JP2002020243A - Agent for preventing and improving gray hair - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a gray hair-preventing and improving agent which has an activity to activate the melanocytes of hair roots and stimulate the production of melanin, when applied to scalp. SOLUTION: This gray hair-preventing and improving agent characterized by containing the extracts of one or more plants selected from Urtica dioica L., Rosa multiflara Thunberg, Valeriana fauriei Briquet, V. officinalis, Cochlearia armoracia L., Aromoracia rusticana GAERTN., A. lapathifolia Gilbert, Camellia japonica L., Euphoria longana, Cyperus rotundus, Crataegus pinnatifida var. major N. E. Brown, C. cuneata Siebold et Zuccarini, C. pinnatifidia Bunge, Aneuxopaegmaarvense, Catuaba, Solanum paniculatum, Jurubeba, Styphnodendron adstringens, Barbatimao-verdadeiro, Dalbergiasubcymosa, Veronica, Aniba canelilla, Casca-preciosa, Pothomorpheumbellata, Caapeba, Pilocarpus jaborandi, Jaborandi, Carapa guianensis, Andiroba, Dipteryxodorata, Cumaru, Simarouba amara, Marupauba, Ficus inspida, Coajinguba, Boerhavia coccinea, Erva-tostao, Guazuma ulmifolia, Mutamba, Heteropteris anceps, and No-de-cachorro.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for externally applying to the scalp to activate melanocytes in hair roots and promote melanin production to prevent or improve gray hair.

[0002]

BACKGROUND OF THE INVENTION Melanocytes present in the upper part of the hair matrix of hair usually play an important role in the darkening of hair by actively producing melanin and transporting it to keratinocytes of the hair cortex cells. Is playing. However, in the case of gray hair, melanocytes present in the upper part of the hair matrix or cells or factors involved in melanin production are damaged for some reason and lose their functions, resulting in hair that has lost melanin, that is, gray hair. Although the direct cause of gray hair has not yet been elucidated, it is generally considered that one of the aging phenomena accompanying aging occurs. As a means for improving these from the viewpoint of beauty, hair dyes have been conventionally used. However,
Such a hair dye has a temporary effect, and causes problems such as troublesome regular use, inflammation of the scalp due to repeated use, and damage to hair. Further, there are problems in use such as color transfer to clothes and skin at the time of hair dyeing, and it was not always satisfactory. Accordingly, there has been a demand for the development of a hair preparation which essentially prevents or improves the generation of gray hair itself.

In response to such a demand, for example, Japanese Patent Application Laid-Open
-275210, 06-172134, 07-
As shown in JP-A-187962 and JP-A-07-316026, there are those containing a plant extract as an active ingredient, and in addition, JP-A-05-221834 and JP-A-06-10042.
No. 3, No. 07-285874, No. 07-267829
No. are disclosed. However, they have not been satisfactory for users in terms of their effectiveness, stability of active ingredients, and safety. Therefore, the present invention
An object of the present invention is to provide an external preparation for hair capable of preventing and improving white hair by externally applying to the scalp to activate melanocytes of hair roots and restore melanin production.

[0004]

Means for Solving the Problems and Embodiments of the Invention The present inventors have conducted intensive studies in order to achieve the above object, and have found that the above extract has an excellent effect on the activation of melanocytes in hair roots. And completed the present invention. That is, according to the present invention, nettle (Urtic)
a dioca L.A. ), Noibara (Rosa mu)
ltifara Thunberg), Valeriana (Valiana fauriei Brique)
t, V. officinalis), horseradish (Cochlearia armorcia L.,
Aromorasia rusticana GAER
TN. , A. lapathifolia Gilber
t), camellia (Camellia japonica)
L. ), Longan (Euphoria longgan)
a), Kobushi (Cyperus rotundu)
s), hawthorn (Crataegus pinnati)
fida var. major N. E. FIG. Brown,
C. cuneata Siebold et Zucc
arini, C.I. pinnatifidia Bung
e), Anemopaegmaarven
se, Catuaba), Julbeba (Solanu)
m paniculatum, Jurubeba, Barbatimmon Verdadeiro (Styphnodend)
ron adsringens, Barbatima
o-verdadeiro), Veronica (Dalbe)
rgiasubcymosa, Veronica), Casca presiosa (Aniba cannell)
a, Casca-preciosa), Cahorpeba (Pothomorpheumbellata, Caa)
peba), Jaborandi (Pilocarpus)
jaborandi, Jaborandi), Andy Rover (Carapa guianensis, And)
iroba), Kumaroo (Dipteryxodora)
ta, Cumaru, Japanese name Tofu bean), Marpauba (Simarouba amara, Marupaub)
a, Japanese name Simulba), Cozy Guamba (Ficus i)
nspida, Coajinguba), Elva Tostone (Boerhavia coccinea, Er)
va-tostao), Mutamba (Guazuma u)
lmifolia, Mutamba), Node Caschoro (Heteropteris ancesps, No)
-De-cachroro), a novel agent for preventing and improving white hair comprising one or more plants selected from the group consisting of:

