1. Sorbitan monostearate 0.3 2. Polyoxyethylene sorbitan monooleate 0.1 (20.EO) 3. Lipophilic type glyceryl monostearate 0.2 4. Stearic acid 0.5 5. Cetanol 0.5 6. Squalane 3.0 7. Liquid paraffin 4.0 8. Glyceryl tri-2-ethylhexanoate 2.0 9. Methyl polysiloxane 1.0 10. Hydrogenated soybean phospholipid 0.1 11. Dl-α-tocopherol nicotinate ^{* 17} 0.05 12. Preservative proper amount 13. Carboxyvinyl polymer aqueous solution (10%) 1.0 14. Sodium hydroxide 0.05 15. Glycerin 5.0 16. 1,3-butylene glycol 7.0 17. Purified water balance 18. Ethyl alcohol 5.0 19. Murasaki cell culture extract 0.1 50 vol% ethyl alcohol extract ^{* 2} 20. L-oxyproline ^{* 18} 0.2 21. Hemp cellulose powder 3.0 22. Perfume suitable amount * 2: manufactured in Reference Example 1 * 17: manufactured by Eisai Co., Ltd. * 18: manufactured by Kyowa Fermentation Co., Ltd.

[0098]   (Production method)     A. The components (13) to (17) are mixed by heating and kept at 70 ° C.     B. The components (1) to (12) are mixed by heating and kept at 70 ° C.     C. Add "B." to "A." and mix to uniformly emulsify.     D. After cooling "C.", components (18) to (22) were added and mixed uniformly.         An emulsion was obtained.

Example 6 Emulsion: An emulsion was produced by the following formulation and production method.

(Method) Component mass% 1. Sorbitan monostearate 0.3 2. Polyoxyethylene sorbitan monooleate 0.1 (20.EO) 3. Lipophilic type glyceryl monostearate 0.2 4. Stearic acid 0.5 5. Cetanol 0.5 6. Squalane 3.0 7. Liquid paraffin 4.0 8. Glyceryl tri-2-ethylhexanoate 2.0 9. Methyl polysiloxane 1.0 10. Hydrogenated soybean phospholipid 0.1 11. Rutin ^{* 19} 0.2 12. Preservative proper amount 13. Carboxyvinyl polymer aqueous solution (10%) 1.0 14. Sodium hydroxide 0.05 15. Glycerin 5.0 16. 1,3-butylene glycol 7.0 17. Purified water balance 18. Ethyl alcohol 5.0 19. Ethyl alcohol extract of purple cell culture ^{* 3} 5.0 20. Carrot extract ^{* 20} 1.0 21. Silicic anhydride 3.0 22. Fragrance suitable amount * 3: manufactured in Reference Example 1 * 19: manufactured by Sigma * 20: manufactured by Ichimaru Falcos

[0101]   (Production method)     A. The components (13) to (17) are mixed by heating and kept at 70 ° C.     B. The components (1) to (12) are mixed by heating and kept at 70 ° C.     C. Add "B." to "A." and mix to uniformly emulsify.     D. After cooling "C.", components (18) to (22) were added and mixed uniformly.         An emulsion was obtained.

Each of the lotions or emulsions obtained in Examples 3 to 6 has excellent stability over time, and when applied to the skin, "sunburn" or spots, freckles and wrinkles due to aging on the skin. It was a lotion and milky lotion that prevented tarmi and gave a beautiful transparent skin.

Example 7 Ointment: An ointment was produced by the following formulation and production method.

(Method) Component mass% 1. Stearic acid 18.0 2. Cetanol 4.0 3. dl-α-tocopherol ^{* 21} 0.2 4. Preservative proper amount 5. Triethanolamine 2.0 6. Glycerin 5.0 7. Purified water Remaining amount 8. Purple cultivated cell purified water extract ^{* 2} 1.0 9. Dipotassium glycyrrhizinate ^{* 10} 0.5 10. Yeast extract 0.5 * 2: manufactured in Reference Example 1 * 10: manufactured by Maruzen Pharmaceutical Co., Ltd. * 21: manufactured by Eisai

[0105]   (Production method)     A. A part of the components (5), (6) and (7) is mixed by heating and kept at 75 ° C.     B. The components (1) to (4) are mixed by heating and kept at 75 ° C.     C. Gradually add "A." to "B."     D. While cooling "C.", components (8) to (dissolved in the balance of component (7)         10) was added to obtain an ointment.

Example 8 Ointment: An ointment was produced by the following formulation and production method.

