EP4107276A1 - Method for the incorporation of formaldehyde into biomass - Google Patents

Abstract

Described is a method for the incorporation of formaldehyde into biomass comprising the following enzymatically catalyzed steps (1) condensation of pyruvate with formaldehyde into 4-hydroxy-2-oxobutanoic acid (HOB); (2) amination of the thus produced 4-hydroxy-2-oxobutanoic acid (HOB) to produce homoserine; (3) conversion of thus produced homoserine to threonine; (4) conversion of the thus produced threonine into glycine and acetaldehyde or acetyl-CoA; (5) condensation of the thus produced glycine with formaldehyde to produce serine; and (6) conversion of the thus produced serine to produce pyruvate, wherein said pyruvate can then be used as a substrate in step (1).

Description

Method for the incorporation of formaldehyde into biomass

The present invention relates to a method or enzymatic pathway comprising the following enzymatically catalyzed steps

(1) condensation of pyruvate with formaldehyde into 4-hydroxy-2-oxobutanoic acid (HOB);

(2) amination of the thus produced 4-hydroxy-2-oxobutanoic acid (HOB) to produce homoserine;

(3) conversion of thus produced homoserine to threonine;

(4) conversion of the thus produced threonine into glycine and acetaldehyde or acetyl-CoA;

(5) condensation of the thus produced glycine with formaldehyde to produce serine; and

(6) conversion of the thus produced serine to produce pyruvate, wherein said pyruvate can then be used as a substrate in step (1).

This method/pathway allows for the efficient incorporation of formaldehyde into carbon compounds and thus of the conversion of formaldehyde into biomass. The present invention also relates to recombinant microorganisms which express the enzymes for catalyzing the corresponding enzymatic reactions.

Microbial production of commodity chemicals is limited by feedstock availability and cost. Sugars and starches, despite being commonly used, are not ideal microbial feedstocks as their biotechnological utilization directly competes with human consumption, thus eroding food security (Walker, J. Appl. Phycol. 21 (2009), 509- 517). Furthermore, the expansion of agricultural cultivation comes at the expense of shrinking natural habitats, hence threatening biodiversity (Scholes et al., in Intergovernmental Science- Policy Platform on Biodiversity and Ecosystem Services (Bonn, Germany; 2018)). The use of lignocellulosic biomass, while avoiding some of these problems, presents other challenges, including heterogenic composition, difficult processing, and deleterious waste products (Sanderson, Nature 474 (2011), 12-14). One carbon compounds provide a favorable alternative as they can be produced at high levels without burdening agricultural production and they represent homogenous, easy-to-handle microbial feedstocks (Takors et al., Microb. Biotechnol. 11 (2018), 606-625; Yishai et al., Curr. Opin. Chem. Biol. 35 (2016), 1-9; Schrader et al. , Trends Biotechnol. 27 (2009), 107-115). Methanol is especially interesting as it is completely water miscible, avoiding mass transfer barriers that constrain the use of gaseous one carbon compounds (e.g., carbon monoxide and methane). Methanol can be produced at low-cost from natural gas (Zakaria and Kamarudin, Renew. Sust. Energ. Rev. 65 (2016), 250-261), or it can be produced sustainably and efficiently from CO2 and electrochemically derived hydrogen (Szima and Cormos, J. CO2 Util. 24 (2018), 555-563). Methanol bio-assimilation mostly involves its oxidation to formaldehyde, followed by fomaldehyde incorporation into biomass via a dedicated cycle.

There has been much recent progress in the metabolic engineering of microorganisms that naturally grow on methanol, e.g., Methylobacterium extorquens (Schada von Borzyskowski et al., ACS Synthetic Biology 4 (2015), 430-443; Marx and Lidstrom, Microbiology 150 (2004), 9-19). Also multiple recent efforts have sought to engineer biotechnological hosts for growth on methanol via one of the naturally occurring methanol assimilation pathways (Whitaker, Curr. Opin. Biotechnol. 33 (2015), 165-175; Zhang et al., RSC Advances 7, (2017) 4083-4091): the ribulose monophosphate (RuMP) cycle (Chen et al., Metab. Eng. 49, (2018) 257-266; Meyer et al., Nature Communications 9, (2018) 1508; He et al., ACS Synthetic Biology 7, (2018) 1601-1611), the dihydroxyacetone (DHA) cycle (Dai et al., Bioresour. Technol. 245 (2017), 1407-1412) and the serine cycle (Yu and Liao, Nature Communications 9 (2018), 3992). However, these natural pathways might not represent optimal solutions. Better pathways, more efficient in the use of cellular resources and/or more metabolically compatible with the host microorganism, could be designed and implemented. For example, a recent study designed and partially implemented a modified serine cycle in E. coli, where some of the natural reactions were replaced with others, better fitting the endogenous metabolism of the host (Yu and Liao, Nature Communications 9 (2018), 3992). However, the serine cycle and its modified variants are ATP-inefficient, which results in low biomass and product yields (Claassens et al., Nature Catalysis 2 (2019), 437). In other studies, a new-to-nature formolase reaction was demonstrated, where two or three formaldehyde molecules are condensed to generate glycolaldehyde or DHA, respectively (Lu et al., Nature Communications 10 (2019), 1378; Wang et al., Bioresour. Bioprocess. 4 (2017), 41). However, the condensation rate and affinity for formaldehyde are too low to be physiologically relevant.

Thus, there is a need to develop means and methods which allow for the efficient assimilation and incorporation of formaldehyde (which may be produced from methanol) into biomass thereby facilitating the use of, e.g., methanol as a feedstock for bioproduction. The present invention meets this demand by providing a method or enzymatic pathway comprising the following enzymatically catalyzed steps

(1) condensation of pyruvate with formaldehyde into 4-hydroxy-2-oxobutanoic acid (HOB);

(2) amination of the thus produced 4-hydroxy-2-oxobutanoic acid (HOB) to produce homoserine;

(3) conversion of thus produced homoserine to threonine;

(4) conversion of the thus produced threonine into glycine and acetaldehyde or acetyl-CoA;

(5) condensation of the thus produced glycine with formaldehyde to produce serine; and

(6) conversion of the thus produced serine to produce pyruvate, wherein said pyruvate can then be used as a substrate in step (1).

This method/pathway allows for the efficient incorporation of formaldehyde into carbon compounds and thus its conversion into biomass.

The above specified sequence of enzymatic steps represents a cycle (in the following also referred to as the “homoserine cycle”), a non-limiting example of which is shown in Figure 1. The inventors in their effort to provide more efficient means for formaldehyde assimilation started out from the above-mentioned serine cycle and provided an improved variant thereof which allows for a more efficient incorporation of formaldehyde. The provided pathway also has the advantage that it can easily be implemented in microorganisms which are frequently used for biotechnological production methods, such as E. coli.

In the homoserine cycle, pyruvate is condensed with formaldehyde to generate the non-natural metabolite 4-hydroxy-2-oxobutanoate (HOB) (Bouzon et al. , ACS Synthetic Biology 6 (2017), 1520-1533), which is subsequently aminated to homoserine. The first of these reactions - (item (1) in Fig. 1) - was found to be promiscuously catalyzed by E. coli 2-keto-3-deoxy-L-rhamnonate aldolase (RhmA) (Hernandez et al., ACS Catal. 7 (2017), 1707-1711). The latter reaction - HOB amination (item (2) in Fig. 1) - is supported by numerous aminotransferases (Hernandez et al., ACS Catal. 7 (2017), 1707-1711; Walther et al., Metab. Eng. 45 (2018), 237-245; Zhong et al., ACS Synthetic Biology 8 (2019), 587-595) as well as amino acid dehydrogenases such as (engineered) glutamate dehydrogenase (Chen et al., Biotechnol. J. 10 (2015), 284-289). While the literature mostly reports the reaction in the reverse direction, it can also be used in the direction of HOB amination since it is reversible. This route effectively replaces a carboxylation reaction (by phosphoenolpyruvate carboxylase) with a formaldehyde assimilation reaction that provides an alternative way to generate a C₄ intermediate. Homoserine is then converted into threonine, e.g. by the action of homoserine kinase (ThrB, item (3) in Fig. 1) and threonine synthase (ThrC, item (4) in Fig. 1). Threonine is cleaved to produce glycine and acetaldehyde. This can, e.g., be achieved by making use of a threonine aldolase (item (5) in Fig. 1, for example by the same threonine aldolase (LtaE) that catalyzes the SAL reaction (item (6) in Figure 1) to regenerate glycine and produce acetaldehyde. The produced acetaldehyde can, e.g., be further oxidized to acetyl-CoA and assimilated to central metabolism.

In the homoserine cycle, the glycine produced by the cleavage of threonine is directly condensed with formaldehyde to generate serine. This reaction (item (6) in Fig. 1) (herein also referred to as the serine aldolase (SAL) reaction) was previously found to be promiscuously catalyzed {in vitro) by a threonine aldolase (LtaE) (Contestabile et al. , Eur. J. Biochem. 268 (2001), 6508-6525). The SAL reaction bypasses the known very long, multi-cofactor-dependent, and ATP-inefficient route for formaldehyde assimilation to 5,10-methylene-tetrahydrofolate (CH₂-THF) (Crowther et al., J. Bacteriol. 190 (2008), 5057-5062). As within the previously proposed modified serine cycles (Yu and Liao, Nature Communications 9 (2018), 3992; Bar- Even, Biochemistry 55 (2016), 3851-3863), serine is then deaminated to pyruvate by serine deaminase (item (7) in Fig. 1), bypassing a longer route via glycerate, which further involves the highly toxic intermediate hydroxy pyruvate (Kim and Copley, Proc. Natl. Acad. Sci. USA 109 (2012), E2856-2864). The resulting pyruvate can then be used in step (1 ) of the method thereby closing the cycle.

It is shown that this newly proposed synthetic route can outperform the serine cycle and its known variants with respect to biomass yield, thermodynamic favorability, and implementation into host endogenous metabolism.

The inventors could also demonstrate the in vivo activity of the cycle and, in particular, the functionality of those reactions of the pathway which rely on non natural reactions. More precisely, the inventors demonstrated by way of experiments the in vivo activity of different pathway segments including all the required activies, in particular also the promiscuous enzyme activities which can be provided by a host cell itself without the need to have it genetically modified to express these enzyme activities. The cycle can efficiently provide building blocks which are essential for the production of biomass. The data provided in the present application confirm that the proposed pathway will allow the efficient assilimation of methanol (via formaldehyde) and, thus, the implementation of a highly efficient conversion of this one carbon feedstock into commodity chemicals.

The method according to the present invention differs from the known serine cycle, e.g., in that it replaces C0₂ fixation with formaldehyde assimilation. For this purpose, it relies on two formaldehyde-condensing reactions which are catalyzed (promiscuously) by aldolases.

As indicated above, the method or pathway according to the present invention allows for the incorporation of formaldehyde. The term “incorporation of formaldehyde” means that formaldehyde is assimilated into carbon compounds that are part of microbial central metabolism and can, thus, be converted into biomass and/or desired compounds. Thus, the method/pathway can be used for the assimilation of formaldehyde into carbon compounds and its incorporation into biomass.

Step 1 of the method: condensation of pyruvate with formaldehyde so as to produce 4-hvdroxy-2-oxobutanoic acid (HOB)

In a first step of the method/pathway, pyruvate is condensed in an enzymatically catalyzed reaction with formaldehyde so as to produce 4-hydroxy-2-oxobutanoic acid (HOB).

In a preferred embodiment, this condensation is achieved by making use of an aldolase and more preferably of an aldolase which is classified in EC 4.1 2._. Examples of aldolases classified in EC 4.1 2._ which may be particularly useful in the context of the method/pathway of the present invention are aldolases selected from the following:

(i) aldolases classified in EC 4.1.2.53 (2-keto-3-deoxy-L-rhamnonate aldolase);

(ii) aldolases classified in EC 4.1.2.51 (2-dehydro-3-deoxy-D-gluconate aldolase);

(iii) aldolases classified in EC 4.1.2.28 (2-dehydro-3-deoxy-D-pentonate aldolase);

(iv) aldolases classified in EC 4.1.2.20 (2-dehydro-3-deoxyglucarate aldolase).

In one preferred embodiment, the aldolase is an aldolase which belongs to the HpcH aldolase family. Aldolases classified as belonging to the HpcH aldolase family are characterized in that they domain referred to as “HpcH” (PF03328; http://pfam.xfam.ora/familv/PF03328). Moreover, such aldolases are preferably characterized in using a catalytic mechanism which involves a divalent metal cation for donor binding and enolization. Examples for such aldolases are the aldolases classified as EC 4.1.2.53 or classified as EC 4.1.2.20.

In another embodiment, the aldolase is an aldolase which belongs to the DHDPS aldolase family. Aldolases classified as belonging to the DHDPS aldolase family are characterized in that they domain referred to as “DHDPS” (PF00701, http://pfam.xfam.org/familv/PF00701). Moreover, such aldolases are preferably characterized in using a catalytic mechanism which involves a catalytic lysine residue to form a Schiff base with the donor substrate. Examples for such aldolases are the aldolases classified as EC 4.1.2.51 or classified as EC 4.1.2.28.

The condensation of pyruvate with formaldehyde into 4-hydroxy-2-oxobutanoic acid (HOB) is a reaction which is not known to occur naturally. However, there was a report in the literature (Hernandez et al. , ACS Catal. 7 (2017), 1707-1711) that an aldolase from E. coli, i.e. the RhmA/Yfall (EC 4.1.2.53) protein, can promiscuously catalyze this conversion. Moreover, the inventors could show that this conversion can actually be catalyzed by various aldolases in a promiscuous manner, i.e. these enzymes can use pyruvate and formaldehyde as substrates although their natural substrates are different from these compounds. Moreover, as is evident from the appended Examples, the inventors could also show that corresponding aldolases are capable of catalyzing the corresponding reaction in vivo and to an extent which leads to an efficient condensation of pyruvate and formaldehyde into 4-hydroxy-2- oxobutanoic acid (HOB).

Aldolases classified in EC 4.1.2.53 (2-keto-3-deoxy-L-rhamnonate aldolase) naturally catalyze the reaction

2-dehydro-3-deoxy-L-rhamnonate pyruvate + (S)-lactaldehyde

, i.e. the reversible retro-aldo cleavage of 2-dehydro-3-deoxy-L-rhamnonate to pyruvate and (S)-lactaldehyde (Rakus et al., Biochemistry 47 (2008), 9944-9954; Read et al., Biochemistry 47 (2008), 9955-9965).

This enzyme has been identified in several organisms, e.g. in Azetobacter vinelandii, in Scheffersomyces stipitis and Schwanniomyces polymorphus as well as in E. coli (Uniprot accession numbers: P76469 and D3QKU2). In principle any 2-keto-3-deoxy- L-rhamnonate aldolase (EC 4.1.2.53) can be employed in the method according to the present invention as long as it can catalyze the condensation of pyruvate with formaldehyde into 4-hydroxy-2-oxobutanoic acid (HOB). In a preferred embodiment an enzyme from E. coli is used, in particular an enzyme encoded by the rhmA gene of E. coli or by the yfaU gene of E. coli (Hernandez et al., ACS Catal. 7 (2017), 1707- 1711).

In a preferred embodiment such an enzyme has an amino acid sequence as shown in SEQ ID NO: 1 or shows an amino acid sequence which is at least x% homologous to SEQ ID NO: 1 and has the activity of a 2-keto-3-deoxy-L-rhamnonate aldolase (EC 4.1.2.53) with x being an integer between 60 and 100, preferably 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98 or 99 wherein such an enzyme is capable of catalyzing the condensation of pyruvate with formaldehyde into 4-hydroxy-2- oxobutanoic acid (HOB).

Preferably, the degree of identity is determined by comparing the respective sequence with the amino acid sequence of SEQ ID NO: 1.

As regards the determination of sequence identity as described in the present application, generally the following should apply: When the sequences which are compared do not have the same length, the degree of identity either refers to the percentage of amino acid residues in the shorter sequence which are identical to amino acid residues in the longer sequence or to the percentage of amino acid residues in the longer sequence which are identical to amino acid residues in the shorter sequence. Preferably, it refers to the percentage of amino acid residues in the shorter sequence which are identical to amino acid residues in the longer sequence. The degree of sequence identity can be determined by performing pairwise alignment using preferably algorithms and software well known in the art, such as Needleman- Wunsch algorithm with the EMBOSS NEEDLE software.

When applying this methodology to determine whether a particular sequence is, for instance, at least 60% identical to a reference sequence default settings of the EMBOSS NEEDLE software may be used, which are defined as follows :

- Matrix : BLOSUM62

- Gap open: 10

- Gap extend : 0.5

- No end gap penalty.

