GB1503828A - Method of enumerating bacteria - Google Patents
Method of enumerating bacteriaInfo
- Publication number
- GB1503828A GB1503828A GB2580276A GB2580276A GB1503828A GB 1503828 A GB1503828 A GB 1503828A GB 2580276 A GB2580276 A GB 2580276A GB 2580276 A GB2580276 A GB 2580276A GB 1503828 A GB1503828 A GB 1503828A
- Authority
- GB
- United Kingdom
- Prior art keywords
- organisms
- micro
- phosphate
- liquid
- derivatives
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 241000894006 Bacteria Species 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 244000005700 microbiome Species 0.000 abstract 7
- 239000007788 liquid Substances 0.000 abstract 5
- 150000003013 phosphoric acid derivatives Chemical class 0.000 abstract 4
- BFMYDTVEBKDAKJ-UHFFFAOYSA-L disodium;(2',7'-dibromo-3',6'-dioxido-3-oxospiro[2-benzofuran-1,9'-xanthene]-4'-yl)mercury;hydrate Chemical compound O.[Na+].[Na+].O1C(=O)C2=CC=CC=C2C21C1=CC(Br)=C([O-])C([Hg])=C1OC1=C2C=C(Br)C([O-])=C1 BFMYDTVEBKDAKJ-UHFFFAOYSA-L 0.000 abstract 3
- RAHZWNYVWXNFOC-UHFFFAOYSA-N Sulphur dioxide Chemical compound O=S=O RAHZWNYVWXNFOC-UHFFFAOYSA-N 0.000 abstract 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 abstract 2
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 abstract 2
- 239000000758 substrate Substances 0.000 abstract 2
- 238000005282 brightening Methods 0.000 abstract 1
- 238000007385 chemical modification Methods 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 230000032050 esterification Effects 0.000 abstract 1
- 238000005886 esterification reaction Methods 0.000 abstract 1
- 239000000834 fixative Substances 0.000 abstract 1
- 230000007062 hydrolysis Effects 0.000 abstract 1
- 238000006460 hydrolysis reaction Methods 0.000 abstract 1
- 230000011987 methylation Effects 0.000 abstract 1
- 238000007069 methylation reaction Methods 0.000 abstract 1
- 230000003287 optical effect Effects 0.000 abstract 1
- 230000003647 oxidation Effects 0.000 abstract 1
- 238000007254 oxidation reaction Methods 0.000 abstract 1
- 238000010186 staining Methods 0.000 abstract 1
- 235000010269 sulphur dioxide Nutrition 0.000 abstract 1
- 239000004291 sulphur dioxide Substances 0.000 abstract 1
- 239000000725 suspension Substances 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2304/00—Chemical means of detecting microorganisms
- C12Q2304/10—DNA staining
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Toxicology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
1503828 Enumerating bacteria STRATHCLYDE UNIVERSITY OF 22 June 1976 25802/76 Heading C6F A method of staining micro-organisms to facilitate counting comprises the steps of providing a suspension of micro-organisms in a liquid medium, reacting the micro-organisms in the suspended state with phosphate ions whereby phosphate ions react at active sites on the micro-organisms to produce phosphate derivatives of the micro-organisms having at said sites polydentate phosphate groups, and treating the phosphate derivatives with a phosphate-reactive fluorochrome dye (specified) which thereby combines chemically with the microorganisms via the intermediate phosphate groups. Preferably the phosphate derivatives are treated with the fluorochrome dye in the suspended state in liquid medium to produce a liquid specimen for microscopic examination in the liquid state. Alternatively, the phosphate derivatives are applied to a carrier substrate and fixed thereto using a liquid fixative, and the fixed sample is brought into contact with the fluorochrome dye to be stained, the stained sample being dried to provide on the substrate a dry specimen for microscopic examination in the dry state. The micro-organisms may be subjected to further chemical modifications e.g. methylation, esterification, hydrolysis, oxidation or reaction with sulphur dioxide, whereby to increase the number of active sites available. They may also be treated with an optical brightening agent. Many detailed Examples are provided.
Priority Applications (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB2580276A GB1503828A (en) | 1976-06-22 | 1976-06-22 | Method of enumerating bacteria |
| CA280,989A CA1089339A (en) | 1976-06-22 | 1977-06-20 | Method of staining micro-organisms |
| FR7718920A FR2355912A1 (en) | 1976-06-22 | 1977-06-21 | MICROORGANISMS STAINING PROCESS |
| DK276677A DK276677A (en) | 1976-06-22 | 1977-06-22 | PROCEDURES FOR COLORING MICRO-ORGANISMS |
| DE19772728077 DE2728077A1 (en) | 1976-06-22 | 1977-06-22 | METHOD OF COLORING MICRO-ORGANISMS |
| JP7339577A JPS5320479A (en) | 1976-06-22 | 1977-06-22 | Dyeing and counting of microorganism |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB2580276A GB1503828A (en) | 1976-06-22 | 1976-06-22 | Method of enumerating bacteria |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1503828A true GB1503828A (en) | 1978-03-15 |
Family
ID=10233555
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB2580276A Expired GB1503828A (en) | 1976-06-22 | 1976-06-22 | Method of enumerating bacteria |
Country Status (6)
| Country | Link |
|---|---|
| JP (1) | JPS5320479A (en) |
| CA (1) | CA1089339A (en) |
| DE (1) | DE2728077A1 (en) |
| DK (1) | DK276677A (en) |
| FR (1) | FR2355912A1 (en) |
| GB (1) | GB1503828A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1985005691A1 (en) * | 1984-05-25 | 1985-12-19 | University Of Strathclyde | Apparatus for microscopic examination |
| WO1999060373A1 (en) * | 1998-05-20 | 1999-11-25 | Nanomont Gesellschaft Für Nanotechnologie Mbh | Method and device for fixing micro- and/or nano-objects |
| WO2001036661A2 (en) * | 1999-10-25 | 2001-05-25 | Genprime, Inc. | Method and apparatus for prokaryotic and eukaryotic cell quantitation |
| US6673568B1 (en) | 1999-10-25 | 2004-01-06 | Genprime, Inc. | Method and apparatus for prokaryotic and eukaryotic cell quantitation |
| US6787302B2 (en) | 1999-10-25 | 2004-09-07 | Genprime, Inc. | Method and apparatus for prokaryotic and eukaryotic cell quantitation |
| CN112575054A (en) * | 2019-12-16 | 2021-03-30 | 中国计量科学研究院 | Rapid synchronous multiple detection method and kit for total number of listeria monocytogenes and bacteria |
| CN113430107A (en) * | 2021-06-25 | 2021-09-24 | 常州市金坛第一人民医院 | Modular pretreatment system for microbial detection |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1112987A (en) * | 1978-09-25 | 1981-11-24 | Joseph L. Melnick | Staining and analysis of bacteria |
| JPH0782009B2 (en) * | 1988-03-24 | 1995-09-06 | 工業技術院長 | Cell fusion method and device |
| DE602004010323T2 (en) | 2003-03-27 | 2008-03-13 | Nissan Chemical Industries, Ltd. | Diantimony pentoxide sol and method of preparation |
| WO2006033283A1 (en) | 2004-09-21 | 2006-03-30 | Nissan Chemical Industries, Ltd. | Process for producing antimony pentaoxide |
| CN103091145B (en) * | 2013-01-30 | 2015-04-29 | 中国人民解放军军事医学科学院生物工程研究所 | Method for staining microorganisms |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3678151A (en) * | 1969-07-25 | 1972-07-18 | Gugol Clini Tex Inc | Biological staining method |
| DE2429380A1 (en) * | 1973-06-22 | 1975-01-23 | Univ Strathclyde Glasgow | METHOD OF COUNTING AND COLORING MICRO-ORGANISMS |
-
1976
- 1976-06-22 GB GB2580276A patent/GB1503828A/en not_active Expired
-
1977
- 1977-06-20 CA CA280,989A patent/CA1089339A/en not_active Expired
- 1977-06-21 FR FR7718920A patent/FR2355912A1/en active Granted
- 1977-06-22 DK DK276677A patent/DK276677A/en not_active Application Discontinuation
- 1977-06-22 JP JP7339577A patent/JPS5320479A/en active Pending
- 1977-06-22 DE DE19772728077 patent/DE2728077A1/en not_active Withdrawn
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1985005691A1 (en) * | 1984-05-25 | 1985-12-19 | University Of Strathclyde | Apparatus for microscopic examination |
| WO1999060373A1 (en) * | 1998-05-20 | 1999-11-25 | Nanomont Gesellschaft Für Nanotechnologie Mbh | Method and device for fixing micro- and/or nano-objects |
| WO2001036661A2 (en) * | 1999-10-25 | 2001-05-25 | Genprime, Inc. | Method and apparatus for prokaryotic and eukaryotic cell quantitation |
| WO2001036661A3 (en) * | 1999-10-25 | 2002-07-11 | Genprime Inc | Method and apparatus for prokaryotic and eukaryotic cell quantitation |
| US6673568B1 (en) | 1999-10-25 | 2004-01-06 | Genprime, Inc. | Method and apparatus for prokaryotic and eukaryotic cell quantitation |
| US6787302B2 (en) | 1999-10-25 | 2004-09-07 | Genprime, Inc. | Method and apparatus for prokaryotic and eukaryotic cell quantitation |
| CN112575054A (en) * | 2019-12-16 | 2021-03-30 | 中国计量科学研究院 | Rapid synchronous multiple detection method and kit for total number of listeria monocytogenes and bacteria |
| CN113430107A (en) * | 2021-06-25 | 2021-09-24 | 常州市金坛第一人民医院 | Modular pretreatment system for microbial detection |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5320479A (en) | 1978-02-24 |
| FR2355912A1 (en) | 1978-01-20 |
| CA1089339A (en) | 1980-11-11 |
| FR2355912B1 (en) | 1983-12-16 |
| DE2728077A1 (en) | 1978-01-05 |
| DK276677A (en) | 1977-12-23 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PS | Patent sealed | ||
| 732 | Registration of transactions, instruments or events in the register (sect. 32/1977) | ||
| 732 | Registration of transactions, instruments or events in the register (sect. 32/1977) | ||
| PCNP | Patent ceased through non-payment of renewal fee |
Effective date: 19930622 |