ITMI972775A1 - KINOLINIC DERIVATIVES PROCEDURE FOR THEIR PREPARATION AND USE AS NK3 RECEPTOR ANTAGONISTS - Google Patents

Description

Description of the industrial invention entitled: "QUINOLINE DERIVATIVES, PROCEDURE FOR THEIR PREPARATION AND USE AS AN ANTAGONIST OF THE NK3 RECEPTOR"

The present invention relates to new compounds, in particular new quinolinic derivatives, processes for the preparation of such compounds, pharmaceutical compositions containing such compounds and the use of such compounds in medicine.

Neurokinin B (NKB), a mammalian peptide, belongs to the Tachykinin (TK) family of peptides, which also includes Substance P (SP) and Neurokinin A (NKA). Pharmacological and molecular biology tests have shown the existence of three subtypes of the TK receptor (NR · ^ NK2 and NK3); NKB binds preferably to the NK3 receptor, although it also recognizes the other two receptors, with less affinity (Maggi et al., 1993, J. Auton. Pharmacol., 13, 23-93).

Selective peptide antagonists of the NK3 receptor are known (Drapeau, 1990 Regul. Pept., 31, 125-135), and the evidence on peptide agonists of the NK3 receptor suggests that NKB, by activating the NK3 receptor, plays a fundamental role in the modulation of neuronal input at the level of the respiratory tract, skin, spine and nigro-striatal tract (Myers and Undem, 1993, J. Phisiol., 470, 665-679; Counture et al., 1993, Regul. Peptides, 46 , 426-429; Mccarson and Krause, 1994, J. Neurosci., 14 (2), 712-720; Arenas et al., 1991, J.

Neurosci. , 11, 2332-8). However, the peptide-like nature of the known antagonists probably makes them too labile, from the metabolic point of view, to serve as therapeutic agents in practice.

We have now found a new class of non-peptide NK3 receptor antagonists, far more metabolically stable than the known peptide NK3 receptor antagonists and of potential therapeutic utility.

These compounds also possess NK2 antagonist activity and consequently should be considered of potential use in the prevention and treatment of a wide variety of clinical conditions characterized by hyperstimulation of tachykinin receptors, particularly NK3 and NK2.

These conditions include respiratory diseases, such as chronic obstructive pulmonary disease (MPCO), asthma, hyperreactivity of the airways, cough; inflammatory diseases such as inflammatory bowel disease, psoriasis, fibrositis, osteoarthritis, rheumatcid arthritis and pain caused by inflammation; neurogenic inflammation or peripheral neuropathy, allergies such as eczema and rhinitis; ophthalmic disorders such as eye inflammation, conjunctivitis, spring conjunctivitis and the like; skin diseases, skin disorders and itching, such as skin wheals and redness, contact dermatitis, atopic dermatitis, hives and other eczematoid dermatitis; adverse immunological reactions such as rejection of transplanted tissues and disorders related to immune elevation or suppression such as systemic lupus erythematosus; gastrointestinal disorders and diseases (Gl) of the Gl tract such as disorders associated with neuronal control of the viscera, such as ulcerative colitis, Crohn's disease and urinary incontinence; kidney disorders and bladder function disorders (hereinafter referred to as "Primary Conditions").

Some of these compounds also exhibit CNS activity and are therefore considered to be of specific use in the treatment of central nervous system disorders, such as anxiety, depression, psychosis and schizophrenia; neurodegenerative diseases such as AIDS-related dementia, Alzheimer's-type senile dementia, Alzheimer's disease, Down's syndrome, Huntington's disease, Parkinson's disease, movement disorders and seizure disorders (eg epilepsy); demyelinating diseases such as multiple sclerosis and amyotrophic lateral sclerosis, and other neuropathological diseases such as diabetic neuropathy, AIDS-related neuropathy, chemotherapy induced neuropathy and neuralgia; addictive diseases such as alcoholism; somatic disorders related to stress; reflex sympathetic dystrophy, such as spaila / hand syndrome; dysthymic disorders; eating disorders (such as disturbances in food intake); diseases characterized by fibrosis and collagenopathies, such as scleroderma and eosinophilic fascioliasis; blood flow disorders caused by vasodilation and vasospastic diseases such as angina, migraine and Reynaud's disease and pain or pain perception attributable or associated with, for example, any of the foregoing conditions, particularly the transmission of pain in migraine (d ' hereinafter referred to as "Secondary Conditions").

The compounds of formula (I) are also considered useful as diagnostic tools for assessing the degree to which neurokinin-3 and neurokinin-2 receptor activity (normal, hyperactivity or hypoactivity) is involved in a patient's symptoms.

According to the present invention, a compound, or a solvate or salt thereof, of formula (I) is provided

(THE)

in which

Ar is a possibly substituted aryl or group

cycloalkadienyl, or a group or an aromatic heterocyclic group with a single or fused ring, optionally substituted;

R.

is, phenyl or

optionally substituted phenyl, a five-membered heteroaromatic ring optionally substituted, comprising up to four heteroatoms selected from 0 to N, hydroxy amino

or 3, a group forming a ring with a carbon atom of Ar; represents hydrogen or up to four optional substituents selected from the including list:

alkoxy, hydroxy, halogen, nitro, cyano, carboxy, carboxamido, sulfonamido, trifluoromethyl, acyloxy, phthalimido, amino or mono- and d

R2 represents a residue - ^ 1 n is an integer in the interval between 1 and 9, Y ^ and Y2 are independently selected from hydrogen; substituted with hydroxy, 3⁄4_3⁄4-alkylamino or bis j ^ aryl or arii

or Y-j and Y2 together with the nitrogen atom at which they are

bonded represent a heterocyclic group with single or fused ring, optionally substituted, N-bonded;

R3 is branched or linear,

cycloalkylalkyl, optionally substituted aryl, or an aromatic heterocyclic group with single or fused ring, optionally substituted; And

R4 represents hydrogen or

Conveniently, Ar represents optionally substituted phenyl, preferably unsubstituted phenyl.

Conveniently, R represents C, for example ethyl-or iso-propyl.