Preferably, the degree of identity is calculated over the complete length of the aligned sequence.

Aldolases classified in EC 4.1.2.51 (2-dehydro-3-deoxy-D-gluconate aldolase) naturally catalyze the reaction

2-dehydro-3-deoxy-D-gluconate D-glyceraldehyde + pyruvate

, i.e. the formation of 2-keto-3-deoxy-gluconate (KDG) from pyruvate and glyceraldehyde (Bhaskar et al. , Proteins 79 (2011 ), 1132-1142), or the reaction (Liu et al., Appl. Microbiol. Biotechnol 97 (2013), 3409-3417):

2-dehydroxy-3-deoxy-D-arabinoate glycolaldehyde + pyruvate The enzyme has, e.g., been idendified in Picrophilus torridus and in E. coli. In principle any 2-dehydro-3-deoxy-D-gluconate aldolase (EC 4.1.2.51) can be employed in the method according to the present invention as long as it can catalyze the condensation of pyruvate with formaldehyde into 4-hydroxy-2-oxobutanoic acid (HOB). In a preferred embodiment an enzyme from E. coli is used, in particular an enzyme encoded by the yagE gene of E. coli (Uniprot accession number P75682).

In a preferred embodiment such an enzyme has an amino acid sequence as shown in SEQ ID NO: 2 or shows an amino acid sequence which is at least x% homologous to SEQ ID NO: 2 and has the activity of a 2-dehydro-3-deoxy-D-gluconate aldolase (EC 4.1.2.51) with x being an integer between 60 and 100, preferably 65, 70, 75, 80, 85, 90, 91 , 92, 93, 94, 95, 96, 97, 98 or 99 wherein such an enzyme is capable of catalyzing the condensation of pyruvate with formaldehyde into 4-hydroxy-2- oxobutanoic acid (HOB).

Preferably, the degree of identity is determined by comparing the respective sequence with the amino acid sequence of SEQ ID NO: 2. As regards the determination of the degree of sequence identity the same applies as had been set forth above.

Aldolases classified in EC 4.1.2.28 (2-dehydro-3-deoxy-D-pentonate aldolase) naturally catalyze the reaction (Liu et al. , Appl. Microbiol. Biotechnol 97 (2013), 3409- 3417):

2-dehydro-3-deoxy-D-arabinonate glycolaldehyde + pyruvate

The enzyme has, e.g., been idendified in Pseudomonas sp. (Dahms, A.S.; Biochem. Biophys. Res. Commun. 60, 1433-1439 (1974)) and in E. coli (Uniprot accession number: P39359). In principle any 2-dehydro-3-deoxy-D-pentonate aldolase (EC 4.1.2.28) can be employed in the method according to the present invention as long as it can catalyze the condensation of pyruvate with formaldehyde into 4-hydroxy-2- oxobutanoic acid (HOB). In a preferred embodiment an enzyme from E. coli is used, in particular an enzyme encoded by the yjhH gene of E. coli (Liu et al., loc cit. ; Uniprot accession number: P39359).

In a preferred embodiment such an enzyme has an amino acid sequence as shown in SEQ ID NO: 3 or shows an amino acid sequence which is at least x% homologous to SEQ ID NO: 3 and has the activity of a 2-dehydro-3-deoxy-D-pentonate aldolase (EC 4.1.2.28) with x being an integer between 60 and 100, preferably 65, 70, 75, 80, 85, 90, 91 , 92, 93, 94, 95, 96, 97, 98 or 99 wherein such an enzyme is capable of catalyzing the condensation of pyruvate with formaldehyde into 4-hydroxy-2- oxobutanoic acid (HOB). Preferably, the degree of identity is determined by comparing the respective sequence with the amino acid sequence of SEQ ID NO: 3. As regards the determination of the degree of sequence identity the same applies as had been set forth above.

Aldolases classified in EC 4.1.2.20 (2-dehydro-3-deoxyglucarate aldolase) naturally catalyze the reaction

2-dehydro-3-deoxyglucarate ^=^2-hydroxy-3-oxopropanoate + pyruvate as, e.g., described in Hubbard et al., Biochemistry 37 (1998), 14369-14375).

This enzyme has been identified in several organisms, e.g. in Klebsiella aerogenes, Leptospira interrogans, Picrophilus torridus and in E. coli (Uniprot accession number: P23522). In principle any 2-dehydro-3-deoxyglucarate aldolase (EC 4.1.2.20) can be employed in the method according to the present invention as long as it can catalyze the condensation of pyruvate with formaldehyde into 4-hydroxy-2-oxobutanoic acid (HOB). In a preferred embodiment an enzyme from E. coli is used, in particular an enzyme encoded by the garL gene of E. coli (Uniprot accession number: P23522).

In a preferred embodiment such an enzyme has an amino acid sequence as shown in SEQ ID NO: 4 or shows an amino acid sequence which is at least x% homologous to SEQ ID NO: 4 and has the activity of a 2-dehydro-3-deoxyglucarate aldolase (EC 4.1.2.20) with x being an integer between 60 and 100, preferably 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98 or 99 wherein such an enzyme is capable of catalyzing the condensation of pyruvate with formaldehyde into 4-hydroxy-2- oxobutanoic acid (HOB).

Preferably, the degree of identity is determined by comparing the respective sequence with the amino acid sequence of SEQ ID NO: 4. As regards the determination of the degree of sequence identity the same applies as had been set forth above.

Step 2 of the method: conversion of 4-hvdroxy-2-oxobutanoic acid (HOB) into homoserine

According to the method/pathway of the present invention, the 4-hydroxy-2- oxobutanoic acid (HOB) produced in step (1) is further enzymatically converted in step (2) into homoserine by an amination reaction.

It is known that in nature three different enzyme families can convert an amino acid to the corresponding a-keto acid (or the reversible reaction), namely: (1) amino acid transaminase (aminotransferases), (2) amino acid dehydrogenase; and (3) amino acid deaminase (oxidase). In the method of the present invention, in principle enzymes from any one of these groups can be employed for catalyzing the conversion of HOB into homoserine. Since basically all organisms, including all microorganisms, express enzymes of the above-mentioned three groups, the method of the present invention, when implemented in vivo in an organisms can rely on endogenously occurring enzyme activities of any of the above-mentioned three groups for achieving the conversion of HOB into homoserine.

The amination can preferably be achieved by an aminotransferase enzyme or by an amino acid dehydrogenase enzyme. In a preferred embodiment, the amination is achieved by making use of an aminotransferase classified in EC 2.6.1._ or by an amino acid dehydrogenase classified in EC 1.4.1 In particular, it has been reported in the literature that this reaction (in most reports in the reverse direction) is supported by numerous aminotransferases (see, e.g., Bouzon et al. , ACS Synth. Biol. 6 (2017), 1520-1533; Hernandez et al., ACS Catal. 7 (2017), 1707-1711; Walther et al., Metab. Eng. 45 (2018), 237-245; Zhong et al., ACS Synthetic Biology 8 (2019), 587-595) as well as amino acid dehydrogenases such as (engineered) glutamate dehydrogenase (Chen et al., Biotechnol. J. 10 (2015), 284-289).

The use of an aminotransferase classified in EC 2.6.1._ for carrying out the conversion of HOB into homoserine has already been described in the literature, e.g. in Bouzon et al. (ACS Synth. Biol. 6 (2017), 1520-1533).

Examples of aminotransferases classified in EC 2.6.1._ which may be particularly useful in the context of the method/pathway of the present invention are aminotransferases selected from the following:

(i) aminotransferases classified in EC 2.6.1.2 (glutamate-pyruvate aminotransferase; also referred to as alanine transaminase);

(ii) aminotransferases classified in EC 2.6.1.1 (aspartate aminotransferase; also referred to as aspartate transaminase);

(iii) aminotransferases classified in EC 2.6.1.42 (branched-chain amino acid aminotransferase).

Aminotransferases classified in EC 2.6.1.2 (glutamate-pyruvate aminotransferase; also referred to as alanine transaminase) naturally catalyze the reaction:

2-oxoglutarate + L-alanine L-glutamate + pyruvate

This enzyme occurs in a large variety of organism, including eukaryotic and prokaryotic organisms, such as animals, plants, fungi and bacteria. In principle any glutamate-pyruvate aminotransferase (EC 2.6.1.2) can be employed in the method according to the present invention as long as it can convert HOB into homoserine. In a preferred embodiment an enzyme from E. coli is used, in particular an enzyme encoded by the alaC and alaA genes of E. coli (Uniprot accession numbers P77434 and P0A959). Bouzon et al. (ACS Synth. Biol. 6 (2017), 1520-1533) already described the use of a glutamate-pyruvate aminotransferase (EC 2.6.1.2) for the reversible conversion of L-homoserine and 2-oxoglutarate into HOB and L-glutamate. The enzyme from E. coli employed in Bouzon et al. (ACS Synth. Biol. 6 (2017), 1520- 1533) showed substitutions at positions 142 and 275, namely the substitutions A142P and Y275D, which increase the catalytic efficiency of the enzyme for homoserine dramatically. Thus, in a preferred embodiment, a glutamate-pyruvate aminotransferase (EC 2.6.1.2) is employed which shows substitutions at position 142 and/or 275 in the sequence of the E. coli AlaC protein or at positions corresponding to these positions and even more preferably substitutions from A to P at position 142 (or a corresponding position) and/or a substitution from Y to D at position 275 (or a corresponding position).

Aminotransferases classified in EC 2.6.1.1 (aspartate aminotransferase; also referred to as aspartate transaminase) naturally catalyze the reaction:

2-oxoglutarate + L-aspartate L-glutamate + oxalacetate

This enzyme occurs in a large variety of organism, including eukaryotic and prokaryotic organisms, such as animals, plants, fungi and bacteria. In principle any aspartate aminotransferase (EC 2.6.1.1) can be employed in the method according to the present invention as long as it can convert HOB into homoserine. In a preferred embodiment an enzyme from E. coli is used, in particular an enzyme encoded by the aspC gene of E. coli (Uniprot accession number P00509). For this enzyme it has already been reported that it can catalyze this reaction (Walther et al, Metabolic Engin. 45 (2018), 237-245; Zhong et al., ACS Synthetic Biology 8 (2019) 587-595).

Aminotransferases classified in EC 2.6.1.42 (branched-chain amino acid aminotransferase) naturally catalyze the reactions:

2-oxoglutarate + L-leucine 4-methyl-2-oxopentanoate + L-glutamate

2-oxoglutarate + L-valine 3-methyl-2-oxobutanoate + L-glutamate

This enzyme occurs in a large variety of organism, including eukaryotic and prokaryotic organisms, such as animals, plants, fungi and bacteria. In principle any branched-chain amino acid aminotransferase (EC 2.6.1.42) can be employed in the method according to the present invention as long as it can convert HOB into homoserine. In a preferred embodiment an enzyme from E. coli is used, in particular an enzyme encoded by the ilvE gene of E. coli (Uniprot accession number P0AB80). For this enzyme it has already been reported that it can catalyze this reaction (Walther et al, Metabolic Engin. 45 (2018), 237-245).

The use of an amino acid dehydrogenase classified in EC 1.4.1._ for carrying out the conversion of HOB into homoserine has also already been described in the literature, e.g. in Chen et al. (Biotechnol. J. 10 (2015), 284-289). Examples of amino acid dehydrogenases classified in EC 1.4.1 which may be particularly useful in the context of the method/pathway of the present invention are amino acid dehydrogenases classified in EC 1.4.1.4 (glutamate dehydrogenase (NADP⁺)).

Glutamate dehydrogenase (NADP⁺) (EC 1.4.1.4) naturally catalyzes the reaction:

L-glutamate + H₂0 + NADP⁺ 2-oxoglutarate + NH₃ + NADPH + H⁺

This enzyme occurs in a large variety of organism, including eukaryotic and prokaryotic organisms, such as animals, plants, fungi and bacteria. In principle any glutamate dehydrogenase (NADP⁺) (EC 1.4.1.4) can be employed in the method according to the present invention as long as it can convert HOB into homoserine. In a preferred embodiment an enzyme from E. coli is used, in particular an enzyme encoded by the gdhA gene of E. coli (Uniprot accession number P00370).

As reported in Chen et al. (Biotechnol. J. 10 (2015), 284-289), glutamate dehydrogenase (NADP⁺) (EC 1.4.1.4) can accept homoserine as a substrate albeit with a low acitivity. However, Chen et al. (loc. cit.) showed that mutants of this enzyme can be provided which show a strongly increased activity using homoserine as a substrate. Thus, in a preferred embodiment, a mutant version of a glutamate dehydrogenase (NADP⁺) (EC 1.4.1.4) is employed which shows an increased activity in using homoserine as a substrate and, in particular a mutant version as disclosed in Chen et al. (Biotechnol. J. 10 (2015), 284-289).

Step 3 of the method: conversion of homoserine into threonine The conversion of the homoserine produced in step (2) into threonine according to step (3) of the method according to the present invention can be achieved by methods known to the person skilled in the art. In a preferred embodiment, the conversion of the homoserine produced in step (2) into threonine takes place by (i) phosphorylation of thus produced homoserine to produce o- phosphohomoserine; and

(ii) dephosphorylation of the thus produced o-phosphohomoserine to produce threonine.

These are naturally reactions. The reaction of step (i), i.e. the phosphorylation of homoserine to produce o-phosphohomoserine is catalyzed by enzymes classified in EC 2.7.1.39 (homoserine kinase). The reaction proceeds according to the following scheme:

ATP + L-homoserine ADP + o-phospho-L-homoserine

This enzyme occurs in a large variety of organism, including eukaryotic and prokaryotic organisms, such as plants, fungi and bacteria. In principle any homoserine kinase (EC 2.7.1.39) can be employed in the method according to the present invention as long as it can convert homoserine into o-phosphohomoserine. In a preferred embodiment an enzyme from E. coli is used, in particular an enzyme encoded by the thrB gene of E. coli (Uniprot accession number P00547).

The reaction of step (ii), i.e. the dephosphorylation of the produced o- phosphohomoserine to produce threonine is catalyzed by enzymes classified in EC EC 4.2.3.1 (threonine synthase). The reaction proceeds according to the following scheme: o-phospho-L-homoserine + H₂0 L-threonine + phosphate

This enzyme occurs in a large variety of organism, including eukaryotic and prokaryotic organisms, such as plants, fungi and bacteria. In principle any threonine synthase (EC 4.2.3.1) can be employed in the method according to the present invention as long as it can convert o-phosphohomoserine into threonine. In a preferred embodiment an enzyme from E. coli is used, in particular an enzyme encoded by the thrC gene of E. coli (Uniprot accession number P00934).

Step 4 of the method: conversion of threonine into glycine and acetaldehyde In step (4) of the method according to the present invention threonine is converted into glycine. This conversion can be achieved in different ways.

In one embodiment, threonine is converted into glycine and acetaldehyde. This conversion can, e.g., be achieved by using a threonine aldolase. Threonine aldolases are enzymes which convert threonine (or allo-threonine) into glycine and acetaldehyde. Examples for suitable threonine aldolases are threonine aldolases which are classified in EC 4.1.2.5 or EC 4.1.2.48.

Threonine aldolases classified in EC 4.1.2.5 have been identified in a large variety of organism, including eukaryotic and prokaryotic organisms, such as plants, animals and bacteria. In principle any threonine aldolase classified in EC 4.1.2.5 can be employed in the method according to the present invention as long as it can convert threonine into glycine and acetaldehyde.

Threonine aldolases classified in EC 4.1.2.48 (also referred to as low-specificity L- threonine aldolases) have been identified in a large variety of organism, including eukaryotic and prokaryotic organisms, such as fungi and bacteria. In principle any threonine aldolase classified in EC 4.1.2.48 can be employed in the method according to the present invention as long as it can convert threonine into glycine and acetaldehyde. In a preferred embodiment an enzyme from E. coli is used, in particular an enzyme encoded by the ItaE gene of E. coli (Uniprot accession number P75823).

In a preferred embodiment such an enzyme has an amino acid sequence as shown in SEQ ID NO: 5 or shows an amino acid sequence which is at least x% homologous to SEQ ID NO: 3 and has the activity of a low-specificity L-threonine aldolases (EC 4.1.2.48) with x being an integer between 60 and 100, preferably 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98 or 99 wherein such an enzyme is capable of catalyzing the conversion of threonine into glycine and acetaldehyde.

Preferably, the degree of identity is determined by comparing the respective sequence with the amino acid sequence of SEQ ID NO: 5. As regards the determination of the degree of sequence identity the same applies as had been set forth above.

