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ES2180263T3 - Metodo de ensayo luminiscente homogeneo basado en transferencia de energia. - Google Patents

Metodo de ensayo luminiscente homogeneo basado en transferencia de energia.

Info

Publication number
ES2180263T3
ES2180263T3 ES99660099T ES99660099T ES2180263T3 ES 2180263 T3 ES2180263 T3 ES 2180263T3 ES 99660099 T ES99660099 T ES 99660099T ES 99660099 T ES99660099 T ES 99660099T ES 2180263 T3 ES2180263 T3 ES 2180263T3
Authority
ES
Spain
Prior art keywords
energy
luminescent
energy transfer
homogeneum
luminiscent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES99660099T
Other languages
English (en)
Inventor
Martti Latva
Ilkka Hemmila
Kaj Blomberg
Pertti Hurskainen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wallac Oy
Original Assignee
Wallac Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wallac Oy filed Critical Wallac Oy
Application granted granted Critical
Publication of ES2180263T3 publication Critical patent/ES2180263T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La invención es un ensayo de bioafinidad a base de transferencia de energía luminiscente que comprende un primer grupo marcado con un compuesto de donación de energía (donante) y un segundo grupo de aceptación de energía (receptor) , donde el donante es un quelato de lantánido luminiscente que tiene una larga duración de vida en estado excitado y el receptor es o un compuesto luminiscente que tiene una corta duración de vida en estado excitado o un compuesto no luminiscente. Se mide el incremento o la reducción, respectivamente de la transferencia de energía del donante a receptor, resultante del acortamiento o del alargamiento respectivamente, de la distancia entre dichos grupos.
ES99660099T 1998-07-17 1999-06-03 Metodo de ensayo luminiscente homogeneo basado en transferencia de energia. Expired - Lifetime ES2180263T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/116,763 US5998146A (en) 1998-07-17 1998-07-17 Homogeneous luminescence assay method based on energy transfer

Publications (1)

Publication Number Publication Date
ES2180263T3 true ES2180263T3 (es) 2003-02-01

Family

ID=22369056

Family Applications (1)

Application Number Title Priority Date Filing Date
ES99660099T Expired - Lifetime ES2180263T3 (es) 1998-07-17 1999-06-03 Metodo de ensayo luminiscente homogeneo basado en transferencia de energia.

Country Status (4)

Country Link
US (1) US5998146A (es)
EP (1) EP0973036B1 (es)
DE (1) DE69902724T2 (es)
ES (1) ES2180263T3 (es)

