GB2022595A - beta -Galactosidase and the use thereof - Google Patents
beta -Galactosidase and the use thereofInfo
- Publication number
- GB2022595A GB2022595A GB7919738A GB7919738A GB2022595A GB 2022595 A GB2022595 A GB 2022595A GB 7919738 A GB7919738 A GB 7919738A GB 7919738 A GB7919738 A GB 7919738A GB 2022595 A GB2022595 A GB 2022595A
- Authority
- GB
- United Kingdom
- Prior art keywords
- beta
- galactosidase
- enzyme
- lactose
- species
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
- C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Dispersion Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
A beta -galactosidase comprises an enzyme having a thermal stability such that when in purified form it has an activity half life of at least 11 DIVIDED 2 hours at 55 DEG C, at least 1 hour at 60 DEG C and at least 10 minutes at 65 DEG C, as measured using ONPG as substrate, said enzyme being derived from a bacterium of species Bacillus stearothermophilus. There is also provided a process for the production of the said beta -galactosidase, comprising culturing in a medium containing appropriate nutrients a micro- organism of species B. stearothermophilus having characteristics similar to or the same as the strains identified as NCIB 11407-11413, and which contains the said beta -Galactosidase. Additionally there is provided a process for the hydrolysis of lactose to a product comprising glucose and galactose which comprises contacting lactose with the said beta -galactosidase; e.g. at a temperature of at least 55 DEG C, preferably at least 60 DEG C or especially about 65 DEG C.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB7919738A GB2022595B (en) | 1978-06-07 | 1979-06-06 | S-galactosidase and the use thereof |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB7826503 | 1978-06-07 | ||
GB7919738A GB2022595B (en) | 1978-06-07 | 1979-06-06 | S-galactosidase and the use thereof |
Publications (2)
Publication Number | Publication Date |
---|---|
GB2022595A true GB2022595A (en) | 1979-12-19 |
GB2022595B GB2022595B (en) | 1982-08-25 |
Family
ID=26267928
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB7919738A Expired GB2022595B (en) | 1978-06-07 | 1979-06-06 | S-galactosidase and the use thereof |
Country Status (1)
Country | Link |
---|---|
GB (1) | GB2022595B (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0046614A1 (en) * | 1980-08-22 | 1982-03-03 | Région Wallonne représentée par l'Exécutif Régional Wallon | Process for the immobilisation of globular microbial cells by means of adhesion to a solid support |
-
1979
- 1979-06-06 GB GB7919738A patent/GB2022595B/en not_active Expired
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0046614A1 (en) * | 1980-08-22 | 1982-03-03 | Région Wallonne représentée par l'Exécutif Régional Wallon | Process for the immobilisation of globular microbial cells by means of adhesion to a solid support |
Also Published As
Publication number | Publication date |
---|---|
GB2022595B (en) | 1982-08-25 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
732 | Registration of transactions, instruments or events in the register (sect. 32/1977) | ||
PCNP | Patent ceased through non-payment of renewal fee |