FR2539622A1 - METHOD AND APPARATUS FOR STIMULATING HAIR GROWTH AND REGENERATION - Google Patents

Abstract

COMPOSITION FOR THE TREATMENT OF SCALP BY ELECTROPHORESIS, IN PARTICULAR CONSTITUTE OF AN AMINO-VINYL PHOTOSENSITIZER SOLUTION. APPARATUS FOR THE APPLICATION OF THIS COMPOSITION: IT CONSISTS OF TWO OSCILLATORS PRODUCING A SQUARE WAVE SUPERIMPOSED ON A POTENTIAL FOR ACTION.

Description

The present invention relates to a method and apparatus

  of electrotherapy; it aims, more

  particularly, an electrophoresis method for stimulating

  the growth and regeneration of hair, and a device for the implementation of this method. Various chemical and physical means, including electrotherapeutic, have been tried to remedy the alopecia A fast and complete treatment is however very difficult in many cases of alopecia, because their

  causes are still unclear Even when the treatment

  is effective, several weeks or years are always

  necessary for complete treatment Etiology

  complicated with alopecia did not establish a tea-

defined therapeutic.

  Recent studies on alopecia have led to encouraging results, in that the symptoms of persistent alopecia seem very similar to those of temporary alopecia and that changes in scalp conditions affect growth. and the

  regeneration of hair from follicles In general

  neral hair follicles only disappear

at the death of the individual.

  It can therefore be expected that some means of stimulating hair follicles

  substantial growth and regeneration of hair.

  Electrotherapeutic methods are described in patent applications, in which the follicles

  hair is stimulated by application to the leather

  a low-frequency direct current or a modulated DC current, for example a square wave or a tooth of

  saw, to accelerate the growth and regeneration of

  want. A typical process is described in

  U.S. Patent No. 3,872,859 to A Sylvester et al.

  It involves successively placing two electrodes, spacers

  from each other, against predetermined surfaces of the scalp region overlying the top of the skull; passing an electric current between these electrodes, the current being in the form of a low frequency wave in the range of 200 to 3000 Hz, with an electrical potential sufficient to stimulate these organs; and massage

  the scalp in the area of at least one of the electrodes

of.

  Although the electrotherapeutic aspect of the procedure

  deserves the interest, this process is insufficient to treat

  completely alopecia, for the following reasons:

  the application of such a continuous current on the skin provoking

  that inevitably a deep electrical burn and the undesirable formation of vesicles, bubbles and redness of the skin; the continuous application of this current is very unpleasant for the patient, because of its inadequate waveform, so that the treatment can not be continued until a satisfactory result is obtained;

  in addition, the electrotherapeutic methods of stimulating

  The growth of hair regeneration growth using a low frequency wave only has limited effectiveness.

  There is therefore a strong need for a practical process

  effective and efficient way of stimulating growth and

  hair generation As will appear on reading

  of the description which follows, the present invention

to this need.

  The subject of the present invention is an electrical process

  trophoretic, or iontophoretic, stimulation of the

  regeneration of the hair The invention provides

  also a massage of the skin by electric means.

  It also aims at the elimination, by

of the deposited pigment.

  The present invention also aims at a device

  tif for the implementation of such an electrother-

2539622.

3.

  which is of economic manufacture and design

  safe and trouble-free, so that it can be easily and safely used at home,

for self-treatment.

  According to the invention, these objects are achieved by the method which consists in applying to the scalp a charge by means of a low frequency square wave, on which is superimposed a wave of action potential.

  in two phases, to perform the electrophoresis of a

posed aminovynilic.

