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ES249137A1 - Reagent and method for determining the coagulability of blood - Google Patents

Reagent and method for determining the coagulability of blood

Info

Publication number
ES249137A1
ES249137A1 ES0249137A ES249137A ES249137A1 ES 249137 A1 ES249137 A1 ES 249137A1 ES 0249137 A ES0249137 A ES 0249137A ES 249137 A ES249137 A ES 249137A ES 249137 A1 ES249137 A1 ES 249137A1
Authority
ES
Spain
Prior art keywords
cephalin
factor
plasma
reagent
bovine
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES0249137A
Other languages
Spanish (es)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ARNOR ORWEN PAUL
Original Assignee
ARNOR ORWEN PAUL
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ARNOR ORWEN PAUL filed Critical ARNOR ORWEN PAUL
Publication of ES249137A1 publication Critical patent/ES249137A1/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/56—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/96—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood or serum control standard
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2496/00—Reference solutions for assays of biological material
    • G01N2496/05—Reference solutions for assays of biological material containing blood cells or plasma

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Neurosurgery (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A reagent for testing the coagulability of blood during treatment with anticoagulent agents comprises thromboplastin in optimal concentration having a Quick's thromboplastin time of at least 35 seconds with normal human plasma in admixture with cephalin or a cephalin substitute, e.g. lecithin. Bovine plasma from which prothrombin, Stuart-factor, proconvertin and antihemophilia-B-factor have been removed and which contains high concentrations of antihemophilia-A and -C-factors, Hagemanfactor, proaccelarin and proconvertin, and calcium chloride may also be present, the latter in an amount sufficient to give the optimal concentration of Ca\sR\sR ions for coagulation after the addition of test plasmas. The reagent may be freeze-dried to give a stable, solid preparation which may be reconstituted before use. The prothrombin may be prepared by known procedures from animal (e.g. bovine, horse, swine) brain and the cephalin from human brain. The bovine plasma may be obtained by the treatment thereof with barium sulphate followed by dialysis to remove oxalate and the carbonic acid/bicarbonate buffer system and adjustment of the pH to from 6-8.ALSO:A reagent for testing the coagulability of blood during treatment with anticoagulent agents comprises thromboplastin in optional concentration having a Quick's thromboplastin time of at least 35 seconds with normal human plasma in admixture with cephalin or a cephalin substitute e.g. lecithin. Bovine plasma from which prothrombin, Stuart-factor, proconvertin and antihaemophilia-b -factor have been removed and which contains high concentrations of antihaemophilia-A and -C-factors, Hageman-factor, proaccelarin and proconvertin, and calcium chloride may also be present, the latter in amounts sufficient to give the optimal concentration of CA++ ions for coagulation after the addition of test plasma. The reagent may be freeze-dried to give a stable, solid preparation which may be reconstituted before use. The prothrombin may be prepared by known methods from animal (e.g. bovine, horse, swine) brain and the cephalin from human brain. The bovine plasma may be obtained by the treatment thereof with barium sulphate followed by dialysis to remove oxalate and the carbonic acid/bicarbonate buffer system and adjustment of the pH to from 6 to 8.
ES0249137A 1958-05-16 1959-05-02 Reagent and method for determining the coagulability of blood Expired ES249137A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
NO12810458 1958-05-16

Publications (1)

Publication Number Publication Date
ES249137A1 true ES249137A1 (en) 1959-11-16

Family

ID=19908436

Family Applications (1)

Application Number Title Priority Date Filing Date
ES0249137A Expired ES249137A1 (en) 1958-05-16 1959-05-02 Reagent and method for determining the coagulability of blood

Country Status (7)

