HRP20201617T1 - Medij kulture za epitelijalne matične stanice i organoidi koji se sastoje iz spomenutih matičnih stanica - Google Patents
Medij kulture za epitelijalne matične stanice i organoidi koji se sastoje iz spomenutih matičnih stanica Download PDFInfo
- Publication number
- HRP20201617T1 HRP20201617T1 HRP20201617TT HRP20201617T HRP20201617T1 HR P20201617 T1 HRP20201617 T1 HR P20201617T1 HR P20201617T T HRP20201617T T HR P20201617TT HR P20201617 T HRP20201617 T HR P20201617T HR P20201617 T1 HRP20201617 T1 HR P20201617T1
- Authority
- HR
- Croatia
- Prior art keywords
- stem cells
- epithelial stem
- organoid
- cultivation
- medium
- Prior art date
Links
- 210000000130 stem cell Anatomy 0.000 title claims 17
- 210000002220 organoid Anatomy 0.000 title claims 14
- 239000001963 growth medium Substances 0.000 title claims 2
- 238000000034 method Methods 0.000 claims 11
- 239000012634 fragment Substances 0.000 claims 7
- 239000002609 medium Substances 0.000 claims 7
- 210000001519 tissue Anatomy 0.000 claims 7
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 claims 5
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 claims 5
- 210000002744 extracellular matrix Anatomy 0.000 claims 5
- 108050007372 Fibroblast Growth Factor Proteins 0.000 claims 4
- 102000018233 Fibroblast Growth Factor Human genes 0.000 claims 4
- 102000003972 Fibroblast growth factor 7 Human genes 0.000 claims 4
- 108090000385 Fibroblast growth factor 7 Proteins 0.000 claims 4
- FABQUVYDAXWUQP-UHFFFAOYSA-N N4-(1,3-benzodioxol-5-ylmethyl)-6-(3-methoxyphenyl)pyrimidine-2,4-diamine Chemical compound COC1=CC=CC(C=2N=C(N)N=C(NCC=3C=C4OCOC4=CC=3)C=2)=C1 FABQUVYDAXWUQP-UHFFFAOYSA-N 0.000 claims 4
- 229940126864 fibroblast growth factor Drugs 0.000 claims 4
- 230000002496 gastric effect Effects 0.000 claims 4
- VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 claims 4
- 101800003838 Epidermal growth factor Proteins 0.000 claims 3
- 102400001368 Epidermal growth factor Human genes 0.000 claims 3
- 101000825954 Homo sapiens R-spondin-1 Proteins 0.000 claims 3
- PWKSKIMOESPYIA-BYPYZUCNSA-N L-N-acetyl-Cysteine Chemical compound CC(=O)N[C@@H](CS)C(O)=O PWKSKIMOESPYIA-BYPYZUCNSA-N 0.000 claims 3
- 102100022762 R-spondin-1 Human genes 0.000 claims 3
- 229960004308 acetylcysteine Drugs 0.000 claims 3
- 229940116977 epidermal growth factor Drugs 0.000 claims 3
- 102000045246 noggin Human genes 0.000 claims 3
- 108700007229 noggin Proteins 0.000 claims 3
- NMWKYTGJWUAZPZ-WWHBDHEGSA-N (4S)-4-[[(4R,7S,10S,16S,19S,25S,28S,31R)-31-[[(2S)-2-[[(1R,6R,9S,12S,18S,21S,24S,27S,30S,33S,36S,39S,42R,47R,53S,56S,59S,62S,65S,68S,71S,76S,79S,85S)-47-[[(2S)-2-[[(2S)-4-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-amino-3-methylbutanoyl]amino]-3-methylbutanoyl]amino]-3-hydroxypropanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-3-phenylpropanoyl]amino]-4-oxobutanoyl]amino]-3-carboxypropanoyl]amino]-18-(4-aminobutyl)-27,68-bis(3-amino-3-oxopropyl)-36,71,76-tribenzyl-39-(3-carbamimidamidopropyl)-24-(2-carboxyethyl)-21,56-bis(carboxymethyl)-65,85-bis[(1R)-1-hydroxyethyl]-59-(hydroxymethyl)-62,79-bis(1H-imidazol-4-ylmethyl)-9-methyl-33-(2-methylpropyl)-8,11,17,20,23,26,29,32,35,38,41,48,54,57,60,63,66,69,72,74,77,80,83,86-tetracosaoxo-30-propan-2-yl-3,4,44,45-tetrathia-7,10,16,19,22,25,28,31,34,37,40,49,55,58,61,64,67,70,73,75,78,81,84,87-tetracosazatetracyclo[40.31.14.012,16.049,53]heptaoctacontane-6-carbonyl]amino]-3-methylbutanoyl]amino]-7-(3-carbamimidamidopropyl)-25-(hydroxymethyl)-19-[(4-hydroxyphenyl)methyl]-28-(1H-imidazol-4-ylmethyl)-10-methyl-6,9,12,15,18,21,24,27,30-nonaoxo-16-propan-2-yl-1,2-dithia-5,8,11,14,17,20,23,26,29-nonazacyclodotriacontane-4-carbonyl]amino]-5-[[(2S)-1-[[(2S)-1-[[(2S)-3-carboxy-1-[[(2S)-1-[[(2S)-1-[[(1S)-1-carboxyethyl]amino]-4-methyl-1-oxopentan-2-yl]amino]-4-methyl-1-oxopentan-2-yl]amino]-1-oxopropan-2-yl]amino]-1-oxopropan-2-yl]amino]-3-(1H-imidazol-4-yl)-1-oxopropan-2-yl]amino]-5-oxopentanoic