JP2002051770A - Protease stabilizer and liquid cosmetic composition - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a protease stabilizer capable of inhibiting/preventing a protease from being deactivated, capable of inhibiting/preventing the protease contained in various cosmetics from being deactivated while the cosmetics are stored by adding the stabilizer to the cosmetics, and further capable of making the protease sufficiently exhibit its enzyme activity when the cosmetics are applied, and to provide a liquid cosmetic composition, such as a shampoo, rinse and body soap, excellent in feelings when used, by adding the stabilizer to the composition. SOLUTION: This protease stabilizer contains one or more kinds of compounds selected from keratin, a keratin derivative, and an albumen peptide as active ingredients. The liquid cosmetic composition contains the protease stabilizer, active ingredients for the cosmetics, and the protease.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a proteolytic enzyme stabilizing agent which suppresses a decrease in the activity of proteolytic enzymes incorporated in liquid cosmetics and the like and stably retains the same, and a shampoo containing the stabilizing agent. The present invention relates to a liquid cosmetic composition which can be used for rinsing, rinsing, body soap and the like.

[0002]

2. Description of the Related Art Various enzyme-containing cosmetics are conventionally known. In general, enzymes are easily deactivated by contact with other components contained in cosmetics or oxidation. Therefore, in the enzyme-containing cosmetics, various methods are used to stabilize various enzymes so that the enzyme is not deactivated during storage and the enzyme activity can be sufficiently exhibited at the time of use. Inactivation of enzyme activity
This is particularly noticeable in liquid products. Therefore, as a method of stabilizing the protease in such a liquid product, a method of blending collagen, a method of blending a reducing agent such as sodium sulfite for preventing oxidation of the protease, and the like have been proposed. However, the effects of these methods cannot be said to be sufficient, and furthermore, when used in cosmetics, there is a problem that the feeling of use is affected.

[0003]

SUMMARY OF THE INVENTION Accordingly, an object of the present invention is to suppress and prevent the inactivation of a proteolytic enzyme, and by incorporating it into various cosmetics, the proteolytic enzyme in the cosmetic can be stored during storage. An object of the present invention is to provide a proteolytic enzyme stabilizing agent which can suppress or prevent inactivation of the proteolytic enzyme, and can sufficiently exhibit the enzymatic activity of the proteolytic enzyme when used. Another object of the present invention is to suppress and prevent the inactivation of the proteolytic enzyme incorporated in the cosmetic during storage, to allow the enzyme activity of the proteolytic enzyme to be sufficiently exhibited at the time of use, and to provide a feeling of use. It is to provide a liquid cosmetic composition which is excellent in water resistance.

[0004]

According to the present invention, one or more selected from the group consisting of keratin, keratin derivatives and egg white peptides, and if necessary, a polyhydric alcohol as an active ingredient. A proteolytic enzyme stabilizer is provided. Further, according to the present invention, there is provided a liquid cosmetic composition comprising a cosmetic active ingredient, a proteolytic enzyme, and the proteolytic enzyme stabilizer.

[0005]

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in more detail. The proteolytic enzyme stabilizer of the present invention contains one or more selected from the group consisting of keratin, keratin derivative and egg white peptide as an active ingredient, and further contains a polyhydric alcohol as needed.

The keratin derivatives include, for example, N- (2-hydroxy-3- (trimethylammonio) chloride
Propyl) hydrolyzed keratin solution, N- (2-hydroxy-3- (coconut oil alkyldimethylammonio) propyl) hydrolyzed keratin solution, N- (2-hydroxy-3 chloride)
-(Stearyldimethylammonio) propyl) hydrolyzed keratin solution, N- (2-hydroxy-3- (3-dihydroxymethylsilyl) propoxy) propyl) hydrolyzed keratin solution or a mixture thereof. They are,
Commercially available products can also be used, for example, trade name “Promois WK-HSIG” (manufactured by Seiwa Kasei Co., Ltd.) and the like.

[0007] Examples of egg white peptides include those obtained by hydrolyzing chicken egg white with an enzyme.
About 00 to 2000 can be used. In addition, commercially available products can be used, and examples thereof include “Egg White Peptide EP-1” and “Egg White Peptide EP-3S” (manufactured by QP Corporation).

