IT201900013488A1 - BIOACTIVE AND VEGETABLE ANTIOXIDANT DRINK FOR THE IMPROVEMENT OF THE INTEGUMENTARY SYSTEM AND THE FORMATION OF COLLAGEN - Google Patents

Description

Description of the industrial invention entitled:

"Bioactive and plant-based antioxidant drink for the improvement of the integumentary system and the formation of collagen"

TEXT OF THE DESCRIPTION

Field of the invention

The present description relates to compositions particularly suitable for counteracting the oxidative damage of the integumentary system.

Background of the invention

Oxidative stress is defined as a disturbance in the balance between the production of reactive oxygen species (ROS) and antioxidant defenses. ROS are molecules containing reactive oxygen molecules; examples of ROS include free radicals, oxygen ions and peroxides. ROS are formed as a natural process of normal oxygen metabolism and have important roles in cell signaling and homeostasis. ROS can also act by guiding several molecular pathways that play important roles in distinct pathological conditions such as cancer, heart disease and diabetes. The overproduction of peroxides and free radicals leads to damage to cell components, including proteins, lipids and DNA. Additionally, some ROS can act as cellular messengers in reduction / oxidation (redox) signaling between cells. Therefore, oxidative stress can cause alterations in normal cellular signaling mechanisms. Therefore, the use of oxygen by the cells of aerobic organisms generates potentially harmful reactive oxygen metabolites (ROM). The extent of oxidative damage can increase with the age of an organism and may be the heaviest factor capable of leading the cell to a state of senescence. The main point that emerges from the research studies is that molecular oxidative damage during aging is ubiquitous, significant and significantly increases with age. The main causes of an age-associated increase in the amount of oxidative stress can be linked to an increase in the rate of ROM generation, a decrease in antioxidative defenses, a decrease in the efficiency of repair or removal of damaged molecules. The production of hydrogen peroxide by the mitochondria is also a major cause of oxidative stress and in mammals increases with age in various different organs.

The skin, along with hair and nails, is the main organ of the integumentary system and represents the first and main barrier to the external environment.

In the skin, free radical damage can cause deterioration of the supporting connective tissue, resulting in a reduction in elasticity and resilience. Furthermore, oxidative stress can impair the function of the hair follicle, causing androgenetic alopecia (baldness) in men and women.

Exposure of the skin to ultraviolet radiation from the sun initiates photochemical reactions that lead to the formation of ROS and when their production goes out of control, oxidative stress occurs. When this happens, ROS (together with enzymes released by granules inside white blood cells) damage or even kill cells, damage DNA and attack enzymes and other compounds.

The skin has defense mechanisms that interact with toxic agents and counter their damaging effect (such as non-enzymatic and enzymatic molecules that act as powerful antioxidants). The cell rigidly regulates antioxidant responses through key pathways such as Nrf2 (nuclear factor (erythroid-derived 2) -like 2), which is known as the Principal Regulator of antioxidant responses. Loss of function or the reduced ability of Nrf2 to respond to ROS can lead to cell damage, inflammation and disease. When these defenses are overwhelmed, this can lead to increased ROS production in situ and the consequent development of a dermatological disease. It is generally agreed that there is a correlation between aging and the accumulation of oxidatively damaged proteins, lipids and nucleic acids.

One approach to prevent or treat these ROS mediated disorders is based on the administration of compositions comprising antioxidants, i.e. molecules capable of stabilizing or deactivating free radicals before they cause damage in healthy cells.

The antioxidant compositions may comprise antioxidant compounds, such as amino acids, peptides, proteins and lipids. This includes a wide variety of amino acids with thiol groups such as glutathione, peptides such as albumin and globulin from legumes or lipids such as carotenoids and tocopherols. Collagen supplements to improve skin condition are typically derived from animal proteins, such as cattle, fish or pigs. However, the number of people who advocate veganism or vegetarianism as a specific lifestyle philosophy has grown dramatically in recent years. It is therefore becoming increasingly important to develop new compositions, from non-animal sources, which exert similar or even increased beneficial antioxidant effects, which may be suitable for satisfying the nutritional and well-being needs of these consumers.