In an alternative embodiment, threonine can be converted in step (5) into glycine and acetyl-CoA. This conversion can, e.g., be achieved by a combination of a threonine dehydrogenase (EC 1.1.1.103) and a 2-amino-3-ketobutyrate CoA ligase (EC 2.3.1.29).

Threonine dehydrogenase (EC 1.1.1.103) naturally catalyzes the following reaction:

L-threonine + NAD⁺ ^=^2-amino-3-oxobutanoate + NADH⁺ + H⁺

Threonine dehydrogenase classified in EC 1.1.1.103 has been identified in a large variety of organism, including eukaryotic and prokaryotic organisms, such as animals and bacteria. In principle any threonine dehydrogenase classified in EC 1.1.1.103 can be employed in the method according to the present invention as long as it can convert threonine into 2-amino-3-oxobutanoate. In a preferred embodiment an enzyme from E. coli is used, in particular an enzyme encoded by the tdh gene of E. coli (Uniprot accession number P07913).

2-amino-3-ketobutyrate CoA ligase (EC 2.3.1.29; also referred to as glycine C- acetyltransferase) naturally catalyzes the following reaction:

2-amino-3-oxobutanoate + CoA glycine + acetyl-CoA

The enzyme has been identified in a large variety of organism, including eukaryotic and prokaryotic organisms, such as animals and bacteria. In principle any 2-amino-3- ketobutyrate CoA ligase classified in EC 2.3.1.29 can be employed in the method according to the present invention as long as it can convert 2-amino-3-oxobutanoate and CoA into glycine and acetyl-CoA. In a preferred embodiment an enzyme from E. coli is used, in particular an enzyme encoded by the kbl gene of E. coli (Uniprot accession number P0AB77).

[Step 5]

According to step (5) of the method of the invention, glycine is condensed with formaldehyde so as to produce serine. This condensation reaction can, e.g., be achieved by making use of a threonine aldolase. As regards the definition of threonine aldolase, the same applies as had already been set forth above in connection with step (4) of the method. Thus, in a preferred embodiment the threonine aldolase used in this step is a threonine aldolase which is classified in EC 4.1.2.5 or EC 4.1.2.48. As regards the preferred embodiments, the same applies as has been set forth above in connection with step (4).

In a particularly preferred embodiment, the threonine aldolase employed in this step is a threonine aldolase classified in EC 4.1.2.48, even more preferably a threonine aldolase from E. coli (Uniprot accession number P75823) encoded by the latE gene. It has been reported in Contestabile et al (Eur. J. Biochem 268 (2001), 6508-6525) that a threonine aldolase can indeed catalyze this condensation of glycine with formaldehyde so as to produce serine.

[Step 6]

According to step (6) of the method of the invention, the serine produced in step (5) is then further converted so as to produce pyruvate. Enzymatic reactions for achieving this conversion and the respective enzymes are known to the person skilled in the art. In one embodiment, the conversion of serine into pyruvate is achieved by a deamination reaction. Enzymes which catalyze this reaction are known to the person skilled in the art in include, e.g., serine deaminases (EC 4.3.1.17; also referred to as L-serine ammonia-lyase) and threonine deaminases (EC 4.3.1.19; also known as threonine ammonia-lyase).

An alternative route for converting serine into pyruvate goes from serine to hydroxypyruvate via an aminotransferase enzyme (2.6.1.X) or an amino acid dehydrogenase enzyme (EC 1.4.1.X). Hydroxypyruvate is then converted into glycerate by a hydroxypyruvate reductase (1.1.1.29 or 1.1.1.81 ), which is then further converted into 2-phosphoglycerate by a glycerate 2-kinase (2.7.1.165). The latter can be replaced by a combination of glycerate 3-kinase (EC 2.7.1.31) and phosphoglycerate mutase (EC 5.4.2.1). Phosphoglycerate can then be converted into phosphoenolpyruvate by an enolase (EC 4.2.1.11), which can then be convertet into pyruvate by pyruvate kinase (EC 2.7.1.40).

The formaldehyde which is used in the condensation steps (1) and (5) of the method according to the invention can be provided by means and methods known to the person skilled in the art. In one embodiment, the formaldehyde is externally provided, i.e. added to the reaction mixture or to the culture medium in which an organism which is suitable for catalyzing the reactions of the method according to the invention is cultured.

In another embodiment, the formaldehyde is itself provided by an enzymatic conversion. The skilled person is aware of various substrates from which formaldehyde can be produced enzymatically. Examples are, e.g., methanol, formate, methane, halogenated methane and methylamine (or a derivative thereof) as well as methylated amino acids (e.g. sarcosine, betaine and glycine).

In a preferred embodiment the formaldehyde is provided by the enzymatic conversion of methanol into formaldehyde, preferably via an oxidation reaction.

One type of enzyme which can be used in this context is methanol dehydrogenase (NAD⁺) which is classified as EC 1.1.1.244. This enzyme catalyzes the reaction:

Methanol + NAD⁺ formaldehyde + NADH + H⁺

One example for such a methanol dehydrogenase which can be employed in a method according to the present invention is the methanol dehydrogenase encoded by the adhA gene of C. glutamicum. For this enzyme it has already been shown in the literature that it can convert methanol into formaldehyde (He et al. , ACS Synthetic Biology 7 (2018), 1601-1611). Another type of enzyme which can be used for the production of formaldehyde from methanol is methanol dehydrogenase (cytochrome c; also referred to as “quinone- dependent”) which is classified as EC 1.1.2.7.

Another type of enzyme which can be used for the production of formaldehyde from methanol is a methanol oxidase, for example, methanol oxidases classified in EC 1.1.3.13

As mentioned above, the formaldehyde used in the condensation steps (1) and (5) of the method of the invention can also be provided by enzymatically producing it from methane. This can, e.g., be achieved by converting methane into methanol which, in turn, can then be converted into formaldehyde as described above. The conversion of methane into methanol can, e.g., be achieved by making use of a methane monooxygenase (EC 1.14.14.3 or 1.14.13.25).

As further mentioned above, the formaldehyde used in the condensation steps (1) and (5) of the method of the invention can also be provided by enzymatically producing it from halogenated methane. This can, e.g., be achieved by converting halogenated methane, e.g. dichloromethane, into formaldehyde. This conversion can, e.g., be achieved by making use of a dehalogenase, preferably a dehalogenase (classified in EC 4.5.1.3).

Another substrate from which formaldehyde may be produced enzymatically is methylamine (or a derivative thereof). Methylamine (or a derivative thereof) can, e.g., be converted into formaldehyde by oxidation. This can be achieved by making, e.g., use of a methylamine dehydrogenase (classified in EC 1.4.9.1) or of a primary amine oxidase (classified in EC 1.4.3.21).

Furthermore, formaldehyde can also be enzymatically provided starting from formate. It has, for example, been described in Siegel et al. (Proc. Natl. Acad. Sci. USA 24 (2015), 3704-3709) that formate can be converted by acetyl-CoA synthase (ACS) into formyl-CoA which can then be further converted into formaldehyde by making use of an acetaldehyde dehydrogenase (ACDH).

A method according to the present invention may be carried out in vitro or in vivo. An in vitro reaction is understood to be a reaction in which no cells are employed, i.e. an acellular reaction. Thus, in vitro preferably means in a cell-free system. The term “in vitro” in one embodiment means in the presence of isolated enzymes (or enzyme systems optionally comprising possibly required cofactors). In one embodiment, the enzymes employed in the method are used in purified form. For carrying out the method in vitro the substrates for the reaction and the enzymes are incubated under conditions (buffer, temperature, cosubstrates, cofactors etc.) allowing the enzymes to be active and the enzymatic conversion to occur. The reaction is allowed to proceed for a time sufficient to produce the respective product. The production of the respective products can be measured by methods known in the art, such as gas chromatography possibly linked to mass spectrometry detection.

The enzymes may be in any suitable form allowing the enzymatic reaction to take place. They may be purified or partially purified or in the form of crude cellular extracts or partially purified extracts. It is also possible that the enzymes are immobilized on a suitable carrier.

In another embodiment the method according to the invention is carried out in culture, in the presence of an organism, preferably a microorganism, producing enzymes described above for the conversions of the method according to the present invention as described herein above.

A method which employs a microorganism for carrying out a method according to the invention is referred to as an “in vivo” method. It is possible to use a microorganism which naturally produces the enzymes described above for the conversions of the method according to the present invention or a microorganism which had been genetically modified so that it expresses (including overexpresses) one or more of such enzymes.

In one embodiment, the microorganism used in a method of the invention is a microorganism which naturally expresses enzymes which catalyze steps (2), (3), (4) and (6) of the method of the invention and in which enzymes catalyzing steps (1) and (5) are overexpressed.

Thus, the microorganism can be an engineered microorganism which expresses at least some of the enzymes described above for the conversions of the method according to the present invention, i.e. which has in its genome (or on a plasmid) a nucleotide sequence encoding such enzymes and which has been modified to overexpress them. The expression may occur constitutively or in an induced or regulated manner.

In another embodiment the microorganism can be a microorganism which has been genetically modified by the introduction of one or more nucleic acid molecules containing nucleotide sequences encoding one or more enzymes described above for the conversions of the methods according to the present invention. The nucleic acid molecule can be stably integrated into the genome of the microorganism or may be present in an extrachromosomal manner, e.g. on a plasmid. Such a genetically modified microorganism can, e.g., be a microorganism that does not naturally express all enzymes described above for the conversions of the method according to the present invention and which has been genetically modified to express such enzymes or a microorganism which naturally expresses such enzymes and which has been genetically modified, e.g. transformed with a nucleic acid, e.g. a vector, encoding the respective enzyme(s), and/or insertion of a promoter in front of the endogenous nucleotide sequence encoding the enzyme(s) or the introduction of an efficient ribosomal binding site in order to increase the respective activity in said microorganism.

However, the invention preferably excludes naturally occurring microorganisms as found in nature expressing the enzymes as described above at levels as they exist in nature. Instead, the microorganism of the present invention and employed in a method of the present invention is preferably a non-naturally occurring microorganism, whether it has been genetically modified to express (including overexpression) at least one exogenous nucleic acid molecule not normally existing in its genome.

In one embodiment the (micro)organisms employed in connection with the present invention are preferably non-naturally occurring (micro)organisms, i.e. they are (micro)organisms which differ significantly from naturally occurring or (micro)organism and which do not occur in nature.

As regards the enzymes, they can be naturally occurring enzymes or they can be variants of naturally occurring enzymes which do not as such occur in nature. Such variants include, for example, mutants, in particular prepared by molecular biological methods, which show improved properties, such as a higher enzyme activity, higher substrate specificity, higher temperature resistance and the like. As regards the (micro)organisms, they are preferably genetically modified organisms as described herein which differ from naturally occurring organisms due to a genetic modification. Genetically modified organisms are organisms which do not naturally occur, i.e., which cannot be found in nature, and which differ substantially from naturally occurring organisms due to the introduction of a foreign nucleic acid molecule.

By overexpressing an exogenous or endogenous enzyme as described herein above, the concentration of the enzyme is substantially higher than what is found in nature. Such overexpression can lead to the direction of the metabolic flux in a correspondingly genetically modified microorganism into a certain direction. Such an overexpression can also force the reaction(s) of the method of the present invention which uses a non-natural substrate for the respective enzyme into a certain direction. Preferably, the term “overexpressed” means that the activity of the corresponding enzyme is at least 5%, 10%, 20%, 30% or 40% higher in a genetically modified microorganism expressing said enzyme than in a corresponding non-genetically modified microorganism.

Some of the steps of the method of the present invention (e.g. steps (1 ), (2) and (5)) rely on conversions which are understood not to be catalyzed by the respective enzymes according to their “natural” reaction. This means that the enzyme is known to naturally catalyze a different reaction, in particular uses a different substrate. The present invention makes use of the fact that it was found that certain enzymes are also able to use “non-natural” substrates and catalyze the respective conversion. The ability of an enzyme to use a “non-natural” substrate is also referred to as a “promiscuous” activity. A “non-natural” substrate is understood to be a molecule that is not acted upon by the respective enzyme in nature (or only to a minor degree), even though it may actually coexist in the microorganism along with the endogenous enzyme. This “non-natural” substrate is not converted by the microorganism in nature (or only to a minor degree) as other substrates are preferred (e.g. the “natural substrate”). Thus, the present invention contemplates utilizing for certain steps enzymes which can accept a non-natural substrate.

It is also possible in the context of the present invention that the microorganism is a microorganism which naturally does not have one or more of the enzyme activities required by the method of the invention but which is genetically modified so as to comprise (a) nucleotide sequence(s) allowing the expression of (a) corresponding enzyme(s). Similarly, the microorganism may also be a microorganism which naturally has the respective enzyme activity/activities but which is genetically modified so as to enhance such (an) activity/activities, e.g. by the introduction of (an) exogenous nucleotide sequences encoding (a) corresponding enzyme(s) or by the introduction of a promoter for the endogenous gene(s) encoding the enzyme(s) to increase endogenous production to overexpressed (non-natural) levels.

If a microorganism is used which naturally expresses (a) corresponding enzyme(s), it is possible to modify such a microorganism so that the respective activity//activities is(are) overexpressed in the mircroorganism. This can, e.g., be achieved by effecting mutations in the promoter region of the corresponding gene(s) or introduction of a high expressing promoter so as to lead to a promoter which ensures a higher expression of the gene(s). Alternatively, it is also possible to mutate the gene(s) as such so as to lead to an enzyme showing a higher activity.

By using microorganisms which express enzymes described above for the conversions of the methods according to the present invention, it is possible to carry out the methods according to the invention directly in the culture medium, without the need to separate or purify the enzymes. In one embodiment the organism employed in a method according to the invention is a microorganism which has been genetically modified to contain a foreign nucleic acid molecule encoding at least one enzyme described above for the conversions of the methods according to the present invention.

In one embodiment the microorganism has been genetically modified to contain a foreign nucleic acid molecule encoding an enzyme which can catalyze the conversion of step (1) of the method according to the present invention.

In one embodiment the microorganism has been genetically modified to contain a foreign nucleic acid molecule encoding an enzyme which can catalyze the conversion of step (1 ) of the method according to the present invention and has been genetically modified to contain a foreign nucleic acid molecule encoding an enzyme which can catalyze the conversion of step (5) of the method according to the present invention.

The term “foreign” or “exogenous” in this context means that the nucleic acid molecule does not naturally occur in said microorganism. This means that it does not occur in the same structure or at the same location in the microorganism. In one preferred embodiment, the foreign nucleic acid molecule is a recombinant molecule comprising a promoter and a coding sequence encoding the respective enzyme in which the promoter driving expression of the coding sequence is heterologous with respect to the coding sequence. “Heterologous” in this context means that the promoter is not the promoter naturally driving the expression of said coding sequence but is a promoter naturally driving expression of a different coding sequence, i.e. , it is derived from another gene, or is a synthetic promoter or a chimeric promoter. Preferably, the promoter is a promoter heterologous to the microorganism, i.e. a promoter which does naturally not occur in the respective microorganism. Even more preferably, the promoter is an inducible promoter. Promoters for driving expression in different types of organisms, in particular in microorganisms, are well known to the person skilled in the art.

In a further embodiment the nucleic acid molecule is foreign to the microorganism in that the encoded enzyme is not endogenous to the microorganism, i.e. is naturally not expressed by the microorganism when it is not genetically modified. In other words, the encoded enzyme is heterologous with respect to the microorganism. The foreign nucleic acid molecule may be present in the microorganism in extrachromosomal form, e.g. as a plasmid, or stably integrated in the chromosome. A stable integration is preferred. Thus, the genetic modification can consist, e.g. in integrating the corresponding gene(s) encoding the enzyme(s) into the chromosome, or in expressing the enzyme(s) from a plasmid containing a promoter upstream of the enzyme-coding sequence, the promoter and coding sequence preferably originating from different organisms, or any other method known to one of skill in the art. The term “microorganism” in the context of the present invention refers to bacteria, as well as to fungi, such as yeasts, and also to algae and archaea. In one preferred embodiment, the microorganism is a bacterium. In principle any bacterium can be used. Preferred bacteria to be employed in the process according to the invention are bacteria of the genus Bacillus, Clostridium, Corynebacterium, Pseudomonas, Zymomonas or Escherichia. In a particularly preferred embodiment the bacterium belongs to the genus Escherichia and even more preferred to the species Escherichia coli. In another preferred embodiment the bacterium belongs to the species Pseudomonas putida or to the species Zymomonas mobilis or to the species Corynebacterium glutamicum or to the species Bacillus subtilis.

It is also possible to employ an extremophilic bacterium such as Thermus thermophilus, or anaerobic bacteria from the family Clostridiae.