Conveniently, R ^ represents hydrogen.

Conveniently, it represents an N-linked, possibly substituted, single or fused ring heterocyclic group.

Suitable N-linked single or fused ring heterocyclic groups comprise groups in which any single or fused ring is saturated or unsaturated and comprises 5 or 6 ring atoms, said ring atoms possibly comprising 1 or 2 additional heteroatoms selected from 0 or N, and in which one or two ring atoms are optionally substituted with one or two oxo groups or with one or two of hydroxy, carboxy, C3 -alkoxycarbonyl, aryl, arylalkyl,

or a single ring aromatic heterocyclic group

or melt, or the substituents on adjacent ring atoms form a carbocyclic ring; said aryl or aromatic heterocyclic groups being optionally substituted with one or two C6-alkyl, alkoxy, hydroxy, halogen or halogenalkyl groups.

Fused heterocyclic groups include bonds having one or more rings sharing one or more atoms, such as fused spiral rings, or one or more bonds.

A suitable N-linked single ring heterocyclic group comprising a saturated 5-membered heterocyclic ring is a pyrrolidine-1-yl group.

A suitable N-bonded single ring heterocyclic group comprising a 6-membered saturated heterocyclic ring is a piperidine-1-yl group.

A suitable N-linked single-ring 6-membered saturated heterocyclic group comprising an additional heteroatom is a piperazinyl group, for example an optionally substituted 4-phenylpiperazinyl group.

A suitable N-linked fused ring heterocyclic group comprises a 5 or 6 membered unsaturated or saturated heterocyclic ring fused to a benzene ring.

A suitable N-bonded fused ring heterocyclic group comprising a 6-membered saturated heterocyclic ring fused to a benzene ring is a 2- (1,2,3,4-tetrahydro) isoquinolinyl group.

Suitable N-linked fused heterocyclic groups include fused spiro groups, for example the 1,4-dioxa-8-azaspiro [4.5] decan-8-yl group.

An example of a fused spiro heterocyclic group is an 8-azaspiro [4.5] decan-8-yl group.

A preferred value of is a piperazinyl group, particularly a 4-phenylpiperazinyl.

Preferred compounds of formula (I) are those in which:

Ar is phenyl, R is ethyl, is hydrogen, R2 is a residue

is a piperazinyl label,

especially a 4-phenylpiperazinyl, R3 is phenyl and R4 is hydrogen; in particular, the compounds of Examples 12, 13 and 14 should be mentioned.

The compounds of formula (I) can have at least one center of asymmetry - for example the carbon atom marked with an asterisk (*) in the compound of formula (I) - and therefore can exist in more than one stereoisomeric form. The invention extends to all such stereoisomeric forms and their mixtures, including racemes. In particular, the invention comprises compounds in which the carbon atom with asterisk in formula (I) has the stereochemistry shown in formula (la):

(la) where Ar, R, R ^, R2, R3 and R4 are as defined in relation to formula (I).

The compounds of formula (I) or their salts or solvates are preferably in pharmaceutically acceptable or substantially pure form. By pharmaceutically acceptable form is meant, inter alia, having a pharmaceutically acceptable level of purity, with the exclusion of normal pharmaceutical additives, such as diluents and carriers, and not including material considered toxic at normal dosage levels.

A substantially pure form will generally contain at least 50% (excluding normal pharmaceutical additives), preferably 75%, preferably 90% and even better 95% of the compound of formula (I), or of a salt or solvate thereof. .

A preferred pharmaceutically acceptable form is the crystalline form, including that form in a pharmaceutical composition. In the case of salts and solvates, the ionic portions and additional solvents must also be non-toxic.

Suitable salts are pharmaceutically acceptable salts.

Suitable pharmaceutically acceptable salts include the addition salts with conventional pharmaceutical acids, for example maleic, hydrochloric, hydrobromic, phosphoric, acetic, fumaric, salicylic, citric, lactic, mandelic, tartaric, succinic, benzoic, ascorbic and methanesulfonic acids.

Pharmaceutically suitable salts include the salts of acid portions of the compounds of formula (I), if these are present, for example the salts of carboxy groups or of hydroxy phenolic groups.

Suitable salts of acid moieties include metal salts, such as aluminum salts, alkali metal salts such as lithium, sodium or potassium, alkaline earth metal salts such as calcium or magnesium and substituted ammonium or ammonium salts, for example those with lower alkylamines such as triethylamine, hydroxyalkylamines such as 2-hydroxyethylamine, bis- (2-hydroxyethyl) amine or tri- (2-hydroxyethyl) -amine, cycloalkylamines such as dicyclohexylamine, or with procaine, dibenzylpiperidine, N-benzyl-p-phenethylamine, , Ν, Ν'-didehydroabietylamine, glucamine, N-methylglucamine or pyridine type bases such as pyridine, collidine, quinine or quinoline.

Suitable solvates are pharmaceutically acceptable solvates.

Suitable pharmaceutically acceptable solvates include hydrates.

The term "alkyl" (unless otherwise indicated) when used alone or when belonging to other groups (such as the "alkoxy" group) includes straight or branched chain alkyl groups containing 1 to 12 carbon atoms, suitably 1 6 carbon atoms, and examples include the methyl, ethyl, n-propyl, isopropyl, nbutyl, isobutyl or tert-butyl group.

The term "carbocyclic" refers to cycloalkyl and aryl rings.

The term "cycloalkyl" includes groups having from 3 to 12 ring carbon atoms, suitably from 4 to 6.

The term "curyl" includes phenyl and naphthyl, preferably phenyl, which, unless otherwise indicated, optionally comprises up to five substituents, preferably up to three, selected from halogen, alkyl, phenyl, alkoxy, haloalkyl, hydroxyalkyl, hydroxy groups , amino, nitro, cyano, carboxy, alkoxycarbonyl, alkoxycarbonylalkyl, alkylcarbonyloxy or alkylcarbonyl.

The term "aromatic heterocyclic group" includes groups comprising heterocyclic rings containing from 5 to 12 ring atoms, suitably 5 or 6, and comprising up to four heteroatoms in or each ring selected from S, 0, or N.