  The present invention is based on the observation

  electrophoresis of certain aminoviral compounds

  in the scalp, by means of a low frequency square wave, on which is superimposed a wave of

  two-phase action potential, causes a

  markable and regeneration of hair, without effects

  undesirable side effects, and that this electrophoresis,

  If it is applied to the skin in other parts of the body, for example the hands, legs or face, it substantially eliminates the pigment deposited therein, in particular the melamine pigment. The present invention is also based on

  the discovery that electrophoresis can be performed eco-

  nomically and securely by means of a com-

  taking: an oscillator that generates a square wave of 0.5 to 2 Hz, having a zero potential and a positive potential; another oscillator that generates a potential wave of

  two-phase action, with a pulse width of the

  dre from 1/200 to 1/600 second and a pulse interval of the order of 1/20 to 1/30 second; a power supply

  electrical circuits in energy; a first electro

  of, controlled by the oscillators; a second electro-

  from, maintained at the earthing potential at which the

  the first electrode are reported; an electrical circuit

  that to superimpose the action potential to the square wave 2539622 i

  low frequency; and a link switch of each

any oscillators on the stack.

  The preferred aminovinyl compounds in the

  Some photosensitizing dyes which have a common vinyl structure having one or more N-heteroaromatic rings at positions 1 and / or 2- are present, one or more of the aromatic rings being quaternary ions associated with a halide. These photosensitization dyes generally have strong bands of

  absorption of ultraviolet light and have special colors

  As aminovinyl dyes, photosensitive

  available on the market, L-iodide (3-heptylmethyl-4-thiazolin-2-ylidene) is available.

  -2-hylidene propenylene-3 '2,21-bis (heptyl-3-methyl)

  4-thiazolinium thioxide) (KANKOH-SO N 101); iodide (3-heptyl-4-methyl-2-thiazolin-2-ylidene) -2-methine D-3-heptyl-4-methyl-thiazolinium

(KANKOH-SO N 201);

  i (2-Bromo-2-pyridyl) -2-aminovinyl-1-ethyl-2-picotinium (KANKOH-50 N 301) iodide and (2-anilino-vinyl) -2-dimethyl-3,4-oxazolium iodide

(KANKOH-50 N 401)

  which are products of the Japanese Research Institute for Photosensitizing Dyes, in Okayama, Japan,

  and all of which are advantageously usable in the invention.

  tion. Before its use, the aminovinyl compound is generally dissolved in an alcoholic saline solution,

  in order to obtain a concentration of the order of

  tens to several hundred milligrams per liter The solution is then applied directly to

  the sick part of the body, or it is used by ab-

  sorption in or on a spool type electrode

  Then, the low-frequency square wave, in super-

  position with a wave of two-phase action potential, is applied on the part to be treated, in order to perform

  electrophoresis of the compound through the skin.

  The action potential wave, according to the present

  the invention must be a short two-phase pulse.

  its, having a waveform similar to that obtained by

  usually used in normal muscles during ex-

  tension and contraction of the muscle: the brief impulse

  ve alternates symmetrically, along the time axis, with

  a pulse width of the order of 10 milliseconds.

  The brief impulse transmits the information, which is

  in the stimulated parts of the muscle, in the brain

through the nerves.

  The square wave of low frequency to be applied can be generated by superposition of a wave of two-phase action potential, of a frequency of the order of 50

  at 300 Hz, on a square wave, comprising

  zero and positive current, a frequency of

  0.5 to 2 Hz When seeking to remove the deposited pigment, the superimposed square wave may be replaced by a alternating two-phase action potential wave at a positive potential value slightly below its wavelength, with a pulse interval of the order of 1/10 to 1/200 second and a pulse width

  of the order of 1/100 to 1/500 second.

  The superimposed square wave can be applied, in a general way, to the place to be treated, under several

  volts at several tens of volts approximately, or 0.1 milliam-

  father to 10 milliamperes, but it must be carefully

  within the above limits, in order to meet the

  Disadvantages of the diseased part or the symptom of the patient Depending on the symptoms of the patient, a treatment may be suitably performed as follows: for example, a small portion of a saline solution of the aminovinyl compound in methanol, at a concentration of order of 10 to

  1000 mg / l is applied directly to the

  ter, or absorbed in a roller type electrode

  spongy, before treatment.

  squared, for several minutes or more

  if tens of minutes, at the place to be treated, in order to perform the electrophoresis of the compound The progress of the effect of the treatment, that is to say the growth and regeneration of the hair or the elimination, is monitored. deposited pigment, and the procedures described above may in

  consequently be performed once or several times a day.