In another preferred embodiment the microorganism is a fungus, more preferably a fungus of the genus Saccharomyces, Schizosaccharomyces, Aspergillus, Trichoderma, Kluyveromyces or Pichia and even more preferably of the species Saccharomyces cerevisiae, Schizosaccharomyces pombe, Aspergillus niger, Trichoderma reesei, Kluyveromyces marxianus, Kluyveromyces lactis, Pichia pastoris, Pichia torula or Pichia utilis.

In another embodiment, the method according to the invention makes use of a photosynthetic microorganism expressing at least one enzyme for the conversion according to the invention as described above. Preferably, the microorganism is a photosynthetic bacterium, or a microalgae. In a further embodiment the microorganism is an algae, more preferably an algae belonging to the diatomeae.

In another embodiment the method according to the invention makes use of a microorganism which is able to metabolize methanol, e.g. a methanothrophic bacterium, a methylotrophic bacterium, a methanothrophic yeast or a methylotrophic yeast.

In another embodiment, the microorganism is a C1 -fixing microorganism, preferably a recombinant C1 -fixing microorganism. The nature of the C1 -fixing microorganism is not particularly limited as long as it is a microorganism which is capable using carbon monoxide (CO) and gaseous substrates comprising CO like, e.g., syngas, as the source of carbon and energy. Syngas or synthesis gas is a mixture of CO and CO2 as well as H_2. Corresponding naturally occuring (or genetically modified) microorganisms are known in the art that are capable of utilizing CO and converting it into acetyl-CoA. These organisms are often referred to as acetogenic microorganisms (sometimes also termed carboxydotrophic, acetogenic microorganisms). These microorganisms use the Wood-Ljungdahl pathway to fix CO and convert it into acetyl-CoA. Examples of such microorganisms belong to the family Clostridiae and are, e.g., described in WO 2009/094485; WO 2012/05905; WO 2013/180584; US 2011/0236941; PNAS 107(29): 13087-13092 (2010); Current Opinion in Biotechnology 23:364-381 (2012); Applied and Environmental

Microbiology 77(15):5467-5475 (2011).

In certain embodiments, the C1 -fixing microorganism is selected from the group of carboxydotrophic acetogenic bacteria. In preferred embodiments, the microorganism is selected from the group comprising Clostridium autoethanogenum, Clostridium ljungdahlii, Clostridium ragsdalei, Clostridium carboxidivorans, Clostridium drakei, Clostridium scatologenes, Clostridium aceticum, Clostridium formicoaceticum, Clostridium magnum, Butyribacterium methylotrophicum, Acetobacterium woodii, Alkalibaculum bacchii, Blautia producta, Eubacterium limosum, Moorella thermoacetica, Moorella thermautotrophica, Sporomusa ovata, Sporomusa silvacetica, Sporomusa sphaeroides, Oxobacter pfennigii, and Thermoanaerobacter kiuvi.

In order to improve flux through the “homoserine cycle” when implemented in a microorganism, it may be advantageous to inactivate certain enzymatic activities which may endogenously occur in the respective microorganism.

Thus, in one embodiment, the microorganism in which the method of the invention is implemented is a microorganism which endogenously contains an operon encoding a formaldehyde detoxification system and in which this operon is deleted or rendered inactive. In a preferred embodiment, the microorganism belongs to the species E. coli and is a microorganism in which the formaldehyde detoxification ( frmRAB ) operon (Chen et al. , Metabol. Engineering 49 (2018), 257-266) is deleted or rendered inactive. This system is also referred to as the glutathione-dependent formaldehyde oxidation system. The inactivation or deletion of this operon ensures that formaldehyde is not removed and is available for cell growth. In particular, the inactivation of this operon should avoid formaldehyde oxidation to formate which may lead to a depletion of the intracellular pool of formaldehyde.

In another embodiment, the microorganism in which the method of the invention is implemented is a microorganism which endogenously contains a gene(s) encoding (an) enzyme activity/activities which catalyze(s) the conversion of pyryvate into aspartate semialdehyde and in which this/these gene(s) is/are deleted or rendered inactive. In a preferred embodiment said gene is a gene encoding aspartate semialdehyde dehydrogenase (EC 1.2.1.11). In a preferred embodiment, the microorganism belongs to the species E. coli and is a microorganism in which the asd gene encoding said enzyme is deleted or rendered inactive.

In another embodiment, the microorganism in which the method of the invention is implemented is a microorganism which endogenously contains a gene encoding a 3- phosphoglycerate dehydrogenase (EC 1.1.1.95) and in which this gene is deleted or rendered inactive. In a preferred embodiment, the microorganism belongs to the species E. coli and is a microorganism in which the serA gene encoding said enzyme is deleted or rendered inactive.

In another embodiment, the microorganism in which the method of the invention is implemented is a microorganism which endogenously contains a gene encoding a serine hydroxymethyltransferase (EC 2.1.2.1) and in which this gene is deleted or rendered inactive. In a preferred embodiment, the microorganism belongs to the species E. coli and is a microorganism in which the glyA gene encoding said enzyme is deleted or rendered inactive.

In another embodiment, the microorganism in which the method of the invention is implemented is a microorganism which endogenously contains an operon encoding a glycin cleavage system (GCS) and in which this operon is deleted or rendered inactive. In a preferred embodiment, the microorganism belongs to the species E. coli and is a microorganism in which the glycine cleavage system ( gcvTHP ) operon is deleted or rendered inactive. The GCS operon in E. coli encodes for three enzymes, i.e. GcvT, GcvH and GcvP, and it is either possible to inactive the whole operon or one or more of the three genes.

In another embodiment, the microorganism in which the method of the invention is implemented is a microorganism which endogenously contains a gene encoding a bifunctional aspartokinase/homoserine dehydrogenase (EC 1.1.1.3) and in which this gene is deleted or rendered inactive. In a preferred embodiment, the microorganism belongs to the species E. coli and is a microorganism in which the thrA gene encoding said enzyme is deleted or rendered inactive.

In another embodiment, the microorganism in which the method of the invention is implemented is a microorganism which endogenously contains a gene encoding an L-threonine 3-dehydrogenase dehydrogenase (EC 1.1.1.103) and in which this gene is deleted or rendered inactive. In a preferred embodiment, the microorganism belongs to the species E. coli and is a microorganism in which the tdh gene encoding said enzyme is deleted or rendered inactive.

In another embodiment, the microorganism in which the method of the invention is implemented is a microorganism which endogenously contains a gene encoding a 2- amino-3-ketobutyrate CoA ligase (EC 2.3.1.29) and in which this gene is deleted or rendered inactive. In a preferred embodiment, the microorganism belongs to the species E. coli and is a microorganism in which the kbl gene encoding said enzyme is deleted or rendered inactive.

In another embodiment, the microorganism in which the method of the invention is implemented is a microorganism which endogenously contains a gene encoding a lactate dehydrogenase (EC 1.1.1.28) and in which this gene is deleted or rendered inactive. In a preferred embodiment, the microorganism belongs to the species E. coli and is a microorganism in which the IdhA gene encoding said enzyme is deleted or rendered inactive.

In another embodiment the microorganism shows any possible combination of gene deletions as described above.

It is also conceivable to use in the method according to the invention a combination of microorganisms wherein different microorganisms express different enzymes as described above. The genetic modification of microorganisms to express an enzyme of interest will also be further described in detail below.

In another embodiment, the method of the invention comprises the step of providing the organism, preferably the microorganism carrying the respective enzyme activity or activities in the form of a (cell) culture, preferably in the form of a liquid cell culture, a subsequent step of cultivating the organism, preferably the microorganism in a fermenter (often also referred to a bioreactor) under suitable conditions allowing the expression of the respective enzyme and further comprising the step of effecting an enzymatic conversion of a method of the invention as described herein above. Suitable fermenter or bioreactor devices and fermentation conditions are known to the person skilled in the art. A bioreactor or a fermenter refers to any manufactured or engineered device or system known in the art that supports a biologically active environment. Thus, a bioreactor or a fermenter may be a vessel in which a chemical/biochemical like the method of the present invention is carried out which involves organisms, preferably microorganisms and/or biochemically active substances, i.e. , the enzyme(s) described above derived from such organisms or organisms harbouring the above described enzyme(s). In a bioreactor or a fermenter, this process can either be aerobic or anaerobic. These bioreactors are commonly cylindrical, and may range in size from litres to cubic metres, and are often made of stainless steel. In this respect, without being bound by theory, the fermenter or bioreactor may be designed in a way that it is suitable to cultivate the organisms, preferably microorganisms, in, e.g., a batch-culture, fed-batch-culture, perfusion culture or chemostate-culture, all of which are generally known in the art.

The culture medium can be any culture medium suitable for cultivating the respective organism or microorganism.

When carried out by making use of a microorganism, the method according to the present invention may, e.g. be designed as a continuous fermentation culturing method or as a batch culture or any suitable culture method known to the person skilled in the art. The enzymes used in the method according to the invention can be a naturally occurring enzymes or enzymes which are derived from a naturally occurring enzymes, e.g. by the introduction of mutations or other alterations which, e.g., alter or improve the enzymatic activity, the stability, etc.

Methods for modifying and/or improving the desired enzymatic activities of proteins are well-known to the person skilled in the art and include, e.g., random mutagenesis or site-directed mutagenesis and subsequent selection of enzymes having the desired properties or approaches of the so-called “directed evolution”.

For example, for genetic modification in prokaryotic cells, a nucleic acid molecule encoding a corresponding enzyme can be introduced into plasmids which permit mutagenesis or sequence modification by recombination of DNA sequences. Standard methods (see Sambrook and Russell (2001), Molecular Cloning: A Laboratory Manual, CSH Press, Cold Spring Harbor, NY, USA) allow base exchanges to be performed or natural or synthetic sequences to be added. DNA fragments can be ligated by using adapters and linkers complementary to the fragments. Moreover, engineering measures which provide suitable restriction sites or remove surplus DNA or restriction sites can be used. In those cases, in which insertions, deletions or substitutions are possible, in vitro mutagenesis, “primer repair”, restriction or ligation can be used. In general, a sequence analysis, restriction analysis and other methods of biochemistry and molecular biology are carried out as analysis methods. The resulting enzyme variants are then tested for the desired activity, e.g., enzymatic activity, with an assay as described above and in particular for their increased enzyme activity.

As described above, the microorganism employed in a method of the invention may be a microorganism which has been genetically modified by the introduction of a nucleic acid molecule encoding a corresponding enzyme. Preferably, the microorganism is a microorganism according to the present invention as described herein below. Thus, in a preferred embodiment, the microorganism is a recombinant microorganism which has been genetically modified to have an increased activity of at least one enzyme described above for the conversions of the method according to the present invention. This can be achieved e.g. by transforming the microorganism with a nucleic acid encoding a corresponding enzyme. Preferably, the nucleic acid molecule introduced into the microorganism is a nucleic acid molecule which is heterologous with respect to the microorganism, i.e. it does not naturally occur in said microorganism.

In the context of the present invention, an “increased activity” preferably means that the expression and/or the activity of an enzyme in the genetically modified microorganism is at least 10%, preferably at least 20%, more preferably at least 30% or 50%, even more preferably at least 70% or 80% and particularly preferred at least 90% or 100% higher than in the corresponding non-modified microorganism. In even more preferred embodiments the increase in expression and/or activity may be at least 150%, at least 200% or at least 500%. In particularly preferred embodiments the expression is at least 10-fold, more preferably at least 100-fold and even more preferred at least 1000-fold higher than in the corresponding non-modified microorganism.

The term “increased” expression/activity also covers the situation in which the corresponding non-modified microorganism does not express a corresponding enzyme so that the corresponding expression/activity in the non-modified microorganism is zero. Preferably, the concentration of the overexpressed enzyme is at least 5%, 10%, 20%, 30%, or 40% of the total host cell protein.

Methods for measuring the level of expression of a given protein in a cell are well known to the person skilled in the art. In one embodiment, the measurement of the level of expression is done by measuring the amount of the corresponding protein. Corresponding methods are well known to the person skilled in the art and include Western Blot, ELISA etc. In another embodiment the measurement of the level of expression is done by measuring the amount of the corresponding RNA. Corresponding methods are well known to the person skilled in the art and include, e.g., Northern Blot.

In the context of the present invention the term “recombinant” means that the microorganism is genetically modified so as to contain a nucleic acid molecule encoding an enzyme as defined above as compared to a wild-type or non-modified microorganism. A nucleic acid molecule encoding an enzyme as defined above can be used alone or as part of a vector.

The nucleic acid molecules can further comprise expression control sequences operably linked to the polynucleotide comprised in the nucleic acid molecule. The term “operatively linked” or "operably linked", as used throughout the present description, refers to a linkage between one or more expression control sequences and the coding region in the polynucleotide to be expressed in such a way that expression is achieved under conditions compatible with the expression control sequence.

Expression comprises transcription of the heterologous DNA sequence, preferably into a translatable mRNA. Regulatory elements ensuring expression in fungi as well as in bacteria, are well known to those skilled in the art. They encompass promoters, enhancers, termination signals, targeting signals and the like. Examples are given further below in connection with explanations concerning vectors.

Promoters for use in connection with the nucleic acid molecule may be homologous or heterologous with regard to its origin and/or with regard to the gene to be expressed. Suitable promoters are for instance promoters which lend themselves to constitutive expression. However, promoters which are only activated at a point in time determined by external influences can also be used. Artificial and/or chemically inducible promoters may be used in this context.

The vectors can further comprise expression control sequences operably linked to said polynucleotides contained in the vectors. These expression control sequences may be suited to ensure transcription and synthesis of a translatable RNA in bacteria or fungi.

In addition, it is possible to insert different mutations into the polynucleotides by methods usual in molecular biology (see for instance Sambrook and Russell (2001), Molecular Cloning: A Laboratory Manual, CSH Press, Cold Spring Harbor, NY, USA), leading to the synthesis of polypeptides possibly having modified biological properties. The introduction of point mutations is conceivable at positions at which a modification of the amino acid sequence for instance influences the biological activity or the regulation of the polypeptide.

Moreover, mutants possessing a modified substrate or product specificity can be prepared. Preferably, such mutants show an increased activity. Alternatively, mutants can be prepared the catalytic activity of which is abolished without losing substrate binding activity.

Furthermore, the introduction of mutations into the polynucleotides encoding an enzyme as defined above allows the gene expression rate and/or the activity of the enzymes encoded by said polynucleotides to be reduced or increased.

For genetically modifying bacteria or fungi, the polynucleotides encoding an enzyme as defined above or parts of these molecules can be introduced into plasmids which permit mutagenesis or sequence modification by recombination of DNA sequences. Standard methods (see Sambrook and Russell (2001), Molecular Cloning: A Laboratory Manual, CSH Press, Cold Spring Harbor, NY, USA) allow base exchanges to be performed or natural or synthetic sequences to be added. DNA fragments can be connected to each other by applying adapters and linkers to the fragments. Moreover, engineering measures which provide suitable restriction sites or remove surplus DNA or restriction sites can be used. In those cases, in which insertions, deletions or substitutions are possible, in vitro mutagenesis, “primer repair”, restriction or ligation can be used. In general, a sequence analysis, restriction analysis and other methods of biochemistry and molecular biology are carried out as analysis methods.

Thus, in accordance with the present invention a recombinant microorganism can be produced by genetically modifying fungi or bacteria comprising introducing the above-described polynucleotides, nucleic acid molecules or vectors into a fungus or bacterium. The polynucleotide encoding the respective enzyme is expressed so as to lead to the production of a polypeptide having any of the activities described above. An overview of different expression systems is for instance contained in Methods in Enzymology 153 (1987), 385-516, in Bitter et al. (Methods in Enzymology 153 (1987), 516-544) and in Sawers et al. (Applied Microbiology and Biotechnology 46 (1996), 1-9), Billman-Jacobe (Current Opinion in Biotechnology 7 (1996), 500-4), Hockney (Trends in Biotechnology 12 (1994), 456-463), Griffiths et al., (Methods in Molecular Biology 75 (1997), 427-440). An overview of yeast expression systems is for instance given by Hensing et al. (Antonie van Leuwenhoek 67 (1995), 261-279), Bussineau et al. (Developments in Biological Standardization 83 (1994), 13-19), Gellissen et al. (Antonie van Leuwenhoek 62 (1992), 79-93, Fleer (Current Opinion in Biotechnology 3 (1992), 486-496), Vedvick (Current Opinion in Biotechnology 2 (1991), 742-745) and Buckholz (Bio/Technology 9 (1991), 1067-1072).

