FR2450263A1 - Coupling protein to latex particles having epoxy gps. - for use in immunological analysis esp. pregnancy testing - Google Patents
Coupling protein to latex particles having epoxy gps. - for use in immunological analysis esp. pregnancy testingInfo
- Publication number
- FR2450263A1 FR2450263A1 FR7905248A FR7905248A FR2450263A1 FR 2450263 A1 FR2450263 A1 FR 2450263A1 FR 7905248 A FR7905248 A FR 7905248A FR 7905248 A FR7905248 A FR 7905248A FR 2450263 A1 FR2450263 A1 FR 2450263A1
- Authority
- FR
- France
- Prior art keywords
- esp
- gps
- latex particles
- monomer
- pregnancy testing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
- C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
- C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
- C12N11/087—Acrylic polymers
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
Abstract
Coupling of protein (I) having a reactive gp. which includes a labile H atom., to latex particles of dia. 0.15-1.5 mu comprises reacting at pH 7-9 to form covalent bonds between (I) and free epoxy gps. in the lates. Unfixed (I) is then removed. The latex particles comprise a core, made by (co)polymerising >=1 'hard' monomer, and an outer sheel made by copolymerising >=1 'hard' monomer and an ethylenically unsatd. monomer having free epoxy gps. Both core and shell may include a 'soft' comonomer. Specifically (I) includes NH2 gps., esp. it is human chorionic gonadotropin (HCG), insulin or an enzyme, and the latex is esp. styrene-glycidyl methacrylate. The conjugates are useful in antigen-antibody testing, esp. pregnancy testing. Severe conditions which might denature (I) are avoided; the particles are of uniform size distribution, so are partic. suitable for automated analysis.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR7905248A FR2450263A1 (en) | 1979-02-28 | 1979-02-28 | Coupling protein to latex particles having epoxy gps. - for use in immunological analysis esp. pregnancy testing |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR7905248A FR2450263A1 (en) | 1979-02-28 | 1979-02-28 | Coupling protein to latex particles having epoxy gps. - for use in immunological analysis esp. pregnancy testing |
Publications (2)
Publication Number | Publication Date |
---|---|
FR2450263A1 true FR2450263A1 (en) | 1980-09-26 |
FR2450263B1 FR2450263B1 (en) | 1985-03-15 |
Family
ID=9222593
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FR7905248A Granted FR2450263A1 (en) | 1979-02-28 | 1979-02-28 | Coupling protein to latex particles having epoxy gps. - for use in immunological analysis esp. pregnancy testing |
Country Status (1)
Country | Link |
---|---|
FR (1) | FR2450263A1 (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0234083A2 (en) * | 1986-02-25 | 1987-09-02 | Tosoh Corporation | Carrier for a biologically active component for immunoassay or enzymatic reaction |
EP0291390A1 (en) * | 1987-05-11 | 1988-11-17 | Rhone-Poulenc Chimie | Process for the preparation of polymer particles or of a latex made of polymer particles having on their surface amphiphile molecules with inorganic or reactive groups |
CN113677993A (en) * | 2019-03-29 | 2021-11-19 | 积水医疗株式会社 | Immunoassay reagent and immunoassay method |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2077297A1 (en) * | 1970-01-23 | 1971-10-22 | Exploaterings Ab Tbf | |
US3844392A (en) * | 1971-11-02 | 1974-10-29 | Bosch Gmbh Robert | One-way clutch arrangement |
FR2321520A1 (en) * | 1975-08-22 | 1977-03-18 | Rhone Poulenc Ind | LATEX FROM POLYMERS WITH CN-GROUPS |
FR2332287A1 (en) * | 1975-11-22 | 1977-06-17 | Behringwerke Ag | COMBINATIONS WITH BIOLOGICAL ACTIVITY, THEIR PREPARATION PROCESS |
US4070348A (en) * | 1973-07-25 | 1978-01-24 | Rohm Gmbh | Water-swellable, bead copolymer |
-
1979
- 1979-02-28 FR FR7905248A patent/FR2450263A1/en active Granted
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2077297A1 (en) * | 1970-01-23 | 1971-10-22 | Exploaterings Ab Tbf | |
US3844392A (en) * | 1971-11-02 | 1974-10-29 | Bosch Gmbh Robert | One-way clutch arrangement |
US4070348A (en) * | 1973-07-25 | 1978-01-24 | Rohm Gmbh | Water-swellable, bead copolymer |
FR2321520A1 (en) * | 1975-08-22 | 1977-03-18 | Rhone Poulenc Ind | LATEX FROM POLYMERS WITH CN-GROUPS |
FR2332287A1 (en) * | 1975-11-22 | 1977-06-17 | Behringwerke Ag | COMBINATIONS WITH BIOLOGICAL ACTIVITY, THEIR PREPARATION PROCESS |
Non-Patent Citations (1)
Title |
---|
DIE ANGEWANDTE MAKROMOLEKULARE CHEMIE, vol. 72, no. 72, octobre 1978 * |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0234083A2 (en) * | 1986-02-25 | 1987-09-02 | Tosoh Corporation | Carrier for a biologically active component for immunoassay or enzymatic reaction |
EP0234083A3 (en) * | 1986-02-25 | 1989-11-08 | Tosoh Corporation | Carrier for a biologically active component for immunoassay or enzymatic reaction |
EP0291390A1 (en) * | 1987-05-11 | 1988-11-17 | Rhone-Poulenc Chimie | Process for the preparation of polymer particles or of a latex made of polymer particles having on their surface amphiphile molecules with inorganic or reactive groups |
FR2615192A1 (en) * | 1987-05-11 | 1988-11-18 | Rhone Poulenc Chimie | PROCESS FOR THE PREPARATION OF PARTICLES OF POLYMER OR LATEX CONSISTING OF PARTICLES OF POLYMER COMPRISING, IMPLANTED AT THEIR SURFACE, AMPHIPHILIC MOLECULES CARRYING IONOGENIC OR REACTIVE GROUPS |
CN113677993A (en) * | 2019-03-29 | 2021-11-19 | 积水医疗株式会社 | Immunoassay reagent and immunoassay method |
Also Published As
Publication number | Publication date |
---|---|
FR2450263B1 (en) | 1985-03-15 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
ST | Notification of lapse |