GB2480419A - Yeast for fructan utilisation - Google Patents
Yeast for fructan utilisation Download PDFInfo
- Publication number
- GB2480419A GB2480419A GB1116293A GB201116293A GB2480419A GB 2480419 A GB2480419 A GB 2480419A GB 1116293 A GB1116293 A GB 1116293A GB 201116293 A GB201116293 A GB 201116293A GB 2480419 A GB2480419 A GB 2480419A
- Authority
- GB
- United Kingdom
- Prior art keywords
- yeast
- yeast cell
- fructan
- utilisation
- fructans
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 229920002670 Fructan Polymers 0.000 title abstract 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 title description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 108090000623 proteins and genes Proteins 0.000 abstract description 2
- 210000005253 yeast cell Anatomy 0.000 abstract 5
- ZFTFOHBYVDOAMH-XNOIKFDKSA-N (2r,3s,4s,5r)-5-[[(2r,3s,4s,5r)-5-[[(2r,3s,4s,5r)-3,4-dihydroxy-2,5-bis(hydroxymethyl)oxolan-2-yl]oxymethyl]-3,4-dihydroxy-2-(hydroxymethyl)oxolan-2-yl]oxymethyl]-2-(hydroxymethyl)oxolane-2,3,4-triol Chemical class O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@@H]1[C@@H](O)[C@H](O)[C@](CO)(OC[C@@H]2[C@H]([C@H](O)[C@@](O)(CO)O2)O)O1 ZFTFOHBYVDOAMH-XNOIKFDKSA-N 0.000 abstract 2
- 230000000694 effects Effects 0.000 abstract 2
- 108010005131 levanase Proteins 0.000 abstract 2
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 abstract 1
- 229930091371 Fructose Natural products 0.000 abstract 1
- 239000005715 Fructose Substances 0.000 abstract 1
- 108700005078 Synthetic Genes Proteins 0.000 abstract 1
- 230000001925 catabolic effect Effects 0.000 abstract 1
- 230000002503 metabolic effect Effects 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 8
- 229920001202 Inulin Polymers 0.000 description 2
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- JYJIGFIDKWBXDU-MNNPPOADSA-N inulin Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@]1(OC[C@]2(OC[C@]3(OC[C@]4(OC[C@]5(OC[C@]6(OC[C@]7(OC[C@]8(OC[C@]9(OC[C@]%10(OC[C@]%11(OC[C@]%12(OC[C@]%13(OC[C@]%14(OC[C@]%15(OC[C@]%16(OC[C@]%17(OC[C@]%18(OC[C@]%19(OC[C@]%20(OC[C@]%21(OC[C@]%22(OC[C@]%23(OC[C@]%24(OC[C@]%25(OC[C@]%26(OC[C@]%27(OC[C@]%28(OC[C@]%29(OC[C@]%30(OC[C@]%31(OC[C@]%32(OC[C@]%33(OC[C@]%34(OC[C@]%35(OC[C@]%36(O[C@@H]%37[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O%37)O)[C@H]([C@H](O)[C@@H](CO)O%36)O)[C@H]([C@H](O)[C@@H](CO)O%35)O)[C@H]([C@H](O)[C@@H](CO)O%34)O)[C@H]([C@H](O)[C@@H](CO)O%33)O)[C@H]([C@H](O)[C@@H](CO)O%32)O)[C@H]([C@H](O)[C@@H](CO)O%31)O)[C@H]([C@H](O)[C@@H](CO)O%30)O)[C@H]([C@H](O)[C@@H](CO)O%29)O)[C@H]([C@H](O)[C@@H](CO)O%28)O)[C@H]([C@H](O)[C@@H](CO)O%27)O)[C@H]([C@H](O)[C@@H](CO)O%26)O)[C@H]([C@H](O)[C@@H](CO)O%25)O)[C@H]([C@H](O)[C@@H](CO)O%24)O)[C@H]([C@H](O)[C@@H](CO)O%23)O)[C@H]([C@H](O)[C@@H](CO)O%22)O)[C@H]([C@H](O)[C@@H](CO)O%21)O)[C@H]([C@H](O)[C@@H](CO)O%20)O)[C@H]([C@H](O)[C@@H](CO)O%19)O)[C@H]([C@H](O)[C@@H](CO)O%18)O)[C@H]([C@H](O)[C@@H](CO)O%17)O)[C@H]([C@H](O)[C@@H](CO)O%16)O)[C@H]([C@H](O)[C@@H](CO)O%15)O)[C@H]([C@H](O)[C@@H](CO)O%14)O)[C@H]([C@H](O)[C@@H](CO)O%13)O)[C@H]([C@H](O)[C@@H](CO)O%12)O)[C@H]([C@H](O)[C@@H](CO)O%11)O)[C@H]([C@H](O)[C@@H](CO)O%10)O)[C@H]([C@H](O)[C@@H](CO)O9)O)[C@H]([C@H](O)[C@@H](CO)O8)O)[C@H]([C@H](O)[C@@H](CO)O7)O)[C@H]([C@H](O)[C@@H](CO)O6)O)[C@H]([C@H](O)[C@@H](CO)O5)O)[C@H]([C@H](O)[C@@H](CO)O4)O)[C@H]([C@H](O)[C@@H](CO)O3)O)[C@H]([C@H](O)[C@@H](CO)O2)O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JYJIGFIDKWBXDU-MNNPPOADSA-N 0.000 description 2