Expression vectors have been widely described in the literature. As a rule, they contain not only a selection marker gene and a replication-origin ensuring replication in the host selected, but also a bacterial or viral promoter, and in most cases a termination signal for transcription. Between the promoter and the termination signal there is in general at least one restriction site or a polylinker which enables the insertion of a coding DNA sequence. The DNA sequence naturally controlling the transcription of the corresponding gene can be used as the promoter sequence, if it is active in the selected host organism. However, this sequence can also be exchanged for other promoter sequences. It is possible to use promoters ensuring constitutive expression of the gene and inducible promoters which permit a deliberate control of the expression of the gene. Bacterial and viral promoter sequences possessing these properties are described in detail in the literature. Regulatory sequences for the expression in microorganisms (for instance E. coli, S. cerevisiae) are sufficiently described in the literature. Promoters permitting a particularly high expression of a downstream sequence are for instance the T7 promoter (Studier et al., Methods in Enzymology 185 (1990), 60-89), lacUV5, trp, trp-lacUV5 (DeBoer et al., in Rodriguez and Chamberlin (Eds), Promoters, Structure and Function; Praeger, New York, (1982), 462-481; DeBoer et al., Proc. Natl. Acad. Sci. USA (1983), 21-25), Ip1, rac (Boros et al., Gene 42 (1986), 97-100). Inducible promoters are preferably used for the synthesis of polypeptides. These promoters often lead to higher polypeptide yields than do constitutive promoters. In order to obtain an optimum amount of polypeptide, a two-stage process is often used. First, the host cells are cultured under optimum conditions up to a relatively high cell density. In the second step, transcription is induced depending on the type of promoter used. In this regard, a tac promoter is particularly suitable which can be induced by lactose or IPTG (=isopropyl43_>-D-thiogalactopyranoside) (deBoer et al., Proc. Natl. Acad. Sci. USA 80 (1983), 21-25). Termination signals for transcription are also described in the literature.

The transformation of the host cell with a polynucleotide or vector as described above can be carried out by standard methods, as for instance described in Sambrook and Russell (2001), Molecular Cloning: A Laboratory Manual, CSH Press, Cold Spring Harbor, NY, USA; Methods in Yeast Genetics, A Laboratory Course Manual, Cold Spring Harbor Laboratory Press, 1990. The host cell is cultured in nutrient media meeting the requirements of the particular host cell used, in particular in respect of the pH value, temperature, salt concentration, aeration, antibiotics, vitamins, trace elements etc.

The present invention furthermore relates to a recombinant microorganism expressing enzymes for catalyzing the following reactions:

(1) condensation of pyruvate with formaldehyde into 4-hydroxy-2-oxobutanoic acid (HOB) by an aldolase classified in EC 4.1.2._;

(2) amination of the thus produced 4-hydroxy-2-oxobutanoic acid (HOB) to produce homoserine by an aminotransferase enzyme classified in EC 2.6.1._ or by an amino acid dehydrogenase (EC 1.4.1._);

(3) phosphorylation of thus produced homoserine to produce o- phosphohomoserine by a homoserine kinase (EC 2.7.1.39);

(4) dephosphorylation of the thus produced o-phosphohomoserine to produce threonine by a threonine synthase (EC 4.2.3.1);

(5) conversion of the thus produced threonine into glycine by a threonine aldolase (selected from the group consisting of EC 4.1.2.5, EC 4.1.2.6, EC 4.1.2.48 and EC 4.1.2.49) or by a combination of a threonine dehydrogenase (EC 1.1.1.103) and a 2-amino-3-ketybutyrate CoA ligase (EC 2.3.1.29);

(6) condensation of the thus produced glycine with formaldehyde to produce serine by a threonine aldolase (selected from the group consisting of EC 4.1.2.5, EC 4.1.2.6, EC 4.1.2.48 and EC 4.1.2.49); and

(7) deamination of the thus produced serine to produce pyruvate by a serine deaminase (EC 4.3.1.17) or a threonine deaminase (EC 4.3.1.19), wherein said pyruvate can then be used as a substrate in step (1 ), wherein said microorganism contains at least one heterologous nucleic acid molecule encoding the aldolase catalyzing step (1) and overexpresses the enzyme catalyzing step (6), i.e. the condensation of glycine with formaldehyde to form serine.

In a preferred embodiment, the microorganism further overexpresses at least one of the enzymes catalyzing step (3), step (4) or step (5). As regards the enzymes mentioned in items (1) to (7) above and preferred embodiments thereof and as regards the preferred embodiments of the microorganism, the same applies as has been described above in connection with the method according to the invention.

The microorganism of the present invention may be a microorganism which is recombinant for all of the enzymatic activites listed in items (1) to (7) above, wherein the term “recombinant” means that the microorganism is transformed with heterologous nucleic acid molecules encoding the respective enzymes. These heterologous nucleic acid molecules may be integrated into the genome of the microorganisms or may be present as extrachromosomal elements. Chromosoaml location is preferred. Thus, in such a case the “homoserine cycle” is established in the respective microorganism by introducing for all the required enzymatic activities the corresponding nucleic acid molecules encoding them. The enzymes encoded by the nucleic acid molecules may be enzymes which endogenously occur in the microorganism but are overexpressed due to the use of heterologous regulatory regions such as heterologous promoters and/or ribosomal binding sites. Alternatively, the enzymes may be enzymes which do not naturally occur in the respective microorganism.

However, the “homoserine cycle” described in the present application shows the advantage that it relies in part on enzymatic activities which are expressed endogenously in a large variety of microorganisms and in particular to such a level that they are able to support the metabolic flux through the cycle. Accordingly, the microorganism according to the present invention may also be a microorganism in which one or more of the enzymatic activities of items (1) to (7), above, occur endogenously. However, in such a case the enzyme activity of item (1) is recombinantly introduced into such an organism and, if it already endogenously occurs in such a microorganism, it is overexpressed. Moreover, it is preferred that one or more of endogenously occurring enzymatic activities listed in items (2) to (7) are overexpressed in the corresponding microorganism. The term “overexpression” means that the enzymatic activity of the respective enzyme is increased in the microorganism in comparison to the parent strain and in particular in comparison to the expression level of the endogenously occurring gene, preferably by at least 5%, 10%, 20%, 30% or by at least 40%. Such an increase may be due to an increase of expression of the corresponding gene. Such an increase of expression can, e.g., be achieved by placing the coding sequence of the respective gene under the control of a heterologous promoter, in particular a heterologous promoter which ensures a higher expression of the gene. Alternatively, the endogenous promoter of the gene can be mutated so as to lead to a higher expression of the gene. Such a promoter replacement or mutation can be effected in the endogenous gene located on the chromosome of the microorganism or the corresponding modified gene with a stronger promoter can be placed on a plasmid. An example for a strong promoter which can be used to allow for the overexpression of the aldolase gene is the constitutive strong promoter pgi-20 (Braatsch et al. , Biotechniques 45 (2008), 335- 337).

In addition, in order to allow for an increased expression, it can also be advantageous to use an efficient ribosome binding site. In this respect, the term “heterologous” also includes the situation in which the respective gene is modified so as to comprise a ribosome binding site which is different from its natural ribosomal binding site and which allows for an increased expression (translation) of the gene. An example of a suitable binding site is the ribosome binding site “C” ( AAGTT AAG AGG C AAG A) (Zelcbuch et al., Nucleic Acids Res. 41 (2013), e98). Another possibility for obtaining an increased enzymatic activity is the expression of a mutated enzyme which shows an increased activity for the respective reaction in comparison to the non-mutated enzyme.

The term “increase of expression” also covers the possibility that the activity of the enzyme is increased due to a mutation in the protein which leads to a higher activity. Means and methods for improving enzyme activity by way of mutation have been described above.

In one preferred embodiment, the microorganism is a microorganism which endogenously expresses a homoserine kinase (EC 2.7.1.39) and in which the homoserine kinase (EC 2.7.1.39) is overexpressed in comparison to the parent strain.

In another preferred embodiment, the microorganism is a microorganism which endogenously expresses a threonine synthase (EC 4.2.3.1) and in which the threonine synthase (EC 4.2.3.1) is overexpressed in comparison to the parent strain. In a further preferred embodiment, the microorganism is a microorganism which endogenously expresses a threonine aldolase (selected from the group consisting of EC 4.1.2.5, EC 4.1.2.6, EC 4.1.2.48 and EC 4.1.2.49) and in which a threonine aldolase (selected from the group consisting of EC 4.1.2.5, EC 4.1.2.6, EC 4.1.2.48 and EC 4.1.2.49) is overexpressed in comparison to the parent strain.

In another preferred embodiment, the microorganism is a microorganism which endogenously expresses a threonine dehydrogenase (EC 1.1.1.103) and a 2-amino- 3-ketybutyrate CoA ligase (EC 2.3.1.29) and in which the threonine dehydrogenase (EC 1.1.1.103) and the 2-amino-3-ketybutyrate CoA ligase (EC 2.3.1.29) is overexpressed in comparison to the parent strain. In another preferred embodiment, the microorganism is a microorganism which endogenously expresses an aminotransferase enzyme classified in EC 2.6.1._ or an amino acid dehydrogenase (EC 1.4.1._) which can convert 4-hydroxy-2-oxobutanoic acid (HOB) into homoserine and in which said aminotransferase enzyme classified in EC 2.6.1._ or said amino acid dehydrogenase (EC 1.4.1._) is overexpressed in comparison to the parent strain.

In another preferred embodiment, the microorganism is a microorganism which endogenously expresses a serine deaminase (EC 4.3.1.17) or a threonine deaminase (EC 4.3.1.19) and in which the serine deaminase (EC 4.3.1.17) or the threonine deaminase (EC 4.3.1.19) is overexpressed in comparison to the parent strain.

In another embodiment, the microorganism overexpresses any possible combination of enzymes mentioned above for catalyzing steps (2) to (7). In a preferred embodiment, the microorganism overexpresses enzymes catalyzing steps (3) and (4), or overexpresses enzymes catalyzing steps (3) and (5), or overexpresses enzymes catalyzing steps (3) and (5), or overexpresses enzymes catalyzing steps (3), (4) and (5).

The microorganism may be a microorganism which endogenously expresses all the enzyme activities as specified in item (1) to (7) above but which is recombinant for at least the enzyme activity specified in item (1), i.e. an aldolase classified in EC 4.1.2._. The term “recombinant” in this context means that the microorganism contains a heterologous nucleic acid molecule encoding the corresponding aldolase. In this context, the aldolase itself may be endogenous to the microorganism but the term “heterologous” in this case means that the aldolase is encoded by a nucleic acid where the coding region is not in its natural context. Preferably, the coding region is either not located at the genomic location where it is naturally located (i.e. it is located at a different position in the genome or it is located on an extrachromosomal element such as a plasmid) and/or, it is linked to heterologous regulatory regions, such as a promoter. It is preferred that the gene encoding the aldolase is modified in such a manner that its expression is increased in comparison to the expression of the corresponding endogenous gene, preferably by at least 5%, 10%, 20%, 30% or by at least 40%. Such an increase of expression (herein also referred to as “overexpression”) can, e.g., be achieved by placing the coding sequence of the aldolase under the control of a heterologous promoter, in particular a heterologous promoter which ensures a higher expression of the gene. Alternatively, the endogenous promoter of the gene can be mutated so as to lead to a higher expression of the gene. Such a promoter replacement or mutation can be effected in the endogenous gene located on the chromosome of the microorganism or the corresponding modified gene with a stronger promoter can be placed on a plasmid. An example for a strong promoter which can be used to allow for the overexpression of the aldolase gene is the constitutive strong promoter pgi-20 (Braatsch et al. , Biotechniques 45 (2008), 335-337).

In addition, in order to allow for an increased expression, it can also be advantageous to use an efficient ribosome binding site. In this respect, the term “heterologous” also includes the situation in which the aldolase gene is modified so as to comprise a ribosome binding site which is different from its natural ribosomal binding site and which allows for an increased expression (translation) of the gene. An example of a suitable binding site is the ribosome binding site “C” ( AAGTT AAG AGG C AAG A) (Zelcbuch et al., Nucleic Acids Res. 41 (2013), e98).

The term “increase of expression” also covers the possibility that the activity of the enzyme is increased due to a mutation in the protein which leads to a higher activity. Means and methods for improving enzyme activity by way of mutation have been described above.

The microorganism of the present invention may be a bacterium, a fungus, such as a yeast, an alga or an archae. In one preferred embodiment, the microorganism is a bacterium. Preferred bacteria are bacteria of the genus Bacillus, Clostridium, Corynebacterium, Pseudomonas, Zymomonas or Escherichia. In a particularly preferred embodiment the bacterium belongs to the genus Escherichia and even more preferred to the species Escherichia coli. In another preferred embodiment the bacterium belongs to the species Pseudomonas putida or to the species Zymomonas mobilis or to the species Corynebacterium glutamicum or to the species Bacillus subtilis.

It is also possible to employ an extremophilic bacterium such as Thermus thermophilus, or anaerobic bacteria from the family Clostridiae.

In another preferred embodiment the microorganism is a fungus, more preferably a fungus of the genus Saccharomyces, Schizosaccharomyces, Aspergillus, Trichoderma, Kluyveromyces or Pichia and even more preferably of the species Saccharomyces cerevisiae, Schizosaccharomyces pombe, Aspergillus niger, Trichoderma reesei, Kluyveromyces marxianus, Kluyveromyces lactis, Pichia pastoris, Pichia torula or Pichia utilis.

In another embodiment, the microorganism is a photosynthetic microorganism, preferably a photosynthetic bacterium, or a microalga. In a further embodiment the microorganism is an alga, more preferably an alga belonging to the diatomeae.

In another embodiment the microorganism is a microorganism which is able to metabolize methanol. In one embodiment, the microorganism is naturally able to metabolize methanol and is, e.g. a methanothrophic bacterium, a methylotrophic bacterium, a methanothrophic yeast or a methylotrophic yeast.

In another embodiment, the microorganism is a C1 -fixing microorganism, preferably a recombinant C1 -fixing microorganism. The nature of the C1 -fixing microorganism is not particularly limited as long as it is a microorganism which is capable using carbon monoxide (CO) and gaseous substrates comprising CO like, e.g., syngas, as the source of carbon and energy. Syngas or synthesis gas is a mixture of CO and CO2 as well as H_2. Corresponding naturally occuring (or genetically modified) microorganisms are known in the art that are capable of utilizing CO and converting it into acetyl-CoA. These organisms are often referred to as acetogenic microorganisms (sometimes also termed carboxydotrophic, acetogenic microorganisms). These microorganisms use the Wood-Ljungdahl pathway to fix CO and convert it into acetyl-CoA. Examples of such microorganisms belong to the family Clostridiae and are, e.g., described in WO 2009/094485; WO 2012/05905; WO 2013/180584; US 2011/0236941; PNAS 107(29): 13087-13092 (2010); Current Opinion in Biotechnology 23:364-381 (2012); Applied and Environmental

Microbiology 77(15):5467-5475 (2011).

In certain embodiments, the C1 -fixing microorganism is selected from the group of carboxydotrophic acetogenic bacteria. In preferred embodiments, the microorganism is selected from the group comprising Clostridium autoethanogenum, Clostridium ljungdahlii, Clostridium ragsdalei, Clostridium carboxidivorans, Clostridium drakei, Clostridium scatologenes, Clostridium aceticum, Clostridium formicoaceticum, Clostridium magnum, Butyribacterium methylotrophicum, Acetobacterium woodii, Alkalibaculum bacchii, Blautia producta, Eubacterium limosum, Moorella thermoacetica, Moorella thermautotrophica, Sporomusa ovata, Sporomusa silvacetica, Sporomusa sphaeroides, Oxobacter pfennigii, and Thermoanaerobacter kiuvi.

In one embodiment, the microorganism is capable of converting methanol into formaldehyde. In a preferred embodiment microorganism is capable of providing formaldehyde by the enzymatic conversion of methanol into formaldehyde, preferably via an oxidation reaction.

One type of enzyme which can be used in this context is methanol dehydrogenase (NAD⁺) which is classified as EC 1.1.1.244. This enzyme catalyzes the reaction:

Methanol + NAD formaldehyde + NADH + H Thus, the microorganism is preferably expressing such an enzyme, either endogenously or because it has been genetically modified so as to contain a nucleic acid molecule which encodes such an enzyme. One example for such a methanol dehydrogenase is the methanol dehydrogenase encoded by the adhA gene of C. glutamicum.

Another type of enzyme which can be used for the production of formaldehyde from methanol is methanol dehydrogenase (cytochrome c; also referred to as “quinone- dependent”) which is classified as EC 1.1.2.7. Thus, the microorganism is preferably expressing such an enzyme, either endogenously or because it has been genetically modified so as to contain a nucleic acid molecule which encodes such an enzyme.