Unless otherwise indicated, suitable substituents for any heterocyclic group include up to 4 substituents selected from the group comprising: alkyl, alkoxy, aryl and halogen, or any two substituents on adjacent carbon atoms, together with the carbon atoms to which they are bonded, they can form a curyl group, preferably a benzene ring, and in which the carbon atoms of the aryl group represented by the two aforementioned substituents can themselves be substituted or unsubstituted.

When used herein the term "halogen" refers to fluorine, chlorine, bromine and iodine, preferably fluorine or chlorine.

When used herein, the term "acyl" includes acid residues, particularly a residue of a carboxylic acid such as an alkyl- or aricarbonyl group.

The invention also provides a process for the preparation of a compound of formula (I), or of its salt and / or solvate, which includes the reaction of a compound of formula (II) or of an activated derivative:

(II)

in which they are respectively Rj_, R2 and R3 as defined in relation to the formula {I) or a group convertible respectively into with a compound of formula (III):

(III)

wherein R ', R'4 and Ar' are R, R4 and Ar respectively as defined by the formula (I) or a group or atom convertible into R, R4 and Ar respectively; so as to form a compound of formula (Ib)

(Ib)

in which they are as defined above, and subsequently carrying out one or more of the following optional steps: --( i) converting any one of into respectively, as necessary, so as to obtain a compound of formula (I);

(ii) conversion of compound of formula (I) into another compound of formula (I); And

(iii) preparation of a salt of the compound of formula (I) and / or of a solvate thereof.

Suitable groups convertible to other groups include the protected forms of such groups.

They conveniently represent each respectively or a protected form thereof.

It is preferred that the compound of formula (II) be present as an activated derivative.

A suitable activated derivative of a compound of formula (II) is a transient activated form of the compound of formula (II) or a derivative in which the carboxy group of the compound of formula (II) has been replaced by a different group or atom, for for example from an acyl halide, preferably a chloride, or an acylazide or a carboxylic acid anhydride.

Other suitable active derivatives include: a mixed anhydride formed between the carboxylic residue of the compound of formula (II) and an alkyl chloroformate; an activated ester, such as a cyanomethyl ester, thiophenyl ester, p-nitrophenyl ester, p-nitrothiophenyl ester, 2,4,6-trichlorophenyl ester, pentachlorophenyl ester, pentafluorophenyl ester, N-hydroxyphthalimidoester, N-hydroxypiperidinoester, N-hydroxyzuccinimidoester, N-hydroxyzuccinimidoester; alternatively, the carboxy group of the compound of formula (II) can be activated using a carbodiimide or Ν, Ν'-carbonyldiimidazole.

The reaction between the compound of formula (II) or its activated derivative and the compound of formula (III) is carried out under the conventional conditions suitable for the particular compounds selected. In general, when the compound of formula (II) is present as an activated derivative, the reaction is carried out using the same solvent and conditions as those used to prepare the activated derivative, preferably the activated derivative is prepared in situ before forming the compound of formula (Ib) and subsequently the compound of formula (I) or a salt and / or solvate thereof is prepared.

For example, the reaction between an activated derivative of the compound of formula (II) and the compound of formula (III) can be carried out:

(a) by first preparing an acyl chloride and then reacting said chloride with the compound of formula (III) in the presence of an inorganic or organic base, in a suitable aprotic solvent such as dimethylformamide (DMF) at a temperature in the range of -70 at 50 ° C (preferably in a range from -10 to 20 ° C); or

(b) treating the compound of formula (II) with a compound of formula (III) in the presence of a suitable condensing agent, such as, Ν'-carbonyldiimidazole (CDI) or a carbodiimide, such as dicyclohexylcarbodiimide (DCC) or N -dimethylaminopropyl-N'-ethylcarbodiimide, preferably in the presence of N-hydroxybenzotriazole (HOBT) to maximize yields and avoid racemization processes (see Synthesis, 453, 1972), in an aprotic solvent, such as a mixture of acetonitrile (MeCN) and tetrahydrofuran (THF), for example mixed in a volumetric ratio of 1: 9 to 7: 3 (MeCN: THF), at any temperature that provides an appropriate rate of formation of the required product, such as a temperature in the range from -70 to 50'C, preferably in a range of -10 to 25 "C, for example 0" C.

A preferred reaction is schematized in Scheme 1 shown below:

Scheme 1

where they are as defined above.

It will be noted that a compound of formula (Ib) can be converted into a compound of formula (I), or that a compound of formula (I) can be converted into another compound of formula (I), by interconversion of suitable substituents. Therefore, certain compounds of formula (I) and (Ib) are useful intermediates in the formation of other compounds of the present invention.

Consequently, in a further aspect, the invention provides a process for the preparation of a compound of formula (I), or of a salt and / or a solvate thereof, a process which comprises the conversion of a compound of the formula (Ib ) defined above, in which at least one of

respectively, thereby providing a compound of formula (I); and subsequently, if necessary, the realization of one or more of the following optional stages:

(i) conversion of a compound of formula (I) into another compound of formula (I); And

(ii) preparation of a salt of the compound of formula (I) and / or of a solvate thereof.

Conveniently, in the compound of formula (Ib), the variables Ar ', R',

respectively or

they are protected forms.

The conversions, protections and deprotections mentioned above are carried out using conventional conditions and reagents and are discussed further below.

A compound of formula (II) in which n is the integer 1, is prepared by reacting a compound of formula (IV):

(IV)

wherein R'i and R'3 are as defined above and represents a halogen atom, such as a bromine atom, with a compound of formula (V):

(V) in which they are respectively Y ^ and Y2 as defined in relation to the formula (I) or their protected forms.

Conveniently, they are Y ^ and Y2.

Conveniently, the reaction between the compounds of the formulas (IV) and (-V) is carried out under conventional amination conditions, for example when 3⁄4 is a bromine atom the reaction is then conveniently carried out in an aprotic solvent, such as tetrahydrofuran or dimethylformamide, at any temperature that provides a suitable rate of formation of the desired product, in particular at room temperature; preferably the reaction is carried out in the presence of triethylamine (TEA).