Summary of the invention

The purpose of the present description is to provide plant-based and antioxidant compositions capable of slowing down the natural aging process by counteracting the formation of free radicals in the body, increasing the production of collagen and / or elastin and reducing the degradation of collagen and / or elastin through synergistic effects exerted by the active ingredients.

According to this description, the above purpose is achieved thanks to the object specifically referred to in the following claims, which are intended as an integral part of this description.

The present description provides a composition comprising emblica extract (Emblica officinalis or Amla), pomegranate extract (Punica granatum), horsetail extract (Equisetum arvense).

In one or more embodiments, the composition further comprises at least one amino acid selected from the group consisting of glycine, lysine, proline.

In one or more embodiments, the composition comprises at least one further component selected from the group consisting of rice peptide hydrolyzate (Oryza sativa), borage oil, black pepper extract.

In one or more embodiments, the composition described herein may further comprise a component selected from the group consisting of gotu kola extract, copper, vitamin E.

In one or more embodiments, the composition described herein may also include minerals, additives and flavoring substances.

In one or more embodiments, the composition is free of animal proteins.

In one or more embodiments, the composition is a composition in the form of a beverage.

The description also provides the use of the composition to reduce oxidative damage to the integumentary system and to promote the formation of collagen and elastin.

In one or more embodiments, a method is also provided for reducing oxidative damage to a subject's integumentary system and promoting the formation of collagen and elastin, the method comprising the steps of administering the composition to the subject, wherein the composition reduces oxidative damage and promotes the formation of collagen and elastin.

Brief description of the drawing

The invention will be described, purely by way of example, with reference to the attached figure 1 which shows a diagram of the production process of an embodiment of a composition described in this description.

Detailed description of the invention

In the following description, numerous specific details are provided to allow a thorough understanding of the embodiments. Embodiments can be practiced without one or more of the specific details, or with other processes, components, materials, etc. In other cases, well-known structures, materials or operations are not shown or described in detail to avoid confusing aspects of the embodiments.

Reference throughout this description to "one embodiment only" or "one embodiment" means that a / a particular aspect, structure or feature described / described in relation to the embodiment is included / included in at least one embodiment of realization. Therefore, the forms of the expressions "in one embodiment" or "in one embodiment" at various points throughout the present disclosure do not necessarily all refer to the same embodiment. Furthermore, the particular aspects, structures or features can be combined / combined in any suitable way in one or more embodiments.

The titles provided here are for convenience only and do not interpret the scope or meaning of the embodiments.

The purpose of the present description is to provide antioxidant and plant-based compositions capable of slowing down the natural aging process by counteracting the formation of free radicals in the body. These free radical species would normally cause the depletion of cellular levels of endogenous antioxidants such as Vitamin C, leading to a consequent reduction in the production of collagen and / or elastin. The carefully chosen antioxidant compositions presented here are able to enhance the production of collagen and elastin, reduce the degradation of collagen and / or elastin through the specific effects exerted by the active ingredients.

In one or more embodiments, the present disclosure provides a composition comprising emblica extract, pomegranate extract, horsetail extract.

The composition has a high antioxidant capacity; is able to reduce oxidative damage and improve the health of the integumentary system. Furthermore, thanks to the nature of the components contained within it, the composition has been adapted to meet the nutritional and well-being needs of those consumers who have a vegan and / or vegetarian diet. Multiple clinical studies show that a vegan diet - i.e. avoiding the consumption of all animal products such as meat, fish, dairy products and eggs - can have a positive impact on health, also due to the high fiber content and the reduction in cholesterol intake. On the other hand, a vegan diet can be associated with a number of nutritional deficiencies.

The composition described here is able to meet important and specific nutritional needs related to adherence to a strict vegan diet and, at the same time, exercise an improvement in the condition of the skin by increasing hydration and elasticity, decreasing the depth and the total surface area of wrinkles, keeping skin, hair and nails healthy, increasing the production of collagen and / or elastin and reducing their degradation.

The compositions described herein can be produced in either liquid or solid form, i.e. by mixing the ingredients in solid form or by lyophilizing the liquid preparation.

The compositions can be administered as food supplements. The compositions described herein can be in the form of a beverage.

