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ES2089970B1 - ISOLATION METHOD OF LACTOFERRIN AND OTHER DAIRY PROTEINS. - Google Patents

ISOLATION METHOD OF LACTOFERRIN AND OTHER DAIRY PROTEINS.

Info

Publication number
ES2089970B1
ES2089970B1 ES9400477A ES9400477A ES2089970B1 ES 2089970 B1 ES2089970 B1 ES 2089970B1 ES 9400477 A ES9400477 A ES 9400477A ES 9400477 A ES9400477 A ES 9400477A ES 2089970 B1 ES2089970 B1 ES 2089970B1
Authority
ES
Spain
Prior art keywords
lactoferrin
isolation
cellulose
isolation method
dairy proteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES9400477A
Other languages
Spanish (es)
Other versions
ES2089970A1 (en
Inventor
Rebollar Miguel Calvo
Dalmau Jose Manuel Ena
Paniagua Lourdes Sanchez
Cabrejas Dolores Perez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universidad de Zaragoza
Original Assignee
Universidad de Zaragoza
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universidad de Zaragoza filed Critical Universidad de Zaragoza
Priority to ES9400477A priority Critical patent/ES2089970B1/en
Publication of ES2089970A1 publication Critical patent/ES2089970A1/en
Application granted granted Critical
Publication of ES2089970B1 publication Critical patent/ES2089970B1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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  • Peptides Or Proteins (AREA)

Abstract

EL OBJETO DE LA INVENCION ES UN METODO DE AISLAMIENTO DE LA LACTOFERRINA BASADO EN SU ABSORCION ESPECIFICA MEDIANTE UN LIGANDO INSOLUBILIZADO EN PLACAS DE CARTON DE CELULOSA FILTRANTE. EL AISLAMIENTO DE LACTOFERRINA SE HACE A PARTIR DE LACTOSUERO PROCEDENTE DE QUESERIA. ESTE SE HACE PASAR EN FLUJO CONTINUO POR UN DISPOSITIVO EN EL QUE SE ENCUENTRAN LAS PLACAS DE CELULOSA MODIFICADAS CON AZUL REACTIVO2, QUE RETIENEN FUNDAMENTALMENTE LA LACTOFERRINA. UNA VEZ LAVADA LA CELULOSA PARA ELIMINAR LAS PROTEINAS NO RETENIDAS, SE ELUYEN LAS RETENIDAS CON UNA SOLUCION SALINA. FINALMENTE, LA LACTOFERRINA OBTENIDA SE DIALIZA PARA ELIMINAR PARTE DE LA SAL Y SE LIOFILIZA, TRAS LO CUAL PUEDE SER UTILIZADA PARA SU ADICION A PRODUCTOS PARA USO ANIMAL O HUMANO.THE OBJECT OF THE INVENTION IS A METHOD OF ISOLATION OF LACTOFERRIN BASED ON ITS SPECIFIC ABSORPTION THROUGH A LIGAND INSOLUBILIZED IN FILTER CELLULOSE CARDBOARD PLATES. THE ISOLATION OF LACTOFERRIN IS MADE FROM LACTOSUERO FROM CHEESE. THIS IS PASSED IN A CONTINUOUS FLOW THROUGH A DEVICE IN WHICH THE REAGENT BLUE MODIFIED CELLULOSE PLATES ARE FOUND, WHICH FUNDAMENTALLY RETAIN THE LACTOFERRIN. ONCE THE CELLULOSE IS WASHED TO ELIMINATE NON-RETAINED PROTEINS, RETAINED PROTEINS ARE ELUTIOUS WITH A SALT SOLUTION. FINALLY, THE LACTOFERRIN OBTAINED IS DIALYZED TO ELIMINATE PART OF THE SALT AND IS LYOPHILIZED, AFTER WHICH IT CAN BE USED FOR ITS ADDITION TO PRODUCTS FOR ANIMAL OR HUMAN USE.

ES9400477A 1994-03-08 1994-03-08 ISOLATION METHOD OF LACTOFERRIN AND OTHER DAIRY PROTEINS. Expired - Fee Related ES2089970B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9400477A ES2089970B1 (en) 1994-03-08 1994-03-08 ISOLATION METHOD OF LACTOFERRIN AND OTHER DAIRY PROTEINS.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9400477A ES2089970B1 (en) 1994-03-08 1994-03-08 ISOLATION METHOD OF LACTOFERRIN AND OTHER DAIRY PROTEINS.

Publications (2)

Publication Number Publication Date
ES2089970A1 ES2089970A1 (en) 1996-10-01
ES2089970B1 true ES2089970B1 (en) 1997-05-01

Family

ID=8285479

Family Applications (1)

Application Number Title Priority Date Filing Date
ES9400477A Expired - Fee Related ES2089970B1 (en) 1994-03-08 1994-03-08 ISOLATION METHOD OF LACTOFERRIN AND OTHER DAIRY PROTEINS.

Country Status (1)

Country Link
ES (1) ES2089970B1 (en)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2613368B1 (en) * 1987-03-10 1989-06-23 Entremont Sa PROCESS FOR EXTRACTING WHEY PROTEINS BY ADSORPTION AND ELUTION
ATE129125T1 (en) * 1992-01-15 1995-11-15 Campina Melkunie Bv METHOD FOR ISOLATION OF LACTOFERRIN AND LACTOPEROXIDASE FROM MILK AND MILK PRODUCTS.

Also Published As

Publication number Publication date
ES2089970A1 (en) 1996-10-01

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Date Code Title Description
FD2A Announcement of lapse in spain

Effective date: 20141120