HU191489B - Cosmetic containing living unicellular alga - Google Patents

Abstract

Process for producing skin care preparations, such as medicinal preparations, particularly preparations which have an anti-inflammatory activity, by adding to the conventional carrier elements and/or other additives of skin care preparations parts of tissues or living plant cells, particularly algae cells to be used as active elements.

Description

The present invention relates to a cosmetic composition comprising living single-cell algae as an active ingredient.

There is a growing worldwide interest in cosmetic formulations containing naturally occurring substances as active ingredients. Such a group of cosmetic products is called so-called. phytocosmetics containing as an active ingredient extracts of various herbs such as herbs, vegetables or fruits. More recently, algae extracts are also used as active ingredients. Thus, British Patent No. 1,392,131 discloses cosmetic compositions containing nucleoplasm of marine plankton, such as Astcrionclla japonica, Chactoccros ceres. German Patent No. 2 532 934 relates to cosmetic compositions containing cell-free extracts of mixed plankton in sulfur-containing thermal waters. German Patent No. 2,940,411 discloses extracts of dry matter of Scenedesmus green algae pure for medicinal purposes. A common feature of these solutions is the use of dead cells, extracts of disintegrated cells, or fractions of specific nuclei.

The present invention is based on the discovery that when intact, living plant cells such as algae cells are used as the active ingredient of cosmetic compositions instead of dead plant cells, the skin care compositions thus produced exhibit antiinflammatory, hydrating and circulatory effects and smooth the epidermis of the face.

Accordingly, the present invention relates to cosmetic compositions containing 0.005 to 2.5% by weight of live unicellular algae of the strain Chlorophyta, Chroinophyta, Xanthophyta or Chrysophyta (according to P. Bourelly, H. Ettl and J. Schultze-Motel) as the usual carrier. - and mixed with excipients.

Algae cells are obtained from pure bred algae prepared under sterile conditions, preferably from pure algae species. Particularly preferred algae are Euchlorophyceae sp., Botryochloridaceae sp., Zygophyceae sp., Chlorococcales sp. and Crysosphaerales sp. algae of species such as Scenedesmus quadricauda, Selenastrum capricornutum and Chlamydonomas debaryna.

The algae are cultivated in the usual manner under sterile or aseptic conditions and then added to the base of the cosmetic composition containing the usual carriers and excipients, after they have reached the appropriate stage of development.

The carriers used in the compositions of the invention may be fat or oil-like. These are the most important additives in cosmetic formulations used primarily in emulsified form. Their main role is to replace "lost" fat in the skin. Such carriers include animal fats and waxes such as lanolin, cetacea, beeswax, lard, cod liver oil, cetole oil, fatty alcohols such as cetyl alcohol, lauryl alcohol, myristyl alcohol, fatty acids such as pala-lauric acid, palmitic acid, stearic acid, vegetable fats and oils such as avocado oil, wheat germ oil, corn germ oil, sunflower oil, ricin10 oil, glycerin, glycerol, a .

In addition to excipients, emulsifiers, preservatives, anticancer agents, disinfectants, perfumes, alkaline agents, water, and water-binding agents may be used.

The emulsifier may be, for example, cholesterol, lecithin or cholesterol vaseline (eucerin).

The purpose of the application of preservatives is to preserve the original quality and properties of the different materials and the finished product for as long as possible. In cosmetic products, the deterioration of quality is most often caused by microorganisms, which can cause pimples, rot, mold. An ideal preservative is one that is colorless, odorless and non-irritating to the skin, is effective in small amounts and does not react with the ingredients of the formulation. Examples of such preservatives are salicylic acid, benzoic acid, p-oxybenzoic acid and alkyl or aryl esters thereof.

The anti-fouling agents are added to prevent the fattening of vegetable and animal fats. Such materials include antioxidants such as hydroquinone, tocopherols, gallic acid, butylhydroxytoluene and butylhydroxyanisole.

Perfumes are used by the cosmetics industry to mask the odor of the carrier. They evaporate at ordinary temperatures and are slightly bactericidal. Examples of such fragrances are lavender oil, lemon oil, and synthetic fragrances, which have the advantage of having constant physico-chemical parameters.

Alkali agents are used, in part, for emulsification, saponification, and degreasing, for example, triethanolamine, borax.

