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ES2162132T3 - Polimerasa de adn termoestable procedente de thermoanaerobacter thermohydrosulfuricus y enzimas mutantes, de actividad exonucleasa suprimida asi obtenidos. - Google Patents

Polimerasa de adn termoestable procedente de thermoanaerobacter thermohydrosulfuricus y enzimas mutantes, de actividad exonucleasa suprimida asi obtenidos.

Info

Publication number
ES2162132T3
ES2162132T3 ES96944875T ES96944875T ES2162132T3 ES 2162132 T3 ES2162132 T3 ES 2162132T3 ES 96944875 T ES96944875 T ES 96944875T ES 96944875 T ES96944875 T ES 96944875T ES 2162132 T3 ES2162132 T3 ES 2162132T3
Authority
ES
Spain
Prior art keywords
dna polymerase
thermo
supplied
exonuclease activity
mutant enzymes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES96944875T
Other languages
English (en)
Inventor
Joseph A Mamone
Maria Davis
Dan Sha
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Global Life Sciences Solutions USA LLC
Original Assignee
Amersham Pharmacia Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Pharmacia Biotech Inc filed Critical Amersham Pharmacia Biotech Inc
Application granted granted Critical
Publication of ES2162132T3 publication Critical patent/ES2162132T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

SE DESCRIBE UNA ADN POLIMERASA ENZIMATICAMENTE ACTIVA O UN FRAGMENTO DE LA MISMA, QUE PRESENTA AL MENOS UN 80 % DE HOMOLOGIA EN SU SECUENCIA DE AMINOACIDOS CON AL MENOS UNA SECUENCIA DE 40 AMINOACIDOS CONTIGUA DE LA ADN POLIMERASA DE THERMOANAEROBACTER THERMOHYDROSULFURICUS, Y ENZIMAS MUTANTES EN LAS CUALES SE HA ELIMINADO LA ACTIVIDAD EXONUCLEASA.
ES96944875T 1995-12-15 1996-12-13 Polimerasa de adn termoestable procedente de thermoanaerobacter thermohydrosulfuricus y enzimas mutantes, de actividad exonucleasa suprimida asi obtenidos. Expired - Lifetime ES2162132T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US868895P 1995-12-15 1995-12-15

Publications (1)

Publication Number Publication Date
ES2162132T3 true ES2162132T3 (es) 2001-12-16

Family

ID=21733102

Family Applications (1)

Application Number Title Priority Date Filing Date
ES96944875T Expired - Lifetime ES2162132T3 (es) 1995-12-15 1996-12-13 Polimerasa de adn termoestable procedente de thermoanaerobacter thermohydrosulfuricus y enzimas mutantes, de actividad exonucleasa suprimida asi obtenidos.

Country Status (8)

Country Link
US (1) US5744312A (es)
EP (1) EP0866868B1 (es)
JP (1) JP4073038B2 (es)
AT (1) ATE203056T1 (es)
CA (1) CA2240334C (es)
DE (1) DE69613856T2 (es)
ES (1) ES2162132T3 (es)
WO (1) WO1997021821A1 (es)