(Method) Component mass% 1. Stearic acid 18.0 2. Cetanol 4.0 3. Preservative proper amount 4. Triethanolamine 2.0 5. Glycerin 5.0 6. Purified water Remaining amount 7. Murasaki cell culture ethyl alcohol extract ^{* 3} 1.0 8. Touki extract ^{* 23} 0.3 9. Sodium pyrrolidonecarboxylate ^{* 24} 0.3 * 3: manufactured in Reference Example 1 * 23: manufactured by Nippon Powder Chemicals Co., Ltd. * 24: manufactured by Ajinomoto Co., Inc.

[0108]   (Production method)       A. Part of components (4), (5) and (6) are mixed by heating and kept at 75 ° C.              .       B. The components (1) to (3) are mixed by heating and kept at 75 ° C.       C. Gradually add "A." to "B."       D. Component (7) dissolved in the balance of component (6) while cooling "C."           (9) was added to obtain an ointment.

Each of the ointments obtained in Examples 7 and 8 has excellent stability over time, and when applied to the skin, prevents "sunburn" and spots, freckles, and wrinkles and tarmis on the skin due to sunburn. However, it was an ointment that gave a transparent and beautiful skin.

Example 9 Pack: A pack was manufactured according to the following formulation and manufacturing method.

(Method) Component mass% 1. Polyvinyl alcohol 15.0 2. Silicic anhydride 0.5 3. Polyethylene glycol 0.5 4. Polyoxypropylene methyl glucoside 5.0 5. Glycerin 5.0 6. Purified water Remaining amount 7. Ethyl alcohol 20.0 8. Preservative proper amount 9. Purple cultivated cell purified water extract ^{* 1} 0.1 10. Murasaki culture cell ethyl alcohol extract ^{* 3} 0.2 11. Perfume suitable amount * 1: manufactured in Reference Example 1 * 3: manufactured in Reference Example 1

[0112]   (Production method)     A. Components (1) to (6) are mixed, heated to 70 ° C., and stirred.     B. Mix components (7) and (8).     C. After adding "B." to "A.", mixing and cooling, the component (9) was added.         (11) were uniformly dispersed to obtain a pack.

Actual Example 10 Pack: A pack was manufactured according to the following formulation and manufacturing method.

(Method) Component Mass% 1. Polyvinyl alcohol 15.0 2. Silicic anhydride 0.5 3. Polyethylene glycol 0.5 4. Polyoxypropylene methyl glucoside 5.0 5. Glycerin 5.0 6. Purified water Remaining amount 7. Orange flower water ^{* 25} 10.0 8. Ethyl alcohol 20.0 9. Preservative proper amount 10. 0.2 50 vol% ethyl alcohol extract of purple cell culture extract ^{* 2} 11. Licorice extract ^{* 7} 0.5 12. Wheat extract ^{* 26} 0.5 13. Perfume suitable amount * 2: manufactured in Reference Example 1 * 7: manufactured by Maruzen Pharmaceutical Co., Ltd. * 25: manufactured by Esperis Co., Ltd. * 26: manufactured by Seowa Kasei Co., Ltd.

[0115]   (Production method)     A. Components (1) to (7) are mixed, heated to 70 ° C., and stirred.     B. Mix components (8) and (9).     C. After adding "B." to "A.", mixing and cooling, the components (10) to (         13) was uniformly dispersed to obtain a pack.

Each of the packs obtained in Examples 9 and 10 has excellent stability over time, and when applied to the skin, prevents "sunburn" and spots on the skin due to sunburn, freckles, and wrinkles and tarmi due to aging. However, it was a pack that makes the skin beautiful and transparent.

Example 11 Liquid foundation: A liquid foundation was produced by the following formulation and production method.

(Method) Component mass% 1. Liquid lanolin 2.0 2. Liquid paraffin 5.0 3. Stearic acid 2.0 4. Cetanol 1.0 5. Self-emulsifying glyceryl monostearate 1.0 6. 2-Ethylhexyl paramethoxycinnamate 8.0 7. Preservative proper amount 8. Glycerin 5.0 9. Triethanolamine 1.0 10. Carboxymethyl cellulose 0.2 11. Bentonite 0.5 12. Purified water remaining 13. Titanium oxide 6.0 14. Fine particle titanium oxide 2.0 15. Fine particle zinc oxide 5.0 16. Mica 2.0 17. Talc 4.0 18. Color pigment 4.0 19. 0.01 50 vol% ethyl alcohol extract of purple cell culture extract ^{* 2} 20. Mannitol ^{* 27} 0.5 21. Nicotinic acid amide ^{* 28} 0.5 22. Fragrance suitable amount * 2: manufactured in Reference Example 1 * 27: Sigma company * 28: Sigma company

[0119]   (Production method)     A. Components (1) to (7) are mixed and dissolved.     B. Ingredients (13) to (18) were added to "A.", mixed evenly, and heated to 70 ° C.         keep.     C. Components (8) to (12) are uniformly dissolved and kept at 70 ° C.     D. "B." is added to "C." to uniformly emulsify.     E. After cooling "D.", the components (19) to (22) were added, and the liquid solution was added.         I got a foundation.