Water is one of the most important ingredients in cosmetics. Distilled water is used in cosmetic formulations since some emulsions are sensitive to the electrolyte content of the water.

The purpose of using water-binding agents is to prevent the water from separating from the emulsion. Examples of such materials are glycerol, sorbitol and glycols.

The disinfectant may be, for example, alum, boric acid.

The formulations may be, for example, creams, solutions or lotions.

The compositions are prepared in a known manner.

The invention will now be illustrated by the following exemplary embodiments.

Example 7

Production of algae cells

Zehnder Z-8 medium (Publication B-1/69, Norwegian Institute for Water Research, Blindern, 1972) was aseptically inoculated with sterile inoculum from Scenedesmus quadricauda pure culture. The initial concentration is 1000 cells / ml. Cultures were continued for 14 days at room temperature with 4000 lux illumination, alternating 12 hours dark and 12 hours illuminated. 7 and

Between day 14, daily, half of the culture is decapitated, pelleted, and re-original

Filled to 191,489 teeth. Algae cells seeded on days 7-14 are used in the formulations shown in the following examples.

grace Chod., Scenedesmus subspieatus Chod. species of algae are used.

Example 2 Moisturizing cream Component A Component A The amount (Wt%) lanoline 3.6 cetyl alcohol 3.0 stearin 6.1 cholesterol 2.0 corn germ oil 5.0 glycerol 7.0 triethanolamine 0.1 distilled water 70.0 Nipagin (methyl p-oxybenzoate) 1.0 BHT (Butylhydroxytoluene) 0.1 Scenedesmus quadricauda 2.0 (Cont.) Bréb. active ingredient fragrance 0.1

The moisturizing cream is also prepared using algae of the species Scenedesmus obtusiusculus Chod., Scenedesmus balatonicus Hortob., Scenedesmus ecornis, Chodatella quadriseta Lemm., Selenastrum gracile Reinsch., Chlorella ellipsoidea.

Example 3 Cream for normal skin Component A Component A The amount (I%) stearin 7.2 lanoline 6.0 cetyl alcohol 6.3 cetviasz 4.0 corn germ oil 5.8 eucerin (cholesterol vaseline) 4.2 cholesterol 2.6 glycerol 6.8 distilled water 56.0 nipagin 0.2 triethanolamine 0.2 BHT Chlorella pyrenoidosa Chick 0.1 agent 0.5 fragrance 0.1

Normal skin cream is also prepared by the action of Scenedesmus quadricauda (Turp.) Bréb., Ankstrodesmus angustus Bern., Scenedesmus acuminatus (Lagerh.) Chod., Scenedesmus intermedius, Scenedesmus acutiformis Schröder, Scenedesmus.

Example 4 Cream for dry skin Component A Component A The amount (Wt%> cetviasz 7.0 lanoline 9.1 cetyl alcohol 2.0 cholesterol 2.0 corn germ oil 18.4 glycerol 8.4 distilled water 50.0 nipagin 0.2 triethanolamine 0.25 BHT Scenedesmus obliquus (Turp.) Kütz. 0.05 agent 2.5 fragrance 0.1

Oily skin cream is prepared, except that the active ingredient is Scenedesmus quadricauda (Turp.) Bréb., Pediastrum duplex Meyen, Scenedesmus acutus Meyen, Scenedesmus obliquus (Turp.) Kütz, Chlorella pyrenoidosa Chick, Chlorella vulgaris Beijer.

Component A Example 5 Cream for oily skin Component amount (%) cetviasz 2.0 stearin 18.0 cetyl alcohol 4.7 Wheat germ oil 10.0 glycerol 8.0 distilled water 55.0 nipagin 1.0 triethanolamine 0.1 BHT 0.1 Botrichloris simplex Pascher agent 1.0 fragrance 0.1

Dry skin cream is prepared, except that the active ingredient is Scenedesmus quadricauda (Turp.) Bréb., Oocystis lacustris Chod., Kirch. lunaris (Kirchn.) Möb., Pediastrum tetras (Ehrb.) Ralfs, Closterium venus Kg. species of algae are used.