acid Chemical compound CC(C)C[C@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](C)NC(=O)[C@H](Cc1c[nH]cn1)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H]1CSSC[C@H](NC(=O)[C@@H](NC(=O)[C@@H]2CSSC[C@@H]3NC(=O)[C@H](Cc4ccccc4)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@H](Cc4c[nH]cn4)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H]4CCCN4C(=O)[C@H](CSSC[C@H](NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](Cc4c[nH]cn4)NC(=O)[C@H](Cc4ccccc4)NC3=O)[C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](Cc3ccccc3)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCCN)C(=O)N3CCC[C@H]3C(=O)N[C@@H](C)C(=O)N2)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](Cc2ccccc2)NC(=O)[C@H](Cc2c[nH]cn2)NC(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)[C@@H](N)C(C)C)C(C)C)[C@@H](C)O)C(C)C)C(=O)N[C@@H](Cc2c[nH]cn2)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](Cc2ccc(O)cc2)C(=O)N[C@@H](C(C)C)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N1)C(=O)N[C@@H](C)C(O)=O NMWKYTGJWUAZPZ-WWHBDHEGSA-N 0.000 claims 1
- 102000008186 Collagen Human genes 0.000 claims 1
- 108010035532 Collagen Proteins 0.000 claims 1
- 102100028412 Fibroblast growth factor 10 Human genes 0.000 claims 1
- 102400000921 Gastrin Human genes 0.000 claims 1
- 108010052343 Gastrins Proteins 0.000 claims 1
- 101000917237 Homo sapiens Fibroblast growth factor 10 Proteins 0.000 claims 1
- 101000825949 Homo sapiens R-spondin-2 Proteins 0.000 claims 1
- 101000825960 Homo sapiens R-spondin-3 Proteins 0.000 claims 1
- 101000825962 Homo sapiens R-spondin-4 Proteins 0.000 claims 1
- 102100022763 R-spondin-2 Human genes 0.000 claims 1
- 102100022766 R-spondin-3 Human genes 0.000 claims 1
- 102100022759 R-spondin-4 Human genes 0.000 claims 1
- 101800004564 Transforming growth factor alpha Proteins 0.000 claims 1
- 102400001320 Transforming growth factor alpha Human genes 0.000 claims 1
- DFPAKSUCGFBDDF-ZQBYOMGUSA-N [14c]-nicotinamide Chemical compound N[14C](=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-ZQBYOMGUSA-N 0.000 claims 1
- 239000000556 agonist Substances 0.000 claims 1
- 210000004102 animal cell Anatomy 0.000 claims 1
- 210000004556 brain Anatomy 0.000 claims 1
- 239000006143 cell culture medium Substances 0.000 claims 1
- AOXOCDRNSPFDPE-UKEONUMOSA-N chembl413654 Chemical compound C([C@H](C(=O)NCC(=O)N[C@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@H](CCSC)C(=O)N[C@H](CC(O)=O)C(=O)N[C@H](CC=1C=CC=CC=1)C(N)=O)NC(=O)[C@@H](C)NC(=O)[C@@H](CCC(O)=O)NC(=O)[C@@H](CCC(O)=O)NC(=O)[C@@H](CCC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H]1N(CCC1)C(=O)CNC(=O)[C@@H](N)CCC(O)=O)C1=CC=C(O)C=C1 AOXOCDRNSPFDPE-UKEONUMOSA-N 0.000 claims 1
- 229920001436 collagen Polymers 0.000 claims 1
- 239000003814 drug Substances 0.000 claims 1
- 238000007876 drug discovery Methods 0.000 claims 1
- 210000002919 epithelial cell Anatomy 0.000 claims 1
- NGOGFTYYXHNFQH-UHFFFAOYSA-N fasudil Chemical compound C=1C=CC2=CN=CC=C2C=1S(=O)(=O)N1CCCNCC1 NGOGFTYYXHNFQH-UHFFFAOYSA-N 0.000 claims 1
- 229960002435 fasudil Drugs 0.000 claims 1
- 239000003102 growth factor Substances 0.000 claims 1
- 210000005260 human cell Anatomy 0.000 claims 1
- 238000000338 in vitro Methods 0.000 claims 1
- 238000011534 incubation Methods 0.000 claims 1
- 239000003112 inhibitor Substances 0.000 claims 1
- 230000002297 mitogenic effect Effects 0.000 claims 1
- 210000000440 neutrophil Anatomy 0.000 claims 1
- 230000001172 regenerating effect Effects 0.000 claims 1
- 102000000568 rho-Associated Kinases Human genes 0.000 claims 1
- 108010041788 rho-Associated Kinases Proteins 0.000 claims 1
- 239000011435 rock Substances 0.000 claims 1
- 238000012216 screening Methods 0.000 claims 1
- 231100000820 toxicity test Toxicity 0.000 claims 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Materials For Medical Uses (AREA)
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US14962209P | 2009-02-03 | 2009-02-03 | |
EP09151970 | 2009-02-03 | ||
EP09171831 | 2009-09-30 | ||
EP16151949.1A EP3061808B1 (en) | 2009-02-03 | 2010-02-03 | Culture medium for epithelial stem cells and organoids comprising said stem cells |
Publications (1)
Publication Number | Publication Date |
---|---|
HRP20201617T1 true HRP20201617T1 (hr) | 2020-12-11 |
Family
ID=68577677
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
HRP20201617TT HRP20201617T1 (hr) | 2009-02-03 | 2020-10-08 | Medij kulture za epitelijalne matične stanice i organoidi koji se sastoje iz spomenutih matičnih stanica |
Country Status (4)
Country | Link |
---|---|
ES (1) | ES2825713T3 (es) |
HR (1) | HRP20201617T1 (es) |
HU (1) | HUE050984T2 (es) |
MX (1) | MX2019006565A (es) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114763534B (zh) * | 2021-01-13 | 2024-04-26 | 合肥中科普瑞昇生物医药科技有限公司 | 原代胃肠间质瘤细胞培养基、培养方法及其应用 |
-
2010
- 2010-02-03 ES ES16151949T patent/ES2825713T3/es active Active
- 2010-02-03 HU HUE16151949A patent/HUE050984T2/hu unknown
-
2011
- 2011-07-29 MX MX2019006565A patent/MX2019006565A/es unknown
-
2020
- 2020-10-08 HR HRP20201617TT patent/HRP20201617T1/hr unknown
Also Published As
Publication number | Publication date |
---|---|
MX2019006565A (es) | 2019-08-22 |
ES2825713T3 (es) | 2021-05-17 |
HUE050984T2 (hu) | 2021-01-28 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
HRP20160791T1 (hr) | Kultivirani medij za epitelne matične stanice i organoidi koji sadrže navedene matične stanice | |
HRP20171760T1 (hr) | Medij za kultiviranje matičnih stanica | |
JP2012254081A5 (es) | ||
CN103620024B (zh) | 用于使多能干细胞分化为血管床细胞的方法 | |
EP3031908B1 (en) | Method for producing dopaminergic neurons | |
Daoud et al. | The effect of extracellular matrix components on the preservation of human islet function in vitro | |
Kimlin et al. | 3D in vitro tissue models and their potential for drug screening | |
RU2013108820A (ru) | Органоид печени, варианты его применения и способ культивирования для его получения | |
CN108367099A (zh) | 来自多能干细胞的人皮肤类器官的衍生 | |
CN111684058B (zh) | 从多能干细胞向肠上皮细胞的分化诱导方法 | |
EP3430132A1 (en) | Generation of midbrain-specific organoids from human pluripotent stem cells | |
Xu et al. | Encapsulated human hepatocellular carcinoma cells by alginate gel beads as an in vitro metastasis model | |
CN109661460A (zh) | 用于人腹泻症病毒的感染·增殖培养的2d类器官及其使用 | |
KR20130099035A (ko) | 췌호르몬-생성 세포의 제조 방법 | |
MY188836A (en) | Method for manufacturing telencephalon or progenitor tissue thereof | |
CN109072198A (zh) | 产多巴胺神经祖细胞的制备方法 | |
Lonardo et al. | Studying pancreatic cancer stem cell characteristics for developing new treatment strategies | |
Zhao et al. | Essential roles of HDAC1 and 2 in lineage development and genome-wide DNA methylation during mouse preimplantation development | |
RU2015140912A (ru) | Культивирование эпителиальных стволовых клеток в жидких питательных средах | |
CN116981768A (zh) | 将人类体细胞化学重编程为多能细胞 | |
Rodrigues Toste de Carvalho et al. | The in vitro multilineage differentiation and maturation of lung and airway cells from human pluripotent stem cell–derived lung progenitors in 3D | |
HRP20201617T1 (hr) | Medij kulture za epitelijalne matične stanice i organoidi koji se sastoje iz spomenutih matičnih stanica | |
Kinney et al. | Mesenchymal morphogenesis of embryonic stem cells dynamically modulates the biophysical microtissue niche | |
CN111004771A (zh) | 建立体外模拟肝病模型的方法及其专用三维培养基 | |
US20210222123A1 (en) | In vitro expansion of dopaminergic subtype neuronal progenitors derived from pluripotent stem cells |