HU210157B - Process for extracting of flowered branches or leaves of hawthorn - Google Patents

Abstract

The invention relates to an extraction process for the production of hawthorn extracts. According to the present invention, the cut Hawthorn herb is subjected to a pretreatment prior to extraction by suitably adjusting the salt content and pH of the water-alcohol solvent with the inorganic and organic compounds selected, and then pectinase enzyme is excreted into the system. the surface of the herb and the pre-treated herb is extracted with a solvent. EN 210 157 Scope of the description: 4 pages

Description

BACKGROUND OF THE INVENTION The present invention relates to a novel process for the preparation of hawthorn herb extracts by treating the herbaceous plant with a pectin-degrading enzyme in a suitably adjusted salt concentration and pH to dissolve the surface, using a pre-treated herb as a solvent.

From herbal tea to cosmetics to herbs, herbs serve as ingredients in many areas. The herb itself, its leaves, stems, fruits, flowers, etc. can be used.

The active ingredients of the herbs are usually extracted by solvent soaking and extraction, not infrequently by alcohol-water mixtures. Both strictly prescribed methods (such as Ph. Hg. VII.) Or generally known routine methods for preparing herbal extracts and extracts are known.

In the literature, folk medicine and In the methods described in cosmetic practice, the variable parameter is usually the component composition of the extraction solvent, the temperature, and the soaking time. It can be stated that these methods provide essentially the same extracts, which has the disadvantage that many of the ingredients remain in the herb.

In other words, the known standard procedures are about to "give up" the recovery of certain active ingredients, which are thus lost in their uses.

The above reasoning is especially true for herbs that use the nutty, tincture of the nuts to make extracts. The hard shell, the shell, makes it difficult or impossible for the solvent to penetrate, so that materials inside are not, partially or with poor efficiency.

The aim of the present invention is to extend the known and routinely used herbal extraction process possibilities by developing a new method, which enables to obtain active substances which were not previously available, and to improve the efficiency and economy of the extraction.

It has been found that by loosening the surface of a nutty herb with a hard outer layer by enzymatic digestion under suitably chosen conditions, the extraction rate may be increased, the composition of the resulting extract may be changed, and new substances may be detected which are not from the herb to the extract.

In our experiments we found that pretreatment, loosening and digestion of the surface layer should be carried out with enzymes that break down the cell wall of plants (pectinase, cellulose, etc.).

However, according to our observations, the fact that when the inorganic and / or organic salts (phosphates, citrates) are dissolved in the solvent used for the enzymatic pre-treatment and the proper pH is achieved, the extraction efficiency is determined by salinity and pH, extraction temperature, time, etc. played only a secondary role.

Thus, it has been found that the extraction of herbs can be significantly influenced by the use of some, e.g. pectin-degrading enzymes are pre-soaked in salt concentration and pH adjusted.

We will illustrate the above with a practical example.

In the case of hawthorn (Crataegus oxycantha), the flowering branch or leaf was subjected to aqueous-alcoholic extraction to show that various amines, essential oils, etc. (Isépy: Herbs, Rácz, G .: Herbal Science, p. 138, Varró, A.B: Herbal Medicinal Effects, p. 96), the drug can be used internally as well as externally.

If the extraction of hawthorn is not carried out by the direct method described above, first the salt content and pH are adjusted by addition of disodium hydrogen phosphate, sodium hydroxide or citric acid to a water-alcohol mixture, and the hawthorn is soaked for 1 hour in the presence of pectinase enzyme. pre-treated hawthorn for extraction, the resulting drug has a significantly darker color and a higher dry matter content. gas chromatography, infrared spectroscopy and mass spectrometry showed a different composition compared to untreated.

The present invention therefore relates to a novel process for preparing hawthorn extracts by subjecting the herb to a surface-loosening pretreatment in the presence of a pectin-degrading enzyme in a suitable salt concentration in a pH-adjusted solvent.

The advantages of this new process are many:

- the pre-treatment modifies the composition of the herbal extract, the drug

- extracting new components

- the extraction efficiency is improved

- the method is simple, the pre-treatment conditions can be varied flexibly with the herb, enzyme, etc. depending on.

The process of the invention is illustrated by the following examples:

/. example (basic procedure - comparator, no pretreatment)

Cut the hawthorn leaf into pieces of at least 1 cm or. spread the flowering branch evenly over a sapwood plate, then pour 30 g of herbs first with 1 liter of water heated to 90 ° C, then 1 liter of room temperature, leave the water to drain and cool the herb.

