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GB2421029B - Method of in vitro differentiation of neural stem cells, motor neurons and dopamine neurons from primate embryonic stem cells - Google Patents

Method of in vitro differentiation of neural stem cells, motor neurons and dopamine neurons from primate embryonic stem cells

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Publication number
GB2421029B
GB2421029B GB0605851A GB0605851A GB2421029B GB 2421029 B GB2421029 B GB 2421029B GB 0605851 A GB0605851 A GB 0605851A GB 0605851 A GB0605851 A GB 0605851A GB 2421029 B GB2421029 B GB 2421029B
Authority
GB
United Kingdom
Prior art keywords
stem cells
neurons
vitro differentiation
primate embryonic
embryonic stem
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
GB0605851A
Other versions
GB0605851D0 (en
GB2421029A (en
Inventor
Su-Chun Zhang
James A Thomson
Ian David Duncan
Xue-Jun Li
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wisconsin Alumni Research Foundation
Original Assignee
Wisconsin Alumni Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wisconsin Alumni Research Foundation filed Critical Wisconsin Alumni Research Foundation
Publication of GB0605851D0 publication Critical patent/GB0605851D0/en
Publication of GB2421029A publication Critical patent/GB2421029A/en
Application granted granted Critical
Publication of GB2421029B publication Critical patent/GB2421029B/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A61P25/16—Anti-Parkinson drugs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0623—Stem cells
    • C—CHEMISTRY; METALLURGY
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/44—Thiols, e.g. mercaptoethanol
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/46—Amines, e.g. putrescine
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/119—Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/13—Nerve growth factor [NGF]; Brain-derived neurotrophic factor [BDNF]; Cilliary neurotrophic factor [CNTF]; Glial-derived neurotrophic factor [GDNF]; Neurotrophins [NT]; Neuregulins
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/41—Hedgehog proteins; Cyclopamine (inhibitor)
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/90—Polysaccharides
    • C12N2501/91—Heparin
    • C—CHEMISTRY; METALLURGY
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Reproductive Health (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gynecology & Obstetrics (AREA)
  • Psychology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
GB0605851A 2003-08-29 2004-08-27 Method of in vitro differentiation of neural stem cells, motor neurons and dopamine neurons from primate embryonic stem cells Expired - Lifetime GB2421029B (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US49883103P 2003-08-29 2003-08-29
US49957003P 2003-09-02 2003-09-02
PCT/US2004/027841 WO2005021720A2 (en) 2003-08-29 2004-08-27 Method of in vitro differentiation of neural stem cells, motor neurons and dopamine neurons from primate embryonic stem cells

Publications (3)

Publication Number Publication Date
GB0605851D0 GB0605851D0 (en) 2006-05-03
GB2421029A GB2421029A (en) 2006-06-14
GB2421029B true GB2421029B (en) 2008-04-09

Family

ID=34278616

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GB0605851A Expired - Lifetime GB2421029B (en) 2003-08-29 2004-08-27 Method of in vitro differentiation of neural stem cells, motor neurons and dopamine neurons from primate embryonic stem cells

Country Status (8)

Country Link
EP (1) EP1670901A4 (en)
JP (2) JP2007503811A (en)
KR (2) KR20060115351A (en)
AU (1) AU2004269361B2 (en)
CA (1) CA2536588C (en)
GB (1) GB2421029B (en)
IL (2) IL173832A (en)
WO (1) WO2005021720A2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8153424B2 (en) 2001-10-03 2012-04-10 Wisconsin Alumni Research Foundation Method of in vitro differentiation of neural stem cells, motor neurons and dopamine neurons from primate embryonic stem cells
US9080151B2 (en) 2001-10-03 2015-07-14 Wisconsin Alumni Research Foundation Method of in vitro differentiation of neural stem cells, motor neurons and dopamine neurons from primate embryonic stem cells