The composition can be administered in an amount of between 10 ml and 100 ml, preferably in an amount of between 20 ml and 80 ml, more preferably in an amount of between 30 ml and 50 ml of daily dosage for a minimum of 30 days, preferably for 60 days, more preferably for 90 days.

The composition comprises emblica extract (Emblica Officinalis) in an amount comprised between 0.5 mg / 50 ml and 250 mg / 50 ml, preferably in an amount comprised between 5 mg / 50 ml and 150 mg / 50 ml, more preferably in a quantity between 10 mg / 50 ml and 100 mg / 50 ml.

The emblica, also called Amla, Phyllanthus emblica, Emblica officinalis, is a deciduous tree with edible fruits with a high content of Vitamin C. The fruit is similar in appearance to the common gooseberry (Ribes spp., A type of currant) , which is botanically unrelated to Amla. However, due to the similar appearance of the fruit clusters, Amla is usually called "Indian gooseberry".

The emblica extract for use in the composition described here is a 100% natural extract of the Phyllanthus emblica fruit; the extraction is carried out in water. Emblica extract is commercially available from a variety of different sources. For example, emblica extract can be obtained from Han Sient Trading Co., Ltd (New Taipei City, Taiwan), Nexira (Rouen, France), Carob, Inc (Milford, Connecticut), Mountain Rose Herbs (Eugene, Oregon ), Pfanneschmidt (Hamburg, Germany), Gonmisol (Barcelona, Spain). The emblica extract can also be isolated or purified using standard isolation and purification techniques.

The emblica extract useful in the composition described here can be a dry extract in powder form. The powder can be dissolved in hot water (85 ° C ± 5 ° C) in a mixing tank together with pomegranate extract and horsetail extract, as described below, in the example of the composition production process.

The composition comprises pomegranate extract in an amount of between 0.5 mg / 50 ml and 250 mg / 50 ml, preferably in an amount of between 5 mg / 50 ml and 150 mg / 50 ml, more preferably in an amount of between 10 and 100 mg / 50 ml.

Pomegranate is a powerful source of polyphenols, in particular anthocyanins, with a wide range of health benefits, including the inhibition of oxidative stress.

The pomegranate extract used in the composition described here is an extract from the fruit (pericarp) of the Punica granatum L plant. The extraction is an extraction in ethanol / hot water. Commercially available extraction liquid powder can be used. Pomegranate extract is commercially available from a variety of different sources. For example, pomegranate extracts can be obtained from JIAHERB Phytochem Co. (Xi'an, China), Ltd, Nexira (Rouen, France), Carob, Inc (Milford, Connecticut), Mountain Rose Herbs (Eugene, Oregon), Pfanneschmidt (Hamburg, Germany), Gonmisol (Barcelona, Spain). The extract can also be isolated or purified from plants containing these extracts using standard isolation and purification techniques. The useful extract in the compositions described here can be a dry extract in powder form. The powder can be dissolved in hot water (85 ° C ± 5 ° C) in the mixing tank together with the emblica extract and the horsetail extract, as described below, in the example of the composition production process.

Horsetail extract may be present in the composition in an amount of between 0.5 mg / 50 ml and 100 mg / 50 ml, preferably in an amount of between 2.5 mg / 50 ml and 50 mg / 50 ml, plus preferably in an amount ranging from 5 mg / 50 ml to 20 mg / 50 ml.

The horsetail, or equisetum, is a group of herbaceous plants of the Equisetaceae family. One of the elements of the Equisetum group is Equisetum arvense, which is commonly called horsetail. Horsetail extract has an anti-inflammatory and antioxidant effect for the protection of cells from oxidative stress; it is also capable of having beneficial effects on the health of the skin, hair and bones.

The horsetail extract used in the composition described here is an extract of the entire Equisetum arvense plant. The extraction is a hot extraction in ethanol / water (30:70). Commercially available extraction liquid powder can be used. Horsetail extract is commercially available from a variety of different sources. For example, it can be obtained from Material World Industrial Co. (New Taipei City, Taiwan), Ltd, Carruba Inc (Milford, Conneticut), Naturex (South Hackensack, New Jersey), Aunatura Industries Inc (Chino, California, USA), Parchem (New Rochelle, New York), Nexira (Rouen, France). The extract can also be isolated or purified using standard isolation and purification techniques. The extract useful in the composition described here can be a dry extract in powder form. The powder can be dissolved in hot water (85 ° C ± 5 ° C) in the mixing tank together with the emblica extract and the pomegranate extract, as described below, in the example of the composition production process.

