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ES8106015A1 - Procedimiento para la obtencion de un microorganismo termo- estable y enzima proteolitica preparada a partir del mismo - Google Patents

Procedimiento para la obtencion de un microorganismo termo- estable y enzima proteolitica preparada a partir del mismo

Info

Publication number
ES8106015A1
ES8106015A1 ES494778A ES494778A ES8106015A1 ES 8106015 A1 ES8106015 A1 ES 8106015A1 ES 494778 A ES494778 A ES 494778A ES 494778 A ES494778 A ES 494778A ES 8106015 A1 ES8106015 A1 ES 8106015A1
Authority
ES
Spain
Prior art keywords
organism
proteolytic enzyme
micro
thermo
processes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES494778A
Other languages
English (en)
Other versions
ES494778A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Development Finance Corp of New Zealand
Original Assignee
Development Finance Corp of New Zealand
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Development Finance Corp of New Zealand filed Critical Development Finance Corp of New Zealand
Publication of ES8106015A1 publication Critical patent/ES8106015A1/es
Publication of ES494778A0 publication Critical patent/ES494778A0/es
Granted legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • C12N1/205Bacterial isolates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/10Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14Enzymes or microbial cells immobilised on or in an inorganic carrier
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/814Enzyme separation or purification
    • Y10S435/815Enzyme separation or purification by sorption
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/8215Microorganisms
    • Y10S435/822Microorganisms using bacteria or actinomycetales

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Inorganic Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

METODO DE OBTENCION DE UNA ENZIMA PROTEOLITICA A PARTIR DE UN MICROORGANISMO TERMOESTABLE. SE AISLA UNA MUESTRA DE SOLUCION PROCEDENTE DE UN ESTANQUE CALIENTE CON UN CONTENIDO BAJO EN SULFUROS Y CON PH DE 7,8, CONTENIENDO EL MICROORGANISMO ADECUADO. SE CULTIVA LA MUESTRA EN UN MEDIO LIQUIDO QUE CONTIENE 0,1-0,3 POR 100 DE SALES DE ALLEN, 0,1 A 100 DE EXTRACTO DE LEVADURA Y 0,1 POR 100 DE TRIPTICASA. SE LE MANTIENE A TEMPERATURA DE 65-85 GC Y PH 7,2-8,2 UNAS 18 HORAS. SE CENTRIFUGA EL CULTIVO Y SE SOMETE AL SOBRENADANTE A CROMATOGRAFIA POR AFINIDAD. EL CONCENTRADO DE LA ENZIMA OBTENIDO SE ELUYE CON UN EQUILIBRADOR DE ELUCIONADO A PH 8,1. DE APLICACION EN LA INDUSTRIA DE ALIMENTACION, FERMENTACION, FARMACEUTICA Y DE PIENSOS ALIMENTICIOS.
ES494778A 1979-08-08 1980-08-08 Procedimiento para la obtencion de un microorganismo termo- estable y enzima proteolitica preparada a partir del mismo Granted ES494778A0 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
NZ19124679 1979-08-08

Publications (2)

Publication Number Publication Date
ES8106015A1 true ES8106015A1 (es) 1981-07-16
ES494778A0 ES494778A0 (es) 1981-07-16

Family

ID=19918876

Family Applications (1)

Application Number Title Priority Date Filing Date
ES494778A Granted ES494778A0 (es) 1979-08-08 1980-08-08 Procedimiento para la obtencion de un microorganismo termo- estable y enzima proteolitica preparada a partir del mismo

Country Status (13)