191,489

Component A Example 6 Body lotion Component amount (%) lanoline 3.0 cetyl alcohol 3.0 stearin 5.0 propylene glycol 1.9 distilled water 85.0 nipagin 0.1 BHT 0.1 triethanolamine 0.3 Pediastrum duplex Meyen agent 1.5 fragrance 0.1

A personal care composition is prepared comprising the active ingredient Scenedesmus quadricauda (Turp.) Bréb., Chodatella balatonica Scherf., Pediastrum boryanum (Turp.) Mcncgh., Closterium ehrenbergii Mcnegh., Closterium balatonicum Hortob. species of algae are used.

Example 7 Component A Face milk Quantity of the component lanoline (Wt%) 3.0 stearin 2.0 cetyl alcohol 3.0 corn oil 10.0 distilled water 80.0 triethanolamine 0.4 BHT 0.5 nipagin 0.5 The active ingredient is Chrysosphaera paludosa 0.05 iiiatanyag 0.1

Facial milk is also prepared using algae of the species Scenedesmus quadricauda (Turp.) Bréb., Botryochloris chlorellidiopsis Ettl, Botryochloris simplex Paschcr, Helcrodesmus multicellularis Wawrik.

The algae species used in the compositions were determined, in addition to Bourelly's already mentioned work, by the following works: Ettl, H., J. Gerloff, H. Heynig, Süsswasserflora von Mitteleuropa, Bánd 3: Xanthophyceae 1. (H. Ettl) 1978. Gustav Fischer Veri. Jena; Uherkovich G .: Die Scenedesmus-arten Ungarns, 1966. Akadémiai Kiadó, Budapest.

The Scenia designation used in the following efficacy studies refers to the compositions of the present invention.

Testing the efficacy of the compositions of the invention

In 1983 we started experimenting with Scenia creams. We worked with 50 patients, of course on different skin types.

Particularly sensitive skins were collected. After consulting a biologist, it has been found that the anti-inflammatory cooling of the complex herbal active ingredient can be expected.

Patients were divided into two groups; treated (previously regularly treated skins) and untreated skins.

After ten days, in both variants, the skins were found to have a velvety feel, but no other result was found. After two to three weeks, it was reported that besides the velvety palate, “skin hydration, ie fullness of the skin, had increased. His painful tension had ceased.

The biomolecules selected by the algae "work" in a natural, high-water trap. So, a lotion had to be made that suited both the algae and the different skin types.

We relied on transdermal absorption to make our creams from easily absorbable materials. This is the first time that anti-inflammatory effects were present. The complaints were: dry, scaly patches on the face, prone to erythema-prone skin, also with dry patches.

Our biggest surprise was that we also saw a significant improvement in patients with perioral dermatitis. After a few daily creams, the complaints disappeared.

After that, the most sensitive skin was selected and the creams were applied without active ingredient. However, after a few days, the older complaints returned. After repeated application of the cream of the invention, they reappeared after a few days:

good hydration, velvety touch, disappearance of itchy spots.

The anti-inflammatory effect was observed in alcohol, weather-sensitive patients, minor dermatitis from internal disorders, cracked hands, itchy, painful stretching skin, with very good results.

The results of some specific treatments are listed below.

1.36 Year Old Patient: Deep water deficiency, deficient skin, surgical (gynecological) aftermath, with erythematous patches on the nasal bridge, we started using the cream in November 1983. After 2 weeks the red spots disappeared and the patient spoke of a pleasant velvety feeling. After a few months of use, the cream was withdrawn and the patient was uncomfortable pulling. he complained of a tight, itchy feeling. After using the cream again, the feeling of being good again.

2. 43-year-old patient: Dry, deep-water-sensitive, sensitive skin. Responds to excitement with strong erythemia. Has been using the cream since October 1983. The result: a very pleasant feeling, soft, soft to the skin, well hydrated, erythcmia stopping. Returned for inspection in the summer of 1985. Use the cream regularly and your skin will always feel good.

191,489-year-old office worker: Water-depleted, deep-water and fat-deficient skin. It responds to cold, warm (spirits, vasodilators) with powerful erythemia. He started using the cream in October 1983. We asked for it in four weeks. Result: velvety skin, good hydration, pleasant feeling. Since then, use the cream with good results.

year old teacher: Kt 'Pásás seborhea with some acne, very sensitive to weather changes. In October 1983 he received a moisturizing cream. We ordered it back after three weeks. Condition: Quite surprising, velvety skin, ι dandruff has completely disappeared. The skin became evenly colored and shiny.