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US9261460B2 (en) 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
US6760104B2 (en) 2001-07-20 2004-07-06 Grigoriy Gomelskiy Apparatus, method, and system for analyzing samples using triboluminescent technology
DE10137530A1 (de) * 2001-08-01 2003-02-13 Presens Prec Sensing Gmbh Anordnung und Verfahren zur Mehrfach-Fluoreszenzmessung
DE10153829A1 (de) 2001-11-05 2003-05-28 Bayer Ag Assay basierend auf dotierten Nanoteilchen
DE10159579A1 (de) * 2001-12-05 2003-06-18 Applera Deutschland Gmbh Heterogenes Lumineszenz-Nachweisverfahren in Kavitätenstrukturen
ATE439368T1 (de) * 2001-12-19 2009-08-15 Univ Chicago Schnellreifende fluoreszierende proteine und verfahren zu deren anwendung
US7371582B2 (en) * 2002-01-23 2008-05-13 Boditechmed Inc. Lateral flow quantitative assay method and strip and laser-induced fluorescence detection device therefor
US9353405B2 (en) 2002-03-12 2016-05-31 Enzo Life Sciences, Inc. Optimized real time nucleic acid detection processes
US20030232394A1 (en) * 2002-06-12 2003-12-18 Aventis Pharma Deutschland Gmbh High throughput screening method and assay system for determining the interaction between C-reactive protein and components binding to it
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DE10259677B4 (de) * 2002-12-18 2008-01-24 Büddefeld, Jürgen, Prof. Dr.-Ing. Sonden zum Nachweis von Nukleinsäuren, insbesondere zum Nachweis pathogener Keime in Lebensmitteln
ES2254044T3 (es) 2002-12-26 2010-12-30 ZAKRYTOE AKTSIONERNOE OBSCHESTVO " EVROGEN" Proteinas fluorescentes procedentes de especies de copepodos y procedimientos para usarlas.
FI20030460A0 (fi) * 2003-03-28 2003-03-28 Tero Soukka Homogeeninen määritysmenetelmä, joka perustuu luminesenssienergiasiirtoon
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US7727752B2 (en) 2003-07-29 2010-06-01 Life Technologies Corporation Kinase and phosphatase assays
WO2005033709A1 (en) * 2003-10-01 2005-04-14 Wallac Oy Improved homogeneous time-resolved energy transfer assay
US7250298B2 (en) * 2004-04-07 2007-07-31 The University Of Chicago Monomeric red fluorescent proteins
US20060147954A1 (en) * 2004-10-19 2006-07-06 Wallac Oy Novel probe and its use in bioaffinity assays
WO2006042912A1 (en) * 2004-10-22 2006-04-27 Wallac Oy Highly sensitive homogeneous assay based on anti-stokes' shift fret measurement
WO2007052102A2 (en) * 2005-11-04 2007-05-10 Evrogen, Jsc Modified green fluorescent proteins and methods for using same
US8563703B2 (en) 2006-01-25 2013-10-22 Evrogen IP Joint Stock Company Fluorescent proteins and methods for using same
DE602007009498D1 (de) 2006-01-25 2010-11-11 Evrogen Ip Neue fluoreszenzproteine und verfahren zur verwendung davon
EP2511266B1 (en) 2006-07-10 2019-09-04 The Regents of The University of California Luminescent 1-hydroxy-2-pyridinone chelates of lanthanides
US8173800B2 (en) 2006-08-15 2012-05-08 The Regents Of The University Of California Luminescent macrocyclic lanthanide complexes
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US8679749B2 (en) * 2007-11-01 2014-03-25 The University Of Chicago Red fluorescent proteins with enhanced bacterial expression, increased brightness and reduced aggregation
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WO2009134897A1 (en) * 2008-04-29 2009-11-05 Burnham Institute For Medical Research E3-independent ubiquitinylation assay
DE202008009713U1 (de) * 2008-06-26 2009-08-06 InBio Prof. Jürgen Büddefeld Dr. Peter Klauth Prof. Manfred Rietz GbR (vertretungsberechtigter Gesellschafter: Dr. Peter Klauth, 41189 Mönchengladbach) Abstandsgesteuerte Energietransfer-Farbstoffkomplexe
US9273059B2 (en) 2009-08-24 2016-03-01 Lumiphore, Inc. Macrocyclic HOPO chelators
FI20090306A0 (fi) 2009-08-25 2009-08-25 Qret Technologies Oy Erotusvapaa määritysmenetelmä
US20110189088A1 (en) * 2009-12-24 2011-08-04 Lumiphore, Inc. Radiopharmaceutical complexes
US8834846B2 (en) 2010-05-06 2014-09-16 Bruker Biospin Corporation Fluorescent NIRF activatable probes for disease detection
EP2598649A1 (en) * 2010-07-31 2013-06-05 Advanced Biomedical Limited Method, reagent, and apparatus for detecting a chemical chelator
WO2012037361A2 (en) 2010-09-15 2012-03-22 Endacea, Inc. Methods of use and kit for measurement of lipopolysaccharide with a time resolved fluorescence based assay
US11453652B2 (en) 2013-03-15 2022-09-27 Lumiphore, Inc. Di-macrocycles
CN104181310A (zh) * 2014-07-30 2014-12-03 美艾利尔（上海）诊断产品有限公司 一种检测β-hCG含量的时间分辨免疫荧光试剂盒及其制备方法和应用
WO2016207124A1 (en) 2015-06-25 2016-12-29 F. Hoffmann-La Roche Ag Cell based assay for determining antibody or ligand binding and function
CN110088625B (zh) 2016-12-21 2023-11-03 豪夫迈·罗氏有限公司 用于确定抗体或配体结合和功能的测定法
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CN112946272B (zh) * 2021-01-28 2021-12-24 无锡壹闪生物科技有限公司 一种核基质蛋白22的快速检测试剂盒及其应用

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Also Published As

Publication number Publication date
DE69902724T2 (de) 2003-01-09
US5998146A (en) 1999-12-07
EP0973036B1 (en) 2002-09-04
EP0973036A2 (en) 2000-01-19
EP0973036A3 (en) 2000-03-08
DE69902724D1 (de) 2002-10-10

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