  One or more agents or nutrients,

  for example acetyl choline, steroids, sugar, protein, a-

  minoacid or vitamin, can be introduced at the same time as the aminovinyl compound Electrophoresis of the aminovinyl compound in combination with certain sugars or proteins, for example glucose or serum, can produce

a more effective treatment.

  The invention will be better understood in the light

  description of its mode of implementation, not limited

  tative, shown in the accompanying drawings in which Fig 1 is a block diagram of the device for stimulating the growth and regeneration of hair,

according to the present invention.

  Fig 2 illustrates the waveforms of the device, Fig 1 (a) and Fig 1 (b) showing the waveforms of

  output generated by the first and second oscilla-

  respectively, and Fig 2 (c) representing the waveform of the superimposed square wave;

  Fig 3 is a diagram of the electrical circuit of the dis-

  according to the invention, comprising two multivibration

  1 and 2 as first and second oscillators; FIG. 4 is a diagram of the electrical circuit of a

  another device according to the invention, comprising a bone

  a blocking capacitor 1 and a multivibrator 2 as first and second oscillators respectively;

  Fig 5 illustrates the connection of the two

  low frequency and high reliability

  chronoscope, used in test 2.

  In FIGS. 1-5, 1 denotes the first bone

  cillateur; 2, the second oscillator; 3 and 4, the

  nes; 5, the spongy roller type electrode; 6, the mass electrode; C, a capacitor; R, a resistor;

  S, a switch; Sy, a synchronoscope; T, a trans-

  former; E, a listener; and Tr, a transistor.

  Figure 1 illustrates a device according to

  the invention, comprising a first oscillator 1 which generates

  a two-phase action potential, with an interval

  the pulse of the order of 1/200 to 1/600 second and a pulse width of the order of 1/20 to 1/300 second,

  as shown in Figure 2 (a) and a second

  2 which generates a square wave comprising

  zero and positive current, a frequency of

  in the order of 0.5 to 2 Hz, as shown in Figure 2 (b).

  The square wave is superimposed on the wave of potential

  action, to obtain the low frequency wave that

  appropriate for the intended purpose (Figure 2c).

  The positive region of the superimposed square wave, ie from K to L, and another region alternating on either side of the zero-volt line, that is, from L to M , actually produce the electrophoresis of the compound

  aminovinyl and the electric massage of the skin, respec-

tively.

  The superimposed square wave thus obtained is

  on the skin, using a spongy roller type electrode (the first electrode) and a second electrode

  electrode 6 (maintained at ground potential serving as

  for the signals from the first electrode), through

to both terminals 3 and 4.

  FIG. 3 illustrates the electrical circuit of a device according to the invention. In this device, a blocking oscillator and a multivibrator are used.

2539622 3

  as first and second oscillators, to generate the

  action potential wave and the square wave respectively.

  The action potential wave is sent through the

  primary bearing of a transformer T at terminal 3 to which the spongy roller type electrode is connected. On the other hand, the square wave of low frequency with zero and positive current components, with a frequency of the order of 0.5 to 2 Hz, generated by the multivibrator,

  is introduced into an emitter-follower circuit,

  as a transistor T Fr 4, to reduce its output impedance

  The output current is then sent to the winding

  of the transformer, in which it is

  given to the wave of action potential The battery B 2 excites

the transistor.

  Figure 4 illustrates another compliant device

  to the invention, in which two tube multivibrators

  are used as the first and second oscilla-

tors.

  The square wave generated by the first multivariate

  1, a frequency of the order of 50 to 300 Hz, is modified by the circuit comprising a capacitor C 9 and a resistor R 15, to obtain a wave of potential of

  action, as shown in Figure 2 (a), which is

  superimposed on the low frequency square wave, of a frequency of the order of 0.5 to 2 Hz, generated by the other multivibrator 2, in order to obtain the desired superimposed square wave, as shown in FIG. Figure 2 (c) The current

  superimposed is suitable for the electrophoresis of the amino

vinylic according to the invention.

  The superimposed square wave is sent to two elec-

  trodes 5 and 6, via the two terminals 3 and 4.

  The capacitor C 12 charges and discharges repeatedly

  the square wave current generated by the second

  multivibrator 2, through the resistance R 19 to remove

  Thus, the waveform of the output current of the capacitor C 12 becomes substantially square.

