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EP1390484A4 - Methods for the identification of inhibitors of pectin esterase expression or activity in plants - Google Patents

Methods for the identification of inhibitors of pectin esterase expression or activity in plants

Info

Publication number
EP1390484A4
EP1390484A4 EP02749538A EP02749538A EP1390484A4 EP 1390484 A4 EP1390484 A4 EP 1390484A4 EP 02749538 A EP02749538 A EP 02749538A EP 02749538 A EP02749538 A EP 02749538A EP 1390484 A4 EP1390484 A4 EP 1390484A4
Authority
EP
European Patent Office
Prior art keywords
inhibitors
plants
identification
activity
methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP02749538A
Other languages
German (de)
French (fr)
Other versions
EP1390484A2 (en
Inventor
Adel Zayed
Robert Ascenzi
Douglas Boyes
Rao Mulpuri
Neil Hoffman
Keith Davis
Jorn Gorlach
Jeffrey Woessner
Carol Hamilton
Kenneth Phillips
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cogenics Icoria Inc
Original Assignee
Paradigm Genetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Paradigm Genetics Inc filed Critical Paradigm Genetics Inc
Publication of EP1390484A2 publication Critical patent/EP1390484A2/en
Publication of EP1390484A4 publication Critical patent/EP1390484A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/04—Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP02749538A 2001-05-30 2002-05-29 Methods for the identification of inhibitors of pectin esterase expression or activity in plants Withdrawn EP1390484A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US29448701P 2001-05-30 2001-05-30
US294487P 2001-05-30
PCT/US2002/016648 WO2002097040A2 (en) 2001-05-30 2002-05-29 Methods for the identification of inhibitors of pectin esterase expression or activity in plants

Publications (2)

Publication Number Publication Date
EP1390484A2 EP1390484A2 (en) 2004-02-25
EP1390484A4 true EP1390484A4 (en) 2005-01-26

Family

ID=23133648

Family Applications (1)

Application Number Title Priority Date Filing Date
EP02749538A Withdrawn EP1390484A4 (en) 2001-05-30 2002-05-29 Methods for the identification of inhibitors of pectin esterase expression or activity in plants

Country Status (3)

Country Link
EP (1) EP1390484A4 (en)
AU (1) AU2002320039A1 (en)
WO (1) WO2002097040A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0580252A2 (en) * 1992-07-20 1994-01-26 Quest International B.V. Improvements in or relating to pectin methyl esterase
US5296376A (en) * 1986-11-04 1994-03-22 Imperial Chemical Industries Plc DNA, constructs, cells and plants derived therefrom

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5413937A (en) * 1986-11-11 1995-05-09 Zeneca Limited DNA constructs containing segments from tomato polygalacturonase and pectin esterase genes
US5298397A (en) * 1989-10-19 1994-03-29 Yamasa Shoyu Kabushiki Kaisha Method of assaying d-vanillylmandelic acid
GB9127098D0 (en) * 1991-12-20 1992-02-19 Ici Plc Dna,dna constructs,cells and plants derived therefrom
GB9403406D0 (en) * 1994-02-23 1994-04-13 Zeneca Ltd DNA,DNA constructs, cells and plants derived therefrom
CN1229495C (en) * 1996-02-21 2005-11-30 诺沃奇梅兹有限公司 Enzyme with pectin esterase activity

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5296376A (en) * 1986-11-04 1994-03-22 Imperial Chemical Industries Plc DNA, constructs, cells and plants derived therefrom
EP0580252A2 (en) * 1992-07-20 1994-01-26 Quest International B.V. Improvements in or relating to pectin methyl esterase

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
CAMARDELLA LAURA ET AL: "Kiwi protein inhibitor of pectin methylesterase: Amino-acid sequence and structural importance of two disulfide bridges", EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 267, no. 14, July 2000 (2000-07-01), pages 4561 - 4565, XP002306978, ISSN: 0014-2956 *
MANGOS T J ET AL: "A spectrophotometric assay for the enzymatic demethoxylation of pectins and the determination of pectinesterase activity.", ANALYTICAL BIOCHEMISTRY. 15 JAN 1997, vol. 244, no. 2, 15 January 1997 (1997-01-15), pages 357 - 366, XP002307175, ISSN: 0003-2697 *
RICHARD L ET AL: "Clustered genes within the genome of Arabidopsis thaliana encoding pectin methylesterase-like enzymes.", GENE. 8 MAY 1996, vol. 170, no. 2, 8 May 1996 (1996-05-08), pages 207 - 211, XP002307177, ISSN: 0378-1119 *
SEKHON H S ET AL: "IN-VITRO EFFECTS OF HERBICIDES ON FUNGAL PECTOLYTIC ENZYMES", PROCEEDINGS OF THE AMERICAN PHYTOPATHOLOGICAL SOCIETY, vol. 1, 1974, pages (1975), XP009040441 *
WOJCIECHOWSKI C L ET AL: "A continuous fluorometric assay for pectin methylesterase.", ANALYTICAL BIOCHEMISTRY. 15 MAY 1996, vol. 237, no. 1, 15 May 1996 (1996-05-15), pages 103 - 108, XP002307176, ISSN: 0003-2697 *

Also Published As

Publication number Publication date
WO2002097040A2 (en) 2002-12-05
WO2002097040A3 (en) 2003-10-02
EP1390484A2 (en) 2004-02-25
AU2002320039A1 (en) 2002-12-09

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Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20031015

AK Designated contracting states

Kind code of ref document: A2

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

AX Request for extension of the european patent

Extension state: AL LT LV MK RO SI

A4 Supplementary search report drawn up and despatched

Effective date: 20041210

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: ICORIA, INC.

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20051201