A compound of formula (IV) is prepared by appropriate alienation of a compound of formula (VI):

(YOU)

wherein R'i and R'3 are as defined above in relation to formula (II).

Suitable halogenation reagents are conventional reagents, depending on the nature of the halogen atom required, for example when it is bromine, a preferred halogenation reagent is N-bromosuccinimide (NBS).

The halogenation of the compound of formula (VI) is carried out under conventional conditions, for example the bromination is carried out by treatment with NBS in an inert solvent, such as 1,2-dichloroethane, at any temperature that provides a suitable rate of formation of the required product, suitably at an elevated temperature, such as a temperature in the range from 60'C to 100 "C, for example 80'C, preferably the reaction is carried out in the presence of a catalytic amount of benzoyl peroxide.

A compound of formula (II) where R2 represents

it is conveniently prepared by reaction of a compound of formula (VII):

(VII)

in which R’i is as defined in relation to formula (II), with a compound of formula (VIII):

(Vm)

where R ^ is as defined in relation to formula (II), and Y2 is as defined in relation to formula (I), and p is an integer in the range 2 to 9.

The reaction between the compounds of formula (VII) and (VIII) is conveniently carried out using Pfitzinger reaction conditions (see for example J. Prakt. Chem. 33, 100 (1886), J. Prakt. Chem, 38, 582 ( 1888), J. Chem. Soc. 106 (1948) and Chem. Rev. 35, 152 (1944), for example in an alcoholic solvent such as ethanol, at any temperature that provides an appropriate rate of formation of the required product. , but generally at an elevated temperature, such as at the reflux temperature of the solvent, and preferably in the presence of a base such as potassium hydroxide or potassium tert-butoxide.

A compound of formula (VIII) is prepared from a compound of formula (IX):

(IX)

wherein R'3 is as defined in relation to formula (II) and p is as defined in relation to formula (VIII), first by halogenation, preferably bromination or mesylation, of the compound of formula (IX) and subsequently by reaction of the product resulting halogenation or mesylation with a compound of the formula (V) defined above.

The halogenation of the compound of formula (IX) is suitably carried out using a conventional halogenation reagent. Mesylation is conveniently carried out using mesyl chloride in an inert solvent such as methylene dichloride, at a temperature below room temperature, such as 0'C, preferably in the presence of triethylamine.

The reaction between the halogenation or mesylation product of the compound of formula (IX) and the compound of formula (V) is carried out under conditions similar to those described for the reaction between the compounds of formulas (IV) and (V).

A compound of formula (IX) can be prepared by reaction of a compound of formula (X):

(X)

where p is as defined in relation to formula (VIII), with a lithium salt of formula (XI):

(XI) where R'3 is as defined in relation to formula (II).

The reaction between the compounds of formulas (X) and (XI) can be carried out in an aprotic solvent, such as diethyl ether, at any temperature that provides an appropriate rate of formation of the required product, generally at a low temperature, such as in the range from - 10 ° C to -30 ° C, for example -20 “C.

The compounds of formula (III) are commercially available known compounds and can be prepared from known compounds by known methods, or by methods analogous to those used to prepare known compounds, for example the methods described in Liebigs Ann.der Chemie, 523, 199 , 1936. The compounds of formula (V) are commercially available known compounds or can be prepared using methods analogous to those used to prepare known compounds; for example the methods described in Chemistry of the Amino Group, Patais (Ed.), InterScience, New York 1968 or in Advanced Organic Chemistry, March J. John Wiley & Sons, New York, 1992.

The compounds of formula (VII) are known compounds or can be prepared according to methods used to prepare known compounds, for example those described in J. Org.Chem. 21, 171 (1955): J. Org.Chem. 21, 169 (1955).

The compounds of formula (X) and (XI) are known compounds, or they can be prepared according to methods used to prepare known compounds, for example those described by Krow G. R. in Organic Reactions, Vol. 43, page 251, John Wiley & Sons Ine . 1994 (for compounds of formula (X)) and Organometallic in Synthesis, Schlosser M. (Ed), John Wiley & Sons Ine. 1994 (for compounds of formula (XI)).

As mentioned above, the compounds of formula (I) can exist in more than one stereoisomer form and the process of the invention can produce racemes as well as enantiomerically pure forms. Consequently, an enantiomerically pure form of a compound of formula (I) is obtained by reacting a compound of formula (II) defined above with an appropriate enantiomerically pure primary amine of formula (Illa) or (IIIc):

in which R ', R'4 and Ar' are as defined above, so as to obtain a compound of formula (I'a) or {I'c):

in which they are as defined above.

The compounds of formula (I * a) or (I'c) can subsequently be converted into the compounds of formula (la) or (le) with the conversion methods mentioned above:

in which they are as defined above.

Conveniently, in the above-mentioned compounds of formula (la), (le), (I'a), (I'c), (Ill'a) and (III'c), R4 represents hydrogen.

An alternative method for the separation of optical isomers, for example for those compounds of formula (I) in which R4 is different from hydrogen, consists in the use of conventional methods of separation by fractionation, in particular methods of fractional crystallization.

Thus, a pure enantiomer of a compound of formula (I) is obtained by fractional crystallization of a diastereomer salt formed by reaction of the racemic compound of formula (I) with an optically active strongly acid resolving agent, such as campphosulfonic acid, in an appropriate alcoholic solvent, such as ethanol or methanol, or in a ketone solvent, such as acetone. The salt formation process should be carried out at a temperature between 20 “C and 80 ° C, preferably at 50“ C.

In the event that other basic functions are present in the molecule, such as a primary, secondary or tertiary amine, a wide range of optically active acid resolving agents become available, including tartaric acid, Ο, Ο'-di-p- acid. toluoyltartaric and mandelic acid.