  The remarkable therapeutic effects of

  are highlighted by the tests below.

TEST 1

  Aminovinyl Compounds In the device according to the invention, as represented in FIG. 3, the pulse interval and the pulse width of the action potential, generated by the blocking oscillator (the first oscillator), the frequency of the multivibrator (the second oscillator) is set to 1 Hz. Before the test, the hair is removed from

  groups of rabbits by applying cream of silver

  in four places, about 5 cm each

  cun, in their dorsal region.

  rest for a short time, then wash them thoroughly.

  in such a way as not to injure the animals.

  The first group of animals is treated with acetyl choline in glycerin, or a solution

  of aminovinyl compound prepared by dissolving

  Kankoh-so salt solution N O 301 or 401 so as to ob-

  maintain a concentration of 0.05% The mixture is supplemented with 0.5 part of 95% alcohol and then a charge is applied by the superimposed square wave, for 10 minutes, by means of the device according to the invention, at a rate of of an operation

  10 minutes each day (group A).

  The second group of animals is treated in the same way as above, except that in place of the superimposed wave the animal is applied to a tooth wave.

  with a frequency of 3 000 Hz, by means of the

usual (group B).

  The third group of animals is also treated

2 c 39622 l

  with each preparation, but without subsequent application

  re of the electric wave (group C).

  The fourth group of animals is used as a control and it does not receive any application of product or load by electric wave (group D). During the course of the test, the hairs

  shaved 10, 20 and 30 days after the start of the experiment.

  microscope the lengths of 100 hairs cut The average results are shown in

table 1.

  The results confirm that growth and regeneration

  hair growth is slightly accelerated by applying

  cation of the aminovinyl compound on the skin and are sen-

  greatly enhanced by the combined load of the

  which is superimposed on a wave of potential ac-

  The results also confirm that the stimulation of

  obtained by the combination of the compound and the superimposed square wave is greater than that obtained

  by means of the sawtooth wave according to the prior art.

TABLE 1.

  Kankoh-so agent Kankoh-so Acetyl Treatment NO 301 No. 401 choline days 10.2 9.5 5.1 A (mm) 20 days 26.7 22.2 13.2 days 42.4 34.1 17.2 B (mm) days days days days C (mm) 20 days 30 days 6.5 17.2, 3, 8 16.4 22.0, 3 16.2 24.8 4.5 13.1 19.2 4, 9 12.1, 7 4.1, 8, 2539622 Table 1 (Continued) Kankoh-so agent Kankoh-so Acetyl Treatment NI 301 N 401 choline days 2.9 1.6 3.0 D (mmn) 20 days 8.7 9.9 5, 8 days 15.8 16.6 11.6 Note: A, group in which electrophoresis of the product

  O 10 is carried out by means of the device according to the invention.

  tion; B, a group in which the electrophoresis of the product is carried out by means of a usual device; C, a group in which only the product is applied to the skin, without then charging by electric current;

  and D, a group in which no application is made

  product or electrophoresis.

TEST 2

  Square wave superimposed Two oscillators and 2 low frequency, of

  high reliability, are connected to a Sy synchronoscope.

  as shown in FIG. 5 o The superimposed current is

  applied, through two terminals 3 and 4,

  be the positive and negative electrodes 5 and 6 and ob-

  serve simultaneously the waveform with the synchronosco-

pe

  The preferable frequency ranges of oscilla-

  1 and 2 are determined in the same way as in 1 '.

  Test i, except that their frequencies are modified as shown in Table 2 and in that as photosensitizing aminovynilic dye 200 mg / l is used

  of Kankoh-so N O 301 in an alcoholic saline solution.

  The results are shown in Table 2. These results confirm that the highest possible growth stimulation and hair regeneration are obtained when the square wave frequencies, generated by the oscillator-1, and the potential

  the action generated by the oscillator 2 are 1 Hz

  around 50 Hz at 200 Hz, respectively.

