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EP2284270A3 - Procédé d'identification et d'isolation des polypeptides de liaison à partir des bibliothèques combinatoires de protéines dotées d'une structure d'échafaudage des domaines par exemple de la lectine de type C - Google Patents

Procédé d'identification et d'isolation des polypeptides de liaison à partir des bibliothèques combinatoires de protéines dotées d'une structure d'échafaudage des domaines par exemple de la lectine de type C Download PDF

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Publication number
EP2284270A3
EP2284270A3 EP10185435A EP10185435A EP2284270A3 EP 2284270 A3 EP2284270 A3 EP 2284270A3 EP 10185435 A EP10185435 A EP 10185435A EP 10185435 A EP10185435 A EP 10185435A EP 2284270 A3 EP2284270 A3 EP 2284270A3
Authority
EP
European Patent Office
Prior art keywords
ctlds
libraries
tetranectin
domains
type lectin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP10185435A
Other languages
German (de)
English (en)
Other versions
EP2284270A2 (fr
Inventor
Michael Etzerodt
Thor Las Holtet
Niels Jonas Heilskov Graversen
Hans Christian Thøgersen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bird Rock Bio Inc
Original Assignee
Anaphore Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Anaphore Inc filed Critical Anaphore Inc
Priority claimed from EP01270552A external-priority patent/EP1341912B1/fr
Publication of EP2284270A2 publication Critical patent/EP2284270A2/fr
Publication of EP2284270A3 publication Critical patent/EP2284270A3/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1044—Preparation or screening of libraries displayed on scaffold proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4726—Lectins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP10185435A 2000-12-13 2001-12-13 Procédé d'identification et d'isolation des polypeptides de liaison à partir des bibliothèques combinatoires de protéines dotées d'une structure d'échafaudage des domaines par exemple de la lectine de type C Withdrawn EP2284270A3 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DKPA200001872 2000-12-13
US27209801P 2001-02-28 2001-02-28
EP01270552A EP1341912B1 (fr) 2000-12-13 2001-12-13 Methode pour l'identification et l'isolation des polypeptides de bibliotheques combinatoires de proteines presentant la structure en echafaudage des domaines lectonoides de type c
EP09160663A EP2105503B1 (fr) 2000-12-13 2001-12-13 Procédé d'identification et d'isolation des polypeptides de liaison à partir des bibliothèques combinatoires de protéines dotées d'une structure d'échafaudage des domaines par exemple de la lectine de type C

Related Parent Applications (3)

Application Number Title Priority Date Filing Date
EP09160663A Division-Into EP2105503B1 (fr) 2000-12-13 2001-12-13 Procédé d'identification et d'isolation des polypeptides de liaison à partir des bibliothèques combinatoires de protéines dotées d'une structure d'échafaudage des domaines par exemple de la lectine de type C
EP01270552.1 Division 2001-12-13
EP09160663.2 Division 2009-05-19

Publications (2)

Publication Number Publication Date
EP2284270A2 EP2284270A2 (fr) 2011-02-16
EP2284270A3 true EP2284270A3 (fr) 2012-07-25

Family

ID=32668608

Family Applications (1)

Application Number Title Priority Date Filing Date
EP10185435A Withdrawn EP2284270A3 (fr) 2000-12-13 2001-12-13 Procédé d'identification et d'isolation des polypeptides de liaison à partir des bibliothèques combinatoires de protéines dotées d'une structure d'échafaudage des domaines par exemple de la lectine de type C

Country Status (3)

Country Link
US (1) US20040132094A1 (fr)
EP (1) EP2284270A3 (fr)
ZA (1) ZA200304459B (fr)

Families Citing this family (28)