A suitable conversion of a compound of formula (I) into another compound of formula (I) involves the conversion of a group! 3⁄4 into another group! 3⁄4, for example by:

(i) conversion of a ketal to a ketone, by conditions of mild acid hydrolysis, using for example dilute hydrochloric acid; (ii) reduction of a ketone to hydroxyl group, using a reducing agent borohydride;

(iii) converting a carboxylic ester group to a carboxylic group using basic hydrolysis; and / or

(iv) reduction of a methyl ester group to hydroxymethyl group, with the use of a reducing agent borohydride.

As indicated above, where necessary, the conversion of any group which, as indicated above, are usually protected forms of

R4, can be accomplished using suitable conventional conditions, such as the appropriate deprotection procedure.

It will be understood that in each of the aforementioned reactions any activated group in the substrate molecule can be protected and deprotected according to traditional chemical practice, for example as described by Greene, T.W. and Wuts, P.G.M. Protective Groups in Organic Synthesis, John Wiley & Sons Ine. New York, 1991 (Second Edition) or in Kocienski, P.J. Protecting groups, George Thieme Verlag, New York, 1994.

Suitable protecting groups in any of the aforementioned reactions are those conventionally used in the art. Thus, for example, suitable hydroxyl protecting groups include benzyl or trialkylsilyl groups.

The methods of formation and removal of such protecting groups are those conventional methods appropriate for the molecule being protected. Thus, for example, a benzyloxy group can be prepared by treating the suitable compound with a benzyl halide, such as benzyl bromide, and subsequently, if required, the benzyl group can be conveniently eliminated using catalytic hydrogenation or a mild cleavage reagent of the ether, such as trimethylsilyliliodide or boron tribromide.

As indicated above, the compounds of formula (I) have useful pharmaceutical properties, and consequently the present invention also provides a compound of formula (I), or a pharmaceutically acceptable salt or solvate thereof, for use as an active therapeutic substance. The present invention further provides a pharmaceutical composition comprising a compound of formula (I), or a pharmaceutically acceptable salt or solvate thereof, and a pharmaceutically acceptable carrier.

The present invention also provides the use of a compound of formula {!), Or a pharmaceutically acceptable salt or solvate thereof, in the manufacture of a medicament for the treatment of Primary and Secondary Conditions.

This medicament, and a composition of the invention, can be prepared by mixing a compound of the invention with a suitable vehicle. It may contain a diluent, binder, filler, disintegrant, flavoring agent, coloring agent, lubricant or preservative in a conventional manner.

These conventional excipients can be employed, for example, as in the preparation of compositions of known agents, for the treatment of conditions.

Preferably, a pharmaceutical composition of the invention is in unit dosage form and in a form suitable for use in the medical or veterinary field. For example, such preparations may be in packaged form accompanied by written or printed instructions for use as an agent in the treatment of conditions.

The suitable dosage range for the compounds of the invention depends on the compound to be employed and the condition of the patient. It will also depend, among other things, on the relationship between potency and absorbability, on the frequency and route of administration.

The compound or composition of the invention can be formulated for any route of administration and is preferably in unit dosage form or in a form such that a human patient can self-administer it in a single dosage. Advantageously, the composition is suitable for oral, rectal, topical, parenteral, intravenous or intramuscular administration. The preparations can be formulated to give a slow release of the active ingredient.

The compositions can be, for example, in the form of tablets, capsules, sachets, ampoules, powders, granules, tablets, reconstitutable powders, or liquid preparations, for example solutions or suspensions, or suppositories.

The compositions, for example those suitable for oral administration, may contain conventional excipients such as binding agents, for example syrup, acacia, gelatin, sorbitol, tragacanth, or polyvinylpyrrolidone; fillers, for example lactose, sugar, corn starch, calcium phosphate, sorbitol or glyein; compression lubricants, for example magnesium stearate; disintegrants, for example starch, polyvinylpyrrolidone, sodium starch glycolate or microcrystalline cellulose; or pharmaceutically acceptable curing agents such as sodium lauryl sulfate.

The solid compositions can be obtained by conventional methods of mixing, filling, compressing or the like. Repeated mixing operations can be used to distribute the active ingredient in those compositions which employ large amounts of fillers. When the composition is in the form of a tablet, powder or lozenge, any vehicle suitable for formulating solid pharmaceutical compositions can be used, for example magnesium stearate, starch, glucose, lactose, sucrose, rice flour and gypsum. The tablets can be coated according to methods known in normal pharmaceutical practice, in particular with gastro-resistant coatings. The composition may also be in the form of a capsule to be swallowed, for example of gelatin containing the compound, if desired with a vehicle or other excipients.

The liquid compositions for oral administration can be in the form, for example, of emulsions, syrups or elixirs, or they can be presented as a dry product to be reconstituted with water or other suitable vehicle before use. Such liquid compositions can contain conventional additives such as suspending agents, for example sorbitol, syrup, methylcellulose, gelatin, hydroxyethylcellulose, carboxymethylcellulose, aluminum stearate gel, hydrogenated edible fats; emulsifying agents, for example lecithin, sorbitan monooleate, or acacia gum; aqueous or non-aqueous vehicles, which include edible oils, for example, almond oil, fractionated coconut oil, oily esters, for example, glycerin esters, or propylene glycol, or ethyl alcohol, glycerin, water or saline; preservatives, for example methyl or propyl p-hydroxybenzoate or sorbic acid; and, if desired, conventional flavoring or coloring agents.

The compounds of this invention can also be administered via a non-oral route. According to the usual pharmaceutical procedure, the compositions can be formulated, for example, for rectal administration as a suppository. They may also be formulated, for presentation in injectable form, in an aqueous or non-aqueous solution, suspension or emulsion, in a pharmaceutically acceptable liquid, e.g. sterile pyrogenic water or acceptable oil for parenteral administration or a mixture of liquids. The liquid may contain bacteriostatic agents, antioxidants or other preservatives, buffers or solutes to make the solution isotonic with blood, thickening agents, suspending agents or other pharmaceutically acceptable additives. Such forms will be presented in unit dosage form such as ampoules or disposable injection devices or in multi-dose forms such as bottles, from which the appropriate dose can be taken, or a solid or concentrated form that can be used to prepare an injectable formulation.

