HUP0303749A2 - Veserák-specifikus tumormarkerek - Google Patents
Veserák-specifikus tumormarkerek Download PDFInfo
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- HUP0303749A2 HUP0303749A2 HU0303749A HUP0303749A HUP0303749A2 HU P0303749 A2 HUP0303749 A2 HU P0303749A2 HU 0303749 A HU0303749 A HU 0303749A HU P0303749 A HUP0303749 A HU P0303749A HU P0303749 A2 HUP0303749 A2 HU P0303749A2
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Abstract
A találmány tárgyát veserák (<renal cell carcinoma<, RCC)szkrínelésére, diagnózisára és prognózisára, valamint az RCC altípusokazonosítására szolgáló daganatmarkerek képzik. A találmány tárgyátképezi továbbá a daganatmarkerek immunválasz kiváltása céljábólimmunogénként történő alkalmazása, valamint a daganatmarkerek elleniellenanyagok és ellenanyag-fúziós proteinek előállítása céljábóltörténő alkalmazása. Ó
Claims (18)
- -25SZABADALMI IGÉNYPONTOK1. A következők közül legalább egy protein alkalmazása daganatmarkerként: β-aktin, γ-aktin, α-tubulin, citokeratin, citokeratin 8, citoszkeletonbeli tropomiozin, F-aktint fedő protein, hsp 27, hsp 60, hsp 70, hsp 90, grp 78 (BIP), gp 96, glutation-S-transzferáz, glutationszintetáz, szuperoxid-diszmutáz, tioredoxin peroxidáz, PA28a, ubikvitin-tiolészteráz, triózfoszfát-izomeráz, aldóz reduktáz, enoil-CoA hidratáz, α-enoláz, annexin II, IV és V, sztatmin, nikotinamid-N-metiltranszferáz, B23/nukleofoszmin vagy vimentin.
- 2. Az 1. igénypont szerinti protein és/vagy β-tubulin veserák alkalmazása daganatmarkerként.
- 3. Eljárás páciens rákos megbetegedésének in vitro diagnosztizálására és prognosztizálására, azzal jellemezve, hogy a pácienstől nyert szérummintában immun-vizsgálati eljárással kimutatjuk egy 1. vagy 2. igénypont szerinti daganatmarker protein elleni ellenanyag jelenlétét.
- 4. A 3. igénypont szerinti eljárás, azzal jellemezve, hogy az immunvizsgálati eljárás végrehajtása során:a) 1. igénypont szerinti proteint membránon vagy szubsztráton rögzítünk,b) a membránt vagy szubsztrátot egy páciensből származó szérummintával reagáltatjuk,c) a páciensből származó szérummintából daganatmarker-specifikus ellenanyagok jelenlét mutatjuk ki.
- 5. A 4. igénypont szerinti eljárás, azzal jellemezve, hogy a szérummintában lévő daganatmarker-specifikus ellenanyag jelenlétét ellene irányuló, hozzáadott, jelzett ellenanyag alkalmazásával mutatjuk ki.
- 6. A 3-5. igénypontok bármelyike szerinti eljárás, azzal jellemezve, hogy veserákban szenvedő páciens megbetegedésének in vitro diagnosztizálására és prognosztizálására hajtjuk végre.
- 7. Diagnosztikai reagenskészlet, amely alkalmas a 3-6. igénypontok bármelyike szerinti eljárás végrehajtására, és amely reagenskészlet az 1. vagy 2. igénypont szerinti daganatmarkerek közül legalább egyet vagy többet tartalmaz.
- 8. Az 1. vagy 2. igénypont szerinti legalább egy daganatmarker alkalmazása páciens immunválaszának stimulálására szolgáló gyógyszer előállítására.
- 9. Az 1. vagy 2. igénypont szerinti legalább egy daganatmarkerhez immunspecifikusan kötődő ellenanyag vagy fragmense alkalmazása daganatsejtek elpusztítását és/vagy növekedésük meggátlását elősegítő reakciót kiváltó gyógyszer előállítására.
- 10. A 9. igénypont szerinti alkalmazás, amely szerint az ellenanyag vagy annak fragmense ellenanyag-fuziósprotein.
- 11. Gyógyászati készítmény, amely legalább egy 1. vagy 2. igénypont szerinti daganatmarkert, valamint adott esetben gyógyászati szempontból elfogadható vivőanyagot, hígítószert vagy kötőanyagot tartalmaz.
- 12. Gyógyászati készítmény, amely az 1. vagy 2. igénypont szerinti daganatmarkerek legalább egyikéhez immun-specifikusán kötődő ellenanyagot vagy ellenanyag-fragmenst, valamint adott esetben gyógyászati szempontból elfogadható vivőanyagot, hígítószert vagy kötőanyagot tartalmaz.