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Publication number Priority date Publication date Assignee Title
US20040175756A1 (en) * 2001-04-26 2004-09-09 Avidia Research Institute Methods for using combinatorial libraries of monomer domains
US20050089932A1 (en) * 2001-04-26 2005-04-28 Avidia Research Institute Novel proteins with targeted binding
US20100028995A1 (en) * 2004-02-23 2010-02-04 Anaphore, Inc. Tetranectin Trimerizing Polypeptides
US20110086770A1 (en) * 2009-10-09 2011-04-14 Anaphore, Inc. Combinatorial Libraries Based on C-type Lectin-like Domain
US20110086806A1 (en) * 2009-10-09 2011-04-14 Anaphore, Inc. Polypeptides that Bind IL-23R
US10208125B2 (en) 2013-07-15 2019-02-19 University of Pittsburgh—of the Commonwealth System of Higher Education Anti-mucin 1 binding agents and uses thereof
CA2971419C (fr) 2014-12-24 2024-06-18 Neximmune, Inc Compositions de nanoparticules et methodes pour l'immunotherapie
WO2017077382A1 (fr) 2015-11-06 2017-05-11 Orionis Biosciences Nv Protéines chimères bifonctionnelles et leurs utilisations
MX2018006194A (es) 2015-11-19 2018-12-06 Asclepix Therapeutics Llc Peptidos con propiedades antiangiogenicas, antilinfangiogenicas y antiedemicas y formulaciones de nanoparticulas.
CN116769054A (zh) 2016-02-05 2023-09-19 奥里尼斯生物科学私人有限公司 双特异性信号传导剂及其用途
CA3016849A1 (fr) 2016-03-07 2017-09-14 Vib Vzw Anticorps a domaine unique de liaison a cd20
CN109563141A (zh) 2016-05-13 2019-04-02 奥里尼斯生物科学公司 对非细胞结构的治疗性靶向
US11236141B2 (en) 2016-05-13 2022-02-01 Orionis Biosciences BV Targeted mutant interferon-beta and uses thereof
EP3522906B1 (fr) 2016-10-04 2022-03-23 Asclepix Therapeutics, Inc. Composés et procédés d'activation de la signalisation tie2
ES2917000T3 (es) 2016-10-24 2022-07-06 Orionis Biosciences BV Interferón-gamma mutante diana y usos del mismo
CN110573172A (zh) 2017-02-06 2019-12-13 奥里尼斯生物科学有限公司 靶向的工程化干扰素及其用途
AU2018216032B2 (en) 2017-02-06 2022-04-07 Orionis Biosciences BV Targeted chimeric proteins and uses thereof
EP3580230A1 (fr) 2017-02-07 2019-12-18 VIB vzw Protéines chimères bispécifiques ciblées par des cellules immunitaires, et utilisations associées
WO2019028427A1 (fr) 2017-08-03 2019-02-07 Asclepix Therapeutics, Llc. Procédés d'identification et de préparation d'agents pharmaceutiques pour activer le récepteur tie2
WO2019075056A1 (fr) 2017-10-10 2019-04-18 The Johns Hopkins University Particules biomimétiques biodégradables
CN112074267B (zh) 2018-02-05 2024-06-28 奥里尼斯生物科学公司股份有限公司 成纤维细胞结合剂及其用途
EA202192146A1 (ru) 2019-02-15 2021-11-09 Интиграл Молекьюлар, Инк. Антитела к клаудину 6 и их применение
CN113661175A (zh) 2019-02-15 2021-11-16 整体分子公司 包含共同轻链的抗体及其用途
GB202004514D0 (en) 2020-03-27 2020-05-13 Inst De Medicina Molecular Joaeo Lobo Antunes Treatment of Immunosuppressive Cancer
WO2024008755A1 (fr) 2022-07-04 2024-01-11 Vib Vzw Anticorps de traversée de barrière de fluide céphalorachidien
US20240228620A1 (en) 2022-10-06 2024-07-11 Bicara Therapeutics Inc. Multispecific proteins and related methods
WO2024118771A1 (fr) 2022-11-30 2024-06-06 Integral Molecular, Inc. Anticorps dirigés contre la claudine 6, notamment des formats bispécifiques de ceux-ci
WO2024208816A1 (fr) 2023-04-03 2024-10-10 Vib Vzw Anticorps traversant la barrière hémato-encéphalique

Citations (5)

