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ES485047A1 - Support Matrices for Immobilized Enzymes - Google Patents

Support Matrices for Immobilized Enzymes

Info

Publication number
ES485047A1
ES485047A1 ES485047A ES485047A ES485047A1 ES 485047 A1 ES485047 A1 ES 485047A1 ES 485047 A ES485047 A ES 485047A ES 485047 A ES485047 A ES 485047A ES 485047 A1 ES485047 A1 ES 485047A1
Authority
ES
Spain
Prior art keywords
support
copolymeric
immobilized enzymes
aminopolystyrene
pores
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES485047A
Other languages
Spanish (es)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Honeywell UOP LLC
Original Assignee
UOP LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US05/951,949 external-priority patent/US4206259A/en
Priority claimed from US05/951,948 external-priority patent/US4193910A/en
Application filed by UOP LLC filed Critical UOP LLC
Publication of ES485047A1 publication Critical patent/ES485047A1/en
Expired legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14Enzymes or microbial cells immobilised on or in an inorganic carrier
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08FMACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F8/00Chemical modification by after-treatment
    • C08F8/04Reduction, e.g. hydrogenation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Inorganic Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

Organic-inorganic support matrices for immobilized enzymes comprise a solid, porous, inorganic, water insoluble support and a copolymeric material resulting from the reaction of aminopolystyrene and a bifunctional monomer. The copolymeric material is substantially entrapped in the pores of the support and contains functionalized pendent groups to which an enzyme may be coupled to form the immobilized enzyme conjugate. The matrix may be prepared by depositing a salt of aminopolystyrene within the pores of the solid support from an aqueous solution at a pH less than 7 after which the resulting composite is then reacted with an excess of a bifunctional reactive monomer thus forming the copolymeric organic material in situ.
ES485047A 1978-10-16 1979-10-16 Support Matrices for Immobilized Enzymes Expired ES485047A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US05/951,949 US4206259A (en) 1978-10-16 1978-10-16 Support matrices for immobilized enzymes
US05/951,948 US4193910A (en) 1978-10-16 1978-10-16 Preparation of support matrices for immobilized enzymes

Publications (1)

Publication Number Publication Date
ES485047A1 true ES485047A1 (en) 1980-05-16

Family

ID=27130323

Family Applications (1)

Application Number Title Priority Date Filing Date
ES485047A Expired ES485047A1 (en) 1978-10-16 1979-10-16 Support Matrices for Immobilized Enzymes

Country Status (7)

Country Link
CA (1) CA1128917A (en)
DE (1) DE2941881C2 (en)
DK (1) DK436379A (en)
ES (1) ES485047A1 (en)
FR (1) FR2453898A1 (en)
GB (1) GB2033399B (en)
IT (1) IT1125483B (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0062968B2 (en) * 1981-03-18 1991-07-10 FUJIREBIO KABUSHIKI KAISHA also trading as FUJIREBIO INC. Support material for use in serological testing and process for the production thereof
DE3130924A1 (en) * 1981-08-05 1983-02-17 Röhm GmbH, 6100 Darmstadt SURFACE-BASED SYSTEMS FOR FIXING SUBSTRATES CONTAINING NUCLEOPHILE GROUPS
CN113061600A (en) * 2021-03-05 2021-07-02 宁波泓森生物科技有限公司 Preparation method of immobilized threonine aldolase, immobilized threonine aldolase and application
CN114540332B (en) * 2022-03-01 2023-07-21 江苏恰瑞生物科技有限公司 Preparation method and application of uricase immobilized resin

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3860486A (en) * 1973-05-24 1975-01-14 Owens Illinois Inc Insolubilizing enzymes with polystyrene derivatives
GB1485122A (en) * 1973-08-06 1977-09-08 Nat Res Dev Biologically active matrices
US4001085A (en) * 1973-09-10 1977-01-04 Owens-Illinois, Inc. Immobilization of enzymes on an inorganic matrix
GB1485123A (en) * 1974-07-25 1977-09-08 Nat Res Dev Support for biologically active material
PT64790B (en) * 1975-02-18 1977-07-07 Uop Inc Immobilized enzyme conjugates
US4141857A (en) * 1976-04-30 1979-02-27 Uop Inc. Support matrices for immobilized enzymes

Also Published As

Publication number Publication date
GB2033399A (en) 1980-05-21
DE2941881C2 (en) 1983-11-10
DE2941881A1 (en) 1980-04-17
FR2453898A1 (en) 1980-11-07
CA1128917A (en) 1982-08-03
IT7926535A0 (en) 1979-10-16
FR2453898B1 (en) 1983-11-18
IT1125483B (en) 1986-05-14
GB2033399B (en) 1982-11-24
DK436379A (en) 1980-04-17

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 19990601