In one or more embodiments, the composition may comprise emblica extract: pomegranate extract: horsetail extract in a weight ratio of 12: 6: 1, preferably 10: 6: 1, more preferably 8: 5: 1.

Due to the effects exerted by its main ingredients, namely emblica extract, pomegranate extract, horsetail extract, the composition described here has a high antioxidant capacity and is able to reduce oxidative damage to the integumentary system and at the same time to stimulate the production of collagen and / or elastin while reducing their degradation.

In particular, by counteracting the formation of free radicals, it improves the condition of the skin by increasing its elasticity and firmness, decreasing dryness, depth and the total surface of wrinkles. It also keeps skin, hair and nails healthy.

In one or more embodiments, the composition may further comprise at least one amino acid selected from the group consisting of glycine, lysine, proline.

The inventors of the present application have discovered that by adding at least one amino acid selected from the group consisting of glycine, lysine, proline, a surprising increase in the effect exerted by the composition is obtained, in terms of increasing the production of collagen and / or elastin. , reduction of collagen and / or elastin degradation, increase in skin elasticity, reduction of wrinkles, maintenance of healthy skin, hair and nails.

The amino acids glycine and proline are the main components of collagen, which is the main component of the skin, especially in the compartment of the extracellular matrix at the level of the dermis, and together with elastin and hyaluronic acid it plays a key role in providing integrity and elasticity . Furthermore, glycine has been shown to promote the synthesis of glutathione, which exerts antioxidant effects. Lysine is an essential amino acid that supports collagen synthesis.

Glycine can be comprised in the composition in an amount comprised between 1 mg / 50 ml and 250 mg / 50 ml, preferably between 10 mg / 50 ml and 150 mg / 50 ml.

Lysine can be comprised in the composition in an amount comprised between 1 mg / 50 ml and 800 mg / 50 ml, preferably between 10 mg / 50 ml and 700 mg / 50 ml, more preferably between 300 mg / 50 ml and 600 mg / ml.

The proline can be comprised in the composition in an amount comprised between 1 mg / 50 ml and 300 mg / 50 ml, preferably between 10 mg / 50 ml and 200 mg / 50 ml.

Further advantageous effects are observed when the composition comprises at least one further component selected from the group consisting of hydrolyzate of Oryza sativa (rice peptides), borage seed oil, black pepper extract.

Oryza sativa is a non-animal source of protein. 100% hydrolyzed rice peptides are extracted from the cracked rice grain. It is an enzymatic hydrolyzate from rice grain proteins that can be obtained in the form of granulated or fine powder. Rice peptide hydrolyzate is commercially available from a variety of different sources. For example, rice peptide hydrolyzate can be obtained from Nexira (Rouen, France), Shafi Gluco Chem (Kerachi, Pakistan), ET-Chem (Suzhou, China), Beneo GmbH (Mannheim, Germany), Ingredients UK ( Berkshire, UK), Oryza Oil and Fat Chemical Co. Ltd. (Aichi, Japan) The rice peptide hydrolyzate useful in the compositions described herein comprises at least 70% peptides. It is a powder that can be dissolved in hot water (60 ° C ± 5 ° C) in a mixing tank, as described below, in the example of the composition production process.

The rice peptide hydrolyzate Oryza sativa can be present in the composition in an amount of between 0.5 mg / 50 ml and 100 mg / 50 ml, preferably in an amount of between 1 mg / 50 ml and 50 mg / 50 ml , more preferably in an amount ranging from 3 mg / 50 ml to 10 mg / 50 ml.

Borage oil can be present in an amount of between 0.5 mg / 50 ml and 250 mg / 50 ml, preferably in an amount of between 2 mg / 50 ml and 200 mg / 50 ml, more preferably in an amount between 5 mg / 50 ml and 150 mg / 50 ml.

