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EP2971024A4 - Thioesterases and cells for production of tailored oils - Google Patents

Thioesterases and cells for production of tailored oils

Info

Publication number
EP2971024A4
EP2971024A4 EP14769502.7A EP14769502A EP2971024A4 EP 2971024 A4 EP2971024 A4 EP 2971024A4 EP 14769502 A EP14769502 A EP 14769502A EP 2971024 A4 EP2971024 A4 EP 2971024A4
Authority
EP
European Patent Office
Prior art keywords
thioesterases
cells
production
tailored oils
tailored
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP14769502.7A
Other languages
German (de)
French (fr)
Other versions
EP2971024A1 (en
Inventor
George N Rudenko
Jason Casolari
Scott Franklin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Corbion Biotech Inc
Original Assignee
TerraVia Holdings Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US13/837,996 external-priority patent/US9290749B2/en
Application filed by TerraVia Holdings Inc filed Critical TerraVia Holdings Inc
Publication of EP2971024A1 publication Critical patent/EP2971024A1/en
Publication of EP2971024A4 publication Critical patent/EP2971024A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/64Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409Fatty acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y301/00Hydrolases acting on ester bonds (3.1)
    • C12Y301/02Thioester hydrolases (3.1.2)
    • C12Y301/02014Oleoyl-[acyl-carrier-protein] hydrolase (3.1.2.14), i.e. ACP-thioesterase
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Edible Oils And Fats (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Fats And Perfumes (AREA)
EP14769502.7A 2013-03-15 2014-03-13 Thioesterases and cells for production of tailored oils Withdrawn EP2971024A4 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201361791861P 2013-03-15 2013-03-15
US13/837,996 US9290749B2 (en) 2013-03-15 2013-03-15 Thioesterases and cells for production of tailored oils
US201361917217P 2013-12-17 2013-12-17
PCT/US2014/026644 WO2014151904A1 (en) 2013-03-15 2014-03-13 Thioesterases and cells for production of tailored oils

Publications (2)

Publication Number Publication Date
EP2971024A1 EP2971024A1 (en) 2016-01-20
EP2971024A4 true EP2971024A4 (en) 2016-11-16

Family

ID=51581068

Family Applications (1)

Application Number Title Priority Date Filing Date
EP14769502.7A Withdrawn EP2971024A4 (en) 2013-03-15 2014-03-13 Thioesterases and cells for production of tailored oils

Country Status (9)

Country Link
EP (1) EP2971024A4 (en)
JP (1) JP2016518112A (en)
KR (1) KR20150128770A (en)
CN (1) CN105143458A (en)
AU (2) AU2014236763B2 (en)
BR (1) BR112015023192A8 (en)
CA (1) CA2904395A1 (en)
MX (1) MX2015011507A (en)
WO (1) WO2014151904A1 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9567615B2 (en) 2013-01-29 2017-02-14 Terravia Holdings, Inc. Variant thioesterases and methods of use
US9816079B2 (en) 2013-01-29 2017-11-14 Terravia Holdings, Inc. Variant thioesterases and methods of use
US9783836B2 (en) 2013-03-15 2017-10-10 Terravia Holdings, Inc. Thioesterases and cells for production of tailored oils
US9290749B2 (en) 2013-03-15 2016-03-22 Solazyme, Inc. Thioesterases and cells for production of tailored oils
EP3660149A1 (en) * 2014-07-24 2020-06-03 Corbion Biotech, Inc. Variant thioesterases and methods of use
EP3194582A2 (en) * 2014-09-18 2017-07-26 TerraVia Holdings, Inc. Acyl-acp thioesterases and mutants thereof
US20180142218A1 (en) 2016-10-05 2018-05-24 Terravia Holdings, Inc. Novel acyltransferases, variant thioesterases, and uses thereof
US20230143841A1 (en) 2020-01-16 2023-05-11 Corbion Biotech, Inc. Beta-ketoacyl-acp synthase iv variants

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992020236A1 (en) * 1991-05-21 1992-11-26 Calgene, Inc. Plant medium-chain thioesterases
CN102586350A (en) * 2012-01-09 2012-07-18 北京化工大学 Production method for C8:0/C10:0/C12:0/C14:0 medium-chain fatty acid and ethyl ester thereof

