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IT1403355B1 - METHOD FOR COVALENT IMMOBILIZATION OF ENZYMES ON FUNCTIONALIZED SOLID POLYMER SUPPORTS - Google Patents

METHOD FOR COVALENT IMMOBILIZATION OF ENZYMES ON FUNCTIONALIZED SOLID POLYMER SUPPORTS

Info

Publication number
IT1403355B1
IT1403355B1 ITPD2010A000392A ITPD20100392A IT1403355B1 IT 1403355 B1 IT1403355 B1 IT 1403355B1 IT PD2010A000392 A ITPD2010A000392 A IT PD2010A000392A IT PD20100392 A ITPD20100392 A IT PD20100392A IT 1403355 B1 IT1403355 B1 IT 1403355B1
Authority
IT
Italy
Prior art keywords
enzymes
solid polymer
polymer supports
covalent immobilization
functionalized solid
Prior art date
Application number
ITPD2010A000392A
Other languages
Italian (it)
Inventor
Loris Sinigoi
Lucia Gardossi
Patrizia Spizzo
Diana Fattor
Original Assignee
Univ Degli Studi Trieste
Sprin S P A
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Degli Studi Trieste, Sprin S P A filed Critical Univ Degli Studi Trieste
Priority to ITPD2010A000392A priority Critical patent/IT1403355B1/en
Priority to PCT/EP2011/073794 priority patent/WO2012085206A1/en
Priority to EP11811024.6A priority patent/EP2655611A1/en
Publication of ITPD20100392A1 publication Critical patent/ITPD20100392A1/en
Application granted granted Critical
Publication of IT1403355B1 publication Critical patent/IT1403355B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C12N11/087—Acrylic polymers

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
ITPD2010A000392A 2010-12-23 2010-12-23 METHOD FOR COVALENT IMMOBILIZATION OF ENZYMES ON FUNCTIONALIZED SOLID POLYMER SUPPORTS IT1403355B1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
ITPD2010A000392A IT1403355B1 (en) 2010-12-23 2010-12-23 METHOD FOR COVALENT IMMOBILIZATION OF ENZYMES ON FUNCTIONALIZED SOLID POLYMER SUPPORTS
PCT/EP2011/073794 WO2012085206A1 (en) 2010-12-23 2011-12-22 Method for covalent immobilization of enzymes on functionalized solid polymeric supports
EP11811024.6A EP2655611A1 (en) 2010-12-23 2011-12-22 Method for covalent immobilization of enzymes on functionalized solid polymeric supports

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ITPD2010A000392A IT1403355B1 (en) 2010-12-23 2010-12-23 METHOD FOR COVALENT IMMOBILIZATION OF ENZYMES ON FUNCTIONALIZED SOLID POLYMER SUPPORTS

Publications (2)

Publication Number Publication Date
ITPD20100392A1 ITPD20100392A1 (en) 2012-06-24
IT1403355B1 true IT1403355B1 (en) 2013-10-17

Family

ID=43737201

Family Applications (1)

Application Number Title Priority Date Filing Date
ITPD2010A000392A IT1403355B1 (en) 2010-12-23 2010-12-23 METHOD FOR COVALENT IMMOBILIZATION OF ENZYMES ON FUNCTIONALIZED SOLID POLYMER SUPPORTS

Country Status (3)

Country Link
EP (1) EP2655611A1 (en)
IT (1) IT1403355B1 (en)
WO (1) WO2012085206A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110760495B (en) * 2019-05-07 2023-03-17 宁波大学 Co-crosslinking immobilization method of porcine pancreatic lipase
CN110760499B (en) * 2019-05-07 2023-03-17 宁波大学 Co-crosslinking immobilization method of catalase
US20230159964A1 (en) * 2020-05-07 2023-05-25 Inofea Ag Method to produce biocatalytical compositions
CN112980826B (en) * 2021-02-10 2022-11-04 大连工业大学 Lipase/polyacrylamide hydrogel microsphere catalytic material and preparation method and application thereof
CN113308457B (en) * 2021-05-28 2022-07-29 鲁东大学 Preparation method of covalent organic framework encapsulated enzyme
CN115184299B (en) * 2022-06-01 2024-08-02 广东惠尔泰生物科技有限公司 Method for detecting immobilized lipase enzyme protein content based on near infrared spectrum

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK402583D0 (en) 1983-09-05 1983-09-05 Novo Industri As PROCEDURE FOR THE MANUFACTURING OF AN IMMOBILIZED LIPASE PREPARATION AND APPLICATION
GB9225054D0 (en) 1992-11-30 1993-01-20 Baxenden Chem Enzymatic synthesis
EP0746608B1 (en) 1994-02-21 2003-08-20 Novozymes A/S Method for production of an immobilized enzyme preparation and use of the immobilized enzyme preparation
WO2004024930A2 (en) 2002-09-13 2004-03-25 Suntory Limited Process for production of transesterified oils/fats or triglycerides
ES2214136B1 (en) 2003-02-21 2007-05-16 Consejo Sup. De Invest. Cientificas. NEW IMMOBILIZATION METHOD OF ENZYMES AND OTHER BIO-MACROMOLECULES ON SUPPORTS ACTIVATED WITH EPOXIDE GROUPS CONTAINING IONIZED GROUPS IN THE SPACER ARM THAT JOINS EACH EPOXIDE GROUP TO THE SUPPORT SURFACE.
DE102005030435A1 (en) 2005-06-30 2007-01-04 William Prym Gmbh & Co. Kg mounting sleeve
WO2007080197A2 (en) 2006-01-16 2007-07-19 Novozymes A/S Immobilised enzymes
IL180598A0 (en) * 2007-01-08 2007-07-04 Basheer Sobhi Immobilized interfacial enzymes of improved and stabilized activity
US7790429B2 (en) * 2007-11-28 2010-09-07 Transbiodiesel Ltd. Robust multi-enzyme preparation for the synthesis of fatty acid alkyl esters

Also Published As

Publication number Publication date
WO2012085206A1 (en) 2012-06-28
ITPD20100392A1 (en) 2012-06-24
EP2655611A1 (en) 2013-10-30

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