It is known that black pepper extract increases the bioavailability of various substances by increasing their gastrointestinal absorption. The extract is an extract from the fruit of the Piper nigrum plant. The extract useful in the composition described here (commercially available for example from Sabinsa Japan Corporation, East Windsor NJ, USA) is a dry extract in the form of a powder. The extract can be present in an amount of between 0.2 mg / 50 ml and 3.5 mg / 50 ml, preferably between 0.5 mg / 50 ml and 2.5 mg / 50 ml, more preferably in an amount between 1 mg / 50 ml and 2 mg / 50 ml.

By adding at least one further component chosen from the group consisting of rice peptide hydrolyzate, borage oil, black pepper extract, the composition described here surprisingly shows an enhancing effect on the smoothness and hydration of the skin, in the production of collagen and elastin and in the inhibition of their degradation.

In particular, the hydrolyzed rice peptides Oriza sativa stimulates the production of collagen and / or elastin, maintains skin elasticity, promotes cell regeneration and protects the skin from free radical damage.

Borage oil contains gamma-linolenic acid (GLA), rich in omega-6 fatty acids, which are able to improve the smoothness and hydration of the skin.

Advantageous results in terms of production of collagen fibrils in fibroblasts, secretion in the extracellular matrix and assembly to provide elasticity and compactness and overall improvement of skin hydration can be obtained by adding in the composition at least one further ingredient chosen from the group consisting of Centella extract. asiatica, copper, vitamin E.

Centella asiatica extract is a rich source of natural bioactive substances, amino acids, beta-carotene, fatty acids, phytochemicals and exerts antioxidant effects that promote the protection of cells from oxidative stress.

Centella asiatica extract is an extract from the leaves of the plant. The extraction is a hot ethanol extraction. Commercially available extraction liquid powder can be used. Centella asiatica extract is commercially available from a variety of different sources. For example, gotu kola extract can be obtained from Biomed Herbal Research Co., Ltd (Taichung City 404, Taiwan), Naturex (South Hackensack, New Jersey), Mountain rose Herbs (Eugene, Oregon), Pfanneschmidt (Hamburg, Germany), Denk ingredients (Munich, Germany). The extract can also be isolated or purified using standard isolation and purification techniques. The useful extract in the compositions described here can be a dry extract in powder form. The powder can be dissolved in hot water (85 ° C ± 5 ° C) in a mixing tank, optionally together with citrus bioflavonoids and blueberry extract, as described below, in the example of the composition production process.

Centella asiatica extract can be present in the composition in an amount ranging from 0.5 mg / 50 ml to 25 mg / 50 ml, preferably in an amount ranging from 1 mg / 50 ml to 15 mg / 50 ml, more preferably in a quantity between 1.5 mg / 50 ml and 10 mg / 50 ml.

Copper contributes to normal skin and hair pigmentation and protects cell constituents from oxidative damage. It also plays a role in maintaining healthy connective tissue by contributing to normal energy metabolism, normal nervous system functioning, normal iron transport in the body, normal immune system function, and protecting cells from oxidative stress. Copper may also be present in the composition in the form of salts, for example in the form of copper gluconate.

Copper can be present in an amount between 0 and 1000 μg / 50 ml, preferably in an amount between 50 μg / 50 ml and 500 μg / 50 ml, more preferably in an amount between 100 μg / 50 ml and 250 μg / 50 ml.

Vitamin E can be present in an amount ranging from 0 to 15 mg / 50 ml, preferably in an amount ranging from 1 to 10 mg / 50 ml, more preferably in an amount ranging from 2 to 6 mg / 50 ml.

The present Inventors have discovered that by adding to the composition at least one further component chosen from the group consisting of Centella asiatica extract, copper, Vitamin E, a further strengthening effect was obtained in terms of skin hydration, skin elasticity, collagen production and / or elastin and their degradation, antioxidant activity and bioavailability of various substances.

In addition to vitamin E, other vitamins may be present in the composition, for example vitamins selected from the group consisting of vitamin B2, vitamin B5, vitamin B6, vitamin B12, vitamin C, biotin, folic acid and other water-soluble vitamins.

Bilberry extract and / or citrus bioflavonoids which are a rich source of antioxidants can also be present in the composition, in an amount of each of them between 0 mg / 50 ml and 100 mg / 50 ml, preferably between 0.5 mg / 50ml and 50mg / 50ml.

