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ES2067480T3 - Metodo de inmunoensayo para la deteccion de antigenos. - Google Patents

Metodo de inmunoensayo para la deteccion de antigenos.

Info

Publication number
ES2067480T3
ES2067480T3 ES88312056T ES88312056T ES2067480T3 ES 2067480 T3 ES2067480 T3 ES 2067480T3 ES 88312056 T ES88312056 T ES 88312056T ES 88312056 T ES88312056 T ES 88312056T ES 2067480 T3 ES2067480 T3 ES 2067480T3
Authority
ES
Spain
Prior art keywords
antigen
antibody
assay method
methods
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES88312056T
Other languages
English (en)
Inventor
Gary Lee Milburn
Thomas Michael Houts
Judith Rabbie
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Syntex USA LLC
Original Assignee
Syntex USA LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syntex USA LLC filed Critical Syntex USA LLC
Application granted granted Critical
Publication of ES2067480T3 publication Critical patent/ES2067480T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/571—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses for venereal disease, e.g. syphilis, gonorrhoea
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/871—Neisseria
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/824—Immunological separation techniques
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/828—Protein A

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Food Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

SE DESCRIBEN METODOS PARA DETECTAR UN ANTIGENO EN UNA MUESTRA BIOLOGICA.. LOS METODOS INCLUYEN PROPORCIOANR UN SOPORTE SOLIDO EN COMBINACION, QUE ESTA PRACTICAMENTE LIBRE DE PROTEINAS LIGANTES ESPECIFICAS, Y UN MEDIO QUE COMPRENDE UN ANTIGENO DE UNA MUESTRA Y UN ANTICUERPO PARA EL ANTIGENO. LA COMBINACION SE INCUBA EN CONDICIONES SUFICIENTES PARA QUE EL ANTICUERPO CUANDO ESTA ENLAZADO AL ANTIGENO, ENLACE AL SOPORTE. LA PRESENCIA O CANTIDAD DE ANTICUERPO SOBRE EL SOPORTE O EN EL MEDIO SE DETERMINA Y RELACIONA CON LA PRESENCIA DE ANTIGENO EN LA MUESTRA. LOS METODOS TIENEN ESPECIAL APLICACION A LA DETECCION DE BACTERIAS GAM-NEGATIVAS.
ES88312056T 1987-12-21 1988-12-20 Metodo de inmunoensayo para la deteccion de antigenos. Expired - Lifetime ES2067480T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/135,869 US4959303A (en) 1987-12-21 1987-12-21 Assay for antigens by binding immune complexes to solid supports free of protein and non-ionic binders

Publications (1)

Publication Number Publication Date
ES2067480T3 true ES2067480T3 (es) 1995-04-01

Family

ID=22470097

Family Applications (1)

Application Number Title Priority Date Filing Date
ES88312056T Expired - Lifetime ES2067480T3 (es) 1987-12-21 1988-12-20 Metodo de inmunoensayo para la deteccion de antigenos.

Country Status (7)

Country Link
US (1) US4959303A (es)
EP (1) EP0325045B1 (es)
JP (1) JP2892662B2 (es)
AT (1) ATE118275T1 (es)
CA (1) CA1336675C (es)
DE (1) DE3852996T2 (es)
ES (1) ES2067480T3 (es)