Another type of enzyme which can be used for the production of formaldehyde from methanol is a methanol oxidase, for example, methanol oxidases classified in EC 1.1.3.13. Thus, the microorganism is preferably expressing such an enzyme, either endogenously or because it has been genetically modified so as to contain a nucleic acid molecule which encodes such an enzyme.

In one embodiment, the microorganism is capable of converting methane into formaldehyde. Preferably, the microorganism is able to convert methane into methanol and to further convert methanol into formaldehyde. The conversion of methane into methanol can, e.g., be achieved by making use of a methane monooxygenase (EC 1.14.14.3 or 1.14.13.25). Thus, the microorganism is preferably expressing such (an) enzymes, either endogenously or because it has been genetically modified so as to contain (a) nucleic acid molecules which encode(s) such (an) enzyme(s).

In one embodiment, the microorganism is capable of converting halogenated methane into formaldehyde. Preferably, the microorganism is able to convert halogenated methane, e.g. dichloromethane, into formaldehyde. This conversion can, e.g., be achieved by making use of a dehalogenase, preferably a dehalogenase classified in EC 4.5.1.3). Thus, the microorganism is preferably expressing such an enzyme, either endogenously or because it has been genetically modified so as to contain a nucleic acid molecule which encodes such an enzyme.

In one embodiment, the microorganism is capable of converting methylamine (or a derivative thereof) into formaldehyde. Preferably, the microorganism is able to convert methylamine (or a derivative thereof) into formaldehyde by oxidation. This can be achieved by making, e.g., use of a methylamine dehydrogenase (classified in EC 1.4.9.1) or of a primary amine oxidase (classified in EC 1.4.3.21). Thus, the microorganism is preferably expressing such (an) enzymes, either endogenously or because it has been genetically modified so as to contain (a) nucleic acid molecules which encode(s) such (an) enzyme(s).

In one embodiment, the microorganism is capable of converting formate into formaldehyde. It has, for example, been described in Siegel et al. (Proc. Natl. Acad. Sci. USA 24 (2015), 3704-3709) that formate can be converted by acetyl-CoA synthase (ACS) into formyl-CoA which can then be further converted into formaldehyde by making use of an acetaldehyde dehydrogenase (ACDH). Thus, the microorganism is preferably expressing such an enzyme, either endogenously or because it has been genetically modified so as to contain a nucleic acid molecule which encodes such an enzyme.

In order to improve flux through the “homoserine cycle” when implemented in a microorganism, it may be advantageous to inactivate certain enzymatic activities which may endogenously occur in the respective microorganism.

Thus, in one embodiment, the microorganism is a microorganism which endogenously contains an operon encoding a formaldehyde detoxification system and in which this operon is deleted or rendered inactive. In a preferred embodiment, the microorganism belongs to the species E. coli and is a microorganism in which the formaldehyde detoxification ( frmRAB ) operon (Chen et al., Metabol. Engineering 49 (2018), 257-266) is deleted or rendered inactive. This system is also referred to as the glutathione-dependent formaldehyde oxidation system. The inactivation or deletion of this operon ensures that formaldehyde is not removed and is available for cell growth. In particular, the inactivation of this operon should avoid formaldehyde oxidation to formate which may lead to a depletion of the intracellular pool of formaldehyde.

In another embodiment, the microorganism is a microorganism which endogenously contains a gene(s) encoding (an) enzyme activity/activities which catalyze(s) the conversion of pyryvate into aspartate semialdehyde and in which this/these gene(s) is/are deleted or rendered inactive. In a preferred embodiment said gene is a gene encoding aspartate semialdehyde dehydrogenase (EC 1.2.1.11). In a preferred embodiment, the microorganism belongs to the species E. coli and is a microorganism in which the asd gene encoding said enzyme is deleted or rendered inactive. In another embodiment, the microorganism is a microorganism which endogenously contains a gene encoding a 3-phosphoglycerate dehydrogenase (EC 1.1.1.95) and in which this gene is deleted or rendered inactive. In a preferred embodiment, the microorganism belongs to the species E. coli and is a microorganism in which the serA gene encoding said enzyme is deleted or rendered inactive.

In another embodiment, the microorganism is a microorganism which endogenously contains a gene encoding a serine hydroxymethyltransferase (EC 2.1.2.1) and in which this gene is deleted or rendered inactive. In a preferred embodiment, the microorganism belongs to the species E. coli and is a microorganism in which the glyA gene encoding said enzyme is deleted or rendered inactive.

In another embodiment, the microorganism is a microorganism which endogenously contains an operon encoding a glycin cleavage system (GCS) and in which this operon is deleted or rendered inactive. In a preferred embodiment, the microorganism belongs to the species E. coli and is a microorganism in which the glycine cleavage system ( gcvTHP ) operon is deleted or rendered inactive. The GCS operon in E. coli encodes for three enzymes, i.e. GcvT, GcvH and GcvP, and it is either possible to inactive the whole operon or one or more of the three genes.

In another embodiment, the microorganism is a microorganism which endogenously contains a gene encoding a bifunctional aspartokinase/homoserine dehydrogenase (EC 1.1.1.3) and in which this gene is deleted or rendered inactive. In a preferred embodiment, the microorganism belongs to the species E. coli and is a microorganism in which the thrA gene encoding said enzyme is deleted or rendered inactive.

In another embodiment, the microorganism is a microorganism which endogenously contains a gene encoding an L-threonine 3-dehydrogenase dehydrogenase (EC 1.1.1.103) and in which this gene is deleted or rendered inactive. In a preferred embodiment, the microorganism belongs to the species E. coli and is a microorganism in which the tdh gene encoding said enzyme is deleted or rendered inactive.

In another embodiment, the microorganism is a microorganism which endogenously contains a gene encoding a 2-amino-3-ketobutyrate CoA ligase (EC 2.3.1.29) and in which this gene is deleted or rendered inactive. In a preferred embodiment, the microorganism belongs to the species E. coli and is a microorganism in which the kbl gene encoding said enzyme is deleted or rendered inactive. In another embodiment, the microorganism is a microorganism which endogenously contains a gene encoding a lactate dehydrogenase (EC 1.1.1.28) and in which this gene is deleted or rendered inactive. In a preferred embodiment, the microorganism belongs to the species E. coli and is a microorganism in which the IdhA gene encoding said enzyme is deleted or rendered inactive.

In another embodiment the microorganism shows any possible combination of gene deletions/inactivations as described above.

Figure 1 shows a schematic version of a representative example of the enzymatic steps involved in the method according to the present invention for incorporating formaldehyde.

(1) Condensation of pyruvate with formaldehyde into 4-hydroxy-2- oxobutanoic acid (HOB) via an aldolase; (2) amination of the thus produced 4-hydroxy-2-oxobutanoic acid (HOB) to produce homoserine; (3) phosphorylation of thus produced homoserine to produce o- phosphohomoserine; (4) dephosphorylation of the thus produced o- phosphohomoserine to produce threonine; (5) conversion of the thus produced threonine into glycine and acetaldehyde; (6) condensation of the thus produced glycine with formaldehyde to produce serine; and (7) conversion of the thus produced serine to produce pyruvate. Reactions based on promiscuous enzyme activities are shown in color.

Figure 2 shows in vivo “serine aldolase” (SAL) activity catalyzed by LtaE: (a) LtaE catalyzes aldol condensation between glycine and different aldehydes. Threonine aldolase (LTA) is its primary function, while serine aldolase is a promiscuous activity (Contestabile et al. , Eur. J. Biochem. 268 (2001), 6508-6525). (b), (c) two selection schemes for the in vivo activity of the SAL reaction. Carbon sources are shown in purple (glucose not shown) while the formaldehyde moiety is shown is green (b) AfrmRAB AserA AglyA strain in which methanol assimilation is required for the biosynthesis of serine (c) AfrmRAB AserA AgcvTHP strain, in which methanol assimilation is required for the biosynthesis of serine and the cellular Ci moieties (d), (e) Growth with different concentrations of methanol confirm the activity of the SAL reaction. In all cases, 10 mM glucose and 10 mM glycine were added to the medium. Methanol dehydrogenase ( mdh ) and ItaE were expressed from a plasmid. Each growth curve represents the average of three replicates, which differ from each other by less than 5%. (f), (g) Labeling pattern of proteinogenic glycine (GLY), serine (SER), threonine (THR), methionine (MET) and histidine (HIS) upon feeding with ¹³C-methanol as well as unlabeled glucose and glycine.

Figure 3 shows that Mn²⁺ supplementation improves LtaE dependent growth.

While Mn²⁺ is a cofactor of LtaE, M9 medium contains only 0.08 mM Mn²⁺. Addition of 50 mM MnCh increases the growth rate and yield of the LtaE-dependent strains. Error bars represent standard deviations, n=3.

Figure 4 Figure shows that LtaE can operate as threonine aldolase and serine aldolase simultaneously (a) Selection scheme for serine and glycine production from threonine and homoserine (b) The two selection strains, deleted in the LtaE-independent threonine cleavage system ( Akbl-tdh ), can grow with methanol as serine precursor. Glycine is either provided externally (10 mM, black lines) or produced internally, either from externally added threonine (10 mM, red lines) or from the internal pool of threonine (green lines). The latter growth confirms that LtaE can catalyze the LTA and SAL reactions simultaneously. In all cases, 10 mM glucose and 500 mM methanol were added. Each growth curve represents the average of three replicates, which differ from each other by less than 5%. (c) Labeling pattern of proteinogenic glycine and serine upon feeding with glucose labeled at different carbon as well as labeled or unlabeled methanol. This labeling confirms that all cellular serine is produced from glycine and methanol even when glycine is produced internally from threonine biosynthesis and degradation.

Figure 5 shows in vivo activity of enzymes condensing pyruvate and formaldehyde into HOB and converting HOB into homoserine, respectively (a) A selection scheme for the in vivo activity of the reactions of condensing pyruvate and formaldehyde into HOB and converting HOB into homoserine. Carbon sources are shown in purple while the formaldehyde moiety is shown is green (b) Several E. coli enzymes are known to catalyze an aldolase reaction with pyruvate as acceptor and might be able to accept formaldehyde as a donor. The sequence similarity of these enzyme is indicated by the schematic tree to the left (c) Four tested aldolases, once overexpressed together with methanol dehydrogenase, support growth of the selection strain. Glucose was added at 10 mM, methanol at 500 mM, diaminopimelate at 0.25 mM, and isoleucine at 1 mM. Each growth curve represents the average of three replicates, which differ from each other by less than 5%. (d) Labeling pattern of proteinogenic methionine (MET), threonine (THR), lysine (LYS), and aspartate (ASP) upon feeding with unlabeled glucose, diaminopimelate and isoleucine as well as ¹³C-methanol. The results confirm that all cellular threonine and methionine are derived from the enzymatic activity condensing pyruvate with formaldehyde and from the aminase reaction.

Figure 6 shows a multiple sequence alignment of candidate enzymes condensing pyruvate and formaldehyde into HOB. Protein sequence of RhmA/Yfall (P76469; SEQ ID NO:1), GarL (P23522; SEQ ID NO:4), YagE (P75682; SEQ ID NO:2), YjhH (P39359; SEQ ID NO:3), Eda (P0A955; SEQ ID NO: 45), DgoA (Q6BF16; SEQ ID NO: 46) and MhpE (P51020; SEQ ID NO: 47) were obtained from UniProt. Sequence alignment was produced by by MAFFT (Katoh and Standley, Mol. Biol. Evol. 30 (2013), 772-780. ESPpript 3.0 (Rober and Gouet, 42 (2014), W320-W324) was used for displaying the aligned sequences with the 3D structure of GarL, 1dxe (Izard and Blackwell, EMBO J. 19 (2000), 3849-3856). Protein a-helixes and b-sheets are indicated above, sequence consensus > 70% are show in cons.

Figure 7 shows protein structure based alignments of candidate enzymes condensing pyruvate and formaldehyde into HOB. Available protein structures, 1dxf (Izard and Blackwell, EMBO J. 19 (2000), 3849-3856) for GarL, 2vwt (Rea et al. , Biochemistry-US 47 (2008), 9955-9965) for RhmA, 4ptn (Manicka et al., Proteins 71 (2008), 2102-2108) for YagE, 2v82 (Walters et al., Bioorganic & Medicinal Chemistry 16 (2008), 710- 720) for DgoA and 1eua (Allard et al., Proc. Natl Acad. Sci. USA 98 (2001), 3679-3684) for Eda were obtained from RCSB PDB. Structures was aligned by TM-align (Zhang et al., Nucleic Acids Res. 33, 2302- 2309 (2005). within PyMOL. Results of the alignments, RMSD and TM- score, are shown in the table. The figures were rendered using PyMOL.

Figure 8 shows effects of genomic overexpression of the enzyme of the homoserine cycle. ThrB (HSK), ThrC (TS), AlaC* (AlaC A142P Y275D) or AspC were overexpressed by exchanging their native promoters with synthetic promoters within the selection strain AfrmRAB Aasd. Each growth curve represents the average of three replicates, which differ from each other by less than 5%.

In this specification, a number of documents including patent applications are cited. The disclosure of these documents, while not considered relevant for the patentability of this invention, is herewith incorporated by reference in its entirety. More specifically, all referenced documents are incorporated by reference to the same extent as if each individual document was specifically and individually indicated to be incorporated by reference.

The invention will now be described by reference to the following examples which are merely illustrative and are not to be construed as a limitation of the scope of the present invention.

EXAMPLES

To confirm the in vivo feasibility of the homoserine cycle, several E. coli gene deletion strains were constructed whose growth is coupled to the activity of different pathway segments. Using this approach it could be demonstrated that all required promiscuous enzymes are active enough to enable growth of the auxotrophic strains.

Methods

Strains and genomic manipulation. Strains used in this study are listed in Table 1.

Table 1

LIST OF E. COLI STRAINS

REFERENCE OR

STRAIN GENOTYPE SOURCE _ PALAC_D SIJ488 APALAC^::CAP-PPGI-2O-ALAC* THIS STUDY PAS PC D SIJ488 AP_ASPC-CAP-PPGI-2O-ASPC THIS STUDY SIJ488 AFRMRAB AASD APTHRLABC^::PPGI-2O~THRB(S THIS STUDY SIJ488 AFRMRAB AASD APMAC-PPGIGO-ALAC* THIS STUDY SIJ488 AFRMRAB AASD AP_ASP_C::PPGI-20-ASPC THIS STUDY SIJ488 AFRMRAB AASD AP HRLABC^::PPGI-2O~THRB(S THIS STUDY APALAC ^{; ;}P PGI-20-ALA C *

SIJ488 AFRMRAB AASD APTHRLABC^::PPGI-2O~TF[RBC THIS STUDY APASPC ^{; ;}P PGI-20-ASP C

¹ Jensen et al. (Sci. Rep. 5 (2015), 17874)

An E. coli MG1655 derived strain SIJ488 (Jensen et al., Scientific Reports 5 (2015), 17874) was used as the parental strain for genomic modifications. Iterative rounds of l-Red recombineering (Jensen et al., loc. cit.) or P1 phage transduction (Thomason et al., Curr. Protoc. Mol. Biol. Chapter 1, Unit 1 17 (2007)) were used for gene deletions. For the recombineering, selectable resistance cassettes were generated via PCR - primers 50 bp homologous arms as in Baba et al. (Mol. Syst. Biol. 2 (2006), 2006-2008) - using the FRT-PGK-gb2-neo-FRT (Km) cassette (Gene Bridges, Germany) for kanamycin resistance (Km) and the pKD3 plasmid (GenBank: AY048742; Datsenko and Wanner, Proc. Natl. Acad. Sci. USA 97 (2000), 6640-6645) as template for chloramphenicol resistance cassettes (CAP). The procedures of the deletion, verification and antibiotic cassette removal are detailed in Wenk et al. (Methods Enzymol. 608 (2018), 329-367).

A similar strategy was applied to exchange the genomic promoter of target genes. A constitutive strong promoter pgi-20 (Braatsch et al., Biotechniques 45 (2008), 335- 337) and a ribosome binding site “C” (AAGTTAAGAGGCAAGA (SEQ ID NO: 44); Zelcbuch et al., Nucleic Acids Res. 41 (2013), e98.) were constructed downstream of the CAP cassette using primers listed shown in Table 2.