Hereinafter, the present invention will be described in more detail. As described above, the gray hair preventing / improving agent of the present invention includes nettle (Urtica dioica L.) and neubara (Rosa multiflara Thumber).
g), Valeriana faurie
i Briquet, V .; officinalis),
Horseradish (Cochlearia armora)
cia L. , Aromorasialustican
a GAERTN. , A. lapathifolia
Gilbert), camellia (Camellia jap)
onica L. ), Longan (Euphoria)
longana), Kobushi (Cyperus rot)
undus) and Hawthorn (Crataegus pin)
natifida var. major N. E. FIG. Br
own, C.O. cuneata Siebold et
Zuccarini, C .; pinnatifidia
Bunge), Anatopaegma
arvense, Catuaba), Jurbaeva (S
olanum paniculatum, Jurub
ba), Barbatimmon Verdadeiro (Styphn)
oldron adsringens, Barb
atimao-verdadeiro), Veronica (Dalbergia subcymosa, Vero)
nica), Casca Preciosa (Aniba ca)
nellilla, Casca-preciosa), Caapaver (Pothomorphe umbella)
ta, Caapeba), Jaborandi (Piloc)
arpus jaborandi, jaborand
i), Andy Rover (Carapa guianen)
sis, Andiroba), Kumaroo (Dipter)
yx odorata, Cumaru (Japanese name Tonkameme), Malpauba (Simarouba amar)
a, Marupauba, Japanese name Simulba), Coginguuba (Ficus inspida, Coajingu)
ba), Elva Toston (Boerhaviac)
occinea, Erva-tostao, Mutamba (Guazuma ulmifolia, Mutamb)
a), Heteropteris
ances, No-de-cacherro) and one or more of its extracts as active ingredients. The plant extract may be an extract extract or a product separated and purified from the extract. In the case of an extract extract, it can be obtained by solvent extraction of a dried or crushed plant as described above,
If the extraction solvent is non-toxic for use, the extract may be used as it is, may be used as a diluent diluted with an appropriate solvent, or may be used as a concentrated extract, or as a dry powder by freeze-drying, or paste. And the like prepared in a state can be used. Examples of the solvent used to obtain the plant extract include commonly used organic solvents such as methanol, ethanol, butanol, hexane, heptane, cyclohexane, ethyl acetate, and acetone, and water. The species can be used alone or in combination of two or more. Among these solvents, polar solvents are preferred, and ethanol and water are more preferred.

[0006] The extraction can be carried out by a conventional method at a temperature of usually about 3 to 70 ° C. In addition to the solvent extraction, an extract obtained by supercritical extraction in which carbon dioxide is brought into a supercritical state can be similarly used. At this time, hexane, ethanol, or the like can also be used as an extraction aid. Separation and purification of the active ingredient from the extract can be performed by purifying the extract by column chromatography, liquid chromatography, or the like. The amount of the plant or its extract can be appropriately changed depending on the dosage form and the like, but it is usually preferable to add 0.00001 to 20% (% by mass, the same applies hereinafter) to the total amount of the hair cosmetic. Preferably, the content is 0.0001 to 10%. If the compounding amount is less than 0.00001%, the effects of the present invention cannot be exerted, and the production may be difficult depending on the dosage form. Therefore, it is better not to exceed 20%.

[0007] There are no particular restrictions on the dosage form and the like of the gray hair preventing / improving agent of the present invention. For example, creams, lotions,
It can be formulated and used in various forms such as emulsion, ointment, gel, liniment, hair tonic, hair liquid, hair rinse, hair shampoo, hair treatment, hair conditioner, aerosol, air foam and the like. Further, the liquid form may be used in combination with a usual propellant, carbon dioxide, oxygen gas, LPG or the like, and filled in a pressure-resistant container. The gray hair preventing / improving agent of the present invention may contain various components in addition to the above essential components according to the dosage form and the like. That is, in the present invention,
The above-mentioned plant or its extract is used in combination with other medicinal ingredients, for example, C-AMP and its derivatives which are said to have a gray-hair preventing effect, forskolin, carpronium chloride, glyceride pentadecanoate, minoxidil, bFGF and the like. By doing so, the effect can be enhanced. In addition, for example, vitamin E, vitamin E nicotinate, cepharanthin, nicotinic acid, nicotinamide, benzyl nicotinate, ginger tincture, capsicum tincture, peripheral extract such as assembly extract Vasodilators, coenzyme Q, flavonoids, tannins, antioxidants such as polyphenols, cooling agents such as camphor and 1-menthol, lysozyme chloride, glycyrrhizin,
Anti-inflammatory agents such as allantoin, cell activators such as garlic extract, carrot extract, ogre extract, rosemary extract, aloe extract, placenta extract, DNA synthesis promoters such as 6-benzylaminopurine, vitamin A, vitamin B6, pantothene Acids, vitamins such as biotin, amino acids such as methionine, cysteine, cystine, and tyrosine; bactericides such as salicylic acid, hinokitiol, resorcinol, trichlorocarbanylde, and isopropylmethylphenol; hormones such as ethinyl estradiol and progesterone; Extracts from aloe, jujube fruit, sesame and the like can be blended.