The proteolytic enzyme stabilizer of the present invention may contain, in addition to the above-mentioned essential active ingredients, other ingredients within a range that does not affect the action thereof. May be included. Examples of the polyhydric alcohol include ethylene glycol, diethylene glycol, triethylene glycol, polyethylene glycol, propylene glycol, dipropylene glycol, polypropylene glycol, glycerin, diglycerin, polyglycerin, 3-methyl-1,
Examples thereof include 3-butanediol and 1,3-butylene glycol. When the polyhydric alcohol is blended, the blending ratio is one or two or more selected from the group consisting of the keratin, keratin derivative and egg white peptide.
1 to 30 parts by mass is preferable with respect to 00 parts by mass.

[0009] The liquid cosmetic composition of the present invention comprises a cosmetic active ingredient, a proteolytic enzyme, and the above-mentioned proteolytic enzyme stabilizer. The cosmetic active ingredient can be appropriately selected according to the type of the cosmetic. Preferably, there may be mentioned usual components that can be used for shampoo, rinse, body soap and the like.

[0010] As an active ingredient of shampoo, for example,
Anionic surfactant, nonionic surfactant, amphoteric surfactant, cationic surfactant, bactericide, sugar, amino acid, humectant, plant extract, water-soluble polymer, silicon, antioxidant, ultraviolet inhibitor, chelate Agents, pH adjusters, dyes, fragrances, purified water or mixtures thereof.
As an active ingredient of the rinse, for example, a cationic surfactant, a nonionic surfactant, an anionic surfactant, an amphoteric surfactant, a higher alcohol, an oil component, an amino acid derivative,
Examples include hydrolyzed polypeptides, water-soluble polymers, silicones, humectants, plant extracts, antioxidants, UV inhibitors, chelating agents, bactericides, pigments, fragrances, purified water, and mixtures thereof. As an active ingredient of the body soap, for example, anionic surfactants, nonionic surfactants, amphoteric surfactants, bactericides, sugars, amino acids, humectants, plant extracts, antioxidants, ultraviolet inhibitors, chelating agents, pH
Examples include a regulator, a dye, a fragrance, purified water, or a mixture thereof.

In the liquid cosmetic composition of the present invention, the type of proteolytic enzyme is not particularly limited, and can be appropriately selected depending on the type and purpose of the cosmetic. The mixing ratio of the proteolytic enzyme can be appropriately determined depending on the titer of the enzyme and the like.
It can be blended at 1 to 0.5% by mass.

In the liquid cosmetic composition of the present invention, the stabilizing action of the proteolytic enzyme stabilizer increases as the concentration increases, as can be seen from the examples described below. It can be appropriately determined in consideration of the amount of the decomposing enzyme, its potency, the feeling of use of the cosmetic, and the like.

Various cosmetics can be prepared using the liquid cosmetic composition of the present invention so as to contain the above essential components according to a known method.

[0014]

The proteolytic enzyme stabilizer of the present invention comprises:
Since at least one of keratin, keratin derivative and egg white peptide and, if necessary, a polyhydric alcohol are used as active ingredients, when blended in an enzyme-containing cosmetic or the like, the use feeling of the cosmetic can be prevented from lowering and the protein can be effectively used. Inactivation of the degrading enzyme can be suppressed and prevented. Since the liquid cosmetic composition of the present invention contains the proteolytic enzyme stabilizer, liquid cosmetics, particularly shampoos, rinses, body soaps, etc., effectively deactivate the compounded enzyme during storage. In addition to being able to suppress and prevent, it is possible to prevent a decrease in the usability.

[0015]

The present invention will be described in more detail with reference to the following Examples and Comparative Examples, but the present invention is not limited thereto. Examples 1-1 to 1-4 and Comparative Example 1-1 In distilled water, 0.2% by mass of proteolytic enzyme and N- (2
-Hydroxy-3- (3-dihydroxymethylsilyl) propoxy) propyl) hydrolyzed keratin solution (Promois W
K-HSIG) 0.63% by mass (Example 1-1), 1.2
5% by mass (Example 1-2), 1.88% by mass (Example 1
3) and 2.5% by mass (Examples 1-4) were mixed to prepare 2 ml of each sample solution. Further, 0.2% by mass of the protease was dissolved in distilled water, and 2 ml of a sample solution (Comparative Example 1-
1) was prepared. Using each of the obtained sample solutions, the enzyme activity over time and, after 10 days, 19
After 30 days and 30 days, the residual enzyme activity was determined. Table 1 shows the results of the enzyme activity values over time, and Table 2 shows the residual ratio of the enzyme activity.

