FR2812544A1 - Treatment of cutaneous aging symptoms, such as wrinkles, comprises topical or oral administration of Salvia genus plant extracts with collagenase expression inhibiting activity - Google Patents

Abstract

Use of extract(s) (A) of Salvia genus plants is claimed in (or for the preparation of) compositions for (i) treating symptoms of cutaneous aging, (ii) inhibiting the expression of extracellular matrix proteases (specifically metalloproteinases, especially metalloprotease-1 (MMP-1; i.e. collagenase); and/or (iii) combating internal degradation of the skin (especially degradation of collagen) caused by exposure to ultraviolet radiation. Independent claims are included for corresponding cosmetic treatment methods using (A).

Description

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The invention relates to the use in a composition or for the preparation of a composition of at least one extract of at least one plant of the genus Salvia, the extract or the composition being intended for treating the cutaneous signs of aging. In particular, the extract or the composition is intended to inhibit the degradation of the skin and / or mucous membranes by the inhibition of collagenases. The subject of the invention is also a process for cosmetic treatment of the skin and / or mucous membranes.

The human skin consists of two compartments namely a superficial compartment, the epidermis, and a deep compartment, the dermis. The natural human epidermis is composed mainly of three types of cells, which are the keratinocytes, a very large majority, melanocytes and Langerhans cells. Each of these cell types contributes by its own functions to the essential role played in the body by the skin.

The dermis provides the epidermis with a solid support. It is also its nurturing element. It consists mainly of fibroblasts and an extracellular matrix composed mainly of collagen, elastin and a substance called the fundamental substance, components synthesized by the fibroblast. There are also leucocytes, mast cells or tissue macrophages. It is also crossed by blood vessels and nerve fibers. In normal skin, ie non-pathological or scarring, the fibroblast is in the quiescent state, ie non-proliferative, not very active from a metabolic and non-mobile point of view.

It is the collagen fibers that ensure the solidity of the dermis. The collagen fibers consist of fibrils sealed to each other, thus forming more than ten different types of structures. The strength of the dermis is largely due to the entanglement of collagen fibers packed against each other in all directions. Collagen fibers contribute to the elasticity and tone of the skin and / or mucous membranes.

The collagen fibers are constantly renewed but this renewal decreases with age which causes a thinning of the dermis. This thinning of the dermis is also due to pathological causes such as the hypersecretion of corticosteroid hormones, certain pathologies or vitamin deficiencies (case of vitamin C in scurvy). It is also accepted that extrinsic factors such as ultraviolet rays,

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 tobacco or certain treatments (Glucocorticoids, vitamin D and derivatives for example) also have an effect on the skin and on its collagen level. However, various factors cause collagen degradation, with all the consequences that can be considered on the structure and / or the firmness of the skin and / or mucous membranes.

Although highly resistant, collagen fibers are sensitive to certain enzymes called collagenases. A breakdown of collagen fibers causes the appearance of soft, wrinkled skin that the human being, preferring the appearance of a smooth and tense skin, has always sought to fight. Collagenases are part of a family of enzymes called metal loproteinases (MMPs) that are themselves members of a family of proteolytic enzymes (endoproteases) that have a coordinated 3-cysteine zinc atom and a methionine in their active site and which degrade the macromolecular components of the extracellular matrix and basal slides at neutral pH (collagen, elastin, etc. ...). Very widespread in the living world, these enzymes are present, but weakly expressed, in normal physiological situations such as organ growth and tissue renewal.

Their overexpression in humans and their activation are however linked to many processes, sometimes pathological, which involve the destruction and remodeling of the matrix. This results in either uncontrolled resorption of the extracellular matrix, or conversely the installation of a state of fibrosis.