- 229940029339 inulin Drugs 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000228245 Aspergillus niger Species 0.000 description 1
- 101000641116 Bacillus sp. (strain L7) Levanase Proteins 0.000 description 1
- 240000003061 Bacillus sp. L7 Species 0.000 description 1
- 101001004086 Bacillus subtilis (strain 168) Levanbiose-producing levanase Proteins 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 240000008892 Helianthus tuberosus Species 0.000 description 1
- 235000003230 Helianthus tuberosus Nutrition 0.000 description 1
- 244000285963 Kluyveromyces fragilis Species 0.000 description 1
- 235000014663 Kluyveromyces fragilis Nutrition 0.000 description 1
- 241000186684 Lactobacillus pentosus Species 0.000 description 1
- 241000588902 Zymomonas mobilis Species 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 108010058100 fructan beta-fructosidase Proteins 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/0108—Fructan beta-fructosidase (3.2.1.80)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Botany (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The present invention relates to a yeast cell expressing one or more proteins with fructanase activity as well as a synthetic gene and an expression system for its expression in yeast cells. While naturally occurring yeast cells do not have any catabolic or metabolic capabilities to use fructans as a source, the yeast cell according to the invention has a fructanase activity and can break down or hydrolyse fructans into fructose molecules. The yeast cell of the present invention may be used in bioethanol production.
Description
GB 2480419 A continuation (56) cont FEMS microbiology letters, Vol. 154, 1997, AN Miasnikov, "Characterization of a novel endo-levanase and its gene from Bacillus sp.L7", pages 23 -28 Systematic and applied microbiology, Vol Paludan-MUller C. et al. "Purification and characterisation of an extracellular fructan beta-fructosidase from a Lactobacillus pentosus strain isolated from fermented fish", pages 13 -20 Applied biochemistry and biotechnology, Voll 29-132, 2006, Ma J Negro et al "Inulin-containing biomass for ethanol production: carbohydrate extraction and ethanol fermentation", pages 922 -932 Biotechnology letters Vol 26, 2004, K Szambelan et al. "Use of Zymomonas mobilis and Saccharomyces cerevisiae mixed with Kluyveromyces fragilis for improved ethanol production from Jerusalem artichoke tubers", pages 845 -848 Applied and environmental microbiology, Vol 59, 1993, K Ohta et al. "Production of high concentrations of ethanol from inulin by simultaneous saccharification and fermentation using Aspergillus niger and Saccharomyces cerevisiae". pages 729 -733.