The compounds of this invention can also be administered by inhalation, through the nasal or oral route. This administration can be carried out with a spray formulation comprising -a compound and a suitable vehicle, optionally suspended for example in a hydrocarbon propellant.

Preferred spray formulations include micronized compound particles in combination with a surfactant, solvent or dispersing agent to prevent sedimentation of the suspended particles. Preferably, the particle size of the compound is from about 2 to 10 microns.

A further method of administering the compounds of the invention comprises transdermal delivery using a formulation in the form of a skin patch. A preferred formulation comprises a compound dispersed in a pressure sensitive adhesive which adheres to the skin, thereby allowing the compound to diffuse from the adhesive through the skin to be delivered to the patient. For a constant rate of percutaneous absorption, pressure sensitive adhesives known in the art, such as natural rubber or silicone, can be used.

As mentioned above, the effective dose of compound depends on the particular compound employed, the patient's condition and the frequency and route of administration. A unit dose will generally contain from 20 to 1000 mg and preferably will contain from 30 to 500 mg, in particular 50, 100, 150, 200, 250, 300, 350, 400, 450 or 500 mg. The conposition can be administered one or more times a day, for example 2, 3 or 4 times a day, and the total daily dose for a 70 kg adult will normally be in the range of 100 to 3000 mg. Alternatively, the unit dose will contain from 2 to 20 mg of active ingredient and will be administered in multiple doses, if desired, to give the above daily dose.

If the compounds are administered according to the invention, no unacceptable toxicological effects are expected.

The present invention also provides a method for the treatment and / or prophylaxis of Primary and Secondary Conditions in mammals, particularly in humans, which includes administering an effective amount of a compound of formula (I) or a pharmaceutically acceptable salt or solvate thereof.

The activity of the compounds of the present invention, as ligands of the NK3 receptor, is determined by their ability to inhibit the binding of the radiolabeled ligands of the receptor.

Senktide, to guinea pig and human NK3 receptors (Renzetti et al., 1991, Neuropeptide, 18, 104-114; Buell et al., 1992, FEBS, 299 (1), 90-95; Chung et al, 1994, Biochem . Biophys. Res. Coimun., 198 (3), 967-972).

The binding tests used allow the determination of the concentration of the single compound necessary to reduce the specific binding of the NK3 receptor by 50% in equilibrium conditions (IC50).

The binding tests provide for each compound tested an average value of IC50 of 2-5 separate experiments performed in duplicate or in triplicate. The most potent compounds of the present invention show IC50 values in the range of 0.1-1000 nM. The NK3-antagonist activity of the compounds of the present invention is determined by their ability to inhibit the contraction of the guinea pig ileus induced by Senktide (Maggi et al., 1990, Br. J. Pharmacol., 101, 996-1000) and of the muscle isolated rabbit iris sphincter (Hall et al., 1991, Eur. J. Pharmacol., 199, 9-14) and human NK3 receptor-mediated Ca ++ mobilization (Mochizuki et al., 1994, J. Biol. Chem., 269, 9651-9658). In vitro functional tests on guinea pig and rabbit provide for each compound tested an average KB value of 3-8 separate experiments, where KB is the concentration of the individual compound required to produce a 2-fold rightward shift in the concentration-response curve. by Senktide. The functional test on human receptors allows the determination of the concentration of each single compound necessary to reduce by 50% (IC50 values) the mobilization of Ca ++ induced by the NKB agonist. In this test the compounds of the present invention behave as antagonists.

The activity of the compounds of the present invention, as ligands of the NK2 receptor, is determined by their ability to inhibit the binding of the radiolabeled ligands of the receptor

NKA, to human NK2 receptors (Aharony et al, 1992, Neuropeptide, 23, 121-130).

The binding tests used allow the determination of the concentration of the single compound necessary to reduce the specific binding of to the NK2 receptor by 50% in equilibrium conditions (IC50).

The binding tests provide for each compound examined an average value of separate experiments performed in duplicate or in triplicate. The more potent compounds of the present invention show IC5Q values in the range of 1-1000 nM. The NK2-antagonist activity of the compounds of the present invention is determined by their ability to inhibit the mobilization of Ca ++ mediated by human NK2 receptors (Mochizuki et al., 1994, J. Biol. Chem., 269, 9651-9658). The functional test on human receptors allows the determination of the concentration of each single compound necessary to reduce by 50% (IC50 values) the mobilization of Ca ++ induced by the NKA agonist. In this test the compounds of the present invention behave as antagonists.

The therapeutic potential of the compounds of the present invention in the treatment of Conditions can be determined using disease models with rodents.

As indicated above, the compounds of formula (I) are also considered useful as a diagnostic tool. Accordingly, the invention comprises a compound of formula (I) for use as a diagnostic tool to assess the degree to which neurokinin-3 and neurokinin-2 receptor activity (normal, hyperreactivity or hyporeactivity) is involved in a patient's symptoms. . This use includes the use of a compound of formula (I) as an antagonist of said activity, for example including, but not limited to, the turnover of inositulphosphate induced by tachykinin agonists, or the electrophysiological activation of a cell sample obtained from a patient. The comparison of this activity in the presence or absence of a compound of formula (I) will allow to evaluate the degree of involvement of the NK3 and NK2 receptors in the mediation of the agonist effects in this tissue.

The following Descriptions illustrate the preparation of the intermediates, while the Examples illustrate the preparation of the compounds of the invention.