<Method for Measuring Enzyme Activity Value and Enzyme Activity Residual Rate> First, 2 ml of a sample solution was added to an acetate buffer solution having a pH of 7.5 (1 mM calcium acetate, 100 mM sodium acetate).
M, 100 mM sodium chloride).
1 ml of the obtained diluted solution is heated to 30 ° C., 5 ml of a 0.6% by mass casein solution is added, and the mixture is reacted at 30 ° C. for 10 minutes. Thereafter, 5 ml of a 6N-TCA solution was added to stop the reaction, and the mixture was left at 30 ° C. for 30 minutes. next,
The obtained reaction solution was filtered, 2 ml of the filtrate was added with 5 ml of a 0.55 M sodium carbonate solution and 1 ml of a Folin reagent, left at 30 ° C. for 30 minutes, and the absorbance at a wavelength of 660 nm was measured. From the measured absorbance, the protease activity of each sample was determined using a calibration curve. Also,
From these results, the enzyme activity remaining ratio was determined. In addition, each reagent used above used what was prepared by the following method. Casein solution: dry matter 1.2g accurately weighed casein corresponding, added and stirred to 0.05M-Na ₂ HPO ₄ 160ml, was heated for about 10 minutes in boiling water. Next, after the casein was completely dissolved, the mixture was cooled to room temperature and cooled to 200 m.
and adjusted to pH 7.5 with 1N-HCl to prepare a 0.6% by mass casein solution. 0.05M-Na ₂ HPO ₄ : 7.10 g of anhydrous sodium monohydrogen phosphate was added to distilled water to prepare a 1 liter solution. Acetate buffer (diluted enzyme solution): 0.18 g of calcium acetate,
1.64 g of sodium acetate, 5.85 g of sodium chloride
Was added to distilled water to prepare a 1 liter solution. TCA solution: 18 g of trichloroacetic acid, sodium acetate 1
Distilled water was added to 8 g of 55 ml of 6N-acetic acid to prepare a 1 liter solution. 0.55M-Na ₂ CO _3: Anhydrous sodium carbonate 58.3
Distilled water was added to 0 g to prepare a 1 liter solution.

[0017]

[Table 1]

[0018]

[Table 2]

Examples 2-1 and 2-2, Comparative Examples 2-1 and 2
-4 Instead of N- (2-hydroxy-3- (3-dihydroxymethylsilyl) propoxy) propyl) hydrolyzed keratin solution, egg white peptide (trade name “Egg white peptide EP-
1 "Kewpie Co., Ltd.) (Example 2-1), Egg White Peptide (trade name" Egg White Peptide EP-3 ", Kewpie Co., Ltd.) (Example 2-2), Tradename" Silkgen G Solble S "(Ichimaru) Falcos Co., Ltd.) (Comparative Example 2-1), trade name “Silkgen G Solble KE” (Ichimaru Falcos)
(Comparative Example 2-2), Lecithin (trade name "San Lecithin A" manufactured by Taiyo Kagaku Co., Ltd.) (Comparative Example 2-3) or "Lactoferrin S" (trade name, manufactured by Ichimaru Falcos Co., Ltd.) ) (Comparative Example 2-
Except that 4) was used, a sample solution was prepared and each measurement was performed in the same manner as in Examples 1-1 to 1-4 and Comparative example 1-1. The results are shown in Tables 3 and 4. The enzyme activity was 10
The measurements were taken after day 22 and day 22.

[0020]

[Table 3]

[0021]

[Table 4]

