JP2002333443A - Blood-collecting tube - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a blood collecting tube preventing fluidization of a filled thixotropic gel-like separating agent during horizontal storing and capable of forming a partition having a sufficient thickness even when centrifuged by a centrifugal force of 1,000 G or below. SOLUTION: Circumferential ridges 21 are projected intermittently on the inner peripheral wall at immediately above the interface of the thixotropic gel-like separating agent 3 filled in the tube bottom of the blood-collecting tube body 1 having a generally test tube form.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to a blood collection tube.

[0002]

2. Description of the Related Art Conventionally, a tube bottom is filled with a thixotropic gel-like separating agent (ρ = 1.05) having a specific gravity intermediate between that of serum (ρ = 1.02) and blood clot (ρ = 1.08). When blood is collected in the main body of the substantially test tubular blood collection tube and allowed to stand, and then centrifuged, the gel-like separating agent is fluidized so that it is located between the clot and the serum separated from the clot. It is known that the barrier ribs are gelled again to form partition walls.

[0003]

However, in such a blood collection tube, a part of the filled thixotropic gel-like separating agent is fluidized during the horizontal storage, and the filling interface collapses. If the centrifugal separation operation is performed at a centrifugal force of 1,000 G or less so as not to damage the red blood cells and cause hemolysis, the fluidity of the gel separating agent is insufficient, and There is a problem that the formation of the partition walls may be hindered.

[0004] The present invention solves the above-mentioned conventional problems, prevents the filled thixotropic gel-like separating agent from being fluidized during horizontal storage, and performs a centrifugal separation operation with a centrifugal force of 1,000 G or less. It is an object of the present invention to provide a blood collection tube capable of forming a partition wall having a sufficient thickness even when the above method is performed.

[0005]

According to the blood collection tube of the present invention, a circumferential ridge is formed on an inner peripheral wall immediately above an interface of a thixotropic gel-like separating agent filled in a tube bottom of a substantially test tubular blood collection tube main body. It is characterized by being protruded intermittently.

[0006] In the blood collection tube of the present invention, the material of the blood collection tube main body is not particularly limited as long as it does not alter the blood components and has high strength. For example, polyethylene terephthalate (PET), polycarbonate (PC) ), And the like, and glass, etc., and the former, which is hardly damaged by falling or the like, is preferably used.

[0007] The protruding ridge intermittently protruding from the inner peripheral wall of the blood collection tube main body is formed at the lower end of a cylindrical body formed separately from the blood collection tube main body, even if it is integrally formed with the blood collection tube main body. The cylindrical body may be protruded by being inserted into the blood collection tube main body, or may be any.

When the ridge is formed separately from the main body of the blood collection tube, its material is not particularly limited as long as it does not alter blood components and has high strength.
Examples thereof include thermoplastic hard synthetic resins such as T, PC, polypropylene (PP), and high-density polyethylene (HDPE), glass, and the like. Particularly, the former which is hardly damaged by dropping or the like is preferably used.

[0009] The cross-sectional shape of the ridge is not particularly limited as long as it assists the movement of the blood clot, which has started to coagulate and contract, toward the center, and is, for example, a triangular, rectangular, trapezoidal, or the like. Either may be used.

The ridges are preferably equally divided by a cut which becomes a passage through which the gel-like separating agent fluidized by the centrifugal operation moves along the tube wall.
The number may be any of four, four, and the like.

The width of the cut is not particularly limited as long as the fluidized gel-like separating agent can move upward through the cut. For example, when the inner diameter is 15 mm, the width is 0.1 to 5 m.
m is preferable.

The height of the ridge in the radial direction is not particularly limited as long as a circular hole through which a sedimenting blood clot and a floating serum can pass is left at the center. For example, when the inner diameter is 15 mm, ,
It is preferably 0.1 to 7 mm. The vertical width of the ridge is not particularly limited, and is preferably 0.1 to 1 mm.

The position where the ridge is formed is preferably provided in contact with the interface of the filled thixotropic gel-like separating agent. For example, in the case of a 10 cc blood collection tube, it is 5 to 20 mm from the bottom of the tube. Preferably.

The material of the thixotropic gel-like separating agent filled in the tube bottom of the blood collection tube main body is not particularly limited, and conventionally known silicones, silica, and structure-forming agents (α-olefin / ester copolymer / filler, Polybutadiene / filler, epoxidized polybutadiene / filler).