DESCRIPTION 1

10 g (37.98 mol) of 3-methyl-2-phenylquinolin-4-carboxylic acid (CAS [43071-45-0]) was suspended in 1000 ml of 1,2-dichloroethane, 13 were added, 67 g (76.80 mol) of N-bromosuccinimide, 1.0 g (4.13 mol) of dibenzoyl peroxide and the suspension was refluxed for 24 hours. After cooling, the reaction mixture was evaporated under vacuum and taken up in 150 ml of anhydrous THF. 19.63 g (193.99 moles) of TEA and 19.68 g (118.89 moles) of L-proline methylester hydrochloride were added; the mixture was stirred at room temperature for 6 hours and evaporated under vacuum. The crude oil was taken up with a saturated solution and evaporated under vacuum until dry. The solid residue obtained was washed with Et20, acidified with HC16N and the solution evaporated under vacuum until dry. The crude solid was stirred in MeOH, the solution was filtered, concentrated in vacuo, and the resulting oil was purified by gradient chromatography on siliee gel column, 70-230 mesh, using a 90:10 mixture. containing 1% NH4OH (28%) as starting eluent and a mixture of

containing as final eluent. The obtained product was dissolved in acidified with and the precipitate, recovered by filtration, gave 0.56 g of the desired compound as a brownish solid.

EXAMPLE 1

1.0 g (2.56 moles) of the 3 - [{S) -2- (methoxycarbonyl) pyrrolidinl-yl] methyl-2-phenylquinolin-4-carboxylic acid were dissolved, in a nitrogen atmosphere, in 30 ml of a mixture of THF / CH3CN 80:20. The solution was cooled to 0 "C and 0.38 g (2.81 mol) of HOBT, 0.36 g (3.58 mol) of TEA and 0.42 g (3.06 mol) of (S) -aethylbenzylamine. The solution was stirred 5 minutes and 0.58 g (2.81 moles) of DCC dissolved in 18 ml of was dropped. The mixture was stirred 2 hours at 0 ° C, then the temperature was was allowed to rise to room temperature and the reaction stirred for 3 hours. The precipitated dicyclohexylurea was filtered and the filtrate was evaporated under vacuum to dryness. The residue was dissolved in and washed with INN NaOH. The organic phase was dried-up

and evaporated under vacuum to dryness. The crude product was

purified by gradient chromatography on silica gel column, 70-230 mesh, using a mixture of hexane / AcOEt 90:10 as starting eluent and a mixture of hexane / AcOEt 70/30 containing 1% (28%) as eluent the final. The obtained product was dissolved in and acidified with 2 the formed precipitate was recovered by filtration and gave 0.29 g of the desired compound.

EXAMPLE 2

1.6 g (3.2 mol) of (S) -N- (a-ethylbenzyl) -3 - [(S) -2- (methoxycarbonyl) pyrrolidin-1-yl] methyl-2-phenylquinolin-4-carboxamide (compound of Example 1) were dissolved, in a nitrogen atmosphere, in 40 ml of t-BuOH; 0.6 g (15.86 moles) of the solution were added, it was heated to 80 ° C, 5 ml of MeOH was dropped and the mixture was refluxed for 6 hours. After cooling, the reaction was quenched with 10 ml of water and 10 ml of 6N NaOH. The solution was evaporated to dryness under vacuum and the residue was taken up in AcOEt. 1.5 g of silica gel was added and the suspension was stirred for 1 hour, filtered and evaporated in vacuo to dryness to yield 0.4 g of the desired product.

EXAMPLE 3

The silica gel filtered in the last step of Example 2 was suspended in MeOH and stirred for 2 hours. The mixture was filtered and evaporated in vacuo to dryness to yield 0.24 g of the desired product.

Following the same procedure described in Example 1 and starting from the appropriate amino acids of formula II (prepared as indicated in Description 1), and from the secondary amines of formula PhCH (HH2) R /, the compounds of Examples 4, 6-11 were prepared. , 15, 16, 19, 20 and 26.

The compound of Example 5 was prepared as described in the procedure of Example 2.

DESCRIPTION 2

4-Hydroxyphenylbutanone

11.2 g (130.01 moles) dijf-butyrolactone were dissolved, in a nitrogen atmosphere, in 50 ml of anhydrous THF. The solution was cooled to -78 ° C and 21.7 ml (43.4 moles) of phenyllithium 2 M in diethyl ether were added by dropping and maintaining the temperature between -60 and -70'C. The temperature was allowed to rise to room temperature and the solution was stirred for 1 hour. The reaction was quenched with 30 ml of 10% NH4CI, diluted with water, extracted with Et20 and evaporated in vacuo to dryness. The crude product was dissolved in 100 ml of EtOH, 10 g of KOB beads were added and the mixture was stirred at room temperature overnight. The solvent was evaporated under vacuum and the residue, dissolved in water, was extracted with Et20. The organic phase was dried with Na2SO4, evaporated under vacuum and purified by flash chromatography on a silica gel column, 230-400 mesh, using a mixture of hexane / AcOEt 7: 3 as eluent. 8 g of the desired product were obtained.

DESCRIPTION 3

5.0 g (30.40 mol) of 4-hydroxy-phenylbutanone and 4 g (39.51 mol) of TEA were diluted, in a nitrogen atmosphere, in 80 ml of

anhydrous. The solution was cooled to 0 ° C and 4.12 g (35.93 moles) of methanesulfonyl chloride were added by dropping and maintaining the temperature between 0 and 5 ° C. The temperature was allowed to rise to room temperature and stirring continued for 1 hour. The solution was then washed with 40 ml of cold water and the organic phase was dried over Na2SO4 and concentrated under vacuum. The crude oil obtained was dissolved in 25 ml of DMF, 9.82 g (60.80 moles) of phenylpiperazine were added and the reaction was stirred for 4 hours at room temperature. The reaction mixture was evaporated under vacuum, the residue treated with AcOEt and filtered. The organic phase was washed with H2O, evaporated in vacuo to dryness and the residue purified by flash chromatography on a silica gel column, 230-400 mesh, eluting with to give 2.1 g of the desired compound.

DESCRIPTION 4

1.15 g (7.80 mol) of isatin was suspended in 20 ml of absolute EtOH and 1.4 g (21.45 mol) of 85% KOH was added. After stirring for 30 minutes at room temperature, 2 g (6.50 moles) of 4- (4-phenyl-4-keto) butyl-1-phenylpiperazine were added and the mixture was refluxed for 2 days. After cooling to room temperature, the solution was acidified with HC16 N to pH = 7 and extracted with AcOEt. The organic phase was dried-up

and evaporated under vacuum to dryness. The residue was

purified by flash chromatography on a silica gel column, 230-400 mesh, eluting with AcOEt / MeOH 80:20 containing 2% of. The crude solid was triturated in hot AcOEt and 1.24 g of the desired compound was obtained as a white powder.