Example 12 Liquid Foundation: A liquid foundation was produced by the following formulation and production method.

(Method) Component mass% 1. Liquid lanolin 2.0 2. Liquid paraffin 5.0 3. Stearic acid 2.0 4. Cetanol 1.0 5. Self-emulsifying glyceryl monostearate 1.0 6. 2-Ethylhexyl paramethoxycinnamate 8.0 7. 4-tert-butyl-4'2.0-methoxydibenzoylmethane 8. Retinol palmitate ^{* 16} 0.2 9. Preservative proper amount 10. Glycerin 5.0 11. Triethanolamine 1.0 12. Carboxymethyl cellulose 0.2 13. Bentonite 0.5 14. Purified water remaining 15. Titanium oxide 6.0 16. Particulate titanium oxide 2.0 17. Fine particle zinc oxide 5.0 18. Mica 2.0 19. Talc 4.0 20. Yellow iron oxide 1.0 21. Black iron oxide 0.05 22. Bengal 0.5 23. Murasaki cell extract 0.2 ethyl alcohol extract ^{* 3} 24. Acetic acid-dl-α-tocopherol ^{* 14} 0.5 25. Phosphoric acid-L-ascorbyl magnesium ^{* 8} 0.5 26. Fragrance suitable amount * 3: manufactured in Reference Example 1 * 8: Nikko Chemicals * 14: Eisai * 16: Nippon Roche

[0122]   (Production method)     A. Components (1) to (9) and (24) are mixed and dissolved.     B. Ingredients (15) to (22) were added to "A.", mixed evenly, and heated to 70 ° C.         keep.     C. Components (10) to (14) and (25) are uniformly dissolved and kept at 70 ° C.         .     D. "B." is added to "C." to uniformly emulsify.     E. After cooling "D.", components (23) and (26) were added, and a liquid was added.         I got a foundation.

Example 13 Sunscreen Emulsion: A sunscreen emulsion was prepared by the following formulation and production method.

(Method) Component mass% 1. Polyoxyalkylene-modified organopolysiloxane 1.0 2. Dimethyl polysiloxane 5.0 3. Octamethylcyclotetrasiloxane 20.0 4. Isotridecyl isononanoate 5.0 5. 2-Ethylhexyl paramethoxycinnamate 5.0 6. Preservative proper amount 7. Perfume proper amount 8. Fine particle titanium oxide 10.0 9. Particle zinc oxide 10.0 10. Zirconium oxide 5.0 11. Polystyrene powder 3.0 12. Trimethylsiloxysilicic acid 0.5 13. Dipropylene glycol 3.0 14. Ethyl alcohol 10.0 15. Purified water remaining 16. Salt 0.2 17. Purified water extract of purple cell culture cell extract ^{* 1} 2.0 18. Seaweed extract ^{* 29} 3.0 * 1: Manufactured in Reference Example 1 * 29: Made by Philip Rockley

[0125]     (Production method)     A. Components (1) to (12) are mixed and dispersed.     B. Components (13) to (16) are mixed and dispersed.     C. "B." is added to "A." to uniformly emulsify.     D. Add the components (17) and (18) to "C." to give a sunscreen emulsion.         Obtained.

Example 14 Sunscreen Emulsion: A sunscreen emulsion was prepared by the following formulation and production method.

