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GB2369828A - Nuclear transfer with selected donor cells - Google Patents

Nuclear transfer with selected donor cells

Info

Publication number
GB2369828A
GB2369828A GB0205915A GB0205915A GB2369828A GB 2369828 A GB2369828 A GB 2369828A GB 0205915 A GB0205915 A GB 0205915A GB 0205915 A GB0205915 A GB 0205915A GB 2369828 A GB2369828 A GB 2369828A
Authority
GB
United Kingdom
Prior art keywords
nuclear transfer
donor cells
selected donor
cells
provides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB0205915A
Other versions
GB2369828B (en
GB0205915D0 (en
Inventor
David Wells
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of GB0205915D0 publication Critical patent/GB0205915D0/en
Publication of GB2369828A publication Critical patent/GB2369828A/en
Application granted granted Critical
Publication of GB2369828B publication Critical patent/GB2369828B/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A61P25/16—Anti-Parkinson drugs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A61P35/02—Antineoplastic agents specific for leukemia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1323—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/99—Coculture with; Conditioned medium produced by genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/10—Conditioning of cells for in vitro fecondation or nuclear transfer

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Neurology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Diabetes (AREA)
  • General Engineering & Computer Science (AREA)
  • Neurosurgery (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Cardiology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Oncology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Psychology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Obesity (AREA)
  • Endocrinology (AREA)
  • Reproductive Health (AREA)
  • Biophysics (AREA)

Abstract

The present invention provides a method of nuclear transfer by selecting and segregating G1 cells from a donor cell population. This method is advantageous over the prior art as it provides certainty as to the stage of the cell cycle which the donor nuclei are in and allows for the production of cloned transgenic or non-transgenic embryonic cells, reconstituted embryos and whole animals for agricultural, pharmaceutical. nutraceutical and biomedical applications.
GB0205915A 1999-09-14 2000-09-14 Nuclear transfer with selected donor cells Expired - Fee Related GB2369828B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NZ337792A NZ337792A (en) 1999-09-14 1999-09-14 Nuclear transfer and use in cloning
PCT/NZ2000/000179 WO2001019182A1 (en) 1999-09-14 2000-09-14 Nuclear transfer with selected donor cells

Publications (3)

Publication Number Publication Date
GB0205915D0 GB0205915D0 (en) 2002-04-24
GB2369828A true GB2369828A (en) 2002-06-12
GB2369828B GB2369828B (en) 2004-12-01

Family

ID=19927507

Family Applications (1)

Application Number Title Priority Date Filing Date
GB0205915A Expired - Fee Related GB2369828B (en) 1999-09-14 2000-09-14 Nuclear transfer with selected donor cells

Country Status (8)

Country Link
JP (1) JP2004500038A (en)
CN (1) CN1424870A (en)
AR (1) AR032284A1 (en)
AU (1) AU784371B2 (en)
DE (1) DE10084987T1 (en)
GB (1) GB2369828B (en)
NZ (1) NZ337792A (en)
WO (1) WO2001019182A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7122177B2 (en) 2000-01-20 2006-10-17 Diabcell Pty Ltd. Preparation and xenotransplantation of porcine islets

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1578192A4 (en) * 2002-12-10 2006-03-29 Gtc Biotherapeutics Inc Method and system for utilizing somatic cell nuclear transfer embryos as cell donors for additional nuclear transfer
JP2006081542A (en) * 2004-08-18 2006-03-30 Institute Of Physical & Chemical Research Method for creating clone mammal
JP2010075099A (en) * 2008-09-26 2010-04-08 Japan Science & Technology Agency Method for screening mammal nuclear transplantation embryo, nonhuman mammal nuclear transplantation embryo, clone nonhuman mammal and screening kit
US10570418B2 (en) 2014-09-02 2020-02-25 The Regents Of The University Of California Methods and compositions for RNA-directed target DNA modification
CN105483216B (en) * 2015-11-30 2019-01-15 四川农业大学 A kind of method of rabbit CETP genetic test meat breeding
CN108624621B (en) * 2018-01-17 2019-04-12 中国科学院上海生命科学研究院 The preparation method of the somatic cell clone animal of non-human primates
EP4118188A1 (en) 2020-03-11 2023-01-18 Bit Bio Limited Method of generating hepatic cells
CN111793671A (en) * 2020-07-22 2020-10-20 中国农业大学 Optimized preparation method of chromosome suspension suitable for binary flow sorting of sheep chromosomes
CN112715479A (en) * 2021-01-06 2021-04-30 吉沙阿牛 Goat ecological cycle breeding method

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999046982A1 (en) * 1998-03-16 1999-09-23 Relag Pty Ltd Porcine nuclear transfer

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999046982A1 (en) * 1998-03-16 1999-09-23 Relag Pty Ltd Porcine nuclear transfer

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
Biol. Reprod., Vol.46, 1992, Collas, P. et al., "Influence of cell cycle stage of the donor...", pp.492-500. *
Biol. Reprod., Vol.49, 1993, Campbell, K. H. S. et al., "Nuclear-cytoplasmic interactions...", pp.933-942. *
Biol. Reprod., Vol.60, 1999, Boquest, A. C. et al., "Flow cytometric cell cycle analysis...", pp.1013-1019. *
Mol. Reprod. Dev., Vol.39, 1994, Otaegui, P. J. et al., "Transfer of nuclei from 8-cell stage...", pp.147-152. *
Mol. Reprod. Dev., Vol.40, 1995, Pinto-Correia, C. et al., "Factors involved in nuclear reprogramming...", pp.292-304. *
P.N.A.S., Vol.96, 1999, Wakayama, T. et al., "Mice cloned from embryonic stem cells", pp.14984-14989. *
Science, Vol.280, 1998, Cibelli, J. B. et al., "Cloned transgenic calves...", pp.1256-1258. *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7122177B2 (en) 2000-01-20 2006-10-17 Diabcell Pty Ltd. Preparation and xenotransplantation of porcine islets
US8142769B2 (en) 2000-01-20 2012-03-27 Diabcell Pty Ltd Preparation and xenotransplantation of porcine islets

Also Published As

Publication number Publication date
DE10084987T1 (en) 2003-04-17
NZ337792A (en) 2002-03-28
AU784371B2 (en) 2006-03-23
CN1424870A (en) 2003-06-18
GB2369828B (en) 2004-12-01
GB0205915D0 (en) 2002-04-24
JP2004500038A (en) 2004-01-08
AU7462400A (en) 2001-04-17
WO2001019182A1 (en) 2001-03-22
AR032284A1 (en) 2003-11-05

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Legal Events

Date Code Title Description
732E Amendments to the register in respect of changes of name or changes affecting rights (sect. 32/1977)
PCNP Patent ceased through non-payment of renewal fee

Effective date: 20070914