Country Link
US (2) US4442214A (es)
EP (1) EP0024182B1 (es)
JP (1) JPS5664786A (es)
AU (1) AU541968B2 (es)
CA (1) CA1177766A (es)
DE (1) DE3071593D1 (es)
DK (1) DK149867C (es)
ES (1) ES494778A0 (es)
GB (1) GB2055850B (es)
IE (1) IE51742B1 (es)
IL (1) IL60787A (es)
IS (1) IS1191B6 (es)
ZA (1) ZA804833B (es)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4879235A (en) * 1985-11-29 1989-11-07 The Japanese Research And Development Association For Bioreactor System Process for producing protease by cultivating a protease-producing mold in a liquid medium
US5192677A (en) * 1986-05-08 1993-03-09 Cornell Research Foundation Inc. Alkali and heat stable protease from thermomonospora fusca
US5283189A (en) * 1987-02-09 1994-02-01 Research Development Corporation Of Japan β-galactosidase from Thermus sp
DE3834550A1 (de) * 1988-10-11 1990-04-19 Basf Ag Proteolytisches enzym, seine herstellung und verwendung
US5215907A (en) * 1989-02-24 1993-06-01 Oklahoma Medical Research Foundation Thermostable acid protease from sulfolobus acidocaldarius
US5173403A (en) * 1989-02-24 1992-12-22 Oklahoma Medical Research Foundation Thermostable acid protease from sulfolobus acidocaldarius and gene
DK145890D0 (da) * 1990-06-15 1990-06-15 Novo Nordisk As Thermostabil protease
US5643777A (en) * 1990-06-15 1997-07-01 Novo Nordisk A/S Thermostable protease from thermococcus
DK145990D0 (da) * 1990-06-15 1990-06-15 Novo Nordisk As Thermostabil protease
DK146090D0 (da) * 1990-06-15 1990-06-15 Novo Nordisk As Thermostabil protease
DE4228776A1 (de) * 1992-08-28 1994-03-03 Bosch Siemens Hausgeraete Vorrichtung zum Anreichern von Wasser mit CO¶2¶-Gas zur Erzeugung von karbonisiertem Wasser
WO1995005477A1 (en) * 1993-08-12 1995-02-23 University Of Maryland Thermostable alkaline metalloprotease produced by a hyphomonas, and preparation thereof
AU7108698A (en) * 1997-04-11 1998-11-11 Thermogen, Inc. Stable biocatalysts for ester hydrolysis
NZ511680A (en) * 2001-05-14 2004-07-30 Univ Waikato Method for preparing nucleic acid or DNA samples and a DNA extraction process using thermophilic proteases
US7148049B2 (en) 2002-04-02 2006-12-12 Roche Molecular Systems, Inc. Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity
ATE532875T1 (de) * 2004-06-17 2011-11-15 Zygem Corp Ltd Reinigungsverfahren und ihre verwendungen
NL1028064C2 (nl) * 2005-01-18 2006-07-19 Vhp Ugchelen Bv Authenticatiewerkwijze en -systeem voor het authenticeren van veiligheidsdocumenten en veiligheidsdocument.
US8815315B2 (en) * 2007-04-10 2014-08-26 Kemin Industries, Inc. Use of a multi-protease system to improve the protein digestibility of animal feeds containing vegetable meals
WO2010019898A1 (en) * 2008-08-14 2010-02-18 Zygem Corporation Limited Temperature controlled nucleic-acid detection method suitable for practice in a closed-system
US8828238B2 (en) 2012-05-29 2014-09-09 Industrial Technology Research Instiute Thermophilic bacterium and uses of extracellular proteins therefrom
US8968687B2 (en) 2012-05-29 2015-03-03 Industrial Technology Research Institute Method for recycling metals
US10281374B2 (en) * 2015-11-04 2019-05-07 Diagnostic Biosystems Method of pretreatment of biological samples for an analyte-staining assay method

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3713983A (en) * 1970-07-06 1973-01-30 Kikkoman Shoyu Co Ltd Method for preparing a thermostable and alkali-stable protease
JPS5345395B2 (es) * 1972-03-18 1978-12-06
US3796635A (en) * 1972-12-26 1974-03-12 Monsanto Co Process for the preparation of heat resistant neutral protease enzyme
DE2434672A1 (de) * 1973-07-26 1975-04-30 Kreidl Chemico Physical Kg Verfahren und vorrichtung zur herstellung von enzymatischen umsetzungsprodukten niedermolekularer stoffe, insbesondere zur konzentrationsbestimmung niedermolekularer biologischer stoffe durch enzymatische umsetzung
SU644796A1 (ru) * 1976-06-09 1979-01-30 Всесоюзный научно-исследовательский институт генетики и селекции промышленных микроорганизмов Способ очистки протеолитических ферментов
JPS5413295A (en) * 1977-06-30 1979-01-31 Matsushita Electric Works Ltd Display unit
JPS54132295A (en) * 1978-04-05 1979-10-15 Mitsubishi Chem Ind Ltd Preparation of phosphofructokinase

Also Published As

Publication number Publication date
DK149867C (da) 1987-04-06
EP0024182A3 (en) 1981-04-22
EP0024182A2 (en) 1981-02-25
JPS5664786A (en) 1981-06-02
GB2055850B (en) 1983-08-24
IE801679L (en) 1981-02-08
IE51742B1 (en) 1987-03-18
IL60787A0 (en) 1980-10-26
DK149867B (da) 1986-10-13
ZA804833B (en) 1981-08-26
US4442214A (en) 1984-04-10
DK339680A (da) 1981-02-09
IS2577A7 (is) 1981-02-09
IS1191B6 (is) 1985-06-18
IL60787A (en) 1984-08-31
GB2055850A (en) 1981-03-11
AU541968B2 (en) 1985-01-31
AU6120080A (en) 1981-02-12
US4480036A (en) 1984-10-30
ES494778A0 (es) 1981-07-16
CA1177766A (en) 1984-11-13
DE3071593D1 (en) 1986-06-12
EP0024182B1 (en) 1986-05-07

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