The metalloproteinase family consists of several well-defined groups based on their similarities in structure and substrate specificity (see Woessner, J. F., Faseb Journal, vol 5, 1991, 2145). Among these groups, there may be mentioned collagenases intended to degrade fibrillar collagens (MMP-1 or interstitial collagenase, MMP-8 or neutrophil collagenase, MMP-13 or collagenase 3), gelatinases which degrade type IV collagen or any other type of collagenase. denatured collagen form (MMP-2 or gelatinase A (72 kDa), MMP-9 or gelatinase B (92 kDa)), stromelysins (MMP-3) whose broad spectrum of activity is directed to the proteins of the matrix extracellular such as glycoproteins (fibronectin, laminin), proteoglycans, etc., or membrane loproteinase metal.

Prolonged exposure to ultraviolet radiation, particularly ultraviolet light type A and / or B, has the effect of stimulating

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 the expression of collagenases, particularly MMP-1. This is one of the components of photo-induced skin aging.

In addition, during menopause, the main changes in the dermis are a decrease in collagen level and dermal thickness. This leads to a thinning of the skin and / or mucous membranes in the menopausal woman. The woman feels a sensation of "dry skin" or skin that pulls and there is an accentuation of fine lines and wrinkles surface. The skin has a rough appearance on palpation. Finally the skin has a reduced flexibility.

It is then understood from reading the foregoing the importance of collagen in the structure of tissues, particularly of the skin and / or mucous membranes, and the importance of combating its degradation to thereby combat aging. chronobiological or photoinduced and its consequences, thinning of the dermis and / or degradation of collagen fibers which cause the appearance of soft and wrinkled skin.

One of the aims of the present invention is therefore to be able to have a new product that has an inhibitory effect on collagenases and if possible no significant side effects.

Surprisingly and unexpectedly, the Applicant has now discovered that an extract of at least one plant of the genus Salvia has an inhibitory activity of the activity of collagenases.

The plants of the genus Salvia belong to the Labie family.

They are known and used, in addition to their taste properties, for their antiseptic, antispasmodic, antiperspirant, carminative, choleretic, emmenagogues, hypocalcemic, stimulating, stomachic or even vulnerary properties.

To the knowledge of the applicant, the inhibitory activity of the activity of collagenases of at least one extract of at least one plant of the genus Salvia has never been described.

Thus, the subject of the invention is primarily the use in a composition or for the preparation of a composition of at least one extract of at least one plant from

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 Salvia genus, the extract or the composition being intended to treat the cutaneous signs of aging.

The use of the extract or the composition may be considered in a curative and / or preventive manner to the cutaneous signs of aging.

By cutaneous signs of aging is meant any changes in the external appearance of the skin due to aging whether chronobiological and / or photoinduced, such as, for example, lines and wrinkles, wilted skin, mole skin, skin thinned, lack of elasticity and / or tone of the skin, but also any internal changes in the skin that do not systematically result in a modified external appearance, such as any internal damage to the skin, particularly collagen, following exposure to ultraviolet radiation.

The subject of the invention is the use in a composition or for the preparation of a composition of at least one extract of at least one plant of the genus Salvia, the extract or the composition being intended to inhibit the expression proteases of the extracellular matrix, particularly metal loproteinases and even more particularly metallo-proteinase type 1.

The third subject of the invention is the use in a composition or for the preparation of a composition of at least one extract of at least one plant of the genus Salvia, the extract or the composition being intended for treating cutaneous lesions. related to menopause.

The fourth subject of the invention is the use in a composition or for the preparation of a composition of at least one extract of at least one plant of the genus Salvia, the extract or the composition being intended for combating wrinkles. and fine lines.

The invention has the fifth object the use in a composition or for the preparation of a composition of at least one extract of at least one plant of the genus Salvia, the extract or the composition being intended to fight against the skin withered.

The sixth subject of the invention is the use in a composition or for the preparation of a composition of at least one extract of at least one plant from

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 Salvia genus, the extract or composition being intended to combat soft skin.

The seventh object of the invention is the use in a composition or for the preparation of a composition of at least one extract of at least one plant of the genus Salvia, the extract or the composition being intended to fight against the skin thinned.

