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IN2015DN02987A - - Google Patents

Info

Publication number
IN2015DN02987A
IN2015DN02987A IN2987DEN2015A IN2015DN02987A IN 2015DN02987 A IN2015DN02987 A IN 2015DN02987A IN 2987DEN2015 A IN2987DEN2015 A IN 2987DEN2015A IN 2015DN02987 A IN2015DN02987 A IN 2015DN02987A
Authority
IN
India
Prior art keywords
experiment
user
dpcr
determining
optimization type
Prior art date
Application number
Inventor
Nivedita Sumi; Majumdar
Thomas; Wessel
David C ; Woo
Marcin; Sikora
Gordon; Janaway
Shweta; Raizada
Joanna; Lankester
Jeffrey; Marks
Daniel; Wessel
Original Assignee
Life Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Corp filed Critical Life Technologies Corp
Publication of IN2015DN02987A publication Critical patent/IN2015DN02987A/en

Links

Classifications

    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B25/00ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
    • G16B25/20Polymerase chain reaction [PCR]; Primer or probe design; Probe optimisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B25/00ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B40/00ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B40/00ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
    • G16B40/10Signal processing, e.g. from mass spectrometry [MS] or from PCR

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Medical Informatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Evolutionary Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Artificial Intelligence (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Data Mining & Analysis (AREA)
  • Databases & Information Systems (AREA)
  • Epidemiology (AREA)
  • Evolutionary Computation (AREA)
  • Public Health (AREA)
  • Software Systems (AREA)
  • Bioethics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Management, Administration, Business Operations System, And Electronic Commerce (AREA)
  • Testing, Inspecting, Measuring Of Stereoscopic Televisions And Televisions (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

A computer- implemented method for designing a digital PCR (dPCR) experiment is provided. The method includes receiving , from a user , a selection of optimization type. The optimization type may be maximizing the dynamic range , minimizing the number of substrates including reaction sites needed for the experiment , determining a dilution factor , or determining the lower limit of detection , for example. The method further includes receiving , from the user , a precision measure for an experiment , and a minimum concentration of a target in a reaction site for the experiment. The method also includes determining a set of dPCR experiment design factors for the experiment based on the optimization type. The set of dPCR experiment design factors is then displayed to the user.
IN2987DEN2015 2012-09-14 2013-09-13 IN2015DN02987A (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US201261701380P 2012-09-14 2012-09-14
US201261714137P 2012-10-15 2012-10-15
US201361758216P 2013-01-29 2013-01-29
US201361788272P 2013-03-15 2013-03-15
US201361830507P 2013-06-03 2013-06-03
PCT/US2013/059815 WO2014043581A1 (en) 2012-09-14 2013-09-13 Methods and systems for a digital pcr experiment designer

Publications (1)

Publication Number Publication Date
IN2015DN02987A true IN2015DN02987A (en) 2015-09-18

Family

ID=49305105

Family Applications (1)

Application Number Title Priority Date Filing Date
IN2987DEN2015 IN2015DN02987A (en) 2012-09-14 2013-09-13

Country Status (11)

Country Link
US (2) US10984888B2 (en)
EP (2) EP2895978A1 (en)
JP (1) JP2016500856A (en)
KR (1) KR20150083992A (en)
CN (2) CN104937600B (en)
AU (1) AU2013315167A1 (en)
BR (1) BR112015005702A2 (en)
IN (1) IN2015DN02987A (en)
RU (1) RU2015113523A (en)
SG (1) SG11201502797PA (en)
WO (1) WO2014043581A1 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8572506B2 (en) 2010-04-09 2013-10-29 Life Technologies Corporation Visualization tool for qPCR genotyping data
US10612080B2 (en) 2014-09-22 2020-04-07 Roche Molecular Systems, Inc. Digital PCR for non-invasive prenatal testing
WO2017205796A2 (en) * 2016-05-27 2017-11-30 Life Technologies Corporation Methods and systems for graphical user interfaces for biological data
CN106906295B (en) * 2017-03-31 2020-12-08 天津诺禾致源生物信息科技有限公司 Method and device for detecting gene point mutation by digital PCR
CN107312850A (en) * 2017-07-19 2017-11-03 华东医药(杭州)基因科技有限公司 A kind of detection method of the invalid amplifications of PCR
CN107784197B (en) * 2017-10-27 2021-01-19 领航基因科技(杭州)有限公司 PCR experiment optimization method
JP7098096B2 (en) * 2017-11-07 2022-07-11 株式会社リコー Detection accuracy identification method, detection accuracy identification device, and detection accuracy identification program
US11198130B2 (en) * 2018-06-21 2021-12-14 Sharp Life Science (Eu) Limited EWOD system and methods to increase dynamic range for digital nucleic acid amplification