[0015]

Embodiments of the present invention will be described below with reference to the drawings. FIG. 1 is a sectional view showing a first embodiment of a blood collection tube of the present invention, and FIG. 2 is a sectional view showing a second embodiment of a blood collection tube of the present invention.

The first embodiment of the blood collection tube of the present invention shown in FIG.
In the embodiment, a cylindrical body 2 having a substantially test-tube-shaped blood collection tube main body 1 formed with a ridge 21 having a triangular cross-section at the lower end and divided into four equal parts by a cut and having a folded edge 22 extended at an upper end. Is inserted, and a circumferential ridge 21 is intermittently provided on the inner peripheral wall immediately above the interface of the thixotropic gel-like separating agent 3 filled in the tube bottom.

The second embodiment of the blood collection tube of the present invention shown in FIG.
In the embodiment, an inner peripheral wall immediately above the interface of the thixotropic gel-like separating agent 3 filled in the tube bottom of the blood collection tube main body 1 has a circumferential ridge having a rectangular cross section divided into three equal parts by cuts. 11 intermittently protrude integrally.

[Example 1] Inner diameter 11 mm, outer diameter 13 m
m, a substantially test tubular blood collection tube main body 1 made of PET having a length of 100 mm and formed at the lower end with a ridge 21 having a width of 1 mm and a triangular cross section formed by a 1 mm wide cut, and a length of 85 mm.
Of the PP cylindrical body 2 described above, and the specific gravity at 25 ° C. is 1.
A thixotropic gel-like separating agent 3 having a viscosity of 110,000 cp and a weight of 05 g / cm ³ was filled until the interface of the gel-like separating agent 3 came into contact with the ridge 21 so as not to mix bubbles.

Next, a blood coagulation accelerator containing silica as a main component is applied to the inner surface of the blood collection tube main body 1 and sufficiently dried.
A butyl rubber stopper was plugged under a reduced pressure of 30 mmHg to prepare a vacuum blood collection tube.

[Comparative Example] A vacuum blood collection tube was prepared in the same manner as in the embodiment except that a PP cylindrical body having a ridge 21 was inserted into the blood collection tube main body 1 and the ridge 21 was not provided. Created.

Four vacuum blood collection tubes of the above embodiment and five vacuum blood collection tubes of the comparative example were left horizontally at 60 ° C. for 2 days.
The presence / absence of fluidization at the interface of the thixotropic gel-like separating agent and the flow distance when present were measured. Table 1 shows the measurement results.
It is summarized and shown.

[0022]

[Table 1]

As is clear from Table 1, fluidization at the interface of the gel-like separating agent was not observed at all in the blood collection tubes of Examples, but fluidization was observed in some of the blood collection tubes of Comparative Examples. I understand.

Next, 6 cc of blood of a healthy person was collected into four vacuum blood collection tubes of the above-described embodiment and five vacuum blood collection tubes of the comparative example, and left at room temperature for about 3 hours to completely coagulate the blood.
After centrifugation at 1,000 G for 5 minutes, the presence or absence of a partition between the serum and the blood clot and, if present, the thickness of the partition were measured. Table 2 summarizes the measurement results.

[0025]

[Table 2]

As is clear from Table 2, the blood collection tubes of the examples all have a partition wall having a thickness of 5 mm or more. It can be seen that some have a thickness less than.

[0027]

Since the blood collection tube of the present invention is constituted as described above, the filled thixotropic gel-like separating agent is prevented from being fluidized at the time of horizontal storage, and 1,0.
Even when the centrifugal separation operation is performed with a centrifugal force of 00 G or less, a partition wall having a sufficient thickness can be formed.

[Brief description of the drawings]

FIG. 1 is a sectional view showing a first embodiment of a blood collection tube according to the present invention.

FIG. 2 is a sectional view showing a second embodiment of the blood collection tube of the present invention.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 Blood collection tube main body 2 Cylindrical body 3 Gel-like separating agent 11 Protrusion (projected to the blood collection main body) 21 Protrusion (projected to the cylinder) 22 Turned edge

 ──────────────────────────────────────────────────の Continued on the front page F term (reference) 2G045 AA01 BA08 BA10 BB10 CA25 FA05 HA06 HA14 HB13 HB16 HB17 4C038 TA01 UB10 UC10 4D057 AA03 AB01 AC01 AC05 AD01 AE11

Claims (1)

[Claims] The present invention is characterized in that a circumferential ridge is intermittently provided on an inner peripheral wall immediately above an interface of a thixotropic gel-like separating agent filled in a tube bottom of a substantially test tubular blood collection tube main body. And blood collection tube.