The invention has for its eighth object the use in a composition or for the preparation of a composition of at least one extract of at least one plant of the genus Salvia, the extract or the composition being intended to combat the lack of elasticity and / or tone of the skin.

By extract of at least one plant of the genus Salvia is meant both a crude mixture of parts of the plant roughly reduced in pieces and the extraction solvent as prepared preparations solubilized active principles during extraction.

By active ingredient is meant any molecule or composition capable of modifying or modulating the operation of at least one biological system.

The extract of at least one plant of the genus Salvia used according to the invention can be obtained from plant material derived from whole plant or part of plant such as leaves, stems, flowers, petals, roots or still dedifferentiated cells.

By dedifferentiated plant cells is meant any plant cell having none of the characters of a particular specialization and capable of living on its own and not dependent on other cells.

Preferentially according to the invention, the whole plant is used, particularly the stem and / or leaves, very particularly leaves. The extract of at least one plant of the genus Salvia can be any extract prepared from any plant material derived from at least one plant of the genus Salvia grown in vivo or derived from in vitro culture.

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 By in vivo culture is meant any culture of conventional type, ie in soil in the open air or in the greenhouse, or above ground.

By in vitro culture is meant all the techniques known to those skilled in the art which artificially allows the production of a plant or part of a plant. The selection pressure imposed by the physicochemical conditions during the growth of the plant cells in vitro makes it possible to obtain a standardized plant material that is available throughout the year, unlike plants grown in vivo.

Preferably, according to the invention, a plant of the genus Salvia derived from culture in vivo is used.

The genus Salvia belongs to the family Labiés and consists of 19 species among which we can cite as an example Salvia officinalis (great sage), Salvia Sclarea (clary sage) or Salvia pratensis (sage of the meadows); The extract of the invention is preferably prepared from plant material derived from the species Salvia ofcinalis.

Any extraction method known to those skilled in the art can be used to prepare the extract according to the invention.

In particular, mention may be made of alcoholic extracts, in particular ethanolic extracts. Preferably according to the invention, the extract is an aqueous extract.

By aqueous extract is meant an extract whose solvent is totally or partly water. We can thus mention the water itself, hydroalcoholic solvents in any proportion or solvents consisting of water and a compound such as propylene glycol in any proportion.

It is also possible to use an extract prepared by the method described in the French patent application No. 95-02379 filed by the applicant.

Thus, in a first step the plant material is milled in a cold aqueous solution and in a second step the suspended particles are removed from the aqueous solution from the first stage. This aqueous solution corresponds to the extract.

 Optionally, in a third step, the aqueous solution resulting from the second step is sterilized.

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 This extract can then be lyophilized.

The first step may advantageously be replaced by a simple freezing operation of the plant tissues (for example at -20 ° C. or else at -18 ° C. in liquid nitrogen), followed by an aqueous extraction taking up the second and third steps below. described above.

The cold treatment makes it possible to freeze the enzymatic activities, the sterilizing filtration avoids the degradation of the active ingredients by the microorganisms of the environment. Finally, the water vehicle is compatible with ex vivo receptors and facilitates cosmetic or pharmaceutical formulations.

It is known that plant extracts contain oxidases responsible, inter alia, for the oxidation of said extracts. However, such an oxidation leads to a dark brown color of the extracts and to a pungent odor rendering them incompatible with their use in cosmetics. In this connection, a laccase having a molecular weight greater than 100,000 daltons is particularly known.

Thus, advantageously, the extract obtained can be fractionated by any known fractionation method for eliminating oxidases and in particular polyphenoloxidase. For example, the extract of the invention can be filtered on a dialysis membrane in order to eliminate molecules with a molecular weight greater than 100,000 daltons. It is also possible to subject the extract to fractionation by selective precipitation.