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US7632679B2 (en) 2002-07-16 2009-12-15 The Trustees Of Columbia University In The City Of New York Systems and methods for screening for modulators of neural differentiation
JP4792290B2 (en) * 2005-12-27 2011-10-12 財団法人 東京都医学総合研究所 Method for producing neural stem cells
MY154677A (en) 2006-03-07 2015-07-15 Shroff Geeta Compositions comprising human embryonic stem cells and their derivatives, methods of use, and methods of preparation
KR100838013B1 (en) * 2006-06-07 2008-06-12 제일약품주식회사 Efficient Generation of Neural Progenitors, Neurons and Dopaminergic Neurons from Human Embryonic Stem Cells
EP2279247B1 (en) * 2008-04-22 2019-01-02 Regenerative Research Foundation Retinal pigment epithelial stem cells
KR101168053B1 (en) * 2009-11-06 2012-07-24 연세대학교 산학협력단 Efficient and Universal Method for Neural Differentiation of Pluripotent Stem Cells
CA2805773A1 (en) 2010-07-30 2012-02-02 Cambridge Enterprise Limited Corticogenesis of human pluripotent cells
EP2694644B1 (en) 2011-03-30 2018-01-24 Cellular Dynamics International, Inc. Priming of pluripotent stem cells for neural differentiation
JP6015112B2 (en) * 2012-05-11 2016-10-26 株式会社ニコン Cell evaluation apparatus, cell evaluation method and program
CN108291226B (en) * 2015-11-12 2022-11-04 豪夫迈·罗氏有限公司 Oligonucleotides for inducing paternal UBE3A expression
GB2569058B (en) 2016-01-12 2021-04-14 Cedars Sinai Medical Center A method of osteogenic differentiation in microfluidic tissue culture systems
WO2017136479A1 (en) 2016-02-01 2017-08-10 Cedars-Sinai Medical Center Systems and methods for growth of intestinal cells in microfluidic devices
WO2017209290A1 (en) * 2016-06-03 2017-12-07 国立大学法人京都大学 Production and use of motor neuron derived from pluripotent stem cell
JP6376196B2 (en) * 2016-09-29 2018-08-22 株式会社ニコン Cell evaluation apparatus, cell evaluation method and program
US11913022B2 (en) 2017-01-25 2024-02-27 Cedars-Sinai Medical Center In vitro induction of mammary-like differentiation from human pluripotent stem cells
US11767513B2 (en) 2017-03-14 2023-09-26 Cedars-Sinai Medical Center Neuromuscular junction
WO2018176001A2 (en) 2017-03-24 2018-09-27 Cedars-Sinai Medical Center Methods and compositions for production of fallopian tube epithelium
CN108048399B (en) * 2017-11-13 2021-03-23 昆明医科大学 Method for inducing tree shrew dopamine neuron primary cell autophagy by METH
JP2019106895A (en) * 2017-12-15 2019-07-04 インダストリー‐ユニバーシティー コーぺレーション ファンデーション ハンヤン ユニバーシティ Method of differentiating neural stem cells or neural precursor cells into dopamine neurons
EP3775161A4 (en) * 2018-04-06 2022-04-06 Cedars-Sinai Medical Center Human pluripotent stem cell derived neurodegenerative disease models on a microfluidic chip
WO2019195800A1 (en) 2018-04-06 2019-10-10 Cedars-Sinai Medical Center Novel differentiation technique to generate dopaminergic neurons from induced pluripotent stem cells
JP2021532736A (en) * 2018-06-06 2021-12-02 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニアThe Regents Of The University Of California Neural stem cell composition and methods for treating neurodegenerative disorders
JP6798533B2 (en) * 2018-07-26 2020-12-09 株式会社ニコン Cell evaluation device, incubator and program
CN114375328A (en) * 2019-09-06 2022-04-19 学校法人庆应义塾 Method for producing cell aggregate containing glial precursor cell
JP7235027B2 (en) * 2020-11-16 2023-03-08 株式会社ニコン Cell evaluation instruments, incubators and programs

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WO2001083715A2 (en) * 2000-05-01 2001-11-08 THE GOVERNMENT OF THE UNITED STATES OF AMERICA as represented by the Secretary, Derivation of midbrain dopaminergic neurons from embryonic stem cells
US20020009743A1 (en) * 2000-05-17 2002-01-24 Carpenter Melissa K. Neural progenitor cell populations
US20020168763A1 (en) * 2000-11-30 2002-11-14 Yan Wen Liang Isolated homozygous stem cells, differentiated cells derived therefrom, and materials and methods for making and using same
US20030211605A1 (en) * 2001-05-01 2003-11-13 Lee Sang-Hun Derivation of midbrain dopaminergic neurons from embryonic stem cells

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AU743251B2 (en) * 1997-08-04 2002-01-24 Regents Of The University Of California, The Methods for treating neurological deficits
US6887706B2 (en) * 2001-10-03 2005-05-03 Wisconsin Alumni Research Foundation Method of in vitro differentiation of transplantable neural precursor cells from primate embryonic stem cells

Patent Citations (4)

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WO2001083715A2 (en) * 2000-05-01 2001-11-08 THE GOVERNMENT OF THE UNITED STATES OF AMERICA as represented by the Secretary, Derivation of midbrain dopaminergic neurons from embryonic stem cells
US20020009743A1 (en) * 2000-05-17 2002-01-24 Carpenter Melissa K. Neural progenitor cell populations
US20020168763A1 (en) * 2000-11-30 2002-11-14 Yan Wen Liang Isolated homozygous stem cells, differentiated cells derived therefrom, and materials and methods for making and using same
US20030211605A1 (en) * 2001-05-01 2003-11-13 Lee Sang-Hun Derivation of midbrain dopaminergic neurons from embryonic stem cells

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8153424B2 (en) 2001-10-03 2012-04-10 Wisconsin Alumni Research Foundation Method of in vitro differentiation of neural stem cells, motor neurons and dopamine neurons from primate embryonic stem cells
US9080151B2 (en) 2001-10-03 2015-07-14 Wisconsin Alumni Research Foundation Method of in vitro differentiation of neural stem cells, motor neurons and dopamine neurons from primate embryonic stem cells

Also Published As

Publication number Publication date
IL173832A0 (en) 2006-07-05
WO2005021720A2 (en) 2005-03-10
IL198450A (en) 2011-01-31
JP2007503811A (en) 2007-03-01
WO2005021720A3 (en) 2005-05-19
KR20060115351A (en) 2006-11-08
AU2004269361A1 (en) 2005-03-10
JP2010162024A (en) 2010-07-29
EP1670901A4 (en) 2007-09-05
AU2004269361B2 (en) 2010-05-20
GB0605851D0 (en) 2006-05-03
CA2536588A1 (en) 2005-03-10
JP5529561B2 (en) 2014-06-25
IL173832A (en) 2011-02-28
GB2421029A (en) 2006-06-14
KR20080109943A (en) 2008-12-17
CA2536588C (en) 2018-04-24
EP1670901A2 (en) 2006-06-21

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Expiry date: 20240826