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Publication number Priority date Publication date Assignee Title
WO1994018227A2 (fr) * 1993-02-04 1994-08-18 Denzyme Aps Procede ameliore de repliement de proteines
WO1998056906A1 (fr) * 1997-06-11 1998-12-17 Thoegersen Hans Christian Module formant des trimeres
WO1999024617A1 (fr) * 1997-11-06 1999-05-20 Ventana Genetics, Inc. Procedes et compositions destines a des banques de peptides affichees sur des echafaudages emetteurs de lumiere
EP0947582A1 (fr) * 1998-03-31 1999-10-06 Innogenetics N.V. Une structure polypeptidique utilisable comme un squelette
WO2000020574A2 (fr) * 1998-10-08 2000-04-13 Rigel Pharmaceuticals, Inc. Fusion de proteines d'echafaudage avec des banques de peptides aleatoires

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WO1993022436A1 (fr) 1992-04-30 1993-11-11 Genentech, Inc. Variants du domaine lectine de la selectine

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994018227A2 (fr) * 1993-02-04 1994-08-18 Denzyme Aps Procede ameliore de repliement de proteines
WO1998056906A1 (fr) * 1997-06-11 1998-12-17 Thoegersen Hans Christian Module formant des trimeres
WO1999024617A1 (fr) * 1997-11-06 1999-05-20 Ventana Genetics, Inc. Procedes et compositions destines a des banques de peptides affichees sur des echafaudages emetteurs de lumiere
EP0947582A1 (fr) * 1998-03-31 1999-10-06 Innogenetics N.V. Une structure polypeptidique utilisable comme un squelette
WO2000020574A2 (fr) * 1998-10-08 2000-04-13 Rigel Pharmaceuticals, Inc. Fusion de proteines d'echafaudage avec des banques de peptides aleatoires

Non-Patent Citations (7)

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Title
DOI N ET AL: "SCREENING OF CONFORMATIONALLY CONSTRAINED RANDOM POLYPEPTIDE LIBRARIES DISPLAYED ON A PROTEIN SCAFFOLD", CMLS CELLULAR AND MOLECULAR LIFE SCIENCES, BIRKHAUSER VERLAG, BASEL, CH, vol. 54, no. 5, May 1998 (1998-05-01), pages 394 - 404, XP000891835, ISSN: 1420-682X *
DRICKAMER KURT: "C-type lectin-like domains.", CURRENT OPINION IN STRUCTURAL BIOLOGY, vol. 9, no. 5, October 1999 (1999-10-01), pages 585 - 590, XP002175480, ISSN: 0959-440X *
GRAVERSEN JONAS HEILSKOV ET AL: "Mutational analysis of affinity and selectivity of kringle-tetranectin interaction. Grafting novel kringle affinity onto the tetranectin lectin scaffold.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 275, no. 48, 1 December 2000 (2000-12-01), pages 37390 - 37396, XP002175478, ISSN: 0021-9258 *
GRAVERSEN JONAS HEILSKOV ET AL: "The plasminogen binding site of the C-type lectin tetranectin is located in the carbohydrate recognition domain, and binding is sensitive to both calcium and lysine.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 273, no. 44, 30 October 1998 (1998-10-30), pages 29241 - 29246, XP002175479, ISSN: 0021-9258 *
IOBST SUSANNE T ET AL: "Binding of sugar ligands to Ca-2+-dependent animal lectins: I. Analysis of mannose binding by site-directed mutagenesis and NMR.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 269, no. 22, 1994, pages 15505 - 15511, XP002209823, ISSN: 0021-9258 *
IOBST SUSANNE T ET AL: "Binding of sugar ligands to Ca-2+-dependent animal lectins: II. Generation of high-affinity galactose binding by site-directed mutagenesis.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 269, no. 22, 1994, pages 15512 - 15519, XP002209824, ISSN: 0021-9258 *
TORGERSEN DAWN ET AL: "Mechanism of ligand binding to E- and P-selectin analyzed using selectin/mannose-binding protein chimeras.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 273, no. 11, 13 March 1998 (1998-03-13), pages 6254 - 6261, XP002209822, ISSN: 0021-9258 *

Also Published As

Publication number Publication date
ZA200304459B (en) 2004-05-26
US20040132094A1 (en) 2004-07-08
EP2284270A2 (fr) 2011-02-16

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