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6604092B1 (en) * 1999-02-26 2003-08-05 Lisa E. Stewart Expert system utilizing a knowledge base and design of experiment (DOE) techniques
US20020037507A1 (en) * 1999-12-16 2002-03-28 Walkerpeach Cindy R. Compositions, methods and kits for allele discrimination
WO2003040404A1 (en) * 2001-11-09 2003-05-15 Source Precision Medicine, Inc. Identification, monitoring and treatment of disease and characterization of biological condition using gene expression profiles
DE102005054671A1 (en) * 2005-11-14 2007-05-16 Deutsche Telekom Ag Method and architecture for the application of electronic forms in the medical field
CN101052041A (en) * 2007-05-10 2007-10-10 浙江大学 Integrating engine of medical information
US9487822B2 (en) 2008-03-19 2016-11-08 Fluidigm Corporation Method and apparatus for determining copy number variation using digital PCR
JP5667049B2 (en) * 2008-06-25 2015-02-12 ライフ テクノロジーズ コーポレーション Method and apparatus for measuring analytes using large-scale FET arrays
ES2902894T3 (en) 2008-08-12 2022-03-30 Stokes Bio Ltd Methods for digital PCR
US8285734B2 (en) * 2008-10-29 2012-10-09 International Business Machines Corporation Comparison of documents based on similarity measures
CN101452503A (en) * 2008-11-28 2009-06-10 上海生物信息技术研究中心 Isomerization clinical medical information shared system and method
CN102191314A (en) * 2010-03-12 2011-09-21 孙朝辉 Low-cost, simple and high-efficient detection method of SNP based on PCR
CN101880719B (en) * 2010-07-16 2012-09-05 中国水产科学研究院黄海水产研究所 Microsatellite multi-PCR (Polymerase Chain Reaction) method for turbot paternity test
CN102140541B (en) * 2011-02-15 2012-11-21 北京林业大学 Method for synchronously detecting four viruses of carnation
CN102654891B (en) * 2011-03-03 2015-08-12 深圳市智慧健康产业发展有限公司 A kind ofly cover the health management system arranged of community and family
CN102321624A (en) * 2011-08-30 2012-01-18 天津农学院 Primer sequence for internal reference gene amplification in quantitative PCR (polymerase chain reaction) analysis of gene expression of growth hormone of Clarias leather

Also Published As

Publication number Publication date
WO2014043581A1 (en) 2014-03-20
CN108229104B (en) 2022-06-10
US10984888B2 (en) 2021-04-20
EP2895978A1 (en) 2015-07-22
CN104937600B (en) 2018-01-16
JP2016500856A (en) 2016-01-14
KR20150083992A (en) 2015-07-21
SG11201502797PA (en) 2015-05-28
CN108229104A (en) 2018-06-29
US20150278437A1 (en) 2015-10-01
RU2015113523A (en) 2016-11-10
US20210313010A1 (en) 2021-10-07
CN104937600A (en) 2015-09-23
BR112015005702A2 (en) 2017-07-04
EP4044188A1 (en) 2022-08-17
AU2013315167A1 (en) 2015-04-30

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