Other methods make it possible to protect oneself from oxidation phenomena. In particular, the extract can also be stabilized. Any known stabilization method can be used according to the invention. For example, the extract of the invention can be stabilized by sparging nitrogen to remove dissolved oxygen or by addition of cysteine and / or sulfur derivatives to a final concentration of between 0.5 g / l and 10 g / l and preferably between 1 g / l and 2.5 g / l.

Obviously the extract according to the invention can be fractionated and stabilized.

By way of example, according to the invention, an extract of Salvia officinalis prepared by the company Flachsmann (Germany), marketed under the reference 085282, consisting of an aqueous extract of Salvia officinalis leaves is used.

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 The extract can constitute by itself the active ingredient of the compositions of the invention. The amount of extract that can be used according to the invention is, of course, a function of the desired effect and can therefore vary to a large extent.

To give an order of magnitude, the extract can be used in an amount representing 0.00001% to 20% of the total weight of the composition and preferably in an amount representing from 0.0001% to 10% of the total weight of the composition.

The composition of the invention may be in any conceivable dosage form, suitable both for topical application to the skin and / or mucous membranes and / or the hair, as well as to oral administration.

Preferably, the composition of the invention is intended for administration by the oral route.

The composition of the invention may be a cosmetic or dermatological composition. Preferably according to the invention, the composition is a cosmetic composition. The composition is a cosmetic composition because it is intended to improve the general cutaneous appearance of the individual who makes use of it. Very preferably, the composition of the invention is a cosmetic composition intended for administration by the oral route.

For oral administration, the composition of the invention may be in any suitable form, particularly in the form of an oral solution such as, for example, a syrup, a tablet, a capsule, a capsule or a nutritional food or a nutritional supplement.

The composition further comprises at least one suitable excipient suitable for oral administration.

For administration by topical application to the skin, the hair and / or the mucous membranes, the composition according to the invention obviously comprises a cosmetically acceptable support, ie a support compatible with the skin, the mucous membranes, the nails, hair and can stand under any

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 the dosage forms normally used for topical application, especially in the form of an aqueous, hydroalcoholic or oily solution, an oil-in-water or water-in-oil or multiple emulsion, an aqueous or oily gel, a liquid, pasty or solid anhydrous product, an oil dispersion in an aqueous phase using spherules, these spherules may be polymeric nanoparticles such as nanospheres and nanocapsules or better lipid vesicles of ionic type and / or nonionic.

This composition may be more or less fluid and have the appearance of a white or colored cream, an ointment, a milk, a lotion, a serum, a paste, a mousse . It can optionally be applied to the skin in the form of an aerosol. It can also be in solid form, and for example in the form of a stick. It can be used as a care product, as a cleaning product, as a makeup product or as a simple deodorant product.

In known manner, the composition of the invention may contain the usual adjuvants in the cosmetic and dermatological fields, such as hydrophilic or lipophilic gelling agents, hydrophilic or lipophilic active agents, preservatives, antioxidants, solvents, perfumes, fillers , filters, pigments, chelating agents, odor absorbers and dyestuffs. The amounts of these various adjuvants are those conventionally used in the fields under consideration, and for example from 0.01 to 20% of the total weight of the composition. These adjuvants, depending on their nature, can be introduced into the fatty phase, into the aqueous phase, into the lipid vesicles and / or into the nanoparticles.

When the composition of the invention is an emulsion, the proportion of the fatty phase can range from 5% to 80% by weight, and preferably from 5% to 50% of the total weight of the composition. The oils, emulsifiers and coemulsifiers used in the composition in emulsion form are chosen from those conventionally used in the field under consideration. The emulsifier and the coemulsifier are present in the composition in a proportion ranging from 0.3% to 30% by weight, and preferably from 0.5% to 20% of the total weight of the composition.

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 As oils that can be used in the invention, mention may be made of mineral oils, oils of vegetable origin (apricot oil, sunflower oil), oils of animal origin, synthetic oils, silicone oils and oils. fluorinated (perfluoropolyethers). It is also possible to use fatty alcohols (cetyl alcohol), fatty acids and waxes (beeswax) as fatty substances.