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US7556958B1 (en) * 1997-04-08 2009-07-07 The Rockefeller University Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof
EP0921196A1 (en) 1997-12-02 1999-06-09 Roche Diagnostics GmbH Modified DNA-polymerase from carboxydothermus hydrogenoformans and its use for coupled reverse transcription and polymerase chain reaction
CA2324248A1 (en) * 1998-03-18 1999-09-23 Amersham Pharmacia Biotech Inc. Thermostable dna polymerase from thermoanaerobacter thermohydrosulfuricus
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
ATE371022T1 (de) 1998-06-17 2007-09-15 Ge Healthcare Bio Sciences Fy7 polymerase
US6406891B1 (en) 1998-09-28 2002-06-18 Board Of Regents, The University Of Texas System Dual RT procedure for cDNA synthesis
US6238868B1 (en) * 1999-04-12 2001-05-29 Nanogen/Becton Dickinson Partnership Multiplex amplification and separation of nucleic acid sequences using ligation-dependant strand displacement amplification and bioelectronic chip technology
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
KR100527265B1 (ko) 1999-09-13 2005-11-09 뉴젠 테크놀로지스 인코포레이티드 폴리뉴클레오티드 서열의 선형 등온 증폭을 위한 방법 및조성물
US6692918B2 (en) 1999-09-13 2004-02-17 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
US7846733B2 (en) * 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
EP1356094B1 (en) 2000-06-26 2010-01-13 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
KR20000072141A (ko) * 2000-08-04 2000-12-05 김유삼 신규한 호열성 단백질 분해효소의 ｄｎａ 서열 및그로부터 유래된 단백질
ATE475720T1 (de) * 2000-12-13 2010-08-15 Nugen Technologies Inc Methoden und zusammensetzungen zur generierung einer vielzahl von kopien von nukleinsäuresequenzen und methoden zur detektion derselben
US6794141B2 (en) 2000-12-22 2004-09-21 Arcturus Bioscience, Inc. Nucleic acid amplification
WO2002072773A2 (en) * 2001-03-09 2002-09-19 Nugen Technologies, Inc. Methods and compositions for amplification of rna sequences
ZA200210369B (en) * 2001-03-09 2004-07-08 Nugen Technologies Inc Methods and compositions for amplification or RNA sequences.
JP2004523243A (ja) 2001-03-12 2004-08-05 カリフォルニア インスティチュート オブ テクノロジー 非同期性塩基伸長によってポリヌクレオチド配列を分析するための方法および装置
KR20010079080A (ko) * 2001-06-12 2001-08-22 김유삼 신규한 dna 중합효소의 dna 서열 및 그로부터 유래된 단백질
US20070020622A1 (en) * 2001-09-14 2007-01-25 Invitrogen Corporation DNA Polymerases and mutants thereof
IL162207A0 (en) * 2001-12-20 2005-11-20 Amersham Biosciences Corp Nucleic acid labeling by thermoanaerocter thermohydrosulfuricus dna polymeasei variants
US20030180741A1 (en) 2001-12-21 2003-09-25 Holly Hogrefe High fidelity DNA polymerase compositions and uses therefor
WO2003079667A1 (en) * 2002-03-15 2003-09-25 Arcturus Bioscience, Inc. Improved nucleic acid amplification
DE10315640A1 (de) * 2003-04-04 2004-10-14 Ignatov, Konstantin Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung
WO2004092418A2 (en) * 2003-04-14 2004-10-28 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
US7169560B2 (en) * 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
CA2557177A1 (en) 2004-02-19 2005-09-01 Stephen Quake Methods and kits for analyzing polynucleotide sequences
US20060046258A1 (en) * 2004-02-27 2006-03-02 Lapidus Stanley N Applications of single molecule sequencing
US20050260609A1 (en) * 2004-05-24 2005-11-24 Lapidus Stanley N Methods and devices for sequencing nucleic acids
EP1766090B1 (en) 2004-05-25 2011-04-27 Helicos Biosciences Corporation Methods for nucleic acid immobilisation
US7476734B2 (en) 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
GB2416352B (en) * 2004-07-21 2009-01-28 Bioline Ltd A method for performing the hot start of enzymatic reactions
JP2008522628A (ja) * 2004-12-11 2008-07-03 サイトジェニックス， インコーポレイテッド 高品質核酸のセルフリー生合成及びその使用
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US7482120B2 (en) 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
CA2621267A1 (en) * 2005-09-07 2007-03-15 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
ATE537251T1 (de) * 2005-10-06 2011-12-15 Lucigen Corp Temperaturstabile virale polymerasen und verwendungsverfahren
US20090305248A1 (en) * 2005-12-15 2009-12-10 Lander Eric G Methods for increasing accuracy of nucleic acid sequencing
WO2007117331A2 (en) * 2005-12-22 2007-10-18 Ge Healthcare Bio-Sciences Corp. Novel dna polymerase from thermoanaerobacter tengcongenesis
US20080309926A1 (en) * 2006-03-08 2008-12-18 Aaron Weber Systems and methods for reducing detected intensity non uniformity in a laser beam
US7397546B2 (en) * 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
EP3796002B1 (en) 2006-07-14 2023-11-22 The Regents of The University of California Cancer biomarkers and methods of use thereof
US20090203531A1 (en) * 2008-02-12 2009-08-13 Nurith Kurn Method for Archiving and Clonal Expansion
US7846666B2 (en) 2008-03-21 2010-12-07 Nugen Technologies, Inc. Methods of RNA amplification in the presence of DNA
EP3508854A1 (en) 2010-04-27 2019-07-10 The Regents of The University of California Cancer biomarkers and methods of use thereof
US9777319B2 (en) 2012-06-29 2017-10-03 General Electric Company Method for isothermal DNA amplification starting from an RNA template
US11543411B2 (en) 2014-12-05 2023-01-03 Prelude Corporation DCIS recurrence and invasive breast cancer
JP2022500504A (ja) 2018-09-14 2022-01-04 プレリュード コーポレーションPrelude Corporation 浸潤性乳癌のリスクを有する対象の治療の選択方法
NL2022993B1 (en) 2019-04-23 2020-11-02 Synvolux Ip Bv Methods and compositions for isothermal DNA amplification
EP4083204A1 (en) 2021-04-30 2022-11-02 Wageningen Universiteit Gene wide randomization
US20240110221A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Methods of modulating clustering kinetics

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US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5405774A (en) * 1986-08-22 1995-04-11 Hoffmann-La Roche Inc. DNA encoding a mutated thermostable nucleic acid polymerase enzyme from thermus species sps17
US4889818A (en) * 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5173411A (en) * 1987-01-14 1992-12-22 President And Fellows Of Harvard College Method for determining the nucleotide base sequence of a DNA molecule
JP2968585B2 (ja) * 1989-12-22 1999-10-25 エフ．ホフマン ― ラ ロシュ アーゲー 高温度逆転写酵素
DE69131321T2 (de) * 1990-09-28 1999-12-16 F. Hoffmann-La Roche Ag, Basel Mutationen in der 5'-3'-exonukleaseaktivität thermostabiler dna-polymerasen
ATE143057T1 (de) * 1994-10-17 1996-10-15 Harvard College Dns polymerase mit veränderter nukleotid- bindungstelle

Also Published As

Publication number Publication date
EP0866868A1 (en) 1998-09-30
DE69613856D1 (de) 2001-08-16
JP4073038B2 (ja) 2008-04-09
CA2240334A1 (en) 1997-06-19
WO1997021821A1 (en) 1997-06-19
DE69613856T2 (de) 2002-04-04
US5744312A (en) 1998-04-28
CA2240334C (en) 2008-07-22
ATE203056T1 (de) 2001-07-15
JP2000501616A (ja) 2000-02-15
EP0866868B1 (en) 2001-07-11

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