[0008] Further, for hair cosmetics such as oils, surfactants, humectants, lower alcohols, thickeners, antioxidants, chelating agents, sensation imparting agents, pH adjusters, preservatives, fragrances, pigments, etc. The raw materials used can be compounded. Specifically, oils such as olive oil, jojoba oil, oils and fats such as hardened oil, waxes such as spermaceti, beeswax and lanolin, liquid paraffin, ceresin, hydrocarbons such as squalane,
Fatty acids such as stearic acid and oleic acid; alcohols such as cetanol, stearyl alcohol, lanolin alcohol, and hexyldecanol; and esters such as isopropyl mysterylate and butyl stearate. The oil content is preferably 0.5 to 85% in the hair cosmetic. Examples of the surfactant include anionic surfactants such as sodium stearate, sodium cetyl sulfate, polyoxyethylene lauryl ether phosphate, sodium N-acylglutamate, glyceryl monostearate, sorbitan monostearate, and sorbitan sesquioleate. Sucrose fatty acid ester, propylene glycol monostearate, polyoxyethylene oleyl ether, polyoxylauryl ether, polyoxyethylene stearyl ether, polyoxyethylene octyl phenyl ether, polyoxyethylene nonyl phenyl ether, polyethylene glycol monostearate, mono Polyoxyethylene sorbitan palmitate,
Polyoxyethylene coconut fatty acid monoethanolamide,
Polyoxyethylene polyoxypropylene glycol,
Polyoxyethylene castor oil, polyoxyethylene hydrogenated castor oil, polyoxyethylene hydrogenated castor oil isostearate, polyoxyethylene hydrogenated castor oil isostearate, pyroglutamic acid isostearate polyoxyethylene castor oil, polyoxyethylene glyceryl isostearate, triisostearin Examples include nonionic surfactants such as polyoxyethylene glyceryl acid, polyoxyethylene sorbite tetraoleate, and polyoxyethylene lanolinic acid. The surfactant is preferably contained in the hair cosmetic in an amount of 0.1 to 15%.

Further, humectants include glycerin, 1,
3-butylene glycol, propylene glycol, dipropylene glycol, ethylene glycol, and the like; lower alcohols such as ethanol and isopropanol; use sensation agents such as 1-menthol and benzyl nicotinate; , Polyethylene glycol, sodium carboxymethyl cellulose,
As antioxidants, dibutylhydroxytoluene, butylhydroxyanisole, propyl gallate and the like,
As the chelating agent, disodium edetate, ethanehydroxydiphosphate, etc., as the pH adjusting agent,
Citric acid, sodium citrate, boric acid, sodium monohydrogen phosphate, etc., as preservatives, methyl parahydroxybenzoate, ethyl parahydroxybenzoate, dehydroacetic acid,
Salicylic acid, benzoic acid and the like can be exemplified. The optional components are not limited to these.

The amount of the gray hair preventing / improving agent of the present invention can be appropriately changed depending on the dosage form and the like, but it is usually preferable to add 0.00001 to 20% by mass based on the total amount of the external preparation. Preferably, the content is 0.0001 to 10% by mass.
If the amount is less than 0.00001% by mass, the effects of the present invention cannot be exhibited, and the production of some dosage forms becomes difficult. Therefore, it is preferable that the amount does not exceed 20% by mass.

[0011]

The white hair improving / preventing agent provided by the present invention exerts excellent white hair preventing and improving effects when applied externally to the scalp.

[0012]

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described specifically with reference to Preparation Examples, Examples, and Formulation Examples, but the present invention is not limited to the following examples. [Preparation Example 1] 10 times (W / V) of methanol was added to 1 kg of the cut material of the nettle root, and the mixture was extracted for 2 hours. Next, the mixture was filtered, and ethanol was distilled off under reduced pressure.
Obtained. In the same manner, ethanol extract of leaves of wild rose, ethanol extract of valerian root and ethanol extract of horseradish root, water extract of aerial part of camellia, ethanol extract of pulp of longan,
A methanol extract of the bark of Kobushi was obtained.