(58) Field of Search by ISA:
INT CL CI2N, CI2R Other: ONLINE: EPO-INTERNAL, BIOSIS, CHEM ABS DATA, SEQUENCE SEARCH, EMBASE, WPI DATA
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US15558909P | 2009-02-26 | 2009-02-26 | |
| GB0903224A GB2468134A (en) | 2009-02-26 | 2009-02-26 | Yeast for fructan utilisation |
| PCT/EP2010/052350 WO2010097415A2 (en) | 2009-02-26 | 2010-02-24 | Yeast for fructan utilisation |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| GB201116293D0 GB201116293D0 (en) | 2011-11-02 |
| GB2480419A true GB2480419A (en) | 2011-11-16 |
| GB2480419B GB2480419B (en) | 2014-09-17 |
Family
ID=40565718
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB0903224A Withdrawn GB2468134A (en) | 2009-02-26 | 2009-02-26 | Yeast for fructan utilisation |
| GB1116293.0A Expired - Fee Related GB2480419B (en) | 2009-02-26 | 2010-02-24 | Method for the manufacture of ethanol |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB0903224A Withdrawn GB2468134A (en) | 2009-02-26 | 2009-02-26 | Yeast for fructan utilisation |
Country Status (2)
| Country | Link |
|---|---|
| GB (2) | GB2468134A (en) |
| WO (1) | WO2010097415A2 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2576762A4 (en) | 2010-06-03 | 2013-12-04 | Mascoma Corp | Yeast expressing saccharolytic enzymes for consolidated bioprocessing using starch and cellulose |
| CN115838710B (en) * | 2022-12-05 | 2024-06-18 | 江苏海洋大学 | Exo-levan enzyme capable of degrading levan and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2485339A1 (en) * | 1980-06-25 | 1981-12-31 | Beghin Say Sa | Prodn. of syrups contg. glucose and fructose - by contacting poly:fructoside(s) with microorganisms |
| AT398435B (en) * | 1992-08-20 | 1994-12-27 | Helmut Dipl Ing Dr Schwab | Modified polyfructan-hydrolyzing enzyme |
| WO2000068402A1 (en) * | 1999-05-06 | 2000-11-16 | K.U. Leuven Research & Development | Manipulation of fructan catabolism in plants |
| WO2010097416A1 (en) * | 2009-02-26 | 2010-09-02 | Uws Ventures Limited | Fructanase |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2161652C1 (en) * | 2000-02-08 | 2001-01-10 | Московский Государственный Университет пищевых производств | Method of production of ethyl alcohol from jerusalem artichoke |
| US7314974B2 (en) * | 2002-02-21 | 2008-01-01 | Monsanto Technology, Llc | Expression of microbial proteins in plants for production of plants with improved properties |
| JP4592387B2 (en) * | 2004-11-02 | 2010-12-01 | 株式会社廣貫堂 | Inulin-degrading enzyme and its gene |
| WO2008040358A1 (en) * | 2006-10-03 | 2008-04-10 | Protanol Bv | A method for providing proteins and fermentation products from a plant material |
-
2009
- 2009-02-26 GB GB0903224A patent/GB2468134A/en not_active Withdrawn
-
2010
- 2010-02-24 WO PCT/EP2010/052350 patent/WO2010097415A2/en active Application Filing
- 2010-02-24 GB GB1116293.0A patent/GB2480419B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2485339A1 (en) * | 1980-06-25 | 1981-12-31 | Beghin Say Sa | Prodn. of syrups contg. glucose and fructose - by contacting poly:fructoside(s) with microorganisms |
| AT398435B (en) * | 1992-08-20 | 1994-12-27 | Helmut Dipl Ing Dr Schwab | Modified polyfructan-hydrolyzing enzyme |
| WO2000068402A1 (en) * | 1999-05-06 | 2000-11-16 | K.U. Leuven Research & Development | Manipulation of fructan catabolism in plants |
| WO2010097416A1 (en) * | 2009-02-26 | 2010-09-02 | Uws Ventures Limited | Fructanase |
Non-Patent Citations (9)
Also Published As
| Publication number | Publication date |
|---|---|
| GB2480419B (en) | 2014-09-17 |
| GB201116293D0 (en) | 2011-11-02 |
| WO2010097415A3 (en) | 2010-12-02 |
| WO2010097415A2 (en) | 2010-09-02 |
| GB2468134A (en) | 2010-09-01 |