(Method) Component mass% 1. Polyoxyalkylene-modified organopolysiloxane 1.0 2. Dimethyl polysiloxane 5.0 3. Octamethylcyclotetrasiloxane 20.0 4. Isotridecyl isononanoate 5.0 5. 2-Ethylhexyl paramethoxycinnamate 10.0 6. Preservative proper amount 7. Perfume proper amount 8. Fine particle titanium oxide 8.0 9. Fine particle zinc oxide 7.0 10. Zirconium oxide 5.0 11. Polystyrene powder 3.0 12. Trimethylsiloxysilicic acid 0.5 13. Dipropylene glycol 3.0 14. Ethyl alcohol 10.0 15. Purified water remaining 16. Salt 0.2 17. 50 vol% ethyl alcohol extract of purple cell culture extract ^{* 2} 1.0 18. Phosphoric acid-L-ascorbyl magnesium ^{* 8} 3.0 19. Green tea extract ^{* 30} 0.5 * 2: manufactured in Reference Example 1 * 8: Nikko Chemicals * 30: Maruzen Pharmaceuticals

[0128]     (Production method)     A. Components (1) to (12) are mixed and dispersed.     B. Components (13) to (16) are mixed and dispersed.     C. "B." is added to "A." to uniformly emulsify.     D. Components (17) to (19) are added to "C." to obtain a sunscreen emulsion.         It was

Each of the liquid foundations and sunscreen emulsions obtained in Examples 11 to 14 had excellent stability over time, and when applied to the skin, "sunburn" or spots on the skin due to sunburn, freckles and aging It was a liquid foundation and sunscreen emulsion that prevents wrinkles and tarmi and makes the skin clear and beautiful.

[0130]

EFFECTS OF THE INVENTION The external preparation for skin of the present invention has a melanin production inhibitory effect and an antioxidant effect when it contains a purple cell culture extract, so that it has a high inhibitory effect on pigmentation and an anti-aging effect. By exhibiting the above, it is effective for preventing and improving darkening of the skin due to dullness of the skin, sunburn and the like, prevention and improvement of spots and freckles, and prevention and improvement of wrinkles and tarmi due to aging and ultraviolet rays.

Further, the skin external preparation composition of the present invention, which is prepared by combining the cultured cell extract of Murasaki with other medicinal components such as a whitening agent, an antioxidant, an anti-inflammatory agent, a cell activating agent, and an anti-UV agent. Has a whitening effect, a beautiful skin effect, and an antioxidant and anti-aging effect, which are superior to those obtained by blending the extract alone.

Therefore, the skin external preparation and the skin external preparation composition of the present invention include cosmetics and pharmaceuticals (including external pharmaceuticals) for whitening, beautiful skin and anti-oxidation and anti-aging.
Emulsion, cream, lotion, beauty essence, pack, cleaning agent, makeup cosmetic, dispersion, ointment, liquid agent, aerosol,
It can be advantageously used as a patch or the like. that's all

Front page continuation (51) Int.Cl. ⁷ Identification code FI theme code (reference) A61P 43/00 111 A61P 43/00 111 (72) Inventor Kumi Kameyama 48-18 Sakaemachi, Kita-ku, Tokyo Kose Co., Ltd. (72) Inventor Koichi Matsubara 6-1-2 Waki, Waki-cho, Kuga-gun, Yamaguchi Mitsui Chemicals, Inc. (72) Innovator Shinya Hirokane 6-1-2 Waki, Waki-cho, Yamaguchi Prefecture Mitsui Chemicals Incorporated (72) Inventor Takashi Tadada 1144 Togo, Mobara-shi, Chiba Mitsui Chemicals, Inc. F-term (reference) 4C083 AA032 AA082 AA111 AA112 AB032 AB172 AB212 AB232 AB242 AB332 AB432 AB442 AC022 AC072 AC102 AC122 AC132 AC212 AC242 AC302 AC342 AC352 AC422 AC422 AC432 AC442 AC542 AC582 AC612 AC852 AC912 AD022 AD042 AD112 AD152 AD162 AD172 AD262 AD272 AD342 AD392 AD512 AD532 AD572 AD622 AD642 AD662 BB46 BB47 BB51 CC02 CC04 CC05 CC07 CC12 CC19 DD22 DD23 DD27 DD31 DD41 EE01 BA12 AC06 MA1220 NA14 ZA89 ZB22 ZC02

Claims (5)

[Claims] 1. A whitening and / or whitening of an extract of cultured purple cells
Or a skin external preparation contained as a skin beautifying ingredient. 2. A skin external preparation containing an extract of cultured purple cells as a cell activating component. 3. An external preparation for skin containing an extract of cultured cells of purple purple as a SOD (superoxide dismutase) -like active ingredient. 4. A skin external preparation for aging prevention.
The external preparation for skin according to any one of items 1 to 3. 5. A component selected from the group consisting of the following components (A) and (B) (A) an extract of cultured cells of Murasaki (B) a whitening agent, an antioxidant, an anti-inflammatory agent, a cell activator, and an anti-UV agent. A skin external preparation composition containing one or more of the following medicinal components.