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ES2193843B1 - PROCEDURE FOR REVERSIBLE INACTIVATION BY HEAT, AND IN VIVO, OF PROTEINS WITH BIOLOGICAL ACTIVITY. - Google Patents

PROCEDURE FOR REVERSIBLE INACTIVATION BY HEAT, AND IN VIVO, OF PROTEINS WITH BIOLOGICAL ACTIVITY.

Info

Publication number
ES2193843B1
ES2193843B1 ES200101595A ES200101595A ES2193843B1 ES 2193843 B1 ES2193843 B1 ES 2193843B1 ES 200101595 A ES200101595 A ES 200101595A ES 200101595 A ES200101595 A ES 200101595A ES 2193843 B1 ES2193843 B1 ES 2193843B1
Authority
ES
Spain
Prior art keywords
procedure
heat
proteins
biological activity
vivo
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES200101595A
Other languages
Spanish (es)
Other versions
ES2193843A1 (en
Inventor
Ana Isabel Rojas Gonzalez
Juan Bautista Jordano Fraga
M. Concepcion Almoguera Antolinez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consejo Superior de Investigaciones Cientificas CSIC
Original Assignee
Consejo Superior de Investigaciones Cientificas CSIC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consejo Superior de Investigaciones Cientificas CSIC filed Critical Consejo Superior de Investigaciones Cientificas CSIC
Priority to ES200101595A priority Critical patent/ES2193843B1/en
Priority to PCT/ES2002/000332 priority patent/WO2003004522A1/en
Publication of ES2193843A1 publication Critical patent/ES2193843A1/en
Application granted granted Critical
Publication of ES2193843B1 publication Critical patent/ES2193843B1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Procedimiento para la inactivación reversible por calor, e in vivo, de proteínas con actividad biológica. El procedimiento permitiría que una proteína a la que se le añaden nuevos aminoácidos, pueda perder de forma transitoria su actividad biológica: enzimática, reguladora, o cualquier otro tipo de función que esta posea. Los aminoácidos añadidos proceden de HSPs (Heat Shock Proteins). La inactivación ocurre tras un tratamiento breve con calor (choque térmico), tras el cual la actividad de la proteína modificada se recupera con un cierto retraso (entre las 3 y 24 horas después del tratamiento). Esta herramienta podría utilizarse, por ejemplo, para el diseño de nuevos sistemas de expresión inducible de genes en plantas transgénicas.Procedure for reversible inactivation by heat, and in vivo, of proteins with biological activity. The procedure would allow a protein to which new amino acids are added, may temporarily lose its biological activity: enzymatic, regulatory, or any other type of function it possesses. The added amino acids come from HSPs (Heat Shock Proteins). Inactivation occurs after a brief heat treatment (thermal shock), after which the activity of the modified protein is recovered with a certain delay (between 3 and 24 hours after treatment). This tool could be used, for example, for the design of new inducible gene expression systems in transgenic plants.

ES200101595A 2001-07-06 2001-07-06 PROCEDURE FOR REVERSIBLE INACTIVATION BY HEAT, AND IN VIVO, OF PROTEINS WITH BIOLOGICAL ACTIVITY. Expired - Fee Related ES2193843B1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
ES200101595A ES2193843B1 (en) 2001-07-06 2001-07-06 PROCEDURE FOR REVERSIBLE INACTIVATION BY HEAT, AND IN VIVO, OF PROTEINS WITH BIOLOGICAL ACTIVITY.
PCT/ES2002/000332 WO2003004522A1 (en) 2001-07-06 2002-07-03 Method for the reversible in vivo heat inactivation of proteins with biological activity

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES200101595A ES2193843B1 (en) 2001-07-06 2001-07-06 PROCEDURE FOR REVERSIBLE INACTIVATION BY HEAT, AND IN VIVO, OF PROTEINS WITH BIOLOGICAL ACTIVITY.

Publications (2)

Publication Number Publication Date
ES2193843A1 ES2193843A1 (en) 2003-11-01
ES2193843B1 true ES2193843B1 (en) 2005-03-01

Family

ID=8498337

Family Applications (1)

Application Number Title Priority Date Filing Date
ES200101595A Expired - Fee Related ES2193843B1 (en) 2001-07-06 2001-07-06 PROCEDURE FOR REVERSIBLE INACTIVATION BY HEAT, AND IN VIVO, OF PROTEINS WITH BIOLOGICAL ACTIVITY.

Country Status (2)

Country Link
ES (1) ES2193843B1 (en)
WO (1) WO2003004522A1 (en)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5187267A (en) * 1990-06-19 1993-02-16 Calgene, Inc. Plant proteins, promoters, coding sequences and use
US5922929A (en) * 1997-05-02 1999-07-13 University Of Maryland At Baltimore County Thermotolerance enhancing protein
AU5926700A (en) * 1999-07-08 2001-01-30 Stressgen Biotechnologies Corporation Induction of a th1-like response in vitro
GB9920194D0 (en) * 1999-08-27 1999-10-27 Advanced Biotech Ltd A heat-stable thermostable DNA polymerase for use in nucleic acid amplification

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
COCA, M.A. et al. "Differential regulation of small heat-shock genes in plants: analysis of water-stress-inducible and developmentally activated sunflower promoter". PLANT MOLECULAR BIOLOGY, 1996, Vol. 31, páginas 863-876, todo el documento. *
SILVERIO, J.M. et al. "Reversible inactivation and binding to mitochondria of nitrate reductase by heat shock in the yeast Hansenula anomala". FEBS LETTERS, Marzo 1993, Vol. 318, nº 2, páginas 153-156, todo el documento. *

Also Published As

Publication number Publication date
ES2193843A1 (en) 2003-11-01
WO2003004522A1 (en) 2003-01-16

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