- 13. A 12. igénypont szerinti gyógyászati készítmény, amely ellenanyagként vagy ellenanyag-fragmensként ellenanyag-fuziósproteint tartalmaz.
- 14. A 11-13. igénypontok bármelyike szerinti gyógyászati készítmény, amely kemoterápiás ágenst is tartalmaz.
- 15. Gyógyászati készlet, amely egy első tárolóedényben a 11-13. igénypontok bármelyike szerinti gyógyászati készítményt, egy második tárolóedényben pedig egyidejű vagy időben eltolt beadásra alkalmas, kemoterápiás ágenst magában foglaló gyógyászati készítményt tartalmaz.
- 16. Az anti-citokeratin 8, anti-vimentin és anti-sztatmin ellenanyagok közül legalább egy alkalmazása veserák immunhisztokémiai eljárásokkal történő azonosítására és veserák altípusok elkülönítésére.
- 17. Eljárás veserák azonosítására és veserák altípusok elkülönítésére, azzal jellemezve, hogy:a) veserák-gyanús pácienstől nyert vesehám-szövetmintát az ellenanyag szövetmintához történő kötődését lehetővé tevő körülmények között a következő ellenanyagok közül legalább eggyel inkubálunk: anti-citokeratin 8, anti-vimentin vagy anti-sztatmin (első ellenanyag);b) az első ellenanyagot az első ellenanyagra vonatkozó kötődési affinitással rendelkező felismerési helyet és a fentiekben ismertetett kimutatást elősegítő jelzőanyagot tartalmazó második ellenanyaggal a második ellenanyag első ellenanyaghoz történő kötődését lehetővé tevő körülmények között inkubáljuk;3 “** • ··· · ·♦ ···-27- ........c) egy detekciós lépéssel kimutatjuk az első ellenanyaghoz kötődött második ellenanyagot;d) a c) pontban vizsgált szövetmintákat összehasonlítjuk világos sejtes, kromofób, kromofil vagy onkocitómás veserák altípusokban szenvedő betegektől nyert referencia-5 mintákkal.
- 18. Reagenskészlet veserák azonosítására és veserák altípusok elkülönítésére, amely a következőket tartalmazza:a) legalább egy 16. igénypont szerinti ellenanyag (első ellenanyag),b) az első ellenanyag elleni, kimutatást elősegítő jelzőanyagot hordozó második ellen-10 anyag.A meghatalmazott: DANUBIASzabadalmi és Védjegy Iroda Kft.dr. Pethő Árpád szabadalmi ügyvivő
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- 2002-03-28 CZ CZ20032787A patent/CZ20032787A3/cs unknown
- 2002-03-28 HU HU0303749A patent/HUP0303749A3/hu unknown
- 2002-03-28 WO PCT/EP2002/003503 patent/WO2002082076A2/en not_active Application Discontinuation
- 2002-03-28 CA CA002442957A patent/CA2442957A1/en not_active Abandoned
- 2002-03-28 RU RU2003130645/15A patent/RU2003130645A/ru not_active Application Discontinuation
- 2002-03-28 BR BR0208603-4A patent/BR0208603A/pt not_active Application Discontinuation
- 2002-03-28 MX MXPA03009018A patent/MXPA03009018A/es unknown
- 2002-03-28 KR KR10-2003-7013015A patent/KR20030086345A/ko not_active Withdrawn
- 2002-03-28 US US10/473,601 patent/US20040096916A1/en not_active Abandoned
- 2002-03-28 EP EP02759781A patent/EP1373900A2/en not_active Withdrawn
- 2002-03-28 SK SK1287-2003A patent/SK12872003A3/sk unknown
- 2002-03-28 JP JP2002579795A patent/JP2004531713A/ja not_active Withdrawn
- 2002-03-28 PL PL02363009A patent/PL363009A1/xx unknown
- 2002-03-28 CN CNA028068661A patent/CN1630819A/zh active Pending
-
2003
- 2003-10-30 ZA ZA200308487A patent/ZA200308487B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| WO2002082076A3 (en) | 2003-09-04 |
| RU2003130645A (ru) | 2005-04-10 |
| HUP0303749A3 (en) | 2005-09-28 |
| KR20030086345A (ko) | 2003-11-07 |
| SK12872003A3 (sk) | 2004-02-03 |
| CA2442957A1 (en) | 2002-10-17 |
| PL363009A1 (en) | 2004-11-15 |
| MXPA03009018A (es) | 2004-02-12 |
| ZA200308487B (en) | 2005-01-31 |
| BR0208603A (pt) | 2004-03-02 |
| US20040096916A1 (en) | 2004-05-20 |
| EP1373900A2 (en) | 2004-01-02 |
| CN1630819A (zh) | 2005-06-22 |
| JP2004531713A (ja) | 2004-10-14 |
| CZ20032787A3 (en) | 2004-03-17 |
| WO2002082076A2 (en) | 2002-10-17 |
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