The compounds of Examples 12-14, 21-25, 27, 29 and 30 were prepared following the same procedure described in Example 1 and starting from the appropriate amino acids of formula II prepared according to Description 4.

EXAMPLE 17

bossairanide

0.1 g (0.192 mol) of (S) -N- (a-ethylbenzyl) -3 - [(1,4-dioxo-8-azaspiro [4.5] decan-8-yl) methyl] -2-phenylquinolin- 4-carboxamide (compound of Example 9) were dissolved in 20 ml of HC16 N and stirred at room temperature for 24 hours. The solution was basified with 6 N NaOH and extracted with AcOEt. The organic phase was dried on

and evaporated in vacuo to dryness to yield 0.040 g of

desired product.

EXAMPLE 18

carboxamide

0.38 g (0.79 moles) of (S) -N- (a-ethylbenzyl) -3 - [(4-piperidon-lil) methyl] -2-phenylquinolin-4-carboxamide (compound of Example 17) were dissolved in 15 ml of MeOH and cooled to 5 "C. 0.095 g of (2.5 mol) was added and stirred at 5'C for 3 hours. The solution was quenched with 6 N HCl and MeOH is concentrated under vacuum. The crude product obtained was basylated with 6 N NaOH and extracted with The organic phase was dried on and evaporated under vacuum to dryness. The crude product was purified by chromatography on a silica gel column, 70-230 mesh, using a 60:40 AcOEt / hexane mixture containing 1% NH4OH (28%) as eluent The collected fractions yielded 0.32 g of the desired compound.

EXAMPLE 28

(S) -N- (α-Ethylbenzyl) -3 - [(piperazin-1-yl) methyl] -2-phenylquinolin-4-carboxamide

1.75 g (3.2 mol) of (S) -N- (a-ethylbenzyl) -3 - [(4-benzylpiperazin-lil) methyl3-2-phenylquinolin-4-carboxamide (compound of Example 26) are having been dissolved in 50 ml of EtOH, 0.5 g Pd (0H) 2 and 0.8 g (12.6 moles) of ammonium formate are added. The mixture is refluxed and 0.12 ml (3.2 moles) of formic acid are added. After 1 hour under reflux the mixture is cooled, the catalyst is filtered, the solvent is evaporated, taken up in H20O and extracted with AcOEt. The organic phase was dried over Na2SO4 and evaporated under vacuum to dryness. The crude product was purified by flash gradient chromatography on a silica gel column, 230-400 mesh, using a 90:10 AcOEt / MeOH mixture containing 1% N (28%) as starting eluent and a 80:20 AcOEt / MeOH mixture containing 1% NH4OH (28%) as final eluent. The obtained oil was triturated in ethyl ether to yield 1.1 g of the desired product.

Claims (10)

CLAIMS 1. Compound, or its solvate or salt, of formula (I) (I) in which Ar is a possibly substituted aryl or group cycloalkadienyl, or a group or an aromatic heterocyclic group with a single or fused ring, optionally substituted; R. is phenyl or optionally substituted phenyl, an optionally substituted five-membered heteroaromatic ring, comprising up to four heteroatoms selected from 0 to N, hydroxy amino phthalimido, amino or mono- and ^ g R2 represents a residue in which n is an integer in the range from 1 to 9, and Y2 are independently selected from hydrogen; ^ g jg substituted with hydroxy, alkylamino or bis; aryl or arii together with the nitrogen atom to which they are bonded represent an N-bonded, optionally substituted, single or fused ring heterocyclic group; R3 is branched or linear, cycloalkylalkyl, optionally substituted aryl, or an aromatic heterocyclic group with single or fused ring, optionally substituted; And represents hydrogen or C 2. Compounds according to claim 1, wherein Ar represents optionally substituted phenyl. Compound according to claim 1, wherein R represents alkyl, for example ethyl or iso-propyl. 4. Compound according to claim 1, wherein R? Represents hydrogen. Compound according to claim 1, wherein NY ^ Y2 represents an N-linked, optionally substituted, single or fused ring heterocyclic group. Compound according to claim 5, wherein the N-bonded single or fused ring heterocyclic groups comprise groups in which any single or fused ring is saturated or unsaturated and comprises 5 or 6 ring atoms, said ring atoms possibly comprising 1 or 2 additional heteroatoms selected from 0 or N, and in which one or two ring atoms are optionally replaced with one or two oxo groups or with one or two of hydroxy, carboxy, alkyl, aryl, arylalkyl, or a single or fused aromatic heterocyclic group, or the substituents on adjacent ring atoms form a carbocyclic ring; said aryl or aromatic heterocyclic groups being optionally substituted with one or two alkoxy, hydroxy, halogen or halogenalkyl groups. 7. Process for the preparation of a compound of formula (I), or of a salt and / or solvate thereof, which comprises the reaction of a compound of formula (II) or of an activated derivative: (II) in which they are respectively as defined in relation to formula (I) or a convertible group respectively-in with a compound of formula (III): (III) wherein respectively as defined by formula (I) or a group or atom convertible respectively into and Ar; so as to form a compound of formula (Ib) (ib) in which they are as defined above, and subsequently carrying out one or more of the following optional stages: (i) converting any one of into respectively, as necessary, so as to obtaining a compound of formula (I); (ii) conversion of compound of formula (I) into another compound of formula (I); And (iii) preparation of a salt of the compound of formula (I) and / or of a solvate thereof. 8. Pharmaceutical composition comprising a compound of formula (I), or a pharmaceutically acceptable salt or solvate thereof, and a pharmaceutically acceptable carrier. 9. Compound of formula (I), or a pharmaceutically acceptable salt or solvate thereof, for use as an active therapeutic substance. 10. Use of a compound of formula (I), or a pharmaceutically acceptable salt or solvate thereof, in the manufacture of a medicament for the treatment of Primary and Secondary Conditions.