TABLE 2

  N Frequency Frequency A B Osc (Hz) Osc 2 (Hz) (average in mm) i 10 days 20 days 30 days

1.1 0.5 0.2

* - * 50 2.5 3.2 3.8

1,100 0.1 1.9 4.1 5.2

500 1.0 2.2 2.0

1,000 0.8 1,1 2,2

0.3 0.9 1.7

3.1 6.2 7.8

2,100 0.5 2.7 5.8 8.1

500 1.9 3.5 2.8

1000 1.6 1.4 0.0

1.8 1.6 2.1

4.5 9.3 14.2

3,100 1.0 5.1 11.1 17.4

500 2.8 4.5 8.5

1,000 0,8 1,1 1,2

-1.1 0.1 0.2

3.1 6.7 7.8

4,100 1.5 3.2 5.8 -6.3

500 1.2 2.3 3.2

1,000 0.8 0.2 0.7

0.1 0.2 1.0

2.1 3.6 4.0

100 2.0 1.9 2.8 3.1

500 0.8 1.5 1.1

1,000 0.7 0.4 0.2

0.5 0.2 0.3

1.1 2.1 2.2

6,100 3.0 0.9 0.1 1.5

500 0.5 0.2 0.9

1,000 0.0 0.3 0.7

cut, ob-

  that which Note: A indicates the average length of the bristles held with the process according to the invention, and B

is obtained with the indicator (mm).

2539622 I

  On the basis of the above confirmations, the pre-

  electrophoretic process has been applied to various

  patients with alopecia, using Kankoh-

  N o 301 and 401 Satisfactory cures are obtained

  in cases of alopecia areata, alopecia praematura,

  alopecia pityrodes and alopecia atrophicans.

  The high efficiency of the process according to the invention

  can be interpreted as follows: comparatively

  usual products, the aminovinyl compound is

  more effective way to stimulate growth and regeneration

  hair ration; the action potential, superimposed on the square wave, actually acts on the nerves to make

  disappear their fatigue and also accelerate the metabo-

skin.

  It is therefore possible to carry out a treatment

  ment and effective physical therapy.

  It is understood that modifications of detail may be made in the form and implementation

  of the method and apparatus according to the invention, without

shot from the frame of it.

2539622 2

Claims (8)

claims   1 Composition for the treatment of the hair chevere by electrophoresis, characterized in that it is constituted   by an amino-vinyl photosensitizer solution.   Composition according to Claim 1, characterized   in that the solution is alcoholic, saline,   preferably 10 to 1000 ppm of amino-vinyl compound.   3 Composition according to claim 1 or 2,   characterized in that the photosensitizer is:   β-iodide (3-heptyl-4-methylthiazolin-2-ylidene-2) -   2-propenylenyl-3'-2,2 '-bis (3-heptyl-4-methylthiazolinium) (KANKOH-SO N 101); 3-(3-heptyl-4-methyl-2-thiazolin-2-ylidene) methine; 2-heptyl-3-methyl-thiazolinium (KANKOH-SO N 201);   Z (5-bromo-2-pyridyl) aminovinyl] -2-ethyl-1-picolinium-2} -6-iodide (KANKOH-SO-N-301) and (2-anilino-vinyl) -2-dimethyl-3,4-oxazolium iodide (KANKOH-SO N 401).   4 Composition according to one of the preceding claims   characterized in that the solution also contains   nutrition agent or nutrient   particular aceto-choline, steroid, sugar, protein, amino noacid or vitamin.   Apparatus for applying the following composition   one of claims 1 to 4, comprising oscilla-   characterized in that a first oscillator (1) is provided for generating a two-phase action potential, with a pulse interval of the order of 1/200 to 1/600 of a second, while a second oscillator (2) produces a square wave with components of zero and positive current, with a frequency of the order of 0.5 to 2 Hz, the square wave being superimposed on the action potential to give a wave of low frequency.   Apparatus according to Claim 5, characterized in that the pulse width of the first oscillator   is of the order of 1/20 to 1/300 of a second.   Apparatus according to claim 5 or 6, characterized   in that the two oscillators are multivitrators.   Apparatus according to claim 5 or 6, characterized   rised in that the first oscillator is an oscillator   blocking, the second being a multivibrator.