As emulsifiers and coemulsifiers that can be used in the invention, mention may be made, for example, of fatty acid and polyethylene glycol esters such as PEG-40 stearate, PEG-100 stearate, fatty acid and polyol esters. such as glyceryl stearate and sorbitan tristearate.

As hydrophilic gelling agents, mention may in particular be made of carboxyvinyl polymers (carbomer), acrylic copolymers such as copolymers of acrylates / alkylacrylates, polyacrylamides, polysaccharides, natural gums and clays, and, as lipophilic gelling agents, it is possible to mention mention modified clays such as bentones, metal salts of fatty acids, hydrophobic silica and polyethylenes.

The present invention further relates to a cosmetic treatment method for treating cutaneous signs of aging, ie any changes in the external appearance of the skin due to aging whether chronobiological and / or photoinduced, such as wrinkles and fine lines, wilted skin, mole skin, thinned skin, lack of elasticity and / or tone of the skin, but also any internal changes in the skin that do not always translate into a modified external appearance, such as, for example, any internal degradation of the skin, particularly collagen, following exposure to ultraviolet radiation, characterized by the fact that it is applied to the skin, to the hair, and / or to the mucous membranes, or that a cosmetic composition comprising at least one or more extracts of at least one plant of the genus Salvia is ingested.

The cosmetic treatment method of the invention may be used in particular by applying the cosmetic compositions as defined above, according to the usual technique of use of these compositions. For example: application of creams, gels, serums, lotions, milks, shampoos or sunscreens, on the skin, or on the hair or application of toothpaste on the gums and preferably by administration oral solution of an oral solution, a tablet, a

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 capsule or a capsule or a nutritional food or nutritional supplement.

The following examples and compositions illustrate the invention without limiting it in any way. In the compositions, the proportions indicated are percentages by weight.

Example 1 Effect of an extract of at least one plant of the genus Salvia on the expression of collagenases.

The effect of an extract of at least one plant of the genus Salvia on the production of interstitial collagenase was evaluated in a culture model of A2058 cells (from human melanomas: Templeton NS and a1.1990; Cancer Res. , 50: 5431-5431).

The extract tested is that sold by Flachsmann (Germany), sold under the reference 085282, consisting of an aqueous extract of Salvia officinalis leaves.

The cells are incubated in a phenol-free MEM medium containing amino acids at a concentration of 2 mM, sodium pyruvate at a concentration of 1 mM and calf serum charcoal at 10%. They are then cultured at a density of 20000 cells per well in the 24 wells of multiwell plate.

Twenty four hours after culturing, the cells are brought into contact with an extract of at least one plant of the genus Salvia. The production of interstitial collagenase is evaluated 96 hours later in the culture medium. This is carried out using an Elisa kit (Biotrack human MMP1, Amersham) The extract of at least one plant of the genus Salvia is tested at 0.005%, 0.05% and 0.5%.

The results expressed as a percentage represent the decrease in the production of interstitial collagenase by control, ie by contribution to a culture carried out under the same conditions in the absence of resveratrol.

<tb> Salvia <SEP> 0.005% <SEP> 0.05% <SEP> 0.5%
<tb> inhibition <SEP> 11% <SEP> 75% <SEP> 100%

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 The results show that the extract of at least one plant of the genus Salvia decreases the production of interstitial collagenase by A 2058 cells in a dose-dependent manner.

Example 2: Examples of formulations illustrating the invention and particularly the compositions according to the invention. These compositions were obtained according to the techniques conventionally used in the cosmetics industry.

