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HRP20220771T1 - Postupak enzimske hidrolize lignoceluloznog materijala i fermentacije šećera - Google Patents

Postupak enzimske hidrolize lignoceluloznog materijala i fermentacije šećera Download PDF

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Publication number
HRP20220771T1
HRP20220771T1 HRP20220771TT HRP20220771T HRP20220771T1 HR P20220771 T1 HRP20220771 T1 HR P20220771T1 HR P20220771T T HRP20220771T T HR P20220771TT HR P20220771 T HRP20220771 T HR P20220771T HR P20220771 T1 HRP20220771 T1 HR P20220771T1
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HR
Croatia
Prior art keywords
lignocellulosic material
alcohol
enzymatic hydrolysis
producing microorganism
fermentation
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Application number
HRP20220771TT
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English (en)
Inventor
Johannes Petrus Smits
Original Assignee
Dsm Ip Assets B.V.
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Publication date
Application filed by Dsm Ip Assets B.V. filed Critical Dsm Ip Assets B.V.
Publication of HRP20220771T1 publication Critical patent/HRP20220771T1/hr

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/145Fungal isolates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • C12P7/08Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/645Fungi ; Processes using fungi
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00Technologies relating to chemical industry
    • Y02P20/10Process efficiency

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Claims (14)

1. Integrirani postupak za proizvodnju alkohola iz lignoceluloznog materijala, naznačen time što se postupak sastoji od: - predobrade lignoceluloznog materijala radi dobivanja prethodno obrađenog lignoceluloznog materijala, - enzimske hidrolize prethodno obrađenog lignoceluloznog materijala da se dobije enzimski hidrolizirani lignocelulozni materijal, pri čemu se kisik dodaje tijekom enzimske hidrolize, - razmnožavanje mikroorganizma koji proizvodi alkohol fermentacijom dijela prethodno obrađenog lignoceluloznog materijala i fermentacijom enzimski hidroliziranog lignoceluloznog materijala mikroorganizmom koji proizvodi alkohol radi proizvodnje alkohola, pri čemu mikroorganizam koji proizvodi alkohol može fermentirati barem C5 šećer i barem C6 šećer, - razmnožavanje gljivice i proizvodnje enzima pomoću gljivice, pri čemu se dio prethodno obrađenog lignoceluloznog materijala koristi za razmnožavanje gljivice i/ili proizvodnju enzima pomoću gljivice i pri čemu se enzimi koje proizvodi gljivica koriste u enzimskoj hidrolizi.
2. Postupak prema zahtjevu 1, naznačen time što se dio enzimski hidroliziranog lignoceluloznog materijala i dio lignoceluloznog materijala koristi za razmnožavanje mikroorganizma koji proizvodi enzim i/ili proizvodnju enzima od strane mikroorganizma koji proizvodi enzim.
3. Postupak prema patentnom zahtjevu 1 ili 2, naznačen time što enzimska hidroliza sadrži barem: - korak ukapljivanja pri čemu lignocelulozni materijal hidrolizira u najmanje prvom spremniku, i - korak saharifikacije pri čemu ukapljeni lignocelulozni materijal hidrolizira barem u prvom spremniku i/ili barem u drugom spremniku.
4. Postupak prema bilo kojem od patentnih zahtjeva 1 do 3, naznačen time što se alkohol, enzimi, mikroorganizam koji proizvodi enzim i/ili mikroorganizam koji proizvodi alkohol obnavljaju.
5. Postupak prema bilo kojem od patentnih zahtjeva 1 do 4, naznačen time što se lignocelulozni materijal prije enzimske hidrolize podvrgava najmanje jednom odvajanju krutina/tekućina.
6. Postupak prema bilo kojem od patentnih zahtjeva 1 do 5, naznačen time što se enzimski hidrolizirani lignocelulozni materijal podvrgava najmanje jednoj separaciji krutina/tekućina.
7. Postupak prema bilo kojem od patentnih zahtjeva 1 do 6, naznačen time što je gljivica Rasamsonia.
8. Postupak prema bilo kojem od patentnih zahtjeva 1 do 7, naznačen time što su enzimi u obliku cijele smjese za fermentaciju.
9. Postupak prema bilo kojem od patentnih zahtjeva 1 do 8, naznačen time što je mikroorganizam koji proizvodi alkohol kvasac.
10. Postupak prema patentnom zahtjevu 9, naznačen time što je mikroorganizam koji proizvodi alkohol Saccharomyces cerevisiae.
11. Postupak prema bilo kojem od patentnih zahtjeva 1 do 10, naznačen time što sadržaj suhe tvari na kraju enzimske hidrolize iznosi 5 mas.% ili više.
12. Postupak prema bilo kojem od patentnih zahtjeva 1 do 11, naznačen time što je alkohol etanol.
13. Postupak prema bilo kojem od patentnih zahtjeva 1 do 12, naznačen time što su enzimska hidroliza i fermentacija odvojeni koraci.
14. Postupak prema bilo kojem od patentnih zahtjeva 1 do 12, naznačen time što se kombiniraju enzimska hidroliza i fermentacija.
HRP20220771TT 2015-01-28 2016-01-27 Postupak enzimske hidrolize lignoceluloznog materijala i fermentacije šećera HRP20220771T1 (hr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP15152901 2015-01-28
EP19204992.2A EP3640336B1 (en) 2015-01-28 2016-01-27 Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars

Publications (1)

Publication Number Publication Date
HRP20220771T1 true HRP20220771T1 (hr) 2022-09-16

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Family Applications (2)

Application Number Title Priority Date Filing Date
HRP20220771TT HRP20220771T1 (hr) 2015-01-28 2016-01-27 Postupak enzimske hidrolize lignoceluloznog materijala i fermentacije šećera
HRP20192221TT HRP20192221T1 (hr) 2015-01-28 2019-12-11 Postupak enzimske hidrolize lignoceluloznog materijala i fermentacije šećera

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Country Status (12)

Country Link
US (3) US10435719B2 (hr)
EP (3) EP3250697B1 (hr)
CN (1) CN107429265A (hr)
CA (1) CA2973303C (hr)
DK (2) DK3250697T3 (hr)
ES (1) ES2767554T3 (hr)
HR (2) HRP20220771T1 (hr)
HU (1) HUE048346T2 (hr)
MY (2) MY196163A (hr)
PL (2) PL3250697T3 (hr)
SI (1) SI3250697T1 (hr)
WO (1) WO2016120297A1 (hr)

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