Bilberry extracts (Vaccinium angustifolium Aiton) are commercially available from a variety of different sources. For example, blueberry extract can be obtained from Diana Food SAS (Rennes, France), Nexira (Rouen, France), Biomed Herbal Research Co., Ltd. (Taichung City, Taiwan), Carob, Inc (Milford, Connecticut ), Mountain Rose Herbs (Eugene, Oregon), Pfanneschmidt (Hamburg, Germany), Gonmisol (Barcelona, Spain). The extract is a dry extract in a fine purple-red powder, obtained from the dehydrated fruit through an extraction in water. Blueberry extracts can be dissolved in hot water (85 ° C ± 5 ° C) in a mixing tank optionally together with citrus bioflavonoids and gotu kola extract, as described below, in the composition manufacturing process example .

In one or more embodiments, the composition can also comprise Tremella fuciformis extract (or snow fungus), which is rich in polysaccharides and which has proved useful for skin hydration; its soluble fiber is also good for intestinal health. Tremella fuciformis is also known as a "source of vegetable collagen".

Tremella fuciformis extract can be present in the composition, in an amount between 0 mg / 50 ml and 2000 mg / 50 ml, preferably between 500 mg / 50 ml and 1500 mg / ml. The extract is obtained by extracting the fruiting body in water. A commercially available extraction liquid can be used. Tremella fuciformis extracts are commercially available from a variety of different sources. For example, the extract can be obtained from Nutra Green Bio (Nutralion, Inc. based in Walnut, California), Zhejiang Biosan Biotech Co, Ltd (Hangzhou, China), Pharchem (New Rochelle, New York, USA), Bristol Botanicals Ltd (Bristol, UK). The extract can also be isolated or purified using standard isolation and purification techniques. The extract of Tremella fuciformis (snow fungus) useful in the composition described here can be a liquid extract (extraction in reverse osmosis water) and must not be dissolved before adding to the composition.

The composition described here is free from animal proteins.

The composition described here may also include minerals, additives and flavoring substances.

Minerals can be chosen from zinc, zinc gluconate, magnesium sulfate, manganese gluconate, manganese gluconate, chromium picolinate, ferrous gluconate.

Furthermore, the composition may also include folic acid. The additives can preferably be selected from pectin and citric acid.

The flavoring substances can be chosen from cherry flavor, blueberry flavor and pomegranate flavor.

Esters, higher aliphatic alcohols, ketones, aromatic aldehydes, aromatic alcohols, propylene glycol, isoamyl alcohol, maltol, vanillin and Bucco essential oil can be used to formulate the aromatic base.

Table 1 reports the content range of the ingredients included in the composition described here.

Table 1

A non-limiting example of a liquid composition object of the present description is shown in Table 2.

Table 2

A non-limiting example of a production process of a composition in the form of a beverage object of the present description will be given below.

Example 1.

A liquid beverage composition comprising the ingredients shown in Table 3 below was prepared as described below and as shown in Figure 1.