[Test Example 1] Cell culture method Using a cultured pigment cell (B16 melanoma), nettle, wild rose, valerian, horseradish, camellia,
Longan, Kobushi, Hawthorn, Catourba, Giulbeba, Barbatimon Verdadeiro, Veronica, Casca Preciosa, Caerpeba, Jaborandi, Andy Rova, Kumaroo, Malpauba, Coazinguba, Elva Toston, Mutamba, Node
The effect of cashoro on promoting melanogenesis was tested. B16
The cultivation of melanoma was performed using Eagle's medium containing 10% FBS. Cells were seeded at a density of 0.75 × 10 ⁴ cells / cm ² and placed in a CO ₂ incubator (5% CO ₂ ).
And cultured at 37 ° C. After 24 hours of culture, the sample solution was replaced with the above-mentioned medium containing a final concentration of 10 ⁻² to 10 ⁻⁵ mass%, or only the medium as a control, and further cultured for 72 hours.

Visual measurement of the amount of melanin 72 hours after the addition of the sample solution, the sample solution was removed, washed with a phosphate buffer solution, and the B on the plate was measured with a cell scraper.
16 melanoma cells were detached. The detached cells were collected in a centrifuge tube, centrifuged to precipitate, and the degree of blackening was visually determined in comparison with control cells without drug. The comparison was made according to the following criteria. Table 1 shows the results.
Shown in

[0015] From the above results, nettle extract, radish extract, valerian extract, koucha extract, horseradish extract, camellia extract, longan extract, kobushi extract, hawthorn extract, medusa, and catourba Extracts, Juluva Extracts, Barbatimmon Verdadeiro Extracts, Veronica Extracts, Casca Preciosa Extracts, Caapeva Extracts, Jaborandi Extracts, Andylover Extracts, Kumaroo Extracts, Malpauba Extracts, Coazinguba Extracts, Elva -A strong melanin production-promoting effect was observed in the Tostone extract, Mutamba extract, and Node cashoro extract.

[0016]

[0017]

EXAMPLES The following are examples of nettle extract, wild rose extract, valerian extract, scotch extract, horseradish extract, camellia extract, longan extract, kobushi extract, hawthorn extract, medusa, and catourba extract. Extract, jurubeba extract, barbatimmon verdadeiro extract, veronica extract, casca preciosa extract, caapeba extract, jaborandi extract, andiroba extract, coumaro extract, malpauba extract, coazinguba extract, elva An example of a formulation in which a Tostone extract, a Mutamba extract, and a Node Cascio extract are blended is shown. The following examples all showed excellent white hair prevention / improvement effects, and also had good safety.

[0018]

[0019]

[0020]

[0021]

[0022]

[0023]

[0024]

[0025] The pH of the shampoo described in this example is between 3 and 7.5.
Range.

[0026]

The extract of the present invention may be incorporated into a hair restorer in addition to this product. It can also be applied to face care agents to prevent and improve white hair on beards and eyebrows, and to bath salts to prevent and improve white hair on body hair. In addition, Japanese Patent Application No. 1
By combining with the technology or / and the drug disclosed in 1-220197, it is also possible to exert a synergistic effect on the prevention and improvement of gray hair.

[0028]

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification code FI theme coat ゛ (reference) A61P 43/00 111 A61P 43/00 111 F term (reference) 4C083 AA111 AA112 AA122 AB032 AB242 AB332 AB352 AB442 AC012 AC022 AC072 AC082 AC102 AC112 AC122 AC132 AC182 AC242 AC302 AC312 AC342 AC352 AC392 AC422 AC432 AC442 AC472 AC482 AC532 AC542 AC562 AC582 AC612 AC622 AC642 AC662 AC692 AC712 AC782 AC792 AC812 AC842 AC852 AC892 AD022 AD042 AD092 AD132 AD152 AD162 AD172 AD282 AD472 CC04 CC05 CC23 CC31 CC33 CC38 CC39 DD31 EE24 4C088 AB12 AB15 AB23 AB45 AB51 AB59 AC02 AC04 AC05 AC06 AC11 CA06 MA63 NA14 ZA92

Claims (1)

[Claims] Claims: 1. Nettle, wild rose, valerian, horseradish, camellia, longan, kobushi, hawthorn,
Plants or extracts thereof selected from the group consisting of catourba, jurubeba, barbatimon verdadeiro, veronica, casca preciosa, kaapeba, jaborandi, andiroba, kmaloo, malpauba, coazinguba, elva toston, mutamba, node cashoro. An agent for preventing and / or improving white hair comprising one or more of the following as active ingredients.