Country Link
US (1) US3179567A (en)
CH (1) CH408283A (en)
DE (1) DE1189763B (en)
ES (1) ES249137A1 (en)
FR (1) FR1223856A (en)
GB (1) GB917012A (en)
NL (2) NL123584C (en)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3293134A (en) * 1961-09-08 1966-12-20 Warner Lambert Pharmaceutical Diagnostic reagent composition for determining blood coagulation factors and method of use
US3395210A (en) * 1964-02-07 1968-07-30 Warner Lambert Pharmaceutical Lyophilized diagnostic reagent for the determination of blood coagulation factors
US3486981A (en) * 1965-03-15 1969-12-30 Roy E Speck Substances relating to testing of blood-coagulation
US3228841A (en) * 1965-06-10 1966-01-11 Warner Lambert Pharmaceutical Diagnostic reagent composition for determining blood coagulation factors and method of use
US3880714A (en) * 1973-07-18 1975-04-29 Warner Lambert Co Diagnostic reagent
US3947378A (en) * 1974-12-23 1976-03-30 Warner-Lambert Company Adsorbed plasma
US4002739A (en) * 1975-02-18 1977-01-11 Warner-Lambert Company Soluble collagen
US4038147A (en) * 1976-06-15 1977-07-26 Becton, Dickinson And Company Method for detecting endotoxins
DE2915310A1 (en) * 1979-04-14 1980-10-30 Behringwerke Ag NEW PARTIAL THROMBOPLASTINE AND THEIR USE
DE3150596A1 (en) * 1981-12-21 1983-06-30 Boehringer Mannheim Gmbh, 6800 Mannheim METHOD FOR PRODUCING TISSUE HROMBOPLASTIN
DE3407280A1 (en) * 1984-02-28 1985-09-19 Gödecke AG, 1000 Berlin TEST SET FOR DETERMINING THE ACTIVATED PARTIAL THROMBOPLASTIN TIME (PTT) WITH INCREASED HEPARINE SENSITIVITY
DE3413311A1 (en) * 1984-04-09 1985-10-17 Behringwerke Ag, 3550 Marburg REAGENT FOR DETERMINING THROMBOPLASTIN TIME
US4946775A (en) * 1985-09-05 1990-08-07 Yin E Thye Composition, kit and method for assaying heparin and a method for making the composition
US4948724A (en) * 1985-09-05 1990-08-14 Yin E Thye Composition, kit and method for assaying heparin and a method for making the composition
US4851336A (en) * 1985-09-05 1989-07-25 Yin E Thye Composition, kit, and method for assaying heparinano a method for making the composition
US4755461A (en) * 1986-04-17 1988-07-05 Bio/Data Corporation Tableted blood plasma microconcentrated thromboplastin coagulation reagent
US6203816B1 (en) * 1990-11-13 2001-03-20 Corvas International, Inc. Tissue factor based prothrombin time reagent
US5314695A (en) * 1990-11-13 1994-05-24 Corvas International, Inc. Tissue factor based prothrombin time reagent
AU744287B2 (en) * 1990-11-13 2002-02-21 Corvas International, Inc. Tissue factor based prothrombin time reagent
US6248353B1 (en) 1999-12-10 2001-06-19 Dade Behring Inc. Reconstitution of purified membrane proteins into preformed liposomes
US6596543B2 (en) 2001-03-22 2003-07-22 Dade Behring Inc. Use of liposomes of defined composition and size for the preparation of prothrombin time reagents
IS2809B (en) * 2011-03-08 2012-10-15 Haskoli Islands Method for monitoring anticoagulant therapy
EP3694322B1 (en) 2017-10-09 2024-07-03 Terumo BCT Biotechnologies, LLC Lyophilization container and method of using same
US11609042B2 (en) 2019-03-14 2023-03-21 Terumo Bct Biotechnologies, Llc Multi-part lyophilization container and method of use
CN112481355B (en) * 2020-11-16 2023-05-30 武汉市长立生物技术有限责任公司 Liquid prothrombin time determination kit and preparation method thereof

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2687980A (en) * 1948-09-15 1954-08-31 Ernest W Blanchard Thromboplastin product and method for preparing same
US2847350A (en) * 1954-05-27 1958-08-12 Ortho Pharma Corp Preparation of highly active thromboplastin

Also Published As

Publication number Publication date
NL123584C (en)
DE1189763B (en) 1965-03-25
GB917012A (en) 1963-01-30
CH408283A (en) 1966-02-28
NL238873A (en)
US3179567A (en) 1965-04-20
FR1223856A (en) 1960-06-21

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