Yearly Sugar :: Combination skin, swollen on the seborheas area, itchy, dry, reddish on both sides of the face. He received a moisturizing cream in October 1983, and was refunded after two weeks. The result: the dry itching sensation was completely eliminated, the redness was strongly reduced, and the skin was well hydrated and velvety to the touch. After two months of use, the cream was changed without the guest's knowledge. Result: the skin recurred, itchy discomfort and redness at the site reappeared. Complaints for the use of algae cream have ceased. Since then he has been using algae cream continuously with good results.

years old restaurant manager: Oily seborheas skin. The face was regularly treated with alcohol ».. Tense, shiny, itchy skin Prone to vasodilatation, exacerbated by the use of alcohol. Kidney has undergone lung surgery and is a systemic and chronic pain in ovarian inflammation. He received an algae moisturizing cream in October 1983. Result: The discomforting, itchy sensation was completely eliminated. Your skin is well hydrated, and comedians are loose, easy to remove during cosmetic treatment. The skin is very soft, soft to the touch, velvety. The cream is still being used successfully by the patient.

year old technical drawer: Dry seborheas skin but prone to acne due to chronic ovarian inflammation. He scratches it permanently, so his face is dotted with permanent pigment spots. He received an algae moisturizing cream. Result: The exfoliation on the skin is completely eliminated, there are no acne, only the signaling acne before the menses. Soft, velvety skin. He probably won't scratch because of the itching, so there are no new pigment spots.

We did not use the cream for the regeneration or removal of acne, but the redness left after cleaning them was treated successfully.

In the past, drying pastes were used to remove traces of the treatment, which we replaced with a cream called "oily skin". After a day or two, the remaining redness receded or disappeared. Particularly good results have been achieved in sensitive, inflammatory skin. We also tested rosaceas on skins, which were accompanied by a lack of surface water. After a few daily use, the surface water deficiency decreased and the spots associated with rosacea localized. The feeling and touch was pleasant.

The treated subject had a 49-year-old hereditary predisposition to consuming large quantities of coffee and alcohol.

For Seborheas skins, we tried the creams, which were highly dandruffy, dry, reddish-prone skin types. They were given a moisturizing cream. (30-year-old saleswoman, 18-year-old shop assistant, 35-year-old office worker, 31-year-old receptionist, 40-year-old educator.) After a few weeks of use, we received very good skin that became velvety and symptoms disappeared.

In addition to our dry skin cream and our extra-fat cream, we have found good results with deep-water and non-greasy skin.

We also performed comparative studies on cosmetic treatments. The comparison was made from several points of view. On the one hand, the composition according to the invention was compared with the cosmetics of the Austrian Edit Pichler (Wien 13, Lainzer Str. 17), which also contains algae but in the form of seaweed and dried, dried. On the other hand, we compared the treated and untreated sides of the face. '

Part of the observation focused on cosmetic treatments. Here we put different wraps on both sides of the face. Patients treated with the composition of the present invention, after washing the cream (pack), had a much more velvety palate, better hydration than the comparative cream pack, when no velvety palpation was felt.

In addition, experiments have been conducted in dry individuals prone to erythema, where in some cases inflammation has already occurred. We distributed the cream to four or four individuals. The cream of the invention eliminated the inflammation and red spots within two days, eliminating the discomforting tenderness. On the other four faces treated with a cream containing no active ingredient, inflammation persisted, the stretching, tense feeling was reduced, and the skin became more hydrated, but the velvety sensation of the product of the present invention was not observed.

Claims (1)

Cosmetic composition containing algae as active ingredient, characterized in that it contains from 0.05% to 2.5% by weight, based on the total weight of the composition, of live unicellular algae of the strains Chlorophyta, Chromophyta, Chrysophyta or Xanthophyta, preferably from 11% to 35% by weight. , cetyl alcohol, 0.1-0.4% emulsifier, preferably cholesterol, 0.1-1.01% preservative, preferably p-oxybenzoic acid methyl ester, 0.05-0.5% rancidity an inhibitor, preferably butylhydroxytoluene, 60.6-88.6% water, optionally 0.1% perfume.