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US5482830A (en) * 1986-02-25 1996-01-09 Biostar, Inc. Devices and methods for detection of an analyte based upon light interference
US5866322A (en) * 1988-01-29 1999-02-02 Abbott Laboratories Method for performing Rubella assay
US5459078A (en) * 1988-01-29 1995-10-17 Abbott Laboratories Methods and reagents for performing ion-capture digoxin assays
US5459080A (en) * 1988-01-29 1995-10-17 Abbott Laboratories Ion-capture assays using a specific binding member conjugated to carboxymethylamylose
US5116726A (en) * 1988-07-25 1992-05-26 Syntex (U.S.A.) Inc. Methods for removal of detergents from analytes
US5225330A (en) * 1988-08-01 1993-07-06 The United States Of America As Represented By The Department Of Health And Human Services Diagnostic kit and diagnostic method utilizing carbohydrate receptors
US5075220A (en) * 1988-10-07 1991-12-24 Eastman Kodak Company Determination of a chlamydial or gonococcal antigen using a positively-charged ionically binding support
DE68922888T2 (de) * 1988-10-07 1995-11-30 Eastman Kodak Co Verwendung eines kationischen Oberflächenaktivmittels zur Extraktion des Hauptprotein-Antigens der äusseren Membran von Chlamydia.
US5132205A (en) * 1988-10-07 1992-07-21 Eastman Kodak Company High ph extraction composition and its use to determine a chlamydial, gonococcal or herpes antigen
US5032504A (en) * 1988-10-07 1991-07-16 Eastman Kodak Company Diagnostic test kit and method for determination of chlamydial or gonococcal antigens using a microporous membrane
DE69010545T2 (de) * 1989-04-14 1994-11-10 Unilever Nv Extraktionsverfahren.
US5639671A (en) * 1989-09-18 1997-06-17 Biostar, Inc. Methods for optimizing of an optical assay device
US5541057A (en) * 1989-09-18 1996-07-30 Biostar, Inc. Methods for detection of an analyte
US5187066A (en) * 1990-02-14 1993-02-16 Syntex (U.S.A.) Inc. Methods for detecting amphiphilic antigens
CA2048302A1 (en) * 1990-08-15 1992-02-16 Victoria P. Meucci Solubilization reagent for biological test samples
US5550063A (en) * 1991-02-11 1996-08-27 Biostar, Inc. Methods for production of an optical assay device
US5955377A (en) * 1991-02-11 1999-09-21 Biostar, Inc. Methods and kits for the amplification of thin film based assays
US5418136A (en) * 1991-10-01 1995-05-23 Biostar, Inc. Devices for detection of an analyte based upon light interference
US5334503A (en) * 1991-10-08 1994-08-02 Eastman Kodak Company Test kit and method for the detection of microorganisms associated with periodontal diseases using surfactant mixture as extraction composition
US5449619A (en) * 1992-04-16 1995-09-12 Sybron Chemical Holdings, Inc. Drain opener formulation
US5494829A (en) * 1992-07-31 1996-02-27 Biostar, Inc. Devices and methods for detection of an analyte based upon light interference
US5552272A (en) * 1993-06-10 1996-09-03 Biostar, Inc. Detection of an analyte by fluorescence using a thin film optical device
US5773234A (en) * 1995-08-07 1998-06-30 Quidel Corporation Method and device for chlamydia detection
AU1532797A (en) * 1996-01-16 1997-08-11 Sybron Chemical Holdings, Inc. Cleaner and sanitizer formulation
CA2231569A1 (en) * 1996-07-18 1998-01-29 Behringwerke Aktiengesellschaft Reagents for assays for mycophenolic acid
WO1998003877A1 (en) 1996-07-18 1998-01-29 Behringwerke Aktiengesellschaft Reagents for assays for ligands
US5871905A (en) * 1996-09-04 1999-02-16 Epitope, Inc. Reduction of false positives in oral-fluid based immunoassays
US5856194A (en) 1996-09-19 1999-01-05 Abbott Laboratories Method for determination of item of interest in a sample
US5795784A (en) 1996-09-19 1998-08-18 Abbott Laboratories Method of performing a process for determining an item of interest in a sample
US5929049A (en) * 1997-08-08 1999-07-27 Dade Behring Marburg Gmbh Polysaccharide conjugates of biomolecules
DE19808226C1 (de) * 1998-02-27 2000-03-02 Bruker Analytik Gmbh Anordnung und Verfahren zur Untersuchung von hydrophilen Makromolekülen in wässriger Lösung
US6153442A (en) * 1998-05-20 2000-11-28 Dade Behring Inc. Reagents and methods for specific binding assays
US6303325B1 (en) 1998-05-29 2001-10-16 Dade Behring Inc. Method for detecting analytes
EP1122542A1 (en) 2000-02-01 2001-08-08 Anda Biologicals S.A. Method for the rapid detection of whole microorganisms on retaining membranes by use of chaotropic agents
US9575070B2 (en) * 2001-12-04 2017-02-21 Wayne State University Neoepitope detection of disease using protein arrays
AU2003275065A1 (en) * 2002-09-20 2004-04-08 Wayne State University Molecular targets of cancer and aging
JP2005291783A (ja) * 2004-03-31 2005-10-20 Denka Seiken Co Ltd 免疫測定に供する検体浮遊液調製用媒体組成物及びそれを用いる免疫測定方法
JP2006084351A (ja) * 2004-09-16 2006-03-30 Denka Seiken Co Ltd 検体浮遊液組成物、キット及び検査方法
AU2017240233B2 (en) * 2016-03-31 2022-07-14 University Of Southern California A highly sensitive and specific luciferase based reporter assay for antigen detection

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US4283383A (en) * 1974-05-20 1981-08-11 Technicon Instruments Corporation Analysis of biological fluids
US4145406A (en) * 1977-04-11 1979-03-20 Miles Laboratories, Inc. Specific binding - adsorbent assay method and test means
US4254082A (en) * 1978-06-12 1981-03-03 Miles Laboratories, Inc. Specific binding-adsorbent assay test means
US4255412A (en) * 1978-12-19 1981-03-10 Beckman Instruments, Inc. T3 Uptake test employing a precipitated nylon separating agent
US4427782A (en) * 1981-03-03 1984-01-24 Caldwell Harlan D Isolation of principal outer membrane protein and antigen of Chlamydia trachomatis
US4450231A (en) * 1982-03-31 1984-05-22 Biostar Medical Products, Inc. Immunoassay for determination of immune complexes with polymer-coated plastic base
US4497899A (en) * 1982-04-12 1985-02-05 Abbott Laboratories Immunoassay for Chlamydia trachomatis antigens
US4503143A (en) * 1982-08-20 1985-03-05 Btc Diagnostics Limited Partnership Enzyme immunoassay with two-part solution of tetramethylbenzidine as chromogen
GB8305197D0 (en) * 1983-02-24 1983-03-30 Amersham Int Plc Assay methods
CA1220147A (en) * 1984-06-14 1987-04-07 Terry W. Pearson Detection of gonococcal infections using monoclonal antibodies
GB8427006D0 (en) * 1984-10-25 1984-11-28 Iq Bio Ltd Determination of chlamydia trachomatis
JPS61110055A (ja) * 1984-11-02 1986-05-28 Daicel Chem Ind Ltd 分析方法
US4757024A (en) * 1985-05-31 1988-07-12 Biostar Medical Products, Inc. Immune complex detection method and article using immunologically non-specific immunoglobulins
TW203120B (es) * 1985-10-04 1993-04-01 Abbott Lab
DE3787078T2 (de) * 1986-11-24 1993-12-09 Abbott Lab Proberichtwirkung für analytisches Festphasengerät.

Also Published As

Publication number Publication date
JPH02138869A (ja) 1990-05-28
US4959303A (en) 1990-09-25
EP0325045B1 (en) 1995-02-08
EP0325045A3 (en) 1990-05-30
EP0325045A2 (en) 1989-07-26
CA1336675C (en) 1995-08-15
DE3852996T2 (de) 1995-06-01
DE3852996D1 (de) 1995-03-23
JP2892662B2 (ja) 1999-05-17
ATE118275T1 (de) 1995-02-15

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