The herb, which has cooled to room temperature, is transferred from the sital plate to a vessel and 1.1 liters of 50% alcohol are added and stirred twice daily for 6 days and then filtered.

HU 210 157 Β

Extract solids content: = reference base: 100%.

Example 2

30 g of hawthorn leaf or chopped into pieces of at least 1 cm. spread the flower branch on a sapwood plate and wash with 1 liter of water at 90 ° C and then 1 liter at room temperature.

The herb, which has been cooled to room temperature, is transferred to a vessel, 0.5 l of 50% alcohol and 1 ml of pectinase enzyme (commercially available from Phylazime) are added. The suspension was allowed to stand for one day, then filtered and washed with 0.5 L of water. The pre-treated herb is soaked in a vessel with 1.1 L of 50% alcohol (twice a day) for 6 days and filtered. The drug thus obtained is slightly darker in color than that obtained in Example 1, having a dry solids content of 105% (average of four experiments).

Evaluation: Pre-treatment with pectinase slightly increases the dry matter content of the extract (improved extractability) and analytical analyzes also show differences in composition.

Example 3

The procedure of Example 2 was used except that 2.4 g of disodium hydrogen phosphate in 0.5 L of water for pre-treatment 50% alcohol and 19.2 g of 1.5 L of IN sodium hydroxide were used. citric acid, followed by pectinase enzyme, followed by 1 day enzyme digestion.

Extraction of the herb treated with the thus prepared digestion solution yielded a drug having a dry matter content of 110%.

Rating: Compared to the reference, there is hardly any change in pretreatment compared to Example 2.

Example 4

The procedure of Example 3 was followed except that 2.2 g of adenosine were added to the digestion solution during the pretreatment.

The drug content obtained during the extraction of the herb so treated is 100%, ie no significant difference can be detected.

Example 5

The procedure is the same as described in Example 2, except that 2.2 g of disodium adenosine monophosphate is added to the pretreatment solution.

The drug obtained by the extraction of the herb so treated has a darker color, analytical results show a significantly different composition and a dry matter content of 120%.

Evaluation: The effect of enzyme treatment changed significantly by changing the salt composition.

Example 6

Hawthorn wash and wash. the pre-treatment extraction is the same as in Example 2.

Procedure to prepare the pretreatment-digest solution:

Dissolve in 1.5 L of 2N sodium hydroxide solution

19.2 g citric acid.

2.37 g of disodium hydrogen phosphate are dissolved in 0.5 l of water and added to the citric acid solution.

Dissolve 2.8 g of sodium chloride and 2.2 g of disodium adenosine monophosphate in 2 liters of deionized water and add to the previous solution and adjust the pH to 7.3 with sodium hydroxide, and then add the pectinase enzyme. soak in the digestion solution and add the pectinase enzyme - then soak in the digestion solution for one day.

Extraction of the hawthorn pretreated in this way yields a dark brown drug with a change in composition of 135% dry matter.

If the solvent composition of the pretreatment solution is changed (water: alcohol ratio, KOH instead of NaOH), or the temperature is between 5 and 50 ° C. the pre-treatment time between 10 minutes and 7 days, the differences obtained are considered to be the same within the margin of error.

The following examples are summarized in the following table:

Example track of today Components added during pre-treatment Dry- material content pectinase Salt pH adjusting, Bending Other adenine origin derivative 1 - - - - Base = 100% 2 + - - - 105 3 + + - (-) ' 110 4 + + + (-) ' 110 5 + + + (-) ' 120 6 + + + + 135

¹ separate pH adjustment was not made, only the base citric acid buffer prevails

Claims (2)

A process for preparing hawthorn extracts by solvent extraction of the herbaceous plant after flowering branch or leaf after enzymatic pre-treatment of pectin, characterized in that the enzymatic pre-treatment is pectinase at a room temperature of 5 to 50 ° C for 1 hour to 1 week, preferably 1 day. the digestion enzyme digestion and the aqueous solution used for this purpose, preferably 50% by weight of ethyl alcohol solvent, has a salt content ΙΟΙ 00 g / l, preferably 30 g / l, and its pH is 7-8, preferably To 7.3 using inorganic and / or organic compounds. 2. The process of claim 1, wherein the inorganic compound is sodium or potassium hydroxide, disodium hydrogen phosphate, and the organic compound is citric acid, adenine and derivatives thereof for adjusting the salt and pH.