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5344771A (en) 1990-04-26 1994-09-06 Calgene, Inc. Plant thiosterases
US5298421A (en) 1990-04-26 1994-03-29 Calgene, Inc. Plant medium-chain-preferring acyl-ACP thioesterases and related methods
US5639790A (en) 1991-05-21 1997-06-17 Calgene, Inc. Plant medium-chain thioesterases
US5455167A (en) * 1991-05-21 1995-10-03 Calgene Inc. Medium-chain thioesterases in plants
US5850022A (en) 1992-10-30 1998-12-15 Calgene, Inc. Production of myristate in plant cells
US5654495A (en) 1992-10-30 1997-08-05 Calgene, Inc. Production of myristate in plant cells
US5910631A (en) * 1993-09-03 1999-06-08 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Middle chain-specific thioesterase genes from Cuphea lanceolata
EP0728212A1 (en) 1993-11-10 1996-08-28 Calgene, Inc. Plant acyl acp thioesterase sequences
US5807893A (en) 1993-11-18 1998-09-15 Voelker; Toni Alois Plant thioesterases and use for modification of fatty acid composition in plant seed oils
CN101765661B (en) 2007-06-01 2014-08-06 索拉兹米公司 Production of oil in microorganisms
ES2545083T3 (en) * 2007-07-09 2015-09-08 Bayer Cropscience Nv Brassica plant comprising mutant alleles of acyl fatty-ACP thioesterase
US7982035B2 (en) 2007-08-27 2011-07-19 Duquesne University Of The Holy Spirit Tricyclic compounds having antimitotic and/or antitumor activity and methods of use thereof
AU2009259433A1 (en) * 2008-06-20 2009-12-23 Basf Plant Science Gmbh Plants having enhanced yield-related traits and a method for making the same
US7883882B2 (en) 2008-11-28 2011-02-08 Solazyme, Inc. Renewable chemical production from novel fatty acid feedstocks
CA2758301A1 (en) * 2009-04-10 2010-10-14 Ls9, Inc. Production of fatty acid derivatives
AU2011257983B2 (en) 2010-05-28 2016-02-11 Corbion Biotech, Inc. Food compositions comprising tailored oils
CN103827307A (en) 2011-02-02 2014-05-28 索拉兹米公司 Tailored oils produced from recombinant oleaginous microorganisms
US8951762B2 (en) * 2011-07-27 2015-02-10 Iowa State University Research Foundation, Inc. Materials and methods for using an acyl—acyl carrier protein thioesterase and mutants and chimeras thereof in fatty acid synthesis
ES2744868T3 (en) 2012-04-18 2020-02-26 Corbion Biotech Inc Custom-made oils

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992020236A1 (en) * 1991-05-21 1992-11-26 Calgene, Inc. Plant medium-chain thioesterases
CN102586350A (en) * 2012-01-09 2012-07-18 北京化工大学 Production method for C8:0/C10:0/C12:0/C14:0 medium-chain fatty acid and ethyl ester thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
See also references of WO2014151904A1 *
YUAN L ET AL: "MODIFICATION OF THE SUBSTRATE SPECIFICITY OF AN ACYL-ACYL CARRIER PROTEIN THIOESTERASE BY PROTEIN ENGINEERING", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, US, vol. 92, 1 November 1995 (1995-11-01), pages 10639 - 10643, XP002014019, ISSN: 0027-8424, DOI: 10.1073/PNAS.92.23.10639 *

Also Published As

Publication number Publication date
CA2904395A1 (en) 2014-09-25
WO2014151904A1 (en) 2014-09-25
AU2014236763B2 (en) 2018-08-23
AU2018267601A1 (en) 2018-12-06
MX2015011507A (en) 2016-04-07
BR112015023192A2 (en) 2017-11-21
BR112015023192A8 (en) 2018-01-02
JP2016518112A (en) 2016-06-23
EP2971024A1 (en) 2016-01-20
AU2014236763A1 (en) 2015-10-01
KR20150128770A (en) 2015-11-18
CN105143458A (en) 2015-12-09

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Legal Events

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PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

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17P Request for examination filed

Effective date: 20150921

AK Designated contracting states

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Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

AX Request for extension of the european patent

Extension state: BA ME

DAX Request for extension of the european patent (deleted)
RIC1 Information provided on ipc code assigned before grant

Ipc: C12N 9/00 20060101ALI20160630BHEP

Ipc: C12N 5/02 20060101ALI20160630BHEP

Ipc: C12P 7/64 20060101AFI20160630BHEP

Ipc: C12N 5/00 20060101ALI20160630BHEP

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: TERRAVIA HOLDINGS, INC.

A4 Supplementary search report drawn up and despatched

Effective date: 20161013

RIC1 Information provided on ipc code assigned before grant

Ipc: C12N 5/00 20060101ALI20161007BHEP

Ipc: C12P 7/64 20060101AFI20161007BHEP

Ipc: C12N 5/02 20060101ALI20161007BHEP

Ipc: C12N 9/00 20060101ALI20161007BHEP

17Q First examination report despatched

Effective date: 20170711

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: CORBION BIOTECH, INC.

RIN1 Information on inventor provided before grant (corrected)

Inventor name: CASOLARI, JASON

Inventor name: FRANKLIN, SCOTT

Inventor name: RUDENKO, GEORGE N.

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20200603