| GB0903224D0 (en) | 2009-04-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Wisselink et al. | Novel evolutionary engineering approach for accelerated utilization of glucose, xylose, and arabinose mixtures by engineered Saccharomyces cerevisiae strains | |
| Zhao et al. | Yeast flocculation: new story in fuel ethanol production | |
| van Rensburg et al. | The metabolic burden of cellulase expression by recombinant Saccharomyces cerevisiae Y294 in aerobic batch culture | |
| Favaro et al. | Using an efficient fermenting yeast enhances ethanol production from unfiltered wheat bran hydrolysates | |
| Deesuth et al. | High ethanol production under optimal aeration conditions and yeast composition in a very high gravity fermentation from sweet sorghum juice by Saccharomyces cerevisiae | |
| Davison et al. | Heterologous expression of cellulase genes in natural Saccharomyces cerevisiae strains | |
| BR112014012963B8 (en) | Process for producing ethanol and yeast cell | |
| MX355841B (en) | Recombinant beta-glucosidase variants for production of soluble sugars from cellulosic biomass. | |
| Alriksson et al. | Overexpression of Saccharomyces cerevisiae transcription factor and multidrug resistance genes conveys enhanced resistance to lignocellulose-derived fermentation inhibitors | |
| Kefale et al. | Bioethanol Production and Optimization test from Agricultural Waste: The case of wet coffee processing waste (pulp) | |
| Flores et al. | Simultaneous saccharification and fermentation of Agave tequilana fructans by Kluyveromyces marxianus yeasts for bioethanol and tequila production | |
| Wan et al. | Ethanol Production from Nondetoxified Dilute‐Acid Lignocellulosic Hydrolysate by Cocultures of Saccharomyces cerevisiae Y5 and Pichia stipitis CBS6054 | |
| Qiu et al. | Engineering the robustness of Saccharomyces cerevisiae by introducing bifunctional glutathione synthase gene | |
| Wang et al. | Comparison of process configurations for ethanol production from acid-and alkali-pretreated corncob by Saccharomyces cerevisiae strains with and without β-glucosidase expression | |
| Inokuma et al. | Improvement of xylose fermentation ability under heat and acid co-stress in Saccharomyces cerevisiae using genome shuffling technique | |
| Li et al. | Increasing extracellular cellulase activity of the recombinant Saccharomyces cerevisiae by engineering cell wall-related proteins for improved consolidated processing of carbon neutral lignocellulosic biomass | |
| Liu et al. | Lactose fermentation by engineered Saccharomyces cerevisiae capable of fermenting cellobiose | |
| Cagnin et al. | Comparing laboratory and industrial yeast platforms for the direct conversion of cellobiose into ethanol under simulated industrial conditions | |
| Zhang et al. | Improved γ-Linolenic acid production from cellulose in Mucor circinelloides via coexpression of cellobiohydrolase and delta-6 desaturase | |
| Murata et al. | Potentials of multi-stress tolerant yeasts, Saccharomyces cerevisiae and Pichia kudriavzevii for fuel ethanol production from industrial cassava wastes | |
| Kyriakou et al. | Improvement of stress multi-tolerance and bioethanol production by Saccharomyces cerevisiae immobilised on biochar: Monitoring transcription from defence-related genes | |
| Alvira et al. | Strategies of xylanase supplementation for an efficient saccharification and cofermentation process from pretreated wheat straw | |
| BR112021010778A2 (en) | MODULATION OF NADPH GENERATION BY RECOMBINANT YEAST HOST CELL DURING FERMENTATION | |
| GB2480419A (en) | Yeast for fructan utilisation | |
| Bu et al. | Enhanced biodegradation of sugar cane bagasse by coculture of Clostridium thermocellum and Thermoanaerobacterium aotearoense supplemented with CaCO3 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PCNP | Patent ceased through non-payment of renewal fee |
Effective date: 20210224 |