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ES8703934A1 - Un metodo para detectar una secuencia de polinucleotidos - Google Patents

Un metodo para detectar una secuencia de polinucleotidos

Info

Publication number
ES8703934A1
ES8703934A1 ES546536A ES546536A ES8703934A1 ES 8703934 A1 ES8703934 A1 ES 8703934A1 ES 546536 A ES546536 A ES 546536A ES 546536 A ES546536 A ES 546536A ES 8703934 A1 ES8703934 A1 ES 8703934A1
Authority
ES
Spain
Prior art keywords
interest
polynucleotide sequence
dectection
composition
technique
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES546536A
Other languages
English (en)
Other versions
ES546536A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Enzo Biochem Inc
Original Assignee
Enzo Biochem Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Enzo Biochem Inc filed Critical Enzo Biochem Inc
Publication of ES546536A0 publication Critical patent/ES546536A0/es
Publication of ES8703934A1 publication Critical patent/ES8703934A1/es
Expired legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6832Enhancement of hybridisation reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00Chemistry: analytical and immunological testing
    • Y10T436/14Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333Saccharide [e.g., DNA, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

METODO PARA DETECTAR UNA SECUENCIA DE POLINUCLEOTIDO. COMPRENDE: A) PROPORCIONAR UNA COMPOSICION; B) FORMAR UNA CADENA SENCILLA MEDIANTE PORCIONES DE SECUENCIA DE POLINUCLEOTIDO DE INTERES Y LAS SECUENCIAS DE POLINUCLEOTIDO DE INTERES; C) CONFORMAR UNA PORCION DE LA COMPOSICION EN CADENA SENCILLA; D) PONER EN CONTACTO A LA SECUENCIA DE POLINUCLEOTIDO DE INTERES Y LAS SECUENCIAS DE POLINUCLEOTIDOS NO INTERES CON LA COMPOSICION PARA HIBRIDARLOS; Y E) DETECTAR LA SECUENCIA DE POLINUCLEOTIDO DE INTERES MEDIANTE EL PRIMER MARCADOR DETECTABLE. LA COMPOSICION ESTA FORMADA POR: UNA PRIMERA SECUENCIA DE POLINUCLEOTIDO, CAPAZ DE HIBRIDAR CON LA SECUENCIA DE POLINUCLEOTIDO DE INTERES Y ESTA MARCADO CON EL PRIMER MARCADOR DETECTABLE; UNA SEGUNDA SECUENCIA DE POLINUCLEOTIDO IDENTICA A LA SECUENCIA DE INTERES Y MARCADA CON EL PRIMER MARCADOR DETECTABLE Y UNA TERCERA SECUENCIA DE POLINUCLEOTIDO MARCADA CON UN SEGUNDO MARCADOR DETECTABLE.
ES546536A 1984-08-30 1985-08-29 Un metodo para detectar una secuencia de polinucleotidos Expired ES8703934A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/646,171 US4755458A (en) 1984-08-30 1984-08-30 Composition and method for the detection of the presence of a polynucleotide sequence of interest

Publications (2)

Publication Number Publication Date
ES546536A0 ES546536A0 (es) 1987-03-01
ES8703934A1 true ES8703934A1 (es) 1987-03-01

Family

ID=24592052

Family Applications (1)

Application Number Title Priority Date Filing Date
ES546536A Expired ES8703934A1 (es) 1984-08-30 1985-08-29 Un metodo para detectar una secuencia de polinucleotidos

Country Status (11)

Country Link
US (1) US4755458A (es)
EP (1) EP0173339B1 (es)
JP (1) JPH0634758B2 (es)
AT (1) ATE71982T1 (es)
AU (1) AU597874B2 (es)
CA (1) CA1260368A (es)
DE (1) DE3585249D1 (es)
DK (1) DK391985A (es)
ES (1) ES8703934A1 (es)
IL (1) IL76143A (es)
NO (1) NO853408L (es)

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DE3585249D1 (de) 1992-03-05
ATE71982T1 (de) 1992-02-15
DK391985D0 (da) 1985-08-29
ES546536A0 (es) 1987-03-01
AU597874B2 (en) 1990-06-14
EP0173339A3 (en) 1987-12-23
JPS6165163A (ja) 1986-04-03
DK391985A (da) 1986-03-01
EP0173339A2 (en) 1986-03-05
AU4646685A (en) 1986-03-06
IL76143A0 (en) 1985-12-31
IL76143A (en) 1990-02-09
NO853408L (no) 1986-03-03
EP0173339B1 (en) 1992-01-22
CA1260368A (en) 1989-09-26
US4755458A (en) 1988-07-05

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