Table 2

LIST OF OLIGO PRIMERS USED IN THIS STUDY

PRIMER SEQUENCE (5’-»3’)

RHMA_F ATGCATCATCACCATCACCACAACGCATTATTAAGCAATCCC (SEQ ID NO:6)

RHMA_R GCGCTAGCTCAATAACTACCTTTTATGC (SEQ ID NO:7)

YAGE_F ATGCATCATCACCATCACCACACCGCAGTCCGCGTTGTTC (SEQ ID NO:8)

YAGE_R GCTAGCATTAGCAAAGCTTGAGCTGTTG (SEQ ID NO:9)

YJHH_F ATGCATCATCACCATCACCACAAAAAAATTCAGCGGCATTATTCC (SEQ ID NO:10)

YJHH_R GCTAGCATTAGACTGGTAAAATGCCCT (SEQ ID NO:11)

YJHH_C TAATAAAGGGGTTGACGGGCTG (SEQ ID NO:12)

YJHH_D CAGCCCGTCAACCCCTTTATTA (SEQ ID NO:13)

YJHH_A GTAACCATTGTTGACGGGCGAG (SEQ ID NO:14)

YJHH_B CTCGCCCGTCAACAATGGTTAC (SEQ ID NO:15)

DGOA_F ATGCATCATCACCATCACCACACAGTGGCAAACTAAACTCC (SEQ ID NO:16)

DGOA_R GCTAGCATCATTGCACTGCCTCTCG (SEQ ID NO:17)

DGOA_B TCCCGCTGAACTCCCCACAATG (SEQ ID NO:18)

DGOA_C CATTGTGGGGAGTTCAGCGGGA (SEQ ID NO :19)

GAATGCATCATCACCATCACCACAAAAACTGGAAAACAAGTGCAGAATCAATCCTGACCAC

EDA_A (SEQ ID NO:20)

EDA_B GAACGGACCCGCAATCGCTTGCAGGGCTTTCAC (SEQ ID NO:21)

EDA_C GTGAAAGCCCTGCAAGCGATTGCGGGTCCGTTC (SEQ ID NO:22)

EDA_D CGCTAGCTCTAGATTACAGCTTAGCGCCTTCTACAGCTTCACG (SEQ ID NO:23)

ATGCATCATCACCATCACCACAAACGGTAAAAAACTTTATATCTCGGACG (SEQ ID

MHPE_F NO:24)

MHPE_R GCTAGCATTATTTGTTGTTGCGCAGATC (SEQ ID NO:25)

ATGCATCATCACCATCACCACATTGATTTACGCAGTGATACCGTTACCCGACC (SEQ ID

LTAE_F NO:26)

LTAE_B GCTGCGTAGTCTTGCAGAGCATTAAC (SEQ ID NO:27)

LTAE_C GTTAATGCTCTGCAAGACTACGCAGC (SEQ ID NO:28)

CTCTTACGTGCCCGATCAACGCTAGCTTAACGCGCCAGGAATGCACGCCAG (SEQ ID

LTAE_R NO :29)

GARL_F GTTAAGAGGCAAGAATGCATAATAACGATGTTTTCCCGAA (SEQ ID NO:30)

GARL_R CCGCGCTAGCTCTAGATTATTTTTTAAAGGTATCAGCCAGT (SEQ ID NO:31)

ALAC_F ATGCATCATCACCATCACCACGCTGACACTCGCCCTGAA (SEQ ID NO:32)

ALAC_C GCGCGGTGATTCCAGGGGCGCAGGTA (SEQ ID NO:33)

ALAC_B TACCTGCGCCCCTGGAATCACCGCGC (SEQ ID NO:34)

ALAC_E GCTATCACGATGACGGCACCTTTACG (SEQ ID NO:35)

ALAC_D CGTAAAGGTGCCGTCATCGTGATAGC (SEQ ID NO:36)

ALAC_R GCTAGCTTATTCCGCGTTTTCGTGAA (SEQ ID NO:37)

PALAC_R AGTAAACCGTCGGCACGGAACATC (SEQ ID NO:38)

CTCTATGATAGGTAACCTGAAGGCTGATGACCAGCAGGCCGTTTTTGAGGAATTAACCCTCACT

PALAC_F AAAGGGCG (SEQ ID NO:39)

PTHRBC_ AACCCGACGCTCATATTGGCACTGGAAGCCGGGGCATAAACTTTAACCATTCTTGCCTCTTAAC

R TTTAAAG (SEQ ID NO:40)

PTHRBC_ CAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAATTAACCCTCACT

F AAAGGGCG (SEQ ID NO:41)

GGTCCTGTTTTTTTTATACCTTCCAGAGCAATCTCACGTCTTGCAAAAACAATTAACCCTCACT

PASPC_F AAAGGGCG (SEQ ID NO:42)

GCCAGGCCCAGAATCGGGTCGGCAGGAGCGGCGGTAATGTTCTCAAACATTCTTGCCTCTTAAC PAS PC R TTTAAAG (SEQ ID NO:43) The synthetic promoter was first introduced to the SIJ488 strain by the recombineering method; P1 transduction was then used to transfer the synthetic promoter into the selection strains. thrB (encoding homoserine kinase, HSK) and thrC (encoding threonine synthase, TS) are on the same operon with thrL and thrA. Since thrL encodes a regulatory peptide and thrA is redundant in the Aasd selection strains, thrLA was deleted during the promoter exchange of thrBC. The point mutations A142P Y275D (Bouzon et al. , ACS Synthetic Biology 6 (2017), 1520-1533) were introduced along with the promoter exchange of alaC (In this case, the recombineering cassette has the mutated gene downstream the CAP cassette and synthetic promoter). Promoter exchanges were confirmed by sequencing the promoter regions.

Plasmids construction. All cloning procedures were carried out in E. coli DH5a strain. E. coli native genes ItaE, rhmA, garL, yagE, yjhH, eda, dgoA and mhpE, were cloned from E. coli MG1655 genome with the primers shown in Table 2, above.

NAD-dependent methanol dehydrogenase (CgAdhA) was taken from Corynebacterium glutamicum R after codon optimization (He et al., ACS Synthetic Biology 7 (2018), 1601-1611). Genes were inserted into a pNivC vector downstream of a ribosome binding site “C” (AAGTTAAGAGGCAAGA (SEQ ID NO: 44); Zelcbuch et al., Nucleic Acids Res. 41 (2013), e98.). The genes were assembled into one operon using BioBrick enzymes: Bcul, Sail, Nhel and Xhol (FastDigest, Thermo Scientific; Zelcbuch et al., Nucleic Acids Res. 41 (2013), e98.). Using EcoRI and Pstl, the synthetic operon was then inserted into an overexpression pZASS vector (Wenk et al., Methods Enzymol. 608 (2018), 329-367) under a constitutive strong promoter pgi-20 (Braatsch et al., Biotechniques 45 (2008), 335-337). The final plasmids are listed in the Table 3.

Table 3

SUPPLEMENTARY TABLE S4 THE LIST OF OVEREXPRESSION PLASMIDS

PLASMID _ GENES

PZASS P15A ORI; STREP^K; P_PGI-2O

PZASS-MDH PZASS v.CGADHA

PZASS-LTAE-MDH PZASS :.LTAE, CGADHA

PZASS-LTAE PZASS v.LTAE

PZASS-MDH-RHMA PZASS:: CGADHA, RHMA

PZASS-RHMA PZASS .RHMA

PZASS-MDH-GARL PZASS:: CGADHA, GARL

PZASS-GARL PZASS v.GARL

PZASS-MDH-YAGE PZASS:: CGADHA, YAGE

PZASS-YAGE PZASS:: YAGE

PZASS-MDH-YJHH PZASS:: CGADHA, YJHH

PZASS-YJHH PZASS:: YJHH

Growth media. LB medium (0.5% yeast extract, 1% tryptone, 1% NaCI) was used for strain engineering and recombinant plasmids cloning. Antibiotics were used at the following concentrations: kanamycin, 50 pg/mL; ampicillin, 100 pg/mL; streptomycin, 100 pg/mL; chloramphenicol, 30 pg/mL. Growth experiments were performed in M9 minimal media (47.8 mM Na₂HP0₄, 22 mM KH₂P0₄, 8.6 mM NaCI, 18.7 mM NH₄CI, 2 mM MgS0₄ and 100 pM CaCI₂), supplemented with trace elements (134 pM EDTA, 31 pM FeCIs, 6.2 pM ZnCI₂, 0.76 pM CuCI₂, 0.42 pM CoCI₂, 1.62 pM H₃B0₃, 0.081 pM MnCI₂). Additional 50 pM MnCI₂ was added for all experiments since it improves in vivo activity of LtaE (Figure 3). Carbon sources were added according to the strain and the specific experiment: 10 mM glucose, 10 mM glycine, 10 mM serine, 10 mM threonine, 2 mM homoserine, and 1 mM isoleucine. 0.25 mM diaminopimelate (DAP) was supplemented in all media used to cultivate the Aasd strain (Cardineau and Curtiss, J. Biol. Chem. 262 (1987), 3344-3353).

Growth experiments. Strains were precultured in 4 mL M9 medium with proper carbon sources and streptomycin. The precultures were harvested and washed three times in M9 medium, then inoculated in M9 media with suitable carbon sources, with a starting OD₆oo of 0.02. 150 pL of culture were added to each well of 96-well microplates (Nunclon Delta Surface, Thermo Scientific). Further 50 pL mineral oil (Sigma-Aldrich) was added to each well to avoid evaporation (while enabling gas diffusion). The 96-well microplates were incubated at 37°C in microplate reader (BioTek EPOCH 2). The shaking program cycle (controlled by Gen5 v3) had 4 shaking phases, lasting 60 seconds each: linear shaking followed by orbital shaking, both at an amplitude of 3 mm, then linear shaking followed by orbital shaking both at an amplitude of 2 mm. The absorbance (OD₆oo) in each well was monitored and recorded after every three shaking cycles (~16.5 min). Raw data from the plate reader were calibrated to normal cuvette measured OD₆oo values according to OD_Cuvette=ODpiate/0.23. Growth parameters were calculated using MATLAB (MathWorks) based on three technical triplicates - the average values were used to generate the growth curves. Checked in MATLAB, in all cases variability between triplicate measurements were less than 5%.

Stable isotopic labelling. ¹³C-Methanol, glucose-1 -¹³C, glucose-2-¹³C, glucose-3- 1³C were purchased from Sigma-Aldrich. Cells were harvested at the late exponential phase. The equivalent volume of 1 mL of culture at OD₆oo of 1 was harvested and washed by centrifugation. Protein biomass was hydrolyzed with 6 M HCI, at 95°C for 24 h (You et al., Journal of Visualized Experiments 59 (2012)). The samples were completely dried under a stream of air at 95°C. Hydrolyzed amino acids were analyzed with UPLC-ESI-MS as previously described (Giavalisco et al., Plant J. 68 (2011 ), 364-376). Chromatography was performed with a Waters Acquity UPLC system (Waters), using an HSS T3 Cis reversed phase column (100 mm ^c 2.1 mm, 1.8 pm; Waters). 0.1% formic acid in H₂0 (A) and 0.1 % formic acid in acetonitrile (B) were the mobile phases. The flow rate was 0.4 mL/min and the gradient was: 0 to 1 min - 99% A; 1 to 5 min - linear gradient from 99% A to 82%; 5 to 6 min - linear gradient from 82% A to 1 % A; 6 to 8 min - kept at 1 % A; 8-8.5 min - linear gradient to 99% A; 8.5-11 min - re-equilibrate. Mass spectra were acquired using an Exactive mass spectrometer (Thermo Scientific) in positive ionization mode, with a scan range of 50.0 to 300.0 m/z. The spectra were recorded during the first 5 min of the LC gradients. Data analysis was performed using Xcalibur (Thermo Scientific). The identification amino acids was based on retention times and m/z, which were determined by analyzing amino acid standards (Sigma-Aldrich) under the same conditions.

Molecular phylogenetic analysis. The protein sequences of the aldolases used to catalyze the HAL reaction were obtained from UniProt: RhmA/YfaU P76469, GarL P23522, YagE P75682 and YjhH P39359, Eda P0A955, DgoA Q6BF16 and MhpE P51020. MAFFT v7 (Katoh and Standley, Mol. Biol. Evol. 30 (2013), 772-780) was used for multiple sequence alignment with default parameters. The aligned sequences were used by MEGA X (Kumar et al., Mol. Biol. Evol. 35 (2018), 1547- 1549) with Maximum Likelihood method to construct a phylogenetic tree. The bootstrap consensus tree was generated with the setting No. of bootstrap replications to 1000.

Example 1

Concept of the newly developed “homoserine cycle’

With the aim to provide a metabolic route which is superior to the known native serine cycle, a metabolic pathway was designed which is also referred herein as the “homoserine cycle”. A representative example of the homoserine cycle is shown in Figure 1 .

In the homoserine cycle, glycine is directly condensed with formaldehyde to generate serine. This reaction (item (6) in Fig. 1 ) (herein also referred to as the serine aldolase (SAL) reaction) was previously found to be promiscuously catalyzed {in vitro) by a threonine aldolase (LtaE) (Contestabile et al. , Eur. J. Biochem. 268 (2001 ), 6508- 6525). The SAL reaction bypasses the very long, multi-cofactor-dependent, and ATP- inefficient route for formaldehyde assimilation to 5,10-methylene-tetrahydrofolate (CH₂-THF) (Crowther et al. , J. Bacteriol. 190 (2008), 5057-5062). As within the previously proposed modified serine cycles (Yu and Liao, Nature Communications 9 (2018), 3992; Bar-Even, Biochemistry 55 (2016), 3851-3863), serine is then deaminated to pyruvate by serine deaminase (item (7) in Fig. 1), bypassing a longer route via glycerate, which further involves the highly toxic intermediate hydroxy pyruvate (Kim and Copley, Proc. Natl. Acad. Sci. USA 109 (2012), E2856- 2864). Pyruvate is then condensed with formaldehyde to generate the non-natural metabolite 4-hydroxy-2-oxobutanoate (HOB) (Bouzon et al., ACS Synthetic Biology 6 (2017), 1520-1533), which is subsequently aminated to homoserine. The first of these reactions - (item (1 ) in Fig. 1 ) - was found to be promiscuously catalyzed by E. coli 2-keto-3-deoxy-L-rhamnonate aldolase (RhmA) (Hernandez et al., ACS Catal. 7 (2017), 1707-1711). The latter reaction - HOB amination (item (2) in Fig. 1) - is supported by numerous aminotransferases (Hernandez et al., ACS Catal. 7 (2017), 1707-1711; Walther et al., Metab. Eng. 45 (2018), 237-245; Zhong et al., ACS Synthetic Biology 8 (2019), 587-595) as well as amino acid dehydrogenases such as (engineered) glutamate dehydrogenase (Chen et al., Biotechnol. J. 10 (2015), 284- 289). This route effectively replaces a carboxylation reaction (by phosphoenolpyruvate carboxylase) with a formaldehyde assimilation reaction that provides an alternative way to generate a C₄ intermediate. Homoserine is then converted into threonine, e.g. by the action of homoserine kinase (ThrB, item (3) in Fig. 1) and threonine synthase (ThrC, item (4) in Fig. 1). Finally, threonine is cleaved to produce glycine and acetaldehyde. This can, e.g., be achieved by making use of a threonine aldolase (item (5) in Fig. 1, for example by the same threonine aldolase (LtaE) that catalyzes the SAL reaction (item (6) in Figure 1) to regenerate glycine and produce acetaldehyde. The produced acetaldehyde can, e.g., be further oxidized to acetyl-CoA and assimilated to central metabolism.

Demonstration of the in vivo activity of enzymes catalyzing the condensation of qlvcine and formaldehyde into serine

As described above, several of the reactions of the newly proposed “homoserine cycle” correspond to the primary activities of their catalyzing enzymes and, thus, are expected to be normally catalyzed in vivo by the respective enzymes and it is also expected that these reactions do not constrain pathway flux. However, three of the reactions of the newly designed “homoserine cycle” (i.e. items (1), (2) and (6) in Figure 1), correspond to promiscuous activities that had so far only been characterized in vitro. Hence, for each of these promiscuous activities, it was tested whether they can also support the corresponding conversions in vivo. For this purpose, dedicated gene deletion strains were created, the growth of which is dependent on the activity of these reactions.

First, the ability of LtaE to catalyze the “SAL reaction” (item (6) in Figure 1 ; Figure 2a) in vivo was tested. Towards this aim two strains auxotrophic for glycine and serine were constructed. In both strains the gene encoding for 3-phosphoglycerate dehydrogenase ( AserA ) was deleted. In one strain the gene encoding serine hydroxymethyltransferase was also deleted ( AglyA ) while in the other strain the genes for the glycine cleavage system was deleted ( AgcvTHP ). The growth of these strains required the addition of both glycine and serine, as the cellular interconversion of these compounds is blocked (Fig. 2b and c).

