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GB2018778A - Vectors derived from lambda bacteriophage - Google Patents

Vectors derived from lambda bacteriophage

Info

Publication number
GB2018778A
GB2018778A GB7913033A GB7913033A GB2018778A GB 2018778 A GB2018778 A GB 2018778A GB 7913033 A GB7913033 A GB 7913033A GB 7913033 A GB7913033 A GB 7913033A GB 2018778 A GB2018778 A GB 2018778A
Authority
GB
United Kingdom
Prior art keywords
mutations
gene
site
endonuclease
genes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB7913033A
Other versions
GB2018778B (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut Pasteur de Lille
Original Assignee
Institut Pasteur de Lille
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut Pasteur de Lille filed Critical Institut Pasteur de Lille
Publication of GB2018778A publication Critical patent/GB2018778A/en
Application granted granted Critical
Publication of GB2018778B publication Critical patent/GB2018778B/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

A vector formed of a modified DNA of bacteriophage lambda , comprises in combination: - mutations, particularly in the Q and S genes, having the effect of preventing or retarding the expression of the late genes, particularly of regulating the production of proteins necessary for the encapsidation of this modified DNA in a bacterium notably E. coli, provided with suppressors of said mutations; - a DNA fragment of bacterial origin comprising a part at least of the Z gene of E. coli and a promotor of a bacterial operon, inserted in a non-essential part of the genome of the phage and a site of cleavage by an endonuclease, particularly EcoRI, in the above- said portion of the Z gene, to the exclusion of any other site of cleavage by the same endonuclease in the above-mentioned modified DNA. This vector enables the insertion in the site of said endonuclease of the gene of a determined protein, and the expression and production of a hybrid protein comprising said determined protein, by bacteria infected with said vector. The mutations in the Q and S genes delay the bacterial lysis and favor the accumulation of numerous copies of the hybrid protein in said bacteria.
GB7913033A 1978-04-14 1979-04-12 Vectors derived from lambda bacteriophage Expired GB2018778B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7811109A FR2422685A1 (en) 1978-04-14 1978-04-14 VECTORS, DERIVED FROM LAMBDA BACTERIOPHAGUS, ALLOWING THE INSERTION AND EXPRESSION OF A GENE, PROCARYOTE OR EUKARYOTE, UNDER THE CONTROL OF THE PROMOTER AND THE OPERATOR OF THE LACTOSE OPERON OF ESCHERICHIA COLI BACTERIA

Publications (2)

Publication Number Publication Date
GB2018778A true GB2018778A (en) 1979-10-24
GB2018778B GB2018778B (en) 1982-08-25

Family

ID=9207171

Family Applications (1)

Application Number Title Priority Date Filing Date
GB7913033A Expired GB2018778B (en) 1978-04-14 1979-04-12 Vectors derived from lambda bacteriophage

Country Status (7)

Country Link
JP (1) JPS54141790A (en)
BE (1) BE875596A (en)
DE (1) DE2915081A1 (en)
FR (1) FR2422685A1 (en)
GB (1) GB2018778B (en)
IT (1) IT1119726B (en)
NL (1) NL7902910A (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0095934A2 (en) * 1982-06-02 1983-12-07 Noda Institute For Scientific Research Novel bacteriophage and method for breeding thereof
US4460689A (en) * 1982-04-15 1984-07-17 Merck & Co., Inc. DNA Cloning vector TG1, derivatives, and processes of making
US4508826A (en) * 1982-04-15 1985-04-02 Merck & Co., Inc. Bacteriophage DNA cloning vector TG1 and microorganisms containing TG1
US4874702A (en) * 1980-09-08 1989-10-17 Biogen, Inc. Vectors and methods for making such vectors and for expressive cloned genes
US6268122B1 (en) 1978-12-22 2001-07-31 Biogen, Inc. Recombinant DNA molecules and their method of production

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU527165B2 (en) * 1977-02-08 1983-02-17 Genentech Inc. Method and means for microbial polypeptide expression; synthetic DNA and process therefor
FR2480779B2 (en) * 1979-08-30 1986-07-18 Anvar VECTOR CONTAINING A NUCLEOTIDE SEQUENCE OF THE SURFACE ANTIGEN OF HEPATITIS B VIRUS AND METHOD FOR MANUFACTURING AN IMMUNOGENIC MOLECULE USING THE SAME
AU8746582A (en) * 1981-09-02 1983-03-10 Biogen N.V. Hepatitis b virus e type antigen
DE3484078D1 (en) * 1983-06-27 1991-03-14 Genentech Inc TRANSFERABLE INDUCIBLE CONTROL SYSTEMS, THESE EXPRESSION VECTORS, WITH THESE TRANSFORMED MICROORGANISMS AND THEIR USE IN EXPRESSING EXOGENIC PROTEIN.
CA1278540C (en) * 1983-07-22 1991-01-02 Eli Lilly And Company Modified antibiotic resistance gene

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4190495A (en) * 1976-09-27 1980-02-26 Research Corporation Modified microorganisms and method of preparing and using same

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6268122B1 (en) 1978-12-22 2001-07-31 Biogen, Inc. Recombinant DNA molecules and their method of production
US6270955B1 (en) 1978-12-22 2001-08-07 Biogen, Inc. Pharmaceutical compositions and methods for producing antibodies to hepatitis b virus and kits and methods for detecting antibodies to hepatitis b virus
US6297355B1 (en) 1978-12-22 2001-10-02 Biogen, Inc. Polypeptides displaying HBV antigenicity or hbv antigen specificity
US4874702A (en) * 1980-09-08 1989-10-17 Biogen, Inc. Vectors and methods for making such vectors and for expressive cloned genes
US4460689A (en) * 1982-04-15 1984-07-17 Merck & Co., Inc. DNA Cloning vector TG1, derivatives, and processes of making
US4508826A (en) * 1982-04-15 1985-04-02 Merck & Co., Inc. Bacteriophage DNA cloning vector TG1 and microorganisms containing TG1
EP0095934A2 (en) * 1982-06-02 1983-12-07 Noda Institute For Scientific Research Novel bacteriophage and method for breeding thereof
EP0095934A3 (en) * 1982-06-02 1985-08-14 Noda Institute For Scientific Research Novel bacteriophage and method for breeding thereof

Also Published As

Publication number Publication date
IT1119726B (en) 1986-03-10
BE875596A (en) 1979-10-15
FR2422685A1 (en) 1979-11-09
GB2018778B (en) 1982-08-25
FR2422685B1 (en) 1980-10-31
IT7921839A0 (en) 1979-04-13
DE2915081A1 (en) 1979-10-25
JPS54141790A (en) 1979-11-05
NL7902910A (en) 1979-10-16

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Legal Events

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PCNP Patent ceased through non-payment of renewal fee