Examples 3-1 to 3-2, Comparative Examples 3-1 to 3-2
-2 5.80 parts by weight of polyoxyethylene lauryl ether sodium sulfate, coconut oil fatty acid diethanolamide 5.
1. 00 parts by mass, coconut oil fatty acid amidopropyl betaine
00 parts by mass, concentrated glycerin 15.00 parts by mass, N- (2
-Hydroxy-3- (3-dihydroxymethylsilyl) propoxy) propyl) 1.00 part by mass of hydrolyzed keratin solution, 0.50 part by mass of O- (2-hydroxy-3- (trimethylammonio) propyl) hydroxyethyl cellulose chloride 0.20 parts by mass of protease and 70.3 of purified water
Using 0 parts by mass, an enzyme-containing shampoo was prepared by an ordinary method (Example 3-1). An enzyme-containing shampoo was prepared in the same manner as in Example 3-1 except that concentrated glycerin was not used and purified water was changed to 85.30 parts by mass (Example 3-2). N-
Example 3- (3-hydroxy-3- (3-dihydroxymethylsilyl) propoxy) propyl) -hydrolyzed keratin solution was not used, and purified water was changed to 71.30 parts by mass.
In the same manner as in Example 1, an enzyme-containing shampoo was prepared (Comparative Example 3-
1). Concentrated glycerin and N- (2-hydroxy-3- (3-
An enzyme-containing shampoo was prepared in the same manner as in Example 3-1 except that dihydroxymethylsilyl) propoxy) propyl) -hydrolyzed keratin solution was not used and purified water was changed to 86.30 parts by mass (Comparative Example 3-2). ). The protease remaining activity in the shampoo after 10 to 59 days at 40 ° C. was measured for the obtained shampoo according to the following method. The measurement was performed in the same manner except that the obtained shampoo was used in place of the sample liquid in Examples 1-1 to 1-4. Table 5 shows the results. The shampoos of Examples 3-1 to 3-2 and the shampoos of Comparative Examples 3-1 to 3-2 were evaluated for use sensation using panels, but no difference in use sensation was observed.

[0023]

[Table 5]

Formulation Example An enzyme-containing body soap and an enzyme-containing rinse were prepared according to the following formula according to a conventional method. <Prescription of body soap containing enzyme> Sodium laurate 12.3% Sodium myristate 5.8% Coconut oil fatty acid diethanolamide 3.2% Coconut oil fatty acid amide propyl betaine 2.8% Concentrated glycerin 7.0% 1,3 -Butylene glycol 4.3% N- (2-hydroxy-3- (3-dihydroxymethylsilyl) propoxy) propyl) hydrolyzed keratin solution 1.0% protease 0.2% ethyl parahydroxybenzoate 0.05% paraoxybenzo Butyl acid 0.05% Purified water Remainder

<Formulation of enzyme-containing rinse> Stearyltrimethylammonium chloride 3.00% Stearyl alcohol 2.50% Cetyl alcohol 2.00% Liquid paraffin 2.00% Octyldodecanol 0.05% Hydroxyethyl cellulose 0.50% Concentration Glycerin 3.00% N- (2-hydroxy-3- (3-dihydroxymethylsilyl) propoxy) propyl) hydrolyzed keratin solution 1.00% Protease 0.20% Ethyl parahydroxybenzoate 0.05% Butyl paraoxybenzoate 0.05% purified water balance

──────────────────────────────────────────────────の Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI theme coat ゛ (Reference) A61K 7/50 A61K 7/50 // C12N 9/48 C12N 9/48 F-term (Reference) 4B050 CC07 HH02 KK11 KK18 KK20 LL04 4C083 AC022 AC072 AC092 AC111 AC112 AC122 AC242 AC482 AC642 AC692 AC712 AD282 AD411 AD412 AD441 AD442 AD472 CC23 CC38 CC39 DD23 EE01

Claims (5)

[Claims] 1. A proteolytic enzyme stabilizer comprising, as an active ingredient, one or more selected from the group consisting of keratin, keratin derivatives and egg white peptides. 2. A keratin derivative comprising N- (2-hydroxy-3- (trimethylammonio) propyl) hydrolyzed keratin solution, N- (2-hydroxy-3- (coconut oil alkyldimethylammonio) propyl chloride ) Hydrolyzed keratin solution, N- (2-hydroxy-3- (stearyldimethylammonio) propyl) hydrolyzed keratin solution, N-
2. The proteolytic enzyme stabilizer according to claim 1, comprising at least one kind of (2-hydroxy-3- (3-dihydroxymethylsilyl) propoxy) propyl) hydrolyzed keratin solution. 3. The proteolytic enzyme stabilizer according to claim 1, further comprising a polyhydric alcohol. 4. A liquid cosmetic composition comprising a cosmetic active ingredient, a proteolytic enzyme, and the proteolytic enzyme stabilizing agent according to any one of claims 1 to 3. 5. The liquid cosmetic composition according to claim 4, wherein the cosmetic active ingredient is selected from the group consisting of an active ingredient of shampoo, an active ingredient of rinse and an active ingredient of body soap.