It was reasoned that if the SAL reaction indeed supports physiologically relevant flux, both strains should be able to grow when methanol dehydrogenase (MDH) and LtaE are overexpressed and serine is replaced with methanol in the medium. In the AserA AglyA strain, the SAL reaction would be responsible for the production of serine (Fig. 2b), which accounts for ~3% of the carbon in biomass (Neidhardt et al. , in: Physiology of the Bacterial Cell: A Molecular Approach 134-143 (1990)). In the AserA AgcvTHP strain, the SAL reaction would be responsible for the production of both serine and the cellular Ci moieties (Fig. 2c), together accounting for ~6% of the carbon in biomass (Neidhardt et al., loc. cit.). To avoid formaldehyde oxidation to formate, which might deplete its intracellular pool and constrain its assimilation, the genes encoding for the glutathione-dependent formaldehyde oxidation system were also deleted ( AfrmRAB ) (He et al. , ACS Synthetic Biology 7 (2018), 1601 -1611 ).

Upon overexpression of MDH and LtaE growth of both selection strains was observed with glucose as the main carbon source and glycine and methanol as precursors of serine (Fig. 2d,e). This indicates that the SAL reaction can operate in vivo at a physiologically significant rate. Expression of only MDH or only LtaE failed to sustain growth, indicating that the native expression of genomic ItaE is too low to support the SAL reaction. The observed growth rate and yield were dependent on the concentration of methanol, where the AserA AglyA AfrmRAB strain supported higher rates and yields than the AserA AgcvTHP AfrmRAB strain on low methanol concentrations. This corresponds to the prediction that the latter strain depends on the SAL reaction to provide a higher fraction of the cellular carbons. Methanol concentrations in the range of 200-1000 mM seem to be optimal, supporting growth rates similar to that of the positive control (in which serine is added to the medium). In all experiments, 50 mM MnCh were added, as Mn²⁺ is a known cofactor of LtaE (Fesko, Appl. Microbiol. Biotechnol. 100 (2016), 2579-2590). Without the additional supplementation of MnCh, we the reactions still occur, but lower growth rates and yields were observed (see Figure 3).

To confirm the activity of the SAL reaction, ¹³C-labeling experiments were conducted. Both strains were cultivated with ¹³C-methanol as well as unlabeled glucose and glycine. In the AserA AglyA AfrmRAB strain serine was found to be entirely singly labeled as expected, while the other amino acids were unlabeled (Fig. 2f). As threonine and methionine are derived from oxaloacetate, and hence carry a carbon that originates from CO2 (i.e. , anaplerotic reactions), their lack of labeling indicates that formaldehyde oxidation to CO2 is negligible, as expected by the deletion of frmRAB. In the AserA AgcvTHP AfrmRAB strain serine, methionine, and histidine were found to be entirely singly labeled (Fig. 2g). Unlike threonine, both methionine and histidine harbor a carbon derived from THF carrying a Ci unit - methyl-THF in the case of methionine and formyl-THF in the case of histidine (Yishai et al. , ACS Synthetic Biology 6(9) (2017), 1722-1731). The labeling of these amino acids thus indicates that all cellular Ci moieties are derived from methanol. Overall, the labeling results confirm that the SAL reaction provides the sole source of serine in both strains and the sole source of Ci moieties in the AserA AgcvTHP AfrmRAB strain.

Next, it was tested whether it is possible to omit glycine from the medium, such that it will be produced endogenously via LtaE-dependent threonine cleavage (Fig. 4a). Towards this aim, in both selection strains, the genes encoding for threonine dehydrogenase and 2-amino-3-ketobutyrate CoA-ligase (Akb!-tdh) were deleted, thus blocking the LtaE-independent route of threonine degradation (Fig. 4a) (Yishai et al., ACS Synthetic Biology 6(9) (2017), 1722-1731). It was found that replacing glycine with threonine did not alter the growth of either selection strain (Fig. 4b, note that growth was still strictly dependent on methanol). To enable this growth, the overexpressed LtaE catalyzes two subsequent reactions, first cleaving threonine to glycine and acetaldehyde and then reacting glycine with formaldehyde to produce serine. Hence, LtaE can be regarded as a glycyltransferase - transferring a glycine moiety from one small aldehyde (acetaldehyde) to another (formaldehyde).

Next, it was assessed whether it was also possible to omit threonine from the medium and rely on native threonine biosynthesis to provide this amino acid as a precursor for glycine and serine (Fig. 4a). Indeed, despite showing reduced growth rates, both selection strains were able to grow with only glucose (as main carbon source) and methanol without the addition of glycine or threonine (green lines in Fig. 4b). This indicates that half of the homoserine cycle is active: homoserine (generated natively from aspartate) is metabolized to threonine via homoserine kinase (HSK) and threonine synthase (TS), and LtaE then cleaves threonine to glycine (and acetaldehyde) and condenses glycine with formaldehyde to produce serine (Fig. 4a). To confirm that, also in the absence of externally provided glycine or threonine, all cellular serine is produced from glycine condensation with formaldehyde, ¹³C-labeling experiments were conducted. The strains were cultured in the presence of labeled or unlabeled methanol as well as glucose labeled at different carbons (glucose-1 -¹³C, glucose-2-¹³C, and glucose-3-¹³C). While the labeling pattern of glycine changed according to the labeled carbon of glucose, cultivation with ¹³C-methanol always resulted in exactly one more labeled carbon in serine than in glycine (Fig. 4c). This unequivocally confirms the methanol-dependent production of serine from glycine when the latter compound is produced internally from homoserine metabolism. Overall, the obtained results confirm the capability of LtaE to convert threonine to serine in vivo by releasing acetaldehyde and assimilating formaldehyde. The findings further confirm the physiologically significant activity of half of the homoserine cycle, where homoserine metabolism to glycine and serine provided all the biomass requirement of these amino acids as well as cellular Ci moieties, together consisting 10% of the carbon in biomass (Neidhardt et al. , in: Physiology of the Bacterial Cell: A Molecular Approach 134-143 (1990)).

Demonstration of the in vivo activity of enzymes catalyzing the condensation of qlvcine and pyruvate to form HOB and of the conversion of HOB into homoserine

After demonstrating methanol-dependent conversion of homoserine to serine, the aim was to demonstrate methanol-dependent conversion of pyruvate to homoserine. To select for the in vivo conversion of pyruvate to homoserine and threonine via HOB production and amination, a homoserine auxotroph strain was constructed: a deletion of the gene coding for aspartate-semialdehyde dehydrogenase ( Aasd ) resulted in a strain capable of growing only when homoserine and diaminopimelate (DAP) (Cardineau and Curtiss, J. Biol. Chem. 262 (1987), 3344-3353) were added to the medium. In this strain, homoserine is metabolized to methionine, threonine, and isoleucine, while DAP is metabolized to lysine and peptidoglycans. (It is noted that despite being formally reversible, homoserine dehydrogenase was not able to oxidize homoserine to aspartate-semialdehyde, the precursor of DAP, and hence the addition of the latter intermediate to the medium was required).

It was reasoned that, in the presence of methanol and methanol dehydrogenase, the combined activities of an enzyme catalyzing the formation of HOB by condensing pyruvate with formaldehyde and of an enzyme catalyzing the conversion of HOB into homoserine should enable the Aasd AfrmRAB strain to grow without the addition of homoserine to the medium (Fig. 5a). So far only RhmA was previously shown to catalyze the condensation of puryvate with formaldehyde to produce HOB (Hernandez et al. , ACS Catal. 7 (2017), 1707-1711). In order to find other enzymes which can catalyze this reaction, similar aldolases were tested for this activity. Hence, a search was conducted for all E. coli enzymes (strain MG1655, using EcoCyc; Keseler et al., Nucleic Acids Res. 33 (2005), D334-337) that are known to catalyze an aldolase reaction with pyruvate as an acceptor (and which might be able to use formaldehyde as a donor). Besides RhmA itself, six candidate aldolases were found: GarL, YagE, YjhH, Eda, DgoA, and MhpE (Fig. 5b). RhmA and GarL belong to the structural family of HpcH while mhpE belongs to DmpG family. Both of these families are Type II pyruvate aldolases which use a divalent metal cation for donor binding and enolization (Fang et al., Angew. Chem. Int. Ed. Engl. 58 (2019), 11841-11845). YagE and YjhH belong to the structural family of DHDPS while Eda and DgoA belong to KDPG family. These families are Type I pyruvate aldolases, using a lysine residue to form a Schiff base with the donor substrate (Fang et al., loc cit. ).

It was found that overexpression of mdh together with rhmA, garL, yagE, or yjhH enabled growth of the Aasd AfrmRAB strain when homoserine was replaced with methanol (Fig. 5c). These four aldolase enzymes supported roughly the same growth rates. No growth was observed without methanol, or when methanol dehydrogenase or the aldolase enzymes were overexpressed alone. The relative sequence similarity between RhmA, GarL, YagE, and YjhH (Fig. 5b and Fig. 6 might explain why these enzymes, and not the others, were able to support the HAL reaction. Indeed, the structure of RhmA and GarL is almost identical (Figure 7).

As the reaction converting HOB into homoserine is known to be supported by the native aspartate aminotransferase (AspC), a highly expressed protein (Li et al., Cell 157 (2014), 624-635), and might be further catalyzed by other highly expressed, promiscuous aminotransferases, it was hypothesized that no dedicated enzyme overexpression would be required to enable this key reaction and this was indeed the case. In particular, growth was possible without dedicated overexpression of an aminotransferase enzyme. Genomic overexpression of aspC or of a mutated version of alanine aminotransferase (a/aC*, the protein product of which was previously shown to catalyze the reaction in which homoserine is formed from HOB; Bouzon et al., ACS Synthetic Biology 6 (2017), 1520-1533) did not alter growth substantially (Figure 8). Similarly, genomic overexpression of thrBC did not consistently assist growth (Figure 8). This indicates that the reaction leading from HOB to homoserine, the homoserine kinase (HSK) reaction and the threonine synthase (TS) reaction do not constrain the flux from pyruvate to threonine.

To confirm that homoserine, and its downstream products threonine and methionine are produced from pyruvate and methanol via the reactions in which HOB is formed by the condensation of pyruvate with formaldehyde and the subsequent conversion of HOB into homoserine, ¹³C-labeling experiments we performed. Upon cultivation with unlabeled glucose and ¹³C-methanol, threonine and methionine were found to be completely once labeled, where lysine and aspartate (serving as control) were fully unlabeled. This confirms that homoserine and threonine are completely derived from pyruvate and methanol.

Claims

1. A method comprising the following enzymatically catalyzed steps

(1) condensation of pyruvate with formaldehyde into 4-hydroxy-2-oxobutanoic acid (HOB);

(2) amination of the thus produced 4-hydroxy-2-oxobutanoic acid (HOB) to produce homoserine;

(3) conversion of thus produced homoserine to threonine;

(4) conversion of the thus produced threonine into glycine and acetaldehyde or acetyl-CoA;

(5) condensation of the thus produced glycine with formaldehyde to produce serine; and

(6) conversion of the thus produced serine to produce pyruvate, wherein said pyruvate can then be used as a substrate in step (1 ).

2. The method of claim 1 , wherein the conversion of homoserine to threonine in step (3) takes place by

(iii) phosphorylation of thus produced homoserine to produce o- phosphohomoserine; and

(iv) dephosphorylation of the thus produced o-phosphohomoserine to produce threonine.

3. The method of claim 1 or 2, wherein the conversion of serine into pyruvate in step (6) is achieved by deamination.

4. The method of any one of claims 1 to 3, wherein

(a) in step (1 ) the condensation of pyruvate with formaldehyde into 4-hydroxy- 2-oxobutanoic acid (HOB) is achieved using an aldolase classified in EC 4.1.2._;

(b) in step (2) the amination of the thus produced 4-hydroxy-2-oxobutanoic acid (HOB) to produce homoserine is achieved by using an aminotransferase enzyme classified in EC 2.6.1._ or by an amino acid dehydrogenase (EC 1.4.1._); (c) in step (3)(i) of claim 2 the phosphorylation of thus produced homoserine to produce o-phosphohomoserine is achieved by using a homoserine kinase (EC 2.7.1.39);

(d) in step (3) (ii) of claim 2 the dephosphorylation of the thus produced o- phosphohomoserine to produce threonine is achieved by using a threonine synthase (EC 4.2.3.1);

(e) in step (4) the conversion of the thus produced threonine into glycine and acetaldehyde is achieved by using a threonine aldolase (selected from the group consisting of EC 4.1.2.5, EC 4.1.2.6, EC 4.1.2.48 and EC 4.1.2.49) and/ or wherein in step (5) the conversion of the thus produced threonine into glycine and acetyl-CoA is achieved by a combination of a threonine dehydrogenase (EC 1.1.1.103) and a 2-amino-3-ketobutyrate CoA ligase (EC 2.3.1.29);

(f) in step (5) the condensation of the thus produced glycine with formaldehyde to produce serine is achieved by using a threonine aldolase (selected from the group consisting of EC 4.1.2.5, EC 4.1.2.6, EC 4.1.2.48 and EC 4.1.2.49);

(g) in step (6) the conversion of the thus produced serine to produce pyruvate is achieved by using a serine deaminase (EC 4.3.1.17) or a threonine deaminase (EC4.3.1.19); or any combination of (a) to (g).

5. The method of any one of claims 1 to 4, wherein the formaldehyde used for the condensation in step (1) and/or (5) is provided by the oxidation of methanol.

6. The method of any one of claims 1 to 5, wherein the formaldehyde in step (1) and/or 5) is provided by

(a) converting methanol enzymatically into formaldehyde using a methanol dehydrogenase (EC 1.1.1.244) or a methanol dehydrogenase (cytochrome c) (EC 1.1.2.7); and/or

(b) converting methanol enzymatically into formaldehyde using an alcohol oxidase (EC 1.1.3.13).

7. A recombinant microorganism expressing enzymes for catalyzing the following reactions:

(1) condensation of pyruvate with formaldehyde into 4-hydroxy-2-oxobutanoic acid (HOB) by an aldolase classified in EC 4.1.2._; (2) amination of the thus produced 4-hydroxy-2-oxobutanoic acid (HOB) to produce homoserine by an aminotransferase enzyme classified in EC 2.6.1 or by an amino acid dehydrogenase (EC 1.4.1._);

(3) phosphorylation of thus produced homoserine to produce o- phosphohomoserine by a homoserine kinase (EC 2.7.1.39);

(4) dephosphorylation of the thus produced o-phosphohomoserine to produce threonine by a threonine synthase (EC 4.2.3.1);

(5) conversion of the thus produced threonine into glycine by a threonine aldolase (selected from the group consisting of EC 4.1.2.5, EC 4.1.2.6, EC 4.1.2.48 and EC 4.1.2.49) or by a combination of a threonine dehydrogenase (EC 1.1.1.103) and a 2-amino-3-ketybutyrate CoA ligase (EC 2.3.1.29);

(6) condensation of the thus produced glycine with formaldehyde to produce serine by a threonine aldolase (selected from the group consisting of EC 4.1.2.5, EC 4.1.2.6, EC 4.1.2.48 and EC 4.1.2.49); and

(7) deamination of the thus produced serine to produce pyruvate by a serine deaminase (EC 4.3.1.17 or a threonine deaminase (EC 4.3.1.19)), wherein said pyruvate can then be used as a substrate in step (1), wherein said microorganism contains at least one heterologous nucleic acid molecule encoding the aldolase catalyzing step (1) and overexpresses the enzyme catalyzing step (6), i.e. the condensation of glycine with formaldehyde to form serine.

8. The microorganism of claim 7 which furthermore overexpresses at least one of the enzymes catalyzing step (3), step (4) or step (5).

9. The microorganism of claim 7 or 8 which is capable of converting methanol into formaldehyde.

10. The microorganism of claim 9, wherein said microorganism

(a) converts methanol enzymatically into formaldehyde by a methanol dehydrogenase (EC 1.1.1.244) or a methanol dehydrogenase (cytochrome c) (EC 1.1.2.7); and/or

(b) converts methanol enzymatically into formaldehyde by an alcohol oxidase (EC 1.1.3.13).

11. The microorganism of any one of 7 to 10, wherein said microorganism is deficient in an enzyme activity converting pyryvate into aspartate semialdehyde.

12. The microorganism of any one of 7 to 11, wherein said microorganism is deficient in the enzyme activity of 3-phosphoglycerate dehydrogenase (EC 1.1.1.95).

13. The microorganism of any one of 7 to 12, wherein said microorganism is deficient in the enzyme activity of serine hydroxymethyltransferase (EC 2.1.2.1) and/or in the glycine cleavage system (gcvTHP).

14. The microorganism of any one of claims 7 to 13 which is E. coli.