Table 3

Ingredients Content 50 ml

Water at a temperature of 85 ° C ± 5 ° C is placed in a first mixing tank. Horsetail extract (Material World Industrial Co., Ltd), emblica extract (AMLA extract - Emblica officinalis; Han-Sient Trading Co., Ltd), citrus bioflavonoids (Espertina, Ciratina, Cirmtina; Roterm Trading Co., Ltd) ), gotu kola leaf extract (Hydrocotyle asiatica extract; Biomed Herbal Research Co., Ltd), pomegranate extract (JIAHERB Phytochem Co., Ltd), blueberry extract (Diana Food SAS) are placed in the first mixing tank to be dissolved and a mixing step is performed for a period of 10 minutes. Sodium hyaluronate, black pepper extract (Bioperine <®>; Sabinsa Japan Corporation), glycine, L-proline, L-lysine monohydrochloride are then added to the first in order mixing tank. The ingredients are mixed over a period of 10 minutes to obtain a solution. The solution is transferred to a charge tank. Water (60 ° C ± 5 ° C) is then placed in a second mixing tank. Vitamin B2, Vitamin B5, Vitamin B6, Vitamin B12, Copper Gluconate, Zinc Gluconate, Biotin, Selenium Yeast, Magnesium Sulphate, Manganese Gluconate, Chromium Picolinate, Folic Acid, Rice Peptide Hydrolyzate (Oryza sativa) (ORYZA PROTEIN <TM> - P70 (Food); Oryza oil & Fat Chemical Co., Ltd), ferrous gluconate are put into the second mixing tank in order. A mixing phase follows for 10 minutes, then the solution is transferred to the charge tank. Water (25 ° C ± 5 ° C) is then placed in a third mixing tank in which borage seed oil (Lipoid) and d-α-tocopherol (4% Vitamin E emulsion) are added. A mixing step is performed for 10 minutes. Liquid extract of snow fungus (Tremella fuciformis) (TCI Co., Ltd.) is added to the mixing tank and a further mixing step is performed for 10 minutes. The solution is then transferred to the charge tank. The temperature of the final solution is controlled; the temperature should be 45 ° C or less. Vitamin C is placed in the charge tank and a mixing step is performed for 5 minutes. Before filtration and sterilization, the bottles go through a quality control of the semi-finished product by testing flavor, pH and brix. The final mixture was filtered to ensure that the solution was clear and free of particles. Subsequently, the solution is sterilized through a pasteurization system at 105 ° C ± 2 ° C for 30 seconds. Once sterilized, the solution is introduced into the bottle at a high temperature (80 ° C-85 ° C) and the bottle is capped. The bottles can then be stored under appropriate conditions, prior to distribution.

The compositions of the present description, being substantially free of products of animal origin, can be advantageously taken by people who follow a vegetarian or vegan diet.

The composition is able to reduce oxidative damage to the integumentary system and to promote the production of collagen and / or elastin while reducing their degradation. In particular, taking the composition reduced skin dryness, improved skin firmness and elasticity and exerted an anti-aging effect by decreasing the depth and total surface area of wrinkles. An increase in collagen and / or elastin levels was observed and their degradation was reduced.

The realization of the composition is not limited to these examples but may have variants that do not exceed the limits of the claims reported below.

Of course, while the principle of the invention remains the same, the manufacturing details and embodiments can vary widely with respect to what has been described and illustrated purely by way of example, without departing from the scope of the present invention.

Claims (11)

CLAIMS 1. Composition comprising emblica extract, pomegranate extract, horsetail extract. 2. Composition according to claim 1, wherein the emblica extract is present in an amount comprised between 0.5 mg / 50 ml and 250 mg / 50 ml, preferably in an amount comprised between 5 mg / 50 ml and 150 mg / 50 ml, more preferably between 10 mg / 50 ml and 100 mg / 50 ml. Composition according to claim 1 or claim 2, wherein the pomegranate extract is present in an amount comprised between 0.5 mg / 50 ml and 250 mg / 50 ml, preferably in an amount comprised between 5 mg / 50 ml and 150 mg / 50 ml, more preferably between 10 mg / 50 ml and 100 mg / 50 ml. Composition according to any one of the preceding claims, wherein the horsetail extract is present in an amount comprised between 0.5 mg / 50 ml and 100 mg / 50 ml, preferably in an amount comprised between 2.5 mg / 50 ml and 50 mg / 50 ml, more preferably between 5 mg / 50 ml and 20 mg / 50 ml. 5. Composition according to any one of the preceding claims, wherein the emblica extract, the pomegranate extract, the horsetail extract are present in the composition in a weight ratio of emblica extract: pomegranate extract: equal horsetail extract at 12: 6: 1, preferably 10: 6: 1, more preferably 8: 5: 1. 6. Composition according to any one of the preceding claims, comprising at least one amino acid selected from the group consisting of glycine, lysine, proline. 7. Composition according to any one of the preceding claims, further comprising at least one further ingredient selected from the group consisting of hydrolyzed rice peptides, borage oil, black pepper extract. 8. Composition according to any one of the preceding claims, further comprising at least one ingredient selected from the group consisting of copper, vitamin E, Centella asiatica extract. Composition according to any one of the preceding claims, the composition further comprising vitamins selected from the group consisting of vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin D, biotin. Composition according to any one of the preceding claims, wherein the composition is free from animal proteins. 11. Non-therapeutic use of a composition according to any one of the preceding claims to reduce oxidative damage to the integumentary system and promote the formation of collagen and elastin.