<tb> Composition <SEP> 1 <SEP> - <SEP> Soft SEP Capsules
<tb> Excipients
<tb> Oil <SEP> of <SEP> Soybeans <SEP> 40 <SEP> mg
<tb> Oil <SEP> of <SEP> Sprout <SEP> of <SEP> Wheat <SEP> 85 <SEP> mg
<tb> Lecithins <SEP> of <SEP> Soybeans <SEP> 25 <SEP> mg
<tb> Vitamin
<tb> Natural Tocopherols <SEP><SEP> 3 <SEP> mg
<tb> Components
<tb> Extract <SEP> of <SEP> Sage * <SEP> Dehydrated <SEP> 5 <SEP> mg
<tb> Composition <SEP> 2 <SEP>: <SEP> Shampoo
<tb> Extract <SEP> of <SEP> Sage * <SEP> 2.00
<tb> Hydroxypropylcellulose <SEP> (Klucel <SEP> H <SEP> sold <SEP> by <SEP> the <SEP> company <SEP> Hercules) <SEP> 1.00
<tb> Perfume <SEP> 0,50
<tb> Conservative <SEP> 0.30
<tb> Water <SEP> qsp <SEP> 100,00
<tb> Composition <SEP> 3 <SEP>: <SEP> Cream <SEP> of <SEP> care <SEP> of <SEP> face <SEP> (emulsion <SEP> oil <SEP> in <SEP> water)
<tb> Extract <SEP> of <SEP> Sage * <SEP> 1.00
<tb> Stearate <SEP> of <SEP> glycerol <SEP> 2.00
<tb> Polysorbate <SEP> 60 <SEP> (Tween <SEP> 60 <SEP> sold <SEP> by <SEP> the <SEP> Company <SEP> HERE) <SEP> 1.00
<tb> Stearic acid <SEP><SEP> 1.40
<tb> Triethanolamine <SEP> 0.70
<tb> Carbomer <SEP> 0.40
<tb> Fraction <SEP> liquid <SEP> of <SEP> butter <SEP> of <SEP> shea butter <SEP> 12,00
<tb> Perhydrosqualene <SEP> 12.00
<tb> Antioxidant <SEP> 0.05
<tb> Perfume <SEP> 0,50

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<tb> Conservative <SEP> 0.30
<tb> Water <SEP> qsp <SEP> 100,00
<tb> Composition <SEP> 4 <SEP>: <SEP> Gel <SEP> for <SEP> the <SEP> skin
<tb> Extract <SEP> of <SEP> Sage * <SEP> 0.10
<tb> Acid <SEP> all <SEP> trans <SEP> retinoic <SEP> 0.05
<tb> Hydroxypropylcellulose <SEP> (Klucel <SEP> H sold <SEP> by <SEP> the <SEP> company <SEP> Hercules) <SEP> 1.00
<tb> Antioxidant <SEP> 0.05
<tb> Isopropanol <SEP> 40.00
<tb> Conservative <SEP> 0.30
<tb> Water <SEP> qsp <SEP> 100,00
<tb> Composition <SEP> 5 <SEP>: <SEP> Gel <SEP> for <SEP> the <SEP> care <SEP> of the <SEP> face
<tb> Extract <SEP> of <SEP> Sage * <SEP> 0.30
<tb> Hydroxypropylcellulose <SEP> (Klucel <SEP> H <SEP> sold <SEP> by <SEP> the <SEP> company <SEP> Hercules) <SEP> 1.00
<tb> Antioxidant <SEP> 0.05
<tb> Isopropanol <SEP> 40.00
<tb> Conservative <SEP> 0.30
<tb> Water <SEP> qsp <SEP> 100,00
<tb> Composition <SEP> 6 <SEP>: <SEP> Gel
<tb> Extract <SEP> from <SEP> Sage * <SEP> 2.50
<tb> Hydroxypropylcellulose <SEP> (Klucel <SEP> H <SEP> sold <SEP> by <SEP> the <SEP> company <SEP> Hercules) <SEP> 1.00
<tb> Antioxidant <SEP> 0.05
<tb> Hydrochloride <SEP> of <SEP> lidocaine <SEP> 2.00
<tb> Isopropanol <SEP> 40.00
<tb> Conservative <SEP> 0.30
<tb> Water <SEP> qsp <SEP> 100,00
<tb> Composition <SEP> 7 <SEP>: <SEP> Cream <SEP> of <SEP> care <SEP> of <SEP> erythema <SEP> solar <SEP> (oil-in-oil emulsion <SEP> water)
<tb> Extract <SEP> of <SEP> Sage * <SEP> 0.05
<tb> Stearate <SEP> of <SEP> glycerol <SEP> 2.00
<tb> Polysorbate <SEP> 60 <SEP> (Tween <SEP> 60 <SEP> sold <SEP> by <SEP> the <SEP> Company <SEP> HERE) <SEP> 1.00
<tb> Stearic acid <SEP><SEP> 1.40
<tb><SEP> Glycyrrhetinic acid <SEP> 2.00
<tb> Triethanolamine <SEP> 0.70
<tb> Carbomer <SEP> 0.40
<tb> Fraction <SEP> liquid <SEP> of <SEP> butter <SEP> of <SEP> shea butter <SEP> 12,00

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<tb> Oil <SEP> of <SEP> Sunflower <SEP> 10.00
<tb> Antioxidant <SEP> 0.05
<tb> Perfume <SEP> 0,50
<tb> Conservative <SEP> 0.30
<tb> Water <SEP> qsp <SEP> 100,00
<tb> Composition <SEP> 8 <SEP>: <SEP> Cream <SEP> of <SEP> skincare <SEP> anti-wrinkle <SEP> for <SEP><SEP> face <SEP> (emulsion <SEP> oil / water)
<tb> Extract <SEP> of <SEP> Sage * <SEP> 0.50
<tb> Stearate <SEP> of <SEP> glycerol <SEP> 2.00
<tb> Polysorbate <SEP> 60 <SEP> (Tween <SEP> 60 <SEP> sold <SEP> by <SEP> the <SEP> Company <SEP> HERE) <SEP> 1.00
<tb> Stearic acid <SEP><SEP> 1.40
<tb><SEP> n-octanoyl-5-salicylic acid <SEP> 0.50
<tb> Triethanolamine <SEP> 0.70
<tb> Carbomer <SEP> 0.40
<tb> Fraction <SEP> liquid <SEP> of <SEP> butter <SEP> of <SEP> shea butter <SEP> 12,00
<tb> Perhydrosqualene <SEP> 12.00
<tb> Antioxidant <SEP> 0.05
<tb> Perfume <SEP> 0,50
<tb> Conservative <SEP> 0.30
<tb> Water <SEP> qsp <SEP> 100,00
<tb> Composition <SEP> 9 <SEP>: <SEP> Lotion
<tb> Extract <SEP> of <SEP> Sage * <SEP> 0.15
<tb> Glycolic <SEP> acid <SEP> 50.00
<tb> Hydroxypropylcellulose <SEP> (Klucel <SEP> H <SEP> sold <SEP> by <SEP> the <SEP> company <SEP> Hercules) <SEP> 0.05
<tb> Conservative <SEP> 0.30
<tb> NaOH <SEP> qsp <SEP> pH <SEP> = <SEP> 2,8
<tb> Ethanol <SEP> qsp <SEP> 100,00
<tb> Composition <SEP> 10, <SEP> Lotion <SEP> cleanser <SEP> for <SEP> the <SEP> face
<tb> Extract <SEP> of <SEP> Sage * <SEP> 0.01 <SEP>! o
<tb> Antioxidant <SEP> 0.05
<tb> Isopropanol <SEP> 40.00
<tb> Conservative <SEP> 0.30
<tb> Water <SEP> qsp <SEP> 100,00
<tb> * <SEP>: <SEP> sold <SEP> by <SEP> the <SEP> company <SEP> Flachsmann <SEP> (Germany), <SEP> marketed <SEP> under <SEP> la
<tb> reference <SEP> 085282, <SEP> constituted <SEP> of an <SEP> aqueous <SEP> extract <SEP> of <SEP> leaves <SEP> of <SEP> Salvia <SEP> officinalis.

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Claims (23)

1. Use in a composition or for the preparation of a composition of at least one extract of at least one plant of the genus Salvia, the extract or composition being intended to treat the cutaneous signs of aging. 2. Use in a composition or for the preparation of a composition of at least one extract of at least one plant of the genus Salvia, the extract or the composition being intended to inhibit the expression of proteases of the extracellular matrix. 3. Use according to claim 2, characterized in that the extract or the composition are intended to inhibit the expression of metal loproteinases. 4. Use according to any one of claims 2 or 3, characterized in that the extract or the composition are intended to inhibit the expression of metallo-proteinase type 1. 5. Use in a composition or for the preparation of a composition of at least one extract of at least one plant of the genus Salvia, the extract or composition being intended to treat menopausal skin disorders. 6. Use in a composition or for the preparation of a composition of at least one extract of at least one plant of the genus Salvia, the extract or composition being intended to fight against lines and wrinkles. 7. Use in a composition or for the preparation of a composition of at least one extract of at least one plant of the genus Salvia, the extract or composition being intended to fight against skin withered. 8. Use in a composition or for the preparation of a composition of at least one extract of at least one plant of the genus Salvia, the extract or the composition being intended to fight against soft skin. 9. Use in a composition or for the preparation of a composition of at least one extract of at least one plant of the genus Salvia, the extract or composition being intended to fight against thinned skin. <Desc / Clms Page number 16>  10. Use in a composition or for the preparation of a composition of at least one extract of at least one plant of the genus Salvia, the extract or the composition being intended to combat the lack of elasticity and / or tone of the skin. 11. Use in a composition or for the preparation of a composition of at least one extract of at least one plant of the genus Salvia, the extract or the composition being intended to fight against internal damage to the skin resulting from a exposure to ultraviolet radiation. 12. Use according to claim 11, the extract or the composition being intended to fight against collagen degradations. 13. Use according to any one of the preceding claims, characterized in that the plant material is derived from whole plant or part of plant such as leaves, stems, flowers, petals, roots or even dedifferentiated cells . 14. Use according to claim 13, characterized in that the plant material is derived from the leaves. 15. Use according to any one of the preceding claims, characterized in that the plant material is derived from at least one plant of the genus Salvia grown in vivo or from in vitro culture. 16. Use according to claim 15, characterized in that the plant material is derived from at least one plant of the genus Salvia grown in vivo. 17. Use according to any one of the preceding claims, characterized in that the extract is an aqueous extract. 18. Use according to any one of the preceding claims, characterized in that the extract is used in an amount representing 0.00001% to 20% of the total weight of the composition. 19. Use according to the preceding claim, characterized in that the extract is used in an amount representing from 0.0001% to 10% of the total weight of the composition. <Desc / Clms Page number 17>  20. Use according to any one of the preceding claims, characterized in that the composition is intended for administration by the oral route. 21. Use according to the preceding claim, characterized in that the composition is in the form of an oral solution, a syrup, a tablet, a capsule, a capsule or a nutritional food or a nutritional supplement. 22. A cosmetic skin treatment method for treating the cutaneous signs of aging, characterized in that it is applied to the skin, to the hair, and / or to the mucous membranes, or when a composition is ingested cosmetic composition comprising at least one extract of at least one plant of the genus Salvia. 23. Cosmetic skin treatment method for treating wrinkles and fine lines, wilted skin, mole skin, thinned skin, lack of elasticity and / or tone of the skin or any internal changes in the skin which do not systematically result in a modified external appearance, such as, for example, any internal damage to the skin, particularly collagen, resulting from exposure to ultraviolet radiation, characterized by the fact that it is applied to the skin, to the hair, and / or on the mucous membranes, or that a cosmetic composition comprising at least one extract of at least one plant of the genus Salvia is ingested.