GB2628547A - Probiotic and postbiotic compositions, products and uses thereof - Google Patents
Probiotic and postbiotic compositions, products and uses thereof Download PDFInfo
- Publication number
- GB2628547A GB2628547A GB2304447.2A GB202304447A GB2628547A GB 2628547 A GB2628547 A GB 2628547A GB 202304447 A GB202304447 A GB 202304447A GB 2628547 A GB2628547 A GB 2628547A
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- GB
- United Kingdom
- Prior art keywords
- clostridium
- composition
- postbiotic
- subject
- probiotic
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Abstract
A probiotic composition is disclosed for improving the gut microbiome and/or gut health of a subject comprising at least one Clostridium species selected from Clostridium perfringens, Clostridium baratii and Clostridium tertium. Also disclosed are postbiotic compositions from the aforementioned Clostridium species for the same purpose. Products comprising the said compositions and methods of using the said compositions are also disclosed.
Description
Probiotic and postbiotic compositions, products and uses thereof The present invention provides a probiotic composition for improving the gut microbiome and/or gut health of a subject comprising at least one Clostridium species selected from Clostridium perfringens, Clostridium baratii and Clostridium tertium. It also provides postbiotic compositions from these Clostridium species for the same purpose. Products comprising the compositions and methods of using the compositions are also provided herein.
Background
The bacteria and other microorganisms that colonise the gut, termed the gut microbiome, play important roles in health and disease. This is especially true during the first year of life, where the gut microbiome contributes to education and training of the immune system, which can have implications for long term health.
Receipt of breast milk is one of the most important modulators of the infant gut microbiome, due in part to the direct provision of prebiotics. Prebiotics are non-digestible substrates that promote the growth of beneficial bacteria, termed probiotics, and an example of prebiotics in breast milk is human milk oligosaccharides (HMOs). HMOs are abundant unconjugated sugars that reach the infant gut intact where they are consumed by selected probiotic bacteria.
Most work to date has focused on species within the Bifidobacterium genus, due in part to the well described genetic capability of Bifidobacterium species to use HMOs. One proposed mechanism involves selected Bifidobacterium species consuming HMOs as an energy source and releasing short chain fatty acids (SCFAs) into the gut lumen. These SCFAs may promote health through a myriad of ways including providing an important energy source for epithelial cells, improving barrier integrity, stimulating goblet cells to produce mucus, anti-inflammatory properties, and protecting against infection.
Unlike infants born at term, preterm infants born under 32 weeks gestation typically spend the initial weeks or months of life in the neonatal intensive care unit (N ICU). Compared to term infants, the preterm infant gut microbiome is less diverse, less stable, contains lower abundance of potentially probiotic bacteria, and a higher abundance of pathobiont bacteria.
This altered gut microbiome has been associated with poorer health and an increased risk to several life-threatening diseases. Probiotics that contain Bifidobacterium species that can utilise HMOs have been used in many NICUs worldwide and overall meta-analyses suggests they can reduce disease risk, primarily relating to late onset sepsis (LOS) and necrotising enterocolitis (NEC). However, it would be desirable to create a probiotic formulation that is more tailored to preterm infants to help improve growth and reduce disease risk. Such a probiotic might also have utility for improving health across the life course.
There is a need for novel compositions that improve the gut microbiome and reduces disease risk, particularly in respect of diseases exacerbated by probiotic imbalance.
Brief summary of the disclosure
The invention is based on the surprising finding that several bacterial species of the Clostridium genus utilise HMOs and therefore represent beneficial probiotics for improving the gut microbiome and/or gut health of a subject. The inventors have surprisingly discovered that Clostridium perfringens, Clostridium baratii and Clostridium tedium utilise a number of HMOs when in co-culture, including at least one of: LNnT, LNT, 6SL, 2FL, DLSNT and LNFP_I. When grown on HMOs, HMO-utilising bacteria produce short chain fatty acids, convert aromatic amino acids into aromatic lactic acids (for example indolelactic acid (ILA)) and release other functionally important compounds that have significant beneficial immunomodulatory roles in the health of a subject. Interestingly, ILA has been shown to limit T cell activation and reduce a pro-inflammatory response by inducing galectin-1 in TH2 and TH17 cells during helper T cell polarisation. Accordingly, consumption of compositions that comprise one or more of these Clostridium species will therefore provide health benefits to a subject.
The inventors have also surprisingly shown that Clostridium perfringens is an abundant and prevalent species present in the gut in early life and is present at higher levels in healthy infants compared to infants diagnosed with necrotising enterocolitis (NEC) during the critical window of NEC risk (the first 10 to 40 days of life). Furthermore, the inventors have demonstrated that Clostridium perfringens is more abundant in the gut of healthy infants compared to infants before they were diagnosed with NEC, but not after recovery from NEC.
This suggests that Clostridium perfringens plays an important role in protecting against NEC and suggests that increased levels of Clostridium perfringens in the gut would be beneficial and protective against disease.
The data provided herein indicates that a probiotic composition comprising one or more bacterial species of the Clostridium genus would improve the gut microbiome in a subject.
Advantageously, all of Clostridium perfringens, Clostridium baratii and Clostridium tedium have been shown to utilise at least one HMO, including LNnT, LNT, 6SL, 2FL, DSLNT and/or LNFP_I, thus sharing a common metabolic pathway and production of, for example, short chain fatty acids. The inventors have also shown that Clostridium perfringens is more abundant in the gut of healthy infants compared to infants before they were diagnosed with NEC, which suggests that such a protective effect would be present for other diseases affecting, for example, the gastrointestinal tract. Notwithstanding the role of Clostridium perfringens in disease outcome for NEC, other therapeutic outcomes are dependent on probiotic levels and as such, it is also reasonable that one or more bacterial species of the Clostridium genus including Clostridium perfringens, Clostridium baratii and Clostridium tedium would be therapeutically beneficial in preventing or treating any disorder that is exacerbated by probiotic imbalance, such as a gastrointestinal disorder, allergy, nappy rash, and colic, or a symptom thereof. By improving the gut microbiome and/or gut health in a subject, Clostridium perfringens, Clostridium baratii and Clostridium tedium can advantageously reduce pathogen overgrowth in the gut, promote gut development, promote gut maturation, promote immunity, decrease gut inflammation, and/or promote enteral feeding tolerance in the subject.
Accordingly, in one aspect the invention provides a probiotic composition for improving the gut microbiome and/or gut health of a subject, the composition comprising at least one Clostridium species selected from the group consisting of: Clostridium perfringens, Clostridium baratii and Clostridium tedium.
Suitably, the composition may comprise at least two Clostridium species selected from the group consisting of: Clostridium perfringens, Clostridium baratii and Clostridium tedium.
Suitably, the composition may comprise at least 0.5 x 109 CFU Clostridium species per dose.
Suitably, the composition may further comprise at least one prebiotic.
Suitably, the prebiotic may be a mammalian milk oligosaccharide (MMO), a precursor and/or a derivative thereof.
Suitably, the MMO may be a human milk oligosaccharide (HMO) a precursor and/or a derivative thereof.
Suitably, the HMO may be selected from the group consisting of: lacto-N-tetraose (LNT), lacto-N-neotetraose (LNnT), disialyllacto-N-tetraose (DSLNT), lacto-N-fucopentaose I (LN FPI), 6'-sialyllactose (6SL) and 2-fucosyllactose (2FL); or a combination thereof; optionally wherein the HMO is LNnT and/or 6SL.
The invention also provides a postbiotic composition for improving the gut health of a subject, the composition comprising: (a) at least one inanimate Clostridium species selected from the group consisting of: Clostridium perfringens, Clostridium baratii and Clostridium tedium; and/or (b) metabolites from at least one Clostridium species selected from the group consisting of: Clostridium perfringens, Clostridium baratii and Clostridium tedium.
Suitably, the postbiotic composition may comprise: (a) at least two inanimate Clostridium species selected from the group consisting of: Clostridium perfringens, Clostridium baratii and Clostridium tedium; and/or (b) metabolites from at least two Clostridium species selected from the group consisting of: Clostridium perfringens, Clostridium baratii and Clostridium tedium.
Suitably, the probiotic composition or postbiotic composition may be in the form of a liquid medium, gel medium, powder, dissolvable medium, chewable medium, or any other ingestible 10 medium.
Suitably, the probiotic composition or postbiotic composition may be a milk product.
Suitably, the probiotic composition or postbiotic composition may be an infant formula, a starter infant formula, a follow-on or follow-up infant formula, a baby food, or an infant cereal composition.
Suitably, the probiotic composition or postbiotic composition may be a powder, a liquid or a solid.
The invention also provides a probiotic composition or postbiotic composition of the invention for use in improving the gut microbiome and/or the gut health of a subject.
Suitably, the subject may be an infant.
Suitably, the infant may be a neonate, a preterm infant, an infant small for gestational age, or an infant born by C-section.
Suitably, the infant may be hospitalised and/or is undergoing antibiotic treatment.
Suitably, the composition reduces pathogen overgrowth in the gut, promotes gut development, promotes gut maturation, promotes immunity, decreases gut inflammation, and/or promotes enteral feeding tolerance in the subject.
The invention also provides a probiotic composition or postbiotic composition of the invention for use in preventing or treating a gastrointestinal disorder, an allergy, nappy rash, colic, or a symptom thereof, in a subject.
The invention also provides a method of treating or preventing a gastrointestinal disorder, an allergy, nappy rash, colic, or a symptom thereof in a subject, comprising administering a therapeutically effective amount of the probiotic composition or the postbiotic composition of the invention to said subject.
Suitably, the gastrointestinal disorder may affect the integrity of the intestinal lining.
Suitably, the gastrointestinal disorder may be selected from the group consisting of: intestinal inflammation, irritable bowel syndrome, colitis, necrotising enterocolitis, late onset sepsis, focal intestinal perforation, and inflammatory bowel disease.
Suitably, the gastrointestinal disorder may be necrotising enterocolitis. Suitably, the subject may be an infant.
Suitably, the infant may be a neonate, a preterm infant, an infant small for gestational age, or an infant born by C-section.
Suitably, the infant may be hospitalised and/or is undergoing antibiotic treatment.
The invention also provides the use of Clostridium perfringens, Clostridium baratii and/or Clostridium tedium as a probiotic or postbiotic.
The invention also provides Clostridium perfringens, Clostridium baratii and/or Clostridium tedium, or a Clostridium postbiotic thereof, for use in improving the gut microbiome and/or gut health in a subject.
Various aspects of the invention are described in further detail below.
Brief description of the Figures
Embodiments of the invention are further described hereinafter with reference to the accompanying drawings, in which: Figure 1 shows that several species of Clostridium and Bifidobacterium can use a range of HMOs. White squares containing "NA" in the DSLNT row indicate these co-cultures were not performed due to limited availability of DSLNT.
Figure 2 shows Clostridium perfringens is the most abundant Clostridium species in early life and is higher in healthy infants compared to infants diagnosed with necrotising enterocolitis during the critical window of NEC risk. DOL: day of life.
Figure 3 shows Bifidobacterium is similar between healthy infants and infants diagnosed with necrotising enterocolitis during the critical window of NEC risk. DOL: day of life.
Figure 4 shows Clostridium perfringens is higher in healthy infants (NoNEC) compared to infants before they were diagnosed with necrotising enterocolitis (PreNEC), but not after recovery from NEC (PostNEC).
Figure 5 shows the postbiotic (i.e., conditioned medium) from the growth of Clostridium perfringens on HMOs was inhibitory to a range of pathobionts (E. coli, E. faecalis, K. oxytoca and K pneumoniae) The patent, scientific and technical literature referred to herein establish knowledge that was available to those skilled in the art at the time of filing. The entire disclosures of the issued patents, published and pending patent applications, and other publications that are cited herein are hereby incorporated by reference to the same extent as if each was specifically and individually indicated to be incorporated by reference. In the case of any inconsistencies, the present disclosure will prevail.
Various aspects of the invention are described in further detail below.
Detailed Description Probiotic compositions
The present invention relates to a probiotic composition for improving the gut microbiome and/or gut health of a subject, the composition comprising at least one Clostridium species selected from the group consisting of: Clostridium perfringens, Clostridium baratii and Clostridium tedium.
The inventors have surprisingly identified that Clostridium species Clostridium perfringens, Clostridium baratii and Clostridium tedium are able to use a wide variety of HMOs as a carbon source. To the inventors' knowledge, this is the first time these species have been identified as probiotics for improving the gut microbiome and/or gut health, particularly in infants such as preterm infants.
The term "probiotic composition" as used herein refers to a composition comprising live bacteria that are beneficial to the overall health of an individual (referred to as a subject herein) that consumes the composition. Probiotic compositions are well known in the art. Probiotic compositions that are described herein impart their beneficial effects in the gut of the subject, and therefore improve the gut microbiome and/or gut health of the subject. Probiotic compositions may also be described as compositions that are utilised as a dietary supplement to alter the digestive tract microbiota of a subject in a beneficial manner without causing disease.
As would be clear to a person of skill in the art, a probiotic composition comprises sufficient quantities of the live bacteria to impart the required beneficial effect on the subject. The amount of bacteria in the probiotic composition may be referred to as a therapeutically effective amount. Appropriate amounts of live bacteria can readily be identified by a person skilled in the art. Further details and examples are provided elsewhere herein.
The probiotic potential of Clostridium species has been identified previously (see for example, Guo et al., 2020 (Journal of animal science and biotechnology); and Cartman, Future Microbiol. (2011), 6(9), 969-971). Although several Clostridium strains are considered to be pathogenic, Clostridium species account for 10-40% of the total bacteria in the human gut and are predominantly commensal in nature.
The probiotic compositions provided herein comprise live bacteria from at least one Clostridium species selected from the group consisting of: Clostridium perfringens, Clostridium baratii, or Clostridium tedium. In one example, the probiotic compositions provided herein may comprise live bacteria from at least two Clostridium species selected from the group consisting of: Clostridium perfringens, Clostridium baratii, or Clostridium tedium. For example, the probiotic composition may comprise live Clostridium perfringens and Clostridium baratii. In another example, the probiotic composition may comprise live Clostridium perfringens and Clostridium tedium. In another example, the probiotic composition may comprise live Clostridium baratii and Clostridium tedium. In another example, the probiotic composition may comprise live Clostridium perfringens, Clostridium baratii and Clostridium tedium.
Clostridium (Clostridiaceae) is a genus of rod-shaped, anaerobic, chemoorganotrophic (ability to ferment), and spore-forming bacteria belonging to the Bacillaceae family of the phylum Firmicutes. Non-limiting examples of Clostridium species include Clostridium perfringens, Clostridium baratii, Clostridium tedium, Clostridium butyricum, Clostridium botulinum, Clostridium sporogenes, Clostridium difficile, Clostridium tetani, and Clostridium ramosum. Species of Clostridium are ubiquitous; naturally colonising the environmental medium (e.g. soil, water, and marine sediment) and the digestive tract of humans and animals. Clostridium species are a well-understood regulator of intestinal homeostasis. Clostridium species are Gram-variable, as they generally stain Gram-positive, however, as the culture ages, an increase in Gram-negative cells is often observable. Methods for performing a Gram stain are well known in the art.
Clostridium species primarily exist in three states: vegetative, spore-forming, and as an endospore. Clostridium species in either the vegetative or spore-forming states are considered "live" herein, as they are able to grow and reproduce. The term 'spore-forming' as used herein is intended to refer to Clostridium species at any stage of the sporulation cycle, except free endospore. The terms "spore(s)" and "endospore(s)" as used herein are interchangeable and refer to a dormant, tough, and non-reproductive free structure produced by the Clostridium species.
Clostridium perfringens is an obligate anaerobe, and is categorised into non-pathogenic and pathogenic strains, with the latter comprising seven types: A, B, C, D, E, F, and G. Nonpathogenic Clostridium perfringens strains comprise Clostridium perfringens strain SM101 and type A, and these are capable of producing secondary bile acids. Pathogenic Clostridium perfringens strains are frequently responsible for food poisoning and a large proportion gas gangrene cases.
The probiotic compositions described herein that comprise live Clostridium perfringens are beneficial to the overall health of an individual (referred to as a subject herein) that consumes the composition. The probiotic compositions described herein that comprise live Clostridium perfringens impart their beneficial effects in the gut of the subject, and therefore improve the gut microbiome of the subject. The probiotic compositions described herein that comprise live Clostridium perfringens may be utilised as a dietary supplement to alter the digestive tract microbiota of a subject in a beneficial manner without causing disease. In one example, the Clostridium perfringens is therefore a non-pathogenic strain, such as Clostridium perfringens strain SM 101 and type A. Several Clostridium perfringens strains have been characterised and the presence or absence of toxin expression and/or antimicrobial resistance (AMR) in these strains has been studied previously (see for example, Kiu et al., Dissemination and pathogenesis of toxigenic Clostridium perfringens strains linked to neonatal intensive care units and Necrotising Enterocolitis; 2021 bioRxiv preprint doi: https://doi.org/10.1101/2021.08.03.454877). A person of skill in the art can therefore readily identify non-pathogenic strains of Clostridium perfringens.
In some examples, the Clostridium perfringens provided herein is pfoA-negative. In other words, they do not express the pore-forming toxin perfringolysin 0; PFO, which has been heavily associated with the pathogenesis of myonecrosis (gas gangrene).
In some examples, the Clostridium perfringens provided herein is a cpb2-negative. In other words, they do not express beta2-toxin, CPB2, which has been associated with virulence.
In some examples, the Clostridium perfringens provided herein does not comprise the plasmid pCW3 and/or pCP13, which have long been recognised as key mobile genetic elements for transfer of virulence genes, including toxins.
In some examples, the Clostridium perfringens provided herein is negative for one or more of the following toxin-associated genes: cpe, iap, ibp becA, becB and/or edpA.
The toxin genes pfoA, cpe, iap, ibp becA, becB and/or edpA are well known. For more details see for example Kiu et al., 2021, referenced above.
In some examples, the Clostridium perfringens provided herein is negative for one or more antimicrobial resistance genes selected from the group consisting of: cfrC, ET-Tu, gyrA, gryB, rpoC, and TetA. For example it may be negative for each of them (i.e. negative for all of the following): cfrC, ET-Tu, gyrA, gryB, rpoC, and TetA In some examples, the Clostridium perfringens provided herein is negative for rpoB.
Clostridium baratii is an obligate anaerobe, and is rarely associated with human disease, however, when it is, it will usually occur after eating food contaminated with vegetative Clostridium baratii or its spores. Clostridium baratii can produce toxins which cause botulism, and is a rare cause of infant botulism.
The probiotic compositions described herein that comprise live Clostridium baratii are beneficial to the overall health of an individual (referred to as a subject herein) that consumes the composition. The probiotic compositions described herein that comprise live Clostridium baratii impart their beneficial effects in the gut of the subject, and therefore improve the gut microbiome of the subject. The probiotic compositions described herein that comprise live Clostridium baratii may be utilised as a dietary supplement to alter the digestive tract microbiota of a subject in a beneficial manner without causing disease. In one example, the Clostridium baratii is therefore a non-pathogenic strain.
Several Clostridium baratii strains have been characterised and the presence or absence of toxin expression and/or antimicrobial resistance (AMR) of these strains has been studied previously -see for example Silva-Andrade et al., Microorganisms. 2022 Reb; 10(2): 213. A person of skill in the art can therefore readily identify non-pathogenic strains of Clostridium baratii.
In some examples, the Clostridium baratii provided herein is negative for one or more toxin genes selected from the group consisting of: boNT, colA, NagH, Nagl, NagJ, NagK, NagL, nanH, nanl, nanJ, tetX, and pfoA. For example it may be negative for each of them (i.e. negative for all of the following): boNT, colA, NagH, Nagl, NagJ, NagK, NagL, nanH, nanl, nanJ, tetX, and pfoA. In some examples, the Clostridium baratii provided herein is negative for plc (alpha toxin phospholipase C).
NanH, nanl, and nanJ genes express sialidases. pfoA expresses theta-toxin/perfringolysin 0.
NagH, Nagl, NagJ, nagK and NagL genes express mu-toxins, and colA expresses microbial collagenase. BoNT genes express botulinum toxins and tetX genes express tetanus toxin.
For more information see for example Silva-Andrade et al., 2022 Feb: 10(2): In some examples, the Clostridium baratii provided herein is negative for one or more antimicrobial resistance genes selected from the group consisting of: cfrC, ET-Tu, gyrA, gryB, rpoC, and TetA. For example it may be negative for each of them (i.e. negative for all of the following): cfrC, ET-Tu, gyrA, gryB, rpoC, and TetA. In some examples, the Clostridium baratii provided herein is negative for rpoB.
The antimicrobial genes cfrC, ET-Tu, gyrA, gryB, rpoC, TetA and rpoB are well characterised. For more details see for example Silva-Andrade et al., Microorganisms. 2022 Feb: 10(2): 213.
Clostridium tedium is both anaerobic and aerotolerant. Although the normal habitat of Clostridium tedium is the soil, it is commonly found as a commensal colonising the digestive tract of humans and animals. Although rarely pathogenic, it is thought to be associated with enterocolitis in preterm neonates.
The probiotic compositions described herein that comprise live Clostridium tedium are beneficial to the overall health of an individual (referred to as a subject herein) that consumes the composition. The probiotic compositions described herein that comprise live Clostridium tedium impart their beneficial effects in the gut of the subject, and therefore improve the gut microbiome of the subject. The probiotic compositions described herein that comprise live Clostridium tedium may be utilised as a dietary supplement to alter the digestive tract microbiota of a subject in a beneficial manner without causing disease. In one example, the Clostridium tedium is therefore a non-pathogenic strain.
Several Clostridium tedium strains have been characterised and the presence or absence of toxin expression and/or antimicrobial resistance (AMR) of these strains has been studied previously. A person of skill in the art can therefore readily identify non-pathogenic strains of Clostridium tedium.
In some examples, the Clostridium tedium provided herein is negative for one or more antimicrobial resistance genes selected from the group consisting of cfrC, ET-Tu, gyrA, gryB, rpoC, and TetA. For example it may be negative for each of them (i.e. negative for all of the following): cfrC, ET-Tu, gyrA, gryB, rpoC, and TetA. In some examples, the Clostridium tedium provided herein is negative for rpoB.
Probiotic bacteria may be combined with a prebiotic. They can be combined into a single composition that comprises both the probiotic bacteria and at least one prebiotic such that they are consumed simultaneously by the subject (in other words, a probiotic composition provided herein may further comprise a prebiotic as well). Alternatively, they may be in separate (distinct) compositions, whereby the probiotic composition of the invention and a prebiotic composition are consumed separately (simultaneously or consecutively) by the subject.
Accordingly, in one example, at least one prebiotic is present within the probiotic composition. In another example, at least one prebiotic is provided as a separate prebiotic composition (separate from the probiotic composition) and is administered to the subject either prior, after, or during administration of the probiotic composition. Examples of such prebiotic compositions are described elsewhere herein.
A probiotic composition is therefore provided, wherein the probiotic bacteria are combined with at least one prebiotic within the same composition. Such compositions may also be referred to herein as a "synbiotic" composition.
A prebiotic composition is also provided, which lacks the probiotic bacteria described herein (i.e. lacks Clostridium perfringens, Clostridium baratii and Clostridium tedium), wherein the prebiotic composition is for separate (simultaneous or consecutive) administration to the same subject as the probiotic composition of the invention. Details of the prebiotic composition are provided elsewhere herein.
The term "prebiotic" as used herein refers to a component that promotes the growth of probiotic bacteria. In certain instances, prebiotic may refer to food ingredients or bacterially produced substances that are not readily digestible by endogenous host enzymes (in other words, the prebiotic is not digestible by the subject that consumes the composition of the invention, but rather by the probiotic bacteria that may colonise its gut). Prebiotics may originate from several sources, such as artichokes, asparagus, bananas, berries, tomatoes, garlic, onions, legumes, green vegetables, wholegrain cereals, and milk, such as breast milk or cows' milk. Prebiotics may for example be MMOs (mammalian milk oligosaccharides) such as HMOs (human milk oligosaccharides). In certain instances, prebiotics may be synthetic in origin or manufactured artificially.
As chemoorganotrophic bacteria, Clostridium species are able to ferment several nutrients including, but not limited to, protein, carbohydrate, organic acid, and non-digestible substrates (such as oligosaccharides), to generate short-chain fatty acids (SCFAs) such as propionic acid, butyric acid, acetic acid, and solvents such as acetone and butanol.
The Clostridium species Clostridium perfringens, Clostridium baratii and Clostridium tedium are able to use a wide variety of prebiotics, in particular MMOs such as HMOs. This makes them to useful probiotics that can improve the gut microbiome and/or gut health, particularly in infants that readily consume milk (e.g. in the form of infant formula, breast milk etc) that readily has MMOs, such as HMOs therein. This leads to the production of short chain fatty acids, conversion of aromatic amino acids into aromatic lactic acids, for example indolelactic acid (ILA) and the release of other functionally important compounds that have significant immunomodulatory roles in the health of a subject.
The probiotic composition of the invention may therefore comprise at least one prebiotic. In one example it comprises at least two, at least three, at least four, at least five, at least six, at least seven, at least eight, at least nine, at least ten, at least eleven, at least twelve, at least thirteen, at least fourteen etc prebiotics.
In one example, the prebiotic is a mammalian milk oligosaccharide (MMO), a precursor and/or a derivative thereof.
MMO, as used herein, refers to indigestible glycans, sometimes referred to as "dietary fiber", or carbohydrate polymers found in mammalian milk which are not metabolized by any combination of mammalian digestive enzymes. Mammalian milk contains a significant quantity of MMO that is not usable directly as an energy source for the milk-fed mammal but may be usable by many of the microorganisms in the gut of that mammal. MMOs can be found as free oligosaccharides (3 sugar units or longer, e.g., 3-20 sugar residues) or they may be conjugated to proteins or lipids. Oligosaccharides having the chemical structure of the indigestible oligosaccharides found in any mammalian milk are called "MMO" or "mammalian milk oligosaccharides" herein, whether or not they are actually sourced from mammalian milk. The MMO can include one or more of fucosyllactose, lacto-N-fucopentose, lactodifucotetrose, sialyllactose, disialyllacto-N-tetraose, 2'-fucosyllactose, 3'-sialyllactoseamin, 3'-fucosyllactose, 3'-sialyl-3-fucosyllactose, 3'-sialyllactose, 6'-sialyllactosamine, 6'-sialyllactose, difucosyllactose, lacto-N-fucosylpentose I, lacto-N-fucosylpentose II, lacto-N-fucosylpentose III, lacto-N-fucosylpentose V, sialyllacto-N-tetraose, or derivatives thereof. See, e.g., U.S. Pat. Nos. 8,197,872, 8,425,930, and 9,200,091, the disclosures of which are incorporated herein by reference in their entirety.
Several MMOs are well known in the art, including that from human milk (HMO), bovine milk (BMO), ovine milk (OMO), equine milk (EMO) or caprine milk (CMO). The oligosaccharides can be obtained from a process that involves cheese or yogurt production and can be from whey sources such as, but not limited to, the whey permeate, or a processed whey permeate, where the processing steps may include, but are not limited to, removal of lactose, removal of minerals, removal of peptides, and removal of monosaccharides, but which in any case, results in the concentration of the MMO to levels that are greater than 20%, greater than 30%, greater than 40%, greater than 50%, greater than 60%, greater than 70%, or greater than 80% of the total dry matter of the product.
In one example, the probiotic composition of the invention comprises at least one MMO. In other example, a prebiotic composition comprising at least one MMO is administered to the subject as a separate (distinct) composition to the probiotic composition of the invention, whereby the probiotic composition of the invention and a prebiotic composition are consumed separately (simultaneously or consecutively) by the subject. In this context, the prebiotic composition comprising at least one MMO may be a milk selected from the group consisting of human milk (HMO), bovine milk (BMO), ovine milk (OMO), equine milk (EMO) or caprine milk (CMO).
In one example, the prebiotic is a human milk oligosaccharide (HMO), a precursor and/or a derivative thereof.
HMOs are a complex mixture of more than 200 non-digestible and non-nutritional carbohydrate polymers, which are not metabolised by any combination of human digestive enzymes in the digestive tract. These HMOs occur naturally in human breast milk only. As they are non-digestible, the subject is unable to utilise them as an energy source. However, HMOs are a bonafide energy source for several probiotic members of the gut microbiota in the subject. After lactose and lipids, HMOs are the third most abundant component of breast milk. Human breast milk contains three major HMO types: fucosylated HMOs, sialylated HMOs, and nonfucosylated neutral HMOs, which typically make up 35-50%, 12-14%, and 4255% of the total HMO content of milk, respectively. HMOs are made of five basic monosaccharides: D-glucose, D-galactose, N-acetylglucosamine, L-fucose, and NAcetylneuraminic acid (sialic acid). The majority of HMOs are based on a lactose molecule at the reducing end, and branching can be linear or branched through beta (1-3) or beta (1-6) bonds. Non-limiting examples of exemplary HMOs include lacto-N-tetraose (LNT), lacto-Nneotetraose (LNnT), disialyllacto-N-tetraose (DSLNT), lacto-N-fucopentaose I (LNFPI), 6'-sialyllactose (6SL), and 2-fucosyllactose (2FL). These HMOs are well known and are well characterised in the art. HMOs are virtually absent in non-human milk and in unsupplemented infant formula.
The HMO used for promoting colonisation and/or growth of probiotic bacteria present in the probiotic compositions described herein can include fucosylated HMOs, precursors, or derivatives thereof. Accordingly, the probiotic compositions provided herein may comprise, or may be administered in combination with, a fucosylated HMO, precursor or derivative thereof (prebiotic). Non-limiting examples of fucosylated HMOs include 2FL, LNFPI, and LDFH I which can be purified from human milk or produced directly from chemical synthesis. 2FL is the most abundant HMO, constituting nearly 30% of the total HMOs in human breast milk.
The HMO used for promoting colonisation and/or growth of the probiotic bacteria present in the probiotic compositions described herein can include sialylated HMOs, precursors or derivatives thereof. Accordingly, the probiotic compositions provided herein may comprise, or may be administered in combination with, a sialylated HMO, precursor or derivative thereof (prebiotic). Non-limiting examples of sialylated HMO derivatives include DSLNT, 3SL, 6SL, LSTa, LSTb, LSTc.
The HMO used for promoting colonisation and/or growth of the probiotic bacteria present in the probiotic compositions described herein can include nonfucosylated neutral HMOs, precursors or derivatives thereof. Accordingly, the probiotic compositions provided herein may comprise, or may be administered in combination with, a nonfucosylated neutral HMO, precursor or derivative thereof (prebiotic). Non-limiting examples of nonfucosylated neutral HMOs include LNT, LNnT and LNnH.
In one example, the HMO that is present in the probiotic composition of the invention is selected from the group consisting of: lacto-N-tetraose (LNT), lacto-N-neotetraose (LNnT), disialyllacto-N-tetraose (DSLNT), lacto-N-fucopentaose I (LNFPI), 6'-sialyllactose (6SL) and 2-fucosyllactose (2FL); or a combination thereof.
In one example, the HMO that is present in the probiotic composition of the invention is LNnT and/or 6SL.
In one example, the probiotic composition of the invention comprises at least one HMO. In other example, a prebiotic composition comprising at least one HMO is administered to the subject as a separate (distinct) composition to the probiotic composition of the invention, whereby the probiotic composition of the invention and a prebiotic composition are consumed separately (simultaneously or consecutively) by the subject.
The abundance of specific HMOs in human milk is variable, and may change based on the genetics, diet, physiological state of the mother, pathological state of the mother, and/or psychological state of the mother. The HMO composition received by the subject at a certain time point may reflect that of a healthy mother at the corresponding time point.
In one example, a plurality of HMOs are present within the probiotic composition of the invention. For example, the probiotic composition may comprise at least two, at least three, at least four, at least five, at least six etc HMOs.
The HMOs present in the probiotic composition of the invention may be a mixture of lacto-Ntetraose (LNT), lacto-N-neotetraose (LNnT), disialyllacto-N-tetraose (DSLNT), lacto-Nfucopentaose I (LNFPI), 6'-sialyllactose (6SL), and/or 2-fucosyllactose (2FL), which are all naturally found in human milk.
In one example, the probiotic composition of the invention comprises human milk.
In other example, human milk is administered to the subject as a separate (distinct) composition to the probiotic composition of the invention, whereby the probiotic composition of the invention and a prebiotic composition are consumed separately (simultaneously or consecutively) by the subject.
In a further example, the probiotic composition of the invention comprises at least one HMO precursor. The term "HMO precursor" as used herein refers to mono-, di-and trisaccharides that are the parts of HMOs, that is glucose, galactose, N-acetyl-glucosamine, fucose, sialic acid, lactose, lacto-N-biose (Gaipi-3GIcNAc), N-acetyl-lactosamine (Gaipi-4GIcNAc) and lacto-N-triose (GIcNAcpi-3Gaipi-4G1c). Preferred HMO precursors are selected from the group consisting of fucose, sialic acid, lacto-N-biose, N-acetyl-lactosamine and lacto-N-triose.
In one example, the probiotic composition of the invention comprises at least one HMO precursor. In other example, a prebiotic composition comprising at least one HMO precursor is administered to the subject as a separate (distinct) composition to the probiotic composition of the invention, whereby the probiotic composition of the invention and a prebiotic composition are consumed separately (simultaneously or consecutively) by the subject.
In one example, the probiotic composition of the invention comprises Clostridium perfringens and at least one prebiotic. For example, Clostridium perfringens and LNnT, 6SL, 2FL, DSLNT, LNFP-I and/or LNT.
In one example, the probiotic composition of the invention comprises Clostridium perfringens and LNnT, 6SL, 2FL, DSLNT, and/or LNT.
In one example, the probiotic composition of the invention comprises Clostridium perfringens and LNnT, 6SL, 2FL, and/or DSLNT.
In one example, the probiotic composition of the invention comprises Clostridium perfringens 25 and LNnT, 6SL and/or DSLNT.
In one example, the probiotic composition of the invention comprises Clostridium perfringens and LNnT and/or 6SL.
In one example, the probiotic composition of the invention comprises Clostridium perfringens and LNnT.
In one example, the probiotic composition of the invention comprises Clostridium perfringens and 6SL In one example, the probiotic composition of the invention comprises Clostridium baratii and at least one prebiotic. For example, Clostridium baratii and LNnT, and/or LNT.
In one example, the probiotic composition of the invention comprises Clostridium baratii and LNnT.
In one example, the probiotic composition of the invention comprises Clostridium baratii and LNT.
In one example, the probiotic composition of the invention comprises Clostridium tedium and at least one prebiotic. For example, Clostridium baratii and LNnT, and/or LNT.
In one example, the probiotic composition of the invention comprises Clostridium tedium and 10 LNnT.
In one example, the probiotic composition of the invention comprises Clostridium tedium and LNT.
The probiotic composition may comprise a two or more Clostridium species selected from the group consisting of Clostridium perfringens, Clostridium baratii and Clostridium tedium. The probiotic composition may comprise two or more Clostridium species and at least one prebiotic.
For example, it may comprise Clostridium perfringens and Clostridium baratii and at least one prebiotic selected from the group consisting of: LNnT, 6SL, 2FL, DSLNT, LNFP-I and/or LNT. For example, it may comprise Clostridium perfringens and Clostridium baratii and at least one prebiotic selected from LNnT, and/or LNT.
For example, it may comprise Clostridium perfringens and Clostridium tedium and at least one prebiotic selected from the group consisting of: LNnT, 6SL, 2FL, DSLNT, LNFP-I and/or LNT. For example, it may comprise Clostridium perfringens and Clostridium tedium and at least one prebiotic selected from LNnT, and/or LNT.
For example, it may comprise Clostridium perfringens and Clostridium baratii and at least one prebiotic selected from the group consisting of: LNnT, 6SL, 2FL, DSLNT, LNFP-I and/or LNT. For example, it may comprise Clostridium perfringens and Clostridium baratii and at least one prebiotic selected from LNnT, and/or LNT.
For example, it may comprise Clostridium perfringens, Clostridium tedium and Clostridium boreal and at least one prebiotic selected from the group consisting of: LNnT, 6SL, 2FL, DSLNT, LNFP-I and/or LNT. For example, it may comprise Clostridium perfringens, Clostridium tertium and Clostridium baratii and at least one prebiotic selected from LNnT, and/or LNT.
In some examples, the probiotic composition may comprise additional bacteria, such as Bifidobacterium. Accordingly, the probiotic composition may comprise Bifidobacterium and one or more Clostridium species selected from the group consisting of Clostridium perfringens, Clostridium baratii and Clostridium tertium.
Prebiotic composition A prebiotic composition is also provided, which comprises at least one prebiotic and lacks the probiotic bacteria described herein (i.e. lacks Clostridium perfringens, Clostridium baratii and Clostridium tertium), wherein the prebiotic composition is for separate (simultaneous or consecutive) administration to the same subject as the probiotic composition of the invention.
Examples of prebiotics that may be included in the prebiotic composition are provided above and apply equally to this aspect.
The prebiotic composition comprises at least one prebiotic. In one example it comprises at least two, at least three, at least four, at least five, at least six, at least seven, at least eight, at least nine, at least ten, at least eleven, at least twelve, at least thirteen, at least fourteen etc prebiotics.
In one example, the prebiotic is a mammalian milk oligosaccharide (MMO), a precursor and/or a derivative thereof. In one example, the prebiotic is a human milk oligosaccharide (HMO), a precursor and/or a derivative thereof. These terms are defined elsewhere herein and apply equally to this aspect.
The HMO may be a fucosylated HMO, precursor, or derivative thereof. In another example, the HMO may be a sialylated HMO, precursor, or derivative thereof. In another example, the HMO may be a nonfucosylated neutral HMO, precursor, or derivative thereof. Non-limiting examples of these HMOs are provided elsewhere herein.
In one example, the HMO that is present in the prebiotic composition is selected from the group consisting of: lacto-N-tetraose (LNT), lacto-N-neotetraose (LNnT), disialyllacto-Ntetraose (DSLNT), lacto-N-fucopentaose I (LNFPI), 6'-sialyllactose (6SL) and 2-fucosyllactose (2FL); or a combination thereof.
In one example, the HMO that is present in the prebiotic composition is LNnT and/or 6SL.
In one example, a plurality of HMOs are present within the prebiotic composition of the invention. For example, the prebiotic composition may comprise at least two, at least three, at least four, at least five, at least six etc HMOs.
The HMOs present in the prebiotic composition may be a mixture of lacto-N-tetraose (LNT), 5 lacto-N-neotetraose (LNnT), disialyllacto-N-tetraose (DSLNT), lacto-N-fucopentaose I (LNFPI), 6'-sialyllactose (6SL), and/or 2-fucosyllactose (2FL), which are all naturally found in human milk.
In one example, the prebiotic composition comprises a milk selected from the group consisting of: human milk (HMO), bovine milk (BMO), ovine milk (OMO), equine milk (EMO) or caprine milk (CMO).
In one example, the prebiotic composition comprises human milk.
In a further example, the prebiotic composition comprises at least one HMO precursor. Non-limiting examples of HMO precursors are provided elsewhere herein.
Postbiotic composition The invention also provides a postbiotic composition for improving the gut health of a subject, the composition comprising: (a) at least one inanimate Clostridium species selected from the group consisting of: Clostridium perfringens, Clostridium baratii and Clostridium tedium; and/or (b) metabolites from at least one Clostridium species selected from the group consisting of: Clostridium perfringens, Clostridium baratii and Clostridium tedium.
In some examples, the postbiotic composition may further comprise: (a) at least one inanimate additional bacterial species, such as Bifidobacterium; and/or (b) metabolites of at least one additional bacterial species, such as Bifidobacterium. Accordingly, the postbiotic composition for improving the gut health of a subject may comprise: (a) at least one inanimate Bifidobacterium species and at least one inanimate Clostridium species selected from the group consisting of: Clostridium perfringens, Clostridium baratii and Clostridium tedium; and/or (b) metabolites from at least one Bifidobacterium species and metabolites from at least one Clostridium species selected from the group consisting of: Clostridium perfringens, Clostridium baratii and Clostridium tedium.
The term "postbiotic composition" as used herein is a general term to refer to a preparation of inanimate probiotic bacteria and/or their component parts (e.g., cellular debris) and/or their metabolites, wherein the composition is beneficial to the overall health of an individual (referred to as a subject herein) that consumes it. Postbiotic compositions are well known in the art. Postbiotic compositions that are described herein impart their beneficial effects in the gut of the subject, and therefore improve the gut microbiome of the subject. Postbiotic compositions may also be described as compositions that are utilised as a dietary supplement to alter the digestive tract microbiota of a subject in a beneficial manner without causing disease.
As would be clear to a person of skill in the art, a postbiotic composition comprises sufficient quantities of the inanimate probiotic bacteria (and/or their component parts (e.g., cellular debris) and/or their metabolites) to impart the required beneficial effect on the subject. Preparations of postbiotics typically contain the inanimate probiotic bacteria and can also retain microbe-produced substances, such as metabolites, proteins, or peptides, which may contribute to the overall health effect conferred by a postbiotic, but such microbe-produced substances are not essential to a postbiotic. The amount of inanimate probiotic bacteria (and/or their component parts (e.g., cellular debris) and/or their metabolites) in the postbiotic composition may be referred to as a therapeutically effective amount. Appropriate amounts can readily be identified by a person skilled in the art. Further details and examples are provided elsewhere herein.
The term "inanimate" as used herein refers to a bacterium that is no longer alive, or, in other words, dead or inactivated, and may comprise non-viable whole cells, cellular fragments, proteins, or peptides. Components derived from inanimate microorganisms may comprise byproducts such as metabolites. Appropriate metabolites are well known in the art and include SCFAs (such as butyrate, acetate, and/or propionate), secondary bile acid, and tryptophan metabolites (such as ILA).
Postbiotic compositions are provided herein which comprise at least one inanimate Clostridium species selected from the group consisting of: Clostridium perfringens, Clostridium baratii, and/or Clostridium tedium or at least one Clostridium perfringens metabolite, Clostridium baratii metabolite, and/or Clostridium tedium metabolite.
In one example, the postbiotic composition comprises at least one inanimate Clostridium species selected from the group consisting of: Clostridium perfringens, Clostridium baratii, and/or Clostridium tedium. The composition is beneficial to the overall health of an individual (referred to as a subject herein) that consumes it. The postbiotic composition comprising at least one inanimate Clostridium species selected from the group consisting of Clostridium perfringens, Clostridium baratii, and/or Clostridium tedium imparts its beneficial effects in the gut of the subject, and therefore improves the gut health of the subject.
The postbiotic composition may comprise at least two inanimate Clostridium species selected from the group consisting of: Clostridium perfringens, Clostridium baratii, and/or Clostridium tedium. For example, the postbiotic composition may comprise inanimate Clostridium perfringens and inanimate Clostridium baratii. In another example, the postbiotic composition may comprise inanimate Clostridium perfringens and inanimate Clostridium tedium. In another example, the postbiotic composition may comprise inanimate Clostridium baratii and inanimate Clostridium tedium. In another example, the postbiotic composition may comprise inanimate Clostridium perfringens, inanimate Clostridium baratii and inanimate Clostridium tedium.
The Clostridium can for example be made inanimate by killing at high temperature and pressure. It can for example also be removed by sterile filtration. Such methods are well known in the art.
In another example, the postbiotic composition comprises metabolites from at least one Clostridium species selected from the group consisting of: Clostridium perfringens, Clostridium baratii and Clostridium tedium. The composition is beneficial to the overall health of an individual (referred to as a subject herein) that consumes it. The postbiotic composition comprising metabolites from at least one Clostridium species selected from the group consisting of: Clostridium perfringens, Clostridium baratii and Clostridium tedium imparts its beneficial effects in the gut of the subject, and therefore improves the gut health of the subject.
The postbiotic composition may comprise metabolites from at least two Clostridium species selected from the group consisting of: Clostridium perfringens, Clostridium baratii, and/or Clostridium tedium. For example, the postbiotic composition may comprise metabolites from Clostridium perfringens and Clostridium baratii. In another example, the postbiotic composition may comprise metabolites from Clostridium perfringens and Clostridium tedium. In another example, the postbiotic composition may comprise metabolites from Clostridium baratii and Clostridium tedium. In another example, the postbiotic composition may comprise metabolites from Clostridium perfringens, Clostridium baratii and Clostridium tedium.
The terms "Clostridium perfringens metabolite", "Clostridium baratii metabolite", and "Clostridium tedium metabolite" as used herein refer to metabolites originating from the metabolic activity of these Clostridium species, respectively, in particular when grown on one or more HMOs as a carbon source.
A Clostridium perfringens metabolite, Clostridium baratii metabolite, and/or Clostridium tedium metabolite can for example be a short chain fatty acid (such as butyrate, acetate, and/or propionate), secondary bile acid, and/or tryptophan metabolites (such as ILA).
As would be clear to a person of skill in the art, a postbiotic composition may comprise metabolites from at least one Clostridium species selected from the group consisting of: Clostridium perfringens, Clostridium baratii, and/or Clostridium tedium due to the postbiotic composition being an in vitro cell culture product. In other words, the postbiotic composition may be generated by in vitro cell culture of the at least one Clostridium species selected from the group consisting of: Clostridium perfringens, Clostridium baratii, and/or Clostridium tedium. In vitro cell culture of the at least one Clostridium species selected from the group consisting of: Clostridium perfringens, Clostridium baratii, and/or Clostridium tedium may be for a sufficient time period (and under appropriate conditions) to generate metabolites from the one or more Clostridium species. Typically, the in vitro cell culture of the at least one Clostridium species selected from the group consisting of: Clostridium perfringens, Clostridium baratii, and/or Clostridium tedium may be performed by culturing the at least one Clostridium species with a prebiotic for a sufficient time period (and under appropriate conditions) to generate the metabolites. For example, the in vitro cell culture of the at least one Clostridium species selected from the group consisting of: Clostridium perfringens, Clostridium baratii, and/or Clostridium tedium may be performed by culturing the at least one Clostridium species with a MMO for a sufficient time period (and under appropriate conditions) to generate the metabolites.
In one example, the in vitro cell culture of the at least one Clostridium species selected from the group consisting of: Clostridium perfringens, Clostridium baratii, and/or Clostridium tedium may be performed by culturing the at least one Clostridium species with a HMO for a sufficient time period (and under appropriate conditions) to generate the metabolites.
The postbiotic composition may then be generated by removing the Clostridium species from the in vitro cell culture after the metabolites have been generated. Any appropriate method may be used to remove the Clostridium species, for example filtration, centrifugation etc. A non-limiting example of how to generate an appropriate postbiotic is provided in the examples section below.
A postbiotic composition may therefore be generated by culturing the appropriate Clostridium species selected from the group consisting of: Clostridium perfringens, Clostridium baratii, and/or Clostridium tedium anaerobically in the presence of a suitable MMO under appropriate culture conditions.
For example, Clostridium perfringens may be cultured in the presence of a HMO selected from: LNnT, 6SL, 2FL, DSLNT, LNFP-I and/or LNT under appropriate culture conditions. For example, Clostridium perfringens may be cultured in the presence of a HMO selected from: LNnT, 6SL, 2FL, DSLNT, and/or LNT under appropriate culture conditions. For example, Clostridium perfringens may be cultured in the presence of a HMO selected from: LNnT, 6SL, 2FL, and/or DSLNT under appropriate culture conditions. For example, Clostridium perfringens may be cultured in the presence of a HMO selected from: LNnT, 6SL and/or DSLNT under appropriate culture conditions. For example, Clostridium perfringens may be cultured in the presence of a HMO selected from: LNnT and/or 6SL under appropriate culture conditions. For example, Clostridium perfringens may be cultured in the presence of LNnT under appropriate culture conditions. For example, Clostridium perfringens may be cultured in the presence of 6SL under appropriate culture conditions.
For example, Clostridium baratii may be cultured in the presence of a HMO selected from: LNnT, and/or LNT under appropriate culture conditions. For example, Clostridium baratii may be cultured in the presence of LNnT. For example, Clostridium baratii may be cultured in the presence of LNT under appropriate culture conditions.
For example, Clostridium tedium may be cultured in the presence of a HMO selected from: LNnT, and/or LNT under appropriate culture conditions. For example, Clostridium tedium may be cultured in the presence of LNnT. For example, Clostridium tedium may be cultured in the presence of LNT under appropriate culture conditions.
For example, Clostridium perfringens and Clostridium baratii may be cultured in the presence of a HMO selected from: LNnT, 6SL, 2FL, DSLNT, LNFP-I and/or LNT under appropriate culture conditions. For example, Clostridium perfringens and Clostridium baratii may be cultured in the presence of a HMO selected from: LNnT, and/or LNT under appropriate culture conditions.
For example, Clostridium perfringens and Clostridium tedium may be cultured in the presence of a HMO selected from: LNnT, 6SL, 2FL, DSLNT, LNFP-I and/or LNT under appropriate culture conditions. For example, Clostridium perfringens and Clostridium tedium may be cultured in the presence of a HMO selected from: LNnT, and/or LNT under appropriate culture conditions.
For example, Clostridium perfringens, Clostridium tedium and Clostridium baratii may be cultured in the presence of a HMO selected from: LNnT, 6SL, 2FL, DSLNT, LNFP-I and/or LNT under appropriate culture conditions. For example, Clostridium perfringens, Clostridium tedium and Clostridium baratii may be cultured in the presence of a HMO selected from: LNnT, and/or LNT under appropriate culture conditions.
Appropriate culture conditions will be readily identifiable to a person of skill in the art (e.g. anaerobically at between 35 -39°C, for a period of at least 2 days, typically at least 5 or 6 days), optionally whilst shaking (e.g. at 130 rpm).
Appropriate culture conditions include anaerobic culture at around 37°C, for a period of at least 2 days, typically at least 5 or 6 days), optionally whilst shaking (e.g. at 130 rpm).
The postbiotic composition may then be generated by removing the Clostridium species from the cell culture. Any appropriate method may be used to remove the Clostridium species, for example filtration, centrifugation etc. Centrifugation may be for at least 5000g for at least 5 minutes at about 4°C. Fitration may be using a 0.22 pm filter. Centrifugation for at least 5000g for at least 5 minutes at about 4°C may be combined with fitration using a 0.22 pm filter.
The postbiotic composition may alternatively be generated by heat treating the Clostridium species in the cell culture to ensure that they are inanimate.
The postbiotic composition may include the entire cell culture, with the exception of live Clostridium species.
Formulation of the composition The compositions provided herein may be administered to the subject in any suitable format and by any appropriate means. Typically, the compositions described herein are for oral use. Accordingly, the compositions described herein are typically comestible (also referred to as edible herein). Suitably, the composition may be in the form of a liquid medium, gel medium, powder, dissolvable medium, chewable medium, or any other ingestible medium. This includes, without limitation, tablets, capsules (including a gelatin capsules), pills, caplets, yogurts, freeze dried products, granules, syrups, drinks, food and the like. Suitable ways of preparing such compositions are well known in the art.
In one example the composition is in a powder, liquid or solid form.
Compositions described herein may also contain conventional food supplement fillers and extenders such as, for example, a flour, a binder, a neutraceutical compound or formulation, an amino acid, a vitamin, or a mineral.
The bacteria described herein may be dried with a food product as described in W098/10666, which is incorporated herein by reference in its entirety. Accordingly, the Clostridium species may be dried at the same time with juices, milk-based products or vegetable milks, for example, yielding a dried product already comprising the Clostridium species. This product may later be reconstituted with an aqueous liquid such as milk or water.
The composition may be a milk product. A milk product is any comestible composition that comprises animal milk (obtained from a mammal) or a milk substitute (such as plant-based milk products). The milk product may be in liquid, solid or powder form.
A milk product may comprise animal milk or a milk substitute. Examples of animal milk include, but are not limited to, bovine, goat, sheep, camel, donkey, and reindeer milk. Human milk is also encompassed by this term. A non-human or non-animal milk product may also be referred to as a "milk substitute" herein. Milk substitutes therefore include plant milk, such as almond, soy, oat, coconut, pea, hemp, spelt, rice, pistachio, walnut, macadamia, flax, banana, cashew, hazelnut, quinoa, and sesame milk. Milk substitutes also include modified milk, such as infant formula.
A milk product includes, but is not limited to, cheese, butter, cream, yoghurt, ghee, condensed milk, ice cream, buttermilk, milkshake, and whey etc. A milk product also includes milk-based drinks or powders; and drinks or powders based on milk substitutes.
The milk product may comprise human donor breast milk.
The milk product may also be a milk product that has been generated in vitro. For example, it may be milk that has been produced by mammary organoids.
The milk product may be a modified milk product. In other words, instead of comprising naturally occurring milk, it may comprise modified milk that has been adapted for the subject of interest. An example of a modified milk product is infant formula, which comprises cow's milk that has been treated to make it more suitable for the subject of interest (e.g. human infants).
In one example, the composition is an infant milk product that is selected from the group consisting of: infant formula, a starter infant formula, a follow-on or follow-up infant formula, a baby food, or an infant cereal composition.
As used herein, the terms "formula milk", "formula", "baby formula", "growing-up milk", or "infant formula" are interchangeable and are intended to represent the substitute for human or animal breast milk which is given to infant humans or animals (0-36 months of age). The infant formula may be powdered, concentrated liquid formula, or ready-to-use formula.
A milk product is therefore provided herein that comprises a probiotic composition for improving the gut microbiome and/or gut health of a subject, the composition comprising at least one Clostridium species selected from the group consisting of: Clostridium perfringens, Clostridium baratii and Clostridium tedium.
A milk product is also provided herein that comprises a postbiotic composition for improving the gut microbiome and/or gut health of a subject, the composition comprising at least one inanimate Clostridium species selected from the group consisting of: Clostridium perfringens, Clostridium baratii and Clostridium tedium.
A milk product is also provided herein that comprises a postbiotic composition for improving the gut microbiome and/or gut health of a subject, the composition comprising metabolites from at least one Clostridium species selected from the group consisting of: a Clostridium 10 perfringens, Clostridium bare& and Clostridium tedium.
A milk product may also be a prebiotic composition as described herein.
In one example the probiotic and/or postbiotic compositions provided herein reduce pathogen overgrowth in the gut, promote gut development, promote gut maturation, promote immunity, decrease gut inflammation, and/or promote enteral feeding tolerance in the subject.
Improving the out microbiome and/or gut health of a subject The compositions provided herein are for improving the gut microbiome and/or gut health of a subject. In this context, the terms "improving" or "improve" refer to altering the bacterial composition of the gut microbiome in a beneficial way or altering the gut health of the subject in a beneficial way. In the context of improving the gut microbiome, it refers to altering the relative abundance of a variety of bacterial species in the gut microbiome of the subject in a beneficial manner (e.g. to move from a state of dysbiosis of the gut microbiome towards a state of eubiosis). Eubiosis of the gut microbiome refers to the healthy and balanced state of bacterial populations within the gut. Dysbiosis of the gut microbiome is a negative alteration in the relative abundance of bacterial species in the gut and is associated with disease.
Dysbiosis may also be referred to as a disturbance of eubiosis. Dysbiosis of the gut microbiome can be caused by infections, inflammation, diet, xenobiotics (including antibiotics, drugs, food additives), and birth route. Methods of determining whether the gut microbiome has been improved, and identifying eubiosis and dysbiosis of the gut microbiome are well known. For example, see US2022/0313759, which provides several assays for determining an improvement in the gut microbiome, which are incorporated herein in their entirety.
Methods for improving the gut microbiome and/or gut health of a subject are also well known and include consumption of exogeneous prebiotics, probiotics and/or postbiotics.
Improvement of the gut microbiome and/or improving gut health can provide one or more beneficial effects. For example, it may reduce pathogen overgrowth in the gut, promote gut development, promote gut maturation, promote immunity, decrease gut inflammation, and/or the promote enteral feeding tolerance in the subject. Each of these beneficial effects can readily be identified by a person of skill in the art, for example by comparing one or more of pathogen overgrowth, gut development, gut maturation, gut inflammation, enteral feeding tolerance etc in the subject before and after compositions of the invention have been consumed by the subject for an appropriate length of time. Appropriate consumption regimens for probiotics to have a beneficial effect are known in the art.
Improvement of the gut microbiome (as well as the associated health benefits described above) can in turn lead to subsequent improvements for the subject such as an improved eye sight (e.g., an improvement in retinopathy of prematurity (ROP)) or improved respiratory tract function (e.g. improved respiratory tract function in subjects with Bronchopulmonary dysplasia (BPD)). Enrichment of Enterobacteriaceae in the gut microbiome of preterm infants has been associated with ROP (Skondra et al., J AAPOS. 2020 Aug; 24(4): 236-238) and different probiotics have been shown to reduce the relative abundance of species from within this family (e.g., Klebsiella) (Beck at al., Nature Microbiologyvolume 7, pages 1525.-1535 (2022)).
Enrichment of Enterobacteriaceae and reduced diversity in the gut microbiome of preterm infants has also been associated with BPD (Chen et al., m J Perinatol 2021; 38(11): 11421149).
Accordingly, the compositions provided herein may be of benefit to subjects with retinopathy of prematurity (ROP) and Bronchopulmonary dysplasia (BPD)). In such cases, the composition may be used as a supplement by these subjects, to improve the symptoms and signs or disease.
The compositions described herein may also be used to reduce the relative abundance of one or more pathobionts in the gut of a subject. For example, the compositions may be used to inhibit the growth of one or more pathobionts in the gut of a subject. The compositions may be used to inhibit the growth of e.g. one or more of E. colt, E. faecalis, K. oxytoca and/or K. pneumoniae. This is particularly applicable to the postbiotic compositions of the invention, as shown in the examples section below.
The compositions described herein may also be used to reduce Enterobacteriaceae relative 30 abundance.
The compositions described herein may also be used in the treatment or prevention of a gastrointestinal disorder, allergy, nappy rash, colic, or a symptom thereof in a subject.
The term "gastrointestinal disorder" as used herein refers to disorders and diseases of the digestive system (namely the oesophagus, stomach, small intestine, large intestine, and/or rectum) or accessory organs (such as the liver, pancreas, and gallbladder). It also refers to disorders that originate from problems with the digestive system. A subject with a gastrointestinal disorder will commonly experience symptoms including, but not limited to, diarrhoea, abdominal pain, gastrointestinal bleeding, and/or intestinal obstruction. A gastrointestinal disorder can be either a functional gastrointestinal disorder or an organic gastrointestinal disorder. A functional gastrointestinal disorder is not associated with any physiological changes. Non-limiting examples of functional gastrointestinal disorders include irritable bowel syndrome (IBS), gastroesophageal reflux disease, functional dyspepsia, functional nausea and vomiting, functional abdominal pain, functional constipation, and faecal incontinence.
An organic gastrointestinal disorder has measurable physiological changes and often results from intestinal inflammation. Non-limiting examples of organic gastrointestinal disorders include Crohn's disease, ulcerative colitis, coeliac disease, inflammatory bowel disease (IBD), chronic gastrointestinal infections, microscopic colitis, intestinal inflammation, colitis, necrotising enterocolitis (NEC), late-onset sepsis, and focal intestinal perforation.
The compositions described herein may also be used in the treatment or prevention of a gastrointestinal disorder, selected from the group consisting of: intestinal inflammation, irritable bowel syndrome, colitis, necrotising enterocolitis, focal intestinal perforation, late-onset sepsis, and inflammatory bowel disease.
The term "intestinal inflammation" as used herein covers both acute and chronic inflammation of the tissues in a subject's digestive tract, and is classified by redness (rubor), swelling (tumour), pain (dolor), and/or loss of function (functio laesa) as a result of the subject's body's reaction against injury and infection orchestrated by the immune system.
The term "irritable bowel syndrome" as used herein refers to a group of symptoms that occur together, including, but not limited to, recurrent stomach cramps, bloating, distension, diarrhoea, and/or constipation.
The term "colitis" as used herein refers to inflammation of the lining of the large intestine (colon). Any disease or disorder which results in acute or chronic inflammation of the large intestine falls under this term. Non-limiting causes of colitis include infections caused by viruses, parasites or bacteria, Crohn's disease, ulcerative colitis, necrotising enterocolitis. Colitis commonly manifests as abdominal pain and bloating, bloody stools, tenesmus, dehydration, diarrhoea, and/or fever.
The term "necrotising enterocolitis (NEC)" as used herein refers to the acute inflammatory disorder responsible for necrosis of the intestinal tissue. NEC predominantly develops in preterm infants and rarely affects infants born at term or adults. Infants usually develop it within two to six weeks after birth. NEC can be mild or severe, with the latter experiencing life-threatening symptoms.
The term "intestinal perforation" as used herein refers to the loss of continuity of the gastrointestinal or bowel wall and is a common complication of a number of diseases or disorders. Non-limiting examples resulting in intestinal perforation comprise appendicitis, cancers affecting the digesting system, cancers of the digestive system, diverticulitis, hernias, bowel obstruction, gall stones, colitis, trauma, ischaemia, inflammation, and necrotising enterocolitis. In the context of newborns and infants, a specific example is focal intestinal perforation (FIP), which is a single intestinal perforation that is typically found at the terminal ileum.
The term "sepsis" is defined as the dysregulated host response to infection encompassing a spectrum of disease/disorder, ranging from minor signs and symptoms to life-threatening organ dysfunction and death. Infections that lead to sepsis most often start in the lung, urinary tract, skin, or gastrointestinal tract. Sepsis may progress to septic shock, which comprises a dramatic drop in blood pressure which can damage vital organs and can be fatal. The term "early-onset sepsis" as used herein refers to sepsis onset most often appearing within 24 to 48 hours of birth, and has commonly contracted the infection from the mother before or during delivery. The term "late-onset sepsis" as used herein refers to sepsis onset after 72 hours and up to 90 days of life and is the pathogens responsible are usually acquired from the environment during or after delivery. It is more common in preterm infants.
The term "inflammatory bowel disease" (IBD) is a term used to describe two conditions: ulcerative colitis and Crohn's disease. Ulcerative colitis and Crohn's disease are long-term conditions that involve inflammation of the gut. Ulcerative colitis only affects the colon (large intestine). Crohn's disease can affect any part of the digestive system, from the mouth to the anus. Symptoms of IBD include pain, cramps or swelling in the tummy; recurring or bloody diarrhoea; weight loss and extreme tiredness.
The compositions provided herein result in an improvement of the gut microbiome and/or gut health of a subject, which can provide one or more beneficial effects. For example, it may reduce pathogen overgrowth in the gut, promote gut development, promote gut maturation, promote immunity, decrease gut inflammation, and/or the promote enteral feeding tolerance in the subject.
The terms "small intestinal bacterial overgrowth", "SIBO", and "pathogen overgrowth" as used herein are interchangeable and refer to the presence of excess colonic bacteria in the small intestine resulting in an unbalanced microbiota. Commonly present in SIBO are Escherichia coli, Aeromonas species, and Klebsiella species, but Bacteroides, Clostridium, Peptostreptococcus, and Micrococcus species are also responsible.
The term "gut development' as used herein refers to the process of establishing the microbiota from birth. A similar term "gut maturation" as used herein is intended to refer to the process of the maturing of the microbiota composition to an adult-like composition and function between three and five years of age.
The term "immunity" as used herein broadly refers to the subject's state of being insusceptible or resistant to a pathogen or infectious disease. Immunity is governed by the immune system, a system of defence comprising a complex network of organs, cells, tissues, and other components, such as proteins, which act together to help protect the subject. The three types of immunity are innate, adaptive, and passive. Innate immunity is the system with which you were born with and is characterised by being non-specific. Non-limiting examples of innate immunity comprise stomach acid, the skin barrier, mucus, and enzymes in saliva and skin oils. Adaptive immunity is the system conferring acquired immune responses or active' immunity, resulting in immunological memory after an initial response to, usually, a pathogen. Non-limiting examples of adaptive immunity comprise an immature B cell becoming a plasma cell and producing specific antibodies to an epitope on an antigen. Passive immunity is immunity provided by the administering of exogenous immune components (either naturally or artificially) which provide short-term protection to the subject. Non-limiting examples of passive immunity comprise a baby receiving its mother's antibodies through the placenta or breast milk (natural passive immunity) or the administering of antibodies to neutralise a toxin (artificial passive immunity).
The term "enteral feeding" as used herein refers to nutrition taken through the mouth or through a tube that goes directly to the stomach or small intestine. The latter meaning is often used in a medical setting. In turn, the term "enteral feeding tolerance" as used herein is intended to refer to the absence of an adverse reaction to the delivery of nutrition or food taken through the mouth or through a tube that goes directly to the stomach or small intestine.
The compositions provided herein may also be used to treat or prevent an allergy in a subject. The term "allergy" as used herein refers to a subject's immune system's hypersensitive response to a foreign, but inert, substance (i.e., an allergen) in the environment. Allergies can occur on a range of severity, from a blocked nose to anaphylaxis. Allergic reactions can be mediated by IgE or be non-IgE in nature. Non-limiting examples of allergens can include food, insect stings, latex, and drugs. Non-limiting examples of food allergens in allergenic food include celery, gluten containing cereal (such as barley, wheat, and oats), crustaceans (such as crabs, prawns, and lobsters), fish, milk, molluscs (such as mussels and oysters), eggs, mustard, peanuts, sesame, soybeans, sulphur dioxide and sulphites and tree nuts (such as hazelnuts, walnuts, almonds, cashews, pecans, macadamia nuts, pistachio, and brazil nuts).
A further example of an allergy is cows' milk allergy (CMA), also called cows' milk protein allergy, is one of the most common childhood food allergies. CMA typically develops when cows' milk is first introduced into the baby's diet either in formula or when your baby starts eating solids. More rarely, it can affect babies who are exclusively breastfed because of cows' milk from the mother's diet passing to the baby through breast milk. There are 2 main types of CMA: immediate CMA -where symptoms typically begin within minutes of having cows' milk - delayed CMA -where symptoms typically begin several hours, or even days, after having cows' milk.
Cows' milk allergy can cause a wide range of symptoms, including: - skin reactions -such as an itchy rash or swelling of the lips, face and around the eyes digestive problems -such as stomach ache, vomiting, colic, diarrhoea or constipation hay fever-like symptoms -such as a runny or blocked nose eczema that does not improve with treatment Occasionally CMA can cause severe allergic symptoms that come on suddenly, such as swelling in the mouth or throat, wheezing, cough, shortness of breath, and difficult, noisy breathing.
The compositions provided herein may also be used to treat or prevent nappy rash in a subject. The term "nappy rash" as used herein refers to the acute inflammatory reaction of the skin in contact with a nappy. It can be characterised by inflamed, irritated, red, and/or dry skin in contact with the nappy and is primarily characterised as an irritant contact dermatitis. It is commonly caused by the prolonged skin contact with the subject's urine or faeces, skincare preparations, or detergents and fabric softeners.
The compositions provided herein may also be used to treat or prevent an colic in a subject.
The terms "colic" and "infantile colic" as used herein are interchangeable and refer to the paroxysms of crying of a healthy infant when there is no obvious cause for the infant to be crying. Colic is a pattern of crying, which occurs daily and often at the same time of day which occurs in up to a quarter of newborn babies. It is most common during the first six weeks of life and will usually resolve by four to six months independent of therapeutic intervention.
The compositions provided herein may reduce the proportion of pathogenic bacteria in the microbiome of the subejct. In some embodiments, the pathogenic bacteria are Enterobacteriaceae (e.g., one or more of Salmonella, E. coli, or Klebsiella). In some examples, the pathogenic bacteria are reduced by greater than 10%, 15%, 25%, 50%, 75%, 80%, or 85% by the treatment.
The compositions provided herein may reduce the antibiotic resistance gene load in the subject. One or more genes of the antibiotic resistance gene load may be reduced by greater than 10%, 15%, 25%, 30%, 45%, 50%, 75% or 85%. In some examples, the compositions provided herein may reduce the levels of lipopolysaccharide (LPS) and/or pathogenic bacteria in the gut of the subject.
The compositions provided herein may reduce the frequency of bowel movements in the subject as compared to a dysbiotic subject. In some examples, the stool composition of an subject be altered as compared to a dysbiotic subject. The firmness/consistency of the stool composition of the subject can be increased as compared to a dysbiotic subject. In some examples, the stool can be less watery.
Subjects The compositions described herein may be of benefit to any appropriate subject. The term "subject' as used herein is intended to include humans and animals. The terms "subject", "individual", and "patient" are used herein interchangeably. Examples of subjects include mammals, e.g., humans, dogs, cows, horses, pigs, sheep, goats, cats, mice, rabbits, rats, and transgenic non-human animals. In some examples, subjects include companion animals, e.g. dogs, cats, rabbits, and rats. In some examples, subjects include livestock, e.g., cows, pigs, sheep, goats, and rabbits. In some examples, subjects include thoroughbred or show animals, e.g. horses, pigs, cows, and rabbits. The subject may be an infant.
In some examples, the subject is a human. The subject may be a human infant. The term "human infant" as used herein is intended to refer to a child in the first year of life.
The subject may be less than four weeks old, which may be referred to herein as "neonate".
The subject may be born at term or preterm. The subject may be born alive before 37 weeks of pregnancy, which may be referred to herein as a "preterm infant".
The subject may be an infant who is small for their gestational age, which comprises an infant born with a birth weight less than the 10th centile (based on age).
The subject may be an infant born by Caesarean-section (C-section).
In some examples, the subject may be an infant prior to the NEC risk period. In some examples, the subject may be a newborn during the NEC risk period. In some examples, a subject may be a newborn post-NEC risk period. In this context, the NEC risk period may be defined as the first 10 to 40 days of life.
In some examples, the subject may be an infant that is in (staying in) an NICU (neonatal intensive care unit). In some examples, the subject may be an infant that has been discharged from NICU within the last month.
In some examples, the subject may be 0-3 months old.
In some examples, a subject may be 3-12 months old.
In some examples, a subject may be from 1-5 years old. In some examples, the subject may be 5-13 years old. In some examples, the subject may be 13+ years old. In some examples the subject may be an adult (18+).
In some examples the subject may be elderly (65+).
The subject may be an infant that has been hospitalised and/or is undergoing antibiotic treatment. Infants, including preterm infants on NICU, spending prolonged time in hospital will get colonised by higher levels of hospital acquired pathobiont bacteria and therefore in particular require a stabilization and/or improvement of the gut microbiome.
In any examples described herein, the subject can be dysbiotic. "Dysbiotic" refers to the condition of dysbiosis (also called dysbacteriosis) and is characterized by a disruption to the microbiome resulting in an imbalance in the microbiota, changes in their functional composition and metabolic activities, or a shift in their local distribution. Dysbiosis can refer to any flora such as the skin flora, gut flora, or vaginal flora.
In particular, the subject can be dysbiotic with regard to their gut microbiome. Examples of such subjects may be those that are undergoing antibiotic treatment.
Dysbiosis in a subject, especially an infant, can be observed by the physical symptoms of the subject (e.g., diarrhea, digestive discomfort, inflammation, etc.) and/or by observation of the presence of free sugar monomers in the feces of the subject, an absence or reduction in specific Bifidobacteria populations, and/or the overall reduction in measured SCFA; more specifically, acetate and lactate. Additionally, the infant may have an increased likelihood of becoming dysbiotic based on the circumstances in the environment surrounding them (e.g., an outbreak of disease in the surroundings of the subject, formula feeding, cesarean birth, etc.). Dysbiosis in an infant can further be revealed by a low level of SCFA in the feces of said infant.
As an example, a dysbiotic infant may have (a) a watery stool, (b) Clostridium difficile levels of greater than 106 cfu/g feces, greater than 107 cfu/g feces, or greater than 108 cfu/g feces, (c) Enterobacteriaceae at levels of greater than greater than 106, greater than 107, or greater than 108 cfu/g feces, and/or (d) a stool pH of 5.5 or above, 6.0 or above, or 6.5 or above.
As used herein, the terms "gut microbiome", "gut flora", "gastrointestinal flora", "intestinal flora", "intestinal microbiome", "microbiota", and "microbiome" are interchangeable and are intended to represent the bacterial community present in the digestive tract of the subject. It is meant to reflect the variety of commensal and mutualistic species, and relative abundance of said bacterial species found in a healthy subject. It is the gut microbiota which functions to ferment non-digestible substrates like dietary fibres and endogenous intestinal mucus (prebiotics), metabolise nutrients, xenobiotic and drugs, maintain structural integrity of the mucosal barrier, immunomodulation, and confer pathogenic resistance.
Methods of administration Typically, the compositions provided herein are for oral administration. However, other suitable routes of administration of the composition described herein may be via suppositories or feeding tubes directly into the digestive tract, including but not limited to, a jejunostomy tube, gastrostomy tube, or a nasojejunal tube.
The compositions provided herein are intended as a supplement and may be administered regularly for effects to be maintained or persist. After administration, for example by ingestion, probiotics typically adhere to a tissue of the host, such as the digestive tract. Once attached, the desirable bacteria are capable of multiplying and colonising, thereby altering the microbiota of a subject.
Probiotics can be mixed with various ingredients to create a product for consumption.
The compositions provided herein are for administration to the subject at an appropriate (e.g. therapeutically effective) dose. Appropriate doses are readily identifiable by a person of skill in the art.
In one example the probiotic composition provided herein comprises at least 0.5 x 106 CFU of the Clostridium species per dose. As would be clear, for compositions that comprise more than one Clostridium species, the probiotic composition provided herein may comprise at least 0.5 x 109 CFU of each Clostridium species per dose.
The term "dose" as used herein refers to the quantity of the Clostridium species administered or taken with one total serving, i.e. it resembles the total intake independent of the volume administered or taken, with one portion. Thereby "one portion" as used herein refers to the recommended amount of the composition independent of the form. It may be a certain amount of tablets, pills, capsules or a certain amount of a (pre-packed) syrup, (pre-packed) powder, (pre-packed) solid beverage, (pre-packed) soup, or (pre-packed) liquid suspension.
The term CFU (colony forming unit) as used herein refers to a single, viable propagule that produces a single colony (a population of the cells visible to the naked eye) on an appropriate semisolid growth medium. Methods for determining the number of CFUs are well known in the art.
In one example the composition comprises at least 0.6 x 109 CFU per dose per strain, at least 0.6 x 109 CFU per dose per strain, at least 1 x 109 CFU per dose per strain, at least 2 x 109 CFU per dose per strain, at least 3 x 109 CFU per dose per strain, at least 4 x 109 CFU per dose per strain, at least 5 x 109 CFU per dose per strain, at least 6 x 109 CFU per dose per strain, at least 7 x 109 CFU per dose per strain, or at least 8 x 109 CFU per dose per strain.
The CFU per dose can vary between the two or three species or can be the same.
In some examples, the composition is provided to the subject on a daily basis comprising from 0.1 billion to 500 billion cfu of bacteria/day. For example, the composition that is provided on a daily basis can include from 1 billion to 100 billion cfu/day or from 5 billion to 20 billion cfu/day. The composition may be provided on a daily basis for at least 2, at least 5, at least 10, at least 20, or at least 30 days. The recipient of the treatment can be a human infant.
Based on the subject's condition the skilled person would be able to choose the right dosage for each strain.
The composition can be administrated orally or by other suitable routes. When administered orally the composition may be in the form of capsules, tablets, powders, granules, solutions, or suspensions etc. The Clostridium species can be mixed with conventional excipients, such as gelatin, starch, lactose, magnesium stearate, talc, gum arabic and the like. It may also be advantageous to use less conventional excipients that, for example, make it possible to increase the ability of Clostridium species to be active in the gut. For example, cellobiose, maltose, mannose, salicine, trehalose, amygdalin, arabinose, melobiose, rhamnose and/or xylose may be added. This list is not exhaustive and the substrates may be chosen or changed depending on circumstances. Additionally, a capsule, pill, tablet, or syrup for oral administration should be stored in a manner so as to preserve its efficacy. Methods of storage for probiotic compositions include but are not limited to refrigeration, freezing, and storing at room temperature. If stored at room temperature, the Clostridium species or compositions thereof should be stored in an air tight container.
The probiotic Clostridium species should arrive intactly in the small and large intestine the latter of which may be colonized. The Clostridium species may be microencapsulated or enterically coated to provide a release profile that targets replacement or alteration of live Clostridium species at a pre-determined location within the gastrointestinal tract of a subject. Such microencapsulation formulations and techniques may protect the live Clostridium species from the digestive actions of the stomach, duodenum, and jejunum of the intestine and allow administration, delivery or release to the gut or ileum of a subject. Microencapsulated live Clostridium species and compositions thereof may be in various dosage forms, and they can be co-administered with drugs, foods, nutrients, vitamins, other beneficial substances, prebiotics, and other therapeutic agents such as pH encapsulated glucose, lipids or proteins that release in the distal small intestine at pH values between 7.0 and 8.0 in an amount sufficient to achieve the desired effect in a subject. For example, at least two coatings may be used to cover a tablet or capsule like form comprising the Clostridium species, wherein the outside coating is degraded in a pH environment of 5 to 6 and the inside coating is degraded in a pH environment of about 7 thereby releasing the Clostridium species in the ileum area. An exemplary coating may include one or more of poly(dl-lactide-co-glycolide) chitosan, casein, chitosan (Chi) stabilized with PVA (poly-vinylic alcohol), a lipid, an alginate, carboxymethylethylcellulose (CMEC), cellulose acetate trimellitiate (CAT), hydroxypropylmethyl cellulose phthalate (HPMCP), hydroxypropylmethyl cellulose, ethyl cellulose, color con, food glaze and mixtures of hydroxypropylmethyl cellulose and ethyl cellulose, polyvinyl acetate phthalate (PVAP), cellulose acetate phthalate (CAP), shellac, copolymers of methacrylic acid and ethyl acrylate, and copolymers of methacrylic acid and ethyl acrylate to which a monomer of methylacrylate has been added during polymerization, and other methacrylic resins that are commercially available under the tradename Eudragit® (Rohm Pharma; Westerstadt, Germany), including Eudragit® L30D-55 and L100-55 (soluble at pH 5.5 and above), Eudragit® L-100 (soluble at pH 6.0 and above), Eudragit® S (soluble at pH 7.0 and above, as a result of a higher degree of esterification), and Eudragits® NE, RL and RS (water-insoluble polymers having different degrees of permeability and expandability); vinyl polymers and copolymers such as polyvinyl pyrrolidone, vinyl acetate, vinylacetate phthalate, vinylacetate crotonic acid copolymer, and ethylene-vinyl acetate copolymer; enzymatically degradable polymers such as azo polymers, pectin, amylose and guar gum; and zein. Combinations of different coating materials may also be used. Multi-layer coatings using different polymers may also be applied. The preferred coating weights for particular coating materials may be readily determined by those skilled in the art by evaluating individual release profiles for tablets, beads and granules prepared with different quantities of various coating materials. It is the combination of materials, method and form of application that produce the desired release.
The compositions described herein may be administered once or more per day to a subject. The administration amount can vary depending on the body weight of the subject, the severity of a subject's condition, the subject's desired outcomes, supplemental active ingredients included and microorganisms used therein. In addition, it is possible to divide up a daily administration amount and to administer continuously, if needed. Suitable dosages of Clostridium species or compositions thereof may be provided orally at a dosage rate of at least 10 milligrams to at least 1000 milligrams per day. The dosage rate, effective as a food supplement and for establishing or increasing Clostridium species in the intestinal tract of a subject may be determined by known methods in the art.
In some examples, Clostridium species or compositions thereof may be administered 1, 2 3, 4, 5 or more times per week for a specified time period. For example, the specified time period may be at least one week. In some examples, the specified time period may be at least 1, at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8 weeks or more. In some examples, administration may be continuous, for example a subject may continually take doses of Clostridium species or compositions thereof even after effects the beneficial effects have been achieved. In the context of medical conditions and diseases that may reduce muscle mass and/or strength, continuous administration may help prevent or reduce the muscle wasting effects of the condition or disease.
An "effective amount" refers to an amount of a Clostridium species sufficient to achieve the desired function or activity in the subject and is acceptable to the subject (i.e. does not cause adverse effects in the subject). For example, an effective amount may be an amount of Clostridium species that provides some improvement or benefit to a subject having a disease, condition, or disorder in which a loss of muscle mass and/or muscle strength occurs. Thus, an "effective amount" or "therapeutically effective amount" with reference to therapeutic uses and methods is an amount that provides some alleviation, mitigation, and/or decrease in at least one clinical symptom of the disease, condition, or disorder in which a loss of muscle mass and/or muscle strength occurs. Those skilled in the art will appreciate that therapeutic effects need not be complete or curative, as long as some benefit is provided to the subject.
The effective amount of Clostridium species and/or the metabolite thereof in the preparation of the compositions described herein can vary depending on the mode of administration and the requirements of the subject. For example, the severity of a subject's condition or the subject's desired result.
Throughout the description and claims of this specification, the words "comprise" and "contain" and variations of them mean "including but not limited to", and they are not intended to (and do not) exclude other moieties, additives, components, integers or steps.
Throughout the description and claims of this specification, the singular encompasses the plural unless the context otherwise requires. In particular, where the indefinite article is used, the specification is to be understood as contemplating plurality as well as singularity, unless the context requires otherwise.
Features, integers, characteristics, compounds, chemical moieties or groups described in conjunction with a particular aspect, embodiment or example of the invention are to be understood to be applicable to any other aspect, embodiment or example described herein unless incompatible therewith.
Unless defined otherwise herein, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. For example, Singleton and Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d Ed., John Wiley and Sons, NY (1994); and Hale and Marham, The Harper Collins Dictionary of Biology, Harper Perennial, NY (1991) provide those of skill in the art with a general dictionary of many of the terms used in the invention. Although any methods and materials similar or equivalent to those described herein find use in the practice of the present invention, the preferred methods and materials are described herein. Accordingly, the terms defined immediately below are more fully described by reference to the Specification as a whole.
Aspects of the invention are demonstrated by the following non-limiting examples.
Examples Example I
Experimental Design To identify novel bacteria that can use HMOs as growth substrates, a feature widely reported to be associated with potentially beneficial bacteria (referred to as probiotic), stool samples were collected from preterm infants in the neonatal intensive care unit at Newcastle, UK. Preterm infant stool samples underwent microbiological culture to isolate a range of novel bacterial isolates. Growth curves were then performed to determine which isolates were capable of growing on specific individual HMOs as the sole carbon source.
Methods Stool sample collection Stool samples were regularly collected from nappies/diapers of preterm infants into sterile collection pots by nurse staff. Samples were initially stored at -20°C before being transferred to -80°C for long term storage.
Bacterial isolation and identification Stool samples were thawed on ice and initially diluted roughly 1:10 w/v in sterile anaerobic PBS. 10-fold serial dilutions were then performed using sterile anaerobic PBS and various dilutions (typically 10-2 and 10-4) were cultured by adding 100p1 of inoculum onto an agar plate, before spreading to cover the surface of the agar plate and incubating for up to 96 hours.
Numerous different agar media was used including brain heart infusion (BHI), Transoligosaccharide propionate (TOS), Bifidus Selective Medium (BSM), De Man, Rogosa and Sharpe (MRS), fastidious anaerobe agar (FAA), and yeast extract-peptone-dextrose (YPD). Unique appearing colonies based on morphology, colour, and size were sub-cultured twice before undergoing full-length 16S sequencing and/or MALDI-TOF to identify the isolate to genus or species level. Isolates were added to glycerol for long term storage.
HMO growth curves Glycerol stocks were part-thawed and transferred to 5m1 broth based on the isolate's preferred medium. Zhang Mills Block 1 (ZMB1) without glucose was prepared, and various carbon sources were tested in 1% concentration. Controls consisted of no carbon source, glucose, and lactose. Tested HMOs were 2'FL, DSLNT, LNT, LNnT, LNFP I, 6SL. The overnight growth for each isolate was centrifuged at 5000g for 5 mins at 4°C, and the pellet was resuspended in the same amount of anaerobic PBS. 20p1 of the bacteria in PBS was aliquoted into 180p1 of media, and the growth was measured in a Cerillo Stratus plate reader for 150 hours. All isolates were tested in triplicate, and wells with media only were included in each plate to check for contamination.
Results Only Clostridium species and Bifidobacterium species were able to grow on HMOs. Within Clostridium, the following species were found to use at least one HMO, which has not previously been reported: C. perfringens, C. baratii, and C. tedium (Fig. 1). Within Bifidobacterium, the following species were found to use at least one HMO, which have all been reported previously: B. infantis, B. bifidum, B. breve, and B. longum (Fig. 1). The dendrogram shows that 3 strains of C. perfringens and the only C. tedium cluster closely with a B. infantis strain. Only one of these strains, a C. perfringens, was tested with DLSNT, showing it was able to use this HMO. Notably, due to limited DLSNT, not all strains of Clostridium could be tested for growth on this HMO. Of all Bifidobacterium strains tested on DLSNT, only B. bifidum showed growth, and this was comparably lower than C. perfringens.
Of all Bifidobacterium strains, only B. animalis was not tested on DLSNT and it is unlikely this strain can use this HMO given it could not use any other HMO or lactose for growth. Aside from DLSNT, all C. perfringens, C. baratii, and C. tedium were able to use LNnT. C. baratii, C. tedium and one other strain of C. perfringens could use LNT. Most C. perfringens strains could use 6SL (6/7) and 2FL (4/7). Only one stain of C. perfringens, one strain of B. infantis, and one strain of B. bifidum could use LNFP I. When growing on HMOs, HMO-utilising bacteria produce short chain fatty acids, convert aromatic amino acids into aromatic lactic acids (e.g., indolelactic acid; ILA), and potentially release other functionally important compounds that have important immunomodulatory roles in host health. (Laursen et al. Nat Microbic!. 2021; Henrick et al. Cell. 2021 Jul 22;184(15)). For instance, ILA has been shown to limit T cell activation and reduce a pro-inflammatory response by inducing galectin-1 in TH2 and TH17 cells during helper T cell polarisation (Henrick et al. Cell. 2021 Jul 22;184(15)). In terms of short chain fatty acid production, Clostridium are able to produce butyrate when from dietary glycans and higher butyrate has previously been linked to reduced NEC risk. Thus, HMO-utilising bacteria are widely considered probiotic bacteria and higher levels of HMO-utilising bacteria in the gut has been associated with reduced inflammation and improved health. Furthermore, higher levels of DLSNT in mothers own milk is linked to reduced risk of NEC, potentially relating to modification of the infant gut microbiome and promotion of bacteria that can utilise this HMO. The disclosure provides a method for increasing Clostridium abundance in the gut, including strains that are able to use a range of HMOs including DSLNT.
Example II
Experimental Design To identify if HMO-utilising bacteria found in the preterm infant gut microbiome, namely Clostridium species and Bifidobacterium species, are associated with protection from NEC, preterm infants diagnosed with NEC provided longitudinal stool samples spanning the disease course. To identify bacteria enriched in non-NEC preterm infants, healthy controls who did not develop NEC also provided longitudinal stool samples.
Methods Stool sample collection As previously explained, stool samples were regularly collected from nappies/diapers of preterm infants into sterile collection pots by nurse staff. Samples were initially stored at -20°C before being transferred to -80°C for long term storage.
Metagenomic sequencing DNA was extracted from 100mg of stool sample using the DNeasy PowerSoil Kit (QIAGEN).
Library prep was performed using the Nextera DNA Flex Kit. Sequencing was performed on the HiSeq X Ten (Illumina) with a target read depth of 10M reads per sample with a read length of 150bp paired end reads. Taxonomic profiling of metagenomic samples was performed using MetaPhlAn v.2.0. All samples containing <100,000 were removed from the analysis.
Analysis of metagenomic dataset The relative abundance of the six most abundant Bifidobacterium species and six most abundant Clostridium species were first plotted over the first 150 days of life, faceted by NEC or healthy status. Correlations were visually inspected, with particular focus on the critical period of NEC risk between days 10 and 40 when most NEC is diagnosed. Generalized linear mixed models (GLMMs) were fit to the data using the glmmTMB package v.1.0.2.1 in R. The general formula for each of the LMMs fitted was as follows: Y = X1 + X2 + + X" + (11SubjectID).
To find out which co-variates were significantly associated with Clostridium perfringens relative abundance, mixed-effects models were fit using the Gaussian distribution. NEC status, breastmilk status, and birth mode were included as fixed effects and subject ID was included as a random group intercept.
Results Clostridium perfringens was elevated early in life in healthy (no-NEC) preterm infants in comparison to preterm infants diagnosed with NEC (Fig. 2). This was especially apparent over the critical period of NEC risk between days 10 and 40 when most NEC is diagnosed (Fig. 2).
In contrast, the six most abundant Bifidobacterium species showed relatively similar trajectories between healthy and NEC diagnosed preterm infants (Fig. 3). GLMMs of Clostridium perfringens before (preNEC) and after (postNEC) NEC diagnosis compared to healthy preterm infants as the reference group show a significant increase in C. perfringens in healthy preterm infants prior to disease (Fig. 4). No difference was shown in C. perfringens relative abundance in preterm infants after they recover from NEC compared to healthy preterm infants (Fig. 4). This disclosure provides a method of increasing Clostridium abundance, including but not limited to Clostridium perfringens, in the (preterm) gut to reduce the risk of NEC and other diseases.
Example Ill Methods
Stool sample collection As previously explained, stool samples were regularly collected from nappies/diapers of preterm infants into sterile collection pots by nurse staff. Samples were initially stored at -20°C before being transferred to -80°C for long term storage.
Preparation of postbiotic A loopful of a glycerol stock was streaked out across an agar plate of the isolate's preferred medium and incubated anaerobically at 37°C overnight. A single colony transferred to 5m1 broth based on the isolate's preferred medium and incubated anaerobically at 37°C overnight. Two 5 mL aliquots of ZMB1 were prepared. Glucose (5 mg/ml) was added one aliquot, while the HMOs (5 mg/ml of each) known to sustain growth of the isolate were added to the second aliquot. The OD600 of the overnight growth of the isolate was measured and the culture diluted to OD 1. Two 250 pL aliquots of the culture were then centrifuged at 5000g for 5 mins at 4°C, and the pellet was resuspended in the same amount of anaerobic PBS. Centrifugation was repeated and the pellets then resuspended in 250 pL of 'ZMB1 with glucose' or 'ZMB1 with HMOs'. The culture aliquots were then added to the remaining 4.75 mL of 2MB1 with glucose' or 2MB1 with HMOs'. The two cultures were then incubated anaerobically, at 37°C, with shaking at 130 rpm, for 6 days. At the end of the incubation period, the two cultures were centrifuged at 5000g for 5 mins at 4°C, and the supernatants transferred to new tubes in the anaerobic chamber. These supernatants were then filter sterilised with Merck MillexTm-GP Sterile 0.22 pm syringe filters.
ZMB1 is described in detail in Zhang et al Appl Env iron Microbic!. 2009 Feb 75(4): 1080- 1087. Other appropriate media may also be used.
Assay to determine inhibitory potential of postbiotic on different pathobionts Glycerol stocks of pathobionts were streaked out on agar plates of each isolate's preferred medium and incubated anaerobically at 37°C overnight. Single colonies were transferred to 5m1 broth based on each isolate's preferred medium and incubated anaerobically at 37°C overnight. On the same day, a fresh batch of ZMB1 medium was prepared and split into 3. One of the ZMB1 aliquots was supplemented with glucose (20 mg/ml), another supplemented with the same HMOs (5 mg/ml of each) used in the postbiotic, and the third aliquot was left without a carbon source. All media aliquots were then left in the anaerobic chamber overnight to deplete oxygen. The next day, the OD600 of each pathobiont culture was measured and volumes required to dilute each to an OD600 of 0.5 in 1.5 mL ZMB1 were calculated. The required volumes of the cultures were then centrifuged at 5000g for 5 mins at 4°C and the supernatants removed. The pellets were then resuspended in 1 mL anaerobic PBS and centrifuged again as before, with the supernatants removed. All pellets were then resuspended in 1.5 mL ZMB1 with glucose. 100 uL of each pathobiont was then added to either 1) 100 uL of ZMB1 with HMOs postbiotic, 2) 100 uL of ZMB1 with glucose postbiotic, 3) 100 uL of sterile ZMB1 with HMOs medium, and 4) 100 uL of sterile ZMB1 medium (no carbon source). All experiments were performed in triplicate. 200 uL of each media condition used (ZMB1, 'ZMB1 with glucose', 'ZMB1 with HMOs' and the two postbiotics) was added to 5 separate wells to act as blanks and to check for contamination during the assay. The plate was then shaken at 100 rpm for 1 minute. The lid was then removed and the plate sealed with a Diversified Biotech Breathe-Easy plate seal. The sealed plate was then placed in a Cerillo Stratus plate reader and the assay run for 24 hours, with readings taken at 600 nm.
Results Growth of all four pathobionts tested (E. colt, E. faecalis, K. oxytoca and K. pneumoniae) was significantly inhibited by the C. perfringens derived HMO postbiotic during the first 10 hours of incubation, compared to the regular medium control (Fig. 5). This effect was not due to the presence of unmetabolized HMOs, as shown by the 'HMO control' condition having produced similar AUCs to the regular medium control and higher AUCs compared to the HMO postbiotic. For all pathobionts tested with the exception E. faecal's, the glucose postbiotic also inhibited growth compared to controls, demonstrating that for three of the strains, the inhibitory effect was not solely dependent on HMO metabolism by the probiotic strain. However, for all four pathobionts, the HMO postbiotic showed significantly enhanced inhibition compared to the glucose postbiotic. These results suggest that C. perfringens can utilise HMOs to produce antibacterial effectors.
The reader's attention is directed to all papers and documents which are filed concurrently with or previous to this specification in connection with this application and which are open to public inspection with this specification, and the contents of all such papers and documents are incorporated herein by reference.
All of the features disclosed in this specification (including any accompanying claims, abstract and drawings), and/or all of the steps of any method or process so disclosed, may be combined in any combination, except combinations where at least some of such features and/or steps are mutually exclusive.
Each feature disclosed in this specification (including any accompanying claims, abstract and drawings), may be replaced by alternative features serving the same, equivalent, or similar purpose, unless expressly stated otherwise. Thus, unless expressly stated otherwise, each feature disclosed is one example only of a generic series of equivalent or similar features.
The invention is not restricted to the details of any foregoing embodiments. The invention extends to any novel one, or any novel combination, of the features disclosed in this specification (including any accompanying claims, abstract and drawings), or to any novel one, or any novel combination, of the steps of any method or process so disclosed.
Claims (28)
- Claims 1. A probiotic composition for improving the gut microbiome and/or gut health of a subject, the composition comprising at least one Clostridium species selected from the group consisting of: Clostridium perfringens, Clostridium baratii and Clostridium tedium.
- 2. The probiotic composition according to claim 1, wherein the composition comprises at least two Clostridium species selected from the group consisting of: Clostridium perfringens, Clostridium baratii and Clostridium tedium.
- 3. The probiotic composition according to any preceding claim, wherein the composition comprises at least 0.5 x 109 CFU Clostridium species per dose.
- 4. The probiotic composition according to any preceding claim, further comprising at least one prebiotic.
- 5. The probiotic composition according to claim 4, wherein the prebiotic is a mammalian milk oligosaccharide (MMO), a precursor and/or a derivative thereof
- 6. The probiotic composition according to claim 5, wherein the MMO is a human milk oligosaccharide (HMO) a precursor and/or a derivative thereof.
- 7. The probiotic composition according to claim 6, wherein the HMO is selected from the group consisting of: lacto-N-tetraose (LNT), lacto-N-neotetraose (LNnT), disialyllacto-Ntetraose (DSLNT), lacto-N-fucopentaose I (LNFPI), 6'-sialyllactose (6SL) and 2-fucosyllactose (2FL); or a combination thereof; optionally wherein the HMO is LNnT and/or 6SL.
- 8. A postbiotic composition for improving the gut health of a subject, the composition comprising: (a) at least one inanimate Clostridium species selected from the group consisting of: Clostridium perfringens, Clostridium baratii and Clostridium tedium; and/or (b) metabolites from at least one Clostridium species selected from the group consisting of: Clostridium perfringens, Clostridium baratii and Clostridium tedium.
- 9. The postbiotic composition of claim 8, wherein the composition comprises: (a) at least two inanimate Clostridium species selected from the group consisting of: Clostridium perfringens, Clostridium baratii and Clostridium tedium; and/or (b) metabolites from at least two Clostridium species selected from the group consisting of: Clostridium perfringens, Clostridium baratii and Clostridium tedium.
- 10. The probiotic composition or postbiotic composition according to any one of the preceding claims, wherein the composition is in the form of a liquid medium, gel medium, powder, dissolvable medium, chewable medium, or any other ingestible medium.
- 11. The probiotic composition or postbiotic composition according to claim 10, wherein the composition is a milk product.
- 12. The probiotic composition or postbiotic composition according to claim 11, wherein the composition is an infant formula, a starter infant formula, a follow-on or follow-up infant formula, a baby food, or an infant cereal composition.
- 13. The probiotic composition or postbiotic composition according to any one of claims 10 to 12, wherein the composition is a powder, a liquid or a solid.
- 14. A probiotic composition or postbiotic composition of any one of claims 1 to 13 for use in improving the gut microbiome and/or the gut health of a subject.
- 15. The probiotic composition for use, or postbiotic composition for use, according to claim 14, wherein the subject is an infant.
- 16. The probiotic composition for use, or postbiotic composition for use, according to claim 15, wherein the infant is a neonate, a preterm infant, an infant small for gestational age, or an infant born by C-section.
- 17. The probiotic composition for use, or postbiotic composition for use, according to claim or claim 16, wherein the infant is hospitalised and/or is undergoing antibiotic treatment.
- 18. The probiotic composition for use, or postbiotic composition for use, according to any one of claims 13 to 17, wherein the composition reduces pathogen overgrowth in the gut, promotes gut development, promotes gut maturation, promotes immunity, decreases gut inflammation, and/or promotes enteral feeding tolerance in the subject.
- 19. A probiotic composition or postbiotic composition of any one of claims 1 to 13 for use in preventing or treating a gastrointestinal disorder, an allergy, nappy rash, colic, or a symptom thereof, in a subject.
- 20. A method of treating or preventing a gastrointestinal disorder, an allergy, nappy rash, colic, or a symptom thereof in a subject, comprising administering a therapeutically effective amount of the probiotic composition or the postbiotic composition of any one of claims 1 to 13 to said subject.
- 21. The probiotic composition for use, or postbiotic composition for use, according to claim 19, or the method according to claim 20, wherein the gastrointestinal disorder affects the integrity of the intestinal lining.
- 22. The probiotic composition for use, or postbiotic composition for use according to claim 19 or 21, or the method according to claim 20 to 21, wherein the gastrointestinal disorder is selected from the group consisting of: intestinal inflammation, irritable bowel syndrome, colitis, necrotising enterocolitis, late onset sepsis, focal intestinal perforation, and inflammatory bowel disease.
- 23. The probiotic composition for use, or postbiotic composition for use, according claim 22, or the method according to claim 22, wherein the gastrointestinal disorder is necrotising enterocolitis.
- 24. The probiotic composition for use, or postbiotic composition for use, according to any one of claims19, 21 to 23, or the method according to any one of claims 20 to 23, wherein the subject is an infant.
- 25. The probiotic composition for use, or postbiotic composition for use, or the method, according to claim 24, wherein the infant is a neonate, a preterm infant, an infant small for gestational age, or an infant born by C-section.
- 26. The probiotic composition for use, or postbiotic for use, or the method, according to claim 24 or claim 25, wherein the infant is hospitalised and/or is undergoing antibiotic treatment.
- 27. Use of Clostridium perfringens, Clostridium baratii and/or Clostridium tedium as a probiotic or postbiotic.
- 28. Clostridium petfringens, Clostridium baratii and/or Clostridium tettium, or a Clostridium postbiotic thereof, for use in improving the gut microbiome and/or gut health in a subject.
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WO2002007741A1 (en) * | 2000-07-25 | 2002-01-31 | Borody Thomas J | Probiotic recolonisation therapy |
US20180369297A1 (en) * | 2015-12-14 | 2018-12-27 | Metabogen Ab | Treatment of intrahepatic cholestasis and related liver diseases |
CN109275911A (en) * | 2018-11-02 | 2019-01-29 | 梁余成 | A kind of feeding probiotic tablet and preparation method thereof |
US20190350988A1 (en) * | 2012-11-23 | 2019-11-21 | Seres Therapeutics, Inc. | Synergistic Bacterial Compositions and Methods of Production and Use Thereof |
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US8197872B2 (en) | 2007-05-17 | 2012-06-12 | The Regents Of The University Of California | Human milk oligosaccharides to promote growth of beneficial gut bacteria |
EP2405918B2 (en) | 2009-03-13 | 2020-09-02 | The Regents of The University of California | Prebiotic oligosaccharides |
ES2680920T3 (en) | 2010-07-12 | 2018-09-11 | The Regents Of The University Of California | Bovine milk oligosaccharides |
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WO2002007741A1 (en) * | 2000-07-25 | 2002-01-31 | Borody Thomas J | Probiotic recolonisation therapy |
US20190350988A1 (en) * | 2012-11-23 | 2019-11-21 | Seres Therapeutics, Inc. | Synergistic Bacterial Compositions and Methods of Production and Use Thereof |
US20180369297A1 (en) * | 2015-12-14 | 2018-12-27 | Metabogen Ab